Inhibits adipogenesis in 3t3 l1 cells. Medical search. Frequent questions

Adipogenesis can be described as the effective differentiation of preadipocytes into mature adipocytes that can then undergo lipogenesis. (wikipedia.org)
We show that piceatannol inhibits adipogenesis of 3T3-L1 preadipocytes in a dose-dependent manner at noncytotoxic concentrations. (oregonstate.edu)
In the early phase of adipogenesis, piceatannol-treated preadipocytes displayed a delayed cell cycle entry into G(2)/M phase at 24 h after initiation of adipogenesis. (oregonstate.edu)
Hirsutenone Directly Targets PI3K and ERK to Inhibit Adipogenesis in 3T3-L1 Preadipocytes. (oregonstate.edu)
Using Oil Red O staining, we observed that HST dose-dependently suppresses lipid accumulation during adipogenesis in 3T3-L1 preadipocytes, concomitant with a decrease in peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα) and fatty acid synthase (FAS) protein expression. (oregonstate.edu)
This inhibitory effect was largely limited to the early stage of adipogenesis, which includes mitotic clonal expansion (MCE), as evidenced by delayed cell cycle entry of preadipocytes from G1 to S phase. (oregonstate.edu)
Our results suggest that HST attenuates adipogenesis by directly targeting PI3K and ERK during MCE in 3T3-L1 preadipocytes, underscoring the potential therapeutic application of HST in preventing obesity. (oregonstate.edu)
We present the first evidence that a Wnt antagonist, Dickkopf 1 (Dkk1), is secreted by human preadipocytes and promotes adipogenesis. (ox.ac.uk)
In addition, constitutive expression of Dkk1 in 3T3-L1 preadipocytes promotes their differentiation, further supporting the functional significance of increased Dkk1 levels during human adipogenesis. (ox.ac.uk)
Notably, Dkk1 is not expressed in primary murine preadipocytes or cell lines. (ox.ac.uk)
Main methods: Cell viability of 3T3-L1 preadipocytes was assessed by MTT assays, and lipid accumulation was measured by Oil Red O. Adipogenesis related factors were checked by Western blot analysis. (kist.re.kr)
Adipogenesis has been described as a two-step developmental process consisting on the commitment of undifferentiated MSCs into preadipocytes and the further development of these cells into fully functional mature adipocytes [ 4 ]. (biomedcentral.com)
We have previously shown that fish primary preadipocytes differentiate into mature adipocytes in vitro and that these cells represent a very helpful model system to study adipose tissue development in fish [ 5 , 6 ]. (biomedcentral.com)
A metabolite of daidzein, 6,7,4'-trihydroxyisoflavone, suppresses adipogenesis in 3T3-L1 preadipocytes via ATP-competitive inhibition of PI3K. (oregonstate.edu)
Our objective was to determine the effects of 6,7,4'-THIF on adipogenesis in 3T3-L1 preadipocytes and elucidate the mechanisms of action involved. (oregonstate.edu)
Adipogenesis was stimulated in 3T3-L1 preadipocytes. (oregonstate.edu)
Our results suggest that 6,7,4'-THIF suppresses adipogenesis in 3T3-L1 preadipocytes by directly targeting PI3K. (oregonstate.edu)

3T3-L1 cells have a fibroblast-like morphology, but, under appropriate conditions, the cells differentiate into an adipocyte-like phenotype, providing an exemplar model for white adipocytes. (wikipedia.org)
In 1971, Green and Kehinde established a subline of 3T3 cells, called 3T3-L1, which demonstrated a greater propensity for differentiation into adipocytes and a higher affinity for lipid deposition. (wikipedia.org)
In the early 1980s, it was discovered that these cells could be induced to differentiate into adipocytes in response to hormonal stimulation. (wikipedia.org)
3T3-L1 cells are commonly utilized as an effective model for white adipocytes because of their similar fibroblast-like morphology, lipid accumulation, gene expression, and basal energy exchanges and transformations. (wikipedia.org)
Although, some literature suggests that 3T3-L1 adipocytes can possess certain phenotypic signatures similar to other adipocyte lineages aside from white adipocytes. (wikipedia.org)
Acer Tegmentosum Maxim Inhibits Adipogenesis in 3t3-l1 Adipocytes and " by Hang-Hee Cho, Soo-Jung Lee et al. (touro.edu)
Protein kinase B (Akt) and glycogen synthase kinase 3β (GSK3β) phosphorylation was also decreased by ATM treatment of 3T3-L1 adipocytes. (touro.edu)
Results: In adipose tissues, GREM2 expression was observed in cells, including ASCs, but not in mature adipocytes, and the expression level per cell increased with age. (fujita-hu.ac.jp)
The present study discusses the efficacy of Aloe emodin-8-O-glycoside (AEG), a plant derived anthroquinone, on alleviating insulin resistance and augmenting glycogen synthesis in L6 myotubes and 3T3L1 adipocytes. (wiley.com)
Thus, in both differentiating and differentiated 3T3-L1 adipocytes, high concentrations of melatonin increased intracytoplasmic triglyceride accumulation, an indicator of adipogenic differentiation. (unican.es)
These findings suggest that, in human breast tumors, melatonin could stimulate the differentiation of fibroblasts and reduce the aromatase activity and expression in both fibroblasts and adipocytes, thereby reducing the number of estrogen-producing cells proximal to malignant cells. (unican.es)
In order to understand the development of adiposity, it is crucial to identify the factors and mechanisms that regulate the recruitment of mesenchymal stem cells (MSCs) of the vascular stromal fraction of the adipose tissue and its transformation into lipid-filled adipocytes. (biomedcentral.com)

Piceatannol, natural polyphenolic stilbene, inhibits adipogenesis via modulation of mitotic clonal expansion and insulin receptor-dependent insulin signaling in early phase of differentiation. (oregonstate.edu)
Cell cycle studies indicated that PLH halts the mitotic clonal expansion. (bvsalud.org)
The study suggested that PLH inhibited adipogenesis during the early phase of differentiation by targeting mitotic clonal expansion and arresting the cell cycle in the G1 phase of the cell cycle. (bvsalud.org)
Flow cytometry was used to analyze the mitotic cell cycle during the mitotic clonal expansion phase. (kist.re.kr)
Significance: These findings suggest that apigenin can prevent 3T3-L1 preadipocyte differentiation by the inhibition of the mitotic clonal expansion and the adipogenesis related factors and upregulation of the expression of multiple C/EBPβ inhibitors. (kist.re.kr)

The 3T3-L1 cell line is a sub clone that was initially developed from a mouse embryo, from a clonal expansion of Swiss 3T3 cells. (wikipedia.org)
Apigenin-treated 3T3-L1 cells failed to undergo clonal expansion during the early phase of adipocyte differentiation. (kist.re.kr)

Cellular lipid content in DMI (dexamethasone, 3-isobutyl-1-methylxanthine, and insulin mixture)-treated cells increased, while ATM treatment caused a significant reduction in lipid accumulation in differentiated 3T3-L1 cells. (touro.edu)
Risperidone increases adipocyte lipid accumulation by plausibly inhibiting long-chain fatty acid β-oxidation through targeting MAPK14 and MAPK8. (frontiersin.org)
6,7,4'-THIF significantly suppressed MDI-induced lipid accumulation in the early stage of adipogenesis, attributable to a suppression of cell proliferation and the induction of cell cycle arrest. (oregonstate.edu)

3T3-L1 cells of the adipocyte morphology increase the synthesis and accumulation of triglycerides and acquire the signet ring appearance of adipose cells. (wikipedia.org)

We investigated the effect of Acer tegmentosum Maxim (ATM) on adipocyte differentiation in 3T3-L1 cells and anti-obesity properties in obese rats fed a high-fat diet (HFD). (touro.edu)

Cell proliferation was reduced in a time-dependent manner of drug exposure as revealed from BrdU incorporation and migration studies. (jcimjournal.com)
Effects of Thuja occidentalis ethanolic extract on cellular proliferation tested by BrdU incorporation assay Cells were exposed to three different concentration of Thuja occidentalis ethanolic extract for 6, 12 and 24 h along with incorporation of BrdU. (jcimjournal.com)
The possible mechanism was related to induction of apoptosis, inhibition of tumor HeLa cell proliferation by decreasing the Ki-67 protein expression, and prevention of tumor angiogenesis by reducing VEGF and CD31 protein expression in HeLa cells. (bvsalud.org)
SCARA5 overexpression significantly inhibited cell proliferation and induced apoptosis of CAL-27 and SCC-9 cells. (degruyter.com)
In conclusion, SCARA5 inhibited the proliferation and EMT and induced the apoptosis of OSCC cells through the inhibition of STAT3 and PI3K/AKT signaling pathways, thereby exerting a tumor suppressor effect. (degruyter.com)
Overall, our study demonstrates the coordinated expression of functionally related genes during proliferation and differentiation of rainbow trout adipocyte cells. (biomedcentral.com)

ATM (60 ug/mL) caused inhibition of adipogenesis via down-regulation of the CCAAT/enhancer binding protein β (C/EBPβ) (48%), C/EBPα (66%), and peroxisome proliferator-activated receptor γ (PPARγ) (64%) expressions in 3T3-L1 cells. (touro.edu)

6,7,4'-THIF was found to inhibit PI3K activity via direct binding in an ATP-competitive manner. (oregonstate.edu)

Concomitant downregulation of the Dkk1 receptors LRP5 and LRP6 is likely to potentiate the ability of Dkk1 to inhibit Wnt signalling and promote differentiation. (ox.ac.uk)

3T3-L1 cells can be utilized to study a number of cellular and molecular mechanisms related to insulin-resistance, obesity, and diabetes in vitro. (wikipedia.org)
Adipogenesis is a key driver of the expansion of adipose tissue mass that causes obesity. (oregonstate.edu)
Notoginsenosides can treat obesity by reducing lipid synthesis, inhibiting adipogenesis, promoting white adipose tissue browning, increasing energy consumption, and improving insulin sensitivity. (frontiersin.org)
Additionally, pentagalloylglucose and tannin acids inhibit adipogenesis and counteract obesity [ 8 - 10 ]. (hindawi.com)
in addition, obesity increases the production of IL-1β, which may have cytotoxic effects on beta islet cells [7]. (heraldopenaccess.us)

Piceatannol dose-dependently inhibited differentiation mixture-induced phosphorylation of insulin receptor (IR)/insulin receptor substrate-1 (IRS-1)/Akt pathway in the early phase of adipogenesis. (oregonstate.edu)

To study the possible anticancer and antiproliferative activities of ethanolic leaf extract of Thuja occidentalis (TO) on A549 non-small lung carcinoma cells in vitro . (jcimjournal.com)
Ethanolic leaf extract of TO demonstrated apoptotic and antiproliferative potentials against A549 cell line. (jcimjournal.com)
Effects of Thuja occidentalis ethanolic extract on viability of A549 cell line (A) and L-132 cell line (B) Cells were exposed to Thuja occidentalis ethanolic extract for 24 h at different concentrations and the cell viability was determined by MTT assay. (jcimjournal.com)
Effects of Thuja occidentalis ethanolic extract on A549 cell migration (Light microscopy, ×20) Wound-healing assays were performed to assess cell migration. (jcimjournal.com)
The present study investigated the effects of Polyalthia longifolia leaf extract against the growth of HeLa cell xenograft tumor in nude mice and its underlying mechanism. (bvsalud.org)
The nude mice xenografted with HeLa cells were treated with 5% DMSO (vehicle control), 20 mg/kg/body weight of etoposide (positive control), and 500 and 1000 mg/kg/body weight of leaf extract, respectively. (bvsalud.org)
The extract also inhibits tumor angiogenesis by downregulating (p (bvsalud.org)
Conclusively, the P. longifolia leaf extract effectively inhibited HeLa cell xenograft growth in nude mice. (bvsalud.org)
Despite the well-known hypoglycemic, antiadipogenesis,, and antiobesity effects of constituents from Radix Paeoniae Rubra, the glucose-mediated insulinotropic effect of Radix Paeoniae Rubra extract and constituents on insulin secreting cells remained to be evaluated. (hindawi.com)
Therefore, we intend to answer these questions by testing the efficacy of Radix Paeoniae Rubra extract and/or constituents in cell-based platforms as well as in a mouse model of T2D. (hindawi.com)
17) have shown recently that a water-soluble extract of the adzuki beans could inhibit acetaminophen-induced liver damage. (healthjade.com)
In the same experimental system, IL-10 and BDNF gene expressions increased, and in HypoE22 cells, the extract decreased the extracellular dopamine level and increased the DAT gene expression due to the direct interaction of parthenolide with the DAT. (researchgate.net)

Furthermore, based on a GREM2 addition experiment, GREM2 has inhibitory effects on the adipogenesis of ASCs through activation of Wnt/β-catenin signaling. (fujita-hu.ac.jp)

Intersecting analysis, molecular docking, and pathway validation analysis showed that risperidone influences the adipocytokine signaling pathway by targeting MAPK14 (mitogen-activated protein kinase 14), MAPK8 (mitogen-activated protein kinase 8), and RXRA (retinoic acid receptor RXR-alpha), thereby inhibiting long-chain fatty acid β-oxidation by decreasing STAT3 (signal transducer and activator of transcription 3) expression and phosphorylation. (frontiersin.org)
Risperidone inhibits leptin-mediated phosphorylation of STAT3-Y705, leading to weight gain ( 26 ). (frontiersin.org)

We observed that 6,7,4'-THIF, but not daidzein, inhibited MDI-induced adipogenesis significantly at 40 and 80 μM, associated with decreased peroxisome proliferator-activated receptor-γ and C/EBP-α protein expression. (oregonstate.edu)

Mouse WIF1 expression vector (pCMV6-ENTRY-WIF1) was prepared, and used to transfect the mouse pre-adipocyte cell line 3T3-L1.The mRNA levels for WIF1, PPARγ and C/EBPα were then examined with real time RT-qPCR. (tnstate.edu)

We also showed that piceatannol directly binds to IR and inhibits IR kinase activity in a mixed noncompetitive manner to ATP, through which piceatannol appears to inhibit adipogenesis. (oregonstate.edu)

The 3T3-L1 Swiss sub clone line has been widely utilized, since its development, due to its affinity for lipid droplet deposition in vitro. (wikipedia.org)
Pre-clinical studies have shown antihypertensive and diuretic effects (5,6) and in vitro assays using culture cells revealed anti-adipogenic effects of T. majus extracts (7). (alies.pt)
The objective of this research is to examine the in vitro regulation of adipogenesis by WIF1. (tnstate.edu)

Here, we sought to determine the function of piceatannol in adipogenesis and elucidate the underlying mechanism. (oregonstate.edu)

Piperine reduces and stops cell death (anti-apoptotic) and is an anti-inflammatory, which plays a huge role in treating Parkinson's, a degenerative disease that mostly affects physical movement. (curejoy.com)
Black pepper has an anti-inflammatory effect that can reduce and prevent the creation of cancerous cells. (curejoy.com)
Its digestive benefits play a role in weight loss while its anti inflammatory properties can hinder the growth of fat cells. (ctohe.org)

This anti-adipogenic property of piceatannol was largely limited to the early event of adipogenesis. (oregonstate.edu)

Mechanistic studies shows that PLH activate Wnt/ß-catenin signaling pathway to inhibit the adipogenesis. (bvsalud.org)

DKK1 mRNA increases six hours after onset of human adipogenesis and this is followed by an increase in Dkk1 protein. (ox.ac.uk)
Results indicate that the transgene was expressed in the transfected cells within 30 hours after transfection, and the mRNA level of WNT target genes and CEBPα were affected. (tnstate.edu)
In conclusion, exogenous WIF1 was expressed in 3T3-L1 cells, at least at the mRNA level. (tnstate.edu)

Aside from the synthetic differentiation itself, 3T3-L1 lineages can display low differentiation efficiency when utilizing common differentiation methods. (wikipedia.org)
it is involved in the development of a wide variety of cell lineages and organs. (medscape.com)

Cell viability was ascertained through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay after deployment of TO in different doses. (jcimjournal.com)
TO demonstrated a dose-dependent decrease in viability of A549 cells after 24 h of exposure. (jcimjournal.com)

Recently, other groups demonstrated that a secreted antagonist of bone morphogenetic protein (BMP) signaling, Gremlin 2 (GREM2), inhibits the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) into osteoblasts and the adipogenesis of 3T3-L1 cell. (fujita-hu.ac.jp)

A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. (eurogentec.com)
Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. (eurogentec.com)
In addition, apigenin inhibited the DNA-binding activity of C/EBPβ in differentiating 3T3-L1 cells by down-regulating the 35 kDa isoform of C/EBPβ (liver-enriched activating protein) and up-regulating the expression of two different sets of C/EBP inhibitors: C/EBP homologous protein and the phospho-liver-enriched inhibitory protein isoform of C/EBPβ. (kist.re.kr)
The adzuki bean paste showed potential hypoglycemic activity in both normal mice and streptozotocin (STZ)-induced diabetic rats after an oral administration of sucrose, but did not show any effect on the blood glucose concentration after glucose administration, suggesting that the active fraction suppressed the postprandial blood glucose level by inhibiting alpha-glucosidase and alpha-amylase, irrespective of the endogenous blood insulin level. (healthjade.com)
Dose-dependent increase in glucose uptake activity (GUA) was observed in both cell lines. (wiley.com)
Melatonin inhibits aromatase activity and expression in human breast cancer cells, thus behaving as a selective estrogen enzyme modulator. (unican.es)
The presence of melatonin during differentiation also induced a parallel reduction in aromatase expression and activity and expression of the cells. (unican.es)

Yak was used as the experimental object to clone the yak CCAAT/Enhancer binding protein alpha( CEBPα ) gene, predicted its protein structure and function, isolated yak adipocyte, and detected the expression of CEBPα in different tissues of yak and yak fat expression during cell induction and differentiation. (hbnxb.net)

by inhibiting the hydrolysis of triglycerides, Orlistat reduces the absorption of fat from food to achieve weight loss. (frontiersin.org)
Black pepper reduces fat cell creation. (walkingoffpounds.com)

In this study, we evaluated the effects of HST on adipogenesis, its mechanisms of action and the molecular targets involved. (oregonstate.edu)

abstract = "Introduction: Adipose-derived stromal/stem cells (ASCs) have attracted attention as a promising material for regenerative medicine. (fujita-hu.ac.jp)
Abstract: Melatonin inhibits the genesis and growth of breast cancer by interfering at different levels in the estrogen-signaling pathways. (unican.es)

The cell apoptosis was determined by flow cytometry analysis. (jcimjournal.com)
MPP + can cause severe damage to mitochondrial morphology and the function in SH-SY5Y cells, and echinacoside selectively reverse mitochondrial function and cell apoptosis, yet there is no effect on mitochondrial morphology. (jcimjournal.com)
Fluorescence-activated cell sorting analysis after annexin V-fluorescein isothiocyanate and propidium iodide (annexin V-FITC-PI) dual staining method was done to ascertain early stage of apoptosis, if any. (jcimjournal.com)
Annexin-V-FITC positivity of cells up to 11.72% as compared to the untreated control revealed early state of TO-induced apoptosis. (jcimjournal.com)
The tumor tissue histological and immunohistochemical analyses showed that the HeLa tumor cell death was associated with apoptosis and decreased (p (bvsalud.org)

Black pepper and cardamom together can boost the strength of natural killer cells, thus showing anti-cancer ability. (curejoy.com)
By helping with natural detox, black pepper consequently prevents the growth of cancerous cells. (curejoy.com)

Finally, paeonol inhibits intestinal glucose absorption and provides antioxidant effect [ 15 , 16 ]. (hindawi.com)

During this period, cells can undergo hyperplastic growth until they are differentiated. (wikipedia.org)

In addition, to assess whether GREM2 affects adipogenesis, ASCs (purchased from a vendor) were cultured to induce adipogenesis with recombinant GREM2 protein, and siRNA-induced GREM2 knockdown experiment was also performed using aged ASCs. (fujita-hu.ac.jp)

Secretion of Wnts by adipose cells has an important role in the control of murine adipogenesis. (ox.ac.uk)
Finally, TCF7L2 polymorphisms have been associated with impaired insulin secretion, glucose production, and glucose tolerance via direct effects on pancreatic islet beta cells. (medscape.com)

In this study, we found that the SCARA5 expression was lower in CAL-27 and SCC-9 cells than that in human normal oral epithelial keratinocytes. (degruyter.com)
In addition, SCARA5 repressed OSCC cell epithelial-mesenchymal transformation (EMT), evidenced by increased E-cadherin expression and reduced N-cadherin expression. (degruyter.com)
The effects on dopamine (DA) release and dopamine transporter (DAT) gene expression were assayed in hypothalamic HypoE22 cells. (researchgate.net)
Since these events occur at a distance from the cell body, post-transcriptional control of gene expression has strategic advantage to fine-tune the overall regulation of protein contents in situ. (koreascience.kr)

The cells were cultured in a serum-containing medium and demonstrated a fibroblast-like morphology. (wikipedia.org)
Effects of echinacoside on MPP+-induced mitochondrial fragmentation in SH-SY5Y cells Mitochondrial morphology was visualized by laser confocal microscopy in live cell imaging system. (jcimjournal.com)

The 3T3 original cell line was isolated from a mouse embryo and propagated for 3this specific line of 3T3 cells is used to study adipose tissuerelated diseases and dysfunctions. (wikipedia.org)
These cells were originally used to study the properties of transformed cells and the mechanisms of neoplastic transformation. (wikipedia.org)
Taken together, our study reveals an anti-adipogenic function of piceatannol and highlights IR and its downstream insulin signaling as novel targets for piceatannol in the early phase of adipogenesis. (oregonstate.edu)
In a previous study, the RVD-hemopressin-α, an endogenous anorexigenic peptide, proved to be a negative allosteric modulator of CB1 [43] and to inhibit hypothalamic NE levels following peripheral administration despite being ineffective against DA and 5-HT levels [30]. (researchgate.net)

These cells will be identified as 3T3 only after they are taken through 20 to 30 passages and have established at a stable growth rate. (wikipedia.org)

Basal cell culture medias for 3T3-L1 cells tends to contain a version of Dubelcco's Modified Eagle's Medium (DMEM), fetal bovine serum (FBS) , and an antibiotic at specific levels dependent upon protocol. (wikipedia.org)

If you have lower insulin resistance, your cells will require less insulin to clear your blook of excess glucose or blood sugar. (walkingoffpounds.com)
Hyperglycemia in T2D patients is the consequence of both insulin resistance and beta-cell dysfunction or failure [ 2 ]. (hindawi.com)

17, 18] TCF7L2 protein is increased during adipogenesis in 3T3-L1 cells and in primary adipocyte stem cells. (medscape.com)

The Wnt antagonist Dickkopf-1 and its receptors are coordinately regulated during early human adipogenesis. (ox.ac.uk)

Anatomy17

Organisms3

Diseases3

Chemicals and Drugs22

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes11

Preadipocytes17

Adipocytes12

Mitotic clonal expansion5

Clonal expansion2

Lipid accumulation3

Accumulation1

Adipocyte differentiation1

Proliferation6

Inhibition1

PI3K1

Concomitant1

Obesity5

Dose-dependently1

Extract12

Inhibitory Effects1

Phosphorylation2

Peroxisome1

PPAR1

Kinase activity1

Vitro3

Elucidate1

Anti-inflammatory3

Largely limited to the early1

Pathway1

MRNA3

Lineages2

Viability2

Mesenchymal stem1

Activity7

CCAAT1

Reduces2

Mechanisms of act1

Abstract2

Apoptosis5

Black pepper2

Intestinal1

Undergo1

Induce1

Secretion2

Expression4

Morphology2

Study4

Stable1

Levels1

Insulin resistance2

Protein1

Early1