• In previous studies, induced pluripotent stem cells (iPSCs) differentiated into imDCs, and sinomenine (SN) was used to inhibit the maturation of imDCs. (newswise.com)
  • The effects of iPSCs-imDCs and SN-iPSCs-imDCs on T-cell stimulatory function, and regulatory T (Treg) cell proliferative function in vitro were analyzed by mixed lymphocyte reaction. (newswise.com)
  • This study demonstrated that SN-iPSCs-imDCs have potential applications in vitro and in vivo for induction of immunotolerance following organ transplantation. (newswise.com)
  • This study focused on exploring sinomenine (SN) to promote differentiation of induced pluripotent stem cells (iPSCs) into imDCs (SN-iPSCs-imDCs), and found SN-iPSCs-imDCs with worse donor-specific T-cell stimulatory function, and higher regulatory T-cell proliferative function in vitro and in vivo to induce high immune tolerance. (newswise.com)
  • Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cell that can be generated directly from a somatic cell. (wikipedia.org)
  • citation needed] Since iPSCs can be derived directly from adult tissues, they not only bypass the need for embryos, but can be made in a patient-matched manner, which means that each individual could have their own pluripotent stem cell line. (wikipedia.org)
  • dubious - discuss] In his Nobel seminar, Yamanaka cited the earlier seminal work of Harold Weintraub on the role of myoblast determination protein 1 (MyoD) in reprogramming cell fate to a muscle lineage as an important precursor to the discovery of iPSCs. (wikipedia.org)
  • iPSCs are typically derived by introducing products of specific sets of pluripotency-associated genes, or "reprogramming factors", into a given cell type. (wikipedia.org)
  • Unlike the first generation of iPSCs, these second generation iPSCs produced viable chimeric mice and contributed to the mouse germline, thereby achieving the 'gold standard' for pluripotent stem cells. (wikipedia.org)
  • The cells are formally termed "iPSCs. (psychiatrictimes.com)
  • A few months earlier, scientists reprogrammed human induced pluripotent stem cells (iPSCs) into liver buds that also took on three dimensions. (the-scientist.com)
  • In addition to human iPSCs, it can be used to replate culture of primary neurons or to resuspend them for fact sorting or single cell sequencing. (jove.com)
  • They called the pluripotent stem cells that they produced induced pluripotent stem cells (iPSCs) because they had induced the adult cells, called differentiated cells, to become pluripotent stem cells through genetic manipulation. (asu.edu)
  • METHODS: Here, we have carried out a longitudinal analysis of the transcriptome of human iPSCs undergoing neural induction. (ox.ac.uk)
  • This dynamical analysis of transcriptional changes provides a unique perspective of the widespread remodelling of the cell machinery that occurs during neural induction of human iPSCs. (ox.ac.uk)
  • We do this by using induced pluripotent stem cells (iPSCs) to examine neuronal development and degeneration on specific genetic backgrounds (our disease focus is increasingly on ASD and Alzheimer's Disease). (ox.ac.uk)
  • Cells from the AML-infiltrated skin from an M6 patient were infected with a lentivirus carrying OCT4, SOX2, KLF4 and C-MYC to induce iPSCs. (bvsalud.org)
  • ALP staining confirmed that these cells exhibited positive staining and had the characteristics of iPSCs. (bvsalud.org)
  • The most well-known type of pluripotent stem cell is the embryonic stem cell. (wikipedia.org)
  • Patient-matched embryonic stem cell lines can now be derived using somatic cell nuclear transfer (SCNT). (wikipedia.org)
  • They hypothesized that genes important to embryonic stem cell (ESC) function might be able to induce an embryonic state in adult cells. (wikipedia.org)
  • Here we performed a systematic analysis of two different induced pluripotent stem cell lines (iPSC 3.4 and 4.1) and an embryonic stem cell (ESC) line-derived cardiac myocytes at two different developmental stages. (nih.gov)
  • A Targeted NKX2.1 Human Embryonic Stem Cell Reporter Line Enables Identification of Human Basal Forebrain Derivatives. (edu.au)
  • Embryonic stem cell lines derived from human blastocysts. (springer.com)
  • Evans MD, Kelley J. US attitudes toward human embryonic stem cell research. (springer.com)
  • If one of the infected cells showed G418 resistance, then the scientists would know that one of the twenty-four genes influenced the cell to become an embryonic stem cell-like cell. (asu.edu)
  • This time, there were twenty-two cell colonies that showed a resistance to G418, meaning that there were colonies in which the cells exhibited embryonic stem cell properties. (asu.edu)
  • Our goal is to generate a toolbox of human embryonic stem cell lines to monitor blood forming (hematopoietic) stem cell development in culture. (ca.gov)
  • To help overcome these bottlenecks, we will generate a suite of human embryonic stem cell reporter lines that can be used to monitor key milestones in blood stem cell development. (ca.gov)
  • The related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through acetyltransferase-independent mechanisms. (axonmedchem.com)
  • We explore the method on three circuits for haematopoiesis and embryonic stem cell development for commitment and reprogramming scenarios and illustrate how the method can be used to determine sequential steps for onsets of external factors, essential for efficient reprogramming. (lu.se)
  • Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
  • In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
  • Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system. (biomedcentral.com)
  • Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 + hematopoietic cell induction from mouse PSCs. (biomedcentral.com)
  • Protein-iPS cells were biologically and functionally very similar to ES cells and differentiated into 3 germ layers in vitro. (ewha.ac.kr)
  • We are working to generate red blood cells in vitro from pluripotent stem cells, however the extremely large cell numbers involved pose unique challenges to culture technology and scale-up. (selectbiosciences.com)
  • Stem cell-derived cardiac myocytes are potential sources for testing cardiocytoprotective molecules against ischemia/reperfusion injury in vitro. (nih.gov)
  • This protocol describes a detailed procedure for resuspending and culturing human stem cell derived neurons that were previously differentiated from neural progenitors in vitro for multiple weeks. (jove.com)
  • The recent development of stem cell research and the possibility of generating cells that can be stably and permanently modified in their genome open a broad horizon in the world of in vitro modeling. (wjgnet.com)
  • In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
  • Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)
  • The stem cell field witnessed a genuine breakthrough when a combination of solely four transcription factors ( Oct3 / 4 , Sox2 , Klf4 and c-Myc, OSKM ) proved enough to revert, in vitro, the differentiated status of a variety of cell types back to pluripotency, giving rise to so-called induced pluripotent stem (iPS) cells. (springer.com)
  • These lines will serve as tools to identify factor combinations to improve the in vitro differentiation of hESCs to functional blood stem cells. (ca.gov)
  • Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)
  • The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)
  • The present study aimed to establish an induced pluripotent stem cell (iPSC) line from acute myelogenous leukemia (AML) cells in vitro and identify their biological characteristics. (bvsalud.org)
  • Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)
  • This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
  • Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)
  • The process is so inefficient that scientists typically only get 0.01 percent of a sample of human skin, or fibroblast, cells to form iPS cell colonies after they infect fibroblasts with the retroviruses used to induce pluripotency. (thestemcellfoundation.com)
  • After 14 days, when cells start to become fully pluripotent, 10 to 20 percent of the mouse cells that were grown with vitamin C expressed genes associated with pluripotency compared with only 0.1 to 0.2 percent of the colonies grown without vitamin C. The group saw a similar improvement in human fibroblast reprogramming, from 0.01 percent in the absence of vitamin C to 1 percent in the presence. (thestemcellfoundation.com)
  • Pei believes that ascorbic acid is spurring the induction of pluripotency in mouse and human cells through both its anti-oxidant properties as well as an as-yet unknown mechanism. (thestemcellfoundation.com)
  • Kim has developed a system to improve the safety of iPS cells by delivering the four proteins that induce pluripotency directly into cells. (thestemcellfoundation.com)
  • This approach could be adding the four pluripotency-inducing proteins to cells to jumpstart the reprogramming 'machine' and then improving the efficiency with a combination of chemicals, including vitamin C. Pei is currently trying to optimize the medium in which cells are grown. (thestemcellfoundation.com)
  • Transcription factors have an important role in the ability of a cell to self-renew and also differentiate into most cell types, also known as pluripotency 1 . (biolegend.com)
  • INTRODUCTION: Neural induction of human induced pluripotent stem cells represents a critical switch in cell state during which pluripotency is lost and commitment to a neural lineage is initiated. (ox.ac.uk)
  • RESULTS: In addition to modules that govern loss of pluripotency and gain of neural ectoderm identity, we discover other modules governing cell cycle and metabolism. (ox.ac.uk)
  • Further CRISPRi inhibition of OTX2 prior to neural induction promotes an accelerated loss of pluripotency and a precocious and aberrant neural induction disrupting some of the previously identified modules. (ox.ac.uk)
  • DISCUSSION: We infer that OTX2 has a diverse role during neural induction and regulates many of the biological processes that are required for loss of pluripotency and gain of neural identity. (ox.ac.uk)
  • Complex haploinsufficiency in pluripotent cells yields somatic cells with DNA methylation abnormalities and pluripotency induction defects. (cdc.gov)
  • A process where fully differentiated or specialized cells revert to pluripotency or a less differentiated cell type. (bvsalud.org)
  • Because they can propagate indefinitely, as well as give rise to every other cell type in the body (such as neurons, heart, pancreatic, and liver cells), they represent a single source of cells that could be used to replace those lost to damage or disease. (wikipedia.org)
  • Moreover, tranylcypromine treatment affects neurons and astrocytes, which impairs cell density and arrangement. (frontiersin.org)
  • It involves answering some simple questions: What if you took the skin cells from patients who had schizophrenia and turned them into neurons? (psychiatrictimes.com)
  • This protocol facilitates the use of human pluripotent stem cell-derived neurons for high content screening applications. (jove.com)
  • Human pluripotent stem cell-derived neurons are increasingly relevant in the areas of basic research, drug development, and degenerative medicine. (jove.com)
  • Rapid Single-Step Induction of Functional Neurons from Human Pluripotent Stem Cells', Zhang et al. (addgene.org)
  • Repressor element 1-silencing transcription factor deficiency yields profound hearing loss through Kv7.4 channel upsurge in auditory neurons and hair cells. (ox.ac.uk)
  • Stem Derived Dopamine Neurons: Will They Replace DBS as the Leading Neurosurgical Treatment for Parkinson's Disease? (lu.se)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
  • Human pluripotent stem cells (hPSCs) hold great promise for applications in cell therapy and drug screening in the cardiovascular field. (mbexc.de)
  • Using our recently established haploid human pluripotent stem cells (hPSCs), we generated a genome-wide loss-of-function library targeting 18,166 protein-coding genes to define the essential genes in hPSCs. (nature.com)
  • Dr. Wing Chang, Scientist at STEMCELL Technologies, gives a tutorial focusing on fibroblast reprogramming and culture of naive-like human pluripotent stem cells (hPSCs). (stemcell.com)
  • Recent advances have shown that culture conditions can shift human pluripotent stem cells (hPSCs) between different pluripotent states: from ground or naĂŻve-like to primed states. (stemcell.com)
  • Recent breakthroughs in human pluripotent stem cells (hPSCs)-derived cerebral organoids offer a promising approach for investigating the mystery of human brain ( 19 ). (frontiersin.org)
  • Little has been studied regarding EVs derived from human pluripotent stem cells (hPSC-EVs), even though hPSCs offer good opportunities for induction of tissue regeneration and unlimited proliferative ability. (wjgnet.com)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • A high level of ROS is definitely very bad for the fibroblasts,' Pei says, because it induces cell death at a faster rate. (thestemcellfoundation.com)
  • The researchers started with fibroblast cells from mouse embryos, which have been shown to revert to iPS cells at a higher frequency than human fibroblasts. (thestemcellfoundation.com)
  • The fibroblasts were engineered so that any cells reactivating the ESC-specific gene, Fbx15, could be isolated using antibiotic selection. (wikipedia.org)
  • We report that that a single transfer of embryonic stem (ES) cell-derived proteins into primarily cultured adult mouse fibroblasts, rather than repeated transfer or prolonged exposure to materials, can achieve full reprogramming up to the pluripotent state without the forced expression of ectopic transgenes. (ewha.ac.kr)
  • Applying MPTR to dermal fibroblasts from middle-aged donors, we found that cells temporarily lose and then reacquire their fibroblast identity, possibly as a result of epigenetic memory at enhancers and/or persistent expression of some fibroblast genes. (elifesciences.org)
  • Instead, stem cells are being induced to make fibroblasts by implanting fibroblast DNA into a ghost cell. (gcimagazine.com)
  • Even the use of stem cells in plastic surgery is primarily with the hope that they will become growth factor production cells, such as fibroblasts or macrophages. (gcimagazine.com)
  • It is this special shape that makes growth factors from stem cells and fibroblasts much more stable and active in the skin. (gcimagazine.com)
  • Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
  • Both in animal models and humans, it has been shown that cell therapy can induce therapeutic angiogenesis, making mesenchymal stromal cell-based therapy one of the most promising therapeutic alternatives. (unav.edu)
  • The aim of this study is to evaluate the feasibility, safety, and efficacy of cell therapy based on mesenchymal stromal cells derived from adipose tissue intramuscular administration to patients with type 2 diabetes mellitus with critical limb ischemia and without possibility of revascularization. (unav.edu)
  • Mesenchymal stromal cells (MSCs) have shown benefit in other inflammatory diseases. (unav.edu)
  • Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)
  • Although hematopoietic stem and progenitor cells (HSPCs) enable autologous treatment of blood disorders, de novo generation of hematopoietic cells from pluripotent stem cells still compromises the unlimited high yield and rich hematopoiesis engraftment potential [ 2 ]. (biomedcentral.com)
  • STEMdiff™ Neural Progenitor Medium is a defined and serum-free medium for the expansion of neural progenitor cells (NPCs) derived from human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells using STEMdiff™ Neural Induction Medium (Catalog #05835). (stemcell.com)
  • In November, American and Spanish researchers published their data on functional, renal progenitor-like cells developed from human stem cells. (the-scientist.com)
  • Use of progenitor cell lines can eliminate the need to culture challenging human ES cells. (ddw-online.com)
  • Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
  • However, progenitor cells can only differentiate into a limited number of cell types. (ddw-online.com)
  • Progenitor cells can be far easier to handle in culture than ES cells. (ddw-online.com)
  • For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)
  • Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)
  • This process relies on the formation of a blastema, a pool of progenitor cells that is formed in response to amputation. (lu.se)
  • We know that innate immune cells are required for blastema progenitor cell formation, but are lacking an understanding of the immune cell processes and functions that are required for inducing and maintaining these progenitors cells. (lu.se)
  • The path that Pei's group took to identifying the beneficial role of vitamin C started with the realization that the factors that induce cells to become pluripotent were causing the cells to make the free radicals known as reactive oxygen species (ROS). (thestemcellfoundation.com)
  • In 2006, Takahashi and Yamanaka selected twenty-four candidate genes as factors that they hypothesized could possibly induce somatic cells to become pluripotent, and they began to test them one at a time. (asu.edu)
  • Once individual lines have been validated, lines that contain multiple fluorescent reporters will be generated, and a multi factor screen will be performed to optimize conditions that induce these blood stem cell regulators. (ca.gov)
  • Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. (novusbio.com)
  • Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. (novusbio.com)
  • Aging cell 2023 8 e13964. (cdc.gov)
  • However, since the generation of embryonic stem cells involves destruction (or at least manipulation) of the pre-implantation stage embryo, there has been much controversy surrounding their use. (wikipedia.org)
  • Efficient generation of embryonic stem cells from single blastomeres of cryopreserved mouse embryos in the presence of signalling modulators. (axonmedchem.com)
  • The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
  • We interestingly found that our 3D+OP9 system enriched a higher percentage of CD201 + c-kit + cells that showed more similar HSC-like features such as transcriptome level and CFU formation ability than CD201 - c-kit + cells, which have not been reported in the field of mouse PSCs hematopoietic differentiation. (biomedcentral.com)
  • Moreover, CD201 + hematopoietic cells remained in a relatively slow cycling state, consistent with high expression levels of P57 and Ccng2. (biomedcentral.com)
  • Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
  • In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)
  • RUNX1-deficient mice fail to generate hematopoietic stem cells. (biolegend.com)
  • Currently, we lack markers that we can use to pinpoint the developmental stage of human ES cell derived candidate hematopoietic stem cells (HSC), and whether they have established the correct molecular machinery required for proper function. (ca.gov)
  • The major shortcoming in generating functional hematopoietic stem cells (HSC) from pluripotent stem cells is the lack of adequate markers for specification and maintenance of functional human HSCs. (ca.gov)
  • Recent breakthroughs in human pluripotent stem cell-derived cerebral organoids provide a valuable platform for investigating the human brain after different drugs treatments and for understanding the complex genetic background to human pathology. (frontiersin.org)
  • Here, we identified tranylcypromine, which is used to treat refractory depression, caused human-induced pluripotent stem cell-derived brain organoids neurotoxicity, leading to decreased proliferation activity and apoptosis induction. (frontiersin.org)
  • Sozzi E, Nilsson F, Kajtez J, Parmar M, Fiorenzano A. Generation of Human Ventral Midbrain Organoids Derived from Pluripotent Stem Cells . (lu.se)
  • These unlimited supplies of autologous cells could be used to generate transplants without the risk of immune rejection. (wikipedia.org)
  • This presentation will describe the current status of strategies to generate induced pluripotent stem (iPS) cells from mouse and human somatic cells for patient-specific disease modeling and cell therapeutic applications. (selectbiosciences.com)
  • The study is an interdisciplinary effort to generate safe and effective chimeric antigen receptor (CAR)-T cell therapy for cancer that will be done by doing a deep-dive into understanding the relationship between the metabolism and anti-tumor functions of CAR-T cells. (uclahealth.org)
  • Researchers were able to generate in vivo more primitive forms of stem cells that had been accomplished before. (the-scientist.com)
  • To track the location and quantity of transplanted cells in recipient small animal model, we will generate hESC lines with in vivo reporter system that combines bioluminescent or PET imaging, and serum-based assay. (ca.gov)
  • Our results provide an alternative and safe strategy for the reprogramming of somatic cells that can be used to facilitate pluripotent stem cell-based cell therapy. (ewha.ac.kr)
  • Our in vivo tracking tools will be broadly relevant and not restricted to studying the in vivo biology of blood forming cells. (ca.gov)
  • Improved understanding of the pathology of histiocytic disorders requires knowledge of the origins, biology, and physiology of the cells involved. (medscape.com)
  • To enhance the survival of cells and their likelihood of becoming iPS cells, Pei's team tested a variety of chemicals with anti-oxidant properties. (thestemcellfoundation.com)
  • One possible mechanism to explain this improved survival is that the cells exposed to ascorbic acid had lower levels of a tumor suppressor protein called p53, which can elicit cell death. (thestemcellfoundation.com)
  • It is involved in p53 pathways and is implicated in cell death/survival signaling, the cell cycle, and differentiation, thereby playing a regulatory role in carcinogenesis [ 12 ]. (hindawi.com)
  • Rb homeostasis is also essential for self-renewal and survival of human embryonic stem cells 10 . (biolegend.com)
  • It was suggested that lactate promoted the survival of iPS cells cultured in a medium without glucose and supplemented with galactose. (spandidos-publications.com)
  • Glucose is essential for cell survival and is metabolized to pyruvate ( 13 ). (spandidos-publications.com)
  • Background: Bivalent chromatin domains consisting of the activating histone 3 lysine 4 trimethylation (H3K4me3) and repressive histone 3 lysine 27 trimethylation (H3K27me3) histone modifications are enriched at developmental genes that are repressed in embryonic stem cells but active during differentiation. (researchgate.net)
  • Central to this is the transient increase in H3K4-trimethylation at developmental genes during G1, thereby creating a 'window of opportunity' for cell-fate specification. (researchgate.net)
  • The developmental capacity of nuclei taken from differentiating endoderm cells of Xenopus laevis. (springer.com)
  • The developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles. (springer.com)
  • The developmental capacity of nuclei transplanted from keratinized skin cells of adult frogs. (springer.com)
  • Based on observations that chalcones can stimulate BMP4 signaling pathways, we hypothesized their utility in cardiac mesoderm induction. (mbexc.de)
  • Cysteines are an amino acid which are involved in pathways that feed cancer cells and provide building blocks for cancer cell growth. (uclahealth.org)
  • Dendritic cells appear to develop in several pathways. (medscape.com)
  • An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
  • Accordingly, in various biological contexts, EVs are reported to engage in immune modulation, senescence, and cell proliferation and differentiation. (wjgnet.com)
  • Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
  • We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
  • BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)
  • Each category of histiocytosis can be traced to reactive or neoplastic proliferation in one of these cell lineages. (medscape.com)
  • The iPSC technology was pioneered by Shinya Yamanaka and Kazutoshi Takahashi in Kyoto, Japan, who together showed in 2006 that the introduction of four specific genes (named Myc, Oct3/4, Sox2 and Klf4), collectively known as Yamanaka factors, encoding transcription factors could convert somatic cells into pluripotent stem cells. (wikipedia.org)
  • It is also clear that pro-mitotic factors such as C-MYC/L-MYC or repression of cell cycle checkpoints, such as p53, are conduits to creating a compliant cellular state for iPSC reprogramming. (wikipedia.org)
  • Our study provided evidence that an iPSC line derived from AML cells was successfully established. (bvsalud.org)
  • hPSC-enriched essential genes mainly encode transcription factors and proteins related to cell-cycle and DNA-repair, revealing that a quarter of the nuclear factors are essential for normal growth. (nature.com)
  • Fig. 1: Identifying transcription factors that reprogram starting cells to target cell types. (nature.com)
  • Fig. 3: Use of histone mark and EP300 annotation does not significantly affect transcription factor recovery in liver cells. (nature.com)
  • The capability of these cells to differentiate depends on the stem cell type, the regulation of gene expression by various transcription factors and interaction with the stem cell niche 1,4 . (biolegend.com)
  • The underphosphorylated, active form of Rb interacts directly with E2F1 , leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. (biolegend.com)
  • RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. (biolegend.com)
  • After 7 days of culture, the cells were subjected to reverse transcription‑quantitative PCR analysis. (spandidos-publications.com)
  • This subset of transcription factors, known as pioneer transcription factors (PTFs), possess the ability to engage closed chromatin that is not accessible by other types of transcription factors, establish gene expression, and elicit cell reprogramming. (cytoskeleton.com)
  • The transcription factors Oct4, Sox2, and Klf4 were found to trigger endogenous expression of pluripotent genes [2] and were identified as PTFs due to their ability to access closed chromatin [3] . (cytoskeleton.com)
  • Although many of the key transcription factors involved in neural induction are known, we know little of the temporal and causal relationships that are required for this state transition. (ox.ac.uk)
  • Using the temporal relationships between the changing profile of key transcription factors and subsequent changes in their target gene expression profiles, we have identified distinct functional modules operative throughout neural induction. (ox.ac.uk)
  • We then focussed on OTX2, one of the most precociously activated transcription factors during neural induction. (ox.ac.uk)
  • The ability to reprogram skin cells to pluripotent stem cells by a simple gene-expression formula opened up a world of new experiments. (the-scientist.com)
  • Yamanaka received the Nobel Prize in Physiology or Medicine in 2012, along with John Gurdon, as their work showed scientists how to reprogram mature cells to become pluripotent. (asu.edu)
  • The Dolly experiment showed that scientists could reprogram the nucleus of somatic cells by transferring the contents of the nucleus into oocytes that have had their nuclei removed, a technique called somatic cell nuclear transfer (SCNT). (asu.edu)
  • This presentation describes the current state of the stem cells marketplace as it relates to their utilization for pharmaceutical screening as well as their development for cellular therapy. (selectbiosciences.com)
  • Details are provided about the quantitative market opportunity for various stem cell classes as well as the current state of the clinical trials space wherein stem cell-based therapeutics are being evaluated for therapy in various disease classes. (selectbiosciences.com)
  • Therefore, EVs could be key elements for potential off-the-shelf cell-free therapy. (wjgnet.com)
  • JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)
  • Also, the multidisciplinary coordination exercise needed to develop this clinical trial protocol will undoubtfully be useful to conduct academic clinical trials in the field of cell therapy in the near future. (unav.edu)
  • Our goal is to understand the structural and functional properties of excitable cells in the heart and brain across multiple length scales in order to decipher disease-relevant, nanoscale functional units in heart and nerve cells. (mbexc.de)
  • Novel culture and cryopreservation systems for human pluripotent stem cells and neural stem cells will be presented and their advantages and applicability in the production of high quality advanced therapeutic products or functional screening tools for preclinical research discussed. (selectbiosciences.com)
  • Using these reporter lines to monitor the induction of HSC "stemless' program, we are making progress in optimizing differentiation conditions toward generating functional HSCs for transplantation. (ca.gov)
  • Strikingly, some of these functional modules are retained throughout neural induction, even though the gene membership of the module changes. (ox.ac.uk)
  • The primitive streak gene Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells. (edu.au)
  • As ChIP-seq cannot establish physical co-occurrence of two marks on the same allele, admixture of cells that either express (green) or do not express (red) the gene in focus could explain the occurrence of both marks as well as the low expression level in the overall population. (researchgate.net)
  • In contrast, in the case of ''true'' bivalency, virtually all cells in the population carry both marks simultaneously at the promoter in question, leading to low, if any, expression for that gene in all cells. (researchgate.net)
  • Histone modifications and chromatin-associated protein complexes are crucially involved in the control of gene expression, supervising cell fate decisions and differentiation. (researchgate.net)
  • Each gene was inserted near the mouse Fbx15 gene, a gene that embryonic stem cells express during development in mice. (asu.edu)
  • In eukaryotic organisms, there is a multitude of specialized, differentiated cells that are formed by specific gene programs. (cytoskeleton.com)
  • In 2006, Kazutoshi Takahashi and Shinya Yamanaka reprogrammed mice fibroblast cells, which can produce only other fibroblast cells, to become pluripotent stem cells, which have the capacity to produce many different types of cells. (asu.edu)
  • The researchers added all of the twenty-four retroviral factors at the same time into mouse fibroblast cells. (asu.edu)
  • F urther complicating matters, human ES cells are typically co-cultured with feeder layers of mouse fibroblast cells. (ddw-online.com)
  • The ultimate goal is to make iPS cells a valid therapeutic option by replacing the potentially dangerous retroviruses with a safer cocktail of proteins and chemicals, including vitamin C. (thestemcellfoundation.com)
  • The opportunities and challenges of development of stem cell-based therapeutic interventions for joint surface repair for the treatment and prevention of osteoarthritis will be discussed. (selectbiosciences.com)
  • Our goal is to develop tools that address major bottlenecks that have prevented the generation of blood forming stem cells in culture for therapeutic use. (ca.gov)
  • The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • Normalized area under rank recall curve values for all methods are available in Supplementary Tables 2 - 6 and for epigenomic marks of liver cells in Extended Source Data Fig. 3 . (nature.com)
  • The technology involves capturing vesicles released from liver cancer cells from patient plasma, and measuring molecules inside the vesicles that can inform us about the stage of the cancer. (uclahealth.org)
  • Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. (springer.com)
  • Hepatocytes derived from iPS cells are suitable for transplantation into patients with liver insufficiency and for use in toxicity tests ( 2 ). (spandidos-publications.com)
  • Therefore, on the basis of the data available at this time, it was not possible to identify a mechanism for the induction of myeloid leukemia in humans. (who.int)
  • By adding vitamin C to the medium in which human skin cells are grown, the researchers managed to turn 1 to 2 percent, compared to 0.01 percent, of cells into iPS cell colonies. (thestemcellfoundation.com)
  • Upon introduction of reprogramming factors, cells begin to form colonies that resemble pluripotent stem cells, which can be isolated based on their morphology, conditions that select for their growth, or through expression of surface markers or reporter genes. (wikipedia.org)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • Our scientists have developed a wide array of stem cell-focused reagents and resources for many applications including flow cytometry , western blotting , ELISAs , and recombinant proteins for cell differentiation. (biolegend.com)
  • Use of feeder layers requires two cell types to be maintained in parallel and introduces mouse proteins into the culture system. (ddw-online.com)
  • [ 15 ] The surface of the APC contains 2 peptide-binding proteins (ie, major histocompatability complex [MHC] classes I and II), which can stimulate cytotoxic T (T C ) cells, regulatory T (Treg) cells, and helper T (T H ) cells. (medscape.com)
  • Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
  • However, these cells have limitations, such as rapid maturation and a short lifespan in vivo . (newswise.com)
  • The c-kit + cells from 3D self-assembling peptide induction system followed by the OP9 coculture system possessed apparently superiority in terms of in vivo repopulating activity than that of 3D induction system followed by the 0.1% gelatin culture. (biomedcentral.com)
  • Furthermore, protein-iPS cells possessed in vivo differentiation (well-differentiated teratoma formation) and development (chimeric mice generation and a tetraploid blastocyst complementation) potentials. (ewha.ac.kr)
  • Such a description excludes diseases in which infiltration of these cells occurs in response to a primary pathology. (medscape.com)
  • In the April issue of Psychiatric Times , I discussed how adult stem cells were being used to aid in research on a neurological disorder (spinal muscular atrophy). (psychiatrictimes.com)
  • Stem cells are unique due to their ability to limitlessly self-renew and differentiate into each cell type in the adult body. (biolegend.com)
  • Viable offspring derived from fetal and adult mammalian cells. (springer.com)
  • Takahashi and Yamanaka's 2006 and 2007 experiments showed that scientists can prompt adult body cells to dedifferentiate, or lose specialized characteristics, and behave similarly to embryonic stem cells (ESCs). (asu.edu)
  • Efficient Endoderm Induction from Human Pluripotent Stem Cells by Logically Directing Signals Controlling Lineage Bifurcations. (edu.au)
  • Fourier transform infrared microspectroscopy identifies early lineage commitment in differentiating human embryonic stem cells. (edu.au)
  • Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
  • Identifying the right cocktail of media conditions, supplements and growth factors that successfully drive stem cells toward a desired lineage on a reproducible basis is a time-consuming, iterative exercise. (ddw-online.com)
  • Systems analysis identifies other modules associated with cell fate commitment, genome integrity, stress response and lineage specification. (ox.ac.uk)
  • or movements in a free energy landscape such that lineage choices are paths between stable cell states. (lu.se)
  • However, during the generation process of induced pluripotent stem (iPS) cells, genetic manipulation of certain factors may cause tumorigenicity, which limits further application. (ewha.ac.kr)
  • However, a general method for the identification of reprogramming factors to create an arbitrary cell type is an open problem. (nature.com)
  • The Construction and Identification of Induced Pluripotent Stem Cells Derived from Acute Myelogenous Leukemia Cells. (bvsalud.org)
  • The results from a study that used cells derived from a deceased patient's skin tissue has recently been published. (psychiatrictimes.com)
  • 2 It was reported that stem cells have an ability to transform themselves into different cell types, depending on the tissue, essentially at the scientist's whim. (psychiatrictimes.com)