• Since tetrahydrofolate is needed for synthesis of purine and thymidine synthesis, it results in the inhibition of DNA and RNA synthesis. (toku-e.com)
  • Synchronized hepatoma tissue culture (HTC) cells, accumulated at the G1/S boundary with aminopterin, were released into S phase with either thymidine or 5-bromodeoxyuridine (BUdR). (rupress.org)
  • The fused cells had been cultured in trophoderm-containing hypoxanthine-aminopterin-thymidine (Head wear) moderate and 15% FBS. (glex2017.org)
  • Around the same time, and building on the observation that the vitamin folic acid could stimulate acute lymphoblastic leukemia (ALL) cells, Farber used folate analogs such as aminopterin and then amethopterin (methotrexate) to treat ALL, in what is often heralded as the first 'rational' drug development approach [ 4 ]. (biomedcentral.com)
  • It irreversibly binds to dihydrofolate reductase, inhibiting the formation of reduced folates, and thymidylate synthetase, resulting in inhibition of purine and thymidylic acid synthesis. (demegaformulations.info)
  • The immunosuppressive effect of MZB has been reported to be due to the inhibition of DNA synthesis in the S phase of the cell cycle. (hindawi.com)
  • There are several proposed mechanisms of action for MTX, including the inhibition of a number of enzymes, such as methylenetetrahydrofolate reductase, which can lead to an interference in DNA synthesis. (neoteryx.com)
  • Methotrexate began to replace the more powerful and toxic antifolate aminopterin starting in the 1950s, and the two should not be confused with each other. (demegaformulations.info)
  • The drug moieties may affect cytotoxic and cytostatic mechanisms including tubulin binding, DNA binding, or topoisomerase inhibition. (justia.com)
  • 2. Inhibition of the growth of Lewis lung carcinoma by indomethacin in conventional, nude, and beige mice. (nih.gov)
  • This had happened despite treatment with an experimental drug, developed at Boston Children's Hospital by Farber and his team, working on the assumption that inhibition of folate metabolism should slow the growth of tumor cells. (medscape.com)