• H3 controls histamine turnover by feedback inhibition of histamine synthesis and release. (wikipedia.org)
  • We have previously shown that physiological concentrations of zinc (congruent to 7 X 10(-6) M) inhibit the release of histamine from human basophil leukocytes (Marone et al. (nih.gov)
  • Preincubation (5 min, 37 degrees C) of human basophils and lung mast cells with zinc chloride (10(-6)-3 X 10(-5) M) caused dose-related inhibition of histamine and peptide leukotriene C4 (LTC4) release induced by anti-IgE. (nih.gov)
  • Zinc chloride was a competitive antagonist of the action of Ca2+ in histamine secretion induced by anti-IgE with a dissociation constant (Kd) of about 10(-5) M in both the basophil and mast cell systems. (nih.gov)
  • Thus physiological concentrations of zinc inhibit the release of histamine from human basophils and lung mast cells, presumably by blocking Ca2+ uptake induced by anti-IgE activation. (nih.gov)
  • The team knew that activation of basophil degranulation by aIgE leads to powerful mediators being released, including large amounts of histamine, which sets up a negative feedback cycle that curbs its own release. (purewatergazette.net)
  • So the experiment the pan-European team planned involved comparing inhibition of basophil aIgE-induced degranulation with "ghost" dilutions of histamine against control solutions of pure water. (purewatergazette.net)
  • In other words, they did not know whether the solutions they were adding to the basophil-aIgE reaction contained ghost amounts of histamine or just pure water. (purewatergazette.net)
  • Three of the four labs involved in the trial reported a statistically significant inhibition of the basophil degranulation reaction by the ghost histamine solutions compared with the controls. (purewatergazette.net)
  • Causes the release of histamine from basophils and activates eosinophils. (cusabio.com)
  • Evidently, proton channels are active in basophils during IgE-mediated responses and prevent excessive acidification, which may account for their role in histamine release. (johnshopkins.edu)
  • Methods: To study the biologic activity of 1-acyl-2-acetyl-GPC, we compared its effects and those of PAF on histamine and leukotriene C 4 (LTC 4 ) release from human mixed leukocytes that contained basophils. (arizona.edu)
  • Similarly, 1-acyl-2-acetyl-GPC (0.1 to 10 μmol/L) caused a concentration-dependent inhibition of PAF-activated histamine secretion (inhibitory concentration of 50% ≅ 0.2 μmol/L). Conclusions: Our data suggest that 1-acyl-2-acetyl-GPC may represent, under certain circumstances, a modulator of human basophil mediator release via mechanisms shared with PAF. (arizona.edu)
  • Likewise, basophils from a patient allergic to mugwort pollen and celery were stimulated only by native Api g 5, whereas the deglycosylated allergen did not trigger release of histamine. (lookformedical.com)
  • Urticaria results from the release of histamine, bradykinin, kallikrein, and other vasoactive substances from mast cells and basophils in the superficial dermis, resulting in intradermal edema caused by capillary and venous vasodilation and occasionally caused by leukocyte infiltration. (msdmanuals.com)
  • CD244 is expressed on NK cells, a subset of T cells (including most CD8 + T cells and γ/δ T cells), monocytes, basophils, and eosinophils. (biolegend.com)
  • Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
  • In humans, interleukin-5 is a very selective cytokine as a result of the restricted expression of the interleukin-5 receptor on eosinophils and basophils. (biomedcentral.com)
  • Eosinophils are a prominent feature in the pulmonary inflammation that is associated with allergic airway diseases, suggesting that inhibition of interleukin-5 is a viable treatment. (biomedcentral.com)
  • The β-chemokines form a gradient that serves as a chemoattractant and potential proliferation signal for immune and other cells such as monocytes, macrophages, basophils, eosinophils, T lymphocytes and fibroblasts. (justia.com)
  • All antihistamines caused a dose-dependent inhibition of TNF-α release from HMC-1 cells, with maximal effects at 10−12 M for azelastine, 10−9 M for loratadine and cetirizine, and 10−8 M for ranitidine. (citationimpact.com)
  • Most importantly, these effects of ATRA (10(-8) to 10(-6) mol/L) on CD34(+) cells were associated with a dose-dependent inhibition of IL-5Ralpha but no change in GM-CSF receptor expression, as detected with flow cytometry. (dadamo.com)
  • Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). (cusabio.com)
  • Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with Multiscreen TM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). (multispaninc.com)
  • However, stimulation of human basophils in perforated-patch configuration with PMA, N-formyl-methionyl-leucyl-phenylalanine, or anti-IgE greatly enhanced proton currents, the latter suggesting involvement of proton channels during activation of basophils by allergens through their highly expressed IgE receptor (FcεRI). (johnshopkins.edu)
  • IgE inhibition immunoblots showed that native Api g 5 other than the deglycosylated protein completely inhibited IgE binding to high molecular weight allergens in protein extracts from birch pollen, mugwort pollen, and celery. (lookformedical.com)
  • It does this by strengthening the cellular membranes of mast cells and basophils, which normally release histamines in response to allergens. (saveourbones.com)
  • What Benveniste claimed so controversially was that he continued to observe basophil degranulation even when the aIgE had been diluted out of existence, but only as long as each dilution step, as with the preparation of homoeopathic remedies, was accompanied by strong agitation. (purewatergazette.net)
  • However, either SARS-CoV-2 or SARS-CoV-2-infected epithelial cells did not alter the expression of surface markers associated with the activation of basophils, such as CD69, CD13 and/or degranulation marker CD107a. (bvsalud.org)
  • Inhibition of IgE-binding, basophil activation, and mast cell degranulation were assessed via blocking ELISA, flow cytometry, and the passive cutaneous anaphylaxis mouse model. (bvsalud.org)
  • A cocktail of all 3 antibodies, REGN5713/14/15, blocks IgE binding to Bet v 1 and inhibits Bet v 1- and birch pollen extract-induced basophil activation ex vivo and mast cell degranulation in vivo. (bvsalud.org)
  • Although allergen-induced systemic basophil degranulation was inhibited in an FcγRIIb-dependent manner, inhibition of local mast cell degranulation in tissues occurred independently of FcγRIIb. (ox.ac.uk)
  • Moreover, Qβ-Fel d1 did not induce degranulation of basophils derived from human volunteers with cat allergies. (ox.ac.uk)
  • In these experiments we compared the effect of zinc chloride on the release of chemical mediators from human basophils and mast cells isolated from human lung. (nih.gov)
  • The primary contribution of B cells to allergy is the production of IgE, the Ab isotype that triggers immediate hypersensitivity reactions through the release of mediators from mast cells and basophils. (bvsalud.org)
  • stimulates intracellular adenyl cyclase, which results in increased cyclic adenosine monophosphate levels, causing relaxation of bronchial smooth muscle and inhibition of release of mast cell mediators. (medscape.com)
  • In addition to potent and selective BTK enzyme and cellular activity, rilzabrutinib inhibited activation and inflammatory activities of B cells and innate cells such as macrophages, basophils, mast cells, and neutrophils, without cell death (in human and rodent assay systems). (aai.org)
  • FceRI cross-linking of more than 95% pure basophil preparations activates the protein-tyrosine kinases Lyn and Syk, previously linked to FceRI-coupled rodent mast cell activation, as well as Zap-70, previously implicated in T-cell receptor signaling, and causes the tyrosine phosphorylation of multiple proteins. (uncg.edu)
  • Antagonist activity of AM211 at the DP2 receptor was confirmed by inhibition of PGD 2 -stimulated guanosine 5′- O -[γ-thio]triphosphate binding to membranes expressing human DP2. (aspetjournals.org)
  • Retinoic acid modulates IL-5 receptor expression and selectively inhibits eosinophil-basophil differentiation of hemopoietic progenitor cells. (dadamo.com)
  • BACKGROUND: IL-5 plays a central role in eosinophil and basophil differentiation, exerting its effects through the IL-5 receptor (IL-5Ralpha). (dadamo.com)
  • CONCLUSIONS: These findings indicate that retinoids can differentially regulate expression of IL-5Ralpha, but not GM-CSF receptor, and that these effects have functional consequences in vitro on eosinophil and basophil differentiation. (dadamo.com)
  • Earlier HIV infec- receptor gene polymorphism in HIV infection, tion diagnosis, inhibition of ongoing HIV replica- only genes of the HLA system were thought to tion with antiretroviral therapy (in industrialized protect against HIV infection. (cdc.gov)
  • Her work using human models of disease is uncovering the impact of airway inflammation on lung function using targeted inhibition to block key cellular pathways required for developing allergen-induced airway responses. (cictrials.ca)
  • Expression of activation markers in circulating basophils and the relationship to allergen-induced bronchoconstriction in subjects with mild allergic asthma. (cictrials.ca)
  • Background: In human blood basophils, cross-linking the high-affinity IgEreceptor FceRI with multivalent antigen activates a signaling pathway leading to Ca2+ mobilization, actin polymerization, shape changes, secretion, and cytokine production. (uncg.edu)
  • A basophil activation assay and a whole-blood assay of eosinophil shape change were used to demonstrate the ability of AM211 to potently antagonize PGD 2 -stimulated functional responses in relevant human cells and in the context of a physiologically relevant environment. (aspetjournals.org)
  • Currently, little is known concerning regulation of IL-5Ralpha expression in the context of commitment of hemopoietic progenitor cells to the eosinophil and basophil lineages. (dadamo.com)
  • OBJECTIVE: Because all-trans retinoic acid (ATRA) is known to modulate some aspects of hemopoietic differentiation, we examined the effects of ATRA on eosinophil-basophil differentiation and IL-5Ralpha expression. (dadamo.com)
  • RESULTS: In semisolid methylcellulose cultures of normal human bone marrow, ATRA (10(-6) mol/L) selectively suppressed eosinophil-basophil colony-forming units but had no effect on granulocyte-macrophage colony-forming units. (dadamo.com)
  • Similarly, ATRA (10(-6) mol/L) inhibited eosinophil-basophil differentiation of cord blood CD34(+) cells in liquid culture, whereas neutrophil differentiation proceeded without impediment. (dadamo.com)
  • The basophil is a granulocyte that is developmentally closely related to the eosinophil but nevertheless does not express NADPH oxidase. (johnshopkins.edu)
  • The presence of Lyn, Syk, and Zap-70 in basophils was confirmed by Western blotting in lysates of highly purified basophils and independently by confocal fluorescence microscopy in cells labeled simultaneously with kinase-specific antibodies and with the basophil-specific antibody 2D7. (uncg.edu)
  • The process begins when an antigen induces the formation of IgE antibodies, which bind firmly with their Fc portion to the basophils and mast cells. (periobasics.com)
  • H1-type antihistamines have recently been reported to inhibit cytokine secretion from human and murine mast cells and basophils. (citationimpact.com)
  • At the mRNA level, inhibition was only seen with KU812 cells and IL-8 in the presence of azelastine at 10−10 M. These data show thus distinct inhibitory patterns for different antihistamines during cytokine production from human mast cells and basophils which may contribute to the anti-inflammatory effects of these drugs during treatment of allergic diseases. (citationimpact.com)
  • title{Protein Tyrosine Phosphatase Inhibition Mimics Fc$\varepsilon$RI-induced Anergy and Reveals the Immunomodulatory Role of Hydrogen Peroxide as a Signaling Molecule in Human Basophils\thanksref{grant}} %Really struggling with a title that captures this paper -- probably a bad sign! (openwetware.org)
  • Methods and Results: The role of tyrosine kinases in human FceRI signaling was explored by using human basophils isolated by Percoll gradient centrifugation followed by negative and/or positive selection with antibody-coated magnetic beads. (uncg.edu)
  • Conclusions: Our data demonstrate the association of anti-IgE-stimulated, protein-tyrosine phosphorylation by a cascade of tyrosine kinases, including Zap-70 as well as Lyn and Syk, with the initiation of FceRI-mediated signaling in human basophils. (uncg.edu)
  • Back in 1985, Benveniste began experimenting with human white blood cells involved in allergic reactions, called basophils. (purewatergazette.net)
  • Expression of functional cysteinyl leukotriene receptors by human basophils. (cictrials.ca)
  • We also validate that human basophils are not permissive to SARS-CoV-2 replication. (bvsalud.org)
  • However, no studies have been undertaken to investigate whether a polyphenol-rich pomegranate fruit extract (POMx) inhibits the inflammatory activity of activated human mast cells and basophils. (biomedcentral.com)
  • In addition, POMx suppressed the NF-κB activation induced by PMACI by inhibiting IκB-degradation in human basophil cells. (biomedcentral.com)
  • However, tyrosine phosphorylation continues to increase at high anti-IgE concentrations that elicit very little secretion (the characteristic high-dose inhibition of secretion). (uncg.edu)
  • Learn more about the kinase inhibition application in the webinar below! (buhlmannlabs.com)
  • Inhibition ELISA assays demonstrated that each of the hydrolysates had decreased capacity to bind peanut-specific IgE compared with nonhydrolyzed controls. (oit101.org)
  • In this study, we report that SARS-CoV-2 induces cytokine responses and in particular IL-13, in both resting and IL-3 primed basophils. (bvsalud.org)
  • Our data suggest that basophil cytokine responses to SARS-CoV-2 might help in reducing the inflammation and also to promote antibody responses to the virus. (bvsalud.org)
  • Selective BTK inhibition has the potential to target multiple immune-mediated disease pathways. (aai.org)
  • Our unique marker combination - CCR3 and CD63 - combines the simple gating properties for basophil detection by CCR3 with the solid application of the proven activation marker CD63 in one. (buhlmannlabs.com)
  • Mast cells and basophils are multifunctional effector cells and contain plentiful secretary granules in their cytoplasm. (biomedcentral.com)
  • Objective: To investigate whether FAHF-2 inhibits mast cell/basophil numbers and IgE-mediated activation. (mssm.edu)
  • The major sesame allergen was identified by nanoLC-MS/MS. Cross-reactivity was measured using an immuno-inhibition assay with the Phadia ImmunoCAP system. (wur.nl)
  • Western blotting, inhibition ELISA and basophil activation tests were used to examine IgE reactivity. (oit101.org)
  • PF670462 or dexamethasone also ameliorated allergic symptoms in a mouse model of allergic rhinitis and downregulated allergen-specific basophil reactivity in patients with allergic rhinitis. (elsevierpure.com)
  • Objective We determined whether pharmacologically resetting the molecular clock in mast cells or basophils to times when FcϵRI signaling was reduced (ie, when core circadian protein period 2 [PER2] is upregulated) resulted in suppression of IgE-mediated allergic reactions. (elsevierpure.com)
  • Conclusion Pharmacologically resetting the molecular clock in mast cells or basophils to times when FcϵRI signaling is reduced can inhibit IgE-mediated allergic reactions. (elsevierpure.com)
  • According to the researchers, RA's anti-allergy effects are "due to two independent mechanisms: inhibition of the inflammatory response and scavenging of ROS [reactive oxygen species]. (saveourbones.com)
  • Conclusion: Food allergy herbal formula 2 reduction of basophils and mast cell numbers as well as suppression of IgE-mediated mast cell activation may contribute to FAHF-2's persistent protection against peanut anaphylaxis. (mssm.edu)
  • These novel pharmacological actions of POMx provide new suggestion that POMx or POMx-derived compounds may be of therapeutic use for the treatment of inflammatory diseases by suppressing mast cells/basophils activation. (biomedcentral.com)
  • Mast cells and basophils are known to play a central role in inflammatory and immune events [ 1 ]. (biomedcentral.com)
  • The pro-inflammatory cytokines produced by the mast cells and basophils play an important role in the development of acute- and late-phase inflammatory reactions. (biomedcentral.com)
  • These data suggest that inhibition of mast cell/basophil function could provide benefit in RA and other inflammatory diseases. (biomedcentral.com)
  • These studies demonstrate that IPI-145 exerts profound effects on adaptive and innate immunity by inhibiting B and T cell proliferation, blocking neutrophil migration, and inhibiting basophil activation. (buhlmannlabs.ch)
  • According to the American National Cancer Institute, crude plant extracts are considered cytotoxic in an in vitro assay when concentrations 20 μg/mL and below produce 50% inhibition of tumor cells, after an exposure time of 48 hours. (ru.ac.za)
  • Basophils play a key role in the orientation of immune responses. (bvsalud.org)
  • Comparable amounts of Lyn and Syk were found in basophils and B cells, whereas T cells appear to have greater amounts of Zap-70 than basophils. (uncg.edu)
  • The immunological background of the sesame seed and tree nut IgE sensitization was characterized with Western blotting and a basophil activation test (BAT). (wur.nl)
  • Peripheral blood basophil numbers and peritoneal mast cell numbers and FcεRI expression were determined. (mssm.edu)
  • Though increased expression of immune checkpoint molecule PD-L1 has been reported on the basophils from COVID-19 patients, we observed that SARS-CoV-2 does not induce PD-L1 on the basophils. (bvsalud.org)
  • which results from mast cell and basophil activation in the deeper dermis and subcutaneous tissues and manifests as edema of the face and lips, extremities, or genitals. (msdmanuals.com)
  • Quercetin has shown a biphasic behavior in basophils at nanomolar doses and hence its action on cells involved in allergic inflammation. (saveourbones.com)
  • The BAT and ImmunoCAP inhibition test added value to the clinical and immunological characterization of sesame seed-sensitized patients, distinguishing relevant and non-relevant sensitizations. (wur.nl)
  • Thymic stromal lymphopoietin activation of basophils in patients with allergic asthma is IL-3 dependent. (cictrials.ca)