Insect cells cells cultured. Medical search. Frequent questions

PEPCF expresses proteins in bacteria, insect and mammalian cells and uses a variety of chromatographic and biophysical techniques for protein purification and characterization. (embl.org)
Interactions between light and various cells in cultures, such as bacteria or mammalian cells, are widely applied for optical sensors and optofluidic systems. (mdpi.com)
Biological contaminants fall into two categories: those that are easy to detect (such as bacteria, molds, and yeast), and those that are difficult to detect (such as viruses, protozoa, mycoplasmas, other cell lines, and insects). (corning.com)
Bacteria, molds, and yeast are the most common contaminants in cell culture environments. (corning.com)
While the "classical" animal Rhodopsin in the photoreceptor cell of the eye represents prototypical G-protein coupled receptors, microbial Rhodopsins prevalently present in Achaea, Bacteria, and lower Eukaryotes show a wide variation of functionalities [1]. (hu-berlin.de)
Mycoplasma and Acholeplasma are genera that comprise more than 100 recognized species in the class Mollicutes, which are bacteria that lack a cell wall. (invivogen.com)
Unlike bacteria or fungi, mycoplasmas do not cause consistently observable alterations in cell culture such as rapid pH change or culture turbidity [2,4] . (invivogen.com)
The Genomic DNA Mini Kits for Blood and Cultured Cells provide a fast and economical method for the purification of total DNA (including genomic, mitochondrial, and viral DNA) from fresh whole blood, plasma, serum, buffy coat, other bodily fluids, lymphocytes bacteria, and cultured cells. (primelabmed.com)
They are ideal for bacteria, nematodes (C. elegans), insects (Drosophila), amphibians (Xenopus), fish (zebrafish) and plant cell culture (Arabidopsis). (tritechresearch.com)
Weevils - one of the most specious clades of insects in the world - relies on bacteria for B vitamins that are missing from their plant-based diets. (lu.se)
Insects heavily rely on bacteria for essential nutrients that are lacking in their diet. (lu.se)
Our findings show that bacteria play a crucial role in providing insects with the nutrients they need to survive and thrive," says Charlie Cornwallis, biology researcher at Lund University. (lu.se)
The study, which included Weevils, shows that bacteria consistently provide insects with vitamin B, a vital nutrient they cannot make themselves. (lu.se)
Insects have become so dependent on bacteria that they have developed new organelles to house them - so called bacteria factories. (lu.se)
The nutrients provided by bacteria have enabled insects to survive on highly unbalanced diets and exploit new types of food resources. (lu.se)
However, the effects of bacteria on insect diversity and variation are not straightforward. (lu.se)
The PulseNet specimens have no connection other than symbiotic flora, colonization, contamination, or infection by bacteria that at some point years ago were progeny of the same cell. (cdc.gov)

Insects cell lines are commonly used in place of prokaryotic ones because post-translational modifications of proteins are possible in insect cells whereas this mechanism is not present in prokaryotic systems. (wikipedia.org)
Currently we do not offer protein expression in other host organisms, but we can purify proteins expressed in other host organisms (e.g. yeast, hybridoma cell lines, …) if you are able to provide us with the expression cell pellet (intracellular proteins) or with the cell culture supernatant (secreted proteins). (embl.org)
The facility expresses and purifies proteins from E. coli , insect cells and mammalian cells. (embl.org)
amount of proteins in the culture of mammalian cells and insect cells. (thomassci.com)
Commonly known as HEK293 cells they are actually an excellent protein source, especially for secreted proteins. (uni-konstanz.de)
Quite easy to scale up the volume still using attached cells, in special plastic bottles that present a large surface area: thats most useful especially for secreted proteins. (uni-konstanz.de)
There are two main ways to express your gene in HEK293 cells: Producing intracellular proteins in HEK293 cells requires to grow the cells in suspension which can be a bit more of an art and requires a CO2 shaking incubator. (uni-konstanz.de)
The expression of such genes lead to a robust production of proteins by Sf9 cells. (nexcelom.com)
Similarly to Sf9 and Sf21 cells, the High Five cells are also used in the expression of various proteins. (nexcelom.com)
Similarly coupled proteins may someday be used to produce specialized molecules that deliver lethal drugs only to cancerous cells. (scienceblog.com)
The finding is important as researchers look for what proteins are essential and how they function in cells, potentially providing answers to everything from insect control to the control of human diseases such as smallpox. (scienceblog.com)
The method uses chaotropic salt to lyse cells and degrade proteins. (primelabmed.com)

There are currently more than 100 insect cell lines available for recombinant protein production with lines derived from Bombyx mori, Mamestra brassicae, Spodoptera frugiperda, Trichoplusia ni, and Drosophila melanogaster being of particular interest. (wikipedia.org)
Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). (fishersci.com)
For protein expression we use either the Sf21 or the Hi5 insect cell lines. (embl.org)
In this protein expression process it is vital to know the concentration and viability of the Sf9 cells both for a healthy transfection as well as a robust protein production. (nexcelom.com)
Because cell size has been linked with a healthy viral infection and protein expression measuring and monitoring cell size is an effective tool for monitoring cell culture health. (nexcelom.com)
On the subject of integration and "complete solutions," GE Healthcare's ReadyToProcess™ product line covers all unit operations from media preparation and cell cultivation to protein purification. (genengnews.com)
Various expression systems are used to obtain a protein sample suitable for crystallization, including the pichia pastoris and insect cell/baculovirus system. (hu-berlin.de)
The diffraction images collected from single crystals can be used to calculate the electron density map of the crystal unit cell which finally allows to model the three dimensional structure of the crystallized protein. (hu-berlin.de)
Nevertheless, mycoplasmas can have disastrous effects on eukaryotic cells, as they can alter every cellular function (proliferation, protein synthesis, susceptibility to viral infection, etc. (invivogen.com)
The phylogenetic distance between humans and hexapods gives good reasons to think that zoonoses are unlikely, and Ÿnsect's scientific teams continuously study mealworms and assess potential risks," Florent Dupriez, project manager, insect health, for France-based insect protein and oil producer Ÿnsect, wrote in an email. (petfoodindustry.com)
Expression is screened on a small scale in 4-10 ml cultures and soluble and insoluble cellular fractions are analyzed by SDS-PAGE for protein expression. (weizmann.ac.il)
Once optimal conditions have been determined, large-scale cultures (1-5L) are used to obtain larger amounts of protein. (weizmann.ac.il)
This product is useful as a positive control for ELISA and western blot and for a variety of in vitro applications including cell differentiation, cell signaling, clotting assays, fibrinolysis, plasminogen activation, protein-protein interactions, receptor binding, thromboelastography, and thrombolysis. (innov-research.com)
Roughly 25.4% of the whole-insect genome appears to encode long interspersed nuclear elements (LINEs), and a total of 11,901 protein sequences predicted by automated gene finding exhibited homology with reference sequences in the NCBI NR and/or UniProtKB databases at the most stringent similarity cutoff level (i.e., the gold tier). (usda.gov)
Genes Genes are segments of deoxyribonucleic acid (DNA) that contain the code for a specific protein that functions in one or more types of cells in the body or the code for functional ribonucleic. (msdmanuals.com)

Using isotropic fractionator coupled with immunohistochemistry, we counted the total number of neuronal and non-neuronal cells in the whole brain, central brain, and optic lobe of Drosophila melanogaster. (bvsalud.org)

This information will be used by scientific researchers and stakeholders or parties interested in controlling these invasive insect pests. (usda.gov)

This is usually only tried with mycoplasma infections, as fungus and yeast contaminants are unlikely to be eradicated, bacterial contaminants are often already antibiotic-resistant, and many cultures lose critical characteristics after cleanup. (corning.com)
These include bacterial system and three eukaryotic systems, such as yeast, insect and mammalian cells. (weizmann.ac.il)

Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
These loosely adherent to suspension cell lines can be easily imaged on any Cellometer to determine cell concentration, viability and size. (nexcelom.com)
Flask-shaker with a suspension culture of insect-cells expressing recombinant microbial Rhodopsin. (hu-berlin.de)

Riboflavin supports cell growth and metabolism, aiding researchers in R&D. In cell culture, it is often found as a key ingredient in media formulations. (sigmaaldrich.com)

Since research organizations have limited personnel and must deal with compliance issues, InvivoGen has developed a simple and practical mycoplasma detection kit, PlasmoTest™, which sits right in the incubator alongside cells. (invivogen.com)

Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
Insects can carry viruses that are dangerous for humans (or farm animals). (petfoodindustry.com)
People may get viruses by swallowing or inhaling them, by being bitten by insects, through sexual contact, or congenitally (passed by a pregnant person to the fetus). (msdmanuals.com)
When the infected host cell dies, it releases new viruses, which go on to infect other cells. (msdmanuals.com)
Some viruses do not kill the cells they infect but instead alter the cell's functions. (msdmanuals.com)

The Sf9 cell line is one of the most commonly used lines in insect cell culture. (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
Grace's Supplemented Insect Medium, 2x contains lactalbumin hydrolysate and yeastolate not commonly found in the unsupplemented version commonly diluted with sterile water, Fetal bovine Serum and agarose before use in the agarose overlay, as described in the product manual. (fishersci.com)

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows . (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
[6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
Tissue culture treated or non-treated flasks available with a vent cap or plug seal cap to meet your needs. (thomassci.com)
The tissue culture treated NEST Multi-layer Flasks enable you to grow more cells faster and easier, thereby making your cell culture workflow more productive, easier, and in a more convenient manner. (thomassci.com)
Researchers found that the rate of virus replication in tissue culture was not affected when MTase1 was removed. (scienceblog.com)

Used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21. (fishersci.com)
Sf9 or Sf21 cells are used for the generation of the recombinant baculoviruses. (embl.org)
Cellometer Auto T4 is used to count cell concentration, viability and also analyze size distribution of insect cells, such as SF9, SF21, and High 5. (nexcelom.com)

For baculovirus-mediated expression in insect cells, we require expression vectors compatible with the Bac-to-Bac system (for example pFastBac, MultiBac, biGBac or MacroBac vectors). (embl.org)
For mammalian cells, we can work with most standard mammalian expression vectors (e.g. based on the pcDNA backbone) or BacMam vectors. (embl.org)
Target genes of interest cloned in the appropriate expression vectors are introduced into host cells. (weizmann.ac.il)
The infective forms of T cruzi are contained in the feces of the insect vectors and gain entry into its mammalian hosts through contamination. (medscape.com)
Epimastigotes are an extracellular and noninfective form of the parasite found in the midgut of insect vectors, where they multiply by binary fission. (medscape.com)

The Sf9 cells are highly susceptible to baculovirus, which is widely used for expression of particular genes in the cultured insect cells. (nexcelom.com)
The histogram above displays the number and size of Sf9 cells infected with baculovirus. (nexcelom.com)
In the histogram above the infected cell population was overlayed with the uninfected cell population to show the shift in cell size after infection with baculovirus. (nexcelom.com)
A rapid method for estimation of baculovirus titer based on viable cell size. (nexcelom.com)

His original idea, presented in 1902, was called totipotentiality: "Theoretically all plant cells are able to give rise to a complete plant. (wikipedia.org)
Many plant defense metabolites are stored as non-toxic glucosides (pro-toxins) and are hydrolyzed to reactive aglucones in the herbivore gut by plant or insect β -glucosidases. (nature.com)
But in most cases, such as plant-feeding insects, dietary specialization is linked to a significant increase in species. (lu.se)

The use of insect cell lines as production hosts is an emerging technology for the production of bio pharmaceuticals. (wikipedia.org)
As an example concerning the SARS-CoV-2 pandemic, studies of researchers from Wageningen University show that insects do not have a receptor capable of binding to SARS-CoV-2, and are therefore not hosts of this virus and preventing the replication of the virus. (petfoodindustry.com)
As with other parasites that infect both mammalian and insect hosts, the life cycle of T cruzi is complex (see image below). (medscape.com)
T cruzi can also be transmitted when mammalian hosts ingest infected insects, and this mechanism of transmission may play a major role in maintaining the sylvatic cycle. (medscape.com)

Among these, about 20 species have been described to be contaminants of eukaryotic cell cultures. (invivogen.com)
Various insect species serve as valuable model systems for investigating the cellular and molecular mechanisms by which a brain controls sophisticated behaviors. (bvsalud.org)
This has allowed insects to access a wide variety of food, leading to remarkable species diversification in some cases, according to a new study from Lund University in Sweden. (lu.se)
Insects are crucial for biodiversity and among the most successful species on the planet. (lu.se)
In certain insect families, specialization on specific diets involving blood has halted species diversification. (lu.se)

Applicable to large-scalely culture adherent cells. (thomassci.com)

A genome sequence was recently reported for a gypsy moth cell line, which was derived from LDD ovary tissues over 40 years ago. (usda.gov)
Doctors may base the diagnosis on symptoms, blood tests and cultures, or examination of infected tissues. (msdmanuals.com)

They are readily detected when antibiotics are not present, but when antibiotics are used routinely in cell culture protocols, it can cause a slow-growing infection that is difficult to detect. (corning.com)
Although there are many insect cells that are routinely studied and used in research, the most common is the Sf9 cell line. (nexcelom.com)

Some DNA differences result from the blunders during the DNA replication necessary for cell division. (cdc.gov)

B. burgdoferi does not grow on an agar plate, and its isolation was challenging before development of insect-based liquid growth media. (cdc.gov)
We describe the isolation and characterization of an insect-specific flavivirus (ISF) from Ochlerotatus caspius (Pallas, 1771) mosquitoes collected in southern Portugal. (unl.pt)

BLV can also infect a variety of cell cultures as well as other animals such as sheep, goats, and chimpanzees. (cdc.gov)
As epimastigotes (depicted in the first image below) move to the hindgut, they differentiate into metacyclic trypomastigotes (depicted in the second image below), which are nondividing forms resistant to mammalian complement that have the capacity to infect mammalian cells. (medscape.com)

Animal steroid hormones initiate signaling by passive diffusion into cells and binding to their nuclear receptors to regulate gene expression. (biologists.com)
In agreement with GSS activity localization in vivo , we identified six genes encoding arylsulfatase-like enzymes with a predicted C-terminal transmembrane domain, of which five showed GSS activity upon heterologous expression in insect cells. (nature.com)
The lab generates transgenic mice with very cell-type-specific alterations in gene expression to determine the roles of those cells and molecules on memory function, using behavioral testing. (tritechresearch.com)
C/EBPbeta overexpression and siRNA knockdown in human lung epithelial cells resulted in suppression and activation of RIG-I expression respectively, implying a negative regulatory role of C/EBPbeta. (cdc.gov)

PlasmoTest™ is based on detection of mycoplasmas by engineered cells that express Toll-like receptor 2 (TLR2) , a pathogen recognition receptor that detects bacterial membranes components. (invivogen.com)

Enzymatic conversion of ADP to ATP can be detected by luciferase-containing kits but requires expensive luminometric equipment and can generate false negatives due to impaired enzyme function [6] or to false positives from eukaryotic ATP in the cell culture medium [5] . (invivogen.com)

The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)

This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)

Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
If biological contaminants are undetected, they can significantly affect your resulting data, rendering it inaccurate or useless, or make any products manufactured by the cultures unusable. (corning.com)
During the culturing process, the use rate of the shaker is significantly increased, and the survival rate and viability of cells are both dramatically elevated. (thomassci.com)
Contrary to urban legend, they are significantly cheaper than insect cells to culture. (uni-konstanz.de)

Several insect herbivores, including the cabbage stem flea beetle ( Psylliodes chrysocephala ), prevent glucosinolate activation by forming desulfo-glucosinolates. (nature.com)

Cell contamination is a major concern in 3D cell culture labs as well as with more traditional 2D monolayer cultures. (corning.com)

Walker thinks that if we can somehow get the HIV-specific T-help back, CTLs capable of attacking and destroying HIV-infected cells will be effectively remobilized and HIV infection will be officially turned into a chronic, manageable viral infection - like herpes or, say, genital warts. (treatmentactiongroup.org)
Randomized controlled trials that measure LCI virus infections (by viral culture or reverse transcription polymerase chain reaction [RT-PCR]) as the outcome provide the best and most persuasive evidence of vaccine efficacy, but such data are not available for all populations. (cdc.gov)
Human Immunodeficiency Virus (HIV) Infection Human immunodeficiency virus (HIV) infection is a viral infection that progressively destroys certain white blood cells and is treated with antiretroviral medications. (msdmanuals.com)

Important abiotic stresses include excess soil acidity or alkalinity, drought, and temperature extremes, while insects and diseases represent major biotic stresses (Pena et al. (springer.com)
We have a convenient, quiet, energy-efficient incubators with heating/cooling temperature control, which is great for maintaining fly stocks, or growing S2 cells. (tritechresearch.com)

contrary to insect cells, the DNA can be used directly for transient transfections . (uni-konstanz.de)

Infected insects take blood meals from humans and their domestic animals and deposit parasite-laden feces. (medscape.com)

For reasons that remain less than clear, there is incongruence between results of serologic testing and results of enrichment blood culture and PCR, which was addressed in our discussion and previous publications ( 7 ). (cdc.gov)
The quality of the Genomic DNA Mini Kits for Blood and Cultured Cells is tested on a lot-to-lot basis by isolating genomic DNA from 200 µl of whole human blood. (primelabmed.com)
[ 1 ] T cruzi mostly is found in blood-sucking triatomine insects (kissing bugs) and small mammals in a sylvatic cycle that is enzootic from the southern and southwestern United States to central Argentina and Chile. (medscape.com)
Here, we uncovered the genetic differences of immune blood cells, that influence several immune-physiological responses. (bvsalud.org)
On admission, the patient's white blood cell count was 8000/mm3 (4,300-10,800/mm3) with a differential of 46 neutrophils, 47 bands, 3 lymphocytes, and 4 monocytes. (cdc.gov)
Blood cultures were obtained before initiation of antibiotic therapy and incubated in trypticase soy broth (1). (cdc.gov)
Blood cultures obtained on July 29 were incubated for 5 days, then discarded because they were negative. (cdc.gov)
Insect diets range from human blood, in the case of mosquitoes, to the wood used to build our houses, in the case of termites. (lu.se)
Blood culture in The patient was treated with anti- about host immunological response and monophonic and biphasic media was retroviral drugs (zidovudine, lamivu- parasite destruction when leishmaniasis negative, bone marrow aspiration and dine,nelfinavir)plusanantituberculosis is associated with immunosuppressants. (who.int)

How do you devise cell culture protocols that prevent contamination ? (corning.com)
What Are the Causes of Cell Contamination? (corning.com)
The major causes of contamination in the cell culture system are typically either chemical or biological. (corning.com)
In 3D cell culture, contamination can occur in various components, such as the bio-ink or the extracellular matrix . (corning.com)
Chemical contamination is the presence of a nonliving substance that may cause unwanted effects on the cell culture. (corning.com)
When you've discovered contamination, autoclaving is recommended to cure the contaminated culture and prevent it from spreading to other cultures. (corning.com)
Understanding cell culture contamination best practices ensures that your research quality does not have to suffer due to an unexpected occurrence, whether big or small. (corning.com)
Mycoplasma contamination of cells often goes unperceived. (invivogen.com)
Contamination happens quickly and spreads fast, so labs that rely on cell cultures must test them regularly. (invivogen.com)
This universal sensitivity, combined with an easy-to-use format, makes PlasmoTest™ a reliable, simple and cost-effective product for routine testing of all cell cultures at risk of contamination. (invivogen.com)
Contamination on a farm can send lives of a cell across the country to rich and poor. (cdc.gov)

For transposition of the gene of interest into the baculoviral DNA, we use E. coli DH10EMBacY cells. (embl.org)

A virus is a small infectious organism-much smaller than a fungus or bacterium-that must invade a living cell to reproduce (replicate). (msdmanuals.com)

a good tool to do that is the Flp-In system from Invitrogen that can be used to generate highly expressing stable cell lines. (uni-konstanz.de)

T. cruzi JAG and RF, two stains isolated from patients at IICS, maintained through successive cultured in LIT medium and the reference strain, y, were cultured in modified Grace medium (isola et. (bvsalud.org)

Spontaneous metacyclogenesis in culture of two Paraguayan T. cruzi strains has been studied. (bvsalud.org)

Autographa Californica Nuclear Polyhedrosis Virus Efficiently Enters but Does Not Replicate in Poikilothermic Vertebrate Cells. (epa.gov)
OCFVPT was isolated in the C6/36 Stegomyia albopicta (= Aedes albopictus) cell line where it replicates rapidly, but failed to replicate in Vero cells in common with other ISFs. (unl.pt)
The virus's genetic material takes control of the host cell and forces it to replicate the virus. (msdmanuals.com)

In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
Grace's Supplemented Insect Medium was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti . (fishersci.com)
In the spirit of collaboration, we have distributed Bartonella α Proteobacteria growth medium, an insect cell culture-based growth medium developed at and patented by North Carolina State University, to researchers around the world. (cdc.gov)
Subsequent studies have validated insect cell culture-based media for growth of Bartonella spp. (cdc.gov)
Nest cell culture flasks are available in cell growth areas ranging from 25cm2 to 225cm2. (thomassci.com)
Nest Multi-Layer Flasks provide 870 cm2 cell growth surface area. (thomassci.com)
Proline is Required for the Stimulation of DNA Synthesis in Hepatocyte Cultures by EGF (Epidermal Growth Factor). (epa.gov)

Most chemical contaminants are found in the cell culture media and originate from either reagents, the water used to make the reagents, or additives to supplement reagents. (corning.com)
and cell culture reagents such as bovine sera (M. arginine and A. laidlawii) or porcine trypsin (M. hyorhinis). (invivogen.com)

Cell size analysis is used to monitor SF9 cultures before and after infection. (nexcelom.com)
and these crucial immune cells are nearly wiped out within perhaps a few days of HIV infection. (treatmentactiongroup.org)

Originally derived from the Spodoptera frugiperda (fall armyworm) moth and adapted for cell culture. (nexcelom.com)

For use as a comparator with a previously published 865 Mb genome based on the LD652 pupal ovary-derived cell line, as well as whole-insect genome sequences obtained from the Asian gypsy moth subspecies L. dispar asiatica and L. dispar japonica, the whole-insect LDD genome was sequenced, assembled and annotated. (usda.gov)

After his Nobel Prize winning work elucidating the generation of B-cell and T-cell receptor diversity, Dr. Tonegawa's lab had focused on the cell and molecular biology of memory. (tritechresearch.com)

A new cloning system based on the OprI lipoprotein for the production of recombinant bacterial cell wall-derived immunogenic formulations. (unl.pt)
The work of PulseNet provides insight into the lives of a bacterial cell through DNA pattern matches. (cdc.gov)
All 3 interpretations of lives of a cell the once independent lives of a single cell, the many lives of the earth, and the lives of a bacterial cell that travels throughout the earth strongly suggest a need for multidisciplinary and interdisciplinary collaborations, such as, One Health. (cdc.gov)

Stem cell researchers value its role in maintaining pluripotency. (sigmaaldrich.com)
We have developed our own microinjectors: the analog MINJ-1, the digital MINJ-D, the high speed pneumatic MINJ-FLY, as well as the positive displacement microinjector MINJ-PD used for injecting stem cells into the brain, spinal cord, and embryos. (tritechresearch.com)

Work with one cell line at a time. (corning.com)
The Cellometer line of instruments measures the cell concentration and viability as well as the cell size. (nexcelom.com)
It usually doesn't matter because agitation intensity can be adapted to the robustness of the cell line. (genengnews.com)
However, flow cytometry data for whole-insect LDD indicates it has a genome size more than 10% larger than its cell line counterpart. (usda.gov)
Sequencing of the LDD genome was partially motivated by an interest in characterizing the content that had presumably been lost in the gypsy moth cell line during the four decades it has been propagated in culture. (usda.gov)

Obtain cell concentration, viability and cell size diameters. (nexcelom.com)
Cellometer Auto T4 images and viability analysis of uninfected and infected Sf9 cells. (nexcelom.com)

The epimastigote form of Trypanosoma cruzi is the multiplying stage of the parasite that grows in the gut of the insect vector and also in cell-free culture medium as shown here. (medscape.com)
Three Cabus apella monkeys were infected with 3.5 x 10 sobre 5 cells of Trypanosoma cruzi Y strian in order to evaluate the parasitemia and to study the behavior of the parasites in the mammalian host for a one-year period. (bvsalud.org)

Biological contaminants often enter cell cultures by use of nonsterile supplies and media. (corning.com)

Due to their minimal genome, they cannot perform metabolic functions such as production of a cell wall, or synthesis of nucleotides or amino acids. (invivogen.com)

The phrase lives of a cell refers to the independent yet interrelated parts of a human cell including mitochondria, centrioles, and basal bodies that once led independent lives. (cdc.gov)

Insect-based pet food ingredients have been growing in popularity for years, backed by sustainability and nutrition trends, while sometimes hindered by regulation. (petfoodindustry.com)
Pet food and treat makers can learn more about insect-based pet food ingredients at Petfood Forum . (petfoodindustry.com)

Anatomy20

Organisms70

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Psychiatry and Psychology3

Phenomena and Processes64

Disciplines and Occupations1

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Bacteria17

Proteins12

Protein15

Drosophila1

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Yeast2

Suspension3

Metabolism1

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Viruses6

Commonly3

Tissue culture10

Sf213

Vectors5

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Plant3

Hosts4

Species5

Adherent1

Tissues2

Routinely2

Replication1

Isolation2

Infect2

Expression4

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