Infected with a bacterial biofilm. Medical search. Frequent questions

Biofilms have great importance for public health because of their role in certain infectious diseases and importance in a variety of device-related infections. (cdc.gov)
Specially tailored viruses could eradicate chronic bacterial infections. (technologyreview.com)
Biofilms, which often form on hard surfaces such as catheters and water pipes, can cause gingivitis and chronic ear infections. (technologyreview.com)
Large doses of antibiotics can usually eradicate these infections, but there is some worry that drug--resistant biofilm infections are becoming more common. (technologyreview.com)
Mr. Salka's leadership will enable AmpliPhi to execute on its mission to develop innovative therapeutic solutions aimed at the growing problem of combating antibiotic resistant bacterial infections," said Mr. Curnock Cook. (businesswire.com)
With a strong proprietary technology platform, first-in-class cGMP manufacturing capabilities and excellent lead pipeline candidates for addressing a major global health problem, AmpliPhi is strongly positioned to bring new therapies for treating deadly bacterial infections to market. (businesswire.com)
Bacterial biofilms play an important role in UTIs, responsible for persistent infections leading to recurrences and relapses. (mdpi.com)
Like a protective tent over a colony of harmful bacteria, biofilms make the treatment of skin infections especially difficult. (eurekalert.org)
Microorganisms protected in a biofilm pose a significant health risk due to their antibiotic resistance and recalcitrance to treatment, and biofilm-protected bacteria account for some 80 percent of total bacterial infections in humans and are 50 to 1,000 times more resistant to antibiotics than simpler bacterial infections. (eurekalert.org)
This work could have especially useful applications for military medical treatments, he noted, where soldiers in the field can be exposed to bacterial infections that are particularly difficult to treat. (eurekalert.org)
Bacterial infections in the skin are among the most common diagnoses in hospital patients, accounting for some 10% of all hospital visits. (eurekalert.org)
The biofilm-associated infections caused by even drug-sensitive bacteria are very difficult to treat, and patients tend to have recurrent infections [2]. (databasefootball.com)
During bacterial infections involving biofilms, most of the existing antibiotics can kill the susceptible, planktonic bacterial population present in various body sites. (databasefootball.com)
The SCVs too have been implicated in recurrence of various bacterial infections. (databasefootball.com)
Estimations made by the Centers for Disease Control and Prevention assert that 65 percent of hospital-acquired infections involve biofilms. (healthjockey.com)
As the ESF Biofilm workshop's convenor Tom J. Battin, from the University of Vienna, pointed out, biofilms are involved in most chronic infections, including killers such as cystic fibrosis, and endocarditis in the heart. (sciencedaily.com)
To further investigate the pathogenesis of NTHi in respiratory infections, we developed a novel in vitro coculture model of NTHi biofilm formation on polarized human airway epithelial cells grown at the air-liquid interface. (nih.gov)
These potential complications have been illustrated in a recent article in the CDC's Morbidity and Mortality Weekly Report (MMWR), Serious Bacterial Infections Acquired During Treatment of Patients Given a Diagnosis of Chronic Lyme Disease . (scienceblogs.com)
Understanding these oral floras can help in assessing various dental diseases like gingivitis and periodontitis which account for the most frequent bacterial infections in the human body. (news-medical.net)
More importantly, these microorganisms can even result in serious systemic infections like bacterial endocarditis, preterm labor, pneumonia, and cardiovascular diseases. (news-medical.net)
Fungal infections in the oral cavity can be a result of the growth of fungal biofilms. (news-medical.net)
Understanding the biological characteristics of biofilms, the cause of most known bacterial infections, is the first step to fight against this silent pandemic and to find effective treatments. (ibecbarcelona.eu)
Bacterial infections are a public health problem affecting millions of people worldwid e, and about 80% of them are associated with biofilms , communities formed by different microorganism s' species which co-colonize human tissues and medical devices. (ibecbarcelona.eu)
Due to several biological characteristics , b iofilm s a re much more recalcitrant than single bacterial infections and need longer periods and higher antibiotic doses to be treated. (ibecbarcelona.eu)
Only with the continuous development and improvement of efficient antibiofilm strategies we can tackle the recurrence and chronicity caused by P. aeruginosa biofilm infections. (ibecbarcelona.eu)
Eduard Torrents, leader of the "Bacterial infections: antimicrobial therapies" group at IBEC and professor at UB. (ibecbarcelona.eu)
It is also a relevant pathogen in chronic wounds infections and skin injuries , and in keratitis, a common corneal infection related with bacterial biofilm formation over contact lenses. (ibecbarcelona.eu)
Bacterial biofilms are formed by different species that colonize and grow together on human tissues, causing infections in the urinary tract, lung and kidney among others, and on surfaces of medical devices, as joint prostheses , heart valves and pacemakers, dental and breast implants, sutures and catheters. (ibecbarcelona.eu)
There are many approaches being used to control infections by suppressing its formation but CRISPR-CAS (gene editing technique) and photo dynamic therapy (PDT) are proposed to be used as therapeutic approaches to subside bacterial biofim infections, especially caused by deadly drug resistant bad bugs. (biomedcentral.com)
Therefore, biofilm matrix gives the additional resistance power to bacteria which makes them to not only tolerate harsh conditions but also resistant to antibiotics which lead to the emergence of bad bugs infections like multi drug resistant, extensively drug resistant and totally drug resistant bacteria. (biomedcentral.com)
Since eradication of chronic P. aeruginosa infections by antibiotics fails, phage therapy is a possibility to treat bacterial infections. (biomedcentral.com)
Sections on the prevention of bacterial pneumonia in mechanically ventilated and/or critically ill patients, care of respiratory-therapy devices, prevention of cross-contamination, and prevention of viral lower respiratory tract infections (e.g., respiratory syncytial virus {RSV} and influenza infections) have been expanded and updated. (cdc.gov)
After the Indian Ocean earthquake and tsunami in 2004, multidrug-resistant bacterial infections were often found in the survivors, and a tsunami-related tetanus epidemic was reported. (who.int)

image: Artist's rendition of a cross section of skin layers (stratum corneum, epidermis and dermis) shows topical application of an ionic liquid for combating a skin-borne bacterial infection. (eurekalert.org)
However, bacteria present in biofilms can evade the antibacterial effect, forming a reservoir of infection. (databasefootball.com)
The infecting organism may reach bone through blood or as a consequence of events such as trauma, surgery, the presence of foreign bodies, or the placement of prostheses that disrupt bony integrity and predispose to the onset of bone infection. (medscape.com)
When prosthetic joints are associated with infection, microorganisms typically grow in biofilm, which protects bacteria from antimicrobial treatment and the host immune response. (medscape.com)
E K Tsekoura et al, Battling bacterial infection with hexamethylene diisocyanate cross-linked and Cefaclor-loaded collagen scaffolds, Biomedical Materials (2017). (phys.org)
Besides this, bad breath, infection of the dental roots, and actinomycosis are also listed as some of the other concerns by these biofilms. (news-medical.net)
Due to this, many antibiotics become useless in the fight against this bacterium while the immune system also struggles to clear the bacterial infection. (ucalgary.ca)
By using a multidisciplinary approach, Harrison and his team learned more about how a strain of P. aeruginosa decides when to produce biofilm in an infection. (ucalgary.ca)
However, classic culture methods do not allow the simultaneous co-culture of different bacterial species, or the study in a more-realistic infection site environment, where several host factors are presented. (ibecbarcelona.eu)
As replication is a crucial step to initiate an infection, we have first focused on the study of the differential role of the different P. aeruginosa Ribonucleotide Reductase (RNR) enzymes in bacterial growth within the wound biofilm model, as RNR are essential enzymes in DNA replication. (ibecbarcelona.eu)
Also, we use a set of biofilm-degrading enzymes targeting the wound biofilm so as to improve the antibiotic delivery in the local area of the infection site. (ibecbarcelona.eu)
In addition to treating BV with hydrogen peroxide, you may consider taking a regular probiotic or eating probiotic foods to avoid further issues with bacterial infection. (earthclinic.com)
Phage therapy does have its limitations, however, including potential narrow host ranges, development of bacterial resistance to infection, and the potential spread of phage-encoded virulence genes. (cdc.gov)
Helicobacter pylori Infection Helicobacter pylori ( H. pylori ) infection is a bacterial infection that causes stomach inflammation ( gastritis), peptic ulcer disease, and certain types of stomach cancer. (msdmanuals.com)
Tularemia Tularemia is infection that is caused by the gram-negative bacteria Francisella tularensis , which is acquired when people have direct contact with infected wild animals, usually rabbits. (msdmanuals.com)
HAP is a common nosocomial bacterial infection and is most prevalent in medical and surgical intensive care units (ICUs). (medscape.com)

Microbes form a biofilm in response to a number of different factors, which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics. (wikipedia.org)
The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
Retrieved bacteria were genotyped, and grown in planktonic cultures and as biofilms, and susceptibilities to individual antibiotics and to antibiotic combinations were determined. (ersjournals.com)
Susceptibility to single antibiotics and to antibiotic combinations were not different between planktonically or biofilm-grown bacteria derived from sputum, as compared to those obtained by bronchoalveolar lavage and protected brush. (ersjournals.com)
However, studies have not previously been done to determine whether bacteria retrieved from sputum are more susceptible to antibiotics compared to bacteria retrieved directly from the airway biofilm. (ersjournals.com)
When bacteria team up in sticky communities called biofilms, they can be nearly impossible for conventional antibiotics to eradicate. (technologyreview.com)
Because they are protected by a sticky carbohydrate scaffold called a matrix, bacteria living communally in biofilms are a thousand times as resistant to antibiotics as free--swimming bacteria are, says Collins. (technologyreview.com)
What's more, some evidence suggests that the use of antibiotics actually induces biofilm formation. (technologyreview.com)
If the bacterial biofilm can be disrupted, delivery of antibiotics is greatly enhanced, and any dispersed pathogens are generally restored to normal antibiotic susceptibility," said Fox. (eurekalert.org)
Subsequent to the discovery of antibiotics during the last century, it was believed that these drugs would allow humans to conquer bacterial disease. (databasefootball.com)
The most common strategies employed by bacteria for resisting antibiotics include growing as biofilms, persisters, and small colony variants [1]. (databasefootball.com)
The resistance of biofilm-growing bacteria to antibiotics does not involve any genetic changes but is only a phenotypic adaptation. (databasefootball.com)
This mechanism renders the lysins highly bactericidal, and their rates of bacterial killing are far superior to those of conventional antibiotics. (databasefootball.com)
The poorly metabolizing persisters in the biofilms can thus evade the action of these antibiotics. (databasefootball.com)
Nanocrystaline silver apparently improves vulnerability of biofilms to antibiotics than larger silver particles because the small particles expose more surface. (healthjockey.com)
Microbes such as bacteria tend to live in complex colonies called biofilms, where they can resist antibiotics and cause more problems for the immune system. (sciencedaily.com)
Indeed resistance against antibiotics is itself one of the biggest problems of all associated with biofilms, Battin noted. (sciencedaily.com)
As the biofilms are resistant to antibiotics and the immune system's white blood cells, very often the only remedy is surgery, to replace a damaged valve, which can itself cause problems. (sciencedaily.com)
These biofilms exhibited decreased susceptibility to antibiotics and were adherent to epithelial surfaces. (nih.gov)
NTHi formed adherent biofilms on the apical surface airway epithelia with decreased susceptibility to antibiotics, and respiratory cells exhibited inflammatory and host defense responses-evidence of a dynamic host-pathogen interaction. (nih.gov)
The features displayed by the biofilm hinder and delay the healing processes, as such bacterial communities exhibit higher resistance to antibiotics and higher ability to evade the immune response. (ibecbarcelona.eu)
Another important characteristics of NGR are so called «quorum sensing» - the mechanism that controls the production of many factors of pathogenicity, and ability to form biofilm, the structure and physiological nature of which provide decreased susceptibility to antibiotics, antiseptics, and to immune system. (cmac-journal.ru)

Pseudomonas aeruginosa is the most common pathogen that chronically infects patients with cystic fibrosis (CF) 1 . (ersjournals.com)
Investigating alternative materials to EPDM for automatic taps in the context of Pseudomonas aeruginosa and biofilm control. (his.org.uk)
In cystic fibrosis, excess mucus production in the airways gives sanctuary to bacteria such as Pseudomonas aeruginosa, which actually mop up the dead carcasses of white blood cells sent by the immune system, enabling them to construct their protective biofilm coat. (sciencedaily.com)
This in vitro multispecies biofilm model resembles the natural conditions present in wounds and allows us to study a Pseudomonas aeruginosa and Staphylococcus aureus co-culture, which are the predominant bacteria found in wounds. (ibecbarcelona.eu)
The efficacy of low-temperature plasma torch was examined on bacterial species such as Pseudomonas aeruginosa and Staphylococcus aureus. (healthjockey.com)
The Pseudomonas infecting PB1-like phages are widespread in nature and possess highly conserved genomes. (biomedcentral.com)
Biofilms containing Pseudomonas aeruginosa, Micrococcus luteus, Stenotrophomonas maltophilia, Elizabethkingia anophelis, Cupriavidus metallidurans, and Methylobacterium fujisawaense were established in the CBR p-trap model for a period of 28 d. (cdc.gov)

Through a robust screening strategy, our research team has identified a unique class of materials, known as ionic liquids, which both neutralize biofilm-forming pathogens and deliver drugs through the skin," he said. (eurekalert.org)
The comprehensive strategy resulted in the identification of ionic liquids that are effective at disrupting biofilms, neutralizing pathogens, and enhancing delivery of antibiotic into skin. (eurekalert.org)
The urinary microbiota is similarly complex as the vaginal and penile microbiota, yet its role as a reservoir for pathogens and for recurrent polymicrobial biofilm diseases like bacterial vaginosis (BV) is not clear. (biomedcentral.com)
Biofilms formed by these health care-associated pathogens can lead to negative and costly health outcomes. (cdc.gov)
Sometimes, under certain conditions, the resident bacterial flora can act as pathogens and cause disease. (msdmanuals.com)
however, more recent studies have indicated that many individuals who met the criteria for HCAP were not infected with MDR pathogens. (medscape.com)

Clin Infect Dis 2004;38(11):1586-91. (cdc.gov)
Clin Infect Dis 2005;41(1):77-82. (cdc.gov)
Clin Infect Dis 2003;37(8):1094-101. (cdc.gov)
Clin Infect Dis 2000;31(2):590-6. (cdc.gov)

Further, many bacterial infestations in wounds penetrate under the outer skin layer, the stratum corneum, and deep into the tissue (epidermis and dermis). (eurekalert.org)
Biofilms are a major cause of chronic wounds and wound degeneration. (eurekalert.org)
Wounds from infected surgical incisions result in 1 million additional hospital days. (eurekalert.org)
Additional causes of bacterial infected wounds include traumatic injuries, as well as diabetic foot ulcers, venous leg ulcers, and pressure ulcers. (eurekalert.org)
Healing wounds may be difficult as the bacterial biofilms frequently thrive in injuries. (healthjockey.com)
Researchers have now identified a better method of using silver to treat infected wounds. (healthjockey.com)
Resistant to antibiotic treatment, biofilm not only stalls the healing process of chronic wounds but also puts patients at greater risk for amputation. (woundsource.com)
The treatment of acute and chronic infected wounds with residing biofilm still poses a major challenge in medical care. (lohmann-rauscher.com)
This analysis aimed to extend the investigation on the efficacy of a variety of antimicrobial dressings using an in vitro biofilm model (lhBIOM) mimicking the specific biofilm-environment in human wounds. (lohmann-rauscher.com)
Bacteria infecting wounds arrange themselves in polymicrobial communities known as biofilms. (ibecbarcelona.eu)
On an average, the plasma was apparently able to kill 90 percent of the bacteria infecting skin wounds in rats after ten minutes. (healthjockey.com)
People can become infected through open wounds or implanted devices, like heart valves and artificial hips. (harvard.edu)

Biofilms comprising millions of bacteria are at the root of many serious chronic infectious diseases such as cystic fibrosis and periodontal disease, as well as industrial contamination, biofouling and biocorrosion. (sciencedaily.com)
Nontypeable Haemophilus influenzae (NTHi) commonly infects patients with cystic fibrosis (CF), especially early in childhood. (nih.gov)

Dressing performance was tested against P. aeruginosa biofilms over the course of 6 days. (lohmann-rauscher.com)
As such, P. aeruginosa biofilms are major burdens in hospital settings. (ucalgary.ca)

Microorganisms attach to surfaces and develop biofilms. (cdc.gov)
Rediscovery of a microbiologic phenomenon, first described by van Leeuwenhoek, that microorganisms attach to and grow universally on exposed surfaces led to studies that revealed surface-associated microorganisms (biofilms) exhibited a distinct phenotype with respect to gene transcription and growth rate. (cdc.gov)
These biofilm microorganisms have been shown to elicit specific mechanisms for initial attachment to a surface, development of a community structure and ecosystem, and detachment. (cdc.gov)
Van Leeuwenhoek, using his simple microscopes, first observed microorganisms on tooth surfaces and can be credited with the discovery of microbial biofilms. (cdc.gov)
Heukelekian and Heller ( 1 ) observed the "bottle effect" for marine microorganisms, i.e., bacterial growth and activity were substantially enhanced by the incorporation of a surface to which these organisms could attach. (cdc.gov)
5 ) in 1978 put forth a theory of biofilms that explained the mechanisms whereby microorganisms adhere to living and nonliving materials and the benefits accrued by this ecologic niche. (cdc.gov)
A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
Besides s treptococci, various other microorganisms can also form biofilms at varied sites. (news-medical.net)
Bacteriophages have reemerged as potential biocontrol agents, particularly against biofilm-associated, drug-resistant microorganisms. (cdc.gov)

Although bone is normally resistant to bacterial colonization, it can become infected in multiple ways. (medscape.com)
Microscopic evaluation of these biofilms indicated that streptococcus accounts for approximately 80 percent of this colonization. (news-medical.net)
V. cholerae also forms biofilms upon colonization on their copepod symbionts. (kenyon.edu)
This study aimed to determine the effect of LW on biofilm formation and CPKP colonization in a P-Trap model (PTM). (cdc.gov)
The primary objective of our study was to formulate a phage cocktail capable of targeting a CPKP strain (CAV1016) at different stages of colonization within polymicrobial drinking water biofilms using a CDC biofilm reactor (CBR) p-trap model. (cdc.gov)

The strains were allowed to form biofilm in vitro. (jmb.or.kr)
Growth pattern of Mycobacterium species are different such as M. chelonae and M. fortuitum form biofilm as vertical and entire surface growth, respectively [ 93 ]. (biomedcentral.com)

High throughput sequencing was performed to identify the genes involved in biofilm formation and to postulate the mechanism of action. (biomedcentral.com)
To date there have been a limited number of studies on the transcriptome of bacteria and the genes involved in biofilm formation. (biomedcentral.com)

Treatment of lapine knees infected with Staphylococcus aureus with the antibiotic-eluting UHMWPE led to complete bacterial eradication and the absence of detectable systemic effects. (nature.com)
Treatment of established biofilm with NEBB resulted in biofilm-disruption, involving significant reductions in biofilm mass and metabolic activity for Candida and both Staphylococcus species. (jmb.or.kr)

Antimicrobial proteins and peptides offer a multifaceted mechanism suitable to fight bacterial resistance. (frontiersin.org)
Bacteriophage are unaffected by antibiotic resistance and are able to disrupt bacterial biofilms. (businesswire.com)
Such biofilms are a major line of defense for bacteria, contributing to antibiotic resistance. (businesswire.com)
For example, factors promoted by S aureus may promote bacterial adherence, resistance to host defense mechanisms, and proteolytic activity. (medscape.com)
However, thicker biofilms supposedly have some resistance to treatment. (healthjockey.com)
The phenotype of S . Typhi biofilm cells is significantly different from the free-swimming planktonic cells, and studies have shown that they are associated with antibiotic resistance, immune system evasion, and bacterial persistence. (biomedcentral.com)
The results showed that the genes associated with metabolic processes and biofilm regulations were down-regulated while those associated with the membrane matrix and antibiotic resistance were highly up-regulated. (biomedcentral.com)
While in the gallbladder, the bacteria forms a biofilm on either the gallstones or gallbladder epithelium, providing a stable environment for growth and survival of the bacteria, and resulting in increased resistance towards the host's immune system and antimicrobial agents [ 7 , 8 ]. (biomedcentral.com)
E. faecalis has demonstrated a high resistance 20 and ability to inactivate antimicrobial agents 14 , survival capacity in harsh environments, with scarce nutrient supply and extreme alkaline pH 28 , and the capacity for growth as a biofilm on root canal walls 21 . (bvsalud.org)
The global spread of antibiotic resistance, coupled with increased tolerance to antimicrobial treatments in biofilm-associated bacteria, highlights the need for novel strategies to overcome treatment hurdles. (cdc.gov)

The recalcitrance of bacteria in biofilms has been attributed to the impermeable nature of the biofilm matrix and to the presence of non-growing or very slowly-growing populations of bacteria called persisters. (databasefootball.com)
This gives the slimy nature of the biofilm, and contains many sugars such as fructose, glucose, mannose, rhamnose, galactose and N-acetylglucosamine. (thepigsite.com)

Two major thrusts in the last decade have dramatically impacted our understanding of biofilms: the utilization of the confocal laser scanning microscope to characterize biofilm ultrastructure, and an investigation of the genes involved in cell adhesion and biofilm formation. (cdc.gov)
Formation of a biofilm starts with adherence of bacteria followed by production of the polysaccharide matrix. (thepigsite.com)
Control of biofilms is difficult and addresses preventing initial formation and removing existing biofilms. (thepigsite.com)
The formation of a biofilm is not a random process. (thepigsite.com)
In bronchoalveolar lavage fluid samples from young, asymptomatic patients with CF, we found morphologic evidence suggestive of NTHi biofilm formation. (nih.gov)
They can require long hospitalisation periods to disturb and treat bacterial biofilm formation. (phys.org)
Pilin expression is associated with auto-agglutination in liquid suspension or broth cultures, and appears to be necessary but not sufficient for expression of the agar-pitting phenotype and for the formation of biofilms. (microbiologyresearch.org)
Common plant flavonoids prevent the assembly of amyloid curli fibres and can interfere with bacterial biofilm formation. (hu-berlin.de)
Kettles, R., N. Tschowri, K. Lyons, P. Sharma, R. Hengge, M. Webber, and D. Grainger (2019) The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation. (hu-berlin.de)
Midha, A., K. Janek, A. Niewienda, P. Henklein, S. Guenther, D.O. Serra, J. Schlosser, R. Hengge, and S. Hartmann (2018) The intestinal roundworm Ascaris suum releases antimicrobial factors which interfere with bacterial growth and biofilm formation. (hu-berlin.de)
However, the mechanism of this transition and the events leading to biofilm formation are unknown. (biomedcentral.com)
This data provide a basis for further studies to uncover the mechanism of biofilm formation in S . Typhi and to discover novel genes or pathways associated with the development of the typhoid carrier state. (biomedcentral.com)
Biofilm formation has been found in 95% of patients intubated with endo-tracheal tubes for more than 24h. (ibecbarcelona.eu)
Secondary colonising bacteria then adhere to the primary colonisers resulting in biofilm formation. (springer.com)
Quorum sensing plays an important role in regulating the biofilm formation. (biomedcentral.com)
iii) colony formation and biofilm maturation. (biomedcentral.com)
Metabolic activity of the bacterial biofilm communities have altered as compared to the planktonic one such as increased rates of EPS production, activation or inhibition of particular genes associated with biofilm formation and decreased growth rate [ 39 ]. (biomedcentral.com)
Formation of biofilms in mycobacteria can be defined as similar as other biofilms communities. (biomedcentral.com)
Panels of studies have reported the role of various molecules in the biofilm formation and maintenance of its composition. (biomedcentral.com)
Glycopeptidolipids are indispensable for initial surface attachment during biofilm formation in Mycobacterium smegmatis [ 111 ]. (biomedcentral.com)
Biofilm formation over the dentin surface was ensured by SEM analysis. (bvsalud.org)
Maintenance of the water system is also necessary in order to reduce biofilm formation and Legionella recolonization. (who.int)

These attached communities are known as biofilms. (thepigsite.com)

Working with Boston University synthetic biologist James Collins, -Harvard-MIT graduate student Timothy Lu has designed a new, highly effective means of dispersing and killing the bacteria living in biofilms. (technologyreview.com)

The vast majority actually live in highly organized communities called biofilms, which can contain many different species. (harvard.edu)

The ESF workshop also highlighted greater understanding of the complex interactions within biofilms, which often comprise not just one species of bacteria, but a whole host of different micro-organisms, including archaea, protozoa, fungi, and even tiny metazoa actually comprising multiple cells. (sciencedaily.com)
Over 700 bacterial species co-inhabit healthy human mouth. (news-medical.net)
Microbial forms living in biofilms are morphologically and functionally distinct from those of free-floating (planktonic) forms of the same species. (jmb.or.kr)
This new solution is capable of detecting a number of infectious bacterial species, and organisms normally present on human skin don't trigger the color change. (springwise.com)
Moreover, in mixed-species biofilms, the waste products of one type of bacteria can be used by another species. (harvard.edu)
However, many different bacterial species may cause PJIs and there has previously been no nationwide assessment of bacteria causing revisions of THAs in Norway. (openorthopaedicsjournal.com)

3 weeks) S. aureus was the most frequent bacterial finding. (openorthopaedicsjournal.com)

PTMs containing polymicrobial biofilms grown in autoclaved municipal tap water (ATW) supplemented with 5% dextrose in water (D5W), nutritional shake (Shake), sugar-based soft drink (Soda), or ATW were inoculated with K. pneumoniae ST258 KPC+ (ST258) or K. pneumoniae CAV1016 (CAV1016) and sampled after 7, 14, and 21 d. (cdc.gov)

The new technique will allow researchers to build bacteriophage cocktails for attacking all kinds of biofilms. (technologyreview.com)

Infect Control Hosp Epidemiol 1996;17(1):53-80. (cdc.gov)
Infect Control Hosp Epidemiol 2004;25(12):1020-5. (cdc.gov)
Infect Control Hosp Epidemiol 1998;19(6):407-63. (cdc.gov)
Infect Control Hosp Epidemiol 2003;24(5 Suppl):S6-52. (cdc.gov)

Biofilms may form on living (biotic) or non-living (abiotic) surfaces and can be prevalent in natural, industrial, and hospital settings. (wikipedia.org)
However, on solid surfaces, bacterial cells tend to grow together in a matrix secreted by the cells. (databasefootball.com)
The silver atoms on those surfaces supposedly interact with macromolecules like DNA and proteins within the biofilms. (healthjockey.com)
In addition, 10 clinical NTHi isolates from patients with CF formed biofilms on plastic surfaces. (nih.gov)
Soon after establishing multilayered biofilms streptococci establish adhesions on their surfaces. (news-medical.net)
It is proposed that the biofilm phenotype of S . Typhi allows the bacteria to increase production of the membrane matrix in order to serve as a physical shield and to adhere to surfaces, and enter an energy conservation state in response to the stressful environment. (biomedcentral.com)
Bacterial films can form on nearly any surface that is in contact with water: the insides of pipes, the outsides of boats, and even the surfaces of your teeth and the lining of your intestines. (harvard.edu)
On the surfaces of water pipes, these biofilms can be a serious obstacle to producing clean drinking water. (harvard.edu)
Biofilms are a major suspect in such cases, especially since they are resilient enough to live for months on gloves, floors, surgical instruments, and other hospital surfaces. (harvard.edu)
Recent studies have demonstrated biofilm growth over root surfaces on teeth with chronic apical periodontitis and teeth refractory to root canal treatment 12 . (bvsalud.org)

Importantly, bacteria are capable of forming biofilms in infected human tissues. (databasefootball.com)

The results suggest that targeted nutraceutical support may help disrupt biofilm communities, offering new facets for integrative combinational treatment strategies. (jmb.or.kr)

We use a novel in vitro culture approach, optimizing a method to assess bacterial viability in a wound biofilm model. (ibecbarcelona.eu)

The present authors hypothesised that bronchoscopy with protected specimen brush may sample biofilm-forming bacteria adherent to the airway wall, whereas traditional sputum collection may not. (ersjournals.com)
Recent evidence suggests that P. aeruginosa may actually grow as dense communities of exopolysaccharide-encapsulated biofilms adherent to CF patients' airways 2 - 4 . (ersjournals.com)
A microbial biofilm is a community of adherent microbial cellular forms with properties that help protect the microbial community from disruption by physical, chemical, or immunological attack. (jmb.or.kr)

Bacteria biofilms are increasingly recognized as contributing to bacterial persistence and disease pathogenesis in CF. (nih.gov)

This has excellent prospects for aiding antibiotic delivery to the pathogen through biofilm disruption but, most interestingly, the ionic liquids themselves are quite effective for pathogen neutralization," Fox said. (eurekalert.org)
As it is caused by this aquatic pathogen, cholera is most commonly spread by water supplies contaminated with stools infected with V. cholerae . (kenyon.edu)
Bartonella henselae is a fastidious, Gram-negative bacterial pathogen of cats and humans. (microbiologyresearch.org)

J Hosp Infect. (his.org.uk)
Kümin M , Jones CI , Woods A, Bremner S , Reed M, Scarborough M and Harper CM. Resistant fabric warming is a viable alternative to forced-air warming to prevent inadvertent perioperative hypothermia during hemiarthroplasty in the elderly J Hosp Infect. (his.org.uk)
Lynch C , Mahida N, Oppenheim B and Gray J. Washing our hands of the problem J Hosp Infect 2020 Oct: 104 (4): 401 - 403. (his.org.uk)
J Hosp Infect 2020 July 105 (3), 585 - 586. (his.org.uk)

Klauck, G., D.O. Serra, A. Possling, and R. Hengge (2018) Spatial organisation of different sigma factor activities and c-di-GMP signalling within the 3D landscape of a bacterial biofilm. (hu-berlin.de)
1) Galperin, M.Y. and D.N. Shalaeva (2018) A bacterial coat that is not pure cotton. (hu-berlin.de)

A biofilm usually begins to form when a free-swimming bacterium attaches to a surface. (wikipedia.org)
Bacterium forming a biofilm as heat increases. (ucalgary.ca)
In this case, the bacterium needs to change its virulence, which is its ability to efficiently infect the host. (ucalgary.ca)

The viruses they work with, called bacteriophages, specialize in infecting bacteria. (technologyreview.com)
Lysins are enzymes derived from bacteriophages (viruses which can kill bacteria) which can cleave bacterial peptidoglycan, a unique covering present in the cell wall of bacteria. (databasefootball.com)
Bacteriophages (phages), or viruses that infect bacteria, have reemerged as one such potential strategy. (cdc.gov)

Cells in a bacterial colony move in confined space surrounded by boundaries between gas, liquid, and solid phases. (nature.com)
This initial colony grows by reproducing to form daughter cells and trapping other microbes that become part of the biofilm. (harvard.edu)
In addition, the diversity of bacteria in a colony can make it difficult for a single treatment to destroy the biofilm. (harvard.edu)

Bacterial colonies with active TdcA proteins looked completely different depending on different temperatures. (ucalgary.ca)
Our findings present a unique form of bacterial self-organization that influences population structure and material distribution in colonies. (nature.com)
For example, in many occasions bacterial colonies are sessile and their expansion is driven by growth rather than by cell motility, but these sessile colonies normally preserve a subpopulation of motile cells for reasons that are not well understood 17 . (nature.com)
This type of bacterial self-organization has not yet been observed in the context of naturally developed colonies. (nature.com)
Here we sought to examine the behavior of motile cell populations in bacterial colonies and to explore their potential physiological functions. (nature.com)

The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. (wikipedia.org)
Sequencing libraries were prepared from S . Typhi planktonic cells and mature biofilm cells using the Illumina HiSeq 2500 platform, and the transcriptome data obtained were processed using Cufflinks bioinformatics suite of programs to investigate differential gene expression between the two phenotypes. (biomedcentral.com)

Most of the infecting microbes are killed by stomach acid. (kenyon.edu)

It has been hypothesized that the transformation of this organism from the planktonic to the biofilm phenotype leads to persistence and development of the carrier state in man. (biomedcentral.com)

In a groundbreaking manuscript appearing this week in the Proceedings of the National Academy of Science, as part of a multi-institutional effort between Los Alamos, University of California Santa Barbara, Dixie State University and Northern Arizona University, researchers explored exploiting ionic liquids both in a concerted effort to combat antibiotic-resistant bacterial biofilms in skin, as well as for topical transdermal drug delivery. (eurekalert.org)
Thus, an ideal new antibacterial agent, in addition to its activity on drug-resistant strains of bacteria, should also be capable of killing bacteria in biofilms. (databasefootball.com)
Microbial biofilms are resilient, immune-evasive, often antibiotic-resistant health challenges, and increasingly the target for research into novel therapeutic strategies. (jmb.or.kr)
For B. burgdorferi , we observed reduced biofilm mass, but the remaining residual biofilm showed a mild increase in metabolic activity, suggesting a shift from metabolically quiescent, treatment-resistant persister forms of B. burgdorferi to a more active form, potentially more recognizable by the host immune system. (jmb.or.kr)

The post-treatment biofilm mass was evaluated by crystal-violet staining, and metabolic activity was measured using the MTT assay. (jmb.or.kr)
Average biofilm mass and metabolic activity for NEBB-treated biofilms were compared to the average of untreated control cultures. (jmb.or.kr)
For P. aeruginosa , low doses of NEBB significantly reduced biofilm mass and metabolic activity while higher doses of NEBB increased biofilm mass and metabolic activity. (jmb.or.kr)

If such bacteria-attacking viruses prove safe for industrial and clinical use, researchers could develop stocks of different kinds of viruses, each tailored to attack a different kind of biofilm. (technologyreview.com)
In clinical settings, biofilms are often found growing on various medical devices such as catheters implanted in patients. (databasefootball.com)
En mayo de 2007 obtuve el Certificate of Training in Molecular Biological Techniques en el Department of Molecular Biology y desde Junio de 2008 a Junio de 2009 completé el Certificate in Clinical Research en el Center for Translational Science Activities en Mayo Clinic College of Medicine, Rochester Minnesota. (unav.edu)
Un 40% de esos trabajos han sido publicados en revistas del primer cuartil entre las que destacan New England Journal of Medicine, Lancet, Lancet Infectious Diseases, Clinical Infectious Diseases, Clinical Microbiology Reviews, Antimicrobial Agents and Chemotherapy, Journal Clinical Microbiology, Journal Antimicrobial Chemotherapy, Mayo Clinic Proceedings y Malaria Journal. (unav.edu)
We isolated and characterized a phage, named JG024, which infects a broad range of clinical and environmental P. aeruginosa strains. (biomedcentral.com)
One single observer visited ten representative hospitals where clinical history, preoperative blood samples and the bacterial findings of intraoperative samples were collected. (openorthopaedicsjournal.com)

Endocarditis is a rare but serious disease in which one of the four heart valves, the heart lining, or heart muscle, are infected by a bacterial biofilm, often comprising streptococci, and become inflamed. (sciencedaily.com)
For example, bacterial endocarditis is one such condition that can affect the human heart. (news-medical.net)

An established biofilm structure comprises microbial cells and EPS, has a defined architecture, and provides an optimal environment for the exchange of genetic material between cells. (cdc.gov)

The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides, proteins, lipids and DNA. (wikipedia.org)
This molecule can bind to proteins and regulate the swimming behaviour of bacteria and the production of biofilm. (ucalgary.ca)
Biofilm cultures were treated with NEBB containing enzymes targeted at lipids, proteins, and sugars, also containing the mucolytic compound N-acetyl cysteine, along with antimicrobial extracts from cranberry, berberine, rosemary, and peppermint. (jmb.or.kr)

Cells may also communicate via quorum sensing, which may in turn affect biofilm processes such as detachment. (cdc.gov)
Bacteria within a biofilm communicate by quorum sensing leading to phenotypic modifications and changes in gene expression. (thepigsite.com)
Quorum sensing is a type of bacterial communication that occurs via the secretion of autoinducer molecules (5). (kenyon.edu)

How Can I Naturally Treat Bacterial Vaginosis? (earthclinic.com)

Biofilms present in the oral cavity are essential for the sustenance of good oral health but an increase in the volume and complexities of these biofilms can result in diseases like gingivitis and Periodontitis. (news-medical.net)

This document updates and replaces CDC's previously published 'Guideline for Prevention of Nosocomial Pneumonia' (Infect Control 1982;3:327-33, Respir Care 1983;28:221-32, and Am J Infect Control 1983;11:230-44). (cdc.gov)

Biofilm-associated cells can be differentiated from their suspended counterparts by generation of an extracellular polymeric substance (EPS) matrix, reduced growth rates, and the up- and down- regulation of specific genes. (cdc.gov)
A biofilm is an assemblage of surface-associated microbial cells that is enclosed in an extracellular polymeric substance matrix. (cdc.gov)
By using a specific polysaccharide-stain called Ruthenium red and coupling this with osmium tetroxide fixative, these researchers were also able to show that the matrix material surrounding and enclosing cells in these biofilms was polysaccharide. (cdc.gov)
Subpopulations of cells within the biofilm differentiate to perform various activities for motility, matrix production, and sporulation, supporting the overall success of the biofilm. (wikipedia.org)
They can be found very early in Earth's fossil records (about 3.25 billion years ago) as both Archaea and Bacteria, and commonly protect prokaryotic cells by providing them with homeostasis, encouraging the development of complex interactions between the cells in the biofilm. (wikipedia.org)
it even provides something like a primitive circulatory system, distributing nutrients to cells throughout the biofilm. (technologyreview.com)
and then spread to other bacterial cells. (technologyreview.com)
Yet there is also great excitement about an emerging application that could have some potential for green energy production - the use of biofilms to power microbial fuel cells whose fuel could be wastewater, as outlined at the ESF workshop by Cristian Picioreanu, Delft University of Technology. (sciencedaily.com)
Planktonic S . Typhi cells were cultured using standard nutrient broth whereas biofilm cells were cultured in a stressful environment using high shearing-force and bile to mimic the gallbladder. (biomedcentral.com)
Instead, the cells take on different roles, including the initial free-swimming bacteria, the scaffolding-producing cells, and, eventually, a third type of cell that send out spores to start new biofilms. (harvard.edu)
For instance, Professor Kolter's lab at Harvard is studying how the cells switch between their various roles in different parts of the biofilm. (harvard.edu)

In this work, we have tested the human canonical members of the RNase family using a spot-culture growth inhibition assay based mycobacteria-infected macrophage model for evaluating their anti-tubercular properties. (frontiersin.org)

A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated. (wikipedia.org)
Adaptation of E. coli biofilm growth, morphology and mechanical properties to substrate water content. (hu-berlin.de)
In many older oil wells, much of the liquid extracted is water, which makes the insides of pipelines an ideal growth medium for biofilms. (harvard.edu)
Bacterial growth in two or more samples was found in 278 revisions, and thus included. (openorthopaedicsjournal.com)
Biofilms have been found to be one of the most common growth conditions for bacteria in nature. (bvsalud.org)

Osteomyelitis is inflammation of the bone caused by an infecting organism. (medscape.com)

Because intubation and mechanical ventilation alter first-line patient defenses, they greatly increase the risk for nosocomial bacterial pneumonia. (cdc.gov)

Bacteriophage are able to penetrate biofilms and replicate locally to high levels, to produce strong local therapeutic effects. (businesswire.com)

Therefore, phages and especially the ability of the phage to infect the host in vivo should be investigated carefully prior to use. (biomedcentral.com)
We used an artificial sputum medium to simulate the conditions in the CF lung and investigated the ability of phage JG024 to infect P. aeruginosa and multiply under these conditions. (biomedcentral.com)
That being said, advances in phage isolation, screening, and genome sequencing tools provide an upside in overcoming some of these limitations and open up the possibilities of using phages as effective biofilm control agents. (cdc.gov)
Biofilms were treated for 2 h at either 25 C or 37 C with the phage cocktail (109 PFU/ml) at 7, 14, and 21 d post-inoculation. (cdc.gov)
The effect of phage treatment on the viability of biofilm-associated CAV1016 was determined by plate count on m-Endo LES agar. (cdc.gov)
Supplementation with a non-ionic surfactant appears to enhance phage association within biofilms. (cdc.gov)

Organisms8

Chemicals and Drugs5

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes7

Technology, Industry, Agriculture1

Health Care1

Infections33

Infection16

Antibiotics22

Pseudomonas7

Pathogens6

Clin Infect Dis4

Wounds12

Cystic fibrosis2

Aeruginosa biofilms2

Microorganisms11

Colonization5

Form biofilm2

Involved in biofilm formation2

Staphylococcus2

Resistance10

Nature of the biofilm2

Formation22

Known as biofilms1

Bacteria living in b1

Communities called biofilms1

Species6

Aureus1

Polymicrobial1

Kinds of biofilms1

Control Hosp Epidemiol4

Surfaces10

Human tissues1

Disrupt1

Wound Biofilm1

Adherent3

Pathogenesis1

Pathogen3

Hosp Infect4

20182

Bacterium3

Bacteriophages3

Colony3

Colonies5

Planktonic cells2

Microbes1

Phenotype1

Resistant4

Metabolic3

Clinical6

Endocarditis2

Comprises1

Proteins3

Quorum3

Treat bacterial1

Periodontitis1

Nosocomial pneumonia1

Cells11

Assay1

Growth5

Organism1

Pneumonia1

Replicate1

Phage6