• Adenine induced significant apoptosis in HT29 cells, whereas Caco-2 cells exhibited less apoptotic responses. (hindawi.com)
  • Inhibition of cell viability was assessed with focus on apoptosis transcriptionally and translationally. (hindawi.com)
  • Chronic myeloid leukemia (CML) results from BCR-ABL oncogene, which blocks CML cells differentiation and protects these cells from apoptosis. (jcancer.org)
  • Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
  • Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
  • In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
  • Induction of apoptosis in human leukaemic cell lines K562, HL60 and U937 by diethylhexylphthalate isolated from Aloe vera Linne. (tripod.com)
  • We investigated the effect of diethylhexylphthalate (DEHP) from Aloe vera Linne on the apoptosis of human leukaemic cell lines K562, HL60 and U937 to examine its pharmacological activity. (tripod.com)
  • The cytotoxicity mechanism consists of the induction of apoptosis, whereas the selectivity against neuroectodermal tumor cells is founded on a specific energy-dependent pathway of drug incorporation. (tripod.com)
  • Growth of cancer cells is regulated by the balance between cell proliferation and apoptosis, which is a normal physiological process serving to eliminate unwanted cells and maintain homeostasis in healthy tissue. (nutraculture.com)
  • Nutraculture™ Phycocyanin™ induces apoptosis in the existing and proliferating cancer cells and being a natural antioxidant, it also helps getting rid of any cancer promoting oxidative stress. (nutraculture.com)
  • It is established that Phycocyanin induces apoptosis in cancer cells by changing the Bcl-2/Bax ratio (Bcl-2 is an anti apoptotic protein, Bax is a pro-apoptotic protein, the ratio of Bcl-2/Bax represents the degree of apoptosis) and the release of Cyt-c in the cytosol (Lu et. (nutraculture.com)
  • The cell apoptosis was detected using flow cytometry. (lookformedical.com)
  • If this had been the situation, the broken Atm_/_ cells would fail to engage G1 arrest and progress by means of S-phase, major alternatively to Atr-dependent stalled replication forks and an increase in p53-dependent apoptosis in lieu of DNA repair. (cd31-signal.com)
  • AVM induced the release of cleaved PARP and cleaved caspase‑3 caused apoptosis and inhibited the viability of these cells. (spandidos-publications.com)
  • This clinical cancer strategy incorporates advancements in cancer treatment based on a multi-approach to the disease that includes targeting apoptosis, immune cell activation, angiogenesis, restoration of cellular respiration, inhibition of inflammatory mediators, not mentioning some secondary factors such as intoxication, disturbed microbiome, and nervous disorders. (biobran.org)
  • After 72 h incubation, colorimetric 3-[4,5-dimethylthiazol-2- yl]-2,5 diphenyl tetrazolium bromide test was performed to determine half-maximal inhibitory concentration values of each compound. (ijpsonline.com)
  • In recent studies, this linker modified analog of goniothalamin was shown to be cytotoxic against PC-3 (prostate cancer) and MCF-7 (human breast cancer) cell lines with half maximal inhibitory concentration values of 0.13 μM and 2.6 μM, respectively. (lookformedical.com)
  • HUVEC when comparing the IC50 (half maximal inhibitory concentration) values. (lookformedical.com)
  • SETD2 loss causes growth arrest and differentiation of AML cells, and leads to increased DNA damage. (nature.com)
  • Expression of MLL-fusions enhances proliferation and blocks myeloid differentiation of hematopoietic progenitor cells, leading to their pathological accumulation. (nature.com)
  • First, we show herein that activin A induced the expression and activated the promoters of α-globin and ζ-globin in K562 cells, confirming that activin A induces erythroid differentiation in K562 cells. (tmu.edu.tw)
  • We conclude that activin A can regulate the same pathway via p38 MAPK to coordinate cell proliferation and differentiation of K562 cells. (tmu.edu.tw)
  • We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
  • Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
  • JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)
  • BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)
  • Cancer is the uncontrolled growth of cells in the body, which is caused by the stepwise accumulation of mutations that affect cell growth control, differentiation and survival. (nutraculture.com)
  • RNAi knock down of kaiso in K562 cells block hematopoietic differentiation. (srcsignaling.com)
  • These data demonstrate that African green monkey-like natural killer cell differentiation profiles can be rescued in rhesus macaques to promote viral clearance in tissues. (nature.com)
  • define NK cell differentiation states based on their education via NKG2a and expression of CD16 (FcγRIII), an activating Fc receptor that mediates antibody-dependent cell-mediated cytotoxicity (ADCC) 8 , and demonstrate that nonpathogenic SIVagm infection in African green monkeys (AGMs) imprints the maturation of NK cells inducing terminally differentiated NKG2a low CD16 + NK cells, which express high levels of interleukin (IL)−21R. (nature.com)
  • Cell viability was measured using the MTT assay. (hindawi.com)
  • Second, SB203580 inhibited the inhibitory effect of activin A on the colony-forming activity of K562 cells using the methylcellulose colony assay, indicating that activin A inhibits K562 colony formation by activating p38 MAPK. (tmu.edu.tw)
  • So extract exhibited a significant inhibitory activity in CAM assay in a dose dependent manner. (celljournal.org)
  • Antiproliferative effects of compounds were determined using carboxyfluorescein succinimidyl ester assay. (ijpsonline.com)
  • At a level of 10 microg mL(-1) DEHP a significant anti-leukaemic effect was observed for all three cell lines, as measured by clonogenic assay. (tripod.com)
  • Because Kaiso is thought of a methylation dependent op portunistic oncogene, it had been conceivable to discover the biological purpose of Kaiso over the cells growth in vitro, the pro liferation of K562 cells was evaluated by a WST one assay. (srcsignaling.com)
  • The half-maximal inhibitory concentration (IC50) of Cd and the safe doses of SFN were determined using a methyl thiazolyl tetrazolium (MTT) assay. (lookformedical.com)
  • The testosterone secretion from TM3 cells was measured using the enzyme-linked immunosorbent assay. (lookformedical.com)
  • The active antimicrobial derivatives were further evaluated for cytotoxicity against human lung carcinoma cell line (HCT-116), breast cancer cell line (MDAMB231), leukemic cancer cell line (K562), and normal cell line (HEK293) by MTT assay using doxorubicin as the standard drug. (biomedcentral.com)
  • We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)
  • The expanded NK cells were significantly more potent than unstimulated or IL-2-stimulated NK cells against acute myeloid leukemia cells in vitro . (aacrjournals.org)
  • The isolated compound from Aloe vera Linne, DEHP, was considered to be the active principle responsible for anti-leukaemic and anti-mutagenic effects in-vitro. (tripod.com)
  • Nevertheless, the p120ctn knock down alone showed a reduce by 65% in B catenin ranges whilst the Kaiso p120ctn double knock down line didn't substantially affect B catenin levels in vitro when compared to scrambled knock down cells. (srcsignaling.com)
  • This considerable boost in SCF expression correlated with a rise on in vitro cell proliferation. (srcsignaling.com)
  • Fetal equine dermal (Given) cells are vunerable to EIAV in vitro, as well as the replication of EIAV in Given cells transiently transfected with ELR1-IN was markedly decreased in comparison to replication in cells transfected using the unfilled vector. (angiogenesis-blog.com)
  • This article summarizes recent advances in in vitro and in vivo research on the anti-cancer effects and related mechanisms of some promising natural products. (anti-agingfirewalls.com)
  • NK cells cytokine expression, signaling and cytotoxicity has been evaluated in vitro. (bmj.com)
  • an ATP-binding ), coumarins () and terpenoids cassette (ABC) transporter (150-170 kDa), which pumps out lipo- have been reported as philic agents from cells that have entered them by free diffusion agents for overcoming multidrug resistance, and could be used aloneat very low concentrations or in combination to reverse multidrugresistance in vitro. (gotomydoctor.com)
  • No consistent effects were observed for NK number in blood (mean difference [MD]: 1.47, 95% confidence interval [CI] − 0.35 to 3.29, p = 0.113) or NKCA as assessed in vitro (MD: − 0.02, 95%CI − 0.17 to 0.14, p = 0.834). (springeropen.com)
  • Current evidence does not support a significant effect of exercise training intervention on NK cells in blood or on their 'static response' (as assessed in vitro) in cancer survivors. (springeropen.com)
  • Pixantrone (0.01-10 μM) shows potent inhibitory activities against rat 97-116 peptide-specific T cell proliferation [4] . (fluoroprobe.com)
  • Our findings suggest that adenine inhibits the growth of colon cancer cells. (hindawi.com)
  • However, the mechanism by which adenine inhibits the proliferation of cancer cells is sketchy. (hindawi.com)
  • Huang, HM , Chiou, HY & Chang, JL 2006, ' Activin A induces erythroid gene expressions and inhibits mitogenic cytokine-mediated K562 colony formation by activating p38 MAPK ', Journal of Cellular Biochemistry , vol. 98, no. 4, pp. 789-797. (tmu.edu.tw)
  • and the cytokine TGF-β1 secreted by NK cells further inhibits T cells proliferation. (zjujournals.com)
  • Pixantrone potently inhibits growth of human Leukemia K562 cells, etoposide-resistant K/VP.5 cells, MDCK and ABCB1-transfected MDCK/MDR cells, with IC 50 s of 0.10 μM, 0.56 μM, 0.058 μM and 4.5 μM, respectively. (fluoroprobe.com)
  • Taken jointly, our data suggest that sELR1 serves as a secreted mobile aspect that inhibits EIAV an infection in web host cells. (angiogenesis-blog.com)
  • FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling. (cancerindex.org)
  • Inhibits CD25 translation through regulation of the LKB1-AMPK-mTOR pathway to suppress T cells. (cusabio.com)
  • Adenine has been suggested to induce cell cycle arrest in cancer cells, leading to cell death [ 9 , 10 ]. (hindawi.com)
  • In addition, mitogenic cytokines SCF, IL-3, and GM-CSF induced colony formation of K562 cells that could be inhibited by PD98059 or enhanced by SB203580, respectively, indicating that these mitogenic cytokines induce K562 colony formation by activating ERK and inactivating p38 MAPK. (tmu.edu.tw)
  • Age-related and metabolic diseases such as type 2 diabetes (T2D) represent a source of cellular stress due to their disruptive effect on normal physiological processes and, therefore, can induce premature senescence ( 10 ). (frontiersin.org)
  • Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)
  • Objective: To investigate the effect of blocking the inhibitory receptors KIR2DL1 and KIR2DL2/2DL3 with monoclonal antibody on cytotoxic activity of human NK cells. (zjujournals.com)
  • Both BLM and A5 are hydrophilic molecules that depend on transporters or endocytosis receptors to get inside of cells. (lookformedical.com)
  • Introduction For some retroviruses, the viral envelope binds to receptors within a pH-independent way, recommending which the virions may fuse towards the cell membrane [1] straight. (angiogenesis-blog.com)
  • As a result, viral receptors over the cell membrane offer binding sites for the trojan and so are also mixed up in structural modulation of viral envelopes, resulting in the fusion Pseudouridimycin from the viral and mobile membranes and virion entrance, the first step in viral an infection of focus on cells [2]. (angiogenesis-blog.com)
  • As opposed to almost every other lentiviruses, such as for example human immunodeficiency trojan (HIV)-1, simian immunodeficiency trojan (SIV) and feline immunodeficiency trojan (FIV), which need co-receptors for effective an infection, EIAV seems to depend just on an operating ELR1 for the invasion of focus on cells. (angiogenesis-blog.com)
  • NK cells recognize the targets through the biased signals transmitted from active or inhibitory receptors. (biomedcentral.com)
  • In the last years higher attention focused on the role of immunoregulation both in the insulin production by β-cell of Langerhans-islets and in the insulin sensitivity (resistance) of insulin receptors. (biobran.org)
  • As observing in this study, SOextract could inhibit proliferation of the different cells at the concentrations above 200 μg/ml without toxic effect on the cells in doses ranged from 0-500 μg/ml. (celljournal.org)
  • It is of interest to explore the inhibitory effect of adenine on the growth of colorectal cancer cells and to determine the underlying mechanism. (hindawi.com)
  • These mice are devoid of endogenous natural killer and T-cell activity and were used to determine whether adoptively transferred expanded natural killer cells could inhibit myeloma growth and myeloma-associated bone destruction. (haematologica.org)
  • Adoptive transfer of expanded natural killer cells inhibited the growth of established OPM2 and high-risk primary myeloma tumors grown in the murine model. (haematologica.org)
  • The transferred, expanded natural killer cells proliferated in vivo in an interleukin-2 dose-dependent fashion, persisted up to 4 weeks, were readily detectable in the human bone, inhibited myeloma growth and protected bone from myeloma-induced osteolysis. (haematologica.org)
  • Drugs used in combination chemotherapy, such as oxaliplatin, leucovorin calcium, and fluorouracil, work in different ways to stop the growth of tumor cells, either by killing the cells, by stopping them from dividing, or by stopping them from spreading. (stanford.edu)
  • Growth inhibition was specifically exerted by DEHP against three leukaemic cell lines at concentrations below 100 microg mL(-1). (tripod.com)
  • At 100 microg mL(-1) DEHP, K562, HL60 and U937 leukaemic cell lines showed growth inhibition of 95, 97 and 95%, respectively. (tripod.com)
  • In a normal cell line, MDBK, DEHP exerted 30% growth inhibition at a concentration of 100 microg mL(-1), and showed no inhibitory activity at concentrations below 50 microg mL(-1). (tripod.com)
  • The growth of human neuroectodermal tumors is inhibited in mice with severe combined immunodeficiency without any appreciable toxic effects on the animals. (tripod.com)
  • The interactions between tumor and immune cells in the local microenvironment are believed to be involved in the growth, metastasis and invasion of GC [ 2 ]. (biomedcentral.com)
  • Seven compounds inhibited the growth of some cancer cell lines, whereas one of the 2-quinoline derivatives displayed favorable activity against all tested cancer cells with GI 50 values of 0.92-13 μM. (springer.com)
  • Cish fl/fl Ncr1 Ki/+ NK cells display lower activation thresholds and Cish fl/fl Ncr1 Ki/+ mice are more resistant to tumor metastasis and to primary breast cancer growth. (bmj.com)
  • Briefly, for testing cell growth in soft agar, 103 cells dissociated from neurospheres were suspended in 3 ml Adv DME containing 5% FBS and 0. (plksignaling.com)
  • uNK cells isolated from obese women had altered expression of genes and pathways associated with extracellular matrix remodeling and growth factor signaling. (jci.org)
  • it has been shown that CD25 serves as a negative growth regulator of Chronic myeloid leukemia leukemic stem cells. (cusabio.com)
  • To verify the cytoplasmic localization of Kaiso in CML BP, we analyzed cytoplasmic Inhibitors,Modulators,Libraries expression of Kaiso protein by western blot examination, evaluating expression in cytoplasmic and nuclear protein extracts in K562 cell line and imatinib resistant K562 cell line. (srcsignaling.com)
  • Important cytoplasmic expression of Kaiso was only observed in K562 cell line whereas in imatinib resistant K562 cell line was clearly down regulated. (srcsignaling.com)
  • We also confirmed the weak expression of Kaiso in imatinib resistant K562 cell line by immunofluorescence. (srcsignaling.com)
  • Currently being a more innovative phase of CML and features a poor prognosis for the patient, given that a few of them are resistant to imatinib therapy, it seemed proper to begin to characterize these cells. (pafr-inhibitor.com)
  • As a result, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 (human wild‑type) or TCCY‑T315I (human imatinib‑resistant) and the Ba/F3‑(T315I/E279K/Y253H) (mouse BCR/ABL point mutation‑transfected cells). (spandidos-publications.com)
  • Interestingly, AVM appeared to be more sensitive to imatinib‑resistant (T315I, Y253H, and E279K) than wild‑type BCR/ABL cells, indicating its potential to overcome imatinib‑resistant severe issues in CML. (spandidos-publications.com)
  • Thus, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 [human wild-type (WT)] or TCCY-T315I [human imatinib-resistant (IR)] and the Ba/F3-(T315I/E279K/Y253H) (mouse BCR/ABL point mutation-tranfected cells). (spandidos-publications.com)
  • QRT PCR evaluation showed that Kaiso mRNA levels had been decreased by 80% and Western blot examination showed that Kaiso protein ranges have been undetectable in K562 cells trans fected by siRNA Kaiso, when when compared to scrambled knock down cells. (srcsignaling.com)
  • Furthermore, activin A reduced the induction effect of these mitogenic cytokines on K562 colony formation in a dose-dependent manner. (tmu.edu.tw)
  • Over lifetime, due to the action of several stressors such as DNA damage and telomere shortening, senescent cells accumulate in the organism and release a variety of pro-inflammatory cytokines responsible for low-grade inflammation. (frontiersin.org)
  • We utilized well defined early response genes (HSP70, Egr-1) and severe stage cytokines (IL-1, IL-6, IL-8, MCP-1), aswell as inflammatory cells, to quantify the lung damage. (cell-signaling-pathways.com)
  • Evidence is particularly strong for a beneficial effect on natural killer (NK) cells [ 2 ], which participate in first line innate immune defense through their cytotoxic activity (NKCA) and release of effector cytokines such as interferon (IFN)γ or tumor necrosis factor (TNF)α [ 3 ]. (springeropen.com)
  • Interestingly, cytokines from type-2 innate immune cells, such as M2 macrophages or D2 dendritic cells exhibit a protective effect on both types of diabetes. (biobran.org)
  • We determined the capacity of the leukemia cell line K562 modified to express a membrane-bound form of interleukin (IL)-15 and 41BB ligand (K562-mb15-41BBL) to generate human NK cells with enhanced cytotoxicity. (aacrjournals.org)
  • Taking advantage of the diverse γδ TCR repertoire or other ligand-receptor interactions, γδ T cells can recognize a broad spectrum of tumor-associated antigens (TAAs) in a major histocompatibility complex (MHC)-independent manner, thereby activating downstream pleiotropic effects. (explorationpub.com)
  • The hormonal activation occurs through specific ligand-receptor interactions on the surface of the target cells. (biomedcentral.com)
  • A different experimental approach by West and Cooke [ 10 ] demonstrated the secretion of M r 80-90K hCG-LH receptor complex into the culture media from ligand-induced rat and mouse leydig cells. (biomedcentral.com)
  • instead, a specific intra-cellular role of truncated receptor in regulating the cell surface expression of a full-length ligand-binding Lhcgr has been proposed [ 15 ]. (biomedcentral.com)
  • Adenine improves the survival of rat Purkinje cells and enhances the storage of erythrocyte in whole blood [ 6 - 8 ]. (hindawi.com)
  • Methods: Human peripheral blood NK cells were isolated by Rosettesep NK sorting kit.The cytotoxic activity of NK cells against human leukemia NB4,K-562,Raji cells and allogeneic mature or dendritic cells (DCs) was detected before or after KIR2DL1 and KIR2DL2/2DL3 were blocked.The effect of NK cells on T lymphocyte proliferation was detected by mixed lymphocyte reaction and TGF-β1 concentration in culture supernatant was measured. (zjujournals.com)
  • DEHP exhibited an inhibitory activity of 74, 83 and 81%, respectively, in K562, HL60 and U937 cell lines at a concentration of 10 microg mL(-1). (tripod.com)
  • At a concentration of 1 microg mL(-1), DEHP exerted an inhibitory activity of 50, 51 and 52%, respectively, in K562, HL60 and U937. (tripod.com)
  • Inhibitory concentration 50. (lookformedical.com)
  • Interestingly, the half maximal inhibitory concentration (IC50) of BLMs in different cancer cell lines varies from nM to μM ranges. (lookformedical.com)
  • All the derivatives were found to less potent against leukemic cancer cell line. (biomedcentral.com)
  • Recently, several studies have revealed that the accumulation of L-kynurenine (L-KYN) mediate immunosuppressive effects in TME [ 10 ]. (biomedcentral.com)
  • Immunofluorescence analysis showed the cytoplasmic distribution accumulation of Kaiso in K562 cell line. (pafr-inhibitor.com)
  • If the greater 17-DMAG-induced cell death in Atm_/_ cells might possibly be attributed to an enhanced accumulation of p53 and Nilotinib the mechanism by way of which this might come about remain unclear. (cd31-signal.com)
  • CISH deletion favors NK cell accumulation to the primary tumor, optimizes NK cell killing properties and decreases TIGIT immune checkpoint receptor expression, limiting NK cell exhaustion. (bmj.com)
  • The compound does not inhibit the proliferation of normal fibroblasts nor that of hemopoietic progenitor cells. (tripod.com)
  • In fact, several studies have shown that T2D induces senescence in multiple types of cells, including fibroblasts and endothelial cells ( 11 , 12 ). (frontiersin.org)
  • Offered that Kaiso is overexpressed while in the cytoplasm of K562 cells, this study set out to examine how reduction of Kaiso and Regorafenib VEGFR their companion p120ctn impacted gene expression and cell proliferation of CML BP. (srcsignaling.com)
  • CYP2C9, CYP2J9, and possibly the more recently characterized CYP2S1 appear to be the main produces of the EETs in humans with CYP2C9 being the main EET producer in vascular endothelial cells and CYP2J9 being highly expressed (although less catalytically active than CYP2C) in heart muscle, kidneys, pancreas, lung, and brain. (wikipedia.org)
  • The inhibitory effect of the extract on human umbilical vein endothelial cells (HUVECs) migration was tested on the wound-healing model with an inverted microscope. (celljournal.org)
  • After human umbilical vein endothelial cells (HUVECs) were suppressed by dose-dependent SO extract, their migrations were detected by wound healing model, yet they were unable to show a dose response effect on proliferation of the different cells (50-200 μg/ml). (celljournal.org)
  • Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2 cells, in a dose-dependent manner. (hindawi.com)
  • Among these derivatives, 8a exhibited more than 10-fold antiproliferation activity in several tumor cells than KX01 and significantly improved in vivo antitumor effects. (mirnainhibitors.com)
  • Yet, for siRNAtreated cells, the interaction lies nearer on the no-interaction line indicating significantly less powerful synergy as also indicated by the interaction parameter worth of 0.413 when compared with 0.243 for your siRNA-control cells. (cd31-signal.com)
  • 1 Significant advances have been made by combining novel agents with autologous peripheral blood stem cell transplantation which allows for long-term disease-free survival in the majority of transplant-eligible patients. (haematologica.org)
  • RNAi knock down of kaiso in K562 cells improves survival and proliferation. (srcsignaling.com)
  • A multivariate Cox analysis was performed to show that the intra-tumoral NK cell density was positively related with the overall and disease-free survival of GC patients [ 9 ]. (biomedcentral.com)
  • Natural killer (NK) cells can kill cancer cells in the absence of prior stimulation and hold considerable potential for cell-based therapies targeting human malignancies ( 1 - 4 ). (aacrjournals.org)
  • In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)
  • These results indicate that DEHP isolated from Aloe vera Linne has a potent antileukaemic effect, and thus represents a new type of pharmacological activity with respect to human leukaemic cells. (tripod.com)
  • Extracts of Aloe vera Linne have been found to exhibit cytotoxicity against human tumour cell lines. (tripod.com)
  • This study examines the anti-tumour effects of di(2-ethylhexyl)phthalate (DEHP) isolated from Aloe vera Linne, in human and animal cell lines. (tripod.com)
  • K562 cells were www.selleckchem.com/products/Bortezomib.html both transfected with siRNA scrambled that will not target any human gene or transfected with siRNA to Kaiso or p120ctn either alone or in combination. (srcsignaling.com)
  • Here we report, using the pathogenic model of antiretroviral therapy-treated, SIV-infected rhesus macaques that sequential interleukin-21 and interferon alpha therapy generate terminally differentiated blood natural killer cells (NKG2a/c low CD16 + ) with potent human leukocyte antigen-E-restricted activity in response to SIV envelope peptides. (nature.com)
  • We constructed a non-contact co-culturing system and humanized xenograft tumor mice model to detect the influence of GC microenvironment on NK-92 or primary human NK cells viability by flow cytometry. (biomedcentral.com)
  • At present, the phenotypic heterogeneity and functional variation of human γδ T cells are increasing. (explorationpub.com)
  • In this review, we use the Heilig nomenclature to describe human γδ T cells [ 1 ]. (explorationpub.com)
  • Finally, we targeted CISH in human NK-92 or primary NK cells, using a technology combining the CRISPR(i)-dCas9 tool with a new lentiviral pseudotype. (bmj.com)
  • We then tested human NK cells functions. (bmj.com)
  • Finally, using CRISPRi, we then targeted CISH in human NK-92 or primary NK cells. (bmj.com)
  • In human NK cells, CISH deletion also favors NCR signaling and antitumor functions. (bmj.com)
  • In this study, we investigated the effects of Accepted 14 September 2009 nine naturally occurring compounds isolated from Citrus jambhiri Lush and Citrus pyriformis Hassk Available online 24 September 2009 (Rutaceae) for their potential to modulate the activity of P-gp in the multidrug-resistant human leukaemiacell line CEM/ADR5000. (gotomydoctor.com)
  • We demonstrate that recombinant LHCGR proteins which include the hormone-binding domain are secreted from transfected cells and that natural LHCGR is also secreted from human placental explants. (biomedcentral.com)
  • The derivatives DS-14 and DS-20 have been found to active against breast cancer cell line and human colon carcinoma cell line having IC 50 in the range of 19-65 µg/ml. (biomedcentral.com)
  • This outcome was confirmed by immunofluorescence in K562 cells transfected by siRNA Kaiso, exhibiting the undetectable ex pression of Kaiso. (srcsignaling.com)
  • Knock down both Kaiso or p120ctn alone or in blend led to sig nificant reduction of Wnt11 when when compared to scrambled knock down cells. (srcsignaling.com)
  • As is recognized that Kaiso interacts with TCF LEF1, and the Wnt11 professional moter, has regulatory web pages for binding TCF protein, these results suggest the inhibitory purpose of TCF LEF1 B catenin within the expression of Wnt11. (srcsignaling.com)
  • In K562 cells trans fected by siRNA p120ctn, Kaiso may well be accountable for Wnt11 repression. (srcsignaling.com)
  • Even though the Kaiso knock down alone didn't show a substantial enhance proliferation, the double knock down showed a significant boost by 51% in proliferation, when compared to scrambled knock down cells. (srcsignaling.com)
  • Consistent with this particular discovering, knock down of both Kaiso or p120ctn alone or in combin ation, in K562 cells, led to a significant 10 100 fold in crease in SCF expression assessed by QRT PCR. (srcsignaling.com)
  • Activated γδ T cells exhibit strong cytotoxic activity and cytokine secretion functions and are effective antitumor lymphocytes with simple and direct recognition modes and rapid responses. (explorationpub.com)
  • Here, we targeted an intracellular inhibiting protein 'cytokine inducible SH2-containing protein' (CISH) in NK cells to evaluate the impact on their functions and antitumor properties. (bmj.com)
  • Conclusion This study represents a crucial step in the mechanistic understanding and safety of Cish targeting to unleash NK cell antitumor function in solid tumors. (bmj.com)
  • The development of immune checkpoint blockers (ICB) aimed at enhancing antitumor T-cell responses has revolutionized cancer treatment. (aacrjournals.org)
  • This effect would be of relevance in the context of cancer given the prominent role of these cells in antitumor immunity. (springeropen.com)
  • An eventual benefit associated with exercise training on NK cells might be of clinical relevance in the context of cancer given the important role of this lymphocyte subpopulation in antitumor immunity [ 14 , 15 ]. (springeropen.com)
  • Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)
  • In line, many MLL-fusions can act as potent oncogenes in cell line models and animal models of leukemia 4 . (nature.com)
  • Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
  • Leukemia cells in patients are intrinsically resistant to standard chemotherapy, and resistance development in leukemia cells toward drugs results in treatment failure[ 4 , 5 ]. (ijpsonline.com)
  • In previous research, the authors demonstrated that the methanol extract of Artemisia vulgaris (AVM) has the ability to inhibit chronic myeloid leukemia (CML) cell proliferation. (spandidos-publications.com)
  • Chronic myeloid leukemia (CML) is a malignancy of the blood and bone marrow that affects children and adults. (spandidos-publications.com)
  • Comparisons of the gene expression profiles of the expanded NK cells and their unstimulated or IL-2-stimulated counterparts showed marked differences. (aacrjournals.org)
  • Global gene expression of Cish fl/fl Ncr1 Ki/+ NK cells compared with Cish +/+ Ncr1 Ki/+ NK cells revealed upregulation of pathways and genes associated with NK cell cycling and activation. (bmj.com)
  • Effects of Trigona honey on the Gene Expression Profile of Pseudomonas aeruginosa ATCC 10145 and Streptococcus pyogenes ATCC 19615. (google.com.my)
  • Results Natural killer cells from healthy donors and myeloma patients expanded a median of 804- and 351-fold, respectively, without significant T-cell expansion. (haematologica.org)
  • The metal complexes of the compounds had stronger cytotoxic effects and cleaved caspase-3 expression than their ligands. (ijpsonline.com)
  • Inspired by the accidental discoveries, we constructed a NK-92 cell strain with high expression of GPX4 and treated the humanized xenograft tumor mice model with the NK-92 cells. (biomedcentral.com)
  • Moreover, we found NK cells with higher GPX4 expression showed resistance to L-KYN induced ferroptosis. (biomedcentral.com)
  • Additionally, the truncated Lhcgr, misrouted the full-length Lhcgr in ER, thereby decreasing the cell surface expression of the mature functional receptor. (biomedcentral.com)
  • This was reflected in higher peripheral percentage of CD4(+) helper T cells and higher expression of FOXP3 and IL-2Ralpha. (cusabio.com)
  • Pixantrone induces DNA damage at high concentrations (500 nM) but not at concentrations (100 nM) sufficient to kill PANC1 cells. (fluoroprobe.com)
  • Briefly, cells seeded into 96-well plates are treated with increasing concentrations of either pixantrone or doxorubicin for 72 hours. (fluoroprobe.com)
  • Anticancer activity of adenine in colon cancer cells is attributable to the activation of apoptotic signaling and in turn the AMPK/mTOR pathway. (hindawi.com)
  • We explored the possible mechanisms underlying the anticancer activity of adenine in colorectal cancer cells. (hindawi.com)
  • Previous studies have shown that activin A inhibited the colony-forming activity of mouse Friend erythroleukemia cells, however, the mechanism remains unknown. (tmu.edu.tw)
  • The frequency and activity of terminally differentiated NKG2a/c low CD16 + natural killer cells correlates with a reduction of replication-competent SIV in lymph node during antiretroviral therapy and time to viral rebound following analytical treatment interruption. (nature.com)
  • Given previous findings on a role of IL-21 and IFNα in regulating NK cell function, and that NKG2a low CD16 + NK cells are generated while expressing high levels of IL-21R in nonpathogenic SIV infection, we sought to determine whether immunotherapy with IL-21 and IFNα rescues AGM-like profiles of NK cell maturation and activity in SIV-infected rhesus macaques (RMs). (nature.com)
  • After activation, they can directly lyse the targets by generating cytotoxic molecules, such as perforin and granzyme B. Besides, NK cells also secret amounts of immune regulators, especially IFN-γ, to enhance the overall anti-tumor activity [ 5 ]. (biomedcentral.com)
  • Inspired by these results, we decided to synthesize KX01 derivatives to extend into the interior of α-tubulin and increase inhibitory activity against tubulin. (mirnainhibitors.com)
  • Treatment of drug-resistant Caco-2 cells with the most active C. jambhiri and C. pyriformis compounds increased their sensitivity to doxorubicin and completely reversed doxorubicin resistance, which agrees with a decreased P- gp activity. (gotomydoctor.com)
  • The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. (cusabio.com)
  • n = 50), which was considerably superior to that produced by stimulation with IL-2, IL-12, IL-15, and/or IL-21 and caused no proliferation of CD3 + lymphocytes. (aacrjournals.org)
  • For example, ex vivo IL-21 treatment expanded CD16 + NK cells 9 , antagonized the IL-15-dependent expansion of resting NK cells 10 , and reverses hypo-responsiveness via the STAT1 and PI3K-AKT-FOXO1 pathways 11 . (nature.com)
  • furthermore, in vivo IFNα-induced NK cell cytotoxicity correlates with reductions in HIV-DNA during antiretroviral therapy (ART) 16 . (nature.com)
  • Pre-incubation from the recombinant sELR1 with EIAV inhibited EIAV an infection in equine macrophages considerably, the principal in vivo focus on cell from the trojan. (angiogenesis-blog.com)
  • Moreover, the complexity of tumor microenvironment (TME), which mainly consists of neoplastic cells, stromal cells, immune cells, soluble factors and extracellular matrix, severely diminishes the efficacy of anti-tumor immunity, thus restraining the effects of various immunotherapies developed during the past decades. (biomedcentral.com)
  • γδ T cells recruited into the tumor microenvironment can act as effector cells to mediate cancer immune surveillance. (explorationpub.com)
  • Therefore, an in-depth understanding of γδ T cells that play conflicting roles in the tumor microenvironment is necessary. (explorationpub.com)
  • This review further emphasizes the diversified cross-talk between γδ T cells and other immune cells. (explorationpub.com)
  • Background The success and limitations of current immunotherapies have pushed research toward the development of alternative approaches and the possibility to manipulate other cytotoxic immune cells such as natural killer (NK) cells. (bmj.com)
  • Together, these findings establish a potentially new link between obesity and poor pregnancy outcomes, and indicate that obesity-driven changes to uterine-resident immune cells critically impair placental development. (jci.org)
  • The data showed that adenine activated AMP-activated protein kinase (AMPK) signaling contributing to autophagic cell death through mTOR in both colon cancer cell lines. (hindawi.com)
  • Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. (cancerindex.org)
  • ABSTRACT: Klisyri (KX01) is a dual tubulin/Src protein inhibitor that has shown potential therapeutic effects in several tumor models. (mirnainhibitors.com)
  • The earliest biochemical evidence on the existence of cell-free soluble LH receptor was the purification of an hCG-binding protein, relative molecular mass of 65K (M r , 65K) from porcine follicular fluid and was based on gel filtration followed by affinity chromatography [ 9 ]. (biomedcentral.com)
  • It was previously shown that Wnt11 can modulate hematopoietic stem cell diversification. (srcsignaling.com)
  • Pixantrone induces cell death in multiple cancer cell lines independent of cell cycle perturbation, with IC 50 s of 37.3 nM, 126 nM and 136 nM for T47D, MCF-10A and OVCAR5 cells, respectively. (fluoroprobe.com)
  • Pixantrone induces cell death through mitotic perturbations and subsequent aberrant cell divisions. (fluoroprobe.com)
  • In the present study, we investigated the anticancer effects of adenine and the underlying mechanism in colon cancer cells. (hindawi.com)
  • Recently, adenine has been explored for its anticancer property in several types of cancer cell lines. (hindawi.com)
  • In this study, we investigated the effects of adenine against the proliferation of colon cancer cells. (hindawi.com)
  • Conclusion: According to our preliminary investigation, Zi Huangjing TM preparation is safe and has the potential therapeutic effect of improving CRF in cancer patients during chemotherapy. (bvsalud.org)
  • Infusions of natural killer (NK) cells are an emerging tool for cancer immunotherapy. (aacrjournals.org)
  • The results that we report here provide rationale and practical platform for clinical testing of expanded and activated NK cells for cell therapy of cancer. (aacrjournals.org)
  • The significant factor contributing to the distant invasion of cancer cells is the ability of tumors to produce large numbers of new blood vessels, known as angiogenesis. (celljournal.org)
  • Her interests also focus on investigating colorectal cancer genomics, and their effect on outcomes and treatment implications. (stanford.edu)
  • Immunotherapy with monoclonal antibodies, such as atezolizumab, may help the body's immune system attack the cancer, and may interfere with the ability of tumor cells to grow and spread. (stanford.edu)
  • PURPOSE: This randomized phase II trial is studying the side effects and how well giving cetuximab and irinotecan hydrochloride with or without ramucirumab work in treating patients with advanced colorectal cancer with progressive disease after treatment with bevacizumab-containing chemotherapy. (stanford.edu)
  • For many cancer patients the side effects of treatments have an adverse impact on their health and immunity, often making it difficult for them to continue with strong medical therapies. (nutraculture.com)
  • Phycocyanin lowers the amount of cyclooxygenase-2 which is up regulated in cancer cells. (nutraculture.com)
  • For example, the PD-1/PD-L1 treatment have shown outstanding effects on several types of cancer, but the objective response rate in GC was only at 15% [ 4 ]. (biomedcentral.com)
  • Using intravenous injection of B16F10 melanoma cell line and EO711 triple negative breast cancer cell line, metastasis evaluation was performed. (bmj.com)
  • T cells play a critical role in the control of cancer. (aacrjournals.org)
  • The derivatives DS-14 and DS-20 have shown potential against Gram-negative bacteria and breast cancer cell line and can be used as a lead for rational drug designing of the antimicrobial and cytotoxic agents. (biomedcentral.com)
  • In this systematic review and meta-analysis, we aimed to summarize current evidence on the effects of exercise training on the levels and function of NK cells in cancer survivors (i.e., from the time of diagnosis until the end of life). (springeropen.com)
  • There was heterogeneity across studies regarding cancer types (breast cancer, non-small cell lung cancer and other solid tumors), treatment (e.g . , receiving vs having received chemotherapy), exercise modes (aerobic or resistance exercise, Tai Chi, Yoga) and duration (2-24 weeks). (springeropen.com)
  • Controversy exists, however, on the actual effects of exercise training on NK cells in patients with cancer. (springeropen.com)
  • Although there is biological rationale for a potential benefit of exercise training on NK cells, current evidence does not support a significant effect in cancer survivors. (springeropen.com)
  • The figures indicate that for each the siRNA-treated and -control cells, the interaction line lies beneath the no-interaction line indicating mechanism-based tsa inhibitor synergy. (cd31-signal.com)
  • The time point at which treatment with the inhibitor was most potent, was found to coincide with the expected moment of entry of the virion with the host cell. (biomedcentral.com)
  • TREGs suppress the activation and expansion of autoreactive T-cells. (cusabio.com)
  • The values of LD50 (lethal dose 50%) calculated from experiments are different for each cell line . (lookformedical.com)
  • So we started off by characterizing its subcellular distribution in K562 cell line since it has been regarded as as a cellular model of CML BP. (pafr-inhibitor.com)
  • We confirmed that AEBSF inhibition is independent of the cell line used or RSV strain. (biomedcentral.com)
  • Results are in line with the hypothesis that in the early phase of ALS, neuroprotective helper T cells infiltrate in the affected areas in the lumbar spinal cord. (cusabio.com)
  • The anti-angiogenic effect of the SO extract was evaluated on chicken chorioallantoic membrane (CAM) neovascularisation model, microscopically. (celljournal.org)
  • Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)
  • However, the applicability of such traditional chemotherapeutic approaches is primarily limited due to acquired Multi-Drug Resistance (MDR) in cells, which is acquired through the production of anti-apoptotic cascades and the overexpression of cell membrane-based drug efflux pumps, such as P-glycoprotein, for self-defense. (ijpsonline.com)
  • However, it is unclear whether these molecular mechanisms pertain to the entire family of MLL-fusions or if they specifically affect the leukemogenicity of isolated MLL-fusion proteins. (nature.com)
  • First, γδ T cells exposed to complicated cytokine networks are potentially affected by multiple inhibitory mechanisms. (explorationpub.com)
  • Adverse and beneficial effects of plant extracts on skin and skin disorders. (tripod.com)
  • The inhibition of p38 MAPK reverted the inhibitory effect of activin A on mitogenic cytokine-mediated K562 colony formation. (tmu.edu.tw)
  • Pixantrone also shows preventive and therapeutic effect in experimental autoimmune myasthenia gravis (EAMG) rats [4] . (fluoroprobe.com)
  • Based on this, we generated GPX4 over-expressed NK-92 cells, and found these cells showed therapeutic potential towards GC. (biomedcentral.com)
  • Herein, ethanol extract of Salvia officinalis (SO) has been analyzed for its anti-angiogenic, anti-proliferation and anti-migration activities. (celljournal.org)
  • Results In Cish fl/fl Ncr1 Ki/+ mice, we detected no developmental or homeostatic difference in NK cells. (bmj.com)
  • Methodological quality of the studies was assessed with the PEDro scale, and results were meta-analyzed using a random effects (Dersimoian and Laird) model. (springeropen.com)
  • We found L-KYN, mainly generated through indoleamine 2, 3-dioxygenase (IDO) from GC cells, impaired NK cells viability in TME. (biomedcentral.com)
  • In previous research, the authors demonstrated that the methanol extract of A. vulgaris (AVM) has the ability to inhibit the viability of CML cells ( 10 ). (spandidos-publications.com)
  • To the best of our knowledge, this is the first comprehensive review on natural actinobacterial compounds affecting different types of CRC. (frontiersin.org)
  • Compounds (C1-8 and doxorubicin) were placed to 96-well culture plate in triplicate order at doses of 1, 10, 100, 1000 μM and then K562 cells were seeded into the wells at the dose of 105/ml. (ijpsonline.com)
  • Moreover, the effects of various sub‑fractions of AVM were then investigated in order to determine the optimal solvent for the identification of anticancer bioactive compounds. (spandidos-publications.com)