• Interestingly, AVM appeared to be more sensitive to imatinib‑resistant (T315I, Y253H, and E279K) than wild‑type BCR/ABL cells, indicating its potential to overcome imatinib‑resistant severe issues in CML. (spandidos-publications.com)
  • By colorimetric MTT assay, it was found that contrast media (0-3500 μM) had no effect on both K562 and K562/adr cell viabilities, but in co-treatment with daunorubicin (DNR), diatrizoate decreased cell viability in K562/adr cells by decreasing IC so of DNR from 610.7 ±74.5 nM to 360±108.9 nM. (thescipub.com)
  • Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
  • The anti-proliferative effects of the bis-4-hydroxycoumarin-type compound on human K-562 (chronic myeloid leukaemia) and JURKAT (acute T-cell leukaemia) cell lines using trypan blue staining, as well as its involvement in nuclear factor-kappa B (NF-kappa B) regulation analyzed by luciferase reporter gene assay, gene expression analysis and western blots were analysed. (ua.pt)
  • MTT assay was used to evaluate cell growth inhibition. (edu.hk)
  • Methods: The effect of berbamine on the KU812 cell growth was determined by methyl thiazolyl tetrazolium (MTT) assay. (zju.edu.cn)
  • The B16F10 cell line viability after treatment with increasing concentrations of different fractions of the plant (5-60 µg/mL) was measured using MTT assay. (ac.ir)
  • Second, SB203580 inhibited the inhibitory effect of activin A on the colony-forming activity of K562 cells using the methylcellulose colony assay, indicating that activin A inhibits K562 colony formation by activating p38 MAPK. (tmu.edu.tw)
  • To validate the ER tension model and assess cell survival pursuing thapsigargin challenge, proteins markers for ER tension and apoptosis aswell as cell success had been evaluated using American blot evaluation and MTT assay. (health-e-nc.org)
  • The main target of this study was to address the effects of ERK5 inhibition on the maintenance of hypoxiaselected LSC of CML by the Culture-Repopulating Ability (CRA) assay. (unifi.it)
  • At a level of 10 microg mL(-1) DEHP a significant anti-leukaemic effect was observed for all three cell lines, as measured by clonogenic assay. (tripod.com)
  • Potentiation of vincristine-induced growth inhibition by these MFs was also tested in K562/ADM cells by means of WST-1 [2-(4-iodophenyl)-3-(4- nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] assay. (elsevierpure.com)
  • Furthermore, there was a significant correlation between the potencies for increasing [ 3 H]vincristine uptake and for growth inhibition assessed by WST-1 assay. (elsevierpure.com)
  • The inhibitory effects of bufalin on cell proliferation were determined by MTT (Methyl thiazolyltetrazolium) assay. (biomedcentral.com)
  • First, we show herein that activin A induced the expression and activated the promoters of α-globin and ζ-globin in K562 cells, confirming that activin A induces erythroid differentiation in K562 cells. (tmu.edu.tw)
  • In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. (molcells.org)
  • The up-regulation of miR-199b-5p during erythroid differentiation was dependent on the binding of GATA-1 and NF-E2 to its gene locus, which activated its transcription and maintained its high expression level in mature erythroid cells. (molcells.org)
  • Exosomal miR-486 regulates hypoxia-induced erythroid differentiation of erythro-leukemia cells through targeting Sirt1[J]. Exp Cell Res, 2017, 351:74-81. (magtechjournal.com)
  • Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)
  • Bruceine D exhibited potent cytotoxicity to K562 cells with IC50 of 6.37 ± 0.39 μM. (edu.hk)
  • Importantly, Spautin-1 enhances IM-induced cytotoxicity in CML cell line K562, decreasing the IC 50 from 1 to 0.5 μM. (cancer-research-network.com)
  • Extracts of Aloe vera Linne have been found to exhibit cytotoxicity against human tumour cell lines. (tripod.com)
  • The cytotoxicity mechanism consists of the induction of apoptosis, whereas the selectivity against neuroectodermal tumor cells is founded on a specific energy-dependent pathway of drug incorporation. (tripod.com)
  • We conclude that activin A can regulate the same pathway via p38 MAPK to coordinate cell proliferation and differentiation of K562 cells. (tmu.edu.tw)
  • AVM induced the release of cleaved PARP and cleaved caspase‑3 caused apoptosis and inhibited the viability of these cells. (spandidos-publications.com)
  • In previous research, the authors demonstrated that the methanol extract of A. vulgaris (AVM) has the ability to inhibit the viability of CML cells ( 10 ). (spandidos-publications.com)
  • Interestingly, it exerted mainly cytostatic effects at low doses on both cell lines tested, whereas it decreased JURKAT cell viability starting at 50 mu M from 24 h of treatment. (ua.pt)
  • Importantly, it did not affect the viability of peripheral blood mononuclear cells (PBMCs) from healthy donors, even at concentrations above 100 mu M. (C) 2014 Elsevier Ltd. All rights reserved. (ua.pt)
  • Knockdown of circ_SIRT1 regulated K562/R cells viability, invasion and apoptosis. (bvsalud.org)
  • This compound inhibited TNF alpha-induced NF-kappa B activation in K-562 (IC50 17.5 mu M) and JURKAT (IC50 19.0 mu M) cell lines, after 8 h of incubation. (ua.pt)
  • Besides, the inhibition of circ_SIRT1 attenuated autophagy level and reduced IC50 to Imatinib of K562/R cells. (bvsalud.org)
  • The IC50 of phenytoin sodium and doxorubicin in K562/Dox cells was considerably greater than that in wild-type K562 cells indicating the medication level of resistance of K562/Dox cells. (cancerrealitycheck.com)
  • Through the preventing of p38 activity in the current presence of SB202190 cellular number was considerably reduced following the phenytoin sodium and doxorubicin treatment as well as the IC50 of phenytoin sodium and doxorubicin was Isorhynchophylline reduced in K562/Dox cells. (cancerrealitycheck.com)
  • After preventing p38 with 10 μM SB202190 in K562/Dox cells we noticed the fact that IC50 of phenytoin was considerably reduced from 2186.33±214.70 to 949.83±131.31 μg/mL with an RI of 2.30 much like that of the verapamil control (2.56). (cancerrealitycheck.com)
  • The IC50 of doxorubicin was also low in cells treated with 10 μM SB202190 than in neglected K562/Dox cells (4.33±0.50 μg/mL and 0.40±0.09 μg/mL respectively) with an RI of 10.83 much like that of verapamil (12.37). (cancerrealitycheck.com)
  • The focus … Desk 1 IC50 of doxorubicin and phenytoin in K562 and Isorhynchophylline K562/Dox cells. (cancerrealitycheck.com)
  • Objective: The cytotoxic effect of berbamine on chronic myeloid leukemia (CML) cell line KU812 was evaluated, and the mechanisms of its action were explored. (zju.edu.cn)
  • The inhibition of P-gp and other ABC transporters can increase because the tumours have metastasized and/or have become resistant the intracellular concentration of cytotoxic drugs. (gotomydoctor.com)
  • In LC1, this inhibition resulted in a significant increase of the percentage of cells in G0/G1 and in a modest reduction of apoptosis, pointing to a cytostatic, rather than cytotoxic, effect of ERK5 inhibition. (unifi.it)
  • In the last years, adoptive transfer of natural killer (NK) cells came into the focus of translational medicine, because of their high cytotoxic potential against transformed malignant cells. (frontiersin.org)
  • Hence, we established a two-phase expansion protocol with IL-15 to induce an early NK cell expansion, followed by short exposure to IL-21 that boosted the cytotoxic activity of NK cells against RMS cells. (frontiersin.org)
  • however, the study of ultrafine Pt NCs is at the beginning stage and there are still plenty of challenges like synthesis of near-infrared (NIR) fluorescent Pt NCs, the explicit signal pathway of cell apoptosis, and attempt in diverse biological applications that need to be urgently tackled in future. (hindawi.com)
  • It led to loss of Δψm, release of cytochrome c, activation of caspases-9, -3 and cleavage of PARP, which suggested that bruceine D induced apoptosis of K562 cells through mitochondrial pathway. (edu.hk)
  • The effects of the inhibition of the MEK5>ERK5 pathway were then determined. (unifi.it)
  • The purpose of the research is to investigate the roles of Jak-STAT3 signaling pathway in bufalin-induced apoptosis in colon cancer SW620 cells. (biomedcentral.com)
  • Activation of caspase-3, up-regulation of Bax, down-regulation of livin and Bcl-2, as well as inhibition of Jak-stat3 signaling pathway might be the important mechanisms for the bufalin-induced apoptosis. (biomedcentral.com)
  • Berbamine inhibits proliferation and induces apoptosis of KU812 cells by increasing Smad3 activity[J]. Journal of Zhejiang University Science B, 2011, 12(7): 568-574. (zju.edu.cn)
  • Huang, HM , Chiou, HY & Chang, JL 2006, ' Activin A induces erythroid gene expressions and inhibits mitogenic cytokine-mediated K562 colony formation by activating p38 MAPK ', Journal of Cellular Biochemistry , vol. 98, no. 4, pp. 789-797. (tmu.edu.tw)
  • Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
  • Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)
  • Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)
  • Chronic myeloid leukemia (CML), an acquired malignant myeloproliferative disorder of hematopoietic stem cells, is one of the three most common forms of leukemia. (edu.hk)
  • Treatment of drug-resistant Caco-2 cells with the most active C. jambhiri and C. pyriformis compounds increased their sensitivity to doxorubicin and completely reversed doxorubicin resistance, which agrees with a decreased P- gp activity. (gotomydoctor.com)
  • Compounds (C1-8 and doxorubicin) were placed to 96-well culture plate in triplicate order at doses of 1, 10, 100, 1000 μM and then K562 cells were seeded into the wells at the dose of 105/ml. (ijpsonline.com)
  • HPLC demonstrated the fact that intracellular degrees of phenytoin sodium and doxorubicin had been considerably low in K562/Dox cells than those in K562 cells. (cancerrealitycheck.com)
  • The intracellular degrees of phenytoin and doxorubicin had been considerably low in K562/Dox cells than those in K562 cells (Body 4A B) additional confirming the medication level of resistance of K562/Dox cells. (cancerrealitycheck.com)
  • The loss of the intracellular degree of phenytoin and doxorubicin in K562/Dox cells was considerably abolished in the current presence of SB202190 (Body 4). (cancerrealitycheck.com)
  • K562/Dox cells had been pretreated with SB202190 for one hour and treated with phenytoin (A) or doxorubicin (B) for 48 hours in the current presence of SB202190. (cancerrealitycheck.com)
  • Verapamil was utilized Isorhynchophylline as a confident … Body 4 Aftereffect of SB202190 Isorhynchophylline on focus of doxorubicin and phenytoin in K562/Dox cells. (cancerrealitycheck.com)
  • Given the important role of both cytokine-mediated inflammatory microenvironment and cytolytic NK cell activities in immune-oncology therapies, our findings present a compelling argument for further clinical investigation. (frontiersin.org)
  • The inhibition of p38 MAPK reverted the inhibitory effect of activin A on mitogenic cytokine-mediated K562 colony formation. (tmu.edu.tw)
  • Also zinc nanoparticles significantly (p=0.021) inhibit K562 cell line. (ac.ir)
  • Based on this finding it should be concluded that combination of zinc nanoparticles with Saccharomyces cell walls could inhibit the growth of K562 cell line in vitro . (ac.ir)
  • This is supported by pharmacological targeting where the first-generation pan-BET bromodomain inhibitor JQ1(+) displays anti-inflammatory effects and inhibit tumor cell eradication, while the novel bivalent BET bromodomain inhibitor AZD5153, which shows differential activity towards BET family members, does not. (frontiersin.org)
  • These mice are devoid of endogenous natural killer and T-cell activity and were used to determine whether adoptively transferred expanded natural killer cells could inhibit myeloma growth and myeloma-associated bone destruction. (haematologica.org)
  • In previous research, the authors demonstrated that the methanol extract of Artemisia vulgaris (AVM) has the ability to inhibit chronic myeloid leukemia (CML) cell proliferation. (spandidos-publications.com)
  • The compound does not inhibit the proliferation of normal fibroblasts nor that of hemopoietic progenitor cells. (tripod.com)
  • Conclusions These studies provide the rationale for testing expanded natural killer cells in humans. (haematologica.org)
  • In addition, mitogenic cytokines SCF, IL-3, and GM-CSF induced colony formation of K562 cells that could be inhibited by PD98059 or enhanced by SB203580, respectively, indicating that these mitogenic cytokines induce K562 colony formation by activating ERK and inactivating p38 MAPK. (tmu.edu.tw)
  • Chronic myeloid leukemia is a common cancer in human, so the goal of this study was the use of natural compound such as cell wall obtained from Saccharomyces cerevisiae (S. cerevisiae) and Saccharomyces boulardi (S. boulardi) and zinc nanoparticles on the growth inhibition of K562 cell line. (ac.ir)
  • Anti - cancer property of different concentrations of the yeasts' cell wall with zinc nanoparticles were assayed by MTT and electrophoresis methods. (ac.ir)
  • But these anti-cancer properties would warrant further study on the clinical application of yeast cell wall. (ac.ir)
  • The result on this occasion was the initiation of apoptosis (programmed cell death) in cancer cells, which shows that curcumin can be an anti-cancer agent. (antiaging-systems.com)
  • Cancer cells frequently display intrinsic or acquired resistance to chemically diverse anticancer drugs, limiting therapeutic success. (nature.com)
  • Multidrug resistance was an obstacle in cancer chemotherapy because the cells decreased their intracellular drug accumulation by energy-dependent compounds efflux pumps such as P-glycoprotein (P-gp). (thescipub.com)
  • Similarly, single cell methods are vital in stem cell research and cancer biology, where isolated populations of primary cells are heterogeneous due to limitations in purification protocols, and it is often a minority cell population that is the most relevant. (justia.com)
  • Effects of sinusoidal magnetic field observed on cell proliferation, ion concentration, and osmolarity in two human cancer cell lines med. (emf-portal.org)
  • Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
  • The Extracellular signal-Regulated Kinase 5 (ERK5) is a member of the mitogen-activated protein kinase family involved in the control of cell survival and proliferation and in the pathogenesis of different types of cancer, including CML. (unifi.it)
  • Bufalin not only inhibited the growth of colon cancer SW620 cells, but also induced apoptosis of SW620 cells. (biomedcentral.com)
  • PGP is really a transmembrane glycoprotein working as a medication transport that positively pumps out a number of anti-cancer agencies as well as other hydrophobic substances through the cells [7 20 hence reducing intracellular medication concentrations and resulting in medication resistance [10]. (cancerrealitycheck.com)
  • The K562 and KCL-22 human stabilized CML cell lines, where ERK5 is constitutively activated, were used both for all the experiments. (unifi.it)
  • Inhibition of DNA-dependent protein kinase catalytic subunit by small molecule inhibitor NU7026 sensitizes human leukemic K562 cells to benzene metabolite-induced apoptosis. (cdc.gov)
  • Mice transduced with both ASXL1 and RUNX1 mutations enhanced inhibitor of DNA binding 1 (ID1) expression in the spleen, liver, and bone marrow cells. (biomedcentral.com)
  • PGP-overexpressing K562/Dox cells allow us to study the effect of p38 inhibitor on drug resistance. (cancerrealitycheck.com)
  • in North America reported using Vero cell vaccines, which are not licensed in either the United States or Canada, PI3K inhibitor but it is unclear if these vaccines were actually used in these clinics or whether the clinician erroneously reported their use. (c-kitinhibitors.com)
  • Induction of apoptosis in human leukaemic cell lines K562, HL60 and U937 by diethylhexylphthalate isolated from Aloe vera Linne. (tripod.com)
  • We investigated the effect of diethylhexylphthalate (DEHP) from Aloe vera Linne on the apoptosis of human leukaemic cell lines K562, HL60 and U937 to examine its pharmacological activity. (tripod.com)
  • At 100 microg mL(-1) DEHP, K562, HL60 and U937 leukaemic cell lines showed growth inhibition of 95, 97 and 95%, respectively. (tripod.com)
  • DEHP exhibited an inhibitory activity of 74, 83 and 81%, respectively, in K562, HL60 and U937 cell lines at a concentration of 10 microg mL(-1). (tripod.com)
  • At a concentration of 1 microg mL(-1), DEHP exerted an inhibitory activity of 50, 51 and 52%, respectively, in K562, HL60 and U937. (tripod.com)
  • Cell lines and cell tradition The K562 (CML), KU812 (CML), MEG-01 (CML), MV4-11 (AML), MOLM14 (AML), U937 (AML), REC-1 (Human being B-cell lymphoma cell), HL-60 (Human being promyelocytic leukemia cells), MEC-1(CLL), CHL. (molecularcircuit.com)
  • We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)
  • Inevitably, there are fundamental and applied questions, such as those relating to transcriptional control of stem cell differentiation, intrinsic noise in gene expression, and the origins of disease, that may only be addressed at the single cell level. (justia.com)
  • For example, single cell analysis allows for the direct measurement of gene expression kinetics, or for the unambiguous identification of co-regulated genes, even in the presence of de-synchronization and heterogeneity that could obscure population-averaged measurements. (justia.com)
  • Thus, many of the basic functionalities for microfluidic single cell gene expression analysis have been demonstrated in isolation, including cell manipulation and trapping (9, 10), RNA purification and cDNA synthesis (11-13), and microfluidic qPCR (14) following off-chip cell isolation cDNA synthesis and preamplification. (justia.com)
  • Interestingly, Spautin-1 enhances imatinib mesylate (IM)-induced CML cell apoptosis by reducing the expression of the anti-apoptotic proteins Mcl-1 and Bcl-2. (cancer-research-network.com)
  • Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. (molcells.org)
  • Peripheral blood mononuclear cell (PBMC) miR-486, Sirt1 and GATA-1 expression were measured by RT-qPCR, and the concen- tration of plasma Sirt1 was measured by ELISA. (magtechjournal.com)
  • In addition, RT-qPCR was used for the miR-486, Sirt1 or expression in K562 cells after the IH treatment, miR-486 over-expression or miR-486 inhibition. (magtechjournal.com)
  • GATA-1 mRNA expression was checked by RT-qPCR in Sirt1 inhibited K562 cells. (magtechjournal.com)
  • 4)IH promoted the expression of miR-486( P <0.01) and inhibited Sirt1 expression in K562 cells( P <0.01). (magtechjournal.com)
  • RT-qPCR and Western blot showed that over-expression of miR-486 decreased Sirt1 expression( P <0.01), whereas miR-486 inhibition increased the expression of Sirt1 ( P <0.001). (magtechjournal.com)
  • The GATA-1 expression was increased after the inhibition of Sirt1 ( P <0.01). (magtechjournal.com)
  • To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
  • Co-expression of two mutant genes increased myeloid stem cells in animal model, suggesting that cooperation of RUNX1 and ASXL1 mutations played a critical role in leukemia transformation. (biomedcentral.com)
  • The expression of RUNX1 mutant in ASXL1 -mutated myeloid cells augmented proliferation, blocked differentiation, and increased self-renewal activity. (biomedcentral.com)
  • Moreover, the RUNX1 mutant protein was more stable than WT and increased HIF1-α and its target ID1 gene expression in ASXL1 mutant cells. (biomedcentral.com)
  • Biedler, J. L. & Riehm, H. Cellular resistance to actinomycin D in Chinese hamster cells in vitro: cross-resistance, radioautographic, and cytogenetic studies. (nature.com)
  • Cell-based and in vitro assays show that Retro-2 blocks delivery of newly-synthesized TA-proteins to the ER-targeting factor ASNA1 (TRC40). (elifesciences.org)
  • an ATP-binding ), coumarins () and terpenoids cassette (ABC) transporter (150-170 kDa), which pumps out lipo- have been reported as philic agents from cells that have entered them by free diffusion agents for overcoming multidrug resistance, and could be used aloneat very low concentrations or in combination to reverse multidrugresistance in vitro. (gotomydoctor.com)
  • Autophagy refers to the natural and conservative degradation of cells through lysosome dependent regulation mechanism. (cancer-research-network.com)
  • The mechanism of spautin-1 acting on acute pancreatitis is relevant to impaired autophagy inhibition. (cancer-research-network.com)
  • The results showed that S. boulardi cell wall significantly (p=0.029) inhibits the growth of K562 cell line compared to S. cerevisiae . (ac.ir)
  • Moreover, NK cells are poised to release cytokines such as IFN- γ , TNF-α and growth factors that can initiate inflammatory responses mediated by both the innate and the adaptive arm of the immune system. (frontiersin.org)
  • Adoptive transfer of expanded natural killer cells inhibited the growth of established OPM2 and high-risk primary myeloma tumors grown in the murine model. (haematologica.org)
  • The transferred, expanded natural killer cells proliferated in vivo in an interleukin-2 dose-dependent fashion, persisted up to 4 weeks, were readily detectable in the human bone, inhibited myeloma growth and protected bone from myeloma-induced osteolysis. (haematologica.org)
  • Growth inhibition and apoptosis induction of Scutellaria luteo-coerulea Bornm. (ac.ir)
  • A role for transcriptional repression of p21CIP1 by c-Myc in overcoming transforming growth factor β-induced cell-cycle arrest. (zju.edu.cn)
  • Growth inhibition was specifically exerted by DEHP against three leukaemic cell lines at concentrations below 100 microg mL(-1). (tripod.com)
  • In a normal cell line, MDBK, DEHP exerted 30% growth inhibition at a concentration of 100 microg mL(-1), and showed no inhibitory activity at concentrations below 50 microg mL(-1). (tripod.com)
  • Furthermore, activin A reduced the induction effect of these mitogenic cytokines on K562 colony formation in a dose-dependent manner. (tmu.edu.tw)
  • Besides surgery, radiotherapy and chemotherapy, immune activation by direct application of cytokines, antibodies or adoptive cell therapy are promising approaches. (frontiersin.org)
  • In this study, we addressed feeder cell-free expansion methods using common γ-chain cytokines, especially IL-15 and IL-21. (frontiersin.org)
  • In vivo development and survival of NK cells require cytokines ( 6 - 8 ). (frontiersin.org)
  • In this context, cytokines have been shown to activate NK cells potently during ex vivo expansion ( 9 - 12 ). (frontiersin.org)
  • This study observed some iodinated radiographic contrast media, diatrizoate, iopromide and iotrolan affected the cellular energetic state and the kinetics of P-gp in drug-sensitive K562 and drug resistant K562/adr cell lines using spectrophotometer and spectrofluorometer. (thescipub.com)
  • 468.82 μg/mL and 4.33±0.50 0.32±0.05 μg/mL respectively) (Table 1) indicating that K562/Dox cells had been drug-resistant. (cancerrealitycheck.com)
  • Dano, K. Active outward transport of daunomycin in resistant Ehrlich ascites tumor cells. (nature.com)
  • New therapeutic concepts in anti-tumor therapy aim to modulate the patient's immune system to increase its aggressiveness or targeted effects toward tumor cells. (frontiersin.org)
  • It's been proven that long-term publicity of tumor cells for some varieties of chemotherapy medications causes level of resistance [21]. (cancerrealitycheck.com)
  • In particular, NK cells mediate their cytolytic function through the engagement of activating receptors, such as NKG2D, DNAM-1, NKp30, NKp46, and NKp44 ( 3 , 4 ), or following pro-inflammatory cytokine stimulation ( 5 ). (frontiersin.org)
  • Leukemia cells in patients are intrinsically resistant to standard chemotherapy, and resistance development in leukemia cells toward drugs results in treatment failure[ 4 , 5 ]. (ijpsonline.com)
  • Evidences obtained in our laboratory indicated that the leukaemia stem cell (LSC) potential of chronic myeloid leukaemia (CML) cell populations is resistant to, and selected in, severe hypoxia (0.1-1% oxygen). (unifi.it)
  • The maintenance of leukaemia stem cells of CML is impaired by ERK5 inhibition, which therefore emerges as a potential novel strategy for CML therapy. (unifi.it)
  • This approach was based on the assumption that the deregulation of a protein was causally linked to a disease state, and the pharmacological intervention through inhibition of the deregulated target was able to restore normal cell function. (biomedcentral.com)
  • These results indicate that DEHP isolated from Aloe vera Linne has a potent antileukaemic effect, and thus represents a new type of pharmacological activity with respect to human leukaemic cells. (tripod.com)
  • In a proof of concept in vivo study, we also observed a therapeutic effect of adoptively transferred IL-15 expanded and IL-21 boosted NK cells in combination with image guided high precision radiation therapy using a luciferase-transduced RMS xenograft model. (frontiersin.org)
  • In the CML cell K562 inoculated xenograft mouse model, dental administration of 100 mg/kg/d of CHMFL-074 accomplished a tumor development inhibition (TGI) of 65% without exhibiting obvious toxicity. (molecularcircuit.com)
  • D) American blot evaluation with antibodies particular towards the indicated protein from tumor lysates ready through the K562 xenograft mice upon the conclusion of the indicated remedies. (molecularcircuit.com)
  • berbamine suppresses KU812 cell proliferation through induction of cell cycle arrest in G 1 and apoptosis . (zju.edu.cn)
  • 3.3 Aftereffect of ER strain and ALDH2 on myocardial histology, ER strain and cell survival To measure the impact 287714-41-4 manufacture of ALDH2 transgene on myocardial histology subsequent ER strain induction, cardiomyocyte cross-sectional area and interstitial fibrosis had been examined. (health-e-nc.org)
  • However, direct injection of IL-2 has been shown to be accompanied by severe side effects, such as vascular leak syndrome, activation-induced cell death, and strong induction of regulatory CD4 pos T cells, which did not occur after IL-15 administration ( 15 , 16 ). (frontiersin.org)
  • Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
  • berbamine induced G 1 arrest as well as apoptosis in KU812 cells . (zju.edu.cn)
  • As a result, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 (human wild‑type) or TCCY‑T315I (human imatinib‑resistant) and the Ba/F3‑(T315I/E279K/Y253H) (mouse BCR/ABL point mutation‑transfected cells). (spandidos-publications.com)
  • Thus, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 [human wild-type (WT)] or TCCY-T315I [human imatinib-resistant (IR)] and the Ba/F3-(T315I/E279K/Y253H) (mouse BCR/ABL point mutation-tranfected cells). (spandidos-publications.com)
  • on human chronic myeloid leukemia K562 cells. (edu.hk)
  • In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)
  • This study examines the anti-tumour effects of di(2-ethylhexyl)phthalate (DEHP) isolated from Aloe vera Linne, in human and animal cell lines. (tripod.com)
  • Vero cell vaccines were reported more commonly from respondents in Eastern Europe, Asia, and Africa, in contrast to clinics in North America and Western Europe, which primarily reported using human diploid cell and purified chick embryo cell vaccines. (c-kitinhibitors.com)
  • GRN510, through a mechanism of telomerase activation, also suppressed the accumulation of senescent cells in the lung. (antiaging-systems.com)
  • Their function is regulated by the activation of a number of activating and inhibitory receptors that bind to specific ligands expressed on the surface of target cells. (frontiersin.org)
  • Following activation, NK cells mediate killing of target cells through two major pathways that require direct contact between NK cells and their target cells ( 6 ). (frontiersin.org)
  • 1 Significant advances have been made by combining novel agents with autologous peripheral blood stem cell transplantation which allows for long-term disease-free survival in the majority of transplant-eligible patients. (haematologica.org)
  • P21-activated kinases (PAKs) are serine/threonine kinases involved in the regulation of cell survival, proliferation, inhibition of apoptosis, and the regulation of cell morphology. (sciencegate.app)
  • Cell survival, cycling, proliferation and apoptosis were assessed by counting Trypan blue-negative cells or flow cytometry using propidium iodide (PI) and anti-annexin-V antiboidies. (unifi.it)
  • MiRNA-486 regulates angiogenic activity and survival of mesenchymal stem cells under hypoxia through modulating Akt signal[J]. Biochem Biophys Res Commun, 2016, 470:670-677. (magtechjournal.com)
  • Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber. (justia.com)
  • In particular, the invention relates to microfluidic devices and their uses and methods for assaying cells. (justia.com)
  • Existing methods for measuring transcript levels in single cells include RT-qPCR (1), single molecule counting using digital PCR (2) or hybridization probes (3, 4), and next generation sequencing (5). (justia.com)
  • Particular importance here is the biological applications of Pt NCs such as the bioimaging of various cells as a preferred fluorophore in contrast to traditional fluorescent markers (e.g., organic dye, semiconductor quantum dots, and fluorescent proteins), the usage of Pt NCs-based antitumour drugs as a new class chemotherapeutics for malignant tumour therapy, and the utilization of antibacteria as an alternative of Ag-based antibacterial agent. (hindawi.com)
  • Using a chemo-genetic approach, we identify BET bromodomain containing proteins BRD2 and BRD4 as central regulators of NK cell functions, including direct cytokine secretion, NK cell contact-dependent inflammatory cytokine secretion from monocytes as well as NK cell cytolytic functions. (frontiersin.org)
  • and Popov ex Poljakov on K562 and HL-60 cell lines. (ac.ir)
  • In conclusion, the data shown here demonstrated that CHMFL-074 was impressive against both unchanged CML cell lines and BCR-ABL positive individual major hematopoietic cells. (molecularcircuit.com)
  • The aim of this study was to evaluate the effect of methanol (MeOH), n -hexane and dichloromethane (CH 2 Cl 2 ) fractions of the extract on melanin synthesis and oxidative stress in B16F10 melanoma cell line. (ac.ir)
  • With their ability to detect and directly destroy virally infected or malignant cells, natural killer (NK) cells form an important part of the first line defense of the immune system. (frontiersin.org)
  • They can be activated rapidly via germ-line encoded receptors that recognize the presence of stress ligands or absence of self-antigens on target cells ( 1 - 5 ). (frontiersin.org)
  • BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)
  • Mutations in the Neuraminidase-Like Protein of Bat Influenza H18N11 Virus Enhance Virus Replication in Mammalian Cells, Mice, and Ferrets. (cdc.gov)
  • Flow cytometry was used to profile cell cycle alteration upon berbamine treatment. (zju.edu.cn)
  • The cell cycle arrest and apoptosis were tested by flow cytometry analysis. (biomedcentral.com)
  • In contrast, knockdown of BRD4 but not of BRD2 impairs NK cell cytolytic responses, suggesting BRD4 as critical regulator of NK cell mediated tumor cell elimination. (frontiersin.org)
  • Hypoxia prevents the cell number increase which occurred in normoxia and determined early and massive apoptosis, as well as cell cycle arrest of surviving cells. (unifi.it)
  • We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
  • JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)
  • Background Several research show that multidrug transporters such as for example P-glycoprotein (PGP) get excited about cell level of resistance to chemotherapy and refractory epilepsy. (cancerrealitycheck.com)
  • The melanogenesis inhibitory and antioxidant effects of N. satureioides on B16F10 cells may suggest this plant as a new pharmaceutical agent in reducing skin pigment and skin aging in cosmetic industry. (ac.ir)
  • Previous studies have shown that activin A inhibited the colony-forming activity of mouse Friend erythroleukemia cells, however, the mechanism remains unknown. (tmu.edu.tw)
  • Forkhead box protein M1 (FOXM1) is a key transcription factor (TF) that regulates a common set of genes related to the cell cycle in various cell types. (mdpi.com)
  • The data showed that at concentrations below 60 µg/mL, fractions did not show significant toxicity on melanoma cells. (ac.ir)
  • Natural killer (NK) cells are innate lymphocytes that play a pivotal role in the immune surveillance and elimination of transformed or virally infected cells. (frontiersin.org)
  • However, the applicability of such traditional chemotherapeutic approaches is primarily limited due to acquired Multi-Drug Resistance (MDR) in cells, which is acquired through the production of anti-apoptotic cascades and the overexpression of cell membrane-based drug efflux pumps, such as P-glycoprotein, for self-defense. (ijpsonline.com)
  • Tumor cell resistance to anthracyclines and epipodophyllotoxins can be due to reduced drug accumulation and/or alterations in the activity of topoisomerase II (TOPO II). (ucy.ac.cy)
  • These data obviously show that p38 reaches least partly mixed up in legislation of medication level of resistance in K562/Dox cells. (cancerrealitycheck.com)