InducibleINOSInhibitorEndothelial nitric oNeuronalTested the hypothesisReactivityPharmacological inhibitionInhibitorsIsoformsMiceApoptosisSignalingInsulinEnzymeSynthesisSelectiveMoleculeInterleukinPathwayAcuteArginineInducesPhysiologyInhibitoryEnzymesRegulationConcentrationsObesityProductionEffectsPreviouslyLevelsStudyBetaFormationBrainFoundMouse
Inducible15
- Attenuation of inducible nitric oxide synthase gene expression by delta 9-tetrahydrocannabinol is mediated through the inhibition of nuclear factor- kappa B/Rel activation. (aspetjournals.org)
- In the current study, we show that delta 9-THC produces a marked inhibition of inducible nitric oxide synthase (iNOS) transcription and nitric oxide production by the macrophage line RAW 264.7 in response to lipopolysaccharide (LPS). (aspetjournals.org)
- This was abrogated with inhibition of the GR, as well as pan-inhibition of NOS and, specifically, the isoform inducible NOS (iNOS). (brighton.ac.uk)
- All rats were sacrificed at 16 weeks of age, and colon samples were evaluated for ACF, followed by proliferation, apoptosis, and inducible nitric oxide synthase and cyclooxygenase-2, by immunohistochemistry and/or immunoblotting. (nih.gov)
- Furthermore, silibinin significantly decreased AOM-induced inducible nitric oxide synthase- and cyclooxygenase-2-positive cells in colon tissues. (nih.gov)
- Previously, we identified inducible nitric oxide synthase (iNOS) as an essential enzyme for development and reversal of smoke-induced PH and emphysema, and showed that iNOS expression in bone-marrow-derived cells drives pulmonary vascular remodelling, but not parenchymal destruction. (ersjournals.com)
- There are three isoforms of the NO synthesizing enzyme nitric oxide synthase (NOS): neuronal (nNOS), endothelial (eNOS), and inducible nitric oxide synthase (iNOS), each one involved with specific events in the brain. (frontiersin.org)
- The expression of poly(ADP-ribose) (PAR) and inducible nitric oxide (NO) synthase (iNOS) and nitrotyrosine formation were determined immunohistochemically and used as indicators of oxidative and nitrosative stress. (scienceopen.com)
- This overview provides information on the pathophysiology of the inducible nitric oxide synthase/nitric oxide (iNOS/NO) system in the injury to cultured renal tubular epithelia, freshly isolated proximal tubules, and the whole organ after hypoxic or ischemic insult. (scienceopen.com)
- In conclusion, the intricate relations between endothelial and epithelial cells, based in part on the relations between endothelial and inducible nitric oxide synthases, are perturbed in renal ischemia primarily as a result of endothelial dysfunction precipitating epithelial injury. (scienceopen.com)
- Increased inducible nitric oxide synthase expression and oxidation of lung proteins were observed. (cdc.gov)
- Inhibition of inducible nitric oxide synthase with 1400W (1 mg/kg) abrogated the Cl2-induced changes in responsiveness. (cdc.gov)
- Inducible nitric oxide synthase is involved in the induction of changes in responsiveness to methacholine. (cdc.gov)
- This treatment induced a significant increase in the levels of proinflammatory mediators including the inducible Nitric Oxide Synthase (iNOS), interleukin 1 Beta (IL-1β) and Interleukin-12 (IL-12). (lu.se)
- [ 2 ] Some have postulated that salicylates stimulate the expression of inducible nitric oxide synthase (iNOS) because of the findings of iNOS stimulation in African children with fatal malaria, a disease that causes symptoms similar to those of Reye syndrome and is often treated with aspirin. (medscape.com)
INOS3
- This activation of iNOS is attenuated by delta 9-THC through the inhibition of cAMP signaling. (aspetjournals.org)
- Cortisol upregulated the expression of iNOS, a marker previously suggested as an indicator of poor survival in breast cancer.3 Induction of chronic stress in a syngeneic mouse model of breast cancer had no effect on the growth of the primary tumour;however, inhibition of NOS in stressed mice significantly decreased tumour volume compared to stress alone. (brighton.ac.uk)
- Nitric oxide synthase (iNOS) may be a factor regulating the vasodilation of blood vessels, which are relevant to ischemic-hypoxic conditions. (medscimonit.com)
Inhibitor6
- Administration of N ω -nitro-L-arginine (L-NNA), an NO synthase inhibitor (1 g/L, 4 weeks), via drinking water to mice resulted in significant elevations in blood pressure. (elsevierpure.com)
- Asymmetric dimethylarginine (ADMA) is an endogenous nitric oxide (NO) synthesis inhibitor and pro-inflammatory factor. (mdpi.com)
- We examined the effect of selective nitric oxide synthase (NOS) inhibitor N⍵-propyl-L-arginine (NPA) in mice submitted to the contextual fear conditioning and found out that inhibiting NO production with NPA has an antidepressant-like effect on mice. (helsinki.fi)
- An inhibitor of NO synthase blocked L-LTP induced by three-train tetanization and reduced L-LTP induced by four-train tetanization, whereas an inhibitor of PKA was more effective in blocking four-train L-LTP than three-train L-LTP. (jneurosci.org)
- Pretreatment of beta-cells, purified by FACS with IL-1 beta results in a 40% inhibition of glucose-stimulated insulin secretion that is prevented by the nitric oxide synthase inhibitor, NG-monomethyl-L-arginine (NMMA). (jci.org)
- We determined whether administration of a nitric oxide synthase (NOS) inhibitor alters optic nerve head blood flow (ONHBF) regulation during isometric exercise in healthy subjects. (arvojournals.org)
Endothelial nitric o2
- For example, compared with healthy wild-type mice, mice lacking the gene for endothelial nitric oxide synthase (eNOS) develop insulin resistance and hypertension [ 6 ]. (hindawi.com)
- The levels of vascular endothelial (VE)‑cadherin, endothelial nitric oxide synthase (eNOS) and cysteinyl aspartate‑specific protease‑3 (caspase‑3) were measured using western blot analysis. (spandidos-publications.com)
Neuronal2
- The results suggest that remifentanil postconditioning exhibits neuroprotective effects against global cerebral ischemia/reperfusion injury in rats, and its mechanisms might involve inhibition of neuronal apoptosis through the PI3K pathway. (springer.com)
- Effect of inhibition of neuronal nitric oxide synthase and L-arginine supplementation on renal ischaemia-reperfusion injury and the renal nitric oxide system. (tum.de)
Tested the hypothesis2
- This study tested the hypothesis that nitric oxide (NO) synthase inhibition in mice would result in hypertension characterized by increased agonist-induced vasoconstrictor responsiveness and attenuated endothelium-dependent vasodilation. (elsevierpure.com)
- We therefore tested the hypothesis that inhibition of cardiac NO potentiates the positive inotropic response to β-adrenergic stimulation in patients with symptomatic LV failure but not in subjects with normal LV function. (johnshopkins.edu)
Reactivity3
- This study investigated vascular reactivity in response to acetylcholine, in the presence of acute inhibition of nitric oxide synthase, in the carotid artery and aorta of obese C57Bl6/J mice fed on a high-fat diet for 30 weeks, and of control mice. (silverchair.com)
- The aim of the present work was to shed light on the role played by the isoprenoid/cholesterol biosynthetic pathway in the modulation of emotional reactivity and memory consolidation in rodents through the inhibition of the key and rate-limiting enzyme 3-hydroxy 3-methylglutaryl Coenzyme A reductase (HMGR) both in vivo and in vitro with simvastatin. (nature.com)
- The data revealed CB1R inhibition increased basal myogenic tone, but not myogenic reactivity. (bvsalud.org)
Pharmacological inhibition2
- Through pharmacological inhibition of the glucocorticoid receptor (GR), as well as selective and non-selective inhibition of NOS, this research aimed to elucidate a mechanism through which stress may impact breast cancer progression. (brighton.ac.uk)
- These results suggest that genetic down-regulation or pharmacological inhibition of galectin-3 might constitute a novel therapeutic target in PD and other synucleinopathies. (lu.se)
Inhibitors2
- This study indicated that stress may impact tumourigenic progression through the induction of DNA damage, mediated by the release of NO. Inhibition of NOS was able to negate the effects of stress on tumour growth and metastasis, providing an insight into the potential benefits of NOS inhibitors in breast cancer treatment. (brighton.ac.uk)
- A number of studies have shown that inhibitors of NO synthase (NOS), the enzyme for NO production, can prevent the induction of LTP under some experimental conditions. (jneurosci.org)
Isoforms2
- The β-adrenergic agonist dobutamine was infused via a peripheral vein before and during concurrent intracoronary artery infusion of acetylcholine, which activates the agonist-coupled isoforms of NO synthase, and N(G)-monomethyl-L-arginine, which inhibits all isoforms of NO synthase. (johnshopkins.edu)
- Hsp90 plays an important role in nitric oxide (NO) production and the activation of all of the isoforms of nitric oxide synthase (NOS). (eurekaselect.com)
Mice1
- 2020 ) Brain-wide structural and functional disruption in mice with oligodendrocyte-specific deletion is rescued by inhibition of nitric oxide synthase. (neurotree.org)
Apoptosis1
- Ding ZM, Wu B, Zhang WQ et al (2012) Neuroprotective Effects of Ischemic Preconditioning and Postconditioning on Global Brain Ischemia in Rats through the same effect on inhibition of apoptosis. (springer.com)
Signaling1
- Nitric oxide (NO) is thought to be involved in E-LTP, but until now there has been no information about the role of the NO-signaling pathway in L-LTP. (jneurosci.org)
Insulin5
- Nitric oxide synthase inhibition decreases output of hepatic insulin sensitizing substance (HISS), which is reversed by SIN-1 but not by nitroprusside. (unl.pt)
- Insulin resistance and obesity are characterized by low nitric oxide (NO) bioavailability. (hindawi.com)
- In rats, disruption of NO generation via nitric oxide synthase inhibition attenuates insulin mediated glucose uptake [ 7 ]. (hindawi.com)
- Nitric oxide has recently been implicated as the effector molecule that mediates IL-1 beta-induced inhibition of glucose-stimulated insulin secretion and beta-cell specific destruction. (jci.org)
- The purpose of this investigation was to determine if the beta-cell, which is selectively destroyed during insulin-dependent diabetes mellitus, is both a source of IL-1 beta-induced nitric oxide production and also a site of action of this free radical. (jci.org)
Enzyme2
- Diagram showing the synthetic pathway responsible for nitric oxide (NO) production from arginine and O 2 and catalyzed by the enzyme nitric oxide synthase (NOS). (frontiersin.org)
- Nitric oxide (NO) is formed from arginine by the enzyme nitric oxide synthase (NOS). (medscape.com)
Synthesis2
- Nitric oxide synthesis. (frontiersin.org)
- The number of cells per field, the AP activity, the collagen and mineralized matrix synthesis were higher in AT-MSC en differentiation, in relation to BM-MSC under the same conditions in all evaluated times. (scielo.br)
Selective1
- The results of the present study demonstrate that selective inhibition of nNOS has no effect on renal injury, indicating that nNOS does not play a central role in the pathophysiology of renal I/R. In addition, although L-arginine has no effect on renal I/R injury in the model used in the present study, its administration increases the mRNA expression of NOS isoforms. (tum.de)
Molecule2
- Nitric oxide (NO) is a versatile messenger molecule first associated with endothelial relaxing effects. (frontiersin.org)
- Nitric oxide (NO) is an important signalling molecule in the brain. (helsinki.fi)
Interleukin1
- Interleukin 1 beta induces the formation of nitric oxide by beta-cells purified from rodent islets of Langerhans. (jci.org)
Pathway2
- Conflicting results have been reported regarding the role of the nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) pathway in the hippocampus on anxiety modulation. (nih.gov)
- We have analysed the mechanism by which palmitoylation permits the progression of nitric oxide synthase 2 (NOS2) along the ER-Golgi-TGN pathway. (biologists.com)
Acute2
- Nitric oxide in acute renal failure: NOS versus NOS. (scienceopen.com)
- A reduction in exhaled nitric oxide (NO) levels was first observed in smokers in the early 1990s 1 , 2 , and this effect was found after both acute and chronic exposure to smoking 3 . (ersjournals.com)
Arginine2
- The role of nitric oxide synthases (NOS) and the nitric oxide (NO) substrate l-arginine in renal ischaemia-reperfusion (I/R) has been studied extensively. (tum.de)
- Therefore, the detrimental effect of ADMA might be inhibited by increasing the concentration of arginine or the concentration of its precursors, citrulline and glutamine, which would increase the arginine/ADMA ratio and thereby might reverse the competitive inhibition of NOS by ADMA. (medscape.com)
Induces3
- Our results show that HMGR inhibition by simvastatin induces anxiogenic-like effects in the social interaction but not in the elevated plus-maze test, and improves memory consolidation in the inhibitory avoidance task. (nature.com)
- IL-1 beta induces the formation of nitric oxide by purified beta-cells as evidenced by the accumulation of cGMP, which is blocked by NMMA. (jci.org)
- IL-1 beta induces the formation of an iron-dinitrosyl complex by Rin-m5F cells indicating that nitric oxide mediates the destruction of iron-sulfur clusters of iron containing enzymes. (jci.org)
Physiology1
- Nitric oxide (NO) is a prominent compound in the heart and its vasculature and plays an intriguing role in the physiology of this organ. (medscape.com)
Inhibitory1
- Intramural inhibitory nerves act by releasing nitric oxide ( NO ) and vasoactive intestinal peptide, whereas the excitatory nerves release acetylcholine and substance P. (nature.com)
Enzymes1
- These results demonstrate that the islet beta-cell is a source of IL-1 beta-induced nitric oxide production, and that beta-cell mitochondrial iron-sulfur containing enzymes are one site of action of nitric oxide. (jci.org)
Regulation1
- Nitric oxide has an important role in basal ONHBF regulation, but seems not to be involved in the autoregulatory response during an increase in OPP induced by isometric exercise. (arvojournals.org)
Concentrations2
- Using extended nitric oxide analysis, it was possible to attribute the reduction in exhaled nitric oxide levels seen in ex- and current smokers to a lower total airway nitric oxide flux in ex-smokers and reduced airway and alveolar nitric oxide concentrations in current smokers. (ersjournals.com)
- The association between snus (oral tobacco) use and reduced nitric oxide concentrations in the airways and increased nitric oxide transfer from the airways warrants further studies. (ersjournals.com)
Obesity1
- Thus obesity increases endothelium-dependent vasoconstriction in the absence of endothelial nitric oxide. (silverchair.com)
Production2
- In animal models, inhibition of NO production triggers behavioural effects similarly to those induced by antidepressant drugs. (helsinki.fi)
- The possible mechanisms by which exhaled NO levels are reduced in smoking subjects are a potential negative feedback mechanism of the NO from the cigarette smoke, which could lead to downregulation of NO synthase (NOS) in the lungs 8 , 9 , an inadequate supply of cofactors necessary for NO production, such as tetrahydrobiopterin 10 , and an increase in the breakdown of NO 11 , 12 . (ersjournals.com)
Effects3
- delta 9-Tetrahydrocannabinol (delta 9-THC) a prototypic compound belonging to the family of agents known as cannabinoids, produces a wide variety of biological effects, including inhibition of immune function. (aspetjournals.org)
- Primarily, these included the effects of NOS inhibition on haemodynamics, because L-NAME has been used previously in a clinical setting to increase blood pressure. (brighton.ac.uk)
- The few studies investigating the effects of smoking on flow-independent NO exchange parameters indicate that smoking is related to a lower maximum total airway nitric oxide flux ( J ' aw,NO ) 7 , 14 and to a lower mean airway tissue nitric oxide concentration ( C aw,NO ) 15 . (ersjournals.com)
Previously1
- Background - We previously showed that cardiac nitric oxide (NO) inhibits the positive inotropic response to β-adrenergic stimulation in humans with left ventricular (LV) dysfunction. (johnshopkins.edu)
Levels1
- It is a well-known fact that smoking is associated with a reduction in exhaled nitric oxide (NO) levels. (ersjournals.com)
Study1
- The aim of this study was to investigate whether the inhibition of human sperm cNOS could affect sperm-oocyte fusion and sperm binding to the zona pellucida (ZP). (bioone.org)
Beta2
- Evidence for the beta-cell as a source and site of action of nitric oxide. (jci.org)
- This is further demonstrated by IL-1 beta-induced inhibition of glucose oxidation by purified beta-cells, mitochondrial aconitase activity of dispersed islet cells, and mitochondrial aconitase activity of Rin-m5F cells, all of which are prevented by NMMA. (jci.org)
Formation1
- Genetic or pharmacologic inhibition of NOS1 reduced the growth of CXCL14-expressing fibroblasts along with their ability to promote tumor formation following coinjection with prostate or breast cancer cells. (lu.se)
Brain1
- Nitric oxide (NO) is one of the most intriguing molecules to be found in the brain. (frontiersin.org)
Found1
- We found that the nitric oxide synthase NOS1 is upregulated in CXCL14-expressing CAF and in fibroblasts stimulated with CXCL14. (lu.se)
Mouse1
- There is recent evidence that mouse and human spermatozoa contain constitutive nitric oxide synthase (cNOS) and can synthesize nitric oxide. (bioone.org)