Inhibition arrest of cells cell cycle. Medical search. Frequent questions

It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
It is a protein kinase which plays an important role in mitotic cell cycle progression. (wikipedia.org)
A novel UCS cell line (PF338) was established from the malignant pleural effusion of a 59-year-old patient at time of disease progression. (por-journal.com)
Importantly, UCS is the prototype tumor for epithelial-mesenchymal transition (EMT), a reversible biological process that associates with tumor progression and metastasis and in which epithelial cells transform into more invasive mesenchymal cells by losing their epithelial properties [ 10 - 12 ]. (por-journal.com)
This effect is mediated by downregulation of the key cell cycle progression factors cyclin B1, cyclin D1, CDK1, CDK4, and survivin, the latter also constituting an essential inhibitor of apoptosis, underlying additionally the onset of apoptosis. (uandes.cl)
Some studies showed that blocking cell cycle progression ameliorated fibrosis. (nature.com)
The encoded protein plays a role in many cellular processes including cell cycle progression, DNA repair and apoptosis by regulating the activity of protein phosphatase 1. (nih.gov)
PLoS Genetics Research Article featuring Mol Onc trainees Aziz Aiderus, Justin Y. Newberg, Ana Contreras-Sandoval, and Amanda Meshey and Mol Onc faculty Karen M. Mann and Michael B. Mann: Transposon mutagenesis identifies cooperating genetic drivers during keratinocyte transformation and cutaneous squamous cell carcinoma progression. (moffitt.org)
Knockdown of Annexin A2 significantly suppressed cell proliferation and invasion of NSCLCs cells, and the biological effect of Annexin A2 on proliferation and invasion was mediated by the inhibition of p53, which in turn, promoted cell cycle progression and EMT. (jcancer.org)
In cancer progression, FOXO3a inhibition stimulates cell transformation and angiogenesis ( 9 ). (spandidos-publications.com)
T he presence and activity of various proteins are required in the cell cycle progression. (ukessays.com)
We studied the effect of doxorubicin (Dox) on cell cycle progression and its correlation with DNA damage and cytotoxicity in p53-mutant P388 cells. (aspetjournals.org)
In untreated control P388 cells, p34cdc2 kinase localizes in the nucleus and cytoplasms, particularly in the centrosomes, and p34cdc2 kinase activity is dependent on cell cycle progression, with the enzyme activity increasing steadily from G1/S to G2/M and markedly declining thereafter. (aspetjournals.org)
This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. (cancerindex.org)
Together, these findings elucidate a prosurvival role for cyclin D3-CDK6 in metabolism, in addition to its role in cell-cycle progression, and suggest that high levels of cyclin D3 and CDK6 may predict response to CDK4/6 inhibitors. (aacrjournals.org)

Interestingly, P-Tex cells expressed CDK4 genes as high as cancer cells, which could be simultaneously inhibited by CDK4 inhibitors and might be a potential reason for the ineffectiveness of CDK4 inhibitors in treating HPV-positive HNSCC. (elifesciences.org)
This study provides fundamental insight into the functional impact of CDK4 inhibition on cells in the tumor microenvironment, which is of high importance and interest to the field. (elifesciences.org)
Inhibitors of the cyclin-dependent kinases CDK4 and CDK6 induce cell-cycle arrest in RB1-proficient tumors and have had promising results in several tumor types. (aacrjournals.org)
Thus, the CDK4/6 inhibitor palbociclib reduced NADPH and GSH levels in T-ALL cells, thereby increasing ROS levels to induce apoptosis, which could be rescued by the antioxidant N -acetyl-cysteine. (aacrjournals.org)
Moreover, in breast cancer cells, which express CDK4 instead of CDK6, palbociclib induced cell-cycle arrest instead of apoptosis, further indicating that expression of cyclin D3 and CDK6 in T-ALL cells promotes apoptosis in response to palbociclib. (aacrjournals.org)
Additionally, 16 of 18 nonleukemic cancer cell lines exhibiting high expression of cyclin D3 and CDK6 underwent apoptosis in response to palbociclib, and, in melanoma patient-derived xenografts, high cyclin D3 and CDK6 expression was associated with tumor regression after CDK4/6 inhibition. (aacrjournals.org)
As normal cells entered density-dependent arrest, cyclin D1 decreased while cyclin D2 was induced and replaced D1 in Cdk4 complexes. (ku.dk)
Yet, at an elevated density the Ras-transformed cells ceased to proliferate and entered a quiescent-like state with low Cdk4 and Cdk2 activity. (ku.dk)

Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
In turn, the plant produces GA, which seems to combat the improper growth signals and re-establishes cell-cycle control. (nature.com)
Due to the role of oncogenic transcriptional activators NFĸB and STAT3 in survivin protein expression, and APE1/Ref-1 redox activity regulating their transcriptional activity, we assessed selective inhibition of APE1/Ref-1's redox function as a novel method to halt prostate cancer cell growth and survival. (oncotarget.com)
Whereas immunohistochemistry of the metastatic tumor showed a predominantly sarcomatous vimentin positive tumor that has lost E-cadherin expression, PF338 cells demonstrated biphasic growth and carried mutations in KRAS , PIK3CA , PTEN and ARID1A . (por-journal.com)
As proof-of-concept, we show that in vivo knockdown of ASncmtRNAs results in drastic inhibition of tumor growth in a xenograft model of MDA-MB-231 subcutaneous tumors, further supporting this approach for the development of new therapeutic strategies against breast cancer. (uandes.cl)
The growth rate of the cells was reduced when grown in ( significantly? (racetab.org)
As there is a substantial growth-inhibitory aftereffect of leucine deprivation, we explored whether leucine deprivation got any inhibitory influence on cell routine development. (racetab.org)
In the present study, the TCEE treatment induced cell cycle arrest and suppressed cell growth on both Hep3B and HepJ5 cells. (hindawi.com)
The encoded protein is likely associated with apoptosis, cell cycle arrest, growth inhibition or cell differentiation. (nih.gov)
By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
The inhibition of cell growth was also demonstrated by cell cycle arrest at G2/M phase. (greenmedinfo.com)
Conversely, FOXO3a overexpression suppresses cancer cell growth, induces apoptosis, and reduces tumor size by regulating downstream effectors ( 10 ). (spandidos-publications.com)
It is an alkylating agent, and its mechanism of action of active metabolites may involve cross-linking of DNA, which may interfere with growth of normal and neoplastic cells. (medscape.com)
these 2 events, in turn, can inhibit growth of neoplastic cells. (medscape.com)
Reversible proteasome inhibition disrupts pathways supporting cell growth, thus decreasing cancer cell survival. (medscape.com)
Moreover, synergistic growth inhibition was identified in a panel of 5 mouse and 7 human PDAC cell lines using both Bliss Independence and Loewe models. (nih.gov)
HL-60 cells incubated with various amounts (1, 3, 5, 7.5, and 10 micro l/mL) of the extracts for 1-3 days showed dose-dependent, time-dependent growth suppression and decrease in cell viability. (nih.gov)
From these four compounds, the esterified enantiomer (R)-5a was active in cells, which was evidenced by the increase of p53 levels, the induced expression of p53-target genes (CDKN1A and MDM2), the selective induction of cell cycle arrest, and selective growth inhibition of p53 wt U-2 OS and SJSA-1 compared to p53 del SAOS-2 cells. (rcsb.org)
1) For example, the down-regulation of miR15s and miR - 16 - 1 in chronic lymphocytic leukaemia, prostate cancer and pituitary tumours, leading to the inhibition of tumour growth and induce cell cycle arrest at the G 1 - phase by target cell cycle regulators ( cyclin D1, cyclin E1, cyclin D3 and CDK6). (ukessays.com)
If the estrogen receptor (ER) is present in breast cancer cells, estrogen enters into tumor cells and binds with ER, promoting mRNA and DNA synthesis of tumor cells, stimulating tumor cell growth. (lookchem.com)
A structure-activity relationship was uncovered with increasing cell growth inhibition as side chain length increases at the ortho and para positions of the parent DHP molecule. (bvsalud.org)
Growth inhibition was consistent across n = 21 rhabdomyosarcoma cell line models. (bvsalud.org)

Our data suggest that HDAC inhibition is effective in a novel UCS cell line by interfering with both viability and differentiation. (por-journal.com)
We further detected the gene expression of proinflammatory cytokines in Mφ and genes related to cell differentiation in MSC during the challenge of cPE.Our results demonstrated that the oxidative phosphorylation of Mφ exposed to cPE was significantly decreased when compared with the control group. (stanford.edu)
Let-7 Let-7 has an essential role in cell cycle and the differentiation of cell cycle terminals. (ukessays.com)
Neuronal differentiation and cell-cycle programs mediate response to BET-bromodomain inhibition in MYC-driven medulloblastoma. (cancerindex.org)
The inhibitors demonstrated promising effects in vitro with reduced viability, increased differentiation and cell death of neuroblastoma cells. (lu.se)

Viability assays and cell cycle analyses were used to test in vitro sensitivity to different standard and novel treatments. (por-journal.com)
Three of the four cell lines were sensitive by in vitro cytotoxicity assays to the c-Src inhibitor dasatinib, which led to cell cycle arrest and increased apoptosis. (mesothelioma-line.com)
Moreover, treatment with TCEE is found to enhance the cytotoxic effects of amphotericin B in human colon cancer cell both in vitro and in vivo [ 17 ]. (hindawi.com)
However, whether casticin inhibits in vitro carcinogenesis and cancer stem cell (CSC) characteristics, and whether casticin activates FoxO3a in small cell lung cancer (SCLC) cells remain unclear. (spandidos-publications.com)
Emerging evidence suggests that cancer stem cells (CSCs), a subpopulation of tumor cells, have the properties of self-renewal, heterogeneous progeny, drug-resistance, and carcinogenesis in vitro and in vivo ( 4 , 5 ). (spandidos-publications.com)
However, whether casticin inhibits in vitro carcinogenesis and CSC characteristics in the SCLC H446 cell line, and activates FoxO3a remains unclear. (spandidos-publications.com)
elicits antiproliferative and proapoptotic activities in vitro in solid and hematologic tumor cells. (medscape.com)
We investigated the ability of the ATR inhibitor, AZD6738, to prevent the gemcitabine-induced intra-S-phase checkpoint activation and evaluated the antitumor potential of this combination in vitro and in vivo In PDAC cell lines, AZD6738 inhibited gemcitabine-induced Chk1 activation, prevented cell-cycle arrest, and restrained RRM2 accumulation, leading to the strong induction of replication stress markers only with the combination. (nih.gov)
Moreover, this is the first paper to clearly establish that a CD56 bright CD3 - CD16 - perforin- NK reg population associates with a lack of cGvHD and has several unique characteristics, including the suppression of helper T-cell function in vitro. (haematologica.org)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Dasatinib also inhibited migration and invasion independent of the cytotoxic effects, and led to the rapid and durable inhibition of c-Src and its downstream pathways. (mesothelioma-line.com)
P-Tex cells could aggregate in the antigen-presenting cell niches and activate certain signaling pathways. (elifesciences.org)
In this chapter, we will focus on IL-21's biological effects and signaling pathways as well as discuss the potential implications and applications of IL-21 in leukemia cells. (intechopen.com)
The mechanisms of radioresistance are still poorly understood, despite it has been suggested that miRNAs play an important role in cell signaling pathways. (frontiersin.org)
Indeed, it has been shown that miRNAs play an important role in gene expression, mainly when associated with the monitoring of several cell and metabolic pathways, being also an essential component of the gene silencing machinery in most eukaryotic organisms ( 4 , 8 ). (frontiersin.org)
Present study was designed to determine the impact of cadmium on cell proliferation, cell cycle and apoptosis, and to investigate the possible involvement of p53 and MAPKs signaling pathways in J774A.1 murine macrophages. (cdc.gov)

Open up in another window Shape 3 Leucine deprivation induces apoptosis of human being breast tumor BAY 293 cell(A and B) MDA-MB-231 or MCF-7 cells had been incubated in charge (+leu) or leucine-deficient (?leu) moderate for 48h, accompanied by dedication of cleaved caspases inside a and annexin-V assay in B. (C and D) Nude mice. (racetab.org)
This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)
Ganoderma lucidum inhibits proliferation and induces apoptosis in human prostate cancer cells PC-3. (greenmedinfo.com)
Casticin, a natural polymethoxyflavone isolated from A. annua, V. trifolia, and V. agnus‑castus induces apoptosis in cancer cells by activating FoxO3a. (spandidos-publications.com)
However, in T-cell acute lymphoblastic leukemia (T-ALL), which predominately expresses CDK6 and the activating cyclin, cyclin D3, inhibition of CDK6 or cyclin D3 induces apoptosis. (aacrjournals.org)
It is immunotoxic in vivo and induces apoptosis in many cultured cells. (cdc.gov)

Our study demonstrates that survivin and APE1/Ref-1 are significantly higher in human prostate cancer specimens compared to noncancerous controls and that APE1/Ref-1 redox-specific inhibition with small molecule inhibitor, APX3330 and a second-generation inhibitor, APX2009, decreases prostate cancer cell proliferation and induces cell cycle arrest. (oncotarget.com)
Reishi inhibits proliferation and induces programmed cell death in human prostate cancer cells. (greenmedinfo.com)

The survival prognosis of human papillomavirus (HPV)-positive and HPV-negative head and neck squamous cell carcinoma (HNSCC) is largely different, and little is known about the anti-tumor mechanism of tumor-infiltrated exhausted CD8 + T cells (Tex) in HNSCC. (elifesciences.org)
NSCLCs including adenocarcinoma, squamous cell carcinoma and large cell carcinoma, constitute 80% of all lung cancers [ 2 ]. (jcancer.org)
HPV infections have received particular attention in recent years, as high-risk strains have been linked to some cases of oral squamous cell carcinoma. (medscape.com)

One of the treatments applied in cancer is radiotherapy (RT), a therapeutic modality that uses ionizing radiation to induce damage in unwanted cells. (frontiersin.org)
In vivo , palbociclib induced apoptosis of human T-ALL cells, but did not induce apoptosis in T-ALL cells expressing phosphomimetic PFK1 and PKM2 mutants. (aacrjournals.org)
Conclusion: The present findings indicate that the prostanoid FP or the EP2 receptor agonist may solely and differently induce the planar and subepithelial proliferation of HconF cells and these were also modulated by TGF-ß2. (bvsalud.org)
Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)

The treatment also induces an increase in the microRNA hsa-miR-4485-3p, whose sequence maps to ASncmtRNA-2 and transfection of MDA-MB-231 cells with a mimic of this miRNA induces cyclin B1 and D1 downregulation. (uandes.cl)
Apoptosis-related protein-1 acts as a tumor suppressor in cholangiocarcinoma cells by inducing cell cycle arrest via downregulation of cyclin-dependent kinase subunits. (nih.gov)
Our data demonstrate that Ganoderma lucidum inhibits cell proliferation in a dose- and time-dependent manner by the down-regulation of expression of cyclin B and Cdc2 and by the up-regulation of p21 expression. (greenmedinfo.com)
The translocation t(11;14)(q13;q32) is considered the precipitating oncogenic event that induces cell cycle deregulation in mantle cell lymphoma due to overexpression of cyclin D1 . (wikidoc.org)
Cell cycle-dependent cytotoxicity, G2/M phase arrest, and disruption of p34cdc2/cyclin B1 activity induced by doxorubicin in synchronized P388 cells. (aspetjournals.org)
Our results suggest that anthracycline-induced cytotoxicity is cell cycle dependent and is mediated, at least in part, by disturbance of the regulation of p34cdc2/cyclin B1 complex, thus leading to G2/M phase arrest. (aspetjournals.org)
Cyclin D3-CDK6 inhibits the glycolytic enzymes PFK1 and PKM2 to prevent T-ALL cell apoptosis. (aacrjournals.org)
The mechanisms underlying the prosurvival function of cyclin D3-CDK6 have not been elucidated, prompting Wang and colleagues to search for substrates that may promote cancer cell survival. (aacrjournals.org)
The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival. (aacrjournals.org)
Surprisingly, this delayed arrest was molecularly distinct from contact inhibition of normal cells, as it occurred in the absence of p27 Kip1 induction and cyclin D1 levels remained high. (ku.dk)

Moreover, we found that knockdown of Annexin A2 significantly suppressed cell proliferation and invasion of NSCLCs cells. (jcancer.org)

Strikingly, histone deacetylase (HDAC) inhibition markedly reduced cell viability by inducing a dose-dependent G0/1 arrest and led to mesenchymal-epithelial transition as evidenced by morphological change and increased E-cadherin and β-catenin expression. (por-journal.com)
Rigosertib reduced cell viability of neuroblastoma cells and caused cell cycle arrest in the G2/M phase. (lu.se)

One potential therapeutic target, the nonâ€"receptor tyrosine kinase c-Src, causes changes in proliferation, motility, invasion, survival, and angiogenesis in cancer cells and may be a valid therapeutic target in MPM. (mesothelioma-line.com)
Ataxia telangiectasia and Rad3-related protein (ATR), the apical kinase of the intra-S-phase DNA damage response, plays a central role in safeguarding cells from replication stress and can therefore limit the efficacy of antimetabolite drug therapies. (nih.gov)
In a cell-free system, Dox did not inhibit p34cdc2 kinase activity, indicating that is has no direct effect on the enzyme function. (aspetjournals.org)
In whole cells, Dox treatment prevented p34cdc2 kinase dephosphorylation without altering its synthesis, and this effect was due to neither down-regulation of cdc25C nor inhibition of protein-tyrosine phosphatase activity. (aspetjournals.org)
A good correlation was found between extent of DNA double-strand breaks and p34cdc2 kinase activity inhibition. (aspetjournals.org)

The ras oncogene transforms immortalized, contact-inhibited non-malignant murine fibroblasts into cells that are focus forming, exhibit increased saturation density, and are malignant in suitable hosts. (ku.dk)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

BAY 293 We looked into the cytotoxicity of leucine deprivation on regular cells further, including major hepatocytes, brownish adipose cells (BAT) and MCF-10A cells. (racetab.org)
Furthermore, the combined drug effects of TCEE with conventional chemotherapy agents, cisplatin and doxorubicin, were also analyzed to clarify whether TCEE enhances or antagonizes the cytotoxicity of the selected chemotherapy agents in hepatocellular carcinoma cells. (hindawi.com)
MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) tests demonstrated that the GA-mPEG 2000 micelle formulation had obvious cytotoxicity to tumor cells and human umbilical vein endothelial cells. (dovepress.com)
Pretreatment with PD98059 increased Cd-induced cytotoxicity suggesting that ERK activation may be a survival response in the present cell system. (cdc.gov)
Cycloheximide, an inhibitor of protein synthesis, did not alter Cd-induced cytotoxicity indicating that J774A.1 cell death by Cd is independent of de novo protein synthesis including p53. (cdc.gov)

Our findings suggest that DIM may ameliorate hyperglycemia and diabetic nephropathy through the inhibition of PKC-α and TGF-β1 signaling. (researchgate.net)

In this study, we explored the role of Plk1 in the classical kidney fibrosis model, UUO, and cultured fibroblast and renal tubular epithelial cells by using specific Plk1 inhibitors, genetic silencing, and heterozygous global knockout mice. (nature.com)
Expression of cell cycle inhibitors, P21 and P27, and activation of apoptosis executer enzyme, caspase-3, were also induced by TCEE. (hindawi.com)
Increasing evidence indicates that senescent cells could be a promising new target for therapeutic intervention known as senotherapy, which includes depleting senescent cells, modulating SASP and restoration of senescence inhibitors. (frontiersin.org)
Our results suggest that Ras pathway inhibitors in combination with KSP inhibition represent a promising treatment strategy against neuroblastoma. (lu.se)

Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)

Combination of Pin1 inhibitor with ralaniten promoted cell cycle arrest and had improved antitumor activity against CRPC xenografts in vivo compared to individual monotherapies. (bcgsc.ca)
The cell-free media from MWCNT-polarized macrophages induced the migration of neutrophilic cells (differentiated from HL-60), which was blocked by Acebilustat, a specific leukotriene A4 hydrolase inhibitor, or LY239111, an LTB4 receptor antagonist, but not NS-398, a cyclooxygenase 2 inhibitor, revealing LTB4 as a major mediator of neutrophil chemotaxis from MWCNT-polarized macrophages. (cdc.gov)

We hypothesized that impaired immunoregulation and bone regeneration from inflammatory states are associated with glycolytic reprogramming and mitochondrial dysfunction in macrophages (Mφ) and mesenchymal stromal cells (MSCs).We used the Seahorse XF96 analyzer and real-time qPCR to study the bioenergetics of Mφ and MSCs exposed to cPE. (stanford.edu)
Scattered non-granumloma forming epithelioid macrophages , hyalinized vessels and a few reactive T cells or mast cells are seen along with the lymphoma cells in MCL . (wikidoc.org)
Various immune-suppressive cell populations appear to associate with suppression of cGvHD, including T reg cells, B reg cells, M2 macrophages, dendritic cells, and CD56 bright natural killer (NK) cells. (haematologica.org)

KSP inhibition caused tumor regression in vivo and resulted in increased survival of multiple PDX models. (lu.se)

In contrast, the antitumor effects and related biological mechanism of TCEE as well as the combination drug effects with conventional chemotherapy agents remain unclear particularly in human hepatocellular carcinoma cells. (hindawi.com)
However, the mechanism(s) responsible for the effects of Ganoderma lucidum on cancer cells remain to be elucidated. (greenmedinfo.com)
These findings reveal a novel mechanism for regulating AMPK/FoxO3a signaling in response to casticin, suggesting a new strategy for SCLC therapy by targeting cancer stem‑like cells. (spandidos-publications.com)
We also found that viable NK reg cells may be isolated by sorting on CD56 + and CD16 - NK cells, and this population can suppress allogeneic CD4 + T cells, but not T reg cells or CD8+ T cells through a non-cytolytic, cell-cell contact dependent mechanism. (haematologica.org)
With further investigation we may decipher the mechanism of NK reg suppression and operationalize expansion of NK reg cells associated with cGvHD suppression. (haematologica.org)
This demonstrates that although oncogenic Ras efficiently disabled the normal response to contact inhibition, a separate back-up mechanism enforced cell cycle arrest at higher cell density. (ku.dk)

This TCEE also demonstrates antitumor properties such as the induction of cell cycle arrest and activation of apoptosis on human colon, lung, melanoma, osteosarcoma, and pancreatic cancer cells [ 16 - 19 ]. (hindawi.com)
Its roles include regulation of proliferation, promotion of immune system and activation of apoptosis in B cells. (intechopen.com)

Open up in another window Shape 2 Leucine deprivation inhibits breasts tumor cell proliferation(A) MDA-MB-231 or CORIN MCF-7 cells had been seeded in 12-well plates and incubated in charge (+leu) or leucine-deficient (?leu) moderate. (racetab.org)
Vincristine inhibits cellular mitosis by inhibition of intracellular tubulin function, binding to microtubules, and synthesis of spindle proteins in the S phase. (medscape.com)

In addition, Plk1 also prevented partial epithelial-mesenchymal transition (pEMT) of tubular epithelial cells via autophagy pathway. (nature.com)
In HHV-1 and HHV-2 oral infections, viral replication within the oral epithelium may cause lysis of epithelial cells, with vesicle formation. (medscape.com)

Because APE1/Ref-1 is expressed and elevated in prostate cancer, we sought to characterize APE1/Ref-1 expression and activity in human prostate cancer cell lines and determine the effect of selective reduction-oxidation (redox) function inhibition on prostate cancer cells in vitro and in vivo . (oncotarget.com)

Treatment of neuroblastoma PDX cells with ARRY-520 resulted in mitotic arrest and subsequent cell death. (lu.se)

Outcomes Leucine deprivation decreases viability of tumor cells We 1st determined the result of leucine deprivation on viability of tumor cells. (racetab.org)
Open up in another window Shape 1 The result of leucine deprivation on cell viability(A and B) Cells had been incubated in charge BAY 293 (+leu) or leucine-deficient (?leu) moderate for indicated period, accompanied by MTT assay. (racetab.org)
First, Cell Counting Kit-8 assay was performed to evaluate the effects of various concentrations of ATO on the viability of HTFs. (bvsalud.org)

The aims of this study are to clarify the antitumor effects of TCEE on human hepatocellular carcinoma cells and also evaluate the combination drug effects with conventional chemotherapy agents, cisplatin and doxorubicin. (hindawi.com)
The aims of this preclinical study are to evaluate the capability of TCEE to suppress human hepatocellular carcinoma cells and clarify the related antitumor effects. (hindawi.com)

Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
In UCS, several studies identified characteristic EMT-related expression signatures including active TGF-β signaling in tissue and cell lines [ 7 , 11 , 13 , 14 ]. (por-journal.com)
To test this hypothesis, we determined the effects of c-Src inhibition in MPM cell lines and examined c-Src expression and activation in tissue samples. (mesothelioma-line.com)
The Src protein was highly expressed in tumor cells, but expression did not correlate with survival. (mesothelioma-line.com)
Furthermore, the expression of LC3 ‑ II, an autophagy marker, in these cells also increased post treatment with ASHE. (lab-sunchlorella.com)
The expression of pro-apoptotic proteins and anti-apoptotic proteins in MCF-7 and MDA-MB-231 cells was investigated by Western blotting. (researchsquare.com)
We have previously demonstrated that Ganoderma lucidum down-regulated the expression of NF-kappaB-regulated urokinase plasminogen activator (uPA) and uPA receptor (uPAR), which resulted in suppression of cell migration of highly invasive human breast and prostate cancer cells. (greenmedinfo.com)
Furthermore, Ganoderma lucidum induced apoptosis of PC-3 cells with a slight decrease in the expression of NF-kappaB-regulated Bcl-2 and Bcl-xl. (greenmedinfo.com)
Mechanistically, our studies showed that knockdown of Annexin A2 increased the expression of p53, which in turn, induced cell cycle G2 arrest and inhibited epithelial-to-mesenchymal transition (EMT). (jcancer.org)
The inhibition of Pin1 expression or its activity selectively reduced the transcriptional activities of full-length AR and AR-V7. (bcgsc.ca)
Combination therapies using ralaniten with ionizing radiation were evaluated for effects on proliferation, colony formation, cell cycle, DNA damage, and Western blot analyses in human prostate cancer cells that express both full-length AR and AR-Vs. Ralaniten and a potent next-generation analog (EPI-7170) decreased expression of DNA repair genes whereas enzalutamide had no effect. (bcgsc.ca)
Furthermore, Mφ and MSCs exposed to cPE showed upregulated gene expression levels of glycolytic regulators and Mφ exposed to cPE expressed higher levels of pro-inflammatory cytokines.This study demonstrated the dysfunctional bioenergetic activity of bone marrow-derived Mφ and MSCs exposed to cPE, which could impair the immunoregulatory properties of cells in the bone niche. (stanford.edu)
Western blotting was used for protein expression analysis of the collected cell masses (spheroids), and H-E staining was used for structural observation. (medsci.org)
Cisplatin showed concentration-dependent antitumor effects due to loss of cell adhesion and spheroid disruption in each cell line, while cetuximab exhibited antitumor effects that correlated with EGFR expression in each cell line. (medsci.org)
It regulates all cell types' functions and is an important second messenger that controls and triggers several mechanisms, including membrane stabilization, permeability, contraction, secretion, mitosis, intercellular communications, and in the activation of kinases and gene expression. (bvsalud.org)

In UCS, TP53 has been identified as the most frequently mutated gene, followed by mutations in the PI3K pathway, KRAS , cell cycle regulators including FBXW7 and chromatin remodeling and histone genes including ARID1A [ 7 - 9 ]. (por-journal.com)
VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)
The proteasome pathway is an enzyme complex existing in all cells, which degrades ubiquitinated proteins that control the cell cycle and cellular processes and maintains cellular homeostasis. (medscape.com)
Inhibition of PFK1 and PKM2 promotes pentose phosphate and serine pathway production of antioxidants. (aacrjournals.org)

This work adds to the elucidation of the molecular mechanisms behind cell cycle arrest preceding tumor cell apoptosis induced by ASncmtRNA knockdown. (uandes.cl)
The purpose of BAY 293 our current research would be to investigate the result of leucine deprivation on breast tumor cells and also to elucidate the root mechanisms. (racetab.org)
In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
The signaling mechanisms of IL-21 in different cell types have been identified. (intechopen.com)
However, we know less about the biological effects of IL-21 and its signaling mechanisms in leukemia cells and monocytes. (intechopen.com)
Thus, Ganoderma lucidum exerts its effect on cancer cells by multiple mechanisms and may have potential therapeutic use for the prevention and treatment of cancer. (greenmedinfo.com)
1 - 4 Multiple mechanisms may be involved in its potent antitumor effect, including induction of apoptosis, cell cycle arrest, telomerase inhibition, antiangiogenesis activity, and an antimetastasis effect. (dovepress.com)

Cd at 20 microM markedly increased cells in G2/M and hypodiploid sub-G1 phases of the cell cycle suggesting cycle arrest and cell death. (cdc.gov)

We found Plk1 plays a critical role in maintaining intralysosomal pH by regulating ATP6V1A phosphorylation, and inhibition of Plk1 impaired lysosomal function leading to blockade of autophagic flux. (nature.com)
Glycolytic reprogramming, the shift from oxidative phosphorylation (OXPHOS) to glycolysis causes inappropriate cell activation and function, resulting in dysfunctional cellular metabolism. (stanford.edu)

The ethanolic extract of T. camphoratus (TCEE) which contains abundant bioactive compounds including triterpenoids and polysaccharides also has antitumor effects in various human cancer cell lines. (hindawi.com)

In this study, we investigated the effects of Ganoderma lucidum on cell proliferation, cell cycle, and apoptosis in human prostate cancer cells PC-3. (greenmedinfo.com)
Ralaniten Sensitizes Enzalutamide-Resistant Prostate Cancer to Ionizing Radiation in Prostate Cancer Cells that Express Androgen Receptor Splice Variants. (bcgsc.ca)
Ralaniten and EPI-7170 sensitized prostate cancer cells that express full-length AR and AR-Vs to radiotherapy whereas enzalutamide had no added benefit. (bcgsc.ca)

Inhibition of APE1/Ref-1 redox function significantly reduced NFĸB transcriptional activity, survivin mRNA and survivin protein levels. (oncotarget.com)
The binding of miRNA- protein complexes to mRNA causes translation inhibition or destabilisation of target transcripts. (ukessays.com)

These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)

Inhibition of activity results in apoptosis. (wikipedia.org)
In conclusion, ASHE exerts cytostat ic activity on liver cancer cells via both apoptosis and autophagy, and may serve as a potential therapeutic agent for management of liver cancer and autophagy ‑ related diseases. (lab-sunchlorella.com)
Antagonizing MDM2 with small molecules restores the activity of p53 in p53 wild-type (p53 wt ) cells and thus provides positive outcomes in the treatment of p53 wt cancers. (rcsb.org)
Inhibition of ERK suppressed DNA synthesis and had an additive effect with Cd-inhibited proliferation suggesting that Cd-induced ERK activity is not responsible for the G2/M arrest and subsequent inhibition of cell proliferation. (cdc.gov)

Here, we show that ASncmtRNA knockdown induces cell death preceded by proliferative blockage in three different human breast cancer cell lines. (uandes.cl)
Establishment of six new human biliary tract carcinoma cell lines and identification of MAGEH1 as a candidate biomarker for predicting the efficacy of gemcitabine treatment. (nih.gov)
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced on VALD-3. (researchsquare.com)
We performed cell-level multi-omics sequencing on human HNSCC samples to decipher the multi-dimensional characteristics of Tex cells. (elifesciences.org)
Interleukin-21 (IL-21) was first identified in 2000 through screening of a cDNA library from CD3+ human T cells [ 1 ]. (intechopen.com)
In this study, we showed that Annexin A2 was evidently overexpressed in human NSCLCs cell lines and NSCLCs tissues. (jcancer.org)
Different volumes of 70% ethanol and water extracts of I'm-Yunity were incubated with cultures of human promyelocytic leukemic HL-60 cells, and compared to nontreated control cells. (nih.gov)

Scholars@Duke publication: Inhibition of Isoprenylcysteine Carboxylmethyltransferase Induces Cell-Cycle Arrest and Apoptosis through p21 and p21-Regulated BNIP3 Induction in Pancreatic Cancer. (duke.edu)

Our results suggest that ASncmtRNA targeting blocks tumor cell proliferation through reduction of essential cell cycle proteins, mediated by mitochondrial and nuclear miRNAs. (uandes.cl)

I'm-Yunity acts selectively in HL-60 leukemic cells, resulting in cell cycle restriction through the G(1)/S checkpoint and the induction of apoptosis. (nih.gov)
Additionally, NK reg cells do not kill leukemic cells. (haematologica.org)

SncmtRNA is expressed in all proliferating cells, while ASncmtRNAs are expressed in normal proliferating cells, but is downregulated in tumor cells. (uandes.cl)

Involved in G2/M phase cell cycle arrest induced by DNA damage. (wikipedia.org)
ASHE suppressed liver cancer cell proliferation by inducing cell cycl e arrest at the G0/G1 phase, as well as apoptosis, as indicated by t he increased number of Annexin V and 7 ‑ AAD ‑ positive cells. (lab-sunchlorella.com)
proliferation and cell cycle phase distribution, (2). (nih.gov)
Flow cytometric analysis revealed partial cell arrest in the G(1) phase at less than 5 micro L/mL and induction of apoptosis at 10 micro L/mL or more of ethanol and water extracts, with the latter exhibiting more pronounced inhibition than the former. (nih.gov)
Treatment of synchronized cells in early S phase with 1 microM Dox (2 x ID50) for 1 hr induced a marked cell arrest at G2/M phase at 6-12 hr after drug incubation. (aspetjournals.org)

Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
Pdx-Cre (KPC) mouse cancer cell line, significantly extending survival. (nih.gov)

In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)

FOXO3a is considered an evolutionarily conserved transcription factor involved in various cellular processes, including cell cycle arrest, DNA repair and tumor suppression ( 8 ). (spandidos-publications.com)

Furthermore, di-GA inhibited the generation of lymphendothelial gaps by cancer cell spheroid-secreted lipoxygenase metabolites. (nature.com)
May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence. (wikipedia.org)
Four varieties of tumor cells were examined: malignant melanoma A375, lung caner A549, ovarian carcinoma breast and A2780 cancer MCF-7 and MDA-MB-231 cells. (racetab.org)
In the pres ent study, the inhibitory effect of the root extract of ASH (ASHE) on HuH ‑ 7 and HepG2 liver cancer cells was examined. (lab-sunchlorella.com)
Fluoresce nce and transmission electron micrographs of ASHE ‑ treated liver cancer cells showed the presence of an increased number of autophagic vesicles. (lab-sunchlorella.com)
Due to its key role in the eukaryotic cell cycles, Plk1 has been one of the most validated drug targets for cancer treatment. (nature.com)
Flow cytometry analysis showed that VALD-3 triggered cell cycle arrest and induced apoptosis of breast cancer cells. (researchsquare.com)
The compelling conclusion that proliferative exhausted T cells are associated with response in HPV+ head and neck cancer is supported by the cohort of 14 patients with paired tumor and adjacent normal tissue and rigorous bioinformatic analysis of nearly 50,000 single CD3+ T cell transcriptomes. (elifesciences.org)
Annexin A2 has been involved in cancer cell adhesion, invasion and metastasis. (jcancer.org)
We here demonstrated that casticin decreased sphere‑ and colony‑formation capabilities, and downregulated uPAR and CD133 in second‑generation spheres, which were considered as lung cancer stem‑like cells (LCSLCs), from SCLC H446 cells, in a concentration‑dependent manner. (spandidos-publications.com)
Small cell lung cancer (SCLC), a poorly differentiated and highly aggressive tumor, constitutes approximately 15% of all lung cancers ( 1 ). (spandidos-publications.com)
Cancer research has been conducted using cultured cells as part of drug discovery testing, but conventional two-dimensional culture methods are unable to reflect the complex tumor microenvironment. (medsci.org)
Three-dimensional cultures were performed using several oral cancer cell lines. (medsci.org)
Spheroids made from oral cancer cell lines appeared to have tumor-like characteristics that may reflect their clinical significance. (medsci.org)
Furthermore, it is presumed that most of the characteristics inherent in cancer cells are lost. (medsci.org)
However, Tamoxifen Z isomer enters into the cell, competitively binding with ER to form receptor complexes, inhibiting that estrogen plays an role, and inhibiting proliferation of breast cancer cells. (lookchem.com)
Basal cell carcinoma (BCC) is a nonmelanocytic skin cancer (ie, an epithelial tumor) that arises from basal cells (ie, small, round cells found in the lower layer of the epidermis). (medscape.com)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

Apoptotic death is preceded by proliferation blockage, suggesting that these transcripts are involved in cell cycle regulation. (uandes.cl)
As main findings, 23 miRNAs were already identified as being involved in genetic regulation of PCa cell response to RT. (frontiersin.org)
1) The regulation of these protein levels is vital for the understanding of the cell cycle control and its dysregulation. (ukessays.com)

Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
The main goal of RT consists in delivering a precise dose of radiation in a target volume, such as tumor, promoting the tumor cells eradication with as minimal damage as possible in surrounding normal tissues ( 13 ). (frontiersin.org)
2 cGvHD is a multisystemic disorder that occurs due to foreign donor immune cells attacking the recipient's tissues. (haematologica.org)

A proliferative exhausted CD8 + T cell cluster (P-Tex) which was beneficial to survival outcomes of patients with HPV-positive HNSCC was identified. (elifesciences.org)

Chronic graft- versus -host disease (cGvHD) is a major cause of morbidity after hematopoietic stem cell transplantation (HSCT). (haematologica.org)
Hematopoietic stem cell transplantation (HSCT) is an important therapeutic option for patients with non-malignant diseases as well as high-risk and refractory hematopoietic malignancies. (haematologica.org)
Hematopoietic Stem Cell Transplantation Hematopoietic stem cell (HSC) transplantation is a rapidly evolving technique that offers a potential cure for hematologic cancers ( leukemias, lymphomas, myeloma) and other hematologic disorders. (msdmanuals.com)
Living donors are often used for kidney and hematopoietic stem cell transplants, less frequently for segmental liver transplants, and rarely for pancreas and lung transplants. (msdmanuals.com)

We aimed to isolate and define the characteristics of regulatory NK (NK reg ) cells associated with suppression of cGvHD. (haematologica.org)

2) Therefore, the proper control of protein levels is crucial for the cell cycle. (ukessays.com)

Follicular dendritic cells (FDC) are a hallmark of mantle cell lymphoma and may also be involved in its pathogenesis . (wikidoc.org)

Annexin A2 (also called ANXA2) is mainly expressed in endothelial cells. (jcancer.org)

Anatomy25

Organisms13

Diseases4

Chemicals and Drugs113

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes82

Disciplines and Occupations1

Information Science3

Health Care2

Progression15

CDK48

Growth23

Differentiation5

Vitro9

Pathways7

Induces apoptosis6

Proliferation and induces2

Squamous cell carc3

Induce4

Cyclin10

Suppressed cell proliferation1

Reduced cell viability2

Kinase5

Fibroblasts2

Cytotoxicity5

Diabetic nephropathy1

Inhibitors4

Tumour2

Inhibitor2

Macrophages3

Vivo1

Mechanism6

Activation of apoptosis2

Inhibits2

Epithelial cells2

Selective1

Mitotic1

Viability3

Hepatocellular carcinoma2

Expression15

Pathway4

Mechanisms7

Markedly1

Phosphorylation2

Antitumor1

Prostate cancer3

MRNA2

Disruption1

Activity4

Human7

Pancreatic1

Proteins1

Leukemic cells2

Downregulated in tumor cells1

Phase5

Significantly2

Leukaemia1

Cellular processes including1

Cancer19

Regulation3

Tissues4

Survival1

Hematopoietic4

Suppression1

Protein levels1

Dendritic cells1

Endothelial cells1