• It is a protein with a key role in regulating muscle differentiation or myogenesis, specifically the development of skeletal muscle. (wikipedia.org)
  • It functions during that time to commit myogenic precursor cells to become skeletal muscle. (wikipedia.org)
  • While the product of this gene is capable of directing cells towards the skeletal muscle lineage, it is not absolutely required for this process. (wikipedia.org)
  • As one of the key cell markers of satellite cells (the stem cell pool for skeletal muscles), it plays an important role in the regeneration of adult muscle. (wikipedia.org)
  • Skeletal muscle contains a number of different progenitor cells, including satellite and mesenchymal progenitor cells. (hindawi.com)
  • Inhibition of protein synthesis and activation of degradation metabolism can both lead to the development of skeletal muscle atrophy, a pathological condition characterized by a decrease in muscle mass and fiber size. (bvsalud.org)
  • Therefore, it is necessary to develop and use cell models to understand the etiology and influencing factors of skeletal muscle atrophy. (bvsalud.org)
  • Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
  • Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)
  • Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)
  • Yet, it remains unclear from murine transplantation experiments which cell population most effectively engrafts into diseased skeletal muscle. (biomedcentral.com)
  • Thrown for a loop: fibro-adipogenic progenitors in skeletal muscle fibrosis. (ucdavis.edu)
  • Studies previously conducted show that HS negatively affects the skeletal muscle growth and development by changing its effects on myogenic regulatory factors, insulin growth factor-1, and heat-shock proteins. (frontiersin.org)
  • For postnatal development and regeneration of skeletal muscle mass satellite television cells Mirtazapine a self-renewing pool of muscle mass stem cells give rise to child myogenic precursor cells that contribute to the formation of fresh muscle mass fibers. (mdm-inhibitors.com)
  • Fracture Healing in Diabetes Fracture repair entails recruitment of mesenchymal stem cells after which differentiation to chondrocytes that type cartilage (see Chapter 12). (dnahelix.com)
  • Adult mesenchymal stem cells (MSCs) represent a subject of intense experimental and biomedical interest. (biomedcentral.com)
  • A number of different local transcription factors called myogenic regulatory factors (MRFs), including MyoD, myf-5, myogenin, and myf-6, stimulate proliferation and differentiation of satellite cells [ 9 ]. (hindawi.com)
  • In addition, insulin, such as growth factor (IGF) 1Ea and/or IGF-1Ec (MGF), also stimulates proliferation and differentiation of satellite cells. (hindawi.com)
  • Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K. "Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. (sciepub.com)
  • Therefore, promotion of myoblast proliferation and differentiation and induction of myotube hypertrophy should be beneficial for muscle regeneration and control of muscle mass. (kosfaj.org)
  • WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
  • Enriched monolayer precursor cell cultures from micro-dissected adult mouse dentate gyrus yield functional granule cell-like neurons. (mdc-berlin.de)
  • Myf5 is expressed in the dermomyotome of the early somites, pushing the myogenic precursors to undergo determination and differentiate into myoblasts. (wikipedia.org)
  • Regeneration of muscle after injury normally occurs rapidly with satellite stem cells that reside in the space between the basal lamina and the sarcolemma, playing a major role [ 11 ]. (hindawi.com)
  • Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
  • The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
  • Mesenchymal stromal cell spheroids in sulfated alginate enhance muscle regeneration. (ucdavis.edu)
  • Yamashita D, Machigashira M, Miyamoto M, Takeuchi H, Noguchi K, Izumi Y, Ban S. "Effect of surface roughness on initial responses of osteoblast-like cells on two types of zirconia. (sciepub.com)
  • Medium pH modulates matrix, mineral, and energy metabolism in cultured chick bones and osteoblast-like cells. (sciepub.com)
  • ATGL in beta cells regulates insulin secretion via the production of signalling MAGs. (omicsdi.org)
  • This signaling pathway is a highly conserved cell-to-cell communication system that regulates cell fate in development and disease. (5dok.org)
  • Eph/ephrin signaling is a cell-to-cell communication pathway, which regulates cell migration and proliferation. (5dok.org)
  • Despite only being present for a few days during embryonic development, Myf5 is still expressed in certain adult cells. (wikipedia.org)
  • Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)
  • IL-2/IL2R also promotes the differentiation of T cells into effector T cells. (ilreceptor.com)
  • Ex vivo cultures of myofiber explants demonstrate that ablation of IRE1α reduces the proliferative capacity of myofiber-associated satellite cells. (elifesciences.org)
  • These cells are located beneath the basal lamina of myofiber in a dormant state. (elifesciences.org)
  • Myf5 belongs to a family of proteins known as myogenic regulatory factors (MRFs). (wikipedia.org)
  • The compromised tissue repair program is attributable to the gradual depletion of stem cells and to altered regulatory signals. (biomed.news)
  • Semaphorins are a wide family of extracellular regulatory signals with a multifaceted role in cell-cell communication. (biomed.news)
  • In the presence of antler extracts, the length and thickness of myotubes and myogenin differentiation 1 (MyoD1) and myogenic factor 5 (Myf5) gene expression were increased compared to those in the control group (CON). (kosfaj.org)
  • Gene expression of AMP-activated protein kinase (AMPK), MyoD1, and myogenin, along with the muscle atrophy factors muscle RING finger-1 (MuRF-1) and forkhead box O3a (FoxO3a) upon addition of deer antler extracts to muscle-atrophied C2C12 cells was determined by qRT-PCR after treatment with AICAR. (kosfaj.org)
  • In addition, gene expression of MyoD1 and myogenin in the muscle atrophy cell model was significantly increased compared that into the CON. (kosfaj.org)
  • Therefore, our findings indicate that antler extract can increase the expression of MyoD1, Myf5 and myogenin, inhibit muscle atrophy, and promote muscle differentiation. (kosfaj.org)
  • From a research, medical, and business standpoint, mesenchymal stem cell (MSC)-based therapies are fascinating. (biomedcentral.com)
  • Serra M, Brito C and Alves PM. "Bioengineering strategies for stem cell expansion and differentiation. (sciepub.com)
  • Therefore, these results establish the efficacy of diprotin A in stimulating muscle cell engraftment, and highlight the pre-clinical utility of a xenotransplantation model in assessing the relative efficacy of muscle stem cell populations. (biomedcentral.com)
  • We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
  • On the cover: Human induced pluripotent stem cell-derived neuroepithelial-like stem cells stained for actin (green) and DNA (blue). (5dok.org)
  • We used conjugated polymers to develop a novel neural stem cell culture substrate with anchored growth factors to promote cell self-renewal. (5dok.org)
  • The inhibition and augment of WNT pathway in vitro further verified our results. (ijbs.com)
  • Results of panobinostat on target gene methylation and expression in vitro We following investigated whether the inhibition of DNMT activity and expression is additionally reflected about the methyla tion pattern of recognized hypermethylated tumor suppres sor genes. (ilreceptor.com)
  • Since only small numbers of cells are obtainable from the patient, it requires expansion of cells in vitro . (sciepub.com)
  • Nevertheless, these primary cells grow slowly in vitro and lose their general characteristics during prolonged culturing period. (sciepub.com)
  • Using biologically relevant elastic substrates to study cell function in vitro has proven beneficial, as the in vivo microenvironment usually is much softer than rigid plastic dishes. (5dok.org)
  • MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation. (mdc-berlin.de)
  • The limb myogenic precursors (derived from the hypaxial myotome) do not begin expressing Myf5 or any MRFs, in fact, until after migration to the limb buds. (wikipedia.org)
  • Still, the existence of these separate "MyoD-dependent" and "Myf5-dependent" subpopulations has been disputed, with some claiming that these MRFs are indeed coexpressed in muscle progenitor cells. (wikipedia.org)
  • However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
  • In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)
  • Some evidence shows that Myf5 and MyoD are responsible for the development of separate muscle lineages, and are not expressed concurrently in the same cell. (wikipedia.org)
  • A beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion in mice. (omicsdi.org)
  • To directly assess the role of beta cell lipolysis in insulin secretion and whole-body energy homeostasis, inducible beta cell-specific adipose triglyceride lipase (ATGL)-deficient (B-Atgl-KO) mice were studied under normal diet (ND) and high-fat diet (HFD) conditions. (omicsdi.org)
  • The results support the concept that fuel excess can drive obesity and diabetes via hyperinsulinaemia, and that an islet beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion. (omicsdi.org)
  • Understanding the mechanisms controling BAT differentiation may identify potential therapeutic targets to enhance BAT-controlled metabolism and to increase EE. (biomedcentral.com)
  • Laboratory work has centered on the mechanisms of how cells acquire specific fates during growth and development, providing insights into normal and abnormal mechanisms of growth control. (stanford.edu)
  • While much is known about biochemical signaling, many of the mechanisms that drive cell outcome in response to biophysical influences remain to be uncovered. (5dok.org)
  • However, the mechanisms of cell proliferation induced by LPLI are poorly understood. (biomedcentral.com)
  • Adult stem cells play key roles in homeostasis and tissue repair. (biomed.news)
  • In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
  • The response of target cells is further regulated by their microenvironment, involving the extracellular matrix, which may be modified by MSC-produced matrix metalloproteinases (MMPs) and tissue inhibitor of MMPs. (biomedcentral.com)
  • Cells sense and respond to the mechanics of their substrate - be it the extracellular matrix, neighboring cells or artificial matrix in cell culture. (5dok.org)
  • The cell membrane is really a viscous fluid-like matter which includes several lipids, cholesterol, and inserted proteins. (globaltechbiz.com)
  • LPLI-induced cell cycle progression can be regulated by the activation or elevated expressions of cell cycle-specific proteins. (biomedcentral.com)
  • Recently, a large number of signaling proteins reported play an important key role in the process of LPLI-induced cell proliferation, probably due to the fact that the molecular events they are involved in are the basic response of the cells to extracellular stimuli. (biomedcentral.com)
  • For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but can transition to an activated, proliferative state upon injury. (biomed.news)
  • Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)
  • While acknowledging progress in the other areas mentioned, this work will focus on the current debates concerning sourcing, MSC alterations of angiogenesis, cell differentiation/stimulation, and strategies to improve MSC differentiation. (biomedcentral.com)
  • Myogenic factor 5 is a protein that in humans is encoded by the MYF5 gene. (wikipedia.org)
  • In clinical practice, these involve the life-long immunosuppression of the organ transplantation patients, the creation of the supporting environment for engineered tissues in the mended organ, the unsolved issues of cell survival and differentiation of the cell-based therapy, and the selection and development of vectors for gene therapy. (biomedcentral.com)
  • Upon an electrochemical switch, growth factor presentation was reversed, which initiated cellular differentiation along the neuronal lineages. (5dok.org)
  • Differentiation begins (regulated by other genes) after this initial proliferation. (wikipedia.org)
  • In addition, the expression levels of genes related to muscle differentiation and atrophy were confirmed through qRT-PCR. (kosfaj.org)
  • Most notably, while Myf5 is activated by Sonic hedgehog in the epaxial lineage, it is instead directly activated by the transcription factor Pax3 in hypaxial cells. (wikipedia.org)
  • We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)
  • Cytoskeleton, U-93631 which is a network of interconnected filaments of different types, connects the cell membrane with underlying sub-cellular components. (globaltechbiz.com)
  • The nucleus is the largest organelle among sub-cellular components, demonstrating solid-elastic behavior [4], and it is typically stiffer than the cell itself [5]. (globaltechbiz.com)
  • Liver kinase B1 (LKB1) plays pivotal roles in cellular processes such as apoptosis, metabolism, and cell cycle regulation. (omicsdi.org)
  • Low power laser irradiation (LPLI) promotes proliferation of multiple cells, which (especially red and near infrared light) is mainly through the activation of mitochondrial respiratory chain and the initiation of cellular signaling. (biomedcentral.com)
  • Although it is expressed in both the epaxial (to become muscles of the back) and hypaxial (body wall and limb muscles) portions of the myotome, it is regulated differently in these tissue lines, providing part of their alternative differentiation. (wikipedia.org)
  • Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
  • MSCs can be defined by their ability to adhere to tissue culture plastic, their expression of several cell surface molecular epitopes-cluster of differentiation CD73, CD90, and CD105, and others-as well as their lack of several surface markers, including CD45 [ 6 ]. (biomedcentral.com)
  • Tissue-engineering and cell-based therapies, two prominent branches of Regenerative Medicine, are considered as the most suitable techniques to repair the tissue damage. (sciepub.com)
  • Commensurate with the known truth that HuR improved the great quantity of adhesion substances VLA-4 on Th17 cells, knockout of HuR impaired splenic Th17 cell migration towards the central anxious program and abolished the condition. (globaltechbiz.com)
  • Our results show that we can tune cell stiffness and migration by regulating Notch activity and matrix stiffness. (5dok.org)
  • Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)
  • Furthermore, Myf5 is a master regulator of muscle development, possessing the ability to induce a muscle phenotype upon its forced expression in fibroblastic cells. (wikipedia.org)
  • In fact, if Myf5 is not downregulated, differentiation does not occur. (wikipedia.org)
  • Tumor cells remodel the microenvironment suitable for their proliferation, wherein they communicate with fibroblast cells to modulate their expression and induce tumor progressive cytokines. (bvsalud.org)
  • We propose an opportunity to target the cancer cell/microenvironment interface instead of the Notch pathway itself in the development of cancer therapies. (5dok.org)
  • Secondly, they can improve our understanding of the adjustments that occur during disease progression which, in turn, might uncover reasons for corresponding alterations occurring in cell mechanics [10, 11]. (globaltechbiz.com)
  • Discoveries in the our lab over the past 25 years have shown that immune cells, especially myeloid cells, play a major role in modulating muscle injury and repair that occur in chronic, muscle wasting diseases and following acute injuries. (ucla.edu)
  • These changes take place on the molecular level impacting properties of specific the different parts of cell inner structure, but resulting in alterations in mechanical properties of the complete cell ultimately. (globaltechbiz.com)
  • Engineered multi-functional, pro-angiogenic collagen-based scaffolds loaded with endothelial cells promote large deep burn wound healing. (ucdavis.edu)
  • Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
  • Recent mechanistic insights into the trophic activities of these cells point to ultimate regulation by nitric oxide, nuclear factor-kB, and indoleamine, among other signaling pathways. (biomedcentral.com)
  • These MSC influences can range from relatively rare activities that require cell contact, such as mitochondrial transfer and cell fusion, to relatively common paracrine MSC actions through extracellular microvesicles or secreted factors. (biomedcentral.com)
  • Recombinant production of growth factors for application in cell culture. (au.dk)
  • The intent of vaccination is to induce a combined antibody and T-cell anti-HER-2 immune response, which is intended to target HER-2-expressing tumor cells, and may induce tumor regression or slow progression of disease. (stanford.edu)
  • Yamada K. "Biological effects of low power laser irradiation on clonal osteoblastic cells (MC3T3-E1). (sciepub.com)
  • Cell proliferation is a very important physiological effect for low power laser irradiation (LPLI) used in clinical practice. (biomedcentral.com)
  • In line with all the reduction of methylation, an greater expression of APC was observed in each cell lines, reaching the highest degree at 48 h for Hep3B and at 72 h for HepG2, respectively. (ilreceptor.com)
  • We used the xenotransplant model to show that CXCR4 expression on canine donor muscle cells is also important for cell engraftment, but FACS sorting for CXCR4-positive cells decreased their engraftment efficiency. (biomedcentral.com)
  • The light must be absorbed by the endogenous chromophores of cells or tissues for actions. (biomedcentral.com)
  • Attachment of human marrow stromal cells to titanium surfaces. (sciepub.com)
  • For example, our findings have shown that macrophages and eosinophils are key effector cells in the pathogenesis of Duchenne muscular dystrophy. (ucla.edu)
  • Ongoing investigations in the lab are revealing the identity of specific molecules released by myeloid cells that promote muscular dystrophy. (ucla.edu)
  • Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. (sciepub.com)
  • These cells are regulated by a tight control of transcriptional programs. (biomed.news)
  • Matrix stiffness and architecture drive fibro-adipogenic progenitors' activation into myofibroblasts. (ucdavis.edu)
  • In this study, we used a canine-to-murine xenotransplantation model to quantitatively compare canine muscle cell engraftment, and test the most effective cell population and modulating factor in a canine model of DMD using allogeneic transplantation experiments. (biomedcentral.com)
  • In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)
  • Yamaguchi DT, Huang JT, Ma D. "Regulation of gap junction intracellular communication by pH in MC3T3-E1 osteoblastic cells. (sciepub.com)
  • Here we have investigated biophysical regulation of cell function. (5dok.org)
  • At 8 weeks of age, these mice were injected with tamoxifen to induce deletion of beta cell-specific Atgl (also known as Pnpla2), and the mice were fed an ND or HFD. (omicsdi.org)
  • Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)
  • Because of this alternative use of MSCs, Caplan and Sorell [ 14 ] suggested a renaming of MSCs to medicinal signaling cells to suggest a new era of MSC clinical relevance due to their immunomodulatory properties. (biomedcentral.com)