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  • genome
  • Genome-wide coorientation of replication and transcription reduces adverse effects on replication in Bacillus subtilis. (semanticscholar.org)
  • The small, 195-amino-acid form of the hepatitis delta virus (HDV) antigen (δAg-S) is essential for genome replication, i.e., for the transcription, processing, and accumulation of HDV RNAs. (asm.org)
  • After transfection of these RNPs into human cells, we detected HDV genome replication, as assayed by Northern analysis or immunofluorescence microscopy. (asm.org)
  • Our interpretation is that the input δAg-S is necessary for the RNA to undergo limited amounts of RNA-directed RNA synthesis, RNA processing, and mRNA formation, leading to de novo translation of δAg-S. It is this second source of δAg-S which then goes on to support genome replication. (asm.org)
  • This assay made it possible to manipulate in vitro the composition of the RNP and then test in vivo the ability of the complex to initiate RNA-directed RNA synthesis and go on to achieve genome replication. (asm.org)
  • We expect that further in vitro modifications of these RNP complexes should help define the in vivo requirements for what we define as the initiation of HDV genome replication. (asm.org)
  • The small 195-amino-acid (aa) form, δAg-S, is essential for genome replication ( 14 ). (asm.org)
  • First, δAg-L can act as a dominant-negative inhibitor of genome replication ( 5 ). (asm.org)
  • While much valuable information can be gained about HDV genome replication by the use of cDNA transfections, there are obvious inadequacies when it comes to studying the early events of RNA-directed RNA synthesis. (asm.org)
  • Human adeno-associated virus type 2 (AAV-2) is a nonpathogenic parvovirus that requires helper functions provided by viruses such as adenovirus or herpes simplex virus for efficient replication of its genome ( 2 , 60 ). (pubmedcentralcanada.ca)
  • homologous
  • Our results indicate that multiple activities of Rad5 function coordinately with homologous recombination factors to enable replication template switch events that join sister chromatids at stalled forks in order to bypass DNA damage. (roswellpark.org)
  • sequence-specific
  • In contrast, when replicating intermediates are isolated from mammalian cells, fractionated by two-dimensional (2D) gel electrophoresis, and then hybridized with sequence-specific probes, initiation events appear to be distributed almost randomly throughout large DNA regions (initiation zones). (pubmedcentralcanada.ca)
  • function
  • In this study, I have exploited the cold sensitive growth phenotype due to loss of Hda function and its suppressors to understand the Hda function beyond initiation of replication. (buffalo.edu)
  • Our work demonstrated that all of these conditions and the cognate signals function to regulate the activation (phosphorylation) of Spo0A, the key transcription factor required for the initiation of sporulation. (mit.edu)