• Light effects on the expression of nuclear genes for plastid proteins and for the 18S, 5.8S and 25S ribosomal RNAs are discussed, together with some recent information concerning the expression of chloroplast genes in developing plastids. (ncsu.edu)
  • This mechanism is governed by several cell signalling pathways, one of which involves a nuclear export protein (allowing proteins and RNA to pass between the nucleus and the cytoplasm), XPO1. (longlonglife.org)
  • The study also highlights the therapeutic potential of targeting nuclear export proteins and transcription factors in humans to combat diseases associated with proteostasis decline, including age-related neurodegenerative diseases. (longlonglife.org)
  • BET bromodomain proteins function as master transcription elongation factors independent of CDK9 recruitment. (rndsystems.com)
  • They exert their biological functions by competitively binding to microRNAs (miRNAs) as miRNA sponges, promoting gene transcription and participating in the regulation of selective splicing, interacting with proteins and being translated into proteins. (biomedcentral.com)
  • They were nanoscale membrane vesicles that were generated by most types of cells, which can convey information in the tumor microenvironment by transferring cargos such as DNA, RNA, proteins and lipids, therefore they are critical to tumor progression [ 5 ]. (biomedcentral.com)
  • This review focuses on an up-to-date understanding of the functions of E3 ligases in cancers and debates the perspectives of cancer cells that rely on inhibition or activation of ubiquitylation of target proteins. (bmbreports.org)
  • The irradiation resulted in lower levels of mtDNA (i.e. lower mtDNA copy number per cell) lower levels of ATP lower levels of oxygen consumption and dose-responsive inhibition of larval development (39 40 The mRNA levels for mtDNA-encoded proteins were initially lower than in unexposed nematodes but later rebounded (40). (biodiversityhotspot.org)
  • NF90ctelevision can be a C-terminal variant [30] from the NF90 double-stranded RNA (dsRNA)-binding proteins that was originally reported like a putative transcription element knowing the antigen receptor response component (ARE) in the IL-2 gene regulatory area [31]. (thetechnoant.info)
  • This process is carried out by the human spliceosome machinery, in which over 300 proteins sequentially assemble with uridine-rich small nuclear RNA molecules (U snRNAs) to form distinct small nuclear ribonucleoprotein complexes (snRNPs). (biomedcentral.com)
  • Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)
  • Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)
  • RNAs, including long noncoding RNAs (lncRNA), are known to be abundant and important structural components of the nuclear infrastructure. (harvard.edu)
  • Several studies have clearly demonstrated that distinct cytokine activated signalling and transcription factors regulate the commitment of a naïve T cell along the Th1 or T helper cell type 2 (Th2) phenotype, as well as maintenance of the polarised phenotype. (bmj.com)
  • For example, Cirs-7 is derived from a highly conserved single exon, also known as antisense transcription of cerebellar degeneration-related protein 1 (CDR1as), which can regulate miR-7 through adsorption, thus reducing the inhibition of miR-7 on its target [ 19 ]. (biomedcentral.com)
  • Splicing factor such as Serine Arginine rich splicing factor (SRSF1) [ 16 ] regulate HIV-1 transcription whereas, Small nuclear RNAs [ 17 ] inhibit HIV-1 replication through excessive RNA Splicing. (biomedcentral.com)
  • PARP-1 recognizes DNAstrand interruptions and can complex with RNA and negatively regulate transcription. (ipa2014.org)
  • Noncoding RNA sequences can regulate gene expression via interactions with epigenetic and other control mechanisms. (stanford.edu)
  • MicroRNAs (miRNAs) and other classes of short non-coding RNAs regulate essential processes in the development and function of the nervous system. (harvard.edu)
  • MicroRNAs (miRNAs) are small endogenous RNAs that pair with sequences in messenger RNAs to direct post-transcriptional repression. (wikipedia.org)
  • We propose that Set1 modulates adaptive responses, as exemplified by the post-transcriptional inhibition of Ty1 retrotransposition. (prinsesmaximacentrum.nl)
  • Hastings, M. L., Ingle, H. A., Lazar, M. A. & Munroe, S. H. Post-transcriptional regulation of thyroid hormone receptor expression by cis-acting sequences and a naturally occurring antisense RNA. (nature.com)
  • Different mRNAs are characterized by different nuclear-cytoplasmic `partitioning coefficients', indicating that post-transcriptional events play a significant role in regulating the accumulation of these mRNAs during light induction. (ncsu.edu)
  • Altered microRNA expression occurs through hyper/hypo-methylation of CpG sites in CpG islands in promoters controlling transcription of the microRNAs. (wikipedia.org)
  • Silencing of transcription of a gene may be initiated by other mechanisms, but this is often followed by methylation of CpG sites in the promoter CpG island to cause the stable silencing of the gene. (wikipedia.org)
  • It is concluded that cytosine methylation in rDNA is regulated and that the methylation pattern correlates with the transcription potential of an rRNA gene. (ncsu.edu)
  • One family of molecules are the small GTPases, such as Rho GTPases, Ran GTPases, and Rab GTPases and another are the actin nucleators, such as the formin family and the Arp2/3 complex. (bioone.org)
  • Their regulation may be carried out either through direct binding to DNA as peroxisome proliferator-activated receptors or via modulation in an indirect manner of signaling pathway molecules (e.g., protein kinase C) and other transcription factors (nuclear factor kappa B and sterol regulatory element binding protein). (springer.com)
  • In addition, we uncovered that Set1 binds to different classes of RNAs to levels that largely exceed the levels of binding to the general population of transcripts, suggesting the Set1 persists on these RNAs after transcription. (prinsesmaximacentrum.nl)
  • This generates a vast repertoire of transcripts that includes protein-coding messenger RNAs, long non-coding RNAs (lncRNAs) and repetitive sequences, such as SINEs (short interspersed nuclear elements). (nature.com)
  • As expected, IFN-γ but not IL-4 RNA transcripts were increased in the mucosa of CD patients in comparison with controls. (bmj.com)
  • For many regions of the genome, we have detected multiple overlapping transcripts including both small, gene-sized RNAs and large transcripts covering entire gene clusters. (ncsu.edu)
  • All transcripts detected were more abundant (as a fraction of total cellular RNA) in light grown plants than in plants entirely in the dark. (ncsu.edu)
  • Nuclear-Cytoplasmic Partitioning of Phytochrome-Regulated Transcripts in Pisum sativum. (ncsu.edu)
  • Transcripts from each of five light-regulated genes exhibited different responses to a variety of light treatments, but for each transcript we observed a characteristic linear relationship between nuclear and cytoplasmic levels over a wide range of total transcript abundance. (ncsu.edu)
  • The nascent transcripts from HIV-1 Longer Terminal Do it again (LTR) include a exclusive structured RNA domains inside the 5′-nontranslated area referred to as the transactivation response (TAR) component which is crucial for effective transcription of viral promoter in response to Tat [3,4]. (thetechnoant.info)
  • Sequential assembly of the human spliceosome on RNA transcripts regulates splicing across the human transcriptome. (biomedcentral.com)
  • We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)
  • Inhibition of this protein sequesters HLH-30, a transcription factor that regulates autophagy, within the nucleus and promotes its action on DNA[2]. (longlonglife.org)
  • Nuclear RNA binding regulates TDP-43 nuclear localization and passive nuclear export. (rndsystems.com)
  • Deletion of AMPKα1 or AMPKα2 resulted in activation of STAT1 and in increases in proinflammatory mediators, both of which were attenuated by administration of STAT1 small interfering RNA or fludarabine, a selective STAT1 inhibitor. (diabetesjournals.org)
  • Pharmacological perturbation of CDK9 using selective CDK9 inhibition or degradation. (rndsystems.com)
  • Antisense Uchl1 function is under the control of stress signalling pathways, as mTORC1 inhibition by rapamycin causes an increase in UCHL1 protein that is associated to the shuttling of antisense Uchl1 RNA from the nucleus to the cytoplasm. (nature.com)
  • Antisense Uchl1 RNA is then required for the association of the overlapping sense protein-coding mRNA to active polysomes for translation. (nature.com)
  • Mechanistically, we found that AMPK activation increased, whereas AMPK inhibition decreased, the levels of mitogen-activated protein kinase phosphatase-1 (MKP-1), an inducible nuclear phosphatase, by regulating proteasome-dependent degradation of MKP-1. (diabetesjournals.org)
  • Our findings revise theories of labour division between the major RNA polymerases, and identify nucleolar Pol II as a major factor in protein synthesis and nuclear organization, with potential implications for health and disease. (nature.com)
  • mRNA steady state levels of the chlorophyll a/b-binding protein of photosystem II, ferredoxin I, the small and large subunits of ribulose 1,5-bisphosphate carboxylase, and pEA214 and pEA207, two other light-responsive genes, were determined during chlorophyll photooxidation. (ncsu.edu)
  • Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. (cancerindex.org)
  • C. elegans' nuclear export protein XPO1 is the orthologue (the same protein for the same function in different species) of a protein preserved in humans, Exportin-1, and HLH-30 is a functional orthologue protein of TFEB[2]. (longlonglife.org)
  • Inhibiting the nuclear export protein XPO-1 in humans could be applied in therapy, against diseases characterized by lysosomal abnormalities and age-related autophagy abnormalities. (longlonglife.org)
  • Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of Transcription. (rndsystems.com)
  • Rabbit polyclonal to Parp.Poly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-bindingzinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure,and apoptosis. (ipa2014.org)
  • FASN inhibition suppressed E2-stimulated breast cancer cell proliferation and anchorageindependent colony formation while promoting the reduction of ERα protein. (elsevierpure.com)
  • Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)
  • CircRNAs, a new type of endogenous noncoding RNA (ncRNA), are described as covalent closed-loop structure without 5' caps and 3' poly (A) tails. (biomedcentral.com)
  • In addition, several potential functions of circRNAs have been reported: (1) CircRNAs competitively bind to miRNAs as endogenous competitive RNAs (ceRNAs), known as miRNA sponges (Fig. 1 a). (biomedcentral.com)
  • Although the current generation of dCas9-based transcription activators is able to achieve up-regulation of some endogenous loci, the magnitude of transcriptional up-regulation achieved by individual single-guide RNAs (sgRNAs) 12 typically ranges from low to ineffective 8 - 11 . (cdc.gov)
  • For more than 20 years, microRNAs have been known to act in the cytoplasm to degrade transcriptional expression of specific target gene messenger RNAs (see microRNA history). (wikipedia.org)
  • Nuclear and cytoplasmic mRNAs for several phytochrome-regulated genes were examined in Pisum seedlings in order to investigate possible light effects on mRNA partitioning between the nucleus and cytoplasm. (ncsu.edu)
  • Sirtuins or "silent information regulators" of gene transcription, are a family of enzymes which are expressed throughout all phyla of life. (frontiersin.org)
  • This class of noncoding RNAs is small, single stranded, and 19-25 nucleotide long that act as negative regulators involved in posttranscriptional silencing of the gene expression [ 5 ]. (hindawi.com)
  • In addition to classical gene regulatory networks noted above, we have recently identified a central role for additional biologic mechanisms, namely gene regulation by chromatin regulators and by noncoding RNAs. (stanford.edu)
  • The function of histone modifying epigenetic regulators and noncoding RNA as central mediators of epithelial stem cell renewal and differentiation represent major emerging areas of study in the lab. (stanford.edu)
  • The metabolic abnormalities of type 2 diabetes, including hyperglycemia, dyslipidemia, and insulin resistance, activate the Janus kinases/signal transducer and activator of transcription (JAK/STAT) signaling pathway, a major intracellular inflammatory cascade that transmits the intracellular signaling to the nucleus ( 2 ), promoting inflammatory response, inducing insulin resistance ( 3 ), and accelerating the development of cardiovascular complications ( 4 ). (diabetesjournals.org)
  • In this study, switching off the gene coding for XPO1 (xpo-1) led to improved autophagy and enrichment of the transcription factor HLH-30 in the nucleus. (longlonglife.org)
  • It would seem that the effects resulting from the inhibition of XPO1 are also preserved in humans, since analyses performed on HeLa cells showed that the concentration of TFEB in the nucleus as well as autophagy were increased following this inhibition[1]. (longlonglife.org)
  • It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions. (cancerindex.org)
  • The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. (cancerindex.org)
  • In cancers, loss of expression of genes occurs about 10 times more frequently by transcription silencing (caused by promoter hypermethylation of CpG islands) than by mutations. (wikipedia.org)
  • Subsequently, truncation and mutation of a vimentin promoter demonstrated that HDAC1‑induced vimentin expression was dependent on a nuclear factor κ‑light‑chain‑enhancer of activated B cells (NF‑κB) binding site in the vimentin promoter sequence. (spandidos-publications.com)
  • The bacterial genes encoding chloramphenicol acetyltransferase (CAT) and beta-glucuronidase (GUS) were transiently expressed in leaf discs from the AR1 promoter in TGMV A. The levels of AR1 and GUS RNAs were similar in leaf discs after adjusting for viral DNA copy number, while CAT RNA was less abundant. (ncsu.edu)
  • The type I interferon (IFN/) bind to the IFN promoter, and stimulate transcription. (cdc.gov)
  • The principal part of Tat may actually be to market set up of pre-initiation complicated, thereby advertising both transcription initiation and elongation of HIV-1 promoter [4]. (thetechnoant.info)
  • Synthesis of AR1 RNA was dependent on T-DNA transfer and TGMV DNA replication, demonstrating that it is a plant transcription product. (ncsu.edu)
  • Molecular subtypes of small cell lung cancer: a synthesis of human and mouse model data. (cancerindex.org)
  • During mitosis, cells undergo symmetrical cell division, while oocyte meiotic maturation undergoes two consecutive, asymmetric divisions that generate a totipotent haploid oocyte and two small polar bodies not involved in DNA replication. (bioone.org)
  • Background Examination of sponsor cell-based inhibitors of HIV-1 transcription could be very important to attenuating viral replication. (thetechnoant.info)
  • We analyzed the TAR RNA binding properties of NF90ctelevision and show it attenuates HIV-1 replication partly by inhibition of Tat-mediated transactivation of HIV-1 MYL2 LTR. (thetechnoant.info)
  • This suggests that DNA replication following escape from G1 arrest in drug is more error prone and provides a potential explanation for the DNA damage observed under long-term RAF-MEK-ERK1/2 pathway inhibition. (babraham.ac.uk)
  • CpG islands also occur frequently in promoters for functional noncoding RNAs such as microRNAs. (wikipedia.org)
  • Thus microRNAs with hypermethylated promoters may be allowing enhanced transcription of hundreds to thousands of genes in a cancer. (wikipedia.org)
  • Some nuclear microRNAs have been shown to mediate transcriptional gene activation or transcriptional gene inhibition. (wikipedia.org)
  • Tiny interference RNA technologies was employed to cut back Livin gene expression. (liverxreceptor.com)
  • To date, various genome-scale loss-of-function screening methods have been developed, including approaches employing RNA interference 1 , 2 and the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindromic repeat) adaptive immune system 3 , 4 . (cdc.gov)
  • Chromatin structure and expression of plant ribosomal RNA genes. (ncsu.edu)
  • DNase I sensitivity of ribosomal RNA Genes in chromatin and nucleolar dominance in wheat. (ncsu.edu)
  • Chances are consequently, that Tat facilitates chromatin adjustments, thereby advertising initiation and transcription elongation in some sequential, coordinated occasions that result in high degrees of HIV transcription [11]. (thetechnoant.info)
  • In keeping with this look at, we mentioned that Tat/TAR-specified CDK9 (P-TEFb) kinase activity is crucial for the phosphorylation of RNAP II, transcription elongation elements SPT5 and Tat-SF1 as well as the induction histone H3 lysine 4 and lysine 36 methylations during transcriptional activation of integrated HIV-1 chromatin [12]. (thetechnoant.info)
  • Here we show that the Set1 complex (Set1C) directly binds RNA in vitro through the regions that comprise the double RNA recognition motifs (dRRM) and N-SET domain within Set1 and its subunit Spp1. (prinsesmaximacentrum.nl)
  • NF90 offers two dsRNA binding motifs, a putative nuclear localization sign (NLS), and a leucine-rich nuclear export sign (NES). (thetechnoant.info)
  • The C-terminal part of NF90 includes an arginine, glycine-rich (RGG) domains, like the motifs which mediate RNA binding by hnRNP-U and nucleolin [34]. (thetechnoant.info)
  • Existing models suggest that RNA polymerases I and III (Pol I and Pol III) are the only enzymes that directly mediate the expression of the ribosomal RNA (rRNA) components of ribosomes. (nature.com)
  • Traditionally, the nucleolar Pol I and nucleoplasmic Pol III are viewed as the sole mammalian RNA polymerases that directly mediate housekeeping ribosome biogenesis. (nature.com)
  • PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element. (nature.com)
  • Here we show, however, that RNA polymerase II (Pol II) inside human nucleoli operates near genes encoding rRNAs to drive their expression. (nature.com)
  • The shield prevents Pol I from producing sense intergenic noncoding RNAs (sincRNAs) that can disrupt nucleolar organization and rRNA expression. (nature.com)
  • To analyse expression of transcription factors which control the Th1 cell commitment in CD. (bmj.com)
  • Interferon γ (IFN-γ) and interleukin (IL)-4 RNA expression was examined in T lamina propria lymphocytes by quantitative reverse transcription-polymerase chain reaction. (bmj.com)
  • Transient expression of heterologous RNAs using tomato golden mosaic virus. (ncsu.edu)
  • The geminivirus transient expression system allows rapid analysis of RNAs transcribed from foreign genes and can serve as a preliminary screen in the construction of transgenic plants. (ncsu.edu)
  • The results illustrate a complex set of interactions regulating expression of the nuclear and chloroplast genomes. (ncsu.edu)
  • Although there are seven mammalian sirtuins (SIRT1-7), little is known about their expression in the aging brain. (frontiersin.org)
  • ETS family members transcription factors control the appearance of oncogenes, tumor suppressor genes, and various other genes linked to vessel development, invasion, and metastasis, and expression of the elements correlates with poor survival [7C10] often. (columbiagypsy.net)
  • FASN blockade resulted in the increased expression and nuclear accumulation of the cyclin-dependent kinase inhibitors p21WAF1/CIP1 and p27Kip1, two critical mediators of the therapeutic effects of antiestrogen in breast cancer, while inactivating AKT, a key mediator of E2-promoted anchorage-independent growth. (elsevierpure.com)
  • Targeted expression of a neurogenic transcription factor, NeuroD1, in microglia/macrophages in the injured striatum enables their conversion into induced neuronal cells that functionally integrate into the existing neuronal circuits. (bvsalud.org)
  • Competition for PRPF8 availability alters the transcription-coupled splicing of RNAs in which weak 5′ splice sites predominate, enabling diversification of human gene expression during biological processes like mitosis. (biomedcentral.com)
  • to other RNAs may initiate epige- cleus pathways. (who.int)
  • Actin filaments widely involve into multiple cellular processes such as nuclear positioning, germinal vesicle breakdown, spindle migration, chromosome segregation, spindle rotation and polar body extrusion in oocyte mammalian meiosis. (bioone.org)
  • Mammalian mitochondria contain multiple small genomes. (biodiversityhotspot.org)
  • This may include oncomir (oncogenic miRNA) inhibition, or tumor suppressor-miRNA replacement therapies [ 6 , 9 ]. (hindawi.com)
  • Here we identify a nuclear-enriched lncRNA antisense to mouse ubiquitin carboxy-terminal hydrolase L1 ( Uchl1 ), a gene involved in brain function and neurodegenerative diseases 11 . (nature.com)
  • The ratio between AR1 RNA and the major leftward RNA was constant and was used to normalize AR1 transcription for viral DNA copy number. (ncsu.edu)
  • The HIV-1 life cycle includes two essential processes, reverse transcription, forming the linear double stranded DNA (cDNA) and the integration of viral dsDNA into host genome. (biomedcentral.com)
  • In most cases, circRNAs are produced by back splicing of precursor messenger RNA (pre-mRNA), in which the downstream 5'-splicing donor is connected to the upstream 3'-splicing receptor by a 3'-5'-phosphodiester bond [ 11 ]. (biomedcentral.com)
  • Ribosomal RNA genes at different nucleolar organizer (NOR) loci in hexaploid wheat are expressed at different levels. (ncsu.edu)
  • This study indicates that chloroplast genes are often grouped into multigene transcriptional units which can be cotranscribed, and that light-stimulated plastid development involves changes in the relative abundance of the overlapping RNAs of different length that result from transcription of these genes or gene clusters. (ncsu.edu)
  • We reveal a nucleolar Pol-II-dependent mechanism that drives ribosome biogenesis, identify disease-associated disruption of nucleoli by noncoding RNAs, and establish locus-targeted R-loop modulation. (nature.com)
  • ERKi treatment of cells drives the poly-ubiquitylation and proteasome-dependent turnover of ERK2 and pharmacological or genetic inhibition of Cullin-RING E3 ligases prevents this. (babraham.ac.uk)
  • The inhibitors of Tat/TAR axis consist of TAR RNA decoys [15,16], little molecule inhibitors and ribozyme [17-24]. (thetechnoant.info)
  • Innate or acquired resistance to small molecule BRAF or MEK1/2 inhibitors (BRAFi or MEKi) typically arises through mechanisms that sustain or reinstate ERK1/2 activation. (babraham.ac.uk)
  • We are currently pursuing studies of the dominant signaling and gene regulatory networks that control this process, including the Ras/MAPK cascade, which is required for stem cell-mediated self-renewal and the p53 transcription factor family member, p63, which is required for epidermal differentiation. (stanford.edu)
  • Medulloblastoma is defined by the World Health Organization (WHO) as "an embryonal neuroepithelial tumor arising in the cerebellum or dorsal brainstem, presenting mainly in childhood and consisting of densely packed small round undifferentiated cells with mild to moderate nuclear pleomorphism and high mitotic count. (medscape.com)
  • Earlier KLHL1 antibody studies have shown that knocking-down Fli-1 prospects to designated growth inhibition and death in erythroleukemic cells, indicating a possible use of Fli-1 like a restorative target to induce tumor suppression [18C20]. (columbiagypsy.net)
  • Ubiquitylation of MYC couples transcription elongation with double-strand break repair at active promoters. (rndsystems.com)
  • Continuous trypsin inhibition or protease-activated receptor 2 (PAR2) antagonism in the tongue significantly inhibited SCC-induced mechanical sensitization. (bvsalud.org)
  • Hypomethylation of CpG islands in promoters results in increased transcription of the genes or gene sets affected. (wikipedia.org)
  • This transcription factor, by its role on autophagy, plays a role in extending the life span of many model organisms, including C. elegans, by triggering an appropriate response during stress (fasting, heat stress, bacterial infection…)[1]. (longlonglife.org)
  • Changes in dietary fatty acids, specifically the polyunsaturated fatty acids of the ω-3 and ω-6 families and some derived eicosanoids from lipoxygenases, cyclooxygenases, and cytochrome P-450, seem to control the activity of transcription factor families involved in cancer cell proliferation or cell death. (springer.com)
  • As the material increased in purity, there are more high-aspect-ratio particulates and a corresponding distinct increase in cytotoxicity, nuclear factor-B transcription, and inflammasome activation. (cdc.gov)
  • Nuclear positioning, germinal vesicle breakdown, spindle migration, spindle rotation, chromosome segregation, and polar body extrusion are the most critical cellular processes during oocyte meiosis I and II, and a growing number of studies primarily using the mouse oocyte model revealed that actin filaments were critical for these processes, especially for spindle migration. (bioone.org)
  • Here we show that eight different ERKi (both catERKi or dmERKi) drive the turnover of ERK2, the most abundant ERK isoform, with little or no effect on ERK1. (babraham.ac.uk)
  • Similar analyses were performed for RNAs extracted from pea seedlings grown under several different light regimes. (ncsu.edu)
  • Aerobic, inactive forms of Azotobacter vinelandii hydrogenase: activation kinetics and insensitivity to C2H2 inhibition. (ncsu.edu)
  • Experimental enhancement of splice-site strength in mini-gene constructs overcomes the effects of PRPF8 depletion on the kinetics and fidelity of splicing during transcription. (biomedcentral.com)
  • More recently, as an affiliated member of the Institute for RNA , he has been developing emerging methods in the JFBSB to serve the RNA community at Penn, including the application of multiwavelength analytical ultracentrifugation, SEC-MALS, and synchrotron SEC-SAXS to the characterization of mRNA lipid nanoparticles in collaboration with the Mitchell group at Penn. (upenn.edu)
  • In cultured human aortic smooth muscle cells, pharmacologic or genetic activation of AMPK inhibited the signal transducer and activator of transcription-1 (STAT1), while inhibition of AMPK had opposite effects. (diabetesjournals.org)
  • RNA binding is important to define the appropriate topology of Set1C distribution along transcription units and correlates with the efficient deposition of the H3K4me3 mark. (prinsesmaximacentrum.nl)
  • The small compound C75, a synthetic slow-binding inhibitor of FASN activity, induced a dramatic augmentation of estradiol (E2)-stimulated, ERα-driven transcription. (elsevierpure.com)