Insulin pdx 1 glucose transporter type 2. Medical search. Frequent questions

In pancreatic islets, GLUT2 allows a rapid equilibration of glucose between the extracellular space and the interior of the cells and it may play a crucial role in the glucose signaling mechanism leading to insulin secretion (43). (justia.com)
Transgenic mice in which Hnf6 expression is maintained in postnatal islets ( pdx1 PB Hnf6 ) show overt diabetes and impaired glucose-stimulated insulin secretion (GSIS) at weaning. (diabetesjournals.org)
Nutrient-stimulated insulin secretion is dependent upon the generation of metabolic coupling factors in the mitochondria of the pancreatic B cell. (123dok.net)
To investigate the role of Ca2+ in mitochondrial function, insulin secretion from INS-1 cells stably expressing the Ca2+-sensitive photoprotein aequorin in the appropriate compartments was correlated with changes in cytosolic calcium ([Ca2+]c) and mitochondrial calcium ([Ca2+]m). (123dok.net)
Glucose-induced increases in [Ca2+]m, [Ca2+]c, and insulin secretion all demonstrate a pronounced initial peak followed by a sustained plateau. (123dok.net)
Finally, [Ca2+]m, [Ca2+]c, and insulin secretion also display desensitization phenomena following repeated additions of the three stimuli. (123dok.net)
Maturity-onset diabetes of the young 10 (MODY10) [MIM:613370]: A form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. (diabetestalk.net)

The results also showed a protective effect on endogenous pancreatic insulin secretory capacity owing to reduced insulin requirement. (bvsalud.org)
Sustained expression of Hnf6 also results in downregulation of the β-cell-specific transcription factor MafA and a decrease in total pancreatic insulin. (diabetesjournals.org)

Description: Pancreatic duodenal homeobox-1 protein (PDX-1), also designated Insulin promoter factor (IPF1), Insulin upstream factor 1 (IUF1), somatostatin transactivating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic cells. (lifchem.com)

Metformin, an oral medicine used to treat type 2 diabetes, is a Glucagon-Like Peptide-1 (GLP-1) analogue, which has been demonstrated to stimulate the expression of Pancreatic duodenal homeobox-1 (Pdx-1), Insulin and Glucose transporter 2 (Glut-2) genes. (ac.ir)
Description: PDX1 (pancreatic and duodenal homeobox 1), also known as insulin promoter factor 1, is a transcription factor in the ParaHox gene cluster. (lifchem.com)

In this study, the regulatory effect of metformin on beta cells function through the expression of � Pdx-1, Insulin and Glut-2 genes was investigated. (ac.ir)
The dissected pancreases were then used for assessment of Pdx-1, Insulin and Glut-2 genes expression by semi-quantitative RT-PCR method. (ac.ir)
The regulatory effect of metformin on beta cells function might not be related to the expression of Pdx-1, Insulin and Glut-2 genes and may be related to the expression of gloconeogenesis pathway genes. (ac.ir)
The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. (lifchem.com)
Transcriptional activator Islet-Brain 1 (IB1) is disclosed which is involved in the control of the GLUT2 and insulin genes by interacting with homologous cis-regulatory elements of the GLUT2 and insulin promoters, and to materials and methods deriving from this work. (justia.com)
The present invention is based on successful expression cloning of a transcription factor that binds to the GTII element of the GLUT2 and insulin genes from a differentiated insulin-secreting cDNA library. (justia.com)
The largest chromosome, chromosome 1, contains about 8000 genes. (diabetestalk.net)
The rat, mouse, and at least 3 fish species have 2 insulin genes (Lomedico et al. (diabetestalk.net)
1993) showed that in the mouse embryo the 2 proinsulin genes are regulated independently, at least in part. (diabetestalk.net)
A) Luciferase reporter genes were determined in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with Glutaminase-IN-1 different concentrations of reporter and myc-ZKSCAN5. (bms-911543.com)

This factor is abundantly expressed in the pancreatic islets and in the brain and has been named IB-1 for Islet-Brain 1. (justia.com)
This study evaluated whether the supplementation of necrostatin‐1 (Nec‐1), a potent inhibitor of necroptosis, to islet culture media could improve the recovery, maturation, and function of pre‐weaned porcine islets (PPIs). (crenolanibinhibitor.com)
Methods: PPIs were isolated from pre‐weaned Yorkshire piglets (8‐15 days old) and either cultured in control islet culture media (n = 6) or supplemented with Nec‐1 (100 µM, n = 5). (crenolanibinhibitor.com)
Islet recovery, viability, and oxygen consumption rate were unaffected by Nec‐1. (crenolanibinhibitor.com)
Conclusion: This study underlines the importance of necroptosis in islet cell death after isolation and demonstrates the novel effects of Nec‐1 to increase islet insulin content, enhance pancreatic endocrine cell development, facilitate GLUT2 upregula‐ tion in beta cells, and augment insulin secretion. (crenolanibinhibitor.com)
Nec‐1 supplementation to culture media significantly improves islet quality prior to xenotransplantation. (crenolanibinhibitor.com)

The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. (lifchem.com)
The GLUT2 facilitated glucose transporter isoform is a membrane protein present in the pancreatic .beta. (justia.com)
Serum insulin-like growth factor (IGF)-I and IGF-binding protein-3 concentrations and prostate cancer risk: results from the European Prospective Investigation into Cancer and Nutrition. (mdc-berlin.de)
So, for example, one gene will code for the protein insulin, which is important role in helping your body to control the amount of sugar in your blood. (diabetestalk.net)
D) Cytoplasmic and nuclear ZKSCAN5 protein levels in two types of breast cancer cell lines, ZR75-1 and MDA-MB-231. (bms-911543.com)

Furthermore, transgenic mice that express GLUT2 antisense RNAs driven by the insulin promoter led to an 80% reduction in GLUT2 which was paralleled by a decreased glucose-induced insulin secretory response and by the onset of diabetes (48). (justia.com)

In patients with type two diabetes mellitus, diabetic diets containing 8.5 g D-allulose were effective in improving PPG levels. (bvsalud.org)
Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (IDDM), as well as maturity onset diabetes of the young type 4 (MODY4). (lifchem.com)
Diabetes mellitus, insulin-dependent, 2 (IDDM2) [MIM:125852]: A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. (diabetestalk.net)
Diabetes mellitus, permanent neonatal (PNDM) [MIM:606176]: A rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. (diabetestalk.net)

Comparison of peak postprandial blood glucose (PPG) levels after consumption of a standard diabetic diet and a diabetic diet containing 8.5 g of D-allulose was the primary endpoint. (bvsalud.org)
Practicing late suhoor (p = 0.012) and self-monitoring of blood glucose (SMBG) (p = 0.026) during Ramadan were significantly associated with a better diabetes knowledge score. (bvsalud.org)
Failure to maintain an optimal circulating blood glucose concentration results in diabetes, a disease with varying etiologies. (diabetesjournals.org)
The peptide hormone insulin, produced by pancreatic β-cells, is critical for maintaining normal blood glucose levels and acts to regulate cellular metabolism and growth by facilitating glucose uptake by peripheral tissues. (diabetesjournals.org)

insulin-secreting cells, the basolateral membrane of intestinal and kidney absorptive cells, in hepatocytes and in a subset of neurons (21,31,44). (justia.com)
A fragment of the murine GLUT2 promoter has been cloned and shown to be glucose-responsive when transfected into differentiated insulin-producing cells or into hepatocytes (35,36,52). (justia.com)
HGF was cloned as a growth factor for hepatocytes ( 1 , 2 ), is identical to scatter factor (SF) and was originally discovered as a fibroblast-derived cell motility factor for epithelial cells ( 3 ). (spandidos-publications.com)

A better understanding of the levers governing transcription factor activity benefits our ability to generate therapeutic cell types at will. (silverchair.com)

Glucagon-like peptide-1 receptor agonist (GLP-1RA) has been clinically proven to protect endothelial function. (bvsalud.org)
exon 2 encodes the signal peptide, the B chain, and part of the C-peptide, while exon 3 encodes the remainder of the C-peptide and the A chain (Steiner and Oyer, 1967). (diabetestalk.net)

Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. (lifchem.com)

In these cells, GLUT2 catalyzes the transepithelial transport of glucose. (justia.com)
On the other hand, insulinoma cells that had lost their normal glucose responsiveness have low GLUT2 content, but some glucose sensitivity may be recovered after reintroducing GLUT2 expression through stable transfection of these cells (10,16). (justia.com)
When tested in vitro, IB1 bound specifically to the GTII cis element of the GLUT2 gene and to an homologous regulatory sequence of the insulin promoter termed RIPE3. (justia.com)
On days 3 and 7 of culture, islets were assessed for recovery, insulin content, viability, cellular composition, GLUT2 expression in beta cells, differentia‐ tion of pancreatic endocrine progenitor cells, function, and oxygen consumption rate. (crenolanibinhibitor.com)
Additionally, beta cells of Nec‐1‐treated islets had a significant upregulation in GLUT2 expression. (crenolanibinhibitor.com)
The enhanced development of major endocrine cells and GLUT2 expression after Nec‐1 treatment subsequently led to a significant increase in the amount of insulin secreted in response to in vitro glucose challenge. (crenolanibinhibitor.com)

Further, the hSR-BI/CLA-1 transcriptional activity was enhanced by exendin-4, which was diminished by the inhibition of AMPK or dominant-negative AMPK-α-subunit. (bvsalud.org)

In this study, the efficacy of a diabetic diet containing D-allulose was investigated in patients with type 2 diabetes using an intermittently scanned continuous glucose monitoring system (isCGM). (bvsalud.org)
BACKGROUND AND AIMS: To assess the level of diabetes knowledge and its association with diabetes self-management practices during Ramadan fasting among patients with type 2 diabetes (T2D). (bvsalud.org)

A D-allulose-containing diabetic diet improved PPG levels in type two diabetes patients compared with a strictly energy-controlled diabetic diet. (bvsalud.org)
It enables considered Initials Only 2007 of website doomed websites bring in the extract of Diabetic transporter of its mellitus. (babyfreunde.de)
We are that titles and diabetic numbers will however raise from the Initials Only, which targets both providers and most Real contributions in necessary editors to be studied by the insulin. (babyfreunde.de)

Conditional Met KO mice were generated using Cre‑loxP methodology and characterization of these mice indicated that the HGF‑Met signaling pathway is essential in regeneration, protection, and homeostasis in various tissue types and cells. (spandidos-publications.com)

however, there is also a risk that artificial sweeteners exacerbate glucose metabolism. (bvsalud.org)

It encodes the inactive pre-pro-HGF, a single chain of 728 amino acids (83 kDa), which includes a signal sequence ( 1 - 31 ), a heavy α chain (69 kDa), and a light β chain (34 kDa). (spandidos-publications.com)

In summary, our results proved that exendin-4 increased hSR-BI/CLA-1 expression via the AMPK/FoxO1 pathway to activate eNOS, providing a basic mechanism underlining the protective effect of GLP-1RA on endothelial function. (bvsalud.org)

Transdifferentiation is a type of cellular reprogramming involving the conversion of one differentiated cell type to another. (silverchair.com)

Various growth factors, cytokines, and prostaglandins upregulate HGF gene expression, including basic fibroblast growth factor, oncostatin M, hypoxia-inducible factor 1α and nuclear factor-κB (NF-κB) ( 9 ). (spandidos-publications.com)

B) Luciferase reporter gene detection in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with expression in ZR75-1 and MDA-MB-231 cells, which were transfected with ZKSCAN5 shRNA, control shRNA, or ZKSCAN5 shRNA plus shRNA-resistant ZKSCAN5 (ZKSCAN5-R). The representative Western blot further showed the expression of ZKSCAN5. (bms-911543.com)

Plasminogen activator inhibitor-1 inhibits plasminogen activators and subsequent fibrinolysis potentiating thromboembolic effects. (gremjournal.com)
Crenolanib inhibits both wild type FLT3 and its constitutively active mutations. (crenolanibinhibitor.com)

Proinsulin is converted to insulin by the enzymatic removal of a segment that connects the amino end of the A chain to the carboxyl end of the B chain. (diabetestalk.net)

We provide evidence that continued expression of Hnf6 impairs GSIS by altering insulin granule biosynthesis, resulting in a reduced response to secretagogues. (diabetesjournals.org)
The L-type Ca2+-channel blocker, SR7037, abolished the effects of glucose and KCl while attenuating the CCh response. (123dok.net)

Important cis-regulatory sequences were identified within this promoter region including a functionally responsive PDX-1 element, a cyclic AMP responsive element, and three cis elements termed GTI, GTII and GTIII (3,36,53). (justia.com)

Hyperinsulinism decreases: the number of insuin receptors, insulin-like factor receptors, insulin-like growth factor binding globulin-1 and sex hormone binding globulin. (gremjournal.com)

Here, we investigated the effect of GLP-1RA and exendin-4 on the expression of hSR-BI/CLA-1 in HUVECs. (bvsalud.org)
Next, exendin-4 increased the expression of hSR-BI/CLA-1 and a blockade of GLP-1R cancelled this effect. (bvsalud.org)
Mutation of FoxO1-binding or silencing of FoxO1 cancelled the effect of exendin-4 on hSR-BI/CLA-1 expression. (bvsalud.org)
These results suggest that downregulation of Hnf6 expression in β-cells during development is essential to achieve a mature, glucose-responsive β-cell. (diabetesjournals.org)
A, B) Cell proliferation and colony formation assays in HLECs cultured in conditioned medium come from ZR75-1 or MDA-MB-231 cells stably infected with lentivirus carrying ZKSCAN5 shRNA or ZKSCAN5 shRNA plus ZKSCAN5-R. The representative Western blot displays the expression of ZKSCAN5. (bms-911543.com)

Next, ChIP assay demonstrated that exendin-4 increased the FoxO1-binding in the hSR-BI/CLA-1 promoter by upregulation of FoxO1. (bvsalud.org)

Pregnant C57BL/6 mice were randomly divided into 2 groups. (ac.ir)
Furthermore, the results of studies using HGF‑overexpressing mice have indicated the therapeutic potential of HGF for various types of disease and injury. (spandidos-publications.com)
For tumour estimation, nude mice were inoculated subcutaneously with 1 107 ZR75-1 cells with different constructs on the right side. (bms-911543.com)
BALB/c mice were injected with 1 106 MDA-MB-231 cells labelled with luciferase carrying the indicated constructs into the lateral tail vein. (bms-911543.com)

Tumour Growth and Metastasis Analysis The animal study was approved and Glutaminase-IN-1 monitored by the Ethics Committee of Harbin Medical University Cancer Hospital (the ID of animal experiment ethical approval: SYDW2021-056). (bms-911543.com)

chains A and B are derived from a 1-chain precursor, proinsulin, which was discovered by Steiner and Oyer (1967). (diabetestalk.net)

pancreatic cells to glucose remains debated. (justia.com)
When compared to untreated islets, Nec‐1 treatment dou‐ bled the beta‐ and alpha‐cell composition and accelerated the development of delta cells. (crenolanibinhibitor.com)
Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulfide bonds. (diabetestalk.net)
The conditioned medium from these cells re-expressing ZKSCAN5 could rescue these effects ( Figures?2A, B ). A similar tendency was also detected in HLEC migration analysis ( Figures?2C, D ). Open in a separate window Figure?2 VEGFC secreted by cancer cells, under the influence of ZKSCAN5, regulates HLEC proliferation, migration, and tube formation. (bms-911543.com)
p 0.01 versus the control shRNA group (A, B). (C, D) Wound healing assays for HLECs cultured in conditioned medium from ZR75-1 or MDA-MB-231 cells, which were Glutaminase-IN-1 stably infected as in (A). The image shown is one of the representative results (C, D). Scale bar: 100 m. (bms-911543.com)
VEGF-D (E, F) Tube formation assays for HLECs cultured in the conditioned medium from ZR75-1 or MDA-MB-231 cells, which were stably infected as in (A). All values shown are the mean SD of triplicate measurements and were repeated three times with analogous. (bms-911543.com)

This rat insulin promoter element 3 (RIPE3) is an important enhancer sequence sufficient to confer .beta. (justia.com)

Previously, we demonstrated that endothelial NO synthase (eNOS) was activated by high-density lipoprotein (HDL) via its scavenger receptor of the B class/human homologue of SR-BI, CD36 and LIMPII analogous-1(hSR-BI/CLA-1). (bvsalud.org)
Human TNFa Immunoassay KitEA 9,360.00 766Singulexmillipore 03-0024-00ReagentsActive GLP-1 Immunoassay Kit AssemblyEA 9,360.00 766Singulexmillipore 03-0024-01ReagentsActive GLP-1 Immunoassay KitEA 14,040.00 766Singulexmillipore 03-0024-02ReagentsActive GLP-1 Immunoassay KitEA 14,040.00 766Singulexmillipore 03-0024-03ReagentsErenna? (thesixteendigital.com)

We consider how, over the last 20 years, advances in genetic engineering and cell culture techniques have improved the efficiency and efficacy of the transdifferentiation process and brought us closer to a clinically relevant therapy for type 1 diabetes (T1D). (silverchair.com)
Glucose and KCl, which depolarize the cell membrane, as well as the Ca2+-mobilizing agonist, carbachol (CCh), cause substantial increases in [Ca2+]m which are associated with smaller rises in [Ca2+]c. (123dok.net)
The ZKSCAN5 knockdown ZR75-1 or MDA-MB-231 cell-conditioned medium decreased HLEC proliferation. (bms-911543.com)

Results: Nec‐1 supplementation induced a 2‐fold increase in the insulin content of PPIs on day 7 of culture. (crenolanibinhibitor.com)

Additionally, the HGF‑Met signaling pathway is correlated with the biology of cancer types, neurons and immunity. (spandidos-publications.com)
Free Radical Biology and Medicine 42 (1): 32-43. (mdc-berlin.de)
Journal of Structural Biology 159 (1): 158-163. (mdc-berlin.de)

Informed consent was obtained from the patients, and all study protocols were approved by the Institutional Review or Glutaminase-IN-1 Committees of Harbin Medical University Cancer Hospital. (bms-911543.com)

There are four types of base: adenine (A), thymine (T), guanine (G) and cytosine (C). These bases link in a very specific way: A always pairs with T, and C always pairs with G. The DNA molecule has two important properties. (diabetestalk.net)

Journal of Immunotherapy 30 (1): 29-39. (mdc-berlin.de)
European Journal of Immunology 37 (1): 100-109. (mdc-berlin.de)

Anti-ZKSCAN5 (SAB4501021), anti-VEGFC (ab83905), and anti-LYVE1 (ab10278) primary antibodies were used at 1:100, 1:100, and 1:50 dilutions, respectively. (bms-911543.com)

The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. (lifchem.com)

Figure 1. (spandidos-publications.com)

Anatomy17

Organisms9

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Analytical, Diagnostic and Therapeutic Techniques and Equipment16

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