• TDP-43 is a highly conserved, ubiquitously expressed, multifunctional nucleic acid-binding protein composed of two RNA recognition motifs (RRM), nuclear localization (NLS) and export signals (NES), and a carboxy-terminal glycine rich region. (nature.com)
  • 2015) The SRSF1 RNA Recognition Motifs of are strong inhibitors of HIV-1 replication. (fau.edu)
  • The latter chapters contain NMR chemical shift perturbation experiments that identified the RNA Recognition Motifs (RRM) 2 and 3 of PABP1 as the main contact interface with the Pdcd4 protein. (le.ac.uk)
  • Functionally, CRISPR/Cas9-mediated single amino acid mutagenesis of the head domain blunted MYC expression and MEK phosphorylation, and abrogated triple-negative breast cancer in vivo tumour growth. (tuni.fi)
  • Integrin-FAK signaling has been shown to activate a number of biological mechanisms through phosphorylation and protein-protein interactions promoting tumorigenesis. (aacrjournals.org)
  • The best characterized FAK phosporylation event is the auto-phosphorylation at the tyrosine397 (Tyr397) which creates a motif that is recognized by various SH2 domain-containing proteins, such as src which phosphorylates FAK on Tyr576. (aacrjournals.org)
  • The c-terminal domain of FAK undergoes to several serine-phosphorylation events whose role is not well-understood. (aacrjournals.org)
  • Both vinY822F and vin −/− cells exhibit increased interaction between paxillin and focal adhesion kinase (FAK) and increased paxillin and FAK phosphorylation. (rupress.org)
  • Activation of AMPK by low energy supply and phosphorylation of LKB1 results in an inhibition of mTOR , thus decreasing energy-consuming processes, in particular translation and, thus, cell growth. (sdbonline.org)
  • In their work, the Unit of Structural Dynamics of Macromolecules presented the structure of the DNA-bound PolD-PCNA complex from P. abyssi at 3.77 Å using an integrative structural biology approach, combining cryo-EM, X-ray crystallography, protein-protein interaction measurements and activity assays. (pasteur.fr)
  • Furthermore, structural comparisons with other bacterial Fic proteins, such as Fic of Neisseria meningitidis and of Shewanella oneidensis, show that α(inh) frequently constitutes an amino-terminal or carboxy-terminal extension to the FIC domain, respectively, partially obstructing the ATP binding site in an intramolecular manner. (rcsb.org)
  • Structural homology modelling of all annotated Fic proteins indicates that inhibition by α(inh) is universal and conserved through evolution, as the inhibitory motif is present in ∼90% of all putatively adenylylation-active FIC domains, including examples from all domains of life and from viruses. (rcsb.org)
  • Here, we report molecular level details and structural mechanisms of PP2A-B56α inhibition by an oncoprotein CIP2A. (tuni.fi)
  • Structural modeling and protein docking analyses were performed for ORF7a. (news-medical.net)
  • The structural biology laboratory utilises a variety of techniques including Protein Crystallography, Cryo-electron Microscopy and associated techniques such as Isothermal Titration Calorimetry, which are then combined with functional studies or serve as a basis for structure-based drug design. (nottingham.ac.uk)
  • Structural basis of the leukocyte integrin Mac-1 I-domain interactions with the platelet glycoprotein Ib. (nottingham.ac.uk)
  • In 2015, she joined the Laboratory of Structural Biology Research and the Protein Expression Laboratory at the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS). (nih.gov)
  • Dr. Eren's research focuses on understanding the structural and mechanistic aspects of Rev-host protein interactions and finding target regions to inhibit Rev activity using antibodies, synthetic peptides, and aptamers. (nih.gov)
  • Ig-Superfamily CADMs are named after the presence of one or more Immunoglobulin-like domains in their extracellular region, which are characteristic structural motifs found in antibodies. (pharmiweb.com)
  • The translated polyprotein consists of three structural [capsid (C), membrane (M) and envelope (E)] and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5). (spandidos-publications.com)
  • The multifunctional regulator nuclear factor erythroid 2-related factor (Nrf2) is considered not only as a cytoprotective factor regulating the expression of genes coding for anti-oxidant, anti-inflammatory and detoxifying proteins, but it is also a powerful modulator of species longevity. (springer.com)
  • The major characteristics of Nrf2 are to some extent mimicked by Nrf2-dependent genes and their proteins including heme oxygenase-1 (HO-1), which besides removing toxic heme, produces biliverdin, iron ions and carbon monoxide. (springer.com)
  • This important work substantially advances our understanding of the interplay between genetic variation, chromatin, and genes within topologically associated domains and how this interplay regulates gene expression. (elifesciences.org)
  • His research expanded to the mechanism regulating the transcription of cellular and viral genes and characterized the protein SRSF1 as a strong inhibitor of HIV-1 replication. (fau.edu)
  • Forkhead box protein M1 (FOXM1) is a key transcription factor (TF) that regulates a common set of genes related to the cell cycle in various cell types. (mdpi.com)
  • After irradiation, the expression of DDR-related genes and proteins in ΔNp63α-expressing and control cells was analysed by RT-qPCR, Western blotting, and flow cytometry. (researchsquare.com)
  • Although AmlR apparently is a local transcriptional regulator of the aml operon, the Δ amlR strain shows severe growth inhibitions on glucose and - concomitantly - differential transcription of several genes of various functional classes. (frontiersin.org)
  • Tumor suppressor genes code for proteins that inhibit cell division and growth, acting as a control mechanism to prevent excessive cell proliferation. (pharmiweb.com)
  • At the cellular level, the actions of glucocorticoids are mediated by a 94-kd protein, the glucocorticoid receptor (GR). The human (h) GR belongs to the steroid/thyroid/retinoic acid superfamily of nuclear receptors and functions as a ligand-dependent transcription factor that regulates the expression of glucocorticoid-responsive genes positively or negatively. (medscape.com)
  • Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
  • Subsequently, PIAS3 was also found to be a regulator protein of other key transcription factors, including MITF, NFκB, SMAD and estrogen receptor. (wikipedia.org)
  • ii) Determination of the platelet receptor glycoprotein Ib extracellular domain and von Willebrand factor A1 domain structures (iii) determination of the coagulation factor xi structure. (nottingham.ac.uk)
  • Entry of HIV-1 into human lymphoid requires activities of viral envelope glycoproteins gp120 and gp41, and two host-cell proteins, the primary receptor CD4 and a chemokine co-receptor. (biomedcentral.com)
  • In contrast, the intestinal SALSA was more enriched with the scavenger receptor cysteine-rich domains. (researchgate.net)
  • BMP receptor blockade overcomes extrinsic inhibition of remyelination and restores neurovascular homeostasis. (neurotree.org)
  • Human cytomegalovirus G protein-coupled receptor US28 promotes latency by attenuating c-fos. (uc.edu)
  • Eph and ephrin proteins interact with a number of other ligand/receptor systems to influence how cells translate environmental signals to orchestrate morphogenetic events. (silverchair.com)
  • protein tyrosine phosphatase, non-receptor. (wikigenes.org)
  • We have applied this method to the development of proteins that bind epidermal growth element receptor (EGFR) website II. (healthyconnectionsinc.com)
  • The AF-1 plays an important role in the interaction of the receptor with molecules necessary for the initiation of transcription, such as coactivators, chromatin modulators, and basal transcription factors, including RNA polymerase II, TATA-binding protein (TBP), and a host of TBP-associated proteins (TAFIIs). (medscape.com)
  • We also have collaborative interests in determining structures of protein complexes formed with anti-cancer and anti-thrombosis inhibitors. (nottingham.ac.uk)
  • AbstractHerpesviruses are known to encode a number of inhibitors of host cell death, including Rip Homotypic Interaction Motif (RHIM)-containing proteins. (howhelp.org)
  • The Pro-Ile-Asn-Ile-Thr (PINIT) motif represents a highly conserved region of PIAS proteins, which was shown to be involved in the nuclear retention of PIAS3. (wikipedia.org)
  • The targeting motif of Ras is part of the COOH-terminal hypervariable region, which significantly differs amongst the otherwise highly conserved Ras isoforms. (rupress.org)
  • Programmed Cell Death protein (Pdcd) 4 is a highly conserved nuclear/cytoplasmic shuttling and RNA binding protein that has been shown to suppress tumour progression by regulating transcription and translation and is therefore considered a promising target for anti-cancer therapies. (le.ac.uk)
  • Mechanisms by which E. chaffeensis establishes intracellular infection, and avoids host defenses are not well understood, but involve functionally relevant host-pathogen interactions associated with tandem and ankyrin repeat effector proteins. (frontiersin.org)
  • Pharmacological USP7 inhibition by P5091 retarded cell proliferation and induced cell apoptosis. (biomedcentral.com)
  • USP7 inhibition induces cell apoptosis and cell cycle G2/M arrest, and overcomes taxane resistance by inducing the protein degradation of PLK1, resulting in chromosome misalignment in mitosis. (biomedcentral.com)
  • USP7 specifically dequbiquitinates and stabilizes both p53 and MDM2 to various degrees, and USP7 inhibition is expected to inactivate MDM2 and activate p53, thereby leading to cell cycle arrest or apoptosis in cancer cells with functional p53 signaling [ 10 ]. (biomedcentral.com)
  • Whereas other herpesvirus RHIMs inhibit necroptosis, this new VZV RHIM targets the host RHIM-containing protein ZBP1 to inhibit apoptosis during infection. (howhelp.org)
  • This is the first study to demonstrate the importance of the ZBP1 pathway in VZV infection and to identify the role of a viral RHIM in apoptosis inhibition. (howhelp.org)
  • Within the PINIT domain, the PIAS382-132 region was isolated and characterized as an inhibitory domain that binds and inhibits both the MITF and STAT3 transcription factors. (wikipedia.org)
  • We are interested in RNA structure, dynamics, and folding, and are currently investigating the properties of the Varkud Satellite Virus Ribozyme Stemloop V and the 60 nucleotide fragment of 23S rRNA that binds the L11 protein. (wustl.edu)
  • It binds both the U1 snRNA Stemloop II and the U2 snRNA Stemloop IV, and also binds the U2A' protein. (wustl.edu)
  • When blood vessels are damaged by a cut or by other types of vascular injury, VWF in blood plasma binds to the site of damage and unravels to form long protein strings that specifically capture specialised blood cells (platelets) to the site of injury. (nottingham.ac.uk)
  • In UPS-induced protein degradation, ubiquitin binds to target proteins and catalyzes them by a hierarchical cascade comprising E1, E2 and E3 ubiquitin ligases [ 2 ]. (biomedcentral.com)
  • The chromo-domain binds histone H3 tails methylated on lysine 9. (cipsm.de)
  • This study reports that LKB1 binds to Phosphoinositide-dependent kinase (PDK1) by a conserved binding motif. (sdbonline.org)
  • Further, CIP2A competes with B56α substrate binding by blocking the LxxIxE-motif substrate binding pocket on B56α. (tuni.fi)
  • Inhibition of cell/substrate adhesion with specific anti-integrin antibodies demonstrated that P-FAKSer732 activation is not induced by integrin clustering. (aacrjournals.org)
  • This is the first report that PLK1 is a novel substrate of USP7 deubiquitinase, and that USP7 sustained the protein stability of PLK1. (biomedcentral.com)
  • DUBs are critical in cellular growth, survival and homeostasis, and are responsible for the turnover, localization and activity of their substrate proteins. (biomedcentral.com)
  • The assembly of PRMT6 protein network allowed us to formulate functional hypotheses which led to the discovery of new molecular partners for the architectural transcription factor HMGA1a, a known substrate for PRMT6, and to provide evidences for a modulatory role of HMGA1a on the methyltransferase activity of PRMT6. (embl.de)
  • This structure unveils the molecular basis for the interaction and cooperativity between the whole replicative polymerase and PCNA with an unprecedented level of detail. (pasteur.fr)
  • Despite its small genome and limited number of effector proteins, Ehrlichia efficiently establishes an intracellular infection and avoids immune defenses in vertebrate and invertebrate hosts through complex molecular and cellular reprogramming strategies. (frontiersin.org)
  • Thus, E. chaffeensis is an excellent model organism to study host-pathogen interactions and to understand the molecular pathobiology of obligately intracellular microbes. (frontiersin.org)
  • We performed in silico threedimensional modeling of apoptin, molecular docking experiments between apoptin model and the known structure of Bcr- Abl, and the 3D structures of SH2 domains of CrkL and Bcr-Abl. (mun.ca)
  • The findings show how a crucial enzyme in our blood, known as ADAMTS13, works like a pair of molecular scissors to carefully cut back the clotting effects of a key protein, von Willebrand factor (VWF). (nottingham.ac.uk)
  • Identification and characterization of new molecular partners for the protein arginine methyltransferase 6 (PRMT6). (embl.de)
  • MDM2 is a multidomain protein that functions as an E3 ubiquitin ligase, transcription repressor, mRNA-binding protein, translation factor, and molecular chaperone. (ed.ac.uk)
  • We conclude that the polybasic-prenyl motif acts as a Ca 2+ /CaM-regulated molecular switch that controls PM concentration of KRas and redistributes its activity to internal sites. (rupress.org)
  • Research interests include determining protein structures and studying the structure/function of complexes formed with drugs and natural ligands. (nottingham.ac.uk)
  • Adhesion complexes (i.e., focal adhesions and focal complexes) and cell-cell contacts are specialized structures that harbor a large number of cytoskeletal proteins and one of the highest concentrations of signaling molecules in cells ( Ruoslahti and Obrink, 1996 ). (rupress.org)
  • Complexes stabilized by intermolecular interactions involving RHIMs have a fibrillar amyloid structure. (howhelp.org)
  • We have identified a novel RHIM within the ORF20 protein expressed by Varicella zoster virus (VZV) that forms amyloid-based complexes with human cellular RHIMs. (howhelp.org)
  • Structures of Hepatitis B Virus Core- and e-Antigen Immune Complexes Suggest Multi-point Inhibition. (nih.gov)
  • This process is carried out by the human spliceosome machinery, in which over 300 proteins sequentially assemble with uridine-rich small nuclear RNA molecules (U snRNAs) to form distinct small nuclear ribonucleoprotein complexes (snRNPs). (biomedcentral.com)
  • Complexes of Vps34, the sole class III PI3K member, produce PI 3-phosphate (PI(3)P) in the endolysosomal system and during autophagy to regulate vesicle-mediated sorting en route to lysosomes 1 . (nature.com)
  • The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)
  • These elements are frequently found near gene enhancers and interact with nuclear matrix proteins to provide a unique nuclear microenvironment for transcriptional regulation. (wikipedia.org)
  • This signature motif has been shown to mediate interactions between nuclear receptors and their co-regulators. (wikipedia.org)
  • The bacterial sliding clamp is referred to as the b clamp, while the eukaryotic and archaeal sliding clamp protein is called the proliferative cell nuclear antigen (PCNA). (pasteur.fr)
  • Apoptin attributes such as SH2-like sequence similarity with CrkL SH2 domain, unique SH3 domain binding sequence, presence of proline-rich segments, and its nuclear affinity render the molecule capable of interaction with Bcr-Abl. (mun.ca)
  • Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. (affbiotech.cn)
  • The protease not only releases small peptides, such as the amyloid-β peptide, which drives Alzheimer's disease pathogenesis, but also intracellular domains, which can have critical functions in nuclear signaling. (cipsm.de)
  • Rev interacts with Crm1 through a leucine-rich nuclear export signal located in its C-terminus, and with RRE through an Arginine rich motif (ARM) located in the N-terminal region. (nih.gov)
  • A new HIV-1 Rev structure optimizes interaction with target RNA (RRE) for nuclear export. (nih.gov)
  • For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
  • The PIAS family contains more than 60 proteins, most of them transcription factors that can be either positively or negatively regulated through multiple mechanisms. (wikipedia.org)
  • p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. (affbiotech.cn)
  • Canonical Notch signaling activates the transcription of BMI1 proto‑oncogene polycomb ring finger, cyclin D1, CD44, cyclin dependent kinase inhibitor 1A, hes family bHLH transcription factor 1, hes related family bHLH transcription factor with YRPW motif 1, MYC, NOTCH3, RE1 silencing transcription factor and transcription factor 7 in a cellular context‑dependent manner, while non‑canonical Notch signaling activates NF‑κB and Rac family small GTPase 1. (spandidos-publications.com)
  • The heterochromatin-enriched HP1 proteins play a critical role in regulation of transcription. (cipsm.de)
  • To test our hypothesis that histone H4R3 and H3R17 methylation regulates ferritin transcription, H4R3 and H3R17 protein arginine (R) methyltransferases 1 and 4 (PRMT1 and PRMT4) were investigated. (embl.de)
  • His work on RNA binding proteins and SRSF1 has been continuously funded by the NIH and has recently expanded to the study of the role played by multiple RNA binding proteins on cellular gene transcription and T cell development and functions. (fau.edu)
  • The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
  • E3 SUMO-protein ligase PIAS3 is an enzyme that in humans is encoded by the PIAS3 gene. (wikipedia.org)
  • The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. (cancerindex.org)
  • What does this gene/protein do? (cancerindex.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • Gene expression is known to be affected by interactions between local genetic variation and DNA accessibility, with the latter organized into three-dimensional chromatin structures. (elifesciences.org)
  • With 176 samples profiled for genotype, gene expression, and open chromatin, we used regression modeling to infer genetic-epigenetic interactions on a genome-wide scale. (elifesciences.org)
  • We found that these interactions occur within the local area of the affected gene, and that this locality corresponds to topologically associated domains (TADs). (elifesciences.org)
  • Local regulatory mechanisms within the genome and their interaction with chromatin structure give rise to subtle variations in gene expression. (elifesciences.org)
  • Understanding the scope and landscape of these interactions on a genome-wide scale is a vital step towards deciphering the genetic regulation of gene expression and, in turn, the mechanisms of non-coding variation on phenotypic outcomes. (elifesciences.org)
  • Transcriptional regulation of the human ferritin gene by coordinated regulation of Nrf2 and protein arginine methyltransferases PRMT1 and PRMT4. (embl.de)
  • 2017) Tat is a multifunctional viral protein that modulates cellular gene expression and functions. (fau.edu)
  • 2015) SRSF1 inhibition of HIV-1 gene expression. (fau.edu)
  • The current approaches to develop novel protein relationships are based on gene duplication and gene changes [1]. (healthyconnectionsinc.com)
  • in an operon with an upstream PurR/LacI-type transcriptional regulator gene, named amlR ( ACSP50_2475 ), and a gene downstream ( ACSP50_2473 ) encoding a GGDEF-EAL-domain-containing protein putatively involved in c-di-GMP signaling. (frontiersin.org)
  • The M2 is a transmembrane protein that forms an ion channel required for the uncoating process that precedes viral gene expression. (cdc.gov)
  • Here, we report on the clinical impact of a novel NiV-derived nonspreading replicon particle lacking the fusion (F) protein gene (NiVF) as a vaccine in three small animal models of disease. (cdc.gov)
  • [ 1 ] His prediction was subsequently supported by the cloning of the retinoblastoma tumor suppressor gene ( RB1 ) and by functional studies of the retinoblastoma protein, Rb. (medscape.com)
  • However, a detailed mechanistic understanding of functional PCNA:ligase I interactions has been incomplete. (nih.gov)
  • Together these results demonstrate the functional significance of the beta-zipper structure formed between the C-terminal domain of PCNA and Cdc9 and reveal differences in the interactions of FEN-1 and Cdc9 with the two PCNA interfaces that may contribute to the co-ordinated, sequential action of these enzymes. (nih.gov)
  • They investigated if Delta-ORF7a Δ17del produced a detectable/functional protein, the site of ORF7a localization in infected cells and assessed ORF7aΔ 17del growth in vitro . (news-medical.net)
  • It has several distinct functional domains in addition to the Abl kinase domain. (mun.ca)
  • By resolving the crystal structure of the functional domains of ADAMTS13, the research reveals how after binding VWF, the enzyme must change its shape to open the active site and in turn specifically accommodate the cleavage site in VWF. (nottingham.ac.uk)
  • Nrf2 consists of six functional Neh domains (Neh1-Neh6), from which, the amino-terminal Neh2 domain controls binding Keap1-the inhibitor protein Kelch-like ECH-associated protein 1, that is responsible for the cytosolic sequestration of Nrf2 under physiological conditions (Fig. 2 a). (springer.com)
  • Here we demonstrate that PSRP1 is not a bona fide ribosomal protein, but rather a functional homologue of the Escherichia coli cold-shock protein pY. (cipsm.de)
  • We describe the residue-specific methylation of E2F-1 by the asymmetric dimethylating protein arginine methyltransferase 1 (PRMT1) and symmetric dimethylating PRMT5 and relate the marks to different functional consequences of E2F-1 activity. (embl.de)
  • B) Functional domains of the hGR-alpha. (medscape.com)
  • The functional domains and subdomains are indicated beneath the linearized protein structures. (medscape.com)
  • Rip homotypic interaction motifs (RHIMs) are found in host proteins that can signal for programmed cell death and in viral proteins that can prevent it. (howhelp.org)
  • Rev has been reported to interact with many host proteins including importin-ß, histone chaperons (Nap1 and B23), and tubulin through its ARM domain. (nih.gov)
  • The RLD domain is also involved in the positive regulation of SMAD3 by PIAS3. (wikipedia.org)
  • Collectively, we discover a unique multi-step hijack and mute protein complex regulation mechanism resulting in tumour suppressor PP2A-B56α inhibition. (tuni.fi)
  • RNA and RNA:Protein interactions provide biological regulation at all levels of cellular function. (wustl.edu)
  • Plastid-specific ribosomal proteins (PSRPs) have been proposed to play roles in the light-dependent regulation of chloroplast translation. (cipsm.de)
  • PRMT6 is a protein arginine methyltransferase that has been implicated in transcriptional regulation, DNA repair, and human immunodeficiency virus pathogenesis. (embl.de)
  • Chromatin target of Prmt1 (Chtop) is a vertebrate-specific chromatin-bound protein that plays an important role in transcriptional regulation. (embl.de)
  • Until recently regulation of translation inhibition was exclusively thought to be eIF4A mediated, and was shown to involve the interaction of the tandem MA3 domains of Pdcd4 with the RNA helicase, eIF4A. (le.ac.uk)
  • Similar to the functionally homologous human proteins, yeast RFC interacts with and inhibits Cdc9 DNA ligase whereas the addition of PCNA alleviates inhibition by RFC. (nih.gov)
  • USP7 interacts with PLK1 protein through its PBD domain by catalytic activity. (biomedcentral.com)
  • The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. (cipsm.de)
  • Pertussis toxin B-pentamer mediates intercellular transfer of membrane proteins and lipids. (uc.edu)
  • During her postdoctoral studies at the University of Massachusetts Medical School (UMASS), she focused on the structure and function of outer membrane proteins, mainly Occ-family of porins from P. aeruginosa , and their role in bacterial pathogenesis and antibiotic resistance. (nih.gov)
  • Here we report the selection of camelid-derived single-domain antibodies (nanobodies) that modulate the conformation and spectral properties of the green fluorescent protein (GFP). (cipsm.de)
  • To mimic the evolutionary process by which protein networks develop, we adopted the basic mechanism by which antibodies are produced against antigens. (healthyconnectionsinc.com)
  • The hemagglutinin (HA), neuraminidase (NA), and M2 are essential viral proteins targeted by host antibodies or antiviral drugs such as oseltamivir and rimantadine ( 4 - 6 ). (cdc.gov)
  • Keap1 is a cysteine-rich protein, known to be anchored to actin cytoskeleton [ 5 ], serving as an adaptor protein for the Cul3-dependent E3 ubiquitin ligase complex. (springer.com)
  • The ectodomain contains a globular ligand binding domain (LBD), a cysteine-rich region with a Sushi and an epidermal growth factor (EGF)-like domain, and two fibronectin type III domains (FN1 and FN2). (silverchair.com)
  • Two critical functions of IE1 are inhibition of SUMOylation of Promyelocytic leukemia protein (PML) and transactivation of viral promoters. (ncbs.res.in)
  • Although the non-covalent interaction of IE1 and SUMO is not involved in the inhibition of PML SUMOylation, it contributes to the transactivation activity. (ncbs.res.in)
  • Our results suggest that transactivation and inhibition of PML SUMOylation are independent activities of IE1. (ncbs.res.in)
  • The NTD of the hGRα contains a major transactivation domain, termed activation function (AF)-1, which is located between amino acids 77 and 262 of the hGRα and is ligand-independent. (medscape.com)
  • The LXXLL motif represents the minimal requirement for the interaction with the NFκB p65 subunit and for the inhibition of NFκB transcriptional activity. (wikipedia.org)
  • Consistent with evidence in flies, neurospheres from patients with high V-ATPase subunit expression show inhibition of autophagy. (sdbonline.org)
  • Here we present the co-crystal structure of yeast PCNA with a peptide encompassing the conserved PCNA interaction motif of Cdc9, yeast DNA ligase I. The Cdc9 peptide contacts both the inter-domain connector loop (IDCL) and residues near the C-terminus of PCNA. (nih.gov)
  • FN2 is followed by a transmembrane (TM) helix, and an intracellular part consisting of a juxtamembrane (JM) region with several conserved tyrosine (Y) residues, a tyrosine kinase domain, a sterile-α motif (SAM) protein-protein interaction domain, and a C-terminal Psd-95, Dlg and ZO1 domain (PDZ)-binding motif ( Pasquale, 2008 ). (silverchair.com)
  • However, another dominant suppression of H/D exchange was observed in a motif from amino acids 103-107 that reflects surface hydrophobic residues surrounding the hydrophobic pocket of MDM2. (ed.ac.uk)
  • Following the evaluation of the model and the identification of motifs characteristic of the Flaviviridae family, data from previous publications on the treatment of HCV were incorporated, with the aim of detecting the ideal residues in the Spondweni model, which are similar to those of the HCV structure and are inhibitor targets. (spandidos-publications.com)
  • Here we show that FIC-domain-mediated adenylylation is controlled by a conserved mechanism of ATP-binding-site obstruction that involves an inhibitory α-helix (α(inh)) with a conserved (S/T)XXXE(G/N) motif, and that in this mechanism the invariable glutamate competes with ATP γ-phosphate binding. (rcsb.org)
  • After mutation of the inhibitory motif in various Fic proteins, including the human homologue FICD (also known as HYPE), adenylylation activity is considerably boosted, consistent with the anticipated relief of inhibition. (rcsb.org)
  • Expression of the Escherichia coli tryptophanase operon depends on ribosome stalling during translation of the upstream TnaC leader peptide, a process for which interactions between the TnaC nascent chain and the ribosomal exit tunnel are critical. (cipsm.de)
  • However, the authentic p53-derived peptide:MDM2(Y104G) complex exhibited partial resistance to Nutlin-3 inhibition, while the p53-mimetic 12.1 peptide:MDM2(Y104G) complex retained normal Nutlin-3 responsiveness. (ed.ac.uk)
  • The protein interaction of PLK1 and USP7 was detected by tandem affinity purification and high throughput proteomics, and further confirmed by co-immunoprecipitation, GST pull-down and protein co-localization. (biomedcentral.com)
  • We are primarily using cytomegaloviruses to examine how the pathogens alter signaling pathways directed by G-protein coupled receptors (GPCRs) to facilitate robust replication in tissues important for host-host dissemination. (uc.edu)
  • This is a prerequisite to finely analyze how these pathways are integrated in the cell machinery and to study their role in cancer and other human diseases when this network of interactions is perturbed. (oncotarget.com)
  • Furthermore, a PDK1-consensus motif is located within the kinase domain of LKB1 and LKB1 gets phosphorylated by PDK1 in vitro. (sdbonline.org)
  • Future studies should reveal how intrinsic or extrinsic factors modulate adenylylation activity by weakening the interaction of α(inh) with the FIC active site. (rcsb.org)
  • This was followed-up in his post-doctoral research at UC Santa Cruz (1996-2001) where he was awarded the prestigious Human Frontier Science Program fellowship and published breakthrough work characterizing the key RNA sequences and the cellular proteins that modulate the alternative splicing of the HIV-1 messenger RNA (mRNA). (fau.edu)
  • During his tenure at FAU, Dr. Caputi has developed novel techniques to study RNA - RNA binding proteins interactions and made important contributions to our understanding of how cellular proteins modulate the replication of the HIV-1 genome. (fau.edu)
  • Therefore, the oncogenic effect of Bcr-Abl could be inhibited by the interaction of small molecules with these domains. (mun.ca)
  • RNA molecules and their interactions with protein partners in order to understand their biology. (wustl.edu)
  • Cell adhesion molecules are a diverse group of proteins that play a crucial role in mediating cell-to-cell interactions and cell-to-extracellular matrix interactions. (pharmiweb.com)
  • The Human Cytomegalovirus (HCMV) Immediate-Early protein 1 (IE1) is the first viral protein to express during infection. (ncbs.res.in)
  • Apoptin is a viral protein with well-documented cancer-selective cytotoxicity. (mun.ca)
  • She currently studies the underlying mechanisms of viral pathogenesis, viral protein-host protein interactions, and the potential use of humanized scFvs and nanobodies for treatment. (nih.gov)
  • Organisms within the archaeal domain of life possess a simplified version of the eukaryotic DNA replication machinery. (pasteur.fr)
  • Several agents have been developed to target these key regulatory proteins that are essential for HIV replication. (biomedcentral.com)
  • The HIV-1 Rev protein is a key regulatory factor that is essential for both early and late phases of viral replication cycles, and therefore represents an important viral target for drug development. (nih.gov)
  • The latter region of NS proteins, at the C-terminal part of the polyprotein, has a great contribution in the RNA replication process. (spandidos-publications.com)
  • Being a promising antiviral target, helicase inhibition leads to the pause of the replication, proliferation and consequently, to the survival and transmission of Flaviviridae viruses ( 3 ). (spandidos-publications.com)
  • The protein phosphatase 2A (PP2A) heterotrimer PP2A-B56α is a human tumour suppressor. (tuni.fi)
  • ΔNp63α has been reported to transcriptionally inhibit the tumour suppression protein p53. (researchsquare.com)
  • We found that TRIM13 mRNA and protein expression was reduced in NSCLC tissues and cell lines in comparison to paired non-cancerous tissues and a human normal bronchial epithelial cell line, respectively. (cancerindex.org)
  • Alternative splicing of the primary transcript gives rise to the 2 mRNA and protein isoforms, hGR-alpha and hGR-beta. (medscape.com)
  • After activation of different receptors at the cell surface, this dimeric protein serves as a platform for assembling other adaptors such as FYB and some SRC family kinase members, although these mechanisms are still poorly understood. (oncotarget.com)
  • This evolutionarily conserved domain is found in proteins ranging from yeast to human and is shared by other chromatin-binding proteins, such as scaffold attachment factor A and B. The SAP domain can recognize and bind to AT-rich DNA sequences present in scaffold-attachment regions/matrix-attachment regions. (wikipedia.org)
  • Typically, EphA receptors bind to glycosylphosphatidylinositol anchor (GPI)-linked ephrin A proteins and EphB receptors bind to transmembrane ephrin B proteins. (silverchair.com)
  • The small molecule Nutlin-3 has been engineered to bind to the N-terminal hydrophobic pocket domain of MDM2. (ed.ac.uk)
  • For the evaluation of the accuracy and reliability of the model in structure‑based drug design strategies, the crystal structure of the hepatitis C virus (HCV) helicase was used, complexed with a single‑stranded RNA, a key molecule for the establishment of interactions with a future inhibitor of the SPONV helicase. (spandidos-publications.com)
  • The most crucial NS proteins are the viral helicase and the viral RNA-dependent RNA polymerase (RdRp) ( 2 ). (spandidos-publications.com)
  • PIAS3 protein also functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. (wikipedia.org)
  • In addition to the autophagy-lysosome system, the ubiquitin-proteasome system (UPS) takes up approximately 80 to 90% of intracellular protein degradation [ 1 ]. (biomedcentral.com)
  • USP7 was originally identified as a binding partner for the herpes simplex virus (HSV) infected cell protein and named herpes-associated ubiquitin-specific protease (HAUSP) [ 6 ]. (biomedcentral.com)
  • Thus, vinculin regulates survival and motility via ERK by controlling the accessibility of paxillin for FAK interaction. (rupress.org)
  • Simultaneous hyperactivation of stress-activated protein kinase/c-Jun N-terminal protein kinase (SAPK/JNK) and mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (MEK/ERK) signaling cascades has been reported in carcinogenesis. (bvsalud.org)
  • When Stemloop IV is bound to SNF, the affinity for U2A' protein is enhanced, and when U2A' is bound to SNF, the affinity for SLIV is increased. (wustl.edu)
  • However, even though scaffolds constructed in these studies have shown affinity to numerous focuses on, the selection of different protein frameworks specific to a predetermined target surface patch has not been successful except in a recent study that developed protein binders for influenza hemagglutinin [20]. (healthyconnectionsinc.com)
  • This machinery recognizes pre-mRNA sequences at several motifs - the 5′ and 3′ splice sites, the branch point, and polypyrimidine tracts - positioned at exon-intron boundaries [ 4 ]. (biomedcentral.com)
  • After CD4 binding, various gp120 domains interact with the enzyme PDI and the chemokine co-receptors forms a PDI-CD4-gp120-chemokine complex. (biomedcentral.com)
  • In this study we have calculated a 3D structure of apoptin and through modeling and docking approaches, we show its interaction with Bcr-Abl oncoprotein and its downstream signaling components, following which we confirm some of the newly-found interactions by biochemical methods. (mun.ca)
  • It has two main isoforms: TAp63 has an N-terminal TA homologous to that of p53, while ΔNp63 has a truncated but specific TA domain, the expression of which depends on selective promoters. (researchsquare.com)
  • In the first step, a virtual testing of a human protein scaffold library is definitely carried out to determine a platform specific to the surface patch of interest. (healthyconnectionsinc.com)
  • Target specific-scaffolds with designs complementary to the surface patch of interest are selected from your scaffold library through protein docking simulations (top right). (healthyconnectionsinc.com)
  • Microtubule-associated Ser/Thre kinase 2 (MAST2), inhibits neurite outgrowth, and its inhibition therefore represents a potential therapeutic strategy. (hal.science)
  • It was previously described that the LXXLL motif is also responsible for the retention of PIAS3 in the nucleus. (wikipedia.org)
  • These proteins are vital to normal platelet adhesion and hemostatic function. (nottingham.ac.uk)
  • Some tumor suppressor proteins are involved in maintaining cell adhesion, preventing cells from breaking away and spreading to other parts of the body (metastasis). (pharmiweb.com)
  • This binding of Nutlin-3 has two consequences: (i) antagonistic effects through competitive disruption of the MDM2-p53 complex and (ii) agonist effects that allosterically stabilize MDM2 protein-protein interactions that increase p53 ubiquitination as well as nucleophosmin deoligomerization. (ed.ac.uk)
  • We present a methodology using a hydrogen/deuterium (H/D) exchange platform that measures Nutlin-3 binding to the N-terminal domain of MDM2 (MDM2(1-126)) in order to begin to develop dynamic assays that evaluate MDM2 allostery. (ed.ac.uk)
  • In order to explore the consequences of this latter Nutlin-3 interaction site on MDM2, the Y104G and L107G mutant series was constructed. (ed.ac.uk)
  • The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)
  • It is a multifunctional and conditionally essential protein for HCMV infection. (ncbs.res.in)
  • Analyses of these interactions has previously been limited, obscuring their regulatory context, and the extent to which they occur throughout the genome. (elifesciences.org)
  • This has led to the concept of a "local area" in which most regulatory interactions take place. (elifesciences.org)
  • Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. (cipsm.de)
  • Oxidative stress generated during such stressful conditions may damage DNA and proteins, and as a consequence the cellular processes are disturbed. (springer.com)
  • Despite the numerous, well-described functions and interactions of TDP-43, it is not well understood exactly which TDP-43-dependent cellular processes become defective in ALS/FTD and contribute to disease etiology. (nature.com)
  • Protein stability is critical for normal cellular homeostasis. (biomedcentral.com)
  • Trans interactions of Eph receptors with ephrins at cell-cell interfaces promote a variety of cellular responses, including repulsion, attraction and migration. (silverchair.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Many tumor suppressor proteins play a role in DNA repair, helping to correct genetic mutations and maintain the stability of the genome. (pharmiweb.com)
  • The AF form of SALSA had a more intact structure and contained peptides from the zona pellucida domain, which is involved in cell differentiation and oligomerization. (researchgate.net)
  • The human GR is a modular protein composed of distinct regions illustrated in panel B in the image below, as follows: (1) The amino-terminal A/B region, also called immunogenic or N-terminal domain (NTD) and (2) the C, D, and E regions, which correspond to the DNA-binding domain, the hinge region, and the ligand-binding domain, respectively. (medscape.com)
  • The 3 viral envelope proteins of influenza A virus are most medically relevant. (cdc.gov)
  • After her postdoctoral studies, Dr. Eren joined the National Institute of Allergy and Infectious Diseases (NIAID) at NIH as a research fellow where she studied host immune system manipulation by Rotavirus proteins. (nih.gov)
  • Relevant to oncogenic activity of CIP2A across human cancers, the N-terminal head domain-mediated interaction with B56α stabilizes CIP2A protein. (tuni.fi)
  • Our work has focused for some time on human U1A protein and its recognition of two RNAs. (wustl.edu)
  • We have developed a strategy using protein docking simulation that imitates this process of antibody generation to select human being protein scaffolds with complementary designs (Number 1B). (healthyconnectionsinc.com)
  • This procedure designs novel protein interactions by selecting human protein scaffolds with designs that match a predetermined surface patch on a target protein (Number 1C). (healthyconnectionsinc.com)
  • By analyzing breast invasive carcinoma database (The Cancer Genome Altas), we found that the mRNA expression levels of both JNK1 and ERK2 are positively correlated with the mRNA level of EEA1, an endosome associated protein, indicating the potential JNK/ERK crosstalk at endosome. (bvsalud.org)
  • Here we undertake a genome-scale analysis of these interactions in a genetically diverse population to systematically identify global genetic-epigenetic interaction, and reveal constraints imposed by chromatin structure. (elifesciences.org)
  • Our results demonstrate that statistical interactions between genetic variants and chromatin accessibility are common throughout the genome. (elifesciences.org)