One-step growth curve experiments revealed that the phages have latent periods of 50-80 min with burst sizes of 44-65 virions per infected cell. (mdpi.com)
The results showed that both phages could infect many serotypes of E. coli. (cdc.gov)
Bacteriophages (phages) are viruses capable of infecting and replicating within bacterial cells. (asm.org)
Virulent or obligate lytic phages infect and quickly kill their bacterial host cell, whereas temperate or lysogenic phages may either stably integrate into their host's genome or enter into the lytic life cycle. (asm.org)
primarily Bacteroides phages and specific sero/genogroups of F-specific RNA coliphages. (bluephage.com)
One of the densest natural sources for phages and other viruses is sea water, where up to 9×10 8 virions per milliliter have been found in microbial mats at the surface [2] , and up to 70% of marine bacteria may be infected by phages. (wikidoc.org)
The scientists were doubly surprised because the phages it defeated in testing all infect using double-stranded DNA, but the CRISPR-Cas13 system only targets and chops single-stranded viral RNA. (sciencedaily.com)
The CRISPR-Cas13 system effectively neutralized nine different DNA phages that all infect strains of E. coli, yet have almost no similarity across their genomes. (sciencedaily.com)
micromicrobe: " Microbial image of the month I've previously posted an image of a single phage, but this month's image shows phages in action, infecting a bacterial cell. (scoop.it)
The Pseudomonas infecting PB1-like phages are widespread in nature and possess highly conserved genomes. (biomedcentral.com)
Therefore, phages and especially the ability of the phage to infect the host in vivo should be investigated carefully prior to use. (biomedcentral.com)
Analysis of CRISPR spacers indicates that phages of all three putative families infect bacteria of the phylum Bacteroidetes. (biomedcentral.com)
When the phages infected the DNA-laden microbes, a small percentage of the phages reproducing inside the microbes took up the altered DNA and incorporated it into their genomes in place of the original sequence. (globalhealthnewswire.com)
coliphage and Escherichia coli titers and enteropathogens were determined in stool and quantitative diarrhea parameters (stool output, stool frequency) were measured. (pacb.com)
Scholars@Duke publication: Nucleotide sequence of a gene cluster involved in entry of E colicins and single-stranded DNA of infecting filamentous bacteriophages into Escherichia coli. (duke.edu)
Mutations in fii or tolA of the fii-tolA-tolB gene cluster at 17 min on the Escherichia coli map render cells tolerant to high concentrations of the E colicins and do not allow the DNA of infecting single-stranded filamentous bacteriophages to enter the bacterial cytoplasm. (duke.edu)
Bacteriophages4
The therapeutic use of bacteriophages, viruses that infect and kill bacteria, is well suited to be part of the multidimensional strategies to combat antibiotic resistance. (asm.org)
Microbiologists start searching for enteric bacteriophages other than somatic coliphages that do not replicate outside the digestive tract. (bluephage.com)
A team led by CRISPR pioneer Jennifer Doudna and her longtime collaborator Jill Banfield has developed a clever tool to edit the genomes of bacteria-infecting viruses called bacteriophages using a rare form of CRISPR. (sciencedaily.com)
Somatic coliphages6
Samples were evaluated for levels of fecal contamina- tion by using fecal coliforms and somatic coliphages. (cdc.gov)
Somatic coliphages were enumerated according to can also transduce resistance genes from Salmonella enter- the standard method. (cdc.gov)
They were mainly looking for somatic coliphages with strains and media quite diverse from one to another study. (bluephage.com)
Sufficient evidence emerged to claim that, if occurring, the potential effects of replication in the aquatic environment of somatic coliphages would not affect the numbers detected in water samples . (bluephage.com)
This led to the acceptance of somatic coliphages as indicators of fecal/viral contamination of waters. (bluephage.com)
Bacteriophage (phage) are ubiquitous viruses that infect and kill bacteria in a species-, sometimes even strain-, specific manner. (diabetesonthenet.com)
Bacterial2
Phage therapy (PT), the use of bacterial viruses infecting the pathogen in a species-specific way, is a potential alternative. (pacb.com)
Method T4-like coliphages or a commercial Russian coliphage product or placebo was orally given over 4 days to Bangladeshi children hospitalized with acute bacterial diarrhea. (pacb.com)
Bacteroides1
Two of the candidate families, "Flandersviridae" and "Quimbyviridae", include some of the most common and abundant members of the human gut virome that infect Bacteroides , Parabacteroides , and Prevotella . (biomedcentral.com)
Fecal2
Coliphage levels reflect the persistence of pathogenic viruses in the environment and have been proposed as an indicator of fecal contamination in water. (wikipedia.org)
Fecal coliphage was increased in treated over control children, but the titers did not show substantial intestinal phage replication (secondary microbiology outcome). (pacb.com)
Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. (nih.gov)
Interpretation Oral coliphages showed a safe gut transit in children, but failed to achieve intestinal amplification and to improve diarrhea outcome, possibly due to insufficient phage coverage and too low E. coli pathogen titers requiring higher oral phage doses. (pacb.com)
This decade contemplates the general acceptance of E. coli C as an appropriate host strains for somatic coliphage detection. (bluephage.com)
Pseudomonas4
Phage ϕIBB-PF7A is a T7-like bacteriophage capable of infecting several Pseudomonas fluorescens dairy isolates and is extremely efficient in lysing this bacterium even when growing in biofilms attached to surfaces. (biomedcentral.com)
Twenty eight (46.7%) of the proteins encoded by this virus exhibit sequence similarity to coliphage T7 proteins while 34 (81.0%) are similar to proteins of Pseudomonas phage gh-1. (biomedcentral.com)
That this phage is closely related to Pseudomonas putida phage gh-1 and coliphage T7 places it in the "T7-like viruses" genus of the subfamily Autographivirinae within the family Podoviridae . (biomedcentral.com)
In this manuscript we report on the full genomic characterization of Pseudomonas fluorescens phage ϕIBB-PF7A, a phage capable of infecting several dairy P. fluorescens isolates including isolates belonging to different ribotypes [ 9 ]. (biomedcentral.com)
Occurrence1
The analysis of coliphages was performed by plaque assay, while the occurrence of enteric viruses and crAssphage was determined by molecular techniques. (encyclopedia.pub)
Characterization1
Morphological, host range, and genetic characterization of two coliphages. (cdc.gov)
Species1
Viruses infect all kingdoms of life, and new species are continuously being discovered. (fortunejournals.com)
Strains1
We isolated and characterized a phage, named JG024, which infects a broad range of clinical and environmental P. aeruginosa strains. (biomedcentral.com)
Indicators3
It was not until the late 1970s and early 1980s that some very scattered papers were published that could be called pioneering in using coliphages as viral indicators. (bluephage.com)
Though not supported by sound pieces of evidence, the idea that coliphages multiply in water and therefore cannot be used as indicators emerged and began to be widely accepted. (bluephage.com)
Meanwhile, data continue to accumulate to ensure the suitability of coliphages as indicators of viral contamination: 1) their abundance in different local and geographic environments, and 2) their behavior against disinfection and natural inactivation. (bluephage.com)
Genes1
The genome of coliphage T7 has 56 genes encoding potential proteins [ 8 ], of which 35 have known function or functions. (biomedcentral.com)
Viral1
Purification of viral DNA from infected cells also indicated that 5- 3 H-uridine was associated with viral nucleic acid possibly as ribonucleotides. (microbiologyresearch.org)
Humans1
Coliphages do not infect humans or cause illness. (cdc.gov)
Enteric1
Accumulation of crAssphage and coliphages was observed in PW, but correlation with human enteric viruses was not possible due to the low prevalence of these pathogens in the PW analyzed. (encyclopedia.pub)
Multiply1
We used an artificial sputum medium to simulate the conditions in the CF lung and investigated the ability of phage JG024 to infect P. aeruginosa and multiply under these conditions. (biomedcentral.com)
Virions1
When time's up, an infecting phage lyses its host cell, thus releasing the assembled virions. (asmblog.org)
Virus1
If you are tested for COVID-19, and the test comes back positive, you can be very sure that you are infected with this virus. (limelightexperience.com)
Resistance1
It is a period for many publications about sensitivity/resistance to disinfection done with reference coliphages such as f2, MS2, T4, and contribution of mounting data on the presence of coliphages in different water matrixes. (bluephage.com)
Plaque1
In terms of growth cycle, phage ϕIBB-PF7A has a latent period of 15 minutes, an eclipse period of 10 minutes and a burst size of 153 plaque forming units per infected cell. (biomedcentral.com)
Genetic1
When someone is infected, they have this genetic material in their nose and upper throat. (limelightexperience.com)
Cells2
The animal viruses infect the animal cells and cause different fatal diseases in animals including animals. (zoologytalks.com)
Moreover, ϕIBB-PF7A is the first reported phage capable of efficiently infecting elongated P. fluorescens cells - which are up to 10 times longer than normal sized cells - and killing planktonic stationary (3 days old) phase cells [ 9 ]. (biomedcentral.com)
Water1
Coliphages as tools in microbiological water quality management: 60 years of research development. (bluephage.com)
Positive1
Fortunately, these cases could still be recognized as infected by No matter the method, all SARS-CoV-2 tests are reported qualitatively (positive/negative, etc). (limelightexperience.com)
London1
In 1915, British bacteriologist Frederick Twort , superintendent of the Brown Institution of London, discovered a small agent that infected and killed bacteria. (wikidoc.org)