Inhibits adhesion adhesion laminin. Medical search. Frequent questions

Cells expressing stem cell markers, including GFAP, and proliferation markers are closely apposed to the laminin-containing extracellular matrix (ECM) surrounding vascular endothelial cells. (nih.gov)
Functional analyses indicated that some of the genes induced by dragonfly larvae caused an increase in laminins necessary for cell adhesion in the extracellular matrix. (biomedcentral.com)
Indeed, granule cells from the rostral region of perinatal Gpr56 −/− cerebella show loss of adhesion to extracellular matrix molecules of the pial basement membrane. (jneurosci.org)
Consistent with this hypothesis, developing granule cells from the rostral region of Gpr56 −/− cerebella at birth exhibit a specific defect in cell adhesion to extracellular matrix (ECM) molecules of the pial basement membrane (BM) but no defects in migration, proliferation, or process outgrowth. (jneurosci.org)
Extracellular matrix is a fibrillar network composed of structural (collagens) and adhesion (fibronectin) proteins crucial for tissue architecture and biological pathway Histol Histopathol 27:1515-1528, 2012 . (springer.com)
We observed alteration in biological functions related to extracellular matrix function and organization, cellular adhesion, muscle growth, lipid metabolism and proteolysis. (biomedcentral.com)
8,9 The underlying stromal matrix contains proteins that suppress transforming growth factor (TGF)-β signaling in fibroblasts, and consequently inhibit the proliferation and differentiation into myofibroblasts, extracellular matrix production, and scar formation. (touchophthalmology.com)
The laminin globular (G) domain can be found in one to several copies in various laminin family members, including a large number of extracellular proteins. (embl.de)
Merging extracellular domains: fold prediction for laminin G-like and amino-terminal thrombospondin-like modules based on homology to pentraxins. (embl.de)

In addition, solutions of these peptides or antibodies generated against these peptides inhibited laminin-mediated HT-1080 cell adhesion. (rupress.org)
Antibodies against the beta 1 integrin subunit inhibited HT-1080 cell adhesion and neurite outgrowth on surfaces adsorbed with peptides GD-3 and GD-4. (rupress.org)
Adherence was inhibited by antibodies to laminin and laminin receptor and was calcium ion and temperature dependent. (aai.org)
Disruption of GalTase adhesion by exposure to anti-GalTase antibodies during this period resulted in extension of the G2 phase, a result similar to that seen with agents known to inhibit growth and induce differentiation. (tmc.edu)
C) Inhibitory effects of anti-Lu/B-CAM antibodies on Lu-Fc binding to laminin α5. (bio-rad-antibodies.com)
Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)
Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)
Laminin-332 polypeptides are targeted by auto-antibodies in human patients with mucous membrane (cicatricial) pemphigoid or, more rarely, subepidermal vesicular diseases that resemble epidermolysis bullosa acquisita (EBA) or bullous pemphigoid (BP). (morulaivf.com)
In all nine dogs, IgG auto-antibodies detected purified human laminin-332 by immunoblotting. (morulaivf.com)

Adult SVZ progenitor cells express the laminin receptor alpha6beta1 integrin, and blocking this inhibits their adhesion to endothelial cells, altering their position and proliferation in vivo, indicating that it plays a functional role in binding SVZ stem cells within the vascular niche. (nih.gov)
The ability of lovastatin, mevastatin and simvastatin to inhibit the melanoma cell proliferation was examined using cytotoxicity and apoptosis assays. (biomedcentral.com)
The concentrations required to inhibit proliferation of melanoma cells (0.8-2.1 μM) have previously been achieved in a phase I clinical trial of lovastatin in patients with solid tumours, (45 mg/kg/day resulted in peak plasma concentrations of approximately 3.9 μM). (biomedcentral.com)
Some of the most enriched biological processes were lymphocyte activation and proliferation, leukocyte adhesion and migration, antigen processing and presentation and cellular response to IFN-γ. (sciencegate.app)
High‐salt exposure also inhibited cell proliferation and promoted apoptosis. (uea.ac.uk)
Signals emanating from focal adhesions have been shown to promote survival, differentiation and proliferation [ 5 ]. (thno.org)
It affects cell proliferation, differentiation and inhibits apoptosis, and its role in tumor development has received attention ( 9 ). (spandidos-publications.com)
Overexpression of IGF-1 in serum and tissue alters the growth of normal cells and causes uncontrolled proliferation, inhibits differentiation and reduces apoptosis, resulting in the incidence and development of malignant tumors ( 10 ). (spandidos-publications.com)
4,19-21 The immunosuppressive ability of AM to inhibit a mixed lymphocyte reaction appears to be partly mediated by a soluble factor, resulting in decreased alloreactive T-cell proliferation and Th1 and Th2 cytokine production. (touchophthalmology.com)

The object of this work has been to investigate the formation and function of GalTase-specific adhesions during F9 cell growth and differentiation. (tmc.edu)
Finally, other studies have suggested that a subset of cell adhesion molecules have the capability to induce differentiation in EC cells systems. (tmc.edu)
We have determined in F9 cells that dissociating GalTase adhesion by galactosylation of and release of the consociate glycoproteins induces differentiation, as defined by increased laminin synthesis. (tmc.edu)
The ability to induce differentiation by surface galactosylation was greatest in cells grown in cultures promoting cell-cell adhesions, relative to cultures with minimal cell-cell interactions. (tmc.edu)
2-5 The basement membrane provides a substrate that promotes epithelial cell adhesion, migration, and differentiation 5-7 and prevents epithelial cell apoptosis. (touchophthalmology.com)
Laminin G-containing proteins appear to have a wide variety of roles in cell adhesion, signalling, migration, assembly and differentiation. (embl.de)

Synthetic peptides from the carboxy-terminal globular domain of the A chain of laminin: their ability to promote cell adhesion and neurite outgrowth, and interact with heparin and the beta 1 integrin subunit. (rupress.org)
In experiments probing for a role of PKCa in human laminin-2-integrin-mediated cell adhesion and spreading of PC12 cells, we observed unexpected enhancements of adhesion, spreading and stress fiber formation to 1 mM Go¨6976 with concomitant increase in membrane translocation of PKCd and autophosphorylation of focal adhesion kinase (FAK). (mirnamimic.com)
Biological activities of laminin are mediated through binding to a subset of integrin family of cell adhesion receptors, a3b1, a6b1, a6b4, and a7b1 [2]. (mirnamimic.com)
In PC12 cells, we recently showed that the engagement of integrin a3b1 to a binding sequence within the human laminin a2 chain was sufficient to recruit PKCa and PKCd to the integrin receptor [6]. (mirnamimic.com)
Integrin mediated cellular adhesion is dynamic process that is both indicative of and influenced by cell status. (thno.org)
The integrin tails have no intrinsic kinase activity but rather serve as a site for the docking of various kinases and related adaptor proteins that comprise focal adhesions. (thno.org)

It constitutes fibrous proteins such as collagen and elastin, elongated glycoproteins such as fibronectin and laminin, which provide cell matrix adhesion. (biomedcentral.com)

A specific inhibitor of PKCd, rottlerin, completely abrogated Go¨6976-dependent increase in PC12 cell adhesion to laminin as well as the activation of small GTPases, Rac1 and Cdc42, that are downstream of PKCd in adhesion receptor signaling. (mirnamimic.com)
P-selectin, soluble endothelial protein C receptor, soluble thrombomodulin, plasminogen activator inhibitor 1, ADAMTS-13, von Willebrand factor, tissue factor, soluble intercellular adhesion molecule 1, and vascular cell adhesion molecule 1 were more elevated in LF patients than in controls. (cdc.gov)
Endothelial protein C receptor, thrombomodulin, intercellular adhesion molecule 1, plasminogen activator inhibitor 1, D-dimer, and hepatocyte growth factor were higher in fatal than nonfatal LF cases. (cdc.gov)

ELAV - like1 , methyltransferase like 7A , dolichyl-phosphate mannosyltransferase , laminin subunit beta-1 , gremlin 1 , BCL6 corepressor-like 1 , and three genes of unknown identity. (biomedcentral.com)

Laminin binding proteins. (am-online.org)
This fibrillar network is composed of many structural proteins, such as collagens, and adhesion proteins, including fibronectin and proteoglycans (perlecan, decorin) [ 13 , 14 ]. (springer.com)
14-17 Vascularization is inhibited by the antiinflammatory effect of AM and by the antiangiogenic proteins present within AM, including endostatin, thrombospondin-1, and inhibitors of metallo-proteases. (touchophthalmology.com)

Laminins are multifunctional basement membrane glycoproteins composed of a, b, and g polypeptide chains. (mirnamimic.com)

Endogenous laminin α5 and bound Lu-Fc were detected with anti-laminin α5 domain LEb/L4b and anti-human IgG, as indicated. (bio-rad-antibodies.com)
The names 'acquired junctional epidermolysis bullosa', 'anti-laminin-332 mucous membrane pemphigoid (MMP)' and 'mixed auto-immune subepidermal blistering dermatosis' are proposed for dogs with clinical signs reminiscent of EBA, MMP or BP respectively. (morulaivf.com)

Merged pictures showed that laminin α5 and Lu-Fc co-localized at basement membranes. (bio-rad-antibodies.com)
Here we report high‐salt exposure inhibited basement membrane breakdown by affecting RhoA, thus disturbing the expression of Slug/E‐cadherin/N‐cadherin/Laminin and interfering with mesoderm formation during the epithelial‐mesenchymal transition(EMT). (uea.ac.uk)
Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)
In five dogs, IgG variably recognized the basement membrane of laminin-332-deficient human skin (three dogs negative, two dogs positive). (morulaivf.com)
These observations establish laminin-332 as a novel basement membrane antigen in dogs with autoimmune blistering diseases with variable clinical phenotypes. (morulaivf.com)
The AM basement membrane contains laminin, fibronectin, and collagen type IV and VII, similar to the basement membrane of conjunctival tissue, but more than corneal epithelium. (touchophthalmology.com)

Lovastatin, mevastatin and simvastatin inhibited the growth, cell migration and invasion of HT144, M14 and SK-MEL-28 melanoma cells. (biomedcentral.com)
Synthetic peptides or recombinant fragments containing bio- logically active motifs have been shown to partially mimic the effects of native laminin in various cellular functions, such as cell adhesion, spreading, migration, neurite outgrowth, and tumor metastasis [6-10], suggesting that laminin-derived-short peptide- modified biomaterials may have therapeutic potential in tissue regeneration applications. (mirnamimic.com)
PKC activation by phorbol myristate acetate has been shown to induce the adhesion, spreading, and migration of cells [12]. (mirnamimic.com)
Furthermore, the DiI+ cell migration trajectory in vivo and scratch wound assays in vitro indicated that high‐salt exposure restricted cell migration of cardiac progenitors, which was caused by the weaker cytoskeleton structure and unaltered corresponding adhesion junctions at HH7. (uea.ac.uk)
Bioactive phospholipids enhance migration and adhesion of human leukemic cells by inhibiting Heme oxygenase 1 (HO-1) and inducible nitric oxygenase synthase (iNOS) in a p38 MAPK-dependent manner. (livedna.net)

Treatment of cells with dibutyryl cAMP, which activates protein kinase A, inhibited both adherence and histamine release induced by Ag or calcium ionophore. (aai.org)
Treatment of cells with staurosporin, which inhibits protein kinase C, also inhibited adherence and histamine release induced by calcium ionophore, but was not significantly active against either adherence or histamine release induced by Ag. (aai.org)
Uvomorulin, lysosome-associated membrane protein-1 (LAMP-1), and laminin were recognized by surface GalTase and are, therefore, potential components in GalTase-specific adhesions. (tmc.edu)
To the most important virulence factors belong: capsule, C5a peptidase, which inhibits the action of neutrophils, α-C protein, laminin binding protein, and β hemolysin typical for invasive strains. (am-online.org)
Additionally, GBS strains may present FbsA protein which protects bacteria from opsonization and fagocytosis as well as takes part in adhesion. (am-online.org)
Important biological events associated with plasma membranes, such as signal transduction, cell adhesion, and protein trafficking, are mediated through the membrane microdomains. (mdpi.com)
Using a new method for construction and database searches of sequence consensus strings, we have identified a new superfamily of protein modules comprising laminin G, thrombospondin N and the pentraxin families. (embl.de)
Induction of an active model of autoimmunity against type VII collagen in a hairless mouse strain (SKH1) revealed that regulatory T cells, which have been identified for their ability to inhibit the development of autoimmune diseases, do not inhibit the development of autoantibodies against the self-protein mouse-type VII collagen. (medscape.com)

Coating of glass slides with Entactin-Collagen IV-Laminin attachment (ECL) matrix further enhanced the adhesion of P. multocida. (usda.gov)
Immunological investigations consisted of direct immunofluorescence (IF), indirect IF with intact and salt-split canine gingival, and salt-split normal or laminin-332-deficient human skin, immunoblotting with purified human laminin-332 and immunoblotting with recombinant NC1 domain of human collagen VII. (morulaivf.com)
Autoantibodies specific for type VII collagen alter the dermoepidermal junctional adhesion and lead to dermoepidermal separation. (medscape.com)

The mechanical kinetics of adsorption have also been examined by testing the adhesion strength of an individual fibrinogen molecule that is affixed to an AFM tip and is briefly touched to a silica surface [ 566 ]. (nanomedicine.com)
The internal surfaces of cellulose acetate hollow fibres, of comparable internal diameter to small blood vessels, were coated with normal serum human IgG, heat-aggregated IgG (HAIgG), laminin or fibrinogen. (dundee.ac.uk)

residues 2,110-3,060) of the A chain of murine-derived laminin has been shown to promote heparin binding, cell adhesion, and neurite outgrowth. (rupress.org)

Therefore, laminin appears to have multiple, independent sequences in the G domain that serve a similar cell adhesion promoting function for different cell types. (rupress.org)
Hence, the high-affinity IgE-R on the mast cell functions not only in exocytosis but also facilitates the process of mast cell adherence to laminin. (aai.org)
Galactosyltransferase (GalTase) is localized in the Golgi, where it functions in oligosaccharide synthesis, as well as on the cell surface where it serves as a cell adhesion molecule. (tmc.edu)
GalTase-specific adhesions are functional in a number of important biological events, including F9 embryonal carcinoma (EC) cell adhesions. (tmc.edu)
GalTase-based adhesions are formed by recognition and binding to terminal N-acetylglucosamine (GlcNAc) residues on its glycoprotein counterpart on adjacent cell surfaces. (tmc.edu)
These data indicated that the increase in cell adhesions expected with increased cell-matrix interaction in differentiated F9 cells is not the consequence of increased surface GalTase expression and, more interestingly, that the two pools of GalTase are under differential regulation. (tmc.edu)
Furthermore, these interactions were aberrant in an adhesion-defective F9 cell line that results, at least in part, from abnormal oligosaccharide synthesis. (tmc.edu)
Inhibition of surface GalTase function by a panel of reagents inhibited F9 cell growth. (tmc.edu)
Go¨6976 also induced laminin-dependent cell adhesion in NIH/3T3 and CV-1 fibroblasts, suggesting of a mechanism that may be common to multiple cell-types. (mirnamimic.com)
siRNA knockdown of Rac1 and Cdc42 expression inhibited cell spreading and lamellipodia formation in PC12 cells. (mirnamimic.com)
Pharmacologic blockade of PKC inhibits cell adhesion and spreading as well as focal adhesion formation and focal adhesion kinase (FAK) phosphorylation [13,14]. (mirnamimic.com)
In preliminary experiments examining the role of PKCa in cell adhesion and spreading, we found that Go¨6976, a widely used, nonglycosidic indolocarbazole inhibitor of PKCa/b, had effects that could not be explained by inhibition of PKCa/b alone. (mirnamimic.com)
cancer cell growth and spreading, it has also been proposed that GPR56 regulates cell adhesion, but this has remained untested in the nervous system. (jneurosci.org)
This adhesion was similarly inhibited by excess soluble IgG, confirming the capability of FcγR to mediate cell arrest. (dundee.ac.uk)
Decorin inhibits cell attachment to thrombospondin-1 by binding to a KKTR-dependent cell adhesive site present within the N-terminal domain of thrombospondin-1. (embl.de)
1992]. Molecular mechanisms by which DCN interacts with TSP and inhibits cell adhesion to TSP are unknown. (embl.de)

Integrins are heterodimeric, transmembrane receptors that function as mechanosensors, adhesion molecules and signal transduction platforms in a multitude of biological processes. (thno.org)

Lay Summary The JAK/STAT inhibitor tofacitinib, recently approved for the treatment of ulcerative colitis, is found to modulate the intestinal endothelial barrier functions in directing the leukocyte adhesion and transmigration in ulcerative colitis patients displaying high levels of endothelial STAT3/STAT6 phosphorylation. (sciencegate.app)

Transfection of 10 nM CTGF siRNA inhibited the TGF-β2-induced upregulation of CTGF, Col4α2, Col1α1, TGM2, and FN, whereas TSP-1 expression was not reduced. (arvojournals.org)

With serogroup A, capsular hyaluronic acid (HA) promotes adhesion to elicited turkey air sac macrophages (TASM). (usda.gov)

These studies establish a novel role for GPR56 in the adhesion of developing neurons to basal lamina molecules and suggest that this adhesion is critical for maintenance of the pia and proper cerebellar morphogenesis. (jneurosci.org)

These three stages of apposition, attachment, and invasion are also alternatively termed contact (apposition), adhesion (attachment), and penetration (invasion), and can only take place during a limited timeframe known as the window of implantation when the uterus is at its most receptive. (wikipedia.org)

Addition of hyaluronic acid to the TPBM cultures inhibited bacterial adherence similar to that previously observed for TASM. (usda.gov)
however, culture of mast cells with TGF-beta 1 was determined to enhance IgE-mediated adherence to laminin. (aai.org)

Arrest on HAIgG was inhibited by excess soluble IgG but not by bovine serum albumin (BSA), demonstrating that the adhesion was IgG-specific and presumably mediated by FcγR on the PMN surface. (dundee.ac.uk)

However, higher doses of statins may have a role to play in adjuvant therapy by inhibiting growth and invasion of melanoma cells. (biomedcentral.com)

Skin decorin (DCN) is an antiadhesive dermatan sulfate-rich proteoglycan that interacts with thrombospondin-1 (TSP) and inhibits fibroblast adhesion to TSP [Winnemoller et al. (embl.de)

It might have an important polymorphic association at the promoter regions of several MMPs such as MMP-1 (-1607 1G/2G), MMP-2 (-1306 C/T), MMP-3 (-1171 5A/6A), MMP-9 (-1562 C/T) and TIMP-2 (-418 G/C or C/C). Tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring inhibitors of MMPs, which inhibit the activity of MMPs and control the breakdown of ECM. (biomedcentral.com)
Tissue inhibitors of matrix metallo-proteinases (TIMPs) are known to have the ability to inhibit the catalytic activity of MMPs. (biomedcentral.com)

Regulation of adhesion of mouse bone marrow-derived mast cells to laminin. (aai.org)
We have reported that mast cells adhere to laminin after activation with PMA. (aai.org)
Interference RNA-mediated knockdown of GPR56 recapitulates the loss of adhesion seen in knock-outs, and reexpression of GPR56 rescues the adhesion defect in knock-out granule cells. (jneurosci.org)
These results support a novel hypothesis that the cerebellar defects in Gpr56 knock-outs, and potentially in human patients with GPR56 mutations, arise from the disrupted adhesion of developing granule cells to the pial BM. (jneurosci.org)
However, the subsequent spreading process of these cells was found to be inhibited for at least 2 h on a laminin substratum. (morulaivf.com)
Spreading-arrested and rounded CEF do not develop prominent intracellular stress-fibers like control cells, instead they seem to concentrate their available actin in areas of presumptive initial contact with the laminin substratum. (morulaivf.com)
Mesenchymal stem cells with IGF-1 and TGF-β1 in laminin gel for osteochondral defects in rabbits. (livedna.net)

B) Binding of Lu-Fc to laminin α5 in adult mouse skeletal muscle sections. (bio-rad-antibodies.com)

This study was conducted to define the potential sequence(s) originating from the G domain of laminin with any of these functional activities. (rupress.org)

Upon vascular injury, platelets are exposed to the subendothelium, and several agonists, including adenosine diphosphate (ADP) and thrombin, are generated at the injury site, which can stimulate platelet adhesion, activation and aggregation. (spandidos-publications.com)

Adhesion and penetration. (am-online.org)

In vitro maturation of TPBM over a period of 6 days resulted in gradual increased adhesion of the bacteria. (usda.gov)
In vitro assays demonstrated a competition of laminin and this monoclonal antibody for the binding site on purified 5′-nucleotidase. (morulaivf.com)

The β-tail is the primary docking site in the formation of focal adhesions, but the alpha tail in some cases may also serve as a signaling scaffold for calcium dependent signaling events. (thno.org)

Mouse anti Human CD239 antibody, clone BRIC221 ( MCA1982 ) used to look for blocking of Lu-Fc to endogenous α5 laminin in kidney sections. (bio-rad-antibodies.com)

Treatment of PMN with the regulatory neuropeptide substance P also enhanced adhesion to HAIgG-coated fibres and caused increased surface expression of FcγRI, FcγRII and FcγRIII. (dundee.ac.uk)

If you choose to take turmeric/curcumin (traditionally, curcumin has been much more widely recognized, but newer research suggests the whole herb has more advantages), look for a product that contains piperine or Bioperine (black pepper extract), which inhibits the enzymes that break down most supplements and medicines in the gut. (blogspot.com)

In conclusion, the data presented here demonstrated that LGP, a natural compound from C. nudiflora Hook, inhibited the development of platelet aggregation and amplification of platelet activation. (spandidos-publications.com)

Addition of QSYQ protected the downregulated ATP 5D and upregulated p-Src and Caveolin-1 after tPA treatment.Conclusion: Our results show that QSYQ inhibits tPA-induced brain edema and hemorrhage by protecting the blood-brain barrier integrity, which was partly attributable to restoration of energy metabolism, protection of inflammation and Src/Caveolin signaling activation. (sciencegate.app)

Anatomy39

Organisms7

Diseases8

Chemicals and Drugs123

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes50

Disciplines and Occupations2

Technology, Industry, Agriculture4

Information Science4

Extracellular9

Antibodies10

Proliferation9

Differentiation6

Integrin6

Fibronectin and laminin1

Receptor3

Subunit1

Proteins3

Glycoproteins1

Anti-laminin2

Basement6

Migration5

Protein8

Collagen3

Fibrinogen2

Outgrowth1

Cell16

Signal transduction1

Endothelial1

SiRNA1

Promotes1

Molecules1

Attachment1

Adherence2

Soluble1

Invasion1

Decorin1

MMPs2

Cells7

Skeletal1

Functional1

Vascular1

Penetration1

Vitro2

Dependent1

Human1

Expression1

Extract1

Development1

Activation1