• RESULTS: Starting from the concentration of 0.31 mg/ml, Hemidesmus indicus induced cytotoxicity and altered cell-cycle progression, through a block in the G0/G1 phase. (unibo.it)
  • Finally, we demonstrate that exhausted T cells are reinvigorated towards CLL cytotoxicity by inhibition of PDCD1/CD274 interaction in vivo. (nih.gov)
  • Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)
  • In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
  • Western blotting assay was implemented to check the levels of phosphatidylinositol 3-kinase and protein kinase B. Results revealed that piperine induced dose reliant cytotoxicity in MCF-7 human breast cancer cells. (ijpsonline.com)
  • Nevertheless, ex vivo activation with cytokines can restore cytolytic activity of NK cells against GB, indicating that NK cells have potential for adoptive immunotherapy of GB if potent cytotoxicity can be maintained in vivo . (frontiersin.org)
  • NK cells contribute to cancer immune surveillance not only by their direct natural cytotoxicity which is triggered rapidly upon stimulation through germline-encoded cell surface receptors, but also by modulating T-cell mediated antitumor immune responses through maintaining the quality of dendritic cells and enhancing the presentation of tumor antigens. (frontiersin.org)
  • Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)
  • In this investigation, the role of autophagy and apoptosis in Solanum nigrum fruit extract-induced cytotoxicity in MDA-MB-468, a triple negative breast cancer cell line was studied. (ijpsonline.com)
  • During blast crisis of chronic myelogenous leukemia (CML), abnormal granulocyte macrophage progenitors (GMP) with nuclear beta-catenin acquire self-renewal potential and may function as leukemic stem cells (Jamieson et al. (ca.gov)
  • These results provide further evidence that BCR-ABL-transformed GMP with abnormal beta-catenin activity can function as leukemic stem cells. (ca.gov)
  • Induction of apoptosis, cell-cycle analysis, levels of specific membrane differentiation markers were evaluated by flow cytometry. (unibo.it)
  • The analysis of cell differentiation by nitroblue tetrazolium (NBT) reducing activity, adherence to the plastic substrate, alpha-napthyl acetate esterase activity and morphological analysis was performed through light microscopy (LM) and transmission electron microscopy (TEM). (unibo.it)
  • The decoction caused differentiation of HL-60 cells as shown by NBT reducing activity, adherence to the plastic substrate, alpha-naphtyl acetate esterase activity, and increasing expression of CD14 and CD15. (unibo.it)
  • A range of cell types of mesenchymal origin express α-smooth muscle actin (α-SMA), a protein that plays a key role in controlling cell motility and differentiation along the fibrocyte and myofibroblast lineages. (unife.it)
  • Chronic myeloid leukemia (CML) results from BCR-ABL oncogene, which blocks CML cells differentiation and protects these cells from apoptosis. (jcancer.org)
  • Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
  • We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
  • Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
  • JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)
  • BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)
  • [ 1 ] Within the 2016 World Health Organization (WHO) category of "acute myeloid leukemia and related neoplasms," the related neoplasms derive from immature cells with evidence of myeloid differentiation, or from precursors of plasmacytoid dendritic cells. (medscape.com)
  • In response to DNA damage, myeloid leukemia cells activate a poorly understood terminal differentiation process. (psu.edu)
  • We found that NFκB-mediated p21 activation controls DNA damage-induced myeloid differentiation. (psu.edu)
  • After infection, A. phagocytophilum undergoes a developmental cycle in parasitophorous vacuoles that includes reticulated and dense forms, and this infection modulates host cell growth and differentiation [ 3 ]. (biomedcentral.com)
  • Melinda is highly proficient in the use of in vitro and in vivo assays for normal and malignant hematopoietic stem and progenitor cells, determination of self-renewal, expansion and lineage specific differentiation capabilities, and the use of induced pluripotent stem cells for the investigation of hematopoietic disorders and leukemic progression and the testing of targeted therapeutics. (edu.au)
  • Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)
  • Similar findings were made in bone marrow cells from gene-targeted mice lacking Bim and/or Puma infected with FLT3-ITD and treated with inhibitor, where loss of Puma only provided transient protection from apoptosis, but loss of Bim preserved clonal survival upon FLT3-ITD inhibition. (ashpublications.org)
  • Inhibition of cell viability was assessed with focus on apoptosis transcriptionally and translationally. (hindawi.com)
  • Immune-checkpoint proteins that are highly expressed in cancer cells play a crucial role in the inhibition of immune cells Dr. Battula is interested in identification and targeting of immune checkpoint proteins in solid tumors and hematologic malignancies. (mdanderson.org)
  • Inhibition of DNA-dependent protein kinase catalytic subunit by small molecule inhibitor NU7026 sensitizes human leukemic K562 cells to benzene metabolite-induced apoptosis. (cdc.gov)
  • Solanum nigrum (SN) is a herb with anticancer effects such as destruction of tumor cell membrane, induction of apoptosis by NF-kappaB, caspase activation, nitric oxide production and inhibition of angiogenesis [ 3 ]. (ijpsonline.com)
  • Inhibition of HOX/PBX dimer formation leads to necroptosis in acute myeloid leukemia cells ONCOTARGET. (nottingham.ac.uk)
  • However, the mechanism by which adenine inhibits the proliferation of cancer cells is sketchy. (hindawi.com)
  • In this study, we investigated the effects of adenine against the proliferation of colon cancer cells. (hindawi.com)
  • Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
  • To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
  • This activation is necessary for both cell proliferation as well as glucose uptake and use. (hindawi.com)
  • Here, we show that the NFκB pathway directly activates expression of the proliferation inhibitor p21 in response to DNA damage in myeloid leukemia cells. (psu.edu)
  • Understanding the function of lectin-glycan recognition systems in cell death will facilitate the implementation of novel therapeutic strategies aimed at controlling unbalanced cell proliferation and survival in several pathologic conditions. (nature.com)
  • Malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived hematopoietic progenitor cell results in a high circulating number of blasts, replacement of normal marrow by malignant cells, and the potential for leukemic infiltration of the central nervous system (CNS) and testes. (msdmanuals.com)
  • Imatinib inhibits proliferation and induces apoptosis in cells positive for BCR/ABL . (medscape.com)
  • Chronic myelogenous leukemia (CML), also known as chronic myeloid leukemia, is a myeloproliferative disorder characterized by increased proliferation of the granulocytic cell line without the loss of their capacity to differentiate. (medscape.com)
  • Here, we have determined the growth-inhibitory and proapototic mechanisms of 2 small molecule inhibitors of FLT3, AG1295 or PKC412, in hematopoietic progenitor cells, human leukemic cell lines, and primary AML cells expressing FLT3-ITD. (ashpublications.org)
  • BC comprises of variant subtypes and those subtypes arise from basal progenitor or luminal progenitor cells undergoing different genetic mutations[ 2 ]. (ijpsonline.com)
  • Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)
  • Moreover, the CD34 + CD38 - progenitor cells expressed variable amounts of the target receptor CD33, CD133 and c-kit (CD117) [ 20 ]. (researchsquare.com)
  • The aim of this study was to investigate the cytodifferentiative, cytostatic and cytotoxic potential of a decoction of Hemidesmus indicus's roots (0.31-3 mg/mL) on a human promyelocytic leukemia cell line (HL-60). (unibo.it)
  • Studies of cellular (nucleated blood and bone marrow cells) drug concentrations in leukemia patients have shown that peak cellular idarubicin concentrations are reached a few minutes after injection. (nih.gov)
  • We aim to generate molecular profiles of CD34+ cells isolated from peripheral blood of TKI-sensitive and -resistant chronic myeloid leukemia patients in chronic as well as acute phase. (actrec.gov.in)
  • His current research is focused on understanding the role of tumor-stroma interactions in chemo-resistance of acute myeloid leukemia (AML) cells. (mdanderson.org)
  • To identify regulators of primitive chronic myeloid leukemia (CML) cells, we performed a high-content cytokine screen using primary CD34 + CD38 low chronic phase CML cells. (haematologica.org)
  • Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm caused by an acquired 9;22-chromosomal translocation in a hematopoietic stem cell (HSC) resulting in the expression of the BCR-ABL1 fusion protein. (haematologica.org)
  • Overall, our results suggest that berberine, curcumin and picroliv were able to suppress the progression of leukemia induced by FMuLv and further support their chemopreventive potential against virally induced cancers. (oldcitypublishing.com)
  • Expression of BCR-ABL in these cells reproducibly stimulated myeloid expansion in culture and generated leukemia-initiating cells specifically in the GMP compartment. (ca.gov)
  • Leukemia stem cells (LSCs) in acute myeloid leukemia (AML) played important roles in development of leukemia, chemotherapeutic drug resistance, and disease relapse and progression. (researchsquare.com)
  • Therefore, our results suggest that CD45 dim CD34 + CD38 - CD133 + cells in AML might have the potential of leukemia stem cells. (researchsquare.com)
  • Acute myeloid leukemia (AML) is generally regarded as a stem cell disease, and it originates from a hierarchy of leukemic stem cell classes that differ in self-renewal capacity [ 1 , 2 ]. (researchsquare.com)
  • In the latest study, U of M researchers evaluated how inherited genetic polymorphisms in CD33, a protein that naturally occurs in most leukemia cells , could affect clinical outcomes of patients treated with an existing chemotherapy drug, gemtuzumab ozogamicin (GO), an immuno-conjugate between anti-CD33 antibody and a cytotoxin known as calicheamicin, which binds to CD33 on leukemic cells. (medicalxpress.com)
  • As GO is internalized by leukemia cells, the cytotoxin is released, causing DNA damage and generating leukemic cell death. (medicalxpress.com)
  • Several possible mechanisms were considered for the induction of human leukemia, such as clastogenic damage to circulatory stem cells. (who.int)
  • RUNX1-positive leukemia cells in preclinical models. (lu.se)
  • Acute myeloid leukemia (AML) is a disease with diverse genetic features of the leukemic cells and with variable outcome. (cancercentrum.se)
  • DNA methylation and copy number variation profiling of T-cell lymphoblastic leukemia and lymphoma. (cancercentrum.se)
  • We analyzed the effects of the modified B-CLL cells on the number, phenotype, and cytotoxic function of autologous T cells in 7 B-CLL patients. (houstonmethodist.org)
  • Epithelial-to-mesenchymal transition (EMT) has been shown to induce stem cell phenotype by up-regulation of GD2 in cancer cells. (mdanderson.org)
  • In recent clinical trials GO has been shown to induce remission and improve survival in subset of patients with AML, however there is wide inter-patient variation in response. (medicalxpress.com)
  • Treatment typically includes combination chemotherapy to achieve remission, intrathecal and systemic chemotherapy and/or corticosteroids for CNS prophylaxis, and sometimes cerebral irradiation for intracerebral leukemic infiltration, consolidation chemotherapy with or without stem cell transplantation, and maintenance chemotherapy for up to 3 years to avoid relapse. (msdmanuals.com)
  • Most patients up to the age of approximately 75 years should be considered for intensive combination chemotherapy with the aim to induce complete remission. (cancercentrum.se)
  • These patients' white blood cell (WBC) count is usually controlled with medication (hematologic remission). (medscape.com)
  • We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)
  • Treatment of leukemic K562 cells with cytarabine upregulated IRF8 protein and mRNA, which was correlated to increased expression of ZNF224. (lu.se)
  • By using Jurkat T cells, we now show how diphenhydramine inhibits Hv1 mediated currents, inducing a drop in intracellular pH and cellular viability. (unlp.edu.ar)
  • An herbal formula inhibits STAT3 signalling and attenuates bone erosion in collagen-induced arthritis. (greenmedinfo.com)
  • Our findings suggest that adenine inhibits the growth of colon cancer cells. (hindawi.com)
  • Several inhibitors have been described, such as AG1295, CEP701, PKC412, and SU-11 248, with cytotoxic effects to cell lines and primary AML cells in vitro expressing mutant FLT3. (ashpublications.org)
  • ROS inhibitors protect leukemic cells from TFOBO-induced cell death. (bvsalud.org)
  • Although chronic lymphocytic leukaemia (CLL) is a B cell malignancy, earlier studies have indicated a role of T cells in tumour growth and disease progression. (nih.gov)
  • In addition, the fraction of exhausted T cells increases with CLL progression. (nih.gov)
  • Cell cycle analysis suggested activation of apoptosis, without arresting cell cycle progression. (nature.com)
  • Flow cytometric analysis evidenced that piperine arrested the cell cycle at growth 2/mitosis phase, hence suppressed the breast cancer progression. (ijpsonline.com)
  • Accumulating evidence shows that cancer stem cells are key drivers of tumor formation, progression, and recurrence. (hindawi.com)
  • 3 , 4 Since 2020, older or unfit patients are treated by a non-intensive approach combining the hypomethylating agent azacitidine and the B-cell lymphoma 2 (BCL2) inhibitor venetoclax given orally until progression. (dovepress.com)
  • Incubation of the cells with N-acetyl-L-cysteine and catalase resulted in significant suppression of As4O6-induced apoptotic cell death. (spandidos-publications.com)
  • Leukemic cells treated with TFOBO displayed apoptotic cell death, as indicated by nuclear condensation, DNA fragmentation, and annexin V staining. (bvsalud.org)
  • The OX40-OX40 ligand pathway is involved in the subsequent expansion of memory antigen-specific T cells. (houstonmethodist.org)
  • Anticancer activity of adenine in colon cancer cells is attributable to the activation of apoptotic signaling and in turn the AMPK/mTOR pathway. (hindawi.com)
  • Dysregulation of the NF-κB pathway in B cells from PPARγ +/- was indicated by spontaneous NF-κB activation, as well as increased IκBα phosphorylation and gel-shift activity following LPS stimulation. (jci.org)
  • It induced apoptosis, suppressed cell migration and invasion, blocked cell cycle and phosphatidylinositol 3-kinase/ protein kinase B signaling pathway. (ijpsonline.com)
  • Our results uncover a p53-independent pathway for p21 activation involved in controlling hematopoietic cell fate. (psu.edu)
  • 1 Significant advances have been made by combining novel agents with autologous peripheral blood stem cell transplantation which allows for long-term disease-free survival in the majority of transplant-eligible patients. (haematologica.org)
  • 75 years old) followed by cytarabine consolidations and/or allogeneic stem-cell transplantation (ASCT). (dovepress.com)
  • In that the eyes can be a relative sanctuary (a place to hide from systemic chemotherapy) for leukemic cells, intraocular recurrences have been reported after both total body irradiation and systemic chemotherapy. (eyecancer.com)
  • His group is interested in cancer stem cells (CSCs), which are highly metastatic and resistant to conventional chemotherapy. (mdanderson.org)
  • For examples, Rhenen et al showed that a high percentage of CD34 + CD38 - stem cells at diagnosis significantly correlated with a high minimal residual disease frequency and subsequently to relapse especially after the third course of chemotherapy in AML patients. (researchsquare.com)
  • 1- Previous treatment of CML with any other anticancer agents including chemotherapy and/or biologic agents or prior stem cell transplant, with the exception of hydroxyurea and/or anagrelide. (who.int)
  • The culture of CD40L/OX40L-expressing B-CLL cells with autologous T cells generated CD4 + /CD8 + cytotoxic T-cell lines, which secreted interferon-γ (IFN-γ) and granzyme-B/perforin in response to autologous, but not to allogeneic, B-CLL cells or to autologous T-cell blasts. (houstonmethodist.org)
  • All cases are otherwise negative for Epstein-Barr virus (EBV) (assayed using in situ hybridization for EBV-encoded ribonucleic acid [RNA] [EBER-ISH]), B-cell, T-cell, myeloid, and natural killer (NK) cell markers, as well as for cytotoxic proteins (assayed using IHC). (medscape.com)
  • Different concentrations of Solanum nigrum extract showed cytotoxic effect on MDA-MB-468 cells. (ijpsonline.com)
  • In addition, Nutlin-3 significantly up-regulated α-SMA expression in primary endothelial cells, but not in fibroblasts and mesenchymal stem cells (MSC). (unife.it)
  • FLT3-ITD expression causes malignant transformation and factor-independent growth when expressed in factor-dependent cell lines. (ashpublications.org)
  • This revealed increased expression of B-cell lymphoma 2 associated X and reduced expression of B-cell lymphoma 2, indicating apoptosis induction by piperine. (ijpsonline.com)
  • Furthermore, we show expression of MSTN by CML mesenchymal stromal cells, and that myostatin propeptide has a direct and instant effect on CML cells, independent of myostatin, by demonstrating binding of myostatin propeptide to the cell surface and increased phosphorylation of STAT5 and SMAD2/3. (haematologica.org)
  • Furthermore, similar to T cells, specific recognition and elimination of cancer cells by NK cells can be markedly enhanced through expression of chimeric antigen receptors (CARs), which provides an opportunity to generate NK-cell therapeutics of defined specificity for cancer immunotherapy. (frontiersin.org)
  • e) induce the expression of p53. (oldcitypublishing.com)
  • The expression levels of autophagy-related genes, Lc3 and Beclin1 increased in cells treated with 1.5 mg/ml of Solanum nigrum extract. (ijpsonline.com)
  • The expression of Sp110 was silenced in HL-60 cells by RNAi using pre-designed siRNAs using the Nucleofector 96-well shuttle system (Amaxa Biosystems, Gaithersburg, MD, USA). (biomedcentral.com)
  • While Sp110 mRNA levels increased concurrently with A. phagocytophilum infections in HL-60 cells, the silencing of Sp110 expression by RNA interference resulted in decreased infection levels. (biomedcentral.com)
  • These results demonstrated that Sp110 expression is required for A. phagocytophilum infection and multiplication in HL-60 cells, and suggest a previously undescribed mechanism by which A. phagocytophilum modulates Sp110 mRNA levels to facilitate establishment of infection of human HL-60 cells. (biomedcentral.com)
  • Sp110 expression is induced in human peripheral blood leukocytes and spleen but not in other tissues [ 8 ]. (biomedcentral.com)
  • Knock down of ZNF224 with shRNA suppressed both basal and cytarabine-induced IRF8 expression. (lu.se)
  • This study aimed to investigate the effects of side-chain oxysterols on IL-1ß expression using HMC3 microglial cells and ApoE-deficient mice. (bvsalud.org)
  • Treatment of HMC3 cells with 25-hydroxycholesterol (25OHChol) and 27-hydroxycholesterol (27OHChol) led to increased IL-1ß expression at the transcript and protein levels. (bvsalud.org)
  • The extent of IL-1ß increase induced by 25OHChol and 27OHChol was comparable to that caused by oligomeric ß-amyloid, and the IL-1ß expression induced by the oxysterols was not impaired by polymyxin B, which inhibited lipopolysaccharide-induced IL-1ß expression. (bvsalud.org)
  • The pharmacological agents chlorpromazine and cyclosporin A also impaired the oxysterol-induced expression of IL-1ß and upregulation of MHC II. (bvsalud.org)
  • The expression of CD14 in monocytic cells is elevated in atherosclerotic lesions where 7-oxyterols are abundant. (bvsalud.org)
  • Our data revealed that CD14 expression was most strongly induced after treatment with 7αOHChol. (bvsalud.org)
  • These results indicate that 7-oxysterols differentially induce CD14 expression in vascular cells and contribute to the monocytic cell expression of CD14 via overlapping, but distinct, mechanisms. (bvsalud.org)
  • Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)
  • Concentrations of idarubicin and idarubicinol in nucleated blood and bone marrow cells are more than a hundred times the plasma concentrations. (nih.gov)
  • In this regard, he has identified several markers including FZD9, CD140b, tissue non-specific alkaline phosphatase (TNAP) in combination with other cell surface for prospective isolation of mesenchymal stromal cells (MSCs) from human bone marrow and placenta. (mdanderson.org)
  • Aberrantly expressed cytokines in the bone marrow (BM) niche are increasingly recognized as critical mediators of survival and expansion of leukemic stem cells. (haematologica.org)
  • 2 1 There is growing evidence to suggest that primitive CML cells affect the bone marrow (BM) niche, contributing to deregulated cytokine levels. (haematologica.org)
  • These mice are devoid of endogenous natural killer and T-cell activity and were used to determine whether adoptively transferred expanded natural killer cells could inhibit myeloma growth and myeloma-associated bone destruction. (haematologica.org)
  • The transferred, expanded natural killer cells proliferated in vivo in an interleukin-2 dose-dependent fashion, persisted up to 4 weeks, were readily detectable in the human bone, inhibited myeloma growth and protected bone from myeloma-induced osteolysis. (haematologica.org)
  • Multiple myeloma (MM) is a malignant plasma cell disorder with debilitating symptoms related to anemia, immunosuppression, bone destruction, and renal failure. (haematologica.org)
  • We examined the CD45 dim CD34 + CD38 - CD133 + cells on bone marrow samples of hematologic malignancies and healthy controls using four-color flow cytometry experiments. (researchsquare.com)
  • Interestingly, the CD45 dim CD34 + CD38 - CD133 + cells were highly expressed in bone marrow of patients with AML compared to that of healthy controls (HC). (researchsquare.com)
  • In this study, we focused on measuring LSCs easily in the bone marrow cells from AML patients by developing a four-color flow cytometric analysis. (researchsquare.com)
  • Frequent sites of occurrence of blastic plasmacytoid dendritic cell neoplasm (BPDCN) include the skin, as well as the peripheral blood (PB) and bone marrow (BM). (medscape.com)
  • On bone marrow aspirate smear preparations, the neoplastic cells may display cytoplasmic vacuoles that have been likened to a pearl necklace. (medscape.com)
  • In lymph nodes, the tumor cells infiltrate the interfollicular areas and medullary areas, often diffusely, whereas bone marrow evaluation may show variable degrees of infiltration ranging from sparse to significant. (medscape.com)
  • In all patients that might become considered for intensive treatment, the AML diagnosis should be based on morphologic and immunophenotypic features of bone marrow aspirates as well as on genetic characteristics of the leukemic blasts. (cancercentrum.se)
  • Storage of bone marrow or blood leukemic cells in biobanks is strongly recommended. (cancercentrum.se)
  • We conclude that cytarabine-induced upregulation of the IRF8 in leukemic cells involves increased levels of ZNF224, which can counteract the repressive activity of WT1 on the IRF8-promoter. (lu.se)
  • Both compounds induced up-regulation of proapoptotic BH3-only proteins Bim and Puma, and subsequent cell death. (ashpublications.org)
  • Besides, Dr. Battula is developing novel therapeutic tools to target immune-checkpoint proteins in cancer cells and activate NK/T cells against cancer cells. (mdanderson.org)
  • Autophagy, the type II cell death, is a physiological mechanism involving degradation of intracellular damaged proteins and organelles into membrane vacuoles. (ijpsonline.com)
  • Solid as well as blood cancers are associated with overexpression of HOX proteins in the nucleus, which is traditionally a difficult area of the cell to target with conventional drugs. (nottingham.ac.uk)
  • Sp110 and other NB-associated proteins, induced by type I (α/β) and type II (γ) interferons (IFNs), play a role in IFN response and virus replication [ 8 ]. (biomedcentral.com)
  • Glycans, either alone or complexed with glycan-binding proteins, can deliver intracellular signals or control extracellular processes that promote initiation, execution and resolution of cell death programs. (nature.com)
  • Herein, we review the role of glycans and glycan-binding proteins as essential components of the cell death machinery during physiologic and pathologic settings. (nature.com)
  • At that time, glycobiology, which is the study of carbohydrates and their recognition by motif-specific carbohydrate-binding proteins or lectins, lagged far behind the studies that defined the structural and cellular biology of cell death. (nature.com)
  • In particular, the functional silencing of antigen-experienced T cells, called T cell exhaustion, has become implicated in immune evasion in CLL. (nih.gov)
  • In contrast to T cells, which did not exhibit a significant difference, B cells from PPARγ +/- showed an enhanced proliferative response to stimulation by either lipopolysaccharide or cross-linking of antigen receptors. (jci.org)
  • Mice primed with either ovalbumin or methylated BSA also showed enhanced antigen-specific immune response of both T and B cells, an immunological abnormality that exacerbated antigen-induced arthritis. (jci.org)
  • The neoplastic cells in blastic plasmacytoid dendritic cell neoplasm (BPDCN) are typically positive for CD45, HLA-DR, CD43, CD4, CD56, and cutaneous lymphocyte-associated antigen (CLA). (medscape.com)
  • The pDC markers, CD123 (strong), TCL-1 (90% of cases), CD45RA, blood dendritic cell antigen (BCDA)-2 (expressed in more mature cases), and myxovirus A (MxA) may be detected by flow cytometric immunophenotyping (FCI) and/or immunohistochemical (IHC) staining. (medscape.com)
  • Compared to (2-((2,4,5-trifluorobenzyl)oxy)phenyl)methanol (TFOPM), the oxime derivative TFOBO suppresses leukemic cell growth by significantly increasing reactive oxygen species (ROS) levels and cell death. (bvsalud.org)
  • We recently described that blocking the proton currents in leukemic cells mediated by Hv1 ion channels triggers a marked intracellular acidification and apoptosis induction. (unlp.edu.ar)
  • We isolated primary tumor cells from 13 astrocytoma and oligoastrocytoma patients of all four WHO grades of malignancy and compared the levels of TRAIL-induced apoptosis induction, long-term tumor cell survival, caspase, and caspase target cleavage. (aacrjournals.org)
  • In this study, we demonstrated that Nutlin-3, the small molecule inhibitor of the MDM2/p53 interactions, significantly up-regulated the mRNA and protein levels of α-SMA in normal macrophages as well as in p53(wild-type) but not in p53(mutated/null) myeloid leukemic cells. (unife.it)
  • Regardless of the tumor heterogeneity, cotreatment with the proteasome inhibitor bortezomib efficiently sensitized all primary glioma samples for TRAIL-induced apoptosis and tremendously reduced their clonogenic survival. (aacrjournals.org)
  • The 7-oxysterols also increased the gelatinolytic activity of MMP-9, and a cell-permeable, reversible MMP-9 inhibitor, MMP-9 inhibitor I, significantly impaired sCD14 release. (bvsalud.org)
  • Notably, the role of α-SMA induction in the modulation of myeloid cell migration was clearly documented in α-SMA gene knockdown experiments. (unife.it)
  • More than 15 years ago, cholesterol decrement was first shown to inhibit tumor cell growth, metastasis of tumor cells, and induction of apoptosis (7). (cdc.gov)
  • Therefore, remedies based on the induction of programmed cell death attracted greater attention for cancer treatment [ 8 ]. (ijpsonline.com)
  • Studies have suggested that statins may inhibit tumor cell growth and possibly prevent carcinogenesis. (cdc.gov)
  • The antitumor effect of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in gliomas has thus far only been thoroughly established in tumor cell lines. (aacrjournals.org)
  • DNA damage in human colonic mucosa cells induced by bleomycin and the protective action of vitamin E. (nih.gov)
  • In the present study, we investigated the therapeutic potential of TRAIL in primary human glioma cells. (aacrjournals.org)
  • We established a stable culture model for isolated primary human glioma cells. (aacrjournals.org)
  • Out of the 313 unique human cytokines evaluated, 11 were found to expand cell numbers ≥2-fold in a 7-day culture. (haematologica.org)
  • In this research, we hypothesized that Sp110 may be involved in the infection of human promyelocytic HL-60 cells with A. phagocytophilum . (biomedcentral.com)
  • The human Sp110 and A. phagocytophilum msp4 mRNA levels were evaluated by real-time RT-PCR in infected human HL-60 cells sampled at 0, 12, 24, 48, 72 and 96 hours post-infection. (biomedcentral.com)
  • The A. phagocytophilum infection levels were evaluated in HL-60 cells after RNAi by real-time PCR of msp4 and normalizing against human Alu sequences. (biomedcentral.com)
  • In the laboratory, A. phagocytophilum can be propagated in undifferentiated human promyelocytic HL-60 cells. (biomedcentral.com)
  • In contrast to cell lines, isolated primary tumor cells from all investigated glioma patients were highly TRAIL resistant. (aacrjournals.org)
  • Dr. Battula's research is also focused on targeting cancer stem cells (CSCs), a rare population of cells present in the primary tumor that are drug resistant and primary cause of disease relapse. (mdanderson.org)
  • A successful therapy must therefore eliminate these cells known to be highly resistant to apoptosis. (hindawi.com)
  • FLT3 (FMS-like tyrosine kinase 3) is a type III receptor tyrosine kinase (RTK) closely related to the platelet-derived growth factor (PDGF) receptor and c-Kit with important functions in the regulation of early hematopoietic cells. (ashpublications.org)
  • 1 The BCR-ABL1 fusion protein is a constitutively active tyrosine kinase and triggers a cascade of aberrant downstream signaling pathways leading to clonal outgrowth of CML cells and subsequent disease manifestation. (haematologica.org)
  • What is the precise role of intracellular and extracellular galectins in the control of cell death programs? (nature.com)
  • A diffuse infiltrate of medium-sized cells with dispersed chromatin ("blastic") is characteristic of blastic plasmacytoid dendritic cell neoplasm (BPDCN). (medscape.com)
  • [ 11 ] In the skin, BPDCN cells infiltrate the dermis without significant epidermotrophism. (medscape.com)
  • Adenine improves the survival of rat Purkinje cells and enhances the storage of erythrocyte in whole blood [ 6 - 8 ]. (hindawi.com)
  • Additionally, the high levels of the CD45 dim CD34 + CD38 - CD133 + cells in AML patients were an independently significant poor risk factor for overall survival and event free survivals. (researchsquare.com)
  • Also, these cell populations directly correlated with poor survival [ 2 , 5 ]. (researchsquare.com)
  • Although α-SMA is often expressed in stromal cells associated to a variety of cancers including hematological malignancies, up to now the role of anti-cancer drugs on α-SMA has not been deeply investigated. (unife.it)
  • An established characteristic of neoplastic cells is their metabolic reprogramming, known as the Warburg effect, with greater reliance on energetically less efficient pathways (such as glycolysis and pentose phosphate shunt) compared with oxidative phosphorylation. (unlp.edu.ar)
  • Here, we first tested a novel oxime-containing derivative of 2-((2,4,5-trifluorobenzyl)oxy)benzaldehyde oxime (TFOBO) to evaluate its anticancer effect in myeloid leukemic cells. (bvsalud.org)
  • In the present study, we investigated the anticancer effects of adenine and the underlying mechanism in colon cancer cells. (hindawi.com)
  • Recently, adenine has been explored for its anticancer property in several types of cancer cell lines. (hindawi.com)
  • We explored the possible mechanisms underlying the anticancer activity of adenine in colorectal cancer cells. (hindawi.com)
  • In conclusion, the current investigation regarding anticancer effects of piperine against breast cancer revealed remarkable suppression of cancer cells. (ijpsonline.com)
  • [ 4 , 5 ] Previously used terms for this disorder have included CD4+CD56+ hematodermic tumor and blastic natural killer (NK) cell lymphoma. (medscape.com)
  • As4O6 induced apoptosis in U937 leukemic cells at much lower concentrations than As2O3 via an early increase of cellular reactive oxygen species (ROS), and a decrease in cellular mitochondrial membrane potential, followed by cytochrome c release and caspase-3 activation. (spandidos-publications.com)
  • As4O6 generated ROS and induced caspase-3 activation more potently than As2O3 in U937 cells. (spandidos-publications.com)
  • CD40 ligand is an accessory signal for T-cell activation and can overcome T-cell anergy. (houstonmethodist.org)
  • We show that T cells express high levels of the inhibitory exhaustion markers programmed cell death 1 (PDCD1, also termed PD-1) and lymphocyte-activation gene 3 (LAG3), whereas CLL cells express high levels of CD274 (also termed PD-ligand 1). (nih.gov)
  • However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
  • In summary, we identify myostatin propeptide as a novel positive regulator of primitive CML cells and corresponding normal hematopoietic cells. (haematologica.org)
  • Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2 cells, in a dose-dependent manner. (hindawi.com)
  • Cell migration as well as cell invasive ability of MCF-7 cells was reduced to minimum by the application of piperine in dose dependent-manner. (ijpsonline.com)
  • CD40L or OX40L alone was insufficient to expand tumor-reactive T cells. (houstonmethodist.org)
  • The combination of CD40L and OX40L on B-CLL cells may allow the generation of therapeutic immune responses to B-CLL, either by active immunization with modified tumor cells or by adoptive immunotherapy with tumor-reactive autologous T cells. (houstonmethodist.org)
  • The group are currently focused on the pivotal role that HOXA cluster genes play in the demarcation and possible emergence of haematopoietic stem cells (HSCs) and their role in cancer. (nottingham.ac.uk)
  • Pathobiology: Cells and Genes;Cellular and Molecular Haematology. (nottingham.ac.uk)
  • Cell viability was measured using the MTT assay. (hindawi.com)
  • 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was executed for determination of effects of piperine on breast cancer cell viability. (ijpsonline.com)
  • Although one typically thinks of carbohydrates as associated with cell growth and viability, glycosylation also has an integral role in many processes leading to cell death. (nature.com)
  • In the present study we have evaluated the inhibitory effect of three non-toxic antiviral natural compounds namely berberine, curcumin or picroliv against FMuLv induced erythroleukemia in BALB/c mice. (oldcitypublishing.com)
  • Mutations in the Neuraminidase-Like Protein of Bat Influenza H18N11 Virus Enhance Virus Replication in Mammalian Cells, Mice, and Ferrets. (cdc.gov)
  • Both NMDA and kainate also induced rapid increases in neuronal [Zn 2+ ] i . (jneurosci.org)
  • The kainate-induced increase was highly sensitive to Gd 3+ or Na + removal in most neurons but insensitive in a minority subpopulation ("cobalt-positive cells"), suggesting that a fourth route of neuronal Zn 2+ entry is through the Ca 2+ -permeable channels gated by certain subtypes of AMPA or kainate receptors. (jneurosci.org)
  • We then outline preclinical approaches that employ CAR-NK cells for GB immunotherapy, and give an overview on the ongoing clinical development of ErbB2 (HER2)-specific CAR-NK cells currently applied in a phase I clinical trial in glioblastoma patients. (frontiersin.org)
  • Development of pre-clinical models using stem cell technologies. (nottingham.ac.uk)
  • The 2016 World Health Organization (WHO) classification of lymphoid neoplasms incorporates genetic data, clinical features, cell morphology, and immunophenotype, all of which have important implications for disease prognosis and management. (msdmanuals.com)
  • [ 11 ] Although only minimal or low-level peripheral blood involvement is usuallly seen at presentation, most cases eventually evolve into an overt leukemic phase. (medscape.com)
  • This phase is characterized by poor control of the blood counts with myelosuppressive medication and the appearance of peripheral blast cells (≥15%), promyelocytes (≥30%), basophils (≥20%), and platelet counts less than 100,000 cells/μL unrelated to therapy. (medscape.com)
  • In contrast to normal cells, most cancer cells predominantly produce energy by a high rate of glycolysis followed by lactate fermentation, even in the presence of oxygen, a less efficient metabolism compared to a low rate of glycolysis followed by mitochondrial oxidation of pyruvate [ 2 ]. (hindawi.com)
  • The hematopoietic tumor suppressor interferon regulatory factor 8 (IRF8) is upregulated by the antimetabolite cytarabine in leukemic cells involving the zinc finger protein ZNF224, acting as a cofactor of the Wilms' tumor gene 1 (WT1) protein. (lu.se)
  • In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)