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  • vitro
  • In this study, we investigated the potential application of this first-in-class inhibitor MLN4924 in the treatment of human renal cell carcinoma both in vitro and in vivo. (deepdyve.com)
  • One candidate for GBM therapy is Resveratrol (Rsv), which has additive toxicity with TMZ in several glioma cells in vitro and in vivo. (ufrgs.br)
  • In conclusion, WT1 pTj peptide entered cancer cells and promoted antitumor activity in vitro and in vivo. (aacrjournals.org)
  • Our in vitro and in vivo results demonstrated that hPTTG1 overexpression reinforces senescence through CXCR2 signaling, and the evasion of CXCR2/p21-dependent senescence was critical to hPTTG1 exerting its oncogenic potential. (biomedcentral.com)
  • Among several attempts initiated to decrease cardiotoxic adverse effect of this valuable drug, scavenging of ROS by natural antioxidants demonstrates favorable cardioprotective effect against DOX-induced cardiotoxicity both in vitro and in vivo [ 4 , 5 ]. (hindawi.com)
  • Compared to the cardiotoxicity of Trichostatin A in vitro, single or cumulative melatonin treatment induced insignificant detrimental effects on neonatal cardiomyocytes. (drcalapai.net)
  • Overexpression
  • Deciphering the mechanism by which hPTTG1 overexpression induces these contradictory actions in breast cancer cells is critical to our understanding of the role of hPTTG1 in breast cancer development. (biomedcentral.com)
  • The interplay between hPTTG1 overexpression and chemokine C-X-C motif receptor 2 (CXCR2)/p21-dependent senescence in tumor growth and metastasis of MCF-7 cells was investigated by orthotopic transplantation of severe combined immunodeficiency (SCID) mice. (biomedcentral.com)
  • Herein, we showed that hPTTG1 overexpression reinforced senescence through the CXCR2/p21 signaling. (biomedcentral.com)
  • Furthermore, hPTTG1 overexpression activated NF-κB signaling to transactivate the expression of interleukin (IL)-8 and growth-regulated oncogene alpha (GROα) to execute CXCR2 signaling in MCF-7 cells. (biomedcentral.com)
  • Differentiation
  • Mesenchymal stem cells (MSCs) have attracted great interest as a promising regenerative therapeutic for many human diseases, primarily because of their capacity for self-renewal, multilineage differentiation, and immune modulation [ 9 - 13 ]. (hindawi.com)
  • MSCs have a limited lifespan in cell culture, and after a few passages of expansion, the cells enter the senescent state, leading to the reduction in self-renewal ability and differentiation potential [ 18 , 19 ]. (hindawi.com)
  • Results TBI reduced the pool of BM mesenchymal stem/progenitor cells, and altered osteoblast differentiation ability of BM MSC, evidenced by changes in TAZ expression. (haematologica.org)
  • In addition to the roles of HSC, the initiation and maintenance of hematopoiesis also depends on the hematopoietic microenvironment or niche, which ensures the size of the stem cell pool and regulates the differentiation of HSC. (haematologica.org)
  • Genes
  • Lundberg AS, Hahn WC, Gupta P, Weinberg RA (2000) Genes involved in senescence and immortalization. (edu.pl)
  • Proapoptotic, antiapoptotic and senescence genes, as well as HPV-E6/E7 mRNA expression, were detected by time real RT-PCR. (biomedcentral.com)
  • reactive
  • The redox recycling of semiquinone and its parent quinone is known to generate reactive oxygen species (ROS) leading to mitochondria dysfunction, myocyte senescence, and apoptosis, and ultimately causing cardiac remodeling and contractility impairment [ 2 , 3 ]. (hindawi.com)
  • secretome
  • To gain insight into the regulation of IGFBP3-induced senescence, we undertook a proteomic screening for IGFBP3 interactor(s) in the secretome and identified t-PA as candidate interactor. (aacrjournals.org)
  • molecular
  • Although the specific molecular mechanisms remain to be defined, it is presumed that aging of cardiac stem or progenitor cells may contribute to the loss of regenerative potential. (hindawi.com)
  • Our findings provide novel molecular insights into hPTTG1-induced senescence and identify a novel mechanism by which hPTTG1 promotes metastasis by regulating the senescence-associated microenvironment. (biomedcentral.com)
  • Jolly C, Morimoto RI (2000) Role of the heat shock response and molecular chaperones in oncogenesis and cell death. (edu.pl)
  • Moreover, they contribute to the identification of specific molecular targets whose modulation may prevent age-associated cell dysfunction and improve human healthspan. (hindawi.com)
  • The Proceedings will be useful for cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and other disciplines related to animal cell culture, working either in academic environments or in industries of biotechnology and pharmacy. (booktopia.com.au)
  • mechanism
  • The radiation induced DNA damage of tumour cells", explained Tortosa, "the cells activate repairing mechanisms and, if they are unable to repair, they destruct themselves (a mechanism known as apoptosis). (healthcanal.com)
  • However, the mechanism of Rsv and TMZ additive toxicity, which is the aim of the present work, is not clear, especially concerning cell cycle dynamics and long term effects. (ufrgs.br)
  • MCF-10A and MCF-7 cells were used to identify the mechanism of hPTTG1-induced senescence. (biomedcentral.com)
  • secretion
  • To identify such mediator(s), we undertook a quantitative proteomic analysis of the protein secretion from senescent MCF-7 cells and observed significantly increased levels of secreted IGFBP3. (aacrjournals.org)
  • The cytokine antibody array showed the highest secretion levels of IL-6 and CXCL1 in NOFs co-cultured with OSCC cells. (pubtransformer.com)
  • Tumour
  • With Nutlin-3a we have seen that increases tumour cell death and therefore increases the effectiveness of radiotherapy treatment. (healthcanal.com)
  • proteins
  • The cell cycle regulatory mol- ecules, apoptosis-related molecules, and cell stress-related proteins were examined by Western blotting. (deepdyve.com)
  • Senescent cells release a wide variety of factors, including extracellular domains of transmembrane proteins that require proteolytic cleavage by specific proteases. (k-state.edu)
  • ADAMs (A Disintegrin and Metalloprotease domain-containing proteins) are enzymes that cleave many transmembrane proteins, such as growth factor precursors or adhesion molecules, and thus may act as sheddases in senescent cells. (k-state.edu)
  • Both high glucose treatments increased the expressions of p16 and pRB proteins in young rat AF cells for one and three days. (springermedizin.de)
  • However, compared to normal control, the expressions of p53 and p21 proteins were decreased in young rat AF cells treated with both high glucoses for one and three days. (springermedizin.de)
  • We treated HCT116 human colorectal adenocarcinoma cells with 10 μm melatonin and determined the levels of cell death-related proteins and evaluated cell cycle kinetics. (drcalapai.net)
  • The goal of animal cell technology includes accomplishments of clonal expansion of differentiated cells with useful ability, optimization of their culture conditions, modulation of their ability for production of medically and pharmaceutically important proteins, and the application of animal cells to gene therapy and artificial organs. (booktopia.com.au)
  • upregulation
  • Consistent with its growth-suppressive effects, HIF-1α was important for upregulation of the cell cycle inhibitors p21 (CDKN1A) and p27 (CDKN1B). (ovid.com)
  • viability
  • Park EY, Park JB (2013) Dose- and time-dependent effect of high glucose concentration on viability of notochordal cells and expression of matrix degrading and fibrotic enzymes. (springermedizin.de)
  • Exposure to 100 μM BPA had no effects on cell viability, but increased cytoplasmic expression of ERβ and release of GDF-15, as well as decreased release of IL-6, ET-1, and IP-10 through suppression of NFκB phosphorylation. (carleton.ca)
  • retinal
  • Requirement of NOX2 expression in both retina and bone marrow for diabetes-induced retinal vascular injury," PLoS ONE , vol. 8, no. 12, Article ID e84357, 2013. (hindawi.com)
  • results
  • Moreover, prolonged drug incubation (up to 168h) resulted in massive detachment of cells from culture plates, which together with Annexin V/PI results, indicated that necrosis was the main response of HT29 cells to C-1748 treatment. (biomedsearch.com)