• In addition, INK4 proteins play roles in cellular senescence, apoptosis and DNA repair. (wikipedia.org)
  • The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
  • Tan IIA inhibited proliferation of mouse keratinocytes in a dose- and time-dependent manner and induced apoptosis, resulting in S phase arrest accompanied by down-regulation of pCdk2 and cyclin A protein expression. (hindawi.com)
  • Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
  • Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
  • Although the conventional activities of p53 such as cell cycle arrest, senescence, and apoptosis are well accepted as the major checkpoints in stress responses, accumulating evidence implicates the importance of other tumor suppression mechanisms. (nature.com)
  • Is p53-dependent ferroptosis sufficient for tumor suppression in the absence of cell cycle arrest, senescence, and apoptosis? (nature.com)
  • To date, various mechanisms have been suggested to explain the powerful tumor-suppressive effect of p53, including the induction of cell cycle arrest, senescence, and apoptosis. (nature.com)
  • Cell apoptosis and senescence-like morphology were also observed. (stanford.edu)
  • Akt pathways have effects on apoptosis, protein synthesis, metabolism and cell cycle. (springer.com)
  • Background: Apoptosis regulates normal development, homeostasis, immune tolerance and response to environmental stress by eliminating unwanted or diseased cells, and plays a key role in non-specific immunity of invertebrates. (bvsalud.org)
  • The exogenous pathway mediated by death receptors and death ligands is a very important pathway for cell apoptosis. (bvsalud.org)
  • The deep involvement of FasL in vertebrates cell apoptosis and immunity has been reported many times, but there is limited research on the FasL gene in shellfish, and its functional importance in oyster cell apoptosis and immunity remains unclear. (bvsalud.org)
  • induce apoptosis (cell death) of rapidly proliferating parasites and cancer cells! (cures.news)
  • Endogenous interleukin-4 promotes tumor development by increasing tumor cell resistance to apoptosis. (shengsci.com)
  • They can trigger apoptosis, a process of programmed cell death, when a cell becomes irreparably damaged or poses a risk of becoming cancerous. (pharmiweb.com)
  • Indeed apoptosis is inhibited in senescent cells. (globale-dermatologie.com)
  • The effects of CMTM4 on cellular apoptosis and cell cycle progression were analysed by flow cytometry, and cell cycle hallmarks were detected by western blotting and RT-PCR. (biomedcentral.com)
  • p53 can activate a variety of signaling pathways to inhibit tumors, such as cell cycle arrest, cell senescence, apoptosis or iron death. (medicaltrend.org)
  • Momordicine I also induced apoptosis, intracellular reactive oxygen species (ROS) production, and senescence in glioma cells. (excli.de)
  • 1632 remedy, even at a higher dose, did not drastically enhance apoptosis or senescence (Figures S8 and S9), suggesting that the inhibitory effects of C1632 on colony formation are not because of cytotoxicity-induced cell death. (emlinhibitor.com)
  • In other situations the response is cell cycle arrest or programmed cell death (apoptosis), providing a barrier to further tumour development that the tumour may ultimately circumvent through the acquisition of a mutation in one of the genes within the stress-response pathway. (southampton.ac.uk)
  • The p53 protein is an important tumor suppressor that is essential for regulating cell division, senescence, and self-destruction (apoptosis). (medlineplus.gov)
  • WM 1119 induces cell-cycle exit and cellular senescence, and inhibits growth of B-cell lymphoma cells in vitro (IC 50 = 0.25 μM). (rndsystems.com)
  • Several hallmarks of cellular senescence, such as cell cycle arrest, expression of cyclin-dependent kinase inhibitors, DNA damages, and senescence-associated secretory profile were evaluated. (aging-us.com)
  • Etoposide-induced senescence model may help investigate the initiation of cellular senescence in chondrocytes, and provide a useful model to develop therapeutic approaches to target senescence in OA. (aging-us.com)
  • Cellular senescence has been described as one of the major drivers of aging [ 6 ] and has been implicated in the pathogenesis of many age-related diseases [ 7 ]. (aging-us.com)
  • Recently, cellular senescence has emerged as a new target to treat OA [ 8 ]. (aging-us.com)
  • Because a specific marker for cellular senescence has yet to be identified, a combination of markers should be used to identify SnCs [ 14 ]. (aging-us.com)
  • Considering the role of cellular senescence in age-related diseases including OA, the therapeutic potential of senolytic (drugs that induced SnCs death) and senomorphic (drugs that modulate the SASP) compounds have been contemplated with growing interest [ 15 ]. (aging-us.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Keywords: SARS-CoV-2, cellular senescence, angiotensin II, prognosis, crucial illness, immune checkpoint inhibitors Introduction High transmissibility, asymptomatic service providers, and the absence of herd immunity have contributed to the quick worldwide spread of COVID-19 disease (1, 2). (ubatubasat.com)
  • We currently tested whether Berberine can affect premature, stress-induced cellular senescence caused by mitoxantrone. (aging-us.com)
  • In view of the evidence that BRB localizes in mitochondria, inhibits respiratory electron chain and activates AMPK, the observed attenuation of the replication stress-induced cellular senescence most likely is mediated by AMPK that leads to inhibition of mTOR signaling. (aging-us.com)
  • Previously, we reported that PAX3-FOXO1 supports aRMS initiation by enabling bypass of cellular senescence. (refine.bio)
  • We will dig into a few technical subjects such as NAD+, Autophagy, Mitophagy, and Cellular Senescence, Microbiome Swarming and Stem Cell Survival to understand why this protocol can be maximized to achieve a much stronger healing effect. (drjohnlieurance.com)
  • These little structures sit inside each of your cells, where they convert glucose and oxygen to create all the energy needed to run every aspect of all your cellular functions. (drjohnlieurance.com)
  • Tumor suppressor genes can promote cellular senescence, a state in which cells stop dividing, preventing the replication of damaged DNA. (pharmiweb.com)
  • The hsa-miR-371-373 cluster (counteracting cellular senescence and linked with differentiation potency), as well as hsa-miR-520c/-520h (inhibiting the tumor suppressor p21) were 3.9-16.3 fold up-regulated in two of the three cisplatin resistant cell lines. (biomedcentral.com)
  • Over recent decades, the field of cellular senescence has attracted considerable attention due to its association with aging, the development of age-related diseases and cancer. (liverpool.ac.uk)
  • Cellular dormancy and heterogeneity in cell cycle length provide important explanations for treatment failure after adjuvant therapy with S-phase cytotoxics in colorectal cancer (CRC), yet the molecular control of the dormant versus cycling state remains unknown. (ox.ac.uk)
  • Cellular senescence is a state of cell cycle arrest induced by repetitive cell mitoses or different stresses, which is implicated in various physiological or pathological processes. (hal.science)
  • We will also discuss the differential impacts of transient versus persistent status of cellular senescence, as well as the therapeutic potential of senescent cell targeting in these diseases. (hal.science)
  • Examples include exposure to toxic compounds or radiation, loss of contact with other cells or the extra-cellular matrix, lack of oxygen (hypoxia), acidic pH, the activation of oncogenes, induction of cellular senescence, oxidative damage or depletion of essential metabolites. (southampton.ac.uk)
  • CtBP activity is modified by UV radiation and glycolytic metabolism, suggesting that CtBPs regulate cell survival in response to cellular stress. (southampton.ac.uk)
  • PI3K-PKC pathway is a key monitor of mammalian cells regulated by its different isoenzymes, which may exert similar or opposite cellular effects by differential coupling of signaling pathways. (pi3k-inhibitors.com)
  • Cellular senescence was first identified as a type of irreversible cell cycle arrest that occurs when cells reach the end of their replicative potential ( Hayflick and Moorhead, 1961 ). (rupress.org)
  • Senescent cells also undergo dramatic changes in their morphology and in the organization and architecture of their cellular compartments. (rupress.org)
  • Senescent cells (SnCs) have been described to accumulate in osteoarthritis (OA) joint tissues in response to injury, thereby participating in OA development and progression. (aging-us.com)
  • Indeed, senescent cells (SnCs) are described to accumulate in joint tissues in response to injury and during aging, thereby participating in its development and progression [ 8 - 10 ]. (aging-us.com)
  • As viral replication is usually more efficient in senescent cells, many viruses, including CMV and probably SARS-CoV-2, promote this phenotype in host cells to facilitate invasion (19, 21, 22). (ubatubasat.com)
  • Inflammation plays a role in the progression to cancer and it is linked to the presence of senescent cells. (shengsci.com)
  • Boosting your mitochondria, and improving the clearing and recycling of these senescent cells are among the most effective strategies that I have seen clinically for combating aging and improving your general health. (drjohnlieurance.com)
  • Two schools of thought exist, one states that SK develops from an accumulation of senescent cells, whereas the other states that cells hyperproliferate. (globale-dermatologie.com)
  • Senescent cells are unable to proliferate, as the pathways responsible for initiating the cell cycle are irreversibly inhibited. (liverpool.ac.uk)
  • Nevertheless, senescent cells accumulate in tissues and develop a pro-inflammatory secretome, known as the senescence-associated secretory phenotype (SASP), which can have serious deleterious effects if not properly regulated. (liverpool.ac.uk)
  • There is increasing evidence suggesting senescent cells contribute to different stages of carcinogenesis in different anatomical sites, mainly due to the paracrine effects of the SASP. (liverpool.ac.uk)
  • We will also discuss different approaches to target senescent cells that might be of use for cancer treatment. (liverpool.ac.uk)
  • The beneficial or adverse effects of senescent cells depend on their transitory or persistent state. (hal.science)
  • On the other hand, persistent accumulation of senescent cells has been associated with chronic diseases and age-related illnesses like renal/urinary tract disorders. (hal.science)
  • The deleterious effects of persistent senescent cells have been related, in part, to their senescence-associated secretory phenotype (SASP) characterized by the release of a variety of factors responsible for chronic inflammation, extracellular matrix adverse remodeling, and fibrosis. (hal.science)
  • However, the accumulation of senescent cells can have detrimental consequences, such as in age-related pathologies. (rupress.org)
  • SnCs exhibit irreversible growth arrest accompanied by increased expression of cyclin-dependent kinase inhibitors (CDKi) such as p16 INK4a , and p21 Cip1 , accumulation of DNA damages, and secretion of diverse bioactive molecules known as the senescence-associated secretory phenotype (SASP). (aging-us.com)
  • The senescence-associated secretory phenotype (SASP) is a hallmark of senescence with an important physiological impact, but how it is established is unclear. (rupress.org)
  • However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)
  • Moreover, we describe for the first time an association of the up-regulation of micro-RNA species such as hsa-miR-512-3p/-515/-517/-518/-525 and down-regulation of hsa-miR-99a/-100/-145 with a cisplatin resistant phenotype in human germ cell tumors. (biomedcentral.com)
  • Inhibitors of histone acetyltransferases KAT6A/B induce senescence and arrest tumour growth. (rndsystems.com)
  • Chronic exposure to IL-1β induces only partial expression of senescence markers and does not allow us to conclude on its ability to induce senescence in chondrocytes. (aging-us.com)
  • The cell wall plays an important structural role for bacteria and is intimately tied to a variety of critical processes ranging from growth and differentiation to pathogenesis. (bvsalud.org)
  • Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)
  • On the other hand, etoposide treatment reliably induces DNA damage-related senescence in human articular chondrocytes evidenced by loss of proliferative capacity, DNA damage accumulation, and expression of some SASP components. (aging-us.com)
  • Their function, inhibiting CDK4/6, is to block progression of the cell cycle beyond the G1 restriction point. (wikipedia.org)
  • This miRNA functions as a tumor suppressor and dysregulation or loss of the host gene from which this miRNA is processed is associated with cancer progression in numerous cell types. (cancerindex.org)
  • It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. (thermofisher.com)
  • DTL (Cdc10-dependent transcript 2) is a critical regulator of cell cycle progression and genomic stability. (transhumanist.ru)
  • We hypothesized that a preneoplastic field of inflammation, telomere shortening, and senescence underlies tumor progression in UC progressors. (shengsci.com)
  • During cancer progression, some tumor cells acquire new characteristics, such as over-expression of epithelial-mesenchymal transition (EMT) markers, and undergo profound morphogenetic changes. (biomedcentral.com)
  • Genes necessary for cell cycle progression, such as E2F-dependent genes, are incorporated into the SAHF and are thereby silenced, contributing to the stability of the growth arrest. (rupress.org)
  • However, binding of p16(INK4A) blocks CDK4's or CDK6's ability to stimulate cell cycle progression. (medlineplus.gov)
  • Targeting DTL induces cell cycle arrest and senescence and suppresses cell growth and colony formation through TPX2 inhibition in human hepatocellular carcinoma cells. (transhumanist.ru)
  • RASSF4 loss-of-function activates Hippo/Mst1 and inhibits downstream YAP, causing aRMS cell cycle arrest and senescence. (refine.bio)
  • Once activated, p53 coordinates multiple downstream pathways, thereby maintaining the homeostasis of the host cell or organism (if the stress is mild, transient, and repairable) or eliminating damaged cells (if the stress is acute, prolonged, and difficult to resist). (nature.com)
  • The mechanisms of radioresistance are still poorly understood, despite it has been suggested that miRNAs play an important role in cell signaling pathways. (frontiersin.org)
  • Indeed, it has been shown that miRNAs play an important role in gene expression, mainly when associated with the monitoring of several cell and metabolic pathways, being also an essential component of the gene silencing machinery in most eukaryotic organisms ( 4 , 8 ). (frontiersin.org)
  • NF-κB pathways are associated with immunity, inflammation and cell survival. (springer.com)
  • AMPK pathways inhibit biosynthetic pathways with energy consumption, such as protein, fatty acid and glycogen synthesis. (springer.com)
  • However, it is known that many of the signaling pathways activated by Stxs regulate the expression of multiple genes in mammalian cells. (refine.bio)
  • Signaling pathways that are activated by intrinsic or extrinsic stimulation converge on the transcriptional factors and regulate phenotypic changes of cancer cells [ 9 ]. (biomedcentral.com)
  • In single celled organisms these pathways are generally involved in ensuring the survival and replication of the individual cell. (southampton.ac.uk)
  • Stress-response pathways play a key role in the patho-physiology and treatment of many diseases, including cancer.At almost every stage of the development of a tumour, cells are exposed to some form of stress. (southampton.ac.uk)
  • Many current and prospective treatments for cancer work by either inhibiting, or re-activating stress response pathways.Our work focuses on the role of regulators of gene transcription in the response of cancer cells to stress. (southampton.ac.uk)
  • A particular interest of our work has been how HDM2 and HDMX protein synthesis is controlled in response to cell-signalling pathways in different cell types, and how this affects p53 function in these cells. (southampton.ac.uk)
  • Targeting specific pathways which are aberrant in cancer cells relative to normal cells is ideally a good approach at therapeu- tic dosages. (pi3k-inhibitors.com)
  • The most promising candidate, CYT997, was tested for its invitro effects on cell viability and cell death, colony formation, cell cycle changes, and cell migration/invasion in HCC cells. (stanford.edu)
  • This pitfall may be due to the lack of understanding of the mechanisms underlying chondrocyte senescence. (aging-us.com)
  • Hence, it is of great importance to investigate the mechanisms that induce enzalutamide resistance in prostate cancer cells. (biomedcentral.com)
  • Thus, it is now possible to study cell wall biogenesis mechanisms within a member of the Tissierellia, which may also reveal novel aspects of P. micra pathobiology. (bvsalud.org)
  • Mechanisms of pathogenesis of atherosclerosis are complex interplay between bloodstream cells and arterial wall components that leads to a chronic state of vascular oxidative stress and inflammation ( Hansson and Hermansson, 2011 ). (frontiersin.org)
  • Furthermore, our results provide insights into molecular mechanisms for targeting DTL in liver cancer cells. (transhumanist.ru)
  • To expand the scope of analysis of gene expression and to better understand the underlying mechanisms for the various effects of Stxs on cell functions, we carried out comparative microarray analyses to characterize the global transcriptional response of human macrophage-like THP-1 cells to Shiga toxin type 1 (Stx1) and LPS. (refine.bio)
  • We compared microRNA expression patterns in three cisplatin resistant sublines derived from paternal cisplatin sensitive germ cell tumor cell lines in order to improve our understanding of the mechanisms of cisplatin resistance. (biomedcentral.com)
  • In this review, we aim to discuss the molecular mechanisms underlying the senescence response and its relationship with cancer development, focusing on the link between senescence-related inflammation and cancer. (liverpool.ac.uk)
  • In this review, we will summarize the molecular mechanisms of senescence and their implication in renal and urinary tract diseases. (hal.science)
  • Evidence has been provided that activation of the vascular endothelial cells in the presence of the risk factors promotes oxidative stress and vascular inflammatory responses, leading to acceleration of atherosclerotic vascular disease. (frontiersin.org)
  • Moreover, momordicine I decreased the oxidative phosphorylation capacity of glioma cells and inhibited tumor sphere formation in temozolomide (TMZ)-resistant GBM cells. (excli.de)
  • Our data reveal consistent ENKUR downregulation at both RNA and protein levels across MPN patient specimens and experimental models, with a concomitant upregulation of a cell cycle marker, CDC20. (stanford.edu)
  • DTL depletion resulted in the disruption of the mitotic proteins cyclin B, CDK1 , securin, seprase, Aurora A, and Aurora B as well as the upregulation of the cell cycle arrest gene [i]p21[/i]. (transhumanist.ru)
  • As a result, angiotensin II (ANG II) accumulates in endothelial cells (ECs), inducing vascular senescence with upregulation of interleukin-6 (IL-6) and reactive oxygen species (ROS), impairing both innate and adaptive immunity. (ubatubasat.com)
  • Indeed, as in avian influenza, the Pitavastatin Lactone upregulation of NK cell, and CTC exhaustion markers (EMs) has been observed (11). (ubatubasat.com)
  • Indeed, SARS-CoV-2 has been associated with upregulation of ANG II, a molecule previously Pitavastatin Lactone shown to promote senescence in vascular easy muscle mass cells (VSMCs) and endothelial cells (ECs) (24C26). (ubatubasat.com)
  • We hypothesize that vascular senescence-mediated upregulation of IL-6 and ROS is responsible for both coagulation and immune dysfunction. (ubatubasat.com)
  • Interestingly, treatment with low concentrations of Salinomycin induced a transient G1 arrest at earlier time point and G2 arrest at later point and senescence associatedwith enlarged cellmorphology, upregulation of p21 protein, increase in histone H3 and H4 hyperacetylation and expression of SA-β-Gal activity. (uaeu.ac.ae)
  • Restoration of CMTM4 significantly suppressed 786-O cell growth by inducing G2/M cell cycle arrest and p21 upregulation, and cell migration was also inhibited. (biomedcentral.com)
  • Zhao H , Halicka HD , Li J , Darzynkiewicz Z , . Berberine suppresses gero-conversion from cell cycle arrest to senescence. (aging-us.com)
  • We also show that ARF promotes p53 mutant stability in tumors and suppresses p73 mediated p21 expression and senescence. (oncotarget.com)
  • Primary clear cell renal cell carcinoma (ccRCC) and the paired adjacent non-tumour tissues were then collected to examine the expression of CMTM4 by western blotting, immunohistochemistry, and quantitative RT-PCR. (biomedcentral.com)
  • Western blotting then confirmed that CMTM4 was dramatically reduced in 86.9 % (53/61) of ccRCC tissues compared with the paired adjacent non-tumour tissues, as well as in the 786-O and A498 ccRCC cell lines. (biomedcentral.com)
  • Overexpression of CMTM4 also markedly inhibited the tumour xenograft growth in nude mice. (biomedcentral.com)
  • p values have been calculated working with the unpaired Student's t test ( p 0.001)addition, C1632 suppressed the growth of xenograft tumour cells in a time- dependent manner (Figure 7C). (emlinhibitor.com)
  • In some circumstances, the activation of a stress-response pathway will actually help the tumour cell to survive and proliferate. (southampton.ac.uk)
  • Effects of tumor cell viability and inoculum density on growth parameters in the human tumor, soft-agar clonogenic assay. (shengsci.com)
  • Methods Cell viability was measured by CellTiter-Glo and Trypan blue exclusion assay. (uaeu.ac.ae)
  • We tested four major extracts of M. charantia and found that momordicine I reduced glioma cell viability without serious cytotoxic effects on astrocytes. (excli.de)
  • ST can induce both cell viability loss and cell senescence, and it can cause G1-S cell cycle WEHI-345 arrest and the DNA damage response in OS-RC-2 cells12,13. (euromed2016.com)
  • Invivo, CYT997 (20mg/kg, three times weekly by oral gavage) significantly inhibited PDX growth, while being non-toxic to mice. (stanford.edu)
  • Our results demonstrate that tumor growth, peritoneal dissemination and peritoneum or organ metastasis implanted MKN45 cells were significantly decreased in shAhR and Biseugenol-treated mice and that endoplasmic reticulum (ER) stress was caused. (oncotarget.com)
  • DTL depletion inhibited liver cancer cell growth, increased senescence, and reduced tumorigenesis. (transhumanist.ru)
  • A rescue assay indicated that DTL should be targeted through TPX2 downregulation for cancer cell growth inhibition. (transhumanist.ru)
  • Moreover, DTL silencing inhibited the growth of patient-derived primary cultured HCC cells. (transhumanist.ru)
  • Cycles of probiotic growth periods through natural circadian rhythm stimulus. (drjohnlieurance.com)
  • Cells are potent to go under abnormal growth processes and finally lead to tumor/cancer. (pharmiweb.com)
  • Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
  • In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
  • Results Salinomycinwas able to inhibit the growth of the three cell lines in time- and concentration-dependent manners. (uaeu.ac.ae)
  • Cell Counting Kit-8 (CCK-8) and viable cell counting assays were used to delineate the growth curves of 786-O cells after CMTM4 overexpression or knockdown. (biomedcentral.com)
  • Human CRC cell dormancy and tumor growth can also be perturbed by itraconazole, which is found to inhibit Wnt signaling through noncanonical hedgehog signaling. (ox.ac.uk)
  • It's no secret to readers here that DrJohn and DrGeorge have day jobs as stem cell researchers, both in the lab and the clinic, and have developed skincare products whose hero ingredients (human growth factors, cytokines, mRNAs,miRNAs, etc) derive from stem cells on permanent loan from bone marrow of young adult humans. (barefacedtruth.com)
  • Whether within the body or in a laboratory flask, growth factors and cytokines patterns are determined by the genetic material within the cells that produce them. (barefacedtruth.com)
  • These are the three most commonly cultured cells used to produce growth factors and cytokines for skincare products. (barefacedtruth.com)
  • Somatic mutations in other genes involved in cell growth are also needed for a melanoma to develop. (medlineplus.gov)
  • Together, the germline and somatic mutations impair the function of proteins that regulate division and senescence, leading to uncontrolled cell growth and the formation of a melanoma. (medlineplus.gov)
  • CDKN2A gene mutations involved in cancer impair production of functional p16(INK4A) or, less commonly, p14(ARF), which can result in uncontrolled cell growth and tumor formation. (medlineplus.gov)
  • INK4 proteins are cell-cycle inhibitors. (wikipedia.org)
  • Binding of KLRG1 to E-, N-, or R- cadherins blocks phosphorylation of Akt and increases the expression of cell cycle inhibitors. (biolegend.com)
  • Therefore, we hypothesized the improved anticancer efficacy of QUE in combination with isoenzyme inhibitors-rottlerin (ROT-PKCδ inhibitor), G0 6983 (PKCα inhibitor), and PI-103 (p110α-class I PI3K inhibitor) in MCF-7 and RAW 264.7 cells. (pi3k-inhibitors.com)
  • The defining characteristic of senescence is a highly stable cell cycle arrest, triggered by the up-regulation of cyclin-dependent kinase inhibitors such as p16 INK4a and p21 CIP1a . (rupress.org)
  • Our study results indicate that DTL is a potential novel target gene for treating liver cancer through liver cancer cell senescence induction. (transhumanist.ru)
  • Furthermore, the induction of senescence was associated with enhancing reactive oxygen species (ROS) level. (scite.ai)
  • p53's ability to inhibit tumors can be considered as a specific outcome of its stress-responsive effects. (nature.com)
  • Different levels of viable cell inoculum density produced contrasting effects between 255 solid tumors as compared to 72 malignant. (shengsci.com)
  • Tumor occurrence is often a slow process, so even if the content of p53 does not increase, tumors can be inhibited by increasing the activity. (medicaltrend.org)
  • Our approach was designed to characterize the role of micro-RNAs on the presumably multifactorial phenomenon of acquired cisplatin resistance in germ cell tumors. (biomedcentral.com)
  • Wound healing and transwell assays were performed to assess the cells' ability to migrate. (biomedcentral.com)
  • The expression position of ChFasL in HEK293T cells was also located by subcellular localization, and the effect of increased recombinant protein content on the activity of reporter genes p53 and p21 was studied by dual-fluorescence reporter gene. (bvsalud.org)
  • In addition, high levels of infiltrating leukocytes were associated with telomere shortening, senescence, and reduced p53 expression. (shengsci.com)
  • To date, approaches to studying the capacity of Stxs to alter gene expression in intoxicated cells have been limited to individual genes. (refine.bio)
  • Let us underline nevertheless that such cytokeratin expression is found in embryonic cell lines and that the study only concerned 10 specimens. (globale-dermatologie.com)
  • The ccRCC cell lines A498 and 786-O and the normal renal tubular epithelial cell line HK-2 were also tested for CMTM4 expression by western blotting. (biomedcentral.com)
  • The traditional theory is that when cells are stimulated, the binding of MDM2 and p53 will be destroyed, leading to the accumulation of p53 protein, which affects downstream gene expression and enables cells to respond to the stimulus. (medicaltrend.org)
  • We further explored whether the antiglioma effect of momordicine I may be related to cell cycle modulation and DLGPA5 expression. (excli.de)
  • CYT997 inhibited HCC cell migration and invasion, and down-regulated the expressions of acetylated tubulins, beta-tubulin, glypican-3 (GPC3), beta-catenin, and c-Myc. (stanford.edu)
  • F I G U R E 3 C1632 inhibits the migration and invasion of NSCLC A549 and A549R cells. (emlinhibitor.com)
  • D) Quantification of the results in (C). (E) C1632 inhibits migration and invasion of A549R cells in the Transwell assay. (emlinhibitor.com)
  • Surprisingly, inhibition of AurA by AKI603 induced leukemia cell senescence in both BCR-ABL wild type and T315I mutation cells. (scite.ai)
  • Preclinical validation shows itraconazole to be effective in multiple assays through Wnt inhibition, causing both cycling and dormant cells to switch to global senescence. (ox.ac.uk)
  • Some tumor suppressor proteins are involved in maintaining cell adhesion, preventing cells from breaking away and spreading to other parts of the body (metastasis). (pharmiweb.com)
  • Recently obtained evidence reveals that epithelial-mesenchymal transition (EMT) of tumor cells causes metastasis, which is detrimental to patients' survival. (biomedcentral.com)
  • Only the has-miR-10b involved in breast cancer invasion and metastasis and has-miR-512-3p appeared to be up-regulated (2-3-fold) in all three cell lines. (biomedcentral.com)
  • This study used etoposide, to induce DNA damage-related senescence or chronic exposure to IL-1β to entail inflammation-related senescence in human OA chondrocytes. (aging-us.com)
  • However, chronic inflammation can also elicit this response by prolonged activation of T cell receptors (TCRs) (14). (ubatubasat.com)
  • Ulcerative colitis-associated colorectal cancer arises in a field of short telomeres, senescence, and inflammation. (shengsci.com)
  • Multiple biopsies of varying histologic grade were collected along the colon of nine UC progressors and analyzed for telomere length, DNA damage, senescence, p53, p16, and chronic and acute inflammation. (shengsci.com)
  • These results suggest that dysplasia arises in a preneoplastic field of chronic inflammation, which leads to telomere shortening, DNA damage, and senescence. (shengsci.com)
  • They facilitate adhesion between leukocytes and endothelial cells, enabling leukocyte extravasation into tissues during inflammation. (pharmiweb.com)
  • VCAMs are involved in mediating the adhesion of leukocytes to vascular endothelial cells, facilitating the recruitment of immune cells during inflammation. (pharmiweb.com)
  • PP2Abeta Additionally, ST has been demonstrated to inhibit AMPK and cause myocyte cytotoxicity16,17. (euromed2016.com)
  • Through enhancing Thr172 phosphorylation of the catalytic subunit and inhibiting dephosphorylation of Thr172, AMP binding was found to increase the activity of AMPK. (euromed2016.com)
  • Mammalian target of rapamycin complex 1 (mTORC1) is definitely a multiprotein complex consisting of mTOR, raptor, mLST8, and PRAS40, and AMPK demonstrated to inhibit mTORC1 via activating tuberous sclerosis complex 2 (TSC2) and directly reducing the phosphorylation of raptor18,19. (euromed2016.com)
  • Consequently, AMPK has been thought to result in autophagy through an indirect mechanism by inhibiting mTORC1 activity or directly binding to ULK1, which is a serine/threonine kinase and also known as ATG120,21. (euromed2016.com)
  • Indeed, compound C, a pharmacological AMPK inhibitor that efficiently blocks the metabolic actions of AMPK, has been demonstrated to induce the autophagic process in different malignancy cell lines23. (euromed2016.com)
  • ST was demonstrated to inhibit AMPK activity, upregulated p62 manifestation and abolished the H2O2-induced autophagic flux. (euromed2016.com)
  • PECAM-1 is expressed on the surface of platelets and endothelial cells and is involved in cell adhesion and signaling during immune responses. (pharmiweb.com)
  • Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
  • Neither AT alone in control media nor conditioned media nor AT-stimulated cells affected numbers of endothelial cells in the plug or plug's vascularization. (hindawi.com)
  • The process of neovascularization can be influenced not only by the residual endothelial cells but also by the circulating cells. (hindawi.com)
  • By using a direct in vivo shRNA screen, we show that liver cancer cells that have mutations in the gene encoding the tumor suppressor protein p53 (Trp53 in mice and TP53 in humans) and that are driven by the oncoprotein NRAS become addicted to MYC stabilization via a mechanism mediated by aurora kinase A (AURKA). (scite.ai)
  • Our results demonstrated that targeting DTL reduced cell cycle regulators and chromosome segregation genes, resulting in increased cell micronucleation. (transhumanist.ru)
  • Background In the present study, we investigated the effect of Salinomycin on the survival of three human breast cancer cell lines MCF-7, T47D and MDA-MB-231 grown in adherent culture conditions. (uaeu.ac.ae)
  • Therefore, the authors concluded to a probable hyperplasic status in benign or malignant neoplasic cells. (globale-dermatologie.com)
  • Transient senescence has major beneficial roles promoting successful post-injury repair and inhibiting malignant transformation. (hal.science)
  • Global transcriptional response of macrophage-like THP-1 cells to Shiga toxin type 1. (refine.bio)
  • MDM2 can either ubiquitinate p53 to promote its degradation, or directly bind p53 to inhibit its transcriptional activation function. (medicaltrend.org)
  • According to this model, it is speculated that if p53 can not be inhibited by various negative transcriptional regulatory proteins, even if its level is not significantly increased, it may be able to exert its normal tumor suppressor function. (medicaltrend.org)
  • For example, a Nature article in Gu Wei's laboratory in 2016 found that the SET protein can selectively bind to the unacetylated C-terminal domain of p53 and inhibit the transcriptional activation function of p53. (medicaltrend.org)
  • We used an HCC patient-derived xenograft (PDX) mouse model to assess its invivo efficacy.RESULTS: CYT997 was significantly more cytotoxic against HCC cells than against primary human hepatocytes, and sensitized HCC cells to sorafenib. (stanford.edu)
  • We created an in vitro model of acquired cisplatin resistance by long term exposure of three well established germ cell tumor cell lines to cisplatin, resulting in sublines with significantly increased resistance to cisplatin. (biomedcentral.com)
  • QUE significantly improves the cytotoxicity of ROT + G0 6983 ranged 30-55% and PI-103 ranged 24-63% after 24-48 h against MCF-7 cells. (pi3k-inhibitors.com)
  • Among the superfamily of cell adhesion molecules, in this article, we mainly focus on Ig-Superfamily CADMs: the members of the Immunoglobulin superfamily of cell adhesion molecules, which play diverse roles in immune responses, neural development, and cell signaling. (pharmiweb.com)
  • Conclusion Our data are the first to link senescence and histone modifications to Salinomycin. (uaeu.ac.ae)
  • Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)
  • In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)
  • To intelligently employ bio-signaling molecules in one's medical practice (or one's home skincare regimen), there should be familiarity with the significant differences among the bio-signals patterns produced by adipose (fat) stem cells and bone marrow mesenchymal stem cells, and for completeness, fibroblasts. (barefacedtruth.com)
  • Two weeks right after injection using the cancer cell inoculum, and after that every single two days thereafter, mice have been injected within the caudal vein with 30 mg/kg C1632. (emlinhibitor.com)
  • Treatment did not impact the physique weight of mice Adenosine A1 receptor (A1R) Purity & Documentation inoculated with A549R cells (Figure 7D). (emlinhibitor.com)
  • 2013). Same combination in polymeric micelles reduced the doxorubicin-induced cardiotoxicity in SKOV-3 and H9C2 cells as well as in mice, suggesting to limit the adverse effects of chemotherapy (Cote et al. (pi3k-inhibitors.com)
  • Senescence-associated β-Gal assay was used to detect the cell senescence level. (biomedcentral.com)
  • γ-H2AX staining assay was performed to assess the DNA damages of PCa cells. (biomedcentral.com)
  • Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
  • C) C1632 inhibits migration of A549R cells inside the scratch-wound healing assay. (emlinhibitor.com)
  • In cells, p53 is a very unstable protein with a short half-life. (medicaltrend.org)
  • DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)
  • In vitro and in vivo experiments showed that SNHG4 overexpression markedly enhanced cell resistance to enzalutamide. (biomedcentral.com)
  • In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)
  • We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
  • Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
  • The molecules are found on the cell surface, where they facilitate adhesion to neighboring cells or the extracellular matrix, creating stable cell structures and supporting tissue organization. (pharmiweb.com)
  • A and B) C1632 decreases cell adhesion to extracellular matrix. (emlinhibitor.com)
  • Indeed, in a clone of cells, only one of the alleles is expressed, because the same X chromosome is inactivated by lyonisation{Allen, 1992}, in a clone, whereas in normal {Nakamura, 2001} skin (polyclonal), the inactivated HUMARA gene allele isn't always on the same X chromosome. (globale-dermatologie.com)
  • Mutations in the CDKN2A gene are found in up to one-quarter of head and neck squamous cell carcinomas (HNSCC). (medlineplus.gov)
  • Mutations in the CDKN2A gene are also associated with melanoma, a type of skin cancer that begins in pigment-producing cells called melanocytes. (medlineplus.gov)
  • One of the treatments applied in cancer is radiotherapy (RT), a therapeutic modality that uses ionizing radiation to induce damage in unwanted cells. (frontiersin.org)
  • With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
  • Our results indicate that the cytotoxic effect of momordicine I on glioma cells suggests its potential therapeutic application to GBM treatment. (excli.de)
  • Unexpectedly, we demonstrate that dormant CRC cells are differentiated, yet retain clonogenic capacity. (ox.ac.uk)
  • Killer cell lectin-like receptor subfamily G member 1 (KLRG1) is a 30 kD, type II membrane glycoprotein with one C-type lectin domain and one immunoreceptor tyrosine-based inhibititory motif (ITIM). (biolegend.com)
  • The increase of interleukin-4 (IL-4) level in tumor environment and the up-regulation of IL-4 receptor (IL-4R) on tumor cells have been long observed. (shengsci.com)
  • Among these unconventional activities, an iron-dependent form of non-apoptotic cell death, termed ferroptosis, attracts great interest. (nature.com)
  • The initiation and development of tumor cell is accompanied by elevated stresses, such as oncogene activation, DNA damage, genome instability, and reprogrammed tumor metabolism. (nature.com)
  • Three cisplatin resistant sublines (NTERA-2-R, NCCIT-R, 2102EP-R) showing 2.7-11.3-fold increase in drug resistance after intermittent exposure to increasing doses of cisplatin were compared to their parental counterparts, three well established relatively cisplatin sensitive germ cell tumor cell lines (NTERA-2, NCCIT, 2102EP). (biomedcentral.com)
  • The paternal cell lines are (1) the p53-wt (wild type) pluripotent gonadal germ cell tumor cell line NTERA-2, (2) the p53-mutated pluripotent extragonadal nonseminomatous germ cell tumor cell line NCCIT and (3) the p53-wt nullipotent embryonal cell carcinoma 2102EP, derived from a primary human testicular teratocarcinoma. (biomedcentral.com)