• The drug works by inhibiting the HCV RNA polymerase, which is essential for viral replication. (clearsynth.com)
  • HCV NS5B polymerase plays an essential role in HCV replication. (medscape.com)
  • Sofosbuvir is a NS5B polymerase inhibitor that results in suppression of HCV replication and interrupts HCV life cycle. (medscape.com)
  • Sofosbuvir is an inhibitor of HCV NS5B RNA-dependent polymerase. (medscape.com)
  • Dasabuvir is a nonnucleoside NS5B RNA-dependent polymerase inhibitor. (medscape.com)
  • Sofosbuvir in Hepcvir tablets 400mg is a direct-acting nucleotide analogue inhibitor that blocks the action of the HCV NS5B polymerase, an enzyme that is essential to viral replication. (medypharma.com)
  • It is a nucleotide prodrug that is converted to its active form, the uridine analog triphosphate (GS-461203), which is incorporated into HCV RNA by the action of NS5B polymerase. (medypharma.com)
  • Mechanistically, SOF inhibits HCV replication by restricting the activity of the ribonucleic acid (RNA)-dependent RNA polymerase (RdRp). (mybestmedicine.com)
  • Hepatitis E virus (HEV) ORF1 protein (pORF1) contains methyltransferase (MetT), papain-like cysteine protease (PCP), RNA helicase (Hel) and RNA-dependent RNA polymerase (RdRp) domains. (microbiologyresearch.org)
  • We are currently investigating a) replicative enzymes: helicases and DNA polymerase b) transcriptional enzymes: DNA-dependent RNA polymerase. (rutgers.edu)
  • and c) viral replicases: HCV RNA helicase and RNA polymerase. (rutgers.edu)
  • T7 RNA polymerase is one of the best structurally characterized proteins of this class. (rutgers.edu)
  • In 2013, Gilead received FDA approval for a new HCV drug, Sovaldi (sofosbuvir), that inhibits viral replication by targeting the virus's NS5B polymerase. (michaelchimenti.com)
  • For hepatitis C virus (HCV) genotype specific activity, see sections 4.4 and 5.1. (medicines.org.uk)
  • For previously treated patients with HCV genotype 1 infection, no data exists with the combination of Sovaldi, ribavirin and peginterferon alfa (see section 4.4). (medicines.org.uk)
  • The effect was independent of the HCV genotype and both infection of cells by extra-cellular virions and cell-to-cell spread were blocked. (activeboard.com)
  • The backbone of treatment for chronic hepatitis C virus (HCV) infection regardless of genotype was combination therapy with injectable pegylated interferon-alfa (PEG-IFNa) plus oral ribavirin. (medscape.com)
  • In a study of treatment-naïve US veterans with genotype 1 HCV, ombitasvir-based therapy was considered to be the most economically efficient strategy. (medscape.com)
  • The specific combination therapy regimen for HCV infection depends on the HCV genotype and the degree of liver damage due to the infection. (medypharma.com)
  • Daklinza is indicated for use with sofosbuvir, with or without ribavirin, for the treatment of patients with chronic hepatitis C virus (HCV) genotype 1 or genotype 3 infections. (selectshopedpills.com)
  • We studied a 56-year-old treatment-naïve Japanese man with chronic HCV genotype 2b infection who had ulcerative colitis. (biomedcentral.com)
  • We report an ulcerative colitis-patient who was chronically infected with HCV genotype 2 and was successfully treated with sofosbuvir plus dose reduction of ribavirin for 12 weeks. (biomedcentral.com)
  • The HCV genotype was 2b, and the patient was interferon treatment-naïve because he had ulcerative colitis, an autoimmune disease. (biomedcentral.com)
  • PSI-353661, a phosphoramidate prodrug of 2'-deoxy-2'-fluoro-2'-C-methylguanosine-5'-monophosphate, is a highly active inhibitor of genotype 1a, 1b, and 2a HCV RNA replication in the replicon assay and of genotype 1a and 2a infectious virus replication. (inrae.fr)
  • Conclusions: Turmeric curcumin inhibits HCV entry independently of the genotype and in primary human hepatocytes by affecting membrane fluidity thereby impairing virus binding and fusion. (princeton.edu)
  • Pediatric Patients: The pharmacokinetics of sofosbuvir, GS-331007, and velpatasvir were determined in HCV genotype 1, 2, 3, 4, or infected pediatric subjects years of age and older receiving daily dose of EPCLUSA as described below in Table 7. (drugcentral.org)
  • Daklinza (daclatasvir) is a hepatitis C virus (HCV) NS5A inhibitor indicated for use with sofosbuvir for the treatment of chronic HCV genotype 3. (rxlist.com)
  • Sustained virologic response (SVR12) rates are reduced in HCV genotype 3-infected patients with cirrhosis receiving DAKLINZA in combination with sofosbuvir for 12 weeks [see Clinical Studies ]. (rxlist.com)
  • Consider screening for the presence of NS5A polymorphisms at amino acid positions M28, Q30, L31, and Y93 in patients with cirrhosis who are infected with HCV genotype 1a prior to the initiation of treatment with DAKLINZA and sofosbuvir with or without ribavirin [see Microbiology , Table 11]. (rxlist.com)
  • Table 1 provides the recommended DAKLINZA-containing treatment regimens and duration based on HCV genotype and patient population. (rxlist.com)
  • The optimal duration of DAKLINZA and sofosbuvir with or without ribavirin has not been established for HCV genotype 3 patients with cirrhosis or for HCV genotype 1 patients with Child-Pugh C cirrhosis [see Clinical Studies ]. (rxlist.com)
  • It has been shown that the JFH1 and the chimeric J6/JFH1 isolate of the 2a genotype of HCV replicate efficiently in Huh7 cells [4,5] and in the highly permissive Huh7.5 and Huh7.5.1 cells derived from the human hepatoma cell line Huh7 [6-9]. (virology.com.cn)
  • Egypt has the compared HCVcAg with HCV RNA and can lead to increased HCV mor- highest prevalence of HCV infection in genotype 4 infected patients ( 9-11 ). (who.int)
  • 1 ). The disease causes chronic hepatic HCV genotype 4 prevails ( 12 ) and ( 13,14 ). (who.int)
  • Statistical analysis can be used, namely total anti-HCV This study was conducted from De- All data were entered into a Microsoft antibodies, HCV-RNA levels, HCV cember 2012 to March 2013 at the Excel worksheet and statistical analysis genotype and recently HCV core anti- Viral Hepatitis Research Laboratory at was performed using SPSS for Win- gen (HCVcAg) levels ( 5 ). (who.int)
  • Sofosbuvir is a direct-acting antiviral medication used in the treatment of chronic hepatitis C virus (HCV) infection. (clearsynth.com)
  • Overall, the use of GS-566500 13CD3 is an important tool in the development and optimization of Sofosbuvir therapy for patients with HCV infection. (clearsynth.com)
  • It is indicated for use with sofosbuvir for chronic HCV genotypes 1 and 3 infection. (medscape.com)
  • Hepcvir tablets 400mg contain sofosbuvir, a direct-acting antiviral agent that is used in combination with other antiviral medications to treat chronic HCV infection. (medypharma.com)
  • This action of sofosbuvir in Hepcvir L tablets causes chain termination of viral RNA synthesis thereby inhibiting viral replication. (medypharma.com)
  • Sofosbuvir in Hepcvir tablets is an effective treatment for HCV infection only when used in combination with at least one other antiviral medication . (medypharma.com)
  • Postmarketing cases of symptomatic bradycardia and cases requiring pacemaker intervention have been reported when amiodarone is coadministered with sofosbuvir in combination with another HCV direct-acting antiviral, including Daklinza. (selectshopedpills.com)
  • Sofosbuvir (SOF) is an antiviral drug that has received approval from the FDA for hepatitis C (HCV) treatment. (mybestmedicine.com)
  • Clinicians should pay careful attention to the ribavirin dose in the treatment of certain HCV patients with inflammatory bowel disease who are receiving sofosbuvir plus ribavirin. (biomedcentral.com)
  • NA not applicable.Cmax (ng/mL)567 (30.7)898 (26.7)259 (54.3)AUCtau (nghr/mL)1268 (38.5)14372 (28.0)2980 (51.3)Ctrough (ng/mL)NA-42 (67.3)Sofosbuvir and GS-331007 AUC0-24 and Cmax were similar in healthy adult subjects and subjects with HCV infection. (drugcentral.org)
  • However, velpatasvir exhibited more than or near dose-proportional increase in exposures 25 mg to 150 mg in HCV-infected patients when coadministered with sofosbuvir. (drugcentral.org)
  • NS5A not only binds to cellular membranes, other non-structural proteins of the HCV replication-complex and viral RNA, but also to co-factors like PI4KA. (wikipedia.org)
  • Non-structural proteins are involved in the transcription and replication of the virus. (medsci.org)
  • Current direct-acting antiviral (DAA)-based therapies that target viral proteins involving HCV genome replication are effective, however a minority of patients still fail to cure HCV, rendering a window to develop additional antivirals particularly targeting host functions involving in HCV infection. (bvsalud.org)
  • Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. (thermofisher.com)
  • Drug development is focused on non-structural proteins required for RNA replication. (activeboard.com)
  • Glecaprevir is an inhibitor of the HCV NS3/4A protease, which is a viral enzyme necessary for the proteolytic cleavage of the HCV-encoded polyprotein into mature forms of the NS3, NS4A, NS4B, NS5A, and NS5B proteins. (saifpharma.com)
  • These multifunctional proteins, including NS3, are essential for viral replication. (saifpharma.com)
  • NS5A is a phosphoprotein that plays an essential role in the replication, assembly, and maturation of infectious viral proteins. (saifpharma.com)
  • By blocking this interaction, pibrentasvir inhibits the assembly of proteins and the production of mature HCV particles. (saifpharma.com)
  • We are studying the mechanism of NS3 as well as its interactions with other HCV and cellular proteins. (uams.edu)
  • Our goal is to recapitulate RNA replication in vitro using biological relevant substrates and proteins and a biologically relevant sub-genomic replicon of HCV. (uams.edu)
  • The replication of a virus depends on the delivery of its genome to a host cell, which subsequently produces viral proteins using the host cell. (creative-biolabs.com)
  • 5. HCV Genome: Polyprotein HCV contains a positive sense RNA genome of approximately 9600 bases, codes for a single polyprotein precursor of about 3000 amino acides that is co- and posttranslationally cleaved into structural and nonstructural proteins. (slideserve.com)
  • TGF-β induction by viral proteins is one of the ways to escape the virus from the immune system by inhibiting interferon signaling and other immune system factors. (org.ir)
  • Soon thereafter, it was found that the efficiency of replicon RNA amplification was determined by cell culture adaptive mutations within the viral proteins and by selection for particular cells that are highly permissive (3). (virology.com.cn)
  • While all coronaviruses contains these proteins, a subset (HEV, HCV-OC43 and BCV) is now recognized to possess an additional glycopolypeptide (gp65), which is unrelated to S or M. N protein The number of amino acids in the N protein has been determined by cloning and sequencing for MHV strains A59 (Armstrong et al. (pdfroom.com)
  • Helicases are ubiquitous proteins that catalyze the separation of duplex DNA strands, the removal of secondary structures in RNA, and the reorganization of proteins bound to DNA. (rutgers.edu)
  • Being involved in almost all DNA and RNA metabolism processes, helicases and related proteins constitute greater than 2% of the genome. (rutgers.edu)
  • Biochemical studies reveal that helicases are nucleic acid motor proteins that use the chemical energy from NTP hydrolysis to move along DNA and RNA. (rutgers.edu)
  • We study the HCV proteins including the helicase and replicase to understand their function and mechanism of action, substrate specificity, and structure-function-studies to ultimately aid in obtaining agents to inhibit their function. (rutgers.edu)
  • In this study, we screened all the nonstructural proteins of HCV and found that HCV NS2 could suppress RNAi induced either by small hairpin RNAs (shRNAs) or small interfering RNAs (siRNAs) in mammalian cells. (virosin.org)
  • Fabozzi G, Nabel CS, Dolan MA, Sullivan NJ (2011) Ebolavirus proteins suppress the effects of small interfering RNA by direct interaction with the mammalian RNA interference pathway. (virosin.org)
  • Ji J, Glaser A, Wernli M, Berke JM, Moradpour D, Erb P (2008) Suppression of short interfering RNA-mediated gene silencing by the structural proteins of hepatitis C virus. (virosin.org)
  • During many stages of the viral replication cycle in the cytoplasm, + RNA viruses interact with host proteins and alter cell homeostasis to benefit viral replication and assembly [ 12 , 13 ]. (biomedcentral.com)
  • Bepirovirsen is a potential first-in-class antisense oligonucleotide that targets all HBV messenger RNA and acts to decrease levels of viral proteins. (medscape.com)
  • RNA, lipids, and proteins. (who.int)
  • A substantial number of small molecule compounds have been reported as potent inhibitors of the replication of picornaviruses in vitro ( 8 ). (cdc.gov)
  • We report on a comparative study of a selected series of antipicornavirus drugs for their ability to inhibit PV replication in vitro. (cdc.gov)
  • A phosphoramidate prodrug of 2'-deoxy-2'-alpha-fluoro-beta-C-methyluridine-5'-monophosphate, PSI-7851, demonstrates potent anti-hepatitis C virus (HCV) activity both in vitro and in vivo. (inrae.fr)
  • Gamma interferon (IFN-γ) offers been proven to inhibit replication of subgenomic and genomic hepatitis C pathogen (HCV) RNAs in vitro and to noncytolytically suppress hepatitis B computer virus (HBV) replication in vivo. (researchtoactionforum.org)
  • It was with the discovery of the replicon system in 1999 that for the first time HCV RNA replication could be reproduced in vitro in Huh-7 human hepatocellular carcinoma cell lines. (slideserve.com)
  • And for the first time in 2005 JFH-1 genomes transfected into Huh-7 cells produced infectious virus allowing in vitro study of the entire HCV life cycle. (slideserve.com)
  • The ability to grow HCV in vitro is important for understanding both virologic and immunologic aspects of HCV infections. (virology.com.cn)
  • In vitro, several statins have shown potent antiviral activity in cells harboring HCV replicon, but lower effect were obtained in cell infected with HCVcc (Delang et al. (cp-673451inhibitor.com)
  • However the divergence between various statins in their ability to inhibit HCV in vitro is still not understood, emphasizing the complex interrelation of HCV lifecycle and lipids metabolism. (cp-673451inhibitor.com)
  • Together, our findings uncovered that HCV NS2 can act as a VSR in vitro, thereby providing novel insights into the life cycle and virus-host interactions of HCV. (virosin.org)
  • Pharmacological inhibition of DGAT-1 decreased LD accumulation and ZIKV replication in vitro in human cells and in an in vivo mouse model of infection. (biomedcentral.com)
  • In this study, we identified the polyphenol epigallocatechin-3-gallate (EGCG) as an inhibitor of HCV entry. (activeboard.com)
  • RDV is taken up in the target cells and metabolized in multiple steps to form the active nucleoside triphosphate (TP) (GS-443902), which in turn acts as a potent and selective inhibitor of multiple viral RNA polymerases. (inrae.fr)
  • The aim of the study was to determine the potency of turmeric curcumin as an HCV entry inhibitor. (princeton.edu)
  • DAKLINZA (daclatasvir) is an inhibitor of HCV nonstructural protein 5A (NS5A). (rxlist.com)
  • favipiravir) is a broad spectrum RNA replicase inhibitor, used to treat Influenza. (sathguru.com)
  • We compared the HCV anti- viral effect of the most potent SKI-1/S1P small molecule inhibitor (PF-429242) with a set of two statins on different steps of the viral life cycle, and showed that SKI-1/S1P inhibitor blocked HCVcc (strain JFH-1) RNA replication (EC50 = 5.8 lM) more efficiently than statins. (cp-673451inhibitor.com)
  • What is interesting about Sovaldi is the approach the scientists took to getting the inhibitor into the cell, relying on phosphoramidate prodrug technology that had been effectively used to develop anti-HIV drugs, but had never been applied before to this class of anti-HCV drugs. (michaelchimenti.com)
  • We here report on a comparative study of the antipoliovirus activity of a selection of molecules that have previously been reported to be inhibitors of picornavirus replication and discuss their potential use, alone or in combination, for the treatment or prophylaxis of poliovirus infection. (cdc.gov)
  • Sovaldi oral granules are available for the treatment of chronic HCV-infection in paediatric patients aged 3 years and above having difficulty in swallowing film-coated tablets. (medicines.org.uk)
  • Hepatitis C virus (HCV) infection causes severe liver diseases and remains a major global public health concern. (bvsalud.org)
  • Here, we utilized the HCV infection cell culture system (HCVcc) to screen in-house compounds bearing host-interacting preferred scaffold for the antiviral activity. (bvsalud.org)
  • Therefore, antiviral strategies targeting the early stages of infection are urgently needed for the prevention of HCV infection. (activeboard.com)
  • Pretreatment of cells with EGCG before HCV inoculation did not reduce HCV infection while application of EGCG during inoculation strongly inhibited HCV infectivity. (activeboard.com)
  • It is indicated for the treatment of CHC infection genotypes 1, 2, 3, and 4 as part of a combination antiviral regimen, including those with hepatocellular carcinoma meeting Milan criteria (awaiting liver transplantation) to prevent HCV recurrence and those with HCV/HIV-1 coinfection. (medscape.com)
  • These include interferon, which is a chemical messenger produced by host cells in response to infection by various pathogens, including viruses that stimulates the body's own immune response, as well as directly inhibiting viral replication in infected cells. (medypharma.com)
  • The combination of Hepcvir tablets 400 mg and ribavirin or ribavirin with interferon lowers the amount of hepatitis C virus in the blood and may also lead to a cure of HCV infection over a number of weeks. (medypharma.com)
  • Direct-acting antivirals (DAAs) are now available for the treatment of chronic hepatitis C virus (HCV) infection. (biomedcentral.com)
  • A 56-year-old man with a 31-year history of ulcerative colitis was diagnosed with HCV infection at age 46. (biomedcentral.com)
  • The patient received a blood transfusion at age 4 when he had surgery for his jaw and denied other risk factors for HCV infection, including tattoos or intravenous drug use. (biomedcentral.com)
  • HCV) infection. (magicinepharma.com)
  • Objective: Hepatitis C virus (HCV) infection causes severe liver disease and affects more than 160 million individuals worldwide. (princeton.edu)
  • Test all patients for evidence of current or prior HBV infection by measuring hepatitis B surface antigen (HBsAg) and hepatitis B core antibody (anti-HBc) before initiating HCV treatment with DAKLINZA [see WARNINGS AND PRECAUTIONS ]. (rxlist.com)
  • Several clinical observations point to an intricate crosstalk between iron (Fe) metabolism and chronic hepatitis C virus (HCV) infection. (microbiologyresearch.org)
  • These data may therefore contribute to a better understanding of the complex interplay between HCV and cellular physiology during infection. (microbiologyresearch.org)
  • Numerous examples in the literature document that infection of primary human liver cells and several cell lines with serum-derived HCV is possible and that the viral genome can be kept in some of these cell lines for up to 2 years (reviewed in ref. 3). (virology.com.cn)
  • Immune globulin and antiviral agents (e.g., interferon with or without ribavirin) are not recommended for PEP of hepatitis C. For HCV postexposure management, the HCV status of the source and the exposed person should be determined, and for HCP exposed to an HCV positive source, follow-up HCV testing should be performed to determine if infection develops. (cdc.gov)
  • World- wide, approximately 170 million patients are chronically infected, and it has been estimated that over 300,000 individuals die annu- ally from HCV infection. (cp-673451inhibitor.com)
  • Moreover, we observed that inhibition of DGAT-1 inhibited the weight loss and mortality induced by ZIKV infection in vivo. (biomedcentral.com)
  • Our results reveal that LD biogenesis triggered by ZIKV infection is a crucial step for ZIKV replication and pathogenesis in neural cells. (biomedcentral.com)
  • Our results confirm a potential role of NK cells and the different subsets in the pathogenesis of chronic HCV infection. (who.int)
  • et 20 témoins en bonne santé ne présentant pas d'infection par le virus de l'hépatite C. Une réduction importante de la fréquence des cellules tueuses naturelles totales dans le groupe des patients porteurs d'une infection chronique a été observée par rapport au groupe des témoins ( P = 0,001) ou au groupe des patients dont l'infection a connu une résolution spontanée ( P = 0,01). (who.int)
  • Hepatitis C virus (HCV) infection has impaired cellular immune response and circulating NK cells, NK subsets (in- the ability to establish chronic infection virus persistence [8]. (who.int)
  • ABSTRACT Hepatitis C virus (HCV) infection is widespread in Egypt. (who.int)
  • HCV-RNA remains the gold standard for diagnosis of active HCV infection but HCVcAg can be used where PCR is not available. (who.int)
  • Hepatitis C virus (HCV) infection is expertise to perform. (who.int)
  • 2 ). HCV infection is often further pare HCV-RNA quantification with The institutional review board of the complicated by underlying hepatitis B HCVcAg presence and quantification National Hepatology and Tropical and Shistosoma mansoni co-infection. (who.int)
  • Co-infection leads to higher morbidity HCV-infected Egyptians. (who.int)
  • Virological diagnosis and monitor- consent form prior to participating in ing of HCV infection are based on the Methods the study. (who.int)
  • It works by inhibiting the HCV protein NS5A. (wikipedia.org)
  • NS5A is a zinc-binding protein dimer serving as a regulator in the replication cycle of HCV. (wikipedia.org)
  • Domain 1 (amino acid 401-60012) is an RNA binding region of NS5A and domain 2 serves in the RNA replication. (wikipedia.org)
  • NS5A is integral for HCV RNA viral replication. (medscape.com)
  • Daclatasvir inhibits NS5A, a nonstructural protein encoded by HCV. (medscape.com)
  • It binds to the N-terminus within domain 1 of NS5A, which may cause structural distortions that interfere with NS5A functions, and thereby inhibits both viral RNA replication and virion assembly. (medscape.com)
  • Ledipasvir inhibits HCV NS5A protein, which is required for viral replication. (medscape.com)
  • Daclatasvir is used to inhibit NS5A protein that is viral specific and is vital for the replication of HCV RNA. (actizapharmaceutical.com)
  • Daclatasvir inhibits the HCV nonstructural protein NS5A. (selectshopedpills.com)
  • NS5A) and causes a decrease in serum HCV RNA levels. (magicinepharma.com)
  • Daclatasvir, sold under the brand name Daklinza, is an antiviral medication used in combination with other medications to treat hepatitis C (HCV). (wikipedia.org)
  • Daklinza (daclatasvir) is an antiviral medicine that prevents hepatitis C virus (HCV) from multiplying in your body. (selectshopedpills.com)
  • Notably, this drug can also inhibit other single-stranded viruses, including coronaviruses. (mybestmedicine.com)
  • Ribozymes are single-stranded RNA molecules that specifically inhibit viral gene expression and replication. (creative-biolabs.com)
  • Nucleic acid aptamers are single-stranded oligonucleotides (RNA or DNA) 30 to 100 nt long that can bind to and affect the activity of target molecules with high specificity and affinity. (creative-biolabs.com)
  • Hepatitis C virus (HCV) is a single-stranded RNA virus that infects an estimated 180 million people worldwide. (michaelchimenti.com)
  • Positive-sense RNA viruses possess a single-stranded RNA genome that can serve as messenger RNA (mRNA) that can be directly translated to produce an amino acid sequence. (msdmanuals.com)
  • Negative-sense RNA viruses possess a single-stranded negative-sense genome that first must synthesize a complementary positive-sense antigenome, which is then used to make genomic negative-sense RNA. (msdmanuals.com)
  • The N-terminal of NS3 protein confers serine protease activity, while the C-terminus of NS3 encodes a DExH/D-box RNA helicase which hydrolyzes NTP as an energy source to unwind double-stranded RNA in a 3′ to 5′ direction during replication of viral genomic RNA. (saifpharma.com)
  • NS3 is an RNA helicase that is capable of unwinding DNA. (uams.edu)
  • However, it potently inhibited HCVcc entry into hepatoma cell lines as well as primary human hepatocytes. (activeboard.com)
  • Conclusion: the green tea molecule EGCG potently inhibits HCV entry and could be part of an antiviral strategy aimed at the prevention of HCV reinfection after liver transplantation. (activeboard.com)
  • However, co-incubation of HCV with curcumin potently inhibited entry of all major HCV genotypes. (princeton.edu)
  • Intriguingly, HIV-lhNef potently inhibited wild-type HIV-1 production in trans. (biomedcentral.com)
  • Nevertheless, the highest inhibition of SARS-CoV-2 replication, without inducing cell death, occurred at 20 μM SOF concentration. (mybestmedicine.com)
  • RNA interference (RNAi) is a naturally occurring gene regulatory process to achieve gene silencing through chromatin remodeling, inhibition of protein translation, or direct mRNA degradation. (creative-biolabs.com)
  • Broad-range inhibition of enterovirus replication by OSW-1, a natural compound targeting OSBP. (microbiologyresearch.org)
  • Inhibition of cellular proteasome activities mediates HBX-independent hepatitis B virus replication in vivo J Virol. (usc.edu)
  • Pharmacological interference in lipid metabolism and LD inhibition affects viral replication of different viruses. (biomedcentral.com)
  • The phosphoramidate prodrug technology had never been applied to HCV inhibition until Solvadi. (michaelchimenti.com)
  • Replicon clearance studies show that PSI-353661 was able to clear cells of HCV replicon RNA and prevent a rebound in replicon RNA. (inrae.fr)
  • The second great discovery came 2003 when an HCV GT 2a clone isolated from a Japanese patient with a rare case of fulminant HCV designated JFH-1 was found to replicate in Huh-7 and other cell lines without the need for adaptive mutations as prior replicon clones. (slideserve.com)
  • A first major obstacle toward an efficient cell culture system was overcome with the invention of the HCV replicon system (4). (virology.com.cn)
  • The latter conclusion is based on the observation that removal of the replicon from a cell clone by treatment with IFN or a selective drug frequently results in cell clones that support higher levels of HCV RNA replication as compared to na?ve Huh-7 cells. (virology.com.cn)
  • There are three pathways capable of producing double-stranded RNA (dsRNA) molecules that activate RNAi, including microRNA (miRNA), short interfering RNA (siRNA), and short hairpin RNA (shRNA). (creative-biolabs.com)
  • The underlying reason for the higher permissiveness is largely unknown, but for one particular cell clone, designated Huh7.5 (5), a single point mutation in the dsRNA sensor retinoic acid-inducible gene-I (RIG-I) was found to be involved in higher permissiveness for HCV RNA replication (6). (virology.com.cn)
  • Activation of RIG-I by dsRNA, such as HCV RNA, results in the phosphorylation and nuclear translocation of IFN regulatory factor-3 (IRF-3), activating innate antiviral defenses. (virology.com.cn)
  • This defect, together with the overall very low expression of the exogenous dsRNA sensor Toll-like receptor 3 in Huh-7 cells (7), could explain why HCV replicates so efficiently in Huh7.5 cells . (virology.com.cn)
  • The presence of the 'kissing loop' interaction inhibited the formation of SL9571 in the 3'-X-tail, an RNA structure implicated in genome replication. (ox.ac.uk)
  • The research in my laboratory is focused on understanding the functions and mechanisms of enzymes involved in genome replication and transcription. (rutgers.edu)
  • In both genomes, there was evidence for interaction of the sub-terminal bulge loop of SL9266 and sequences around nucleotide 9110, though the replication phenotype of genomes bearing mutations that disrupted this interaction was fundamentally different. (ox.ac.uk)
  • Paradoxes of the replication of picornaviral genomes. (microbiologyresearch.org)
  • Nevertheless, in all cases, HCV replication did not exceed copy numbers of 0.01-0.1 RNA genomes per cell, limiting the usefulness of these systems. (virology.com.cn)
  • However, only when virus production from cloned genomes is possible, the full power of reverse genetics, in which distinct mutations introduced into the viral genome can be analyzed for their impact on replication and virus production, can be used. (virology.com.cn)
  • Glecaprevir disrupts the intracellular processes of the viral life cycle by inhibiting the NS3/4A protease activity of cleaving downstream junctions of HCV polypeptide and proteolytic processing of mature structural protein. (saifpharma.com)
  • These agents interfere with HCV replication by inhibiting a key viral enzyme, NS3/4A serine protease. (medscape.com)
  • Simeprevir inhibits HCV NS3/4A protease needed for proteolytic cleavage of the HCV-encoded polyprotein into mature forms. (medscape.com)
  • NS3/4A protease is needed for proteolytic cleavage of the HCV-encoded polyprotein into mature forms. (medscape.com)
  • NS3-4A is a serine protease and associated co-factor which is involved in postranslational processing, and small peptides derived from the N-terminal cleavage products of its substrate have been shown to competitively inhibit the protease and thus has emerged as a primary target for directed antivirals known as protease inhibitors. (slideserve.com)
  • The recent addition of HCV protease inhib- itors to the standard treatment consisting of pegylated interferon- a (PEG-IFN) and ribavirin has significantly increased viral clear- ance in patients (Hezode et al. (cp-673451inhibitor.com)
  • Design: The antiviral activity of curcumin and its derivatives was evaluated using HCV pseudo-particles (HCVpp) and cell-culture-derived HCV (HCVcc) in hepatoma cell lines and primary human hepatocytes. (princeton.edu)
  • According to Watson-Crick base pairing or Hoogsteen base pairing, ASO can bind to the transcribed messenger RNA (mRNA) of the target gene and then modulate gene expression. (creative-biolabs.com)
  • The relative expression levels of TGF-β, IFN-α and HCV Core mRNA were determined using with REAL TIME PCR. (org.ir)
  • Furthermore, the efficacy of SOF was validated by analyzing intracellular viral RNA and the number of viable viruses present in the supernatants of SARS-CoV-2-infected BCOs treated with SOF. (mybestmedicine.com)
  • RNA viruses rewire cellular pathways to build replication organelles. (microbiologyresearch.org)
  • We are developing therapeutics that inhibit the viral replication function of RNA viruses that cause acute and chronic diseases. (itbusinessnet.com)
  • Host lipid metabolism, including lipid droplet (LD) biogenesis, has been associated with viral replication and pathogenesis of different viruses. (biomedcentral.com)
  • In this context, multiple + RNA viruses use the host lipid machinery to facilitate their replication and assembly [ 23 ]. (biomedcentral.com)
  • Some viruses have an outer envelope consisting of protein and lipid, surrounding a protein capsid complex with genomic RNA or DNA and sometimes enzymes needed for the first steps of viral replication. (msdmanuals.com)
  • Classification of viruses is principally according to their genome sequence taking into consideration nature and structure of their genome and their method of replication, but not according to the diseases they cause (see International Committee on Taxonomy of Viruses (ICTV), 2021 release ). (msdmanuals.com)
  • either DNA or RNA viruses may have single or double strands of genetic material. (msdmanuals.com)
  • Single-strand RNA viruses are further divided into those with (+) sense and (-) sense RNA. (msdmanuals.com)
  • DNA viruses typically replicate in the host cell nucleus, and RNA viruses typically replicate in the cytoplasm. (msdmanuals.com)
  • Certain single-strand, (+) sense RNA viruses termed retroviruses use a very different method of replication. (msdmanuals.com)
  • Because RNA transcription does not involve the same error-checking mechanisms as DNA transcription, RNA viruses, particularly retroviruses, are particularly prone to mutation. (msdmanuals.com)
  • the genome of RNA viruses ranges from 3.5 kilobases (some retroviruses) to 27 kilobases (some reoviruses), and the genome of DNA viruses ranges from 5 kilobases (some parvoviruses) to 280 kilobases (some poxviruses). (msdmanuals.com)
  • A 2019 study conducted in Puerto Rico showed that the 95th percentile for the time until the loss of Zika virus RNA detection was 54 days in serum, 34 days in urine, and 81 days in semen. (medscape.com)
  • HA410 trade name] is indicated for the treatment of chronic hepatitis B in adults with: · compensated liver disease and evidence of immune active disease, i.e. active viral replication, persistently elevated serum alanine aminotransferase (ALT) levels and histological evidence of active inflammation and/or fibrosis. (who.int)
  • Mylan Laboratories Ltd.), HA410 · compensated liver disease and evidence of immune active disease, i.e. active viral replication, persistently elevated serum ALT levels and histological evidence of active inflammation and/or fibrosis. (who.int)
  • 8 It has an envelope and contains a very large RNA virus genome. (medsci.org)
  • Its viral RNA substrates include: hepatitis C virus (HCV), vesicular stomatitis virus (VSV), measles virus (MV), hepatitis delta virus (HDV), and human immunodeficiency virus type 1 (HIV-1). (thermofisher.com)
  • Report Green Tea and HCV by mikesimon , 1 hour ago Tags: green tea, HCV The green tea polyphenol epigallocatechin-3-gallate (EGCG) inhibits hepatitis C virus (HCV) entry. (activeboard.com)
  • Abstract Hepatitis C virus (HCV) is a major cause of liver cirrhosis and hepatocellular carcinoma. (activeboard.com)
  • Recent advances in the treatment of patients infected with HCV make it possible to eradicate this virus with interferon-free regimens. (biomedcentral.com)
  • The RNA structure and long-range interactions of the SL9266 cis-acting replication element located within the NS5B coding region of hepatitis C virus (HCV) were determined using selective 2'-hydroxyl acylation analysed by primer extension. (ox.ac.uk)
  • Epclusa is a prescription brand-name medication that's used to treat hepatitis C virus (HCV) in adults. (fixhepc.com)
  • Expression levels of known HCV receptors were unaltered, while pretreating the virus with the compound reduced viral infectivity without viral lysis. (princeton.edu)
  • Currently, a series of ribozymes have been developed against human papillomaviruses (HPV), acquired immunodeficiency syndrome (AIDS), and the hepatitis C virus (HCV). (creative-biolabs.com)
  • For the treatment of severe acute respiratory syndrome (SARS), a chimeric DNA-RNA hammerhead ribozyme has been developed to significantly inhibit the proliferation of mouse hepatitis virus (MHV) in DBT cells. (creative-biolabs.com)
  • Here we investigated the role of PI4KIII in foot-and-mouth disease virus (FMDV) replication. (microbiologyresearch.org)
  • Cholesterol shuttling is important for RNA replication of coxsackievirus B3 and encephalomyocarditis virus. (microbiologyresearch.org)
  • Our earlier study has demonstrated that hepatitis C virus (HCV)-associated cholesterol plays a key role in virus infectivity. (microbiologyresearch.org)
  • Human immunodeficiency virus type 1 (HIV-1) can be inhibited by means of RNA silencing or interference (RNAi) using synthetic short interfering RNAs (siRNAs) or gene constructs encoding short hairpin RNAs (shRNAs) or long hairpin RNAs (lhRNAs). (biomedcentral.com)
  • However, HIV-lhNef demonstrated a severe production and replication defect, which we were able to solve by selecting spontaneous virus variants with truncated hairpin structures. (biomedcentral.com)
  • Although these escape variants lost the ability to trans-inhibit HIV-1, they effectively outgrew the wild-type virus in competition experiments in SupT1 cells. (biomedcentral.com)
  • Binds RNA and may function as a RNA chaperone to induce the RNA structural rearrangements taking place during virus replication (PubMed:18033802). (proteopedia.org)
  • 6 Blight KJ, McKeating JA, Rice CM: Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication. (virology.com.cn)
  • Complete replication of hepatitis C virus in cell culture. (virology.com.cn)
  • Viral-load-dependent effects of liver injury and regeneration on hepatitis B virus replication in mice J Virol. (usc.edu)
  • Replication of hepatitis C virus RNA on autophagosomal membranes J Biol Chem. (usc.edu)
  • Enhancement of hepatitis B virus replication by androgen and its receptor in mice J Virol. (usc.edu)
  • Hepatitis C virus translation preferentially depends on active RNA replication PLoS One. (usc.edu)
  • Autophagy required for hepatitis B virus replication in transgenic mice J Virol. (usc.edu)
  • Krüppel-like factor 15 activates hepatitis B virus gene expression and replication Hepatology. (usc.edu)
  • Hepatitis C virus inhibits DNA damage repair through reactive oxygen and nitrogen species and by interfering with the ATM-NBS1/Mre11/Rad50 DNA repair pathway in monocytes and hepatocytes J Immunol. (usc.edu)
  • B virus (HBV), hepatitis C virus (HCV), or human immunodeficiency virus (HIV). (cdc.gov)
  • Hepatitis C virus (HCV) is often associated with steatosis, cirrhosis and hepatocellular carcinoma (HCC). (cp-673451inhibitor.com)
  • The hepatitis C virus (HCV) is known to be a prominent factor for the onset of end-stage liver diseases such as liver cirrhosis and hepatocellular carcinoma (HCC) (Di Bisceglie, 1997). (cp-673451inhibitor.com)
  • Additional representatives of the family reviewed in this article are the human (HCV) and bovine (BCV) coronaviruses, transmissible gastroenteritis virus (TGEV), haemagglutinating encephalitis virus (HEV) and feline infectious peritonitis virus (FIPV). (pdfroom.com)
  • Hepatitis C virus (HCV) is a medically important human pathogen that causes acute and chronic hepatitis. (virosin.org)
  • Haasnoot J, de Vries W, Geutjes EJ, Prins M, de Haan P, Berkhout B (2007) The Ebola virus VP35 protein is a suppressor of RNA silencing. (virosin.org)
  • Kakumani PK, Ponia SS, Sood V, Chinnappan M, Banerjea AC, Medigeshi GR, Malhotra P, Mukherjee SK, Bhatnagar RK (2013) Role of RNA interference (RNAi) in dengue virus replication and identification of NS4B as an RNAi suppressor. (virosin.org)
  • By designing and selecting antiviral drug candidates that interrupt the viral replication process and have specific binding characteristics, we seek to develop drugs that are effective against both the virus and mutants of the virus, and also have reduced off-target interactions that may cause undesirable clinical side effects. (itbusinessnet.com)
  • Blood and any body fluid visibly contaminated with blood should be considered capable of transmitting hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV). (medscape.com)
  • Few participants had detectable Zika virus RNA in saliva or vaginal secretions. (medscape.com)
  • Studies sug- terized by flow cytometry using anti between the immune response and the gest that HCV inhibits receptor genes in CD3, CD56 and CD16 monoclonal virus replication rate [5] and play a cru- the activation of NK cells [20], and the antibodies. (who.int)
  • RÉSUMÉ L'infection par le virus de l'hépatite C (VHC) est répandue en Égypte. (who.int)
  • As a double-stranded molecule, one strand consists entirely of DNA and the other consists of two 10 nt 2'-O-methyl (2'-O-Me) RNA. (creative-biolabs.com)
  • An alternative method to inhibit HIV-1 is the use of gene constructs encoding HIV-1-specific long hairpin RNAs (lhRNAs, transcripts folding an extended hairpin structure) or long double-stranded RNAs (dsRNAs, two complementary transcripts that form an extended duplex). (biomedcentral.com)
  • Moreover, we demonstrated that NS2 could suppress RNAi via its direct interaction with double-stranded RNAs (dsRNAs) and siRNAs, and further identified that the cysteine 184 of NS2 is required for the RNAi suppression activity through a serial of point mutation analyses. (virosin.org)
  • Retroviruses use reverse transcription to create a double-stranded DNA copy (a provirus) of their RNA genome, which is inserted into the genome of their host cell. (msdmanuals.com)
  • In keeping with previous observations we confirmed that supra-physiological intracellular Fe induced by haemin treatment down-modulated HCV replication from subgenomic replicons. (microbiologyresearch.org)
  • Out of these membranes and lipid drops, which had been recruited via PI4P, the virions can be assembled after viral-RNA replication. (wikipedia.org)
  • Moreover, insulin promotes lipid storage by inhibiting lipolysis. (isciii.es)
  • Therefore, these data demonstrate the essential role of lipid metabolism and LDs during + RNA viral replication. (biomedcentral.com)
  • EGCG had no effect on HCV RNA replication, assembly or release of progeny virions. (activeboard.com)
  • The N-terminus binds to cellular membranes and is essential for the formation of replication complexes. (wikipedia.org)
  • ADAR can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). (thermofisher.com)
  • Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). (thermofisher.com)
  • A critical role of a cellular membrane traffic protein in poliovirus RNA replication. (microbiologyresearch.org)
  • Curcumin has also been found to inhibit cell-to-cell transmission and to be effective in combination with other antiviral agents. (princeton.edu)
  • Interferon-α, which was previously the most common treatment for HCV, is a proinflammatory cytokine and can provoke a relapse of ulcerative colitis [ 10 ]. (biomedcentral.com)
  • Effects of interferon-a/ß on HBV replication determined by viral load PLoS Pathog. (usc.edu)
  • Sovaldi has shown a very high cure rate (nearly 100% HCV suppression and sustained virological response) in clinical trials of previously untreated patients and has fewer side effects than pegylated-interferon and ribavirin therapies. (michaelchimenti.com)
  • Helicases are enzymes that manipulate DNA and RNA in all aspects of nucleic acid metabolism. (uams.edu)
  • Enzymes in a metabolic pathway may be inhibited by molecules produced later in the pathway. (cp-673451inhibitor.com)
  • Bennasser Y, Le SY, Benkirane M, Jeang KT (2005) Evidence that HIV-1 encodes an siRNA and a suppressor of RNA silencing. (virosin.org)
  • Common themes to emerge from studies of picornavirus replication are the need for cholesterol and phosphatidylinositol 4-phosphate (PI4P). (microbiologyresearch.org)
  • Expression levels of all known HCV (co-)receptors were unaltered by EGCG. (activeboard.com)
  • Theoretically, nucleic acids-based antiviral therapeutics are dedicated to inhibiting the expression of viral or host factor genes via DNA or RNA. (creative-biolabs.com)
  • The result is a new generation of highly effective HCV therapeutics with few side effects that can make a significant difference in the lives of patients living with HCV. (michaelchimenti.com)
  • To this end, an increased dose of SOF was found to effectively decrease intracellular SARS-CoV-2 RNA levels. (mybestmedicine.com)
  • We also found that RNAi-mediated knockdown of the key Fe modulator hepcidin increased intracellular ferritin and inhibited HCV replication. (microbiologyresearch.org)
  • We propose that in Huh7 cells hepcidin modulation leads to an unfavourable intracellular environment for HCV replication. (microbiologyresearch.org)
  • A high degree of sequence and structural similarity was found between the RdRp of SARS-CoV-2 and HCV. (mybestmedicine.com)
  • A twist in the tail: SHAPE mapping of long-range interactions and structural rearrangements of RNA elements involved in HCV replication. (ox.ac.uk)
  • In this study, the structural requirement of sterols for infectivity, buoyant density and apolipoprotein association of HCV was investigated further. (microbiologyresearch.org)
  • These results point to a unique requirement towards lipids at the FMDV replication membranes. (microbiologyresearch.org)
  • Many studies have highlighted the close relationship between HCV life cycle and lipids metabolism (Herker and Ott, 2011). (cp-673451inhibitor.com)
  • Currently, nucleoside/nucleotide analogue (NA) therapy is the recommended first-line therapy for patients with chronic HBV because it can inhibit viral replication. (medscape.com)
  • Enhances the replication of MV, VSV and HIV-1 through an editing-independent mechanism via suppression of EIF2AK2/PKR activation and function. (thermofisher.com)
  • HCV induces the expression of Rubicon and UVRAG to temporally regulate the maturation of autophagosomes and viral replication PLoS Pathog. (usc.edu)
  • Compound HXL-10, a novel fused bicyclic derivative of pyrrolidine and imidazolidinone, was identified as a potent anti-HCV agent with a low cytotoxicity and high specificity. (bvsalud.org)
  • Autophagy inhibits oxidative stress and tumor suppressors to exert its dual effect on hepatocarcinogenesis Cell Death Differ. (usc.edu)
  • Picornaviruses form replication complexes in association with membranes in structures called replication organelles. (microbiologyresearch.org)
  • For the enteroviruses, replication organelles form at Golgi membranes in a process that utilizes PI4KIIIβ. (microbiologyresearch.org)
  • With the capability of cleaving RNA molecules with high efficiency and specificity, deoxyribozymes have been considered promising antiviral agents. (creative-biolabs.com)
  • Several other antisense RNAs as well as the more malleable small interfering RNA molecules ('-sirans') are currently in early-phase clinical trials. (medscape.com)