Loading...
  • cells and biofilms
  • However, the transcriptional response to the hydrocarbons was similar between suspended cells and biofilms, with two genes up-regulated in both growth states in response to phenol (NE1545-NE1546) while no transcriptional response was observed during toluene exposure. (oregonstate.edu)
  • completely inhibited
  • The results indicated that the effect of farnesol was dependent on the concentration of this compound and the initial adherence time, and preincubation with 300 μM farnesol completely inhibited biofilm formation. (asm.org)
  • concentration
  • Extensive structural changes in established biofilms were seen in the sample with less than or equal to 30%(w/v) Medihoney using scanning electron microscopy and loss of viability was found in test samples with less than or equal to 20%(w/v) Medihoney concentration using fluorescent staining, together with loss of biofilm structure. (cardiffmet.ac.uk)
  • a threshold concentration triggers the formation of a biofilm ( 17 , 27 ). (asm.org)
  • The dry weight, protein concentration, number of cfu, and polysaccharide composition per biofilm were determined. (oup.com)
  • Increasing the dissolved oxygen (DO) concentration in the biofilms resulted in higher NH₃ oxidation rates and greater phenol inhibition, leading to the conclusion that the tolerance of biofilms is likely related to O₂ limitation causing slow NH₃ oxidation rates and slower growth rates. (oregonstate.edu)
  • DO and pH were measured in the biofilms with microsensors and the concentration profiles were simulated with 2-D reactive transport that combined advective and diffusive transport. (oregonstate.edu)
  • Maggot ES at 100 mg/mL concentration significantly reduced biofilm formation thus disrupting established biofilm of E. cloacae . (hindawi.com)
  • vitro
  • To address these challenges, we fabricated poly(lactic- co -glycolic acid) (PLGA) and methoxy-polyethylene glycol PLGA (mPEG-PLGA) nanoparticles (NPs) that encapsulate BAR peptide, and assessed the potency of BAR-encapsulated NPs to inhibit and disrupt in vitro two-species biofilms. (biomedcentral.com)
  • Moreover
  • Moreover, QQ-2 chemically immobilized to the glass surface of the flow cell effectively inhibited biofilm formation of K. oxytoca as well as clinical K. pneumoniae isolates derived from patients with urinary tract infections. (frontiersin.org)
  • Furthermore
  • Furthermore, mixed species of biofilms have complementary metabolic strategies for obtaining nutrients and degrading host immune molecules [ 14 ]. (hindawi.com)
  • protein
  • Northern blot analysis of RNA extracted from cells in biofilms indicated that the levels of expression of HWP1 , encoding a hypha-specific wall protein, were decreased in farnesol-treated biofilms compared to the levels in controls. (asm.org)
  • Although CDI systems have so far only been demonstrated to be involved in interbacterial competition, constitutive production of the system's immunity protein in the entire bacterial population did not alter biofilm formation, indicating a CDI-independent role for BcpA in this process. (nih.gov)
  • To our knowledge, this report of amyloid-dependent biofilm inhibition by a human protein with no significant bactericidal or bacteriostatic activity is unique. (pnas.org)
  • persistence
  • Biofilm growth and its persistence within wounds have recently been suggested as being a contributing factor towards impaired healing [ 3 , 8 ]. (hindawi.com)
  • species
  • Ström-Bestor and Wiklund 2011 ), however, species of microbes included in biofilms can interact in various ways with other species within the same biofilm (Moons et al. (springeropen.com)
  • While Macrophen and Double Macrophen were the most active substances among polyamino-phenolic ligands, dendrimers were overall twofold more effective than all other compounds with a reduction up to 85 and 73% of legionella and multi-species biofilms, respectively. (frontiersin.org)
  • In addition, the kinetics of BAR-encapsulated NPs were compared after different durations of exposure in a two-species biofilm model, against previously evaluated BAR-modified NPs and free BAR. (biomedcentral.com)
  • mutans
  • We investigated the influence of apigenin and tt -farnesol, alone and in combination, on the accumulation, polysaccharide composition and viability of S. mutans UA159 biofilms. (oup.com)
  • The recoverable viable counts of S. mutans were slightly lower (0.5 to 1 log 10 decrease in cfu/biofilm) after apigenin and tt -farnesol treatments compared with the vehicle control. (oup.com)
  • Apigenin and tt -farnesol affected the accumulation and polysaccharide content of S. mutans biofilms without major impact on the bacterial viability. (oup.com)
  • extracellular
  • Biofilms treated with the test agents also resulted in lower amounts of extracellular alkali-soluble glucans, intracellular iodophilic polysaccharides and, to a lesser extent, fructans. (oup.com)
  • genes
  • Analysis of a strain constitutively expressing the genes indicates that native expression is critical for biofilm architecture. (nih.gov)
  • Using RT-qPCR, genes up-regulated in the biofilm microarray were detected in continuous cultures of suspended cells, and were increasingly up-regulated with decreasing dilution rates. (oregonstate.edu)
  • substrates
  • Candidiasis associated with intravenous lines and bioprosthetic devices is problematic, since these devices can act as substrates for biofilm growth. (frontiersin.org)
  • biomass
  • B) For each of the strains in (A), COMSTAT analysis of Z stacks collected after 24 (white bars) or 72 h (gray bars) of biofilm development was used to determine biofilm total biomass (left panel), average thickness (middle panel), and maximum thickness (right panel). (nih.gov)
  • gene expression
  • This dissertation explores the physiology and gene expression of the ammonia-oxidizing bacterium Nitrosomonas europaea in surface-associated bacterial communities, or biofilms. (oregonstate.edu)
  • The observations suggest a correlation between the biofilm-related gene expression and slow growth rates of the cells. (oregonstate.edu)
  • kinetics
  • The half-saturation coefficient for suspended cells fit the biofilm profiles, indicating that the kinetics of NH₃ oxidation between the biofilm and suspended cells were the same. (oregonstate.edu)
  • bacterium
  • Legionnaires' disease is a potentially fatal pneumonia caused by Legionella pneumophila , an aquatic bacterium often found within the biofilm niche. (frontiersin.org)