• Ganoderma lucidum inhibits proliferation and induces apoptosis in human prostate cancer cells PC-3. (greenmedinfo.com)
  • In this study, we investigated the effects of Ganoderma lucidum on cell proliferation, cell cycle, and apoptosis in human prostate cancer cells PC-3. (greenmedinfo.com)
  • Furthermore, Ganoderma lucidum induced apoptosis of PC-3 cells with a slight decrease in the expression of NF-kappaB-regulated Bcl-2 and Bcl-xl. (greenmedinfo.com)
  • In addition, cell cycle distribution analysis revealed that G0/G1 phase arrest was induced following pristimerin treatment in CAL‑27 and SCC‑25 cells, which was strongly associated with upregulation of p21 and p27, coupled with downregulation of cyclin D1 and cyclin E. Meanwhile, pristimerin induced significant apoptosis of CAL‑27 and SCC‑25 cells, alongside decreased levels of caspase‑3 and specific cleavage of poly (ADP‑ribose) polymerase. (spandidos-publications.com)
  • Pristimerin has also been reported to induce apoptosis of various human cancer cells, including in multiple myeloma ( 10 ), breast ( 11 ), liver ( 12 ), pancreatic ( 13 ) and prostate cancer ( 14 ). (spandidos-publications.com)
  • In addition to apoptosis induction ( 11 ), the mechanisms involved in the anticancer effects of pristimerin include stimulation of reactive oxygen species generation ( 15 ), blocking of nuclear factor-κB ( 16 ) and proteasome inhibition ( 10 ). (spandidos-publications.com)
  • Pristimerin exhibited potent anti-proliferative and apoptosis-inducing effects on the OSCC cell lines CAL-27 and SCC-25. (spandidos-publications.com)
  • New anticancer therapy strategies refer to the inhibition of CDK-cyclin complexes as an important target to prevent uncontrolled proliferation and induce apoptosis in cancer cells ( 2 ). (spandidos-publications.com)
  • Although purvalanol-induced cell cycle arrest and apoptotic cell death were demonstrated in prostate ( 5 ), breast ( 6 ) and colon cancer cells ( 7 ), the exact molecular mechanism of purvanol-induced apoptosis has not been elucidated yet. (spandidos-publications.com)
  • The inhibition of cell proliferation was related to arrest of the cell cycle in the G0/G1 phase and increased apoptosis in response to PTGR1 knockdown as indicated by flow cytometry. (go.jp)
  • Quercetin also improve the action of the drug 5-Fluorouracil (5-FU) promoting increased expression of p53 and apoptosis in breast cancer T47D cells [ 6 , 7 ]. (biomedcentral.com)
  • The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
  • Tan IIA inhibited proliferation of mouse keratinocytes in a dose- and time-dependent manner and induced apoptosis, resulting in S phase arrest accompanied by down-regulation of pCdk2 and cyclin A protein expression. (hindawi.com)
  • Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
  • Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
  • Targeting this pathway with a small molecule inhibitor, LCRF-0004, resulted in decreased proliferation with a concomitant increase in apoptosis. (frontiersin.org)
  • Recombinant MST1 treatment was unable to overcome the effect of LCRF-0004 in terms of either proliferation or apoptosis. (frontiersin.org)
  • Cell cycle distribution and apoptosis were detected by flow cytometric analysis and MTT analysis. (scienceopen.com)
  • The inhibition of RDM1 expression by RNAi significantly impaired cell proliferation, increased apoptosis and arrested cells in the G2/M phase. (scienceopen.com)
  • Moreover, RDM1 may play an important role in cell proliferation, cell cycle distribution and apoptosis of human PTC cells. (scienceopen.com)
  • As a consequence, any modulations of the target transcript expression, miRNAs can affect various signalling pathways and cellular process such as apoptosis, proliferation or differentiation. (ukessays.com)
  • Effect of cadmium on P53 and mitogen-activated protein kinases in a murine macrophage cell line: relation to apoptosis. (cdc.gov)
  • It is immunotoxic in vivo and induces apoptosis in many cultured cells. (cdc.gov)
  • Present study was designed to determine the impact of cadmium on cell proliferation, cell cycle and apoptosis, and to investigate the possible involvement of p53 and MAPKs signaling pathways in J774A.1 murine macrophages. (cdc.gov)
  • Cd inhibited cell proliferation via cell cycle arrest and induced apoptosis in a dose-dependent manner. (cdc.gov)
  • Results suggested that p53 is not involved in Cd-induced cell cycle arrest and apoptosis in J774A.1 cells. (cdc.gov)
  • Further, AMP-17 treatment resulted in an increase in reactive oxygen species (ROS) levels, depolarization of the mitochondrial membrane potential (MMP), and changes in the cell cycle, leading to the apoptosis and necrosis, which ultimately contributed to the death of C. albicans cells. (researchsquare.com)
  • Additionally, JIB extract induced B16/F10 cell apoptosis via the caspase cascade. (medsci.org)
  • The results demonstrated that JIB extract combined with cisplatin enhanced the inhibition of cell growth, proliferation, and survival through the obstruction of cell cycle progression and AKT/mTOR and MAPK signaling as well as the induction of cell apoptosis. (medsci.org)
  • Effects of RAD001 and letrozole (alone and in combination) on the proliferation and survival of MCF7/Aro and T47D/Aro cells were evaluated using proliferation assays, flow cytometry, immunoblotting, and apoptosis analyses. (aacrjournals.org)
  • Melatonin inhibited cell growth and induced apoptosis in 5RP7 cells. (tubitak.gov.tr)
  • These findings show that melatonin induces cell death and apoptosis in H-ras oncogene transformed 5RP7 cells. (tubitak.gov.tr)
  • The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms. (biomedcentral.com)
  • While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. (biomedcentral.com)
  • The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene. (biomedcentral.com)
  • To this end, we overexpressed and inhibited gga-miR-155 in an MDV-transformed cell line using mimics and inhibitors respectively, and analyzed their growth, proliferation, apoptosis and invasiveness to explore the possible role of gga-miR-155 in MDV-mediated tumorigenesis. (biomedcentral.com)
  • Furthermore, we predicted and demonstrated that Retinoid Acid Receptor-Related Orphan Receptor Alpha (RORA) is one of the targets of gga-miR-155, and the gga-miR-155 regulated the proliferation, cell cycle, apoptosis and invasiveness of MSB1 cells by targeting RORA. (biomedcentral.com)
  • Flow cytometric analysis revealed partial cell arrest in the G(1) phase at less than 5 micro L/mL and induction of apoptosis at 10 micro L/mL or more of ethanol and water extracts, with the latter exhibiting more pronounced inhibition than the former. (nih.gov)
  • I'm-Yunity acts selectively in HL-60 leukemic cells, resulting in cell cycle restriction through the G(1)/S checkpoint and the induction of apoptosis. (nih.gov)
  • Flow cytometry was used to examine apoptosis, cell cycle distribution, and accumulation of reactive oxygen species (ROS). (jcancer.org)
  • Moreover, it induced cell cycle arrest in the G0/G1 phase, apoptosis, and ROS generation in these cells. (jcancer.org)
  • Silibinin considerably reduced the development of oral cancer cells by inducing apoptosis, G 0 /G 1 arrest, ROS generation, and activation of the JNK/c-Jun pathway. (jcancer.org)
  • Survivin is an apoptosis inhibitor that is associated with tumor proliferation and invasion. (springeropen.com)
  • Survivin belongs to the family of inhibitors of apoptosis proteins (IAPs) that plays a pivotal role in mitotic progression, apoptosis inhibition, angiogenesis, progression of cancer, and invasion [ 5 ]. (springeropen.com)
  • It is expressed in cancer cells during the G2/M phase of the cell cycle and counteracts apoptosis activation during mitosis by interfering with caspase activity [ 6 ]. (springeropen.com)
  • HIC was able to arrest the cell cycle at G1/S phase and also induced apoptosis and DNA damage, validated by gene expression profiling. (tuni.fi)
  • Cellular apoptosis (ie, programmed cell death) is another potential mechanism of many antineoplastic agents. (medscape.com)
  • Targeted inhibition of individual kinases of the MAPK signaling pathway using synthetic compounds represents a promising way to effective anti-cancer therapy. (mdpi.com)
  • However, THP-1 cells adaptively adjusted to LeTx and overrode cell cycle arrest by activating the PI3K/Akt signaling pathway. (uwo.ca)
  • To address the possibility that activation of phosphatidylinositol-3-kinase (PI3K) and the mammalian target of rapamycin (mTOR/FRAP), represents one of these pathways, we have examined the effect of simultaneous inhibition of the Ras-MAPK and PI3K-mTOR pathways on transformation of CEF by v-Src. (embl.de)
  • Similarly, inhibition of the PI3K-mTOR pathway by addition of the PI3K inhibitor 2-[4-morpholinyl]-8-phenyl-4H-1-benzopyran-4-one (LY294002) or the mTOR inhibitor rapamycin, although reducing several parameters of transformation, also failed to block transformation. (embl.de)
  • However, simultaneous inhibition of signaling by the Ras-MAPK pathway and the PI3K-mTOR pathway essentially blocked transformation. (embl.de)
  • In addition, AKT/mTOR signaling and MAPK signaling were inhibited by JIB extract to suppress melanoma cell growth and proliferation. (medsci.org)
  • RAD001 (everolimus), a mammalian target of rapamycin (mTOR) pathway inhibitor in phase II clinical trials in oncology, exerts potent antiproliferative/antitumor activities. (aacrjournals.org)
  • The role of the mTOR pathway in estrogen-driven proliferation and effects of combining RAD001 and letrozole were examined in vitro in two breast cancer models. (aacrjournals.org)
  • The role of the mTOR pathway in estrogen response was evaluated in aromatase-expressing MCF7/Aro breast cancer cells by immunoblotting. (aacrjournals.org)
  • Treatment of MCF7/Aro cells with estradiol or androstenedione caused modulation of the mTOR pathway, a phenomenon reversed by letrozole or RAD001. (aacrjournals.org)
  • To assess the effect of mammalian target of rapamycin (mTOR) inhibition on pituitary adenoma cells, rat GH3 or MMQ cells were treated with the clinically useful mTOR inhibitors, rapamycin or RAD001. (surgicalneurologyint.com)
  • Cellular proliferation and growth following exposure to mTOR inhibitors or radiation were assessed using biochemical methods. (surgicalneurologyint.com)
  • In the present study, we observed basal activation of mTOR, downstream of constitutive Akt signaling, in rat GH3 adenoma cells. (surgicalneurologyint.com)
  • Functionally, the mTOR inhibitors, rapamycin and RAD001 (500 pM-5 nM), induced G1 growth arrest within 24 hours, an effect associated with reduced cellular proliferation. (surgicalneurologyint.com)
  • Inhibition of mTOR also radiosensitized GH3 cells such that 2.5 Gy in combination with 500 pM rapamycin or RAD001 reduced cellular viability more effectively than 2.5 or 10 Gy alone. (surgicalneurologyint.com)
  • These data may support a possible therapeutic role for mTOR inhibitors in limiting the cellular proliferation and radioresistance of pituitary adenoma cells. (surgicalneurologyint.com)
  • 2 3 11 ] Based on our recent demonstration that basal Akt activation increased cellular growth in pituitary adenoma, we herein tested the hypothesis that mTOR activation, downstream from Akt signaling, may increase the growth and survival of pituitary adenoma cells. (surgicalneurologyint.com)
  • SOX9 promotes nasopharyngeal carcinoma cell proliferation, migration and invasion through BMP2 and mTOR signaling. (cancerindex.org)
  • In CAC, intestinal inflammation play critical role that induce DNA damage and cellular proliferation pathway. (biomedcentral.com)
  • We studied whether cellular senescence induced by HDAC inhibitor sodium butyrate in E1a+cHa-Ras -transformed rat embryo fibroblasts (ERas) and A549 human Ki-Ras mutated lung adenocarcinoma cells would enhance the tumor suppressor effect of MEK/ERK inhibition. (aging-us.com)
  • Treatment of control ERas cells with PD0325901 for 24 h results in mitochondria damage and apoptotic death of a part of cellular population. (aging-us.com)
  • This study demonstrated that modulation of PI3K/Akt/GSK3 signaling cascades can be beneficial for protecting or facilitating recovery from cellular LeTx intoxication in cells that depend on basal MEK1 activity for proliferation. (uwo.ca)
  • In human lymphocytes inhibition of IMPDH results in altered cellular proliferation with arrest in the S-phase of the cell cycle. (edu.sy)
  • 5-FU inhibits tumor cell growth through at least 3 different mechanisms that ultimately disrupt DNA synthesis or cellular viability. (medscape.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • tax causes malignant transformation not through integration and insertional mutagenesis, as many retroviruses do, but by inhibition of DNA repair (base excision pathway) and trans-activating disruption of cellular growth control mechanisms ( 2 ). (cdc.gov)
  • When applied to myoblasts, myostatin inhibits their proliferation and either initiates differentiation or stimulates quiescence. (wikipedia.org)
  • Adipose-derived MSCs, in particular, pose an attractive option for cell-based therapy due to their relatively decreased morbidity during isolation and potential for expansion and differentiation [ 12 ]. (hindawi.com)
  • Multipotential differentiation of MSCs into adipogenic, osteogenic, and chondrogenic cell lineages. (hindawi.com)
  • Excessive proliferation of keratinocytes, abnormalities in the differentiation process, and continuous shedding of the thickened epidermis are characteristics of psoriasis (Figures 1 (a) and 1 (b)) [ 2 ]. (hindawi.com)
  • It inhibits cytokine production and cell proliferation/differentiation in various immune cells. (uwo.ca)
  • Let-7 Let-7 has an essential role in cell cycle and the differentiation of cell cycle terminals. (ukessays.com)
  • Inhibition of EHMT2 expression or activity in human ERMS cell lines reduced DKK1 expression and elevated canonical Wnt signaling resulting in myogenic differentiation in vitro and in mouse xenograft models in vivo. (elifesciences.org)
  • Our study demonstrates that ERMS cells are vulnerable to EHMT2 inhibitors and suggest that targeting the EHMT2-DKK1-β-catenin node holds promise for differentiation therapy. (elifesciences.org)
  • Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)
  • Functional analysis revealed that knockdown of PTGR1 significantly inhibited proliferation and colony formation by PC cells. (go.jp)
  • To investigate the effects of silibinin on the growth of oral cancer cells, cell proliferation and anchorage-independent colony formation tests were conducted on YD10B and Ca9-22 oral cancer cells. (jcancer.org)
  • Silibinin effectively suppressed YD10B and Ca9-22 cell proliferation and colony formation in a dose-dependent manner. (jcancer.org)
  • A fairly similar Phase I/II study of sorafenib with anastrozole to overcome resistance to the aromatase inhibitors in patients with hormone receptor-positive MBC who had developed resistance to one of the aromatase inhibitors was presented at the 2009 SABCS. (medscape.com)
  • Given the modest-to-negligible activity of single-agent sorafenib in MBC, [ 49-50 ] the authors of the study felt that the benefit may be attributable to the restoration of sensitivity to aromatase inhibitors through inhibition of the Ras/Raf/MAPK pathway. (medscape.com)
  • Olomoucine, roscovitine and purvalanol are examples of CDK inhibitors (CDKIs) designed and investigated for their apoptotic potential on cancer cells ( 3 ). (spandidos-publications.com)
  • Despite a large number of promising inhibitors for Ras/Raf/MEK/ERK pathway, recent works show that cancer cells often develop an autophagy-dependent resistance to inhibitors of Ras pathway [ 16 ]. (aging-us.com)
  • Consistently, among 13 drugs targeting chromatin modifiers, EHMT2 inhibitors were highly effective in reducing ERMS cell viability. (elifesciences.org)
  • In this study, we explored the role of Plk1 in the classical kidney fibrosis model, UUO, and cultured fibroblast and renal tubular epithelial cells by using specific Plk1 inhibitors, genetic silencing, and heterozygous global knockout mice. (nature.com)
  • The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. (biomedcentral.com)
  • These effects were associated with inhibition of the mitogen‑activated protein kinase/extracellular signal‑regulated kinase 1/2 and protein kinase B signaling pathways. (spandidos-publications.com)
  • The underlying mechanisms of these effects were primarily mediated by G 1 phase cell cycle arrest and inhibition of the mitogen-activated protein kinase (MAPK)/extracellular signal-regulate kinase 1/2 (Erk 1/2) and protein kinase B (Akt) signaling pathways. (spandidos-publications.com)
  • Various physiological and pathological conditions such as glucose starvation, inhibition of protein glycosylation and oxidative stress may cause an accumulation of unfolded proteins in the endoplasmic reticulum (ER), leading to the unfolded protein response (UPR) and autophagy. (spandidos-publications.com)
  • Cyclin-dependent kinases (CDKs), are members of the serine-threonine protein kinase family and are responsible for taking control of cell cycle regulation in eukaryotic cells. (spandidos-publications.com)
  • 1) The regulation of these protein levels is vital for the understanding of the cell cycle control and its dysregulation. (ukessays.com)
  • 2) Therefore, the proper control of protein levels is crucial for the cell cycle. (ukessays.com)
  • The binding of miRNA- protein complexes to mRNA causes translation inhibition or destabilisation of target transcripts. (ukessays.com)
  • Cycloheximide, an inhibitor of protein synthesis, did not alter Cd-induced cytotoxicity indicating that J774A.1 cell death by Cd is independent of de novo protein synthesis including p53. (cdc.gov)
  • Plk1 protein and activity are low in G1, accumulate during S and G2/M phases, and are rapidly reduced in the late stages of mitosis [ 7 ]. (nature.com)
  • JIB extract induced cell cycle arrest at the G 0 /G 1 phase and decreased cyclin and cdk protein expressions. (medsci.org)
  • In cells, sirolimus binds to the immunophilin, FK Binding Protein-12 (FKBP-12), to generate an immunosuppressive complex. (newlystar-medtech.com)
  • P27 is a cyclin-dependent kinase inhibitor (CKI) that controls protein synthesis during the cell cycle. (springeropen.com)
  • P2Y1R-targeted HIC affects p53, MAPK and NF-κB protein expression, thereby improving the p53 stabilization essential for G1/S arrest and cell death. (tuni.fi)
  • The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. (cancerindex.org)
  • Procarbazine is an alkylating agent with a mechanism of action similar to that of dacarbazine: inhibition of DNA, RNA, and protein synthesis. (medscape.com)
  • Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
  • however, purvalanol triggered autophagy, which functions as a cell survival mechanism at early time-points. (spandidos-publications.com)
  • Macroautophagy (hereafter referred to as autophagy) plays an important role in cancer cells survival upon various conditions of intra- and extracellular stress. (aging-us.com)
  • Pretreatment with PD98059 increased Cd-induced cytotoxicity suggesting that ERK activation may be a survival response in the present cell system. (cdc.gov)
  • Survival, growth, and proliferation of malignant cells are affected by changes in both pro-apoptotic and anti-apoptotic signaling pathways. (springeropen.com)
  • In patients with relapsing or unresponsive disease, hematopoietic stem-cell transplantation significantly prolongs disease-free survival. (medscape.com)
  • Preclinical data have demonstrated that the combination of the aromatase inhibitor letrozole and sorafenib on breast cancer cells produced a synergistic inhibition of cell proliferation associated with a downregulation of the cell cycle regulatory proteins c-myc, cyclin D1 and phospho-Rb and possibly preventing the acquisition of resistance towards letrozole. (medscape.com)
  • Purvalanol, a novel cyclin-dependent kinase inhibitor, is referred to as a strong apoptotic inducer which causes cell cycle arrest in various cancer cells such as prostate, breast and colon cancer cell lines. (spandidos-publications.com)
  • Subsequently, the effect of an additional small molecular inhibitor, BMS-777607 (which targets MST1R (RON), MET, Tyro3, and Axl) also resulted in a decreased proliferative capacity of MPM cells. (frontiersin.org)
  • Inhibition of the Ras-MAPK pathway by expression of the dominant-negative Ras mutant HRasN17 or by addition of the MAPK kinase (MEK) inhibitor PD98059 reduced several of these parameters but failed to block transformation. (embl.de)
  • In this study we investigated the effects of the MEK inhibitor trametinib, the ERK inhibitor SCH772984 and the CDK4/6 inhibitor ribociclib in human neuroendocrine tumor cell lines BON1, QGP1 and NCI-H727 in vitro. (uni-muenchen.de)
  • Objectives: This study assessed the antitumoral activity of the MEK inhibitor trametinib (TMT212) and the ERK1/2 inhibitor SCH772984, alone and in combination with the CDK4/6 inhibitor ribociclib (LEE011) in human neuroendocrine tumor (NET) cell lines in vitro. (uni-muenchen.de)
  • Mycophenolic-acid (MPA) is a selective, non-competitive inhibitor of Inosine-Monophosphate-Dyhydrogenase (IMPDH) leading to the inhibition of the de-novo synthesis of guanosine-nucleotides. (edu.sy)
  • P27 is a cyclin-dependent kinase inhibitor that negatively regulates cell proliferation. (springeropen.com)
  • Treatment with the novel cyclophilin inhibitor NV651, presented a potent antiproliferative effect in HCC cell lines via cell cycle perturbations arresting cells in the mitotic phase. (lu.se)
  • 1) For example, the down-regulation of miR15s and miR - 16 - 1 in chronic lymphocytic leukaemia, prostate cancer and pituitary tumours, leading to the inhibition of tumour growth and induce cell cycle arrest at the G 1 - phase by target cell cycle regulators ( cyclin D1, cyclin E1, cyclin D3 and CDK6). (ukessays.com)
  • In addition, Mps1 inhibition decreased tumour growth in a xenograft mouse model. (lu.se)
  • Further analyses revealed that simvastatin in C2C12 cells also suppresses PKC and ERK signaling pathways, which are instead activated downstream of CB1 receptor stimulation, thus adding more insight into the mechanism causing CB1 functional inactivation. (bvsalud.org)
  • The inhibition of cell growth was also demonstrated by cell cycle arrest at G2/M phase. (greenmedinfo.com)
  • Natural polyamines, putrescine, spermidine and spermine, play essential roles in the regulation of cell growth and proliferation. (spandidos-publications.com)
  • Transformation was assessed by the standard parameters of morphological alteration, increased hexose uptake, loss of density inhibition, and anchorage-independent growth. (embl.de)
  • Rat GH3 and mouse MMQ pituitary adenoma cells (American Type Tissue Collection, Manassas, VA, USA) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 5% fetal bovine serum, 5% bovine growth serum, and antibiotics in a 37°C humidified incubator at 5% CO 2 . (surgicalneurologyint.com)
  • GH3 cells are a well-characterized model of secretory adenomas that hypersecrete both prolactin (PRL) and growth hormone (GH) and that reflect the biology of human secretory tumors. (surgicalneurologyint.com)
  • HL-60 cells incubated with various amounts (1, 3, 5, 7.5, and 10 micro l/mL) of the extracts for 1-3 days showed dose-dependent, time-dependent growth suppression and decrease in cell viability. (nih.gov)
  • The objective of this study was to investigate the effect of dietary restriction and subsequent compensatory growth on the relative expression of genes involved in volatile fatty acid transport, metabolism and cell proliferation in ruminal epithelial tissue of beef cattle. (biomedcentral.com)
  • We hypothesized that in animals undergoing compensatory growth there would be an up-regulation of the relative expression of genes involved in VFA absorption and metabolism, as well as genes underlying growth and proliferation of the ruminal epithelium. (biomedcentral.com)
  • Cancer chemotherapy is based on an understanding of tumor cell growth and of how drugs affect this growth. (medscape.com)
  • After cells divide, they enter a period of growth (ie, phase G1), followed by DNA synthesis (ie, phase S). The next phase is a premitotic phase (ie, G2), then finally a mitotic cell division (ie, phase M). (medscape.com)
  • Podophyllotoxin derivative antineoplastic agents have cytotoxic effects on cell growth. (medscape.com)
  • As human osteosarcoma cells are a source of additional growth they did not affect osteoinduction. (bvsalud.org)
  • Osteoblasts were obtained from human bone marrow cells even in the absence of growth factors and showed a typical morphology. (bvsalud.org)
  • The normal process of fracture repair begins with an immediate inflammatory response as the innate immune system (macrophages, monocytes, neutrophils, and NK cells) responds with a variety of cytokines that recruit and activate several cell types, including osteoprogenitor mesenchymal stem cells (MSCs), to the site of injury [ 1 , 2 ]. (hindawi.com)
  • However, these immunotherapeutic approaches are hampered by the tumor-promoting nature of myeloid cells, including monocytes, macrophages, and neutrophils. (frontiersin.org)
  • Myeloid innate immune cells, such as granulocytes, monocytes, macrophages and dendritic cells (DCs), play an important role in cancer-cell recognition, initiation of inflammation and antitumor responses ( 2 ). (frontiersin.org)
  • In the present study, I demonstrated that a brief exposure to LeTx caused a continual MEK1 cleavage and prevented TNF-ce production in non-proliferating cells such as human peripheral blood mononuclear cells or mouse primary peritoneal macrophages. (uwo.ca)
  • ERK activation by Cd is not related to decreased proliferation of macrophages but may play a protective role against Cd-induced cytotoxicity. (cdc.gov)
  • The Fidler Lab is focused on understanding how hematopoietic cells, primarily macrophages (Mf), promote atherosclerosis and cardiovascular disease. (ucsf.edu)
  • This CAC is associated with the cell cycle and its regulation is affected by the tumor suppressor proteins. (biomedcentral.com)
  • The recruitment of specific cytosolic proteins to intracellular membranes through binding phosphorylated derivatives of phosphatidylinositol (PtdIns) controls such processes as endocytosis, regulated exocytosis, cytoskeletal organization, and cell signaling. (embl.de)
  • HLA- em DRB1 /em genotypes, high disease activity, high disability scores, and high levels of acute phase proteins [2]. (exposed-skin-care.net)
  • Targeted therapy drugs are used to target specific genes and proteins of cancer cells to precisely identify and attack specific types of cancer cells. (medsci.org)
  • Furthermore, silibinin inhibited the migration and invasion abilities of YD10B and Ca9-22 cells by regulating the expression of proteins involved in the epithelial-mesenchymal transition. (jcancer.org)
  • Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)
  • The effects of silibinin on the migration and invasion of oral cancer cells were evaluated using transwell assays. (jcancer.org)
  • AKAP12 knockdown in benign meningioma cells SF4433 increases proliferation, cell cycle, migration, invasion, and confers an anaplastic profile. (nature.com)
  • HIC inhibited the prostate cancer cells' migration and invasion, revealing its antimetastatic ability. (tuni.fi)
  • Overexpression of SOX9 promoted NPC cell proliferation, migration and invasion. (cancerindex.org)
  • The synthesis, chemical derivatization, and investigation of the inhibitory properties of novel cyclitol derivatives on the phosphatidylinositol 4-kinase enzymes PI4K55 and PI4K230 involved in the phosphatidylinositol cycle are reported. (embl.de)
  • Two mechanisms are involved in the generation of mature piRNAs: a primary synthesis mechanism that is carried out in both somatic and germ cells and a 'ping pong' amplification mechanism that is only formed in the germline [9,20]. (jcimcr.org)
  • The mechanism of action for this antifungal activity is through the decrease of ergosterol synthesis, required for membrane integrity of fungal cells, via inhibition of the lanosterol 14 alpha-demethylase (14DM) catalyst. (ecancer.org)
  • Inhibition of ERK suppressed DNA synthesis and had an additive effect with Cd-inhibited proliferation suggesting that Cd-induced ERK activity is not responsible for the G2/M arrest and subsequent inhibition of cell proliferation. (cdc.gov)
  • 5-FdUMP inhibits thymidylate synthase (key enzyme in DNA synthesis), which leads to accumulation of dUMP, which then gets misincorporated into the DNA in the form of 5-FdUTP resulting in inhibition of DNA synthesis and function with cytotoxic DNA strandbreaks. (medscape.com)
  • P27 is inactivated in cancer cells by various mechanisms including impaired synthesis, accelerated degradation, and mislocalization. (springeropen.com)
  • It acts throughout the entire cell cycle and by direct intercalating into DNA triggers DNA breakage by topoisomerase II, causing subsequent cytocydal activity. (medscape.com)
  • Results: Trametinib and SCH772984, alone and in combination with ribociclib, significantly reduced NET cell viability and arrested NET cells at the G1 phase of the cell cycle in all three cell lines tested. (uni-muenchen.de)
  • Targeting the cell cycle has been proposed as a therapeutic strategy in cancer treatment with special interest in the mitotic phase. (lu.se)
  • We could confirm the increased polyploidy upon Mps1 inhibition in neuroblastoma cell lines and PDX model, leading to mitotic catastrophe activating the caspase-dependent mitochondrial apoptotic pathway. (lu.se)
  • Due to the effect of progesterone, a peak (transient) of mitotic activity occurs in the luteal phase but falls to its nadir in the follicular phase. (medscape.com)
  • Furthermore, pristimerin exhibited a more potent anti‑proliferative activity in CAL‑27 and SCC‑25 cells than the common chemotherapy drugs cisplatin and 5‑fluorouracil. (spandidos-publications.com)
  • Collectively, our results indicate that JIB extract showed anti-tumor effects and synergized with cisplatin against B16/F10 cells, indicating the possibility of JIB extract to be developed as adjuvant therapy for melanoma. (medsci.org)
  • Some agents are specific to phases of the cell cycle, whereas others (eg, alkylating agents, anthracyclines, cisplatin) are not. (medscape.com)
  • The combination of NV651 and cisplatin, a DNA damage reagent, resulted in a synergistic effect in cell viability, and a significant increase on cell death in HCC cell lines. (lu.se)
  • Fifteen micromoles of purvalanol induced a reduction in cell viability by 20 and 35% within 24 and 48 h, respectively. (spandidos-publications.com)
  • [ 59 ] A Phase I/II trial of letrozole and sorafenib was presented at the 2009 American Society of Clinical Oncology Breast Cancer symposium. (medscape.com)
  • Secondary objectives were to determine toxicity, enumerate circulating endothelial cells as an angiogenesis biomarker and analyze the effect of treatment on CYP3A4-metabolized steroids. (medscape.com)
  • Our findings suggest that DIM may ameliorate hyperglycemia and diabetic nephropathy through the inhibition of PKC-α and TGF-β1 signaling. (researchgate.net)
  • In the present study, our aim was to determine the time-dependent, ER-mediated apoptotic and autophagy induction of purvalanol in HCT 116 colon cancer cells. (spandidos-publications.com)
  • Moreover, we determined that during purvalanol-mediated ER stress, autophagic machinery was also activated prior to apoptotic cell death finalization. (spandidos-publications.com)
  • Τheir impact on the apoptotic cell death mechanism requires further elucidation. (spandidos-publications.com)
  • Senescent cells are unable to form the autophagolysosomes and to remove the damaged mitochondria resulting in apoptotic death. (aging-us.com)
  • In addition, trametinib also caused subG1 events and apoptotic PARP cleavage in QGP1 and NCI-H727 cells. (uni-muenchen.de)
  • In the present study, cytotoxic and apoptotic effects of melatonin were investigated on H-ras oncogene transformed rat embryo fibroblast 5RP7 cells. (tubitak.gov.tr)
  • The percentages of early/late apoptotic cells to which melatonin (IC50: 380 μM) was administered were increased 6.2-fold in 5RP7 cells compared to controls for 48 h. (tubitak.gov.tr)
  • In the present study, the anticancer potential of pristimerin was investigated in two oral squamous cell carcinoma (OSCC) cell lines, CAL‑27 and SCC‑25. (spandidos-publications.com)
  • This paper describes how gold nanoparticle nanoconstructs can enhance anticancer effects of lysosomal targeting aptamers in breast cancer cells. (northwestern.edu)
  • Scientists from The Hong Kong University of Science and Technology (HKUST), The University of Hong Kong (HKU) and Institut Curie, France have jointly uncovered a new mechanism of the human MCM2-7 complex in regulating replication initiation, which can be used as a novel and effective anticancer strategy with the potential for selective killing of cancer cells. (sflorg.com)
  • Melatonin may be an anticancer agent against H-ras oncogene activated cancer cells. (tubitak.gov.tr)
  • Because it is very invasive, the prognosis of oral squamous cell carcinoma (OSCC) is poor ( 5 ). (spandidos-publications.com)
  • Besides, 12% of patients have second- or third-degree skin reactions that are sensitive to light, and about 18% of patients develop cutaneous squamous-cell carcinoma and keratoacanthoma or occurred at the same time [ 6 ]. (medsci.org)
  • Although the intended target is the malignant cells of Hodgkin lymphoma, the effects of chemotherapy on normal cells of the body are considerable and account for the adverse effects observed with these agents. (medscape.com)
  • In summary, cyclophilin inhibition in liver fibrosis and HCC could be used as a potential therapeutic strategy, individually or in combination. (lu.se)
  • SET8 depletion causes DNA damage specifically during replication, which induces a Chk1-mediated S-phase checkpoint. (rupress.org)
  • In cells, to initiate DNA replication, MCM2-7 complex must be first assembled into a head-to-head double hexamer (DH) encircling duplex DNA at thousands of places along each chromosome. (sflorg.com)
  • Cell cycle-specific with activity in the S-phase as single agent and has for many years been combined with biochemical modulator leucovorin.Has activity as single agent that inhibits DNA replication and transcription. (medscape.com)
  • This is a heavy metal coordination complex that exerts its cytotoxic effect by platination of DNA, a mechanism analogous to alkylation, leading to interstrand and intrastrand DNA crosslinks and inhibition of DNA replication. (medscape.com)
  • The expression levels of RDM1 mRNA in thyroid cancer cells were measured by quantitative real-time PCR (qRT-PCR). (scienceopen.com)
  • Senescent ERas cells do not develop cytoprotective autophagy upon inhibition of MEK/ERK pathway due to spatial dissociation of lysosomes and autophagosomes in the senescent cells. (aging-us.com)
  • According to recent data, Ras-transformed cells require autophagy to survive and maintain malignant phenotype [ 5 - 10 ]. (aging-us.com)
  • Given that autophagy is initiated upon suppressed mTORC1 activity, a protective autophagy in Ras-transformed cells with a high level of mTORC1 activity appears to be mTORC1-independent [ 20 , 21 ]. (aging-us.com)
  • One of the mechanisms of high basal autophagy activity in cancer Ras-expressing cells might be associated with high PP2A phosphatase activity targeting directly ULK1-Ser757 that could explain apparent contradiction: maintenance of high mTORC1 functions and high autophagic activity simultaneously [ 22 ]. (aging-us.com)
  • Results demonstrated that pristimerin was toxic against the two cell lines, and exhibited inhibitory effects against proliferation. (spandidos-publications.com)
  • Due to the absence of a salvage pathway, proliferating activated t-cells are severely affected by the inhibitory effects of MPA (1-3). (edu.sy)
  • Methods and results: Here, to outline the inhibitory role of HIC on prostate cancer cells, PC-3 and DU145 cell lines were treated with the respective IC50 concentrations, which reduced cell proliferation, adherence properties and spheroid formation. (tuni.fi)
  • Cyclophilin overexpression is a common event in fibrotic tissues playing a key role in different stages of the fibrotic process, including inflammation, hepatocyte death, and activation of the hepatic stellate cells leading to increased collagen production. (lu.se)
  • Overexpression of cyclophilins has been observed in HCC increasing proliferation, metastasis and promoting chemoresistance. (lu.se)
  • Reishi inhibits proliferation and induces programmed cell death in human prostate cancer cells. (greenmedinfo.com)
  • However, the mechanism(s) responsible for the effects of Ganoderma lucidum on cancer cells remain to be elucidated. (greenmedinfo.com)
  • We have previously demonstrated that Ganoderma lucidum down-regulated the expression of NF-kappaB-regulated urokinase plasminogen activator (uPA) and uPA receptor (uPAR), which resulted in suppression of cell migration of highly invasive human breast and prostate cancer cells. (greenmedinfo.com)
  • Thus, Ganoderma lucidum exerts its effect on cancer cells by multiple mechanisms and may have potential therapeutic use for the prevention and treatment of cancer. (greenmedinfo.com)
  • however, the antitumor effects and underlying mechanisms of pristimerin in oral cancer cells have not yet been identified. (spandidos-publications.com)
  • HCT 116 colon cancer cells were exposed to purvalanol, which activated ER stress via upregulation of PERK, IRE1α gene expression, eIF-2α phosphorylation and ATF-6 cleavage at early time-points in the HCT 116 colon cancer cells. (spandidos-publications.com)
  • Cancer cells exploit a variety of protective mechanisms that allow them to acquire selective advantage and proliferate under unfavorable conditions. (aging-us.com)
  • At specific time points related to the doubling time of the cancer cells, we found that accumulation of HER2-HApt-AuNS complexes in lysosomes, lysosomal activity, and lysosomal degradation of HER2 were positively correlated. (northwestern.edu)
  • Western blotting revealed that silibinin downregulated SOD1 and SOD2 and triggered the JNK/c-Jun pathway in oral cancer cells. (jcancer.org)
  • Background: (1-(2-hydroxy-5-nitrophenyl)(4-hydroxyphenyl)methyl)indoline-4-carbonitrile (HIC), an agonist of the P2Y1 receptor (P2Y1R), induces cell death in prostate cancer cells. (tuni.fi)
  • It exerts cytotoxic effect by inhibiting rapid proliferation of cancer cells. (medscape.com)
  • The melatonin treatment resulted in increased caspase-3 activity and reduced the mitochondrial membrane potential in the 5RP7 cells for 48 h. (tubitak.gov.tr)
  • Clinical trials have shown that patients with prostate, lung, and basal cell carcinoma have benefited from treatment with itraconazole, and there are additional reports of activity in leukaemia, ovarian, breast, and pancreatic cancers. (ecancer.org)
  • However, the molecular mechanism behind the inhibition of HIC in prostate cancer remains elusive. (tuni.fi)
  • Sirolimus inhibits T lymphocyte activation and proliferation that occurs in response to antigenic and cytokine (Interleukin IL-2, IL-4, and IL-15) stimulation by a mechanism that is distinct from that of other immunosuppressants. (newlystar-medtech.com)
  • Inhibition of Mps1 led to a potent cytotoxic effect indicating its potential use as a treatment against neuroblastoma. (lu.se)
  • We found Plk1 plays a critical role in maintaining intralysosomal pH by regulating ATP6V1A phosphorylation, and inhibition of Plk1 impaired lysosomal function leading to blockade of autophagic flux. (nature.com)
  • Many breast cancers are dependent for proliferation on estrogens synthesized from androgens (i.e., androstenedione) by aromatase. (aacrjournals.org)
  • We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
  • Compared with traditional antibiotics, AMPs have a broader spectrum of antimicrobial effects, better thermal stability, and less toxicity toward human cells. (researchsquare.com)
  • Recently, the researchers successfully purified the MCM2-7 DH from cultured human cells and determined its structure at 2.59 Å. (sflorg.com)
  • Conclusions: MEK and ERK inhibition causes antiproliferative effects in human NET cell lines in vitro. (uni-muenchen.de)
  • In cell culture experiments, top-type hydrogels demonstrated excessive suppression of human epidermal carcinoma A431 cell proliferation over 5 days due to the initial high concentration of released PTX. (elsevierpure.com)
  • Different volumes of 70% ethanol and water extracts of I'm-Yunity were incubated with cultures of human promyelocytic leukemic HL-60 cells, and compared to nontreated control cells. (nih.gov)
  • NV556 treatment in an in vitro 3D human liver Extracellular Matrix (ECM) cultured with a TGFβ1-activated human hepatic stellate cell line led to decreased collagen production. (lu.se)
  • In human breast tissue specimens, we identified BLV DNA by using nested liquid-phase PCR and DNA sequencing. (cdc.gov)
  • Our finding of BLV in human tissues indicates a risk for the acquisition and proliferation of this virus in humans. (cdc.gov)
  • The finding of antibodies to BLV in humans prompted us to investigate human tissues for evidence of infection with BLV by using liquid-phase PCR (L-PCR), sequencing, in situ PCR, and immunohistochemical (IHC) testing. (cdc.gov)
  • 1987. The effect of electrically generated silver ions on human cells. (cdc.gov)
  • Study design: Osteoinduction in the presence or absence of Saos-2 and/or rhBMP-4 was evaluated in cultured human bone marrow cells. (bvsalud.org)
  • This combination caused a disturbance in the cell cycle and a decrease in the capacity of the cell to repair interstrand crosslinks. (lu.se)
  • Purvalanol is a purine-derived CDKI that binds with a high selectivity and competitively to the ATP binding side of CDK1/2 and leads to G2/M cell cycle arrest ( 4 ). (spandidos-publications.com)
  • This coreceptor then initiates a cell signaling cascade in the muscle that includes the activation of transcription factors in the SMAD family-SMAD2 and SMAD3. (wikipedia.org)
  • PRR on myeloid cells can be triggered by pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs), but also indirectly by secondary mechanisms such as complement activation and circulating antibodies (Abs), resulting in cytolytic and phagocytic effector mechanisms. (frontiersin.org)
  • The successful strategy based on the activation of cell death in Ras-expressing cells is associated with the suppression of kinases involved in Ras pathway. (aging-us.com)
  • Previous studies have found that DIM can improve type 2 diabetes by enhancing glucose uptake through the activation of insulin signaling in 3T3-L1 cells, and by lowering the plasma glucose levels in high-fat-diet-fed obese mice [13, 14]. (researchgate.net)
  • Instead, the increase in ERK activation in cells treated with Cd may reflect a stress response. (cdc.gov)
  • Pharmacological inhibition, genetic silencing or knockout of Plk1 attenuated obstructive nephropathy due to suppressed fibroblast activation mediated by reduced autophagic flux. (nature.com)
  • Thus, targeting fibroblast cell activation and pEMT are critical strategies for attenuating fibrosis. (nature.com)
  • The results supported that inhibition of Plk1 ameliorated kidney fibrosis by suppressing fibroblast activation and partial EMT. (nature.com)
  • 2013). Microglial cell activation plays a major role in the development of this nociceptive sensitization (Wieseler-Frank et al. (niepokorny.org)
  • When the hosts are infected or subject to immune stimulation, AMPs can be quickly synthesized in some tissues and cells. (researchsquare.com)