Induction of heme oxygenase 1 heme oxygenase 1. Medical search. Frequent questions

Heme oxygenase 1 (HMOX1, commonly HO-1) is a member of the heat shock protein (HSP) family identified as HSP32. (wikipedia.org)
HO-1 is a 32kDa enzyme which contains 288 amino acid residues encoded by the HMOX1 gene. (wikipedia.org)
The discovery of the antioxidant response element (ARE) have led to the conclusion that the battery of genes, including glutamate-cysteine ligase (GCL), thioredoxin reductase 1 (Txnrd1), NAD(P)H-quinone oxidoreductase 1 (NQO1) and heme oxygenase-1 (HMOX1) is regulated through Nrf2 binding to this consensus binding sequence [ 3 ]. (springer.com)
HMOX1 and HMOX2 belong to the heme oxygenase family. (genetex.com)
Heme oxygenase-1 (Hmox1) is a stress-inducible protein crucial in heme catabolism. (springer.com)
This was accompanied by higher numbers of Ly6C hi monocytes in peripheral blood, as well as higher expression of monocyte chemoattractant protein-1 and adhesion molecules in the hearts of MI-operated Hmox1 −/− mice. (springer.com)
Heme oxygenase-1 (Hmox1) catalyzes the conversion of heme to biliverdin, carbon monoxide (CO), and ferrous iron (Fe 2+ ). (springer.com)

Heme oxygenase, or haem oxygenase, (HMOX, commonly abbreviated as HO) is an enzyme that catalyzes the degradation of heme to produce biliverdin, ferrous ion, and carbon monoxide. (wikipedia.org)
Heme oxygenase (alternatively spelled using haem or oxidase) catalyzes the degradation of heme to biliverdin/bilirubin, ferrous ion, and carbon monoxide. (wikipedia.org)
The major characteristics of Nrf2 are to some extent mimicked by Nrf2-dependent genes and their proteins including heme oxygenase-1 (HO-1), which besides removing toxic heme, produces biliverdin, iron ions and carbon monoxide. (springer.com)
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. (genetex.com)
The Special Issue will continue to focus on the most up-to-date knowledge concerning the pharmacology and clinical applications of the enzyme heme oxygenase-1 (HO-1) that plays a critical role in the catabolism of heme to the bile pigment bilirubin, generating carbon monoxide (CO) and releasing iron, which is sequestered by ferritin. (mdpi.com)

Heme proteins such as myoglobin or hemoglobin, when released into the extracellular space, can instigate tissue toxicity. (jci.org)
In the glycerol model of this syndrome, we demonstrate that the kidney responds to such inordinate amounts of heme proteins by inducing the heme-degradative enzyme, heme oxygenase, as well as increasing the synthesis of ferritin, the major cellular repository for iron. (jci.org)
The transcription factor NF-E2-related factor 2 (Nrf2) plays a critical role in the mammalian response to chemical and oxidative stress through induction of phase II detoxification enzymes and oxidative stress response proteins. (cdc.gov)
In its inactive condition, Nrf2 is normally sequestered in the cytoplasm and from the actin anchored Kelch-like ECH-associated proteins 1 (Keap1)7,8. (mingsheng88.org)
In the nucleus, Nrf2 identifies and binds to a conserved antioxidant response component (ARE) and induces transcription of the battery pack of chemoprotective antoxidant genes9,10, including those encoding antioxidant proteins like heme oxygenase (HO-1)11. (mingsheng88.org)
This Special Issue will highlight the wealth of research on the hereditary and acquired disorders of heme metabolism and the role of dietary regulation of heme-containing proteins via hormonal and chemical actions as well as on the genetic and acquired disorders associated with such diseases as neonatal jaundice and hemochromatosis. (mdpi.com)
HCV proteins increase expression of heme oxygenase-1 (HO-1) and decrease expression of Bach1 in human hepatoma cells. (wakehealth.edu)

Oral lactoferrin prevents body weight loss and increases cytokine responses during herpes simplex virus type 1 infection of mice. (microbiologyresearch.org)
Furthermore, an increased protein expression of heme oxygenase-1 in the liver tissues of the I/R mice was attenuated by the administration of GP. (spandidos-publications.com)
Much higher HO-1 protein levels were found in tissues of rats rather than mice. (elsevierpure.com)
The ELISA assay for HO-1 described here will be useful for HO-1 research studies in tissues and cell cultures of rats and mice. (elsevierpure.com)
The number of HO-1 positive alveolar macrophages was significantly elevated in lungs of mice treated with OVA + PM/OVA compared to OVA/OVA. (cdc.gov)

Upon loss of attachment, ATF4 activated a coordinated program of cytoprotective autophagy and antioxidant responses, including induced expression of the major antioxidant enzyme heme oxygenase 1 (HO-1). (jci.org)
We provide the first in vivo evidence that induction of heme oxygenase coupled to ferritin synthesis is a rapid, protective antioxidant response. (jci.org)
encoded by Nfe2l2 gene) is a transcription factor responsible for the regulation of cellular redox balance and protective antioxidant and phase II detoxification responses in mammals [ 1 , 2 ]. (springer.com)
Soluble fractalkine (sFKN), which is secreted from damaged neurons by glutamate, promotes microglial phagocytosis of neuronal debris, and induces the antioxidant enzyme heme oxygenase-1 in microglia. (neurology-jp.org)

Protection of acute myeloid leukaemia cells from apoptosis induced by front-line chemotherapeutics is mediated by haem oxygenase-1. (nih.gov)
In addition, genetic upregulation of bilirubin production-reflecting upregulation of haem oxygenase activity or increased haem synthesis-also contributes to the elevation of unconjugated bilirubin observed in subjects with GS. (bmj.com)
These findings suggest that elevated unconjugated bilirubin-and/or possibly genetic upregulation of haem oxygenase activity-somehow prevents gain of body fat during ageing. (bmj.com)
Effects of silymarin on hepatitis C virus and haem oxygenase-1 gene expression in human hepatoma cells. (wakehealth.edu)

Pharmaceutical companies are focusing massive resources to develop a drug that targets mitochondrial dysfunction , 1 one of the major causes of the chronic diseases of aging. (lifeextension.com)
The use of 2.5% NP40 detergent greatly assists in freeing HO-1 protein from membranes and/or other cellular entities and increased the amount of HO-1 protein found in rat liver whole homogenates as well as the nuclear, mitochondrial and microsomal fractions. (elsevierpure.com)
multiple systems, including alteration of calcium mineral homeostasis4, mitochondrial perturbation, induction of NADPH oxidase appearance5, and activation of endoplasmic reticulum oxidoreductases6. (mingsheng88.org)
Lon peptidase 1 (LONP1)-dependent breakdown of mitochondrial 5-aminolevulinic acid synthase protein by heme in human liver cells. (wakehealth.edu)

This lung damage induced the cytoprotective enzyme heme oxygenase-1, which is an enzyme that catalyzes heme degradation into CO, biliverdin, and Fe2+. (umsystem.edu)

Heme oxygenase 2 (HMOX2 or HO-2) is a constitutive isoform that is expressed under homeostatic conditions in the testes, gastrointestinal tract, endothelial cells and the brain. (wikipedia.org)

the classic example is the healing process of a contusion, which forms different chromogens as it gradually heals: (red) heme to (green) biliverdin to (yellow) bilirubin which is widely known for jaundice. (wikipedia.org)
1-3 Diagnosis of GS generally requires a plasma bilirubin of 20 µM or above-alternatively, 1.2 mg/dL-but this diagnosis is not entirely objective, as non-genetic factors such as fasting status, gastrointestinal motility, enterohepatic bilirubin reabsorption/secretion and degree of light exposure can cause bilirubin levels to vary considerably over time. (bmj.com)

Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. (genetex.com)

The normal process of fracture repair begins with an immediate inflammatory response as the innate immune system (macrophages, monocytes, neutrophils, and NK cells) responds with a variety of cytokines that recruit and activate several cell types, including osteoprogenitor mesenchymal stem cells (MSCs), to the site of injury [ 1 , 2 ]. (hindawi.com)
LPS results in the overexpression and release of proinflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6), which contribute to LPS-induced multiple organs failure including myocardial depression [ 6 , 7 ]. (biomedcentral.com)
6). Lung tissue, bronchoalveolar lavage fluid (BALF) and plasma were analyzed for cellular inflammation, cytokines, immunoglobulin E and heme oxygenase-1 (HO-1) expression. (cdc.gov)
Microglia can exert neuroprotective effect by deprivation of dead cells and induction of neurotrophic factors, anti-inflammatory cytokines, and anti-oxidant enzyme in MS. Neurons are thought to be not merely passive targets of microglia but rather control microglial activity through various signals including cytokines and chemokines. (neurology-jp.org)

HO-1 upregulation was the result of simultaneous activation of ATF4 and the transcription factor NRF2, which converged on the HO1 promoter. (jci.org)
This review summarizes our knowledge about Nrf2 and HO-1 across different phyla suggesting their conservative role as stress-protective and anti-aging factors. (springer.com)
Nrf2 consists of six functional Neh domains (Neh1-Neh6), from which, the amino-terminal Neh2 domain controls binding Keap1-the inhibitor protein Kelch-like ECH-associated protein 1, that is responsible for the cytosolic sequestration of Nrf2 under physiological conditions (Fig. 2 a). (springer.com)
Nrf2 stabilization and increase in its half-life even to 200 min [ 9 ] allows nuclear translocation and activation of transcription of cytoprotective genes (Fig. 1 ). (springer.com)
Furthermore, Nrf2 translocation was accompanied by the expression of heme oxygenase-1 gene, an Nrf2-regulated gene linked to free radical production. (cdc.gov)
Translocation of Nrf2 may be a response to DM-dependent induction of free radicals and DM may act as a mammalian neurotoxin by initiating oxidative stress. (cdc.gov)
Within an HCV replicating cell lifestyle model, HCV blunted Nrf2 activation and inhibited the induction of ARE-regulated genes12. (mingsheng88.org)
Role of Bach1 and Nrf2 in up-regulation of the heme oxygenase-1 gene by cobalt protoporphyrin. (wakehealth.edu)
Ziziphus spina-christi fruit extract suppresses oxidative stress and p38 MAPK expression in ulcerative colitis in rats via induction of Nrf2 and HO-1 expression. (ac.ir)

Conversely, ablating this response with a competitive inhibitor of heme oxygenase exacerbates kidney dysfunction. (jci.org)

HO-1 is a stress-induced isoform present throughout the body with highest concentrations in the spleen, liver, and kidneys, and on the cellular level is primarily located in the endoplasmic reticulum, although it has also been reported in the mitochondria, cell nucleus, and plasma membrane. (wikipedia.org)
Some heme-degrading prokaryotic enzymes produce products such as formaldehyde rather than CO. As an example, certain pathogens such as Escherichia coli O157:H7 can express the non-CO producing ChuW isoform. (wikipedia.org)
Excellent specificity for the HO-1 isoform is shown by the failure of either the human HO-2 protein or HO-2 peptide (at concentrations as high as 1000 ng/ml) to generate any signal above background. (elsevierpure.com)

NF-kappaB-inhibited acute myeloid leukemia cells are rescued from apoptosis by heme oxygenase-1 induction. (nih.gov)
HO-1 and their products exert beneficial effects through the protection against oxidative injury, regulation of apoptosis, modulation of inflammation as well as contribution to angiogenesis. (springer.com)
NF-kappaB and AP-1 transcriptional factors contribute to the development and progression of prostate malignancy by regulating the expression of genes involved in proliferation, apoptosis, angiogenesis, and metastasis. (positivehealth.com)

FLIP regulation of HO-1 and TNF signalling in human acute myeloid leukemia provides a unique secondary anti-apoptotic mechanism. (nih.gov)
These physical properties related to ferri-/ferro-heme reduction are both structurally and biologically relevant for heme release from HPX for transport and regulation of heme oxygenase expression. (duke.edu)
Regulation of expression of the human heme oxygenase-1 gene in transfected chick embryo liver cell cultures. (wakehealth.edu)
Role of Bach-1 in regulation of heme oxygenase-1 in human liver cells: insights from studies with small interfering RNAS. (wakehealth.edu)
Differential regulation of human ALAS1 mRNA and protein levels by heme and cobalt protoporphyrin. (wakehealth.edu)

The degradation of heme forms three distinct chromogens as seen in healing cycle of a bruise. (wikipedia.org)
Many pathogens are susceptible to CO toxicity, therefore expressing non-CO producing heme degradation enzymes evades self-inflicted toxicity while meeting nutritional iron needs. (wikipedia.org)
Zinc mesoporphyrin induces rapid and marked degradation of the transcription factor Bach1 and up-regulates HO-1. (wakehealth.edu)

We show that by upregulating HO-1 activity by administering a compound, cobalt protoporphyrin IX, we were able to ameliorate the hyperinflammation, lessen tissue damage, and increase survival in animals infected with Y. pestis. (umsystem.edu)

Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at endoplasmic reticulum and nucleus by immunofluorescent analysis. (genetex.com)

In recent years, the discovery of non-heme-containing inducers of HO-1 has resulted in new discoveries regarding the metabolic and cardiovascular effects of the upregulation of HO activity. (mdpi.com)

These results establish HO-1 as a mediator of ATF4-dependent anoikis resistance and tumor metastasis and suggest ATF4 and HO-1 as potential targets for therapeutic intervention in solid tumors. (jci.org)
Moreover, Accepted for publication 28 April 2011 new mechanistic insights into the induction of ACD identify potential targets for preventive and therapeutic intervention. (cdc.gov)

A controversial third heme oxygenase (HO-3) is considered to be catalytically inactive and has been speculated to work in heme sensing or detoxification. (wikipedia.org)

The let-7 microRNA enhances heme oxygenase-1 by suppressing Bach1 and attenuates oxidant injury in human hepatocytes. (wakehealth.edu)

Increased heme oxygenase-1 gene expression in liver cells and splanchnic organs from portal hypertensive rats. (wakehealth.edu)

ATF4-deficient human fibrosarcoma cells were unable to colonize the lungs in a murine model, and reconstitution of ATF4 or HO-1 expression in ATF4-deficient cells blocked anoikis and rescued tumor lung colonization. (jci.org)
HO-1 expression was higher in human primary and metastatic tumors compared with noncancerous tissue. (jci.org)
Moreover, HO-1 expression correlated with reduced overall survival of patients with lung adenocarcinoma and glioblastoma. (jci.org)
Using RNA deep sequencing, we found that raltegravir specifically boosts the expression of anti-angiogenic factors and promotes metabolic homeostasis in FHV-1-infected cells. (microbiologyresearch.org)
ICAM-1 and gp130 expression was examined by flow cytometry. (positivehealth.com)
Treatment with alpha-tocopherol succinate (VES) inhibits NF-kappaB but augments AP-1 activity, reduces expression of IL-6, IL-8, and VEGF, suppresses cell adhesion, ICAM-1 and gp130 expression in androgen-independent PC-3, DU-145, and CA-HPV-10 cells. (positivehealth.com)
The Combination of Resveratrol and Quercetin Attenuates Metabolic Syndrome in Rats by Modifying the Serum Fatty Acid Composition and by Upregulating SIRT 1 and SIRT 2 Expression in White Adipose Tissue - Evid Based Complement Alternat Med. (qualitycounts.com)
Programmed death 1 (PD-1) is an immunoreceptor of the CD28/CTLA-4 family whose expression is induced in activated T- and B-cells and in macrophages ( 1 , 2 ). (diabetesjournals.org)
Heme oxygenase-1 (HO-1) and p-NF-κB protein expression in hearts, superoxide dismutase (SOD) activity and malondialdehyde (MDA) level in serum were used to evaluate the anti-oxidative properties of the extract. (biomedcentral.com)
In addition, the extract increased SOD activity in serum, HO-1 protein expression in hearts, and decreased TNF-α, IL-6, COX-2, p-NF-κB and p-ERK1/2 protein expression. (biomedcentral.com)
Effects of phenylarsine oxide on expression of heme oxygenase-1 reporter constructs in transiently transfected cultures of chick embryo liver cells. (wakehealth.edu)
Vascular endothelial growth factor increases heme oxygenase-1 protein expression in the chick embryo chorioallantoic membrane. (wakehealth.edu)
Reciprocal effects of micro-RNA-122 on expression of heme oxygenase-1 and hepatitis C virus genes in human hepatocytes. (wakehealth.edu)
Heme status affects human hepatic messenger RNA and microRNA expression. (wakehealth.edu)
RESULTS: We identified a significant increase in the co-expression of c-Fos and CBS positive neurons in the NTS after 1 h and 3 h of restraint water-immersion stress compared to the expressions observed in the control group. (bvsalud.org)

Ischemia/reperfusion (I/R) is a predominant cause of hepatic injury, which is of clinical significance following liver surgery, hemorrhagic shock and liver transplantation ( 1 ). (spandidos-publications.com)

Hemopexin (HPX) has two principal roles: it sequesters free heme in vivo for the purpose of preventing the toxic effects of this moiety, which is largely due to heme's ability to catalyze free radical formation, and it transports heme intracellularly thus limiting its availability as an iron source for pathogens. (duke.edu)

Genetic variations in heme oxygenase-1 and chronic hepatitis. (wakehealth.edu)

AIM: To examine whether H2S affects the nuclear factor kappa-B (NF-κB) and transient receptor potential vanilloid 1 pathways and the neurokinin 1 (NK1) receptor in the NTS. (bvsalud.org)

Stem Cell Rev 2017;13:1-9. (ac.ir)

Upstream regulatory elements in chick heme oxygenase-1 promoter: a study in primary cultures of chick embryo liver cells. (wakehealth.edu)
Mapping of the chick heme oxygenase-1 proximal promoter for responsiveness to metalloporphyrins. (wakehealth.edu)

The HO-1 system plays an important role in cellular defense in the context of several diseases or physiological processes, including cardiovascular disease, diabetes, obesity, inflammation, neurodegeneration and ageing, and transplantation, among many others. (mdpi.com)
HO-1 promotes reduction of inflammation in the cartilage, which is characteristic of cartilage degeneration. (geresdengle.com)

Unlike HO-1, HO-2 is a hemoprotein containing heme regulatory motifs that contain heme independent of the heme catabolic site. (wikipedia.org)
PD-1 has two cytoplasmic tyrosine motifs: one an immunoreceptor tyrosine-based inhibition motif and the other an immunoreceptor tyrosine-based switch motif (ITSM). (diabetesjournals.org)

Consistent with this, when the acidification of endosomes is prevented by bafilomycin then heme oxygenase-1 induction by heme-HPX no longer occurs. (duke.edu)

Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. (genetex.com)
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1500. (genetex.com)

abstract = "A double antibody capture ELISA for the HO-1 protein has been developed to separately quantitate HO-1 protein. (elsevierpure.com)

2021 May 13;9(1):35. (nih.gov)
54(1), 113-116, 2021. (sinica.edu.tw)

As expected, the highest concentration of HO-1 protein per total protein in a subcellular fraction was found in the microsomes. (elsevierpure.com)

In vitro antiviral and immunomodulatory activity of arbidol and structurally related derivatives in herpes simplex virus type 1-infected human keratinocytes (HaCat). (microbiologyresearch.org)

There are many heme degrading enzymes in nature. (wikipedia.org)
In general, only aerobic heme degrading enzymes are referred to as HMOX-like enzymes whereas anaerobic enzymes are typically not affiliated with the HMOX family. (wikipedia.org)
HO-1 enzymes are degraded via ubiquitination. (wikipedia.org)

HO-1 may also serve as a chaperone protein, engage in protein-protein interactions, be secreted into the extracellular space, and participate in other cellular functions beyond its catalytic activity. (wikipedia.org)

Here, we used felid alphaherpesvirus 1 (FHV-1), a close relative of human alphaherpesvirus 1 (HHV-1) that similarly causes ocular herpes, to characterize the host-targeted effects of raltegravir on corneal epithelial cells during an alphaherpesvirus infection. (microbiologyresearch.org)
Sterile solution in PBS containing 2.5% (v/v) 2-propanol, 25mM n-octyl-β-D-glucopyranoside and 1% (wt/v) human serum albumin. (enzolifesciences.com)
Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. (genetex.com)
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm on human renal carcinoma by immunohistochemical analysis. (genetex.com)
This ELISA for HO-1 may also work with human tissues and cells. (elsevierpure.com)
Carnosic acid attenuates cartilage degeneration through induction of heme oxygenase-1 in human articular chondrocytes. (geresdengle.com)

Whereas HO-1 has innumerable inducers, only adrenal glucocorticoids are known to induce HO-2 whereas certain other molecules may increase its catalytic velocity. (wikipedia.org)
This is especially relevant to a wide variety of natural product HO-1 inducers, which have the potential for important pharmaceutical and clinical applications, all of which will be highlighted in this Special Issue. (mdpi.com)

In general, aside from sharing the functionality of catabolizing heme, all HMOX isoforms share are signature 24-residue sequence considered to be essential for the enzymatic activity. (wikipedia.org)
The activity of HO-1 is dependent upon NADPH-Cytochrome P450 Reductase. (wikipedia.org)
Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. (genetex.com)

Mechanism of induction of heme oxygenase by metalloporphyrins in primary chick embryo liver cells: evidence against a stress-mediated response. (wakehealth.edu)
Heme biosynthesis in a chicken hepatoma cell line (LMH): comparison with primary chick embryo liver cells (CELC). (wakehealth.edu)

Anatomy7

Organisms8

Diseases2

Chemicals and Drugs83

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes25

Information Science3

HMOX17

Carbon monoxide5

Proteins7

Mice5

Antioxidant4

Haem oxygenase4

Mitochondrial4

Enzyme that catalyzes1

HMOX21

Bilirubin2

Constitutive1

Cytokines4

Nrf29

Exacerbates1

Isoform3

Apoptosis3

Regulation5

Degradation3

Cobalt protoporphyrin1

Endoplasmic1

Upregulation1

Therapeutic2

Detoxification1

Enhances1

Gene1

Expression15

Hepatic1

Vivo1

Genetic1

Transient receptor potential1

20171

Promoter2

Inflammation2

Motifs2

Occurs1

Membrane2

Abstract1

20212

Subcellular1

Structurally1

Enzymes3

Cellular1

Human6

Inducers2

Activity3

Liver2