• In SARS-CoV-2 (COVID-19) infected Caco-2 cells, the phosphorylase activity of CK2 is increased resulting in phosphorylation of several cytoskeletal proteins. (wikipedia.org)
  • Once recruited to DSB, these complexes get activated and induce the phosphorylation of numerous targets including transducing kinases, which subsequently phosphorylate downstream effectors to delay cell cycle and promote DNA repair. (cea.fr)
  • Additionally, the checkpoint kinases modify the chromatin surrounding DNA damages through phosphorylation of the H2A histone (H2AX in mammals). (cea.fr)
  • regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. (proteopedia.org)
  • Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. (proteopedia.org)
  • Compound 51 inhibited the proliferation of 13 out of 15 cancer cell lines with IC50 values between 0.27 and 6.9 muM, which correlated with the complete suppression of retinoblastoma phosphorylation and the onset of apoptosis. (proteopedia.org)
  • Cdk phosphorylation triggers sequential intramolecular interactions that progressively block Rb functions as cells move through G1. (proteopedia.org)
  • 12 The activation of cyclin/CDK complex accelerates cell cycle progression, which are regulated by phosphorylation. (jcpjournal.org)
  • Cuc B induced phosphorylation of Chk1 on Ser-345, phosphorylation of Cdc25C on Ser-216, and phosphorylation p53 on Ser-15 had been all inhibited by ATM knockdown (Fig. 4C). (achrinhibitor.com)
  • Additionally, Cuc B brought on phosphorylation of your Chk1 downstream effector Cdc25C on Ser-216 and Cdk1 on Tyr15 had been also inhibited (Fig. 5C).Cuc B induced ROS generation and didn't influence STAT3 phosphorylationRecent research have shown that Cuc B induced intracellular ROS formation in HeLa, SW480, and B16F10 cells [14,15,39]. (achrinhibitor.com)
  • As local cell density increases, tensile forces on E-cadherin adhesions are reduced, which prompts the accumulation of the G2 checkpoint kinase Wee1 and downstream inhibitory phosphorylation of Cdk1. (ibecbarcelona.eu)
  • PIG3 knockdown led to an abnormal DNA damage response, including decreased IR-induced phosphorylation of H2AX, Chk1, Chk2 and Kap-1 as well as a prolonged G2-M arrest and aberrant mitotic progression. (ijbs.com)
  • J-H Lee et al also have provided the first evidence showing that PIG3 is closely associated with the induction and maintenance of the phosphorylation of H2AX and some other DDR proteins after DNA damage induced by NCS ( 8 ). (ijbs.com)
  • We find that together with loss-of-function mutations in p53 and, to some degree, potentiated by cMyc overexpression, Dusp4 deletion aids in cell cycle checkpoint escape while simultaneously potentiating hallmarks of replicative stress, including multinucleation and Chk1 phosphorylation. (biomedcentral.com)
  • Here, we demonstrate that the S phase checkpoint protein CHK1 is maintained in a low phosphorylation state in Spi1/PU.1-overexpressing cells and provide evidence that this is not due to negative control of its primary kinase ATR. (oncotarget.com)
  • 24 hours) results in a large shift in cell-cycle populations from G1 and G2-M to S-phase with an accompanied induction of H2AX phosphorylation in U-2 OS cells [1] . (medchemexpress.com)
  • Having roles in cell cycle regulation may also indicate CK2's role in allowing cell cycle progression when normally it should have been ceased. (wikipedia.org)
  • Cyclin-dependent kinases (CDKs) are serine/threonine protein kinases that act as key regulatory elements in cell cycle progression. (proteopedia.org)
  • Here, we show that epithelial cells sense local cell density through mechanosensitive E-cadherin adhesions to control G2/M cell-cycle progression. (ibecbarcelona.eu)
  • Thus, WEE1 inhibition sensitizes cancers have loss-of-function TP53 mutations, meaning that tumor cells to DNA-damaging chemotherapy and can lead to they become more dependent on the G2/M- and S-phase unstable DNA replication, DNA damage and mitotic catas- checkpoints to halt progression of the cell cycle [3, 4]. (sagepub.com)
  • In the presence of errors or damage during DNA replication, cell cycle checkpoint nodes and repair machinery work in concert to retard cell cycle progression until sufficient repair has been achieved. (bmj.com)
  • By exogenously modulating its activity, we demonstrate that PP1α is required to maintain CHK1 in a dephosphorylated state and, more importantly, that it is responsible for the accelerated replication fork progression in Spi1/PU.1-overexpressing cells. (oncotarget.com)
  • Casein kinase 2 (EC 2.7.11.1)(CK2/CSNK2) is a serine/threonine-selective protein kinase that has been implicated in cell cycle control, DNA repair, regulation of the circadian rhythm, and other cellular processes. (wikipedia.org)
  • Cell-cycle arrest was associated with the engagement of checkpoint kinase 2-cell division cycle 25C-cyclin-dependent kinase 1/cyclin B1 signaling. (bioone.org)
  • EESR induced cell cycle arrest at G2/M phase in a dose-dependent manner by modulating cyclin B, cyclin-dependent kinase 1 (CDK1), and CDK inhibitor p21 expression. (jcpjournal.org)
  • 8 , 9 Cell cycle program is regulated by cyclin/cyclin-dependent kinase (CDK) complexes. (jcpjournal.org)
  • Long term and extreme ER tension induced -cell apoptosis is certainly connected with c-jun N-terminal kinase (JNK) activation [9], [15]. (cgp60474.com)
  • The presence of replication stress activates the DNA damage response and downstream checkpoint proteins including ataxia telangiectasia and Rad3 related kinase (ATR), checkpoint kinase 1 (CHK1), and WEE1-like protein kinase (WEE1), which trigger cell cycle arrest while protecting and restoring stalled replication forks. (bmj.com)
  • This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. (cancerindex.org)
  • L5178Y is often used in mouse lymphoma L5178Y cell Tk (thymidine kinase) gene mutation assays. (biomedcentral.com)
  • Some studies have reported that the G2 M arrest induced by SP600125 may be due to inhibition of cyclin B Cdk1 kinase activity through an increase in p21 levels. (hsp90-inhibitors.com)
  • Indeed in S and G2 phase, RPA binding to 3' single strand overhangs facilitates the recruitment of proteins of the Rad52 epistasis group, among which Rad51, which carries out the strand-exchange reaction. (cea.fr)
  • It seems now clear that posttranslational modification of both DNA repair and checkpoint proteins is of importance for the regulation of their activities but how these modifications are regulated and how they affect the activity of the proteins only begins to be described. (cea.fr)
  • Proteomics identified the 5925 proteins in total, and the significantly upregulated proteins in CSC compared with FCS-induced differentiated CSC and its parental cells were extracted. (bvsalud.org)
  • Thus, baicalein modulates the expression of DNA damage response proteins and G2/M phase regulatory molecules. (tku.edu.tw)
  • ATF4 activates the transcription of C/EBP homologous proteins (CHOP), considered to mediate palmitate-induced -cell loss of life [10], [11]. (cgp60474.com)
  • Dynamic IRE1 splices X-box binding proteins-1 (Xbp)-1 mRNA, translating into a dynamic transcription aspect sXbp-1 that induces ER chaperones and ER-associated proteins degradation. (cgp60474.com)
  • Each origin is initiated by a combination of regulatory proteins that prepare the chromatin for replication before synthesis (S)-phase entry. (bmj.com)
  • Any obstacles encountered by cells in this process can lead to 'replicative stress' ( Figure 1 ), 1 which may be overcome by replicative stress response proteins, but deficiencies in this response result in accumulated errors in DNA replication and loss of genomic integrity, which lead to cell death. (bmj.com)
  • CK2 has a dual functionality with involvement in cell growth/proliferation and suppression of apoptosis. (wikipedia.org)
  • Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
  • Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
  • JAM3 knockdown additionally inhibited trophoblast proliferation and increased the number of trophoblasts in the sub-G1 and G2/M phases, indicating cell-cycle disturbance and apoptosis. (bioone.org)
  • Binding of one molecule of eribulin to two microtubules can inhibit cell proliferation by 50%, and such binding is reversible. (shu.edu)
  • Knockdown of HMGA1 in KKU-055-CSC suppressed the expression of stem cell markers, induced the differentiation followed by cell proliferation, and enhanced sensitivity to chemotherapy drugs including Aurora A inhibitors. (bvsalud.org)
  • Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). (proteopedia.org)
  • Inhibitory effect of EESR on cancer cell growth and proliferation was determined by water-soluble tetrazolium salt assay. (jcpjournal.org)
  • Exposure to benzo(a)pyrene affected the expression of genes that are involved in cell cycle regulation in both genotypes, indicating that the presence of unrepaired DNA damage in testis blocks cell proliferation to protect DNA integrity in both DNA repair proficient and deficient animals. (maastrichtuniversity.nl)
  • Related to its effect on other reported cancer cells, Cuc B could considerably inhibit A549 cells proliferation and development inside a dose- and time- dependent manner. (achrinhibitor.com)
  • Although low concentrations of Cuc B showed no important impact on A549 cell proliferation right after 24 h therapy, prolonged treatment significantly inhibited cancer cells proliferation and colony formation clearly demonstrating that Cuc B is often a potent cytotoxic compound. (achrinhibitor.com)
  • Under experimental normoxic condition (21 % O 2 ), deferasirox (DFX) used at 10 μM for 3 days reduced proliferation, led cell cycle arrest in S and G2-M phases and induced cytotoxicity and apoptosis in U251 and U87 GBM cells. (biomedcentral.com)
  • Irradiation at a dose of 16 Gy repressed proliferation, cytotoxicity and apoptosis, but only in U251 cells, while no synergy with DFX was observed in either cell line. (biomedcentral.com)
  • The anticancer activities of iron depletion are based on the fact that neoplastic cells require more iron than normal cells for proliferation [ 2 ]. (biomedcentral.com)
  • The current study demonstrated that PD markedly inhibited PAC cell proliferation and triggered their apoptosis. (biomedcentral.com)
  • These results indicate that JNK activity may regulate cell proliferation through the regulation of cell cycles. (hsp90-inhibitors.com)
  • When added with other potent anti-cancer therapies, a CK2 inhibitor may increase the effectiveness of the other therapy by allowing drug-induced apoptosis to occur at a normal rate. (wikipedia.org)
  • In these same cells, the CK2 inhibitor silmitasertib displayed potent antiviral activity. (wikipedia.org)
  • 13 The expression of CDK inhibitor p21 is upregulated by activated p53, resulting in the suppression of G2/M transition by the inactivation of cyclin/CDK complex. (jcpjournal.org)
  • 13 So, in one,25D resistant 40AF cells HPK1 doesn't seem inhibitor checkpoint inhibitors to signal differentiation with the JNK pathway. (cox2-inhibitors.com)
  • The pan caspase inhibitor Q VD OPh even more enhances DCS induced differentiation of 40AF cells by inhibition of HPK1 cleavage. (cox2-inhibitors.com)
  • Here we tested whether roscovitine, an inhibitor of cyclin-dependent kinases (CDKs), which hereby also blocks homologous recombination (HR), can be used to enhance the radiation sensitivity of HNSCC cell lines. (oncotarget.com)
  • Due to a defect in HR, all HPV positive cell lines were efficiently radiosensitized by the PARP-1 inhibitor olaparib. (oncotarget.com)
  • The study incorporates disciplines such as Cancer research and Topoisomerase-I Inhibitor in addition to Cell cycle checkpoint. (research.com)
  • The concepts of his DNA damage study are interwoven with issues in CHEK1, Cell cycle checkpoint, DNA repair, Regulator and Topoisomerase-I Inhibitor. (research.com)
  • His work carried out in the field of DNA damage brings together such families of science as CHEK1, Cell cycle checkpoint, Cell killing, Cell biology and Topoisomerase-I Inhibitor. (research.com)
  • Farnesyltransferase inhibitor tipifarnib is well leurocristine tolerated, induces stabilization of disease, and inhibits farnesylation and oncogenic tumor survival pathways in patients with advanced multiple myeloma. (hsp90-inhibitors.com)
  • Previous data suggested that Cuc B triggered cell cycle arrest by blocking the STAT3 signaling pathway, which resulted in reduced expression of downstream targets, like Cyclin B1, Cyclin A [402]. (achrinhibitor.com)
  • Activation of STAT3 signaling could up-regulate Cyclin B1, c-Myc, Bcl-x and regulating cell growth and survival.Chk1 knockdown reversed Cuc B induced G2/M phase arrestTo dissect the downstream effector in Cuc B mediated G2/M phase arrest, the part of Chk1 was examined with Chk1 siRNA. (achrinhibitor.com)
  • Given the previously reported role of DUSP4 as a p53 target, a potential cell cycle checkpoint, and in mediating senescence downstream of replication stress, we hypothesized that DUSP4 may be a critical oncogenic driver in breast cancer. (biomedcentral.com)
  • Furthermore, we established a BCa cell model with downregulated LAPTM5, revealing a significantly delayed growth rate in the BCa cells with knockdown of LAPTM5. (spandidos-publications.com)
  • JAM3 knockdown induced reactive oxygen species and syncytin 2 expression in trophoblasts. (bioone.org)
  • PIG3 knockdown can suppress intra-S phase and G2/M checkpoints ( 8 ). (ijbs.com)
  • We therefore asked what role JMJD1B plays in newly synthesized histone maturation by exploiting the knockdown of JMJD1B by treating HeLa cells with siRNA against JMJD1B (siJMJD1B) and observed the reduction of both JMJD1B protein on cytosolic extracts (Fig. 1 b) and mRNA levels (Fig. 1 c) 72 h after the treatment. (biomedcentral.com)
  • 10 , 11 The major molecules responsible for G2/M transition are cyclin A /CDK1 and cyclin B/CDK1. (jcpjournal.org)
  • To test the effect of ATM siRNA on Cuc B induced Cdk1 and Cyclin B1 interactions, IP was performed. (achrinhibitor.com)
  • Within this study, we showed that Cuc B induced G2/M arrest inside a ROS dependent manner without having affecting STAT3 in A549 cells: Cuc B induced ROSmediated DNA harm, which activated G2/M phase checkpoint through ATM-activated Chk1-Cdc25C-Cdk1 and -p53-14-3-3-s cascades. (achrinhibitor.com)
  • The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle. (wuxibiortus.com)
  • In all five HPV negative and HPV positive cell lines tested, roscovitine caused inhibition of CDK1 and 2. (oncotarget.com)
  • Following DNA damage, WEE1 inhibits CDK1, leading 1 Introduction to cell cycle arrest and allowing time for DNA repair [4]. (sagepub.com)
  • Thus, inhibition of JNK activity prevents dissociation between p21 and JNK, and then prevents inhibition of cyclin B Cdk1 activity, leading to induction of G2 M arrest. (hsp90-inhibitors.com)
  • Immunotherapy mainly includes immune checkpoint inhibitors (ICIs), such as inhibitors of PD-1 (programmed cell death 1)/programmed cell death ligand 1 (PD-L1). (nature.com)
  • Increasing evidence indicates that senescent cells could be a promising new target for therapeutic intervention known as senotherapy, which includes depleting senescent cells, modulating SASP and restoration of senescence inhibitors. (frontiersin.org)
  • Inducing cell cycle arrest by cucurbitacins has been B7-2/CD86 Inhibitors targets properly Methyl-PEG3-Ald custom synthesis established while the detailed mechanisms and pathways are largely to become clear. (achrinhibitor.com)
  • Blockade of CHK2 activation by CHK2 inhibitors protects cells from baicalein-mediated G2/M cell cycle arrest. (tku.edu.tw)
  • Small molecule inhibitors designed to target the DNA damage sensors, such as inhibitors of ataxia telangiectasia-mutated (ATM), ATR, CHK1 and WEE1, impair smooth cell cycle modulation and disrupt efficient DNA repair, or a combination of the above, have demonstrated interesting monotherapy and combinatorial activity, including the potential to reverse drug resistance and have entered developmental pipelines. (bmj.com)
  • This review examines how LSD1 modulates different cell behavior in TME and emphasizes the potential use of LSD1 inhibitors in combination with ICB therapy for future cancer research studies. (biomedcentral.com)
  • Comparable to that of ATM siRNA, Cuc B- induced G2/M arrest in A549 cells was substantially decreased by Chk1 siRNA therapy (Fig. 5A, 5B). (achrinhibitor.com)
  • Notably, we found that the expression of the CHK1 phosphatase PP1α is increased in Spi1/PU.1-overexpressing cells. (oncotarget.com)
  • pre-treated for 15 minutes) inhibits CHK1 autophosphorylation (S296) and CHK2 autophosphorylation (S516) in HT-29 cells [1] . (medchemexpress.com)
  • Inhibited CHK1 autophosphorylation (S296) and CHK2 autophosphorylation (S516) (IC50 of less than 31 nM) in HT-29 cells. (medchemexpress.com)
  • To examine the mechanism by which DCS reverses resistance of 40AF buy Roscovitine cells to 1,25D, we asked if HPK1 sig naling is enhanced from the inhibition of its proteolytic cleavage, regarded to take area in other methods. (cox2-inhibitors.com)
  • Here, the results show that baicalein (5,6,7-trihydroxyflavone) treatment inhibits the growth of highly invasive ovarian cancer cells, and that baicalein-induced growth inhibition is mediated by the cell cycle arrest in the G2/M phase. (tku.edu.tw)
  • Inhibition of glutathione synthesis, glutathione S-transferase (GST) activity, and multidrug resistance protein (MRP) transporter function attenuated arsenite resistance, consistent with studies suggesting that arsenite is extruded from the cell as a glutathione conjugate by MRP-1. (uky.edu)
  • The tumor suppressor protein p53 is inhibition also exploits the G1 checkpoint deficiency seen involved in regulation of the G1 checkpoint. (sagepub.com)
  • for three cycles) causes growth inhibition in tumor xenografts [1] . (medchemexpress.com)
  • We also observed that PIG3 formed specific ionizing radiation-induced foci (IRIF) colocalized with γ-H2AX foci and partially with pKAP-1( 7 ). (ijbs.com)
  • Interestingly, replication stress in Brca2-null cells activates p53 and the expression of its target genes, including senescence-inducing Ink4/Arf. (biomedcentral.com)
  • 0.5 to 9 hours) induces replication stress and depletes the pool of available RPA2 for binding to DNA [1] . (medchemexpress.com)
  • The current study examines factors influencing sensitivity to arsenic using human malignant melanoma cell lines. (uky.edu)
  • Impairment of a cell cycle checkpoint is often associated with sensitivity to chemotherapeutic drugs. (bmj.com)
  • Then we found that restored expression of CHFR by demethylation decreased the sensitivity to paclitaxel in AN3CA cells. (bmj.com)
  • Therefore, we hypothesized that clastogens cause NS in cultured mammalian cells, since the mouse lymphoma L5178Y cell line is known to have a similar sensitivity to clastogens. (biomedcentral.com)
  • Therefore, elimination of the checkpoint increases the sensitivity of human tumor cell lines to anticancer agents. (hsp90-inhibitors.com)
  • Junctional adhesion molecule 3 (JAM3) is involved in epithelial cell junction, cell polarity, and motility. (bioone.org)
  • Epithelial cell divisions are coordinated with cell loss to preserve epithelial integrity. (ibecbarcelona.eu)
  • EESR showed significant antioxidant activity and inhibitory effect on HT29 cell growth in a dose-dependent manner. (jcpjournal.org)
  • This can be consistent with our previ ously reported observation that JNK2 action is inhibitory to differentiation of 40AF cells. (cox2-inhibitors.com)
  • All the results suggest that baicalein has another novel growth inhibitory effect on highly invasive ovarian cancer cells, which is partly related to G2/M cell cycle arrest through the ROS-mediated DNA breakage damage and CHK2 activation. (tku.edu.tw)
  • EESR-induced apoptosis was associated with the upregulation of p53, a death receptor Fas, and a pro-apoptotic protein Bax and the activation of caspase 3, 8, and 9, resulting in the degradation of PARP. (jcpjournal.org)
  • Baicalein-induced G2/M phase arrest is associated with an increased reactive oxygen species (ROS) production, DNA damage, and CHK2 upregulation and activation. (tku.edu.tw)
  • This study identifies a novel mechanism for breast tumorigenesis implicating Dusp4 loss and p53 mutations in cellular acquisition of Dbf4 upregulation as a driver of cellular replication and cell cycle checkpoint escape. (biomedcentral.com)
  • Therefore, the upregulation of p21 expression may contribute to G2 M arrest in the early stages, and then Cdk2 may regulate endoreduplication by treating leukemia cells in the middle stages in the presence of SP600125. (hsp90-inhibitors.com)
  • One such mechanism involves the upregulation of immune checkpoints, such as programmed death-1 (PD-1)/programmed death-ligand 1 (PD-L1), cluster of differentiation 86 (CD86)/cytotoxic T-lymphocyte antigen 4 (CTLA-4) [ 16 ]. (biomedcentral.com)
  • De-regulation of CK2 has been linked to tumorigenesis as a potential protection mechanism for mutated cells. (wikipedia.org)
  • Eribulin exerts its effects via a tubulin-based antimitotic mechanism leading to G2/M cell-cycle block, disruption of mitotic spindles, and, ultimately, apoptotic cell death after prolonged mitotic blockage. (shu.edu)
  • Centrosome duplication is tightly regulated and limited at once per cell cycle through a mechanism that prevents re-duplication. (biomedcentral.com)
  • The objective of this study is to evaluate the antioxidant and anticancer effects of ethanol extract of S. rufopilosa (EESR) and to determine the molecular mechanism of its anticancer activity in human colon carcinoma HT29 cells. (jcpjournal.org)
  • In the present study, the potential mechanism of PIG3 participation in the DNA damage response induced by ionizing radiation (IR) was investigated in multiple cell lines with depleted expression of PIG3 transiently or stably by the small interference RNA and lentivirus-mediated shRNA expression strategies. (ijbs.com)
  • Thus, we are able to better understand the molecular hereditary mechanism of poultry stress-induced immunosuppression. (moonphase2018.com)
  • We had also found that ERK1c translocates to the Golgi at the G2 to M phase transition, but the molecular mechanism underlying this recruitment remains unknown. (biologists.com)
  • These cells are readily triggered to divide following epithelial wounding due to the consequent increase in intercellular forces and resulting degradation of Wee1. (ibecbarcelona.eu)
  • WEE1 of the cell cycle [1, 2]. (sagepub.com)
  • In general, NHEJ is the preferred pathway in the G1 phase of the cell cycle, while HR is favored in S and G2 phases. (cea.fr)
  • In conclusion, we have established a unique CCA stem-like cell model and identified the HMGA1-Aurora A signaling as an important pathway for CSC-CCA. (bvsalud.org)
  • Additionally, PIG3 mediates cancer cell death through the GPx3 pathway, and knocking down PIG3 or blocking the interaction between PIG3 and GPx3 would abolish the increase in ROS and apoptosis ( 5 ). (ijbs.com)
  • Our previous study demonstrated that PIG3 was induced in a dose-dependent manner by ionizing radiation (IR) ( 6 ). (ijbs.com)
  • In a short-term CA test using the fibroblast CHL cell line, the appearance of NS increased in a dose-dependent manner after exposure to six types of clastogens (mitomycin C, methyl methane sulfonate, 1-methyl-3-nitro-1-nitrosoguanidine, benzo[a]pyrene, cyclophosphamide monohydrate, and 9,10-dimethyl-2-benzanthracene), and a strong correlation was found between NS (%) and CAs (%) at each dosage. (biomedcentral.com)
  • RT-induced damage to cancer cells leads to different outcomes, such as survival, senescence, or death. (nature.com)
  • Aging kidney and CKD share many common characteristic features with increased cellular senescence, a conserved program characterized by an irreversible cell cycle arrest with altered transcriptome and secretome. (frontiersin.org)
  • Senescent cells elicit their fibrogenic actions primarily by secreting an assortment of inflammatory and profibrotic factors known as the senescence-associated secretory phenotype (SASP). (frontiersin.org)
  • Cellular senescence is characterized by an irreversible and permanent cell cycle arrest coupled with altered transcriptome and secretome. (frontiersin.org)
  • Unlike apoptosis, senescence is a state of which cells are still alive and metabolically active. (frontiersin.org)
  • Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. (proteopedia.org)
  • Floral transition is a critical event in the life cycle of a flowering plant as it determines its reproductive success. (biomedcentral.com)
  • We discovered an unprecedented burst of differential expression of cell cycle related genes at one particular point during transition to flowering. (biomedcentral.com)
  • Consequently, the transition to flowering is under strict genetic and environmental control [ 1 ], with floral initiation being induced by both external (photoperiod- and vernalization-dependent) and internal pathways (autonomous, age- and gibberellin-dependent) [ 2 - 4 ]. (biomedcentral.com)
  • Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. (wuxibiortus.com)
  • Histone synthesis is tightly coupled to DNA replication and peaks during the G1/S phase transition to account for doubling the genome. (biomedcentral.com)
  • 33 35 The pan caspase inhib itor Q VD OPh considerably increases differentiation of DCS handled 40AF cells. (cox2-inhibitors.com)
  • JNK1 shRNA expressing INS1 cells demonstrated elevated apoptosis and cleaved caspase 9 and 3 in comparison to nonsense shRNA expressing control INS1 cells when subjected to palmitate and high blood sugar associated with elevated CHOP appearance, ROS development and mRNA appearance. (cgp60474.com)
  • Finally, JNK3 shRNA expressing INS1 cells didn't induce apoptosis in comparison to nonsense shRNA expressing INS1 cells when subjected to palmitate and high blood sugar but showed elevated caspase 9 and 3 cleavage connected with elevated and mRNA appearance. (cgp60474.com)
  • Higher intracellular arsenic levels induced cell cycle arrest in the S-phase and G 2 -phase in SK-Mel-3 and SK-Mel-28 cells, respectively. (uky.edu)
  • His Apoptosis study integrates concerns from other disciplines, such as Intracellular pH, Intracellular and Chinese hamster ovary cell. (research.com)
  • Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
  • As main findings, 23 miRNAs were already identified as being involved in genetic regulation of PCa cell response to RT. (frontiersin.org)
  • In addition HIV-1 Vpr protein alters the cell-cycle regulation by hijacking centrosome functions. (biomedcentral.com)
  • However, the level in which these B[a]P regulated genes are expressed differs between Wt and Xpc-/- mice (p = 0.000000141), and were predominantly involved in the regulation of cell cycle, translation, chromatin structure and spermatogenesis, indicating a general stress response. (maastrichtuniversity.nl)
  • SHK induced apoptosis of ATLL cells accompanied by generation of reactive oxygen species (ROS), loss of mitochondrial membrane potential, and induction of endoplasmic reticulum (ER) stress. (bvsalud.org)
  • Whereas damage signals of cells activate p53 via CHK2 to repair damaged DNA, leading to cell cycle arrest. (jcpjournal.org)
  • Baicalein Induces G2/M Cell Cycle Arrest Associated with ROS Generation and CHK2 Activation in Highly Invasive Human Ovarian Cancer Cells. (tku.edu.tw)
  • Dysfunctional CHK2 impairs DNA damage-induced checkpoints, reduces apoptosis, and confers resistance to chemotherapeutic drugs and radiation therapy in ovarian cancer cells. (tku.edu.tw)
  • Eribulin inhibits the growth phase of microtubules without affecting the shortening phase and sequesters tubulin into nonproductive aggregates. (shu.edu)
  • Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
  • A slightly higher induction of average gene expression was observed at the G2-M checkpoint in Xpc-/- mice, but this did not reach statistical significance (P=0.086). (maastrichtuniversity.nl)
  • In some p53 mutants, induction of cell cycle arrest, but not apoptosis was found to be associated with a lack of induction of PIG3 expression ( 2 ). (ijbs.com)
  • β-peltatin initially arrested PAC cells at G2/M phase, followed by apoptosis induction. (biomedcentral.com)
  • An early apoptosis induction in HepG2 cells was observed by annexin V assay, which clearly exhibited significantly increased early and late apoptosis phases both at 24 and 48 h. (phcogj.com)
  • A significantly increasing pattern of hypodiploid phases of cells also been observed, which confirm the apoptosis induction again. (phcogj.com)
  • Obese and diabetic topics have raised plasma degrees of nonesterified essential fatty acids (NEFAs) and hyperglycemia, that are believed to trigger reduced insulin synthesis and impaired blood sugar responsiveness in pancreatic -cells, termed glucolipotoxicity [2] also, [3]. (cgp60474.com)
  • In combination with immune checkpoint blockade (ICB), epigenetic modification-targeted drugs are emerging as attractive cancer treatments. (biomedcentral.com)
  • These results provide a global view of the cell cycle and the method can be used to discover the dynamic coordination properties of functional components in other cellular processes. (biomedcentral.com)
  • We investigated its cellular localization and found it in both cytosolic and nuclear extracts derived from HeLa cells (Fig. 1 a). (biomedcentral.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • We found that Dusp4 loss alone is insufficient in mediating tumorigenesis, but alternatively converges with loss in Trp53 and MYC amplification to induce tumorigenesis primarily through chromosome 5 amplification, which specifically upregulates Dbf4 , a cell cycle gene that promotes cellular replication by mediating cell cycle checkpoint escape. (biomedcentral.com)
  • The TME consists of various cellular components, including immune cells, stromal cells, and extracellular matrix, along with soluble factors and signaling molecules. (biomedcentral.com)
  • Furthermore, H 2 O 2 -induced oxidative stress reduced JAM3 expression in trophoblasts and cell culture supernatants. (bioone.org)
  • One of the treatments applied in cancer is radiotherapy (RT), a therapeutic modality that uses ionizing radiation to induce damage in unwanted cells. (frontiersin.org)
  • The main goal of RT consists in delivering a precise dose of radiation in a target volume, such as tumor, promoting the tumor cells eradication with as minimal damage as possible in surrounding normal tissues ( 13 ). (frontiersin.org)
  • The DNA double-strand break (DSB) is considered to be the most severe type of DNA damage induced by ionizing radiation, and this form of DNA damage must be repaired immediately to prevent cell death. (ijbs.com)
  • In line with this, for HPV negative but not for HPV positive cell lines, treatment with roscovitine resulted in a pronounced enhancement of the radiation-induced G2 arrest as well as a significant increase in radiosensitivity. (oncotarget.com)
  • The aim of the present study was, therefore, to investigate the cytostatic and cytotoxic impact of the new iron chelator deferasirox (DFX) on human GBM cells in well-defined clinical situations represented by radiation therapy and mild-hypoxia. (biomedcentral.com)
  • DUSP4 alterations lead to hyperactivation of MAPK signaling in many cancers, including breast cancer, which often harbor mutations in cell cycle checkpoint genes, particularly in TP53. (biomedcentral.com)
  • The G2 M checkpoint is especially important in protecting normal cells from tumor formation driven by the accumulation of mutations. (hsp90-inhibitors.com)
  • The SDEG protein-protein discussion module analysis demonstrated that and could play a significant part in stress-induced immunosuppression. (moonphase2018.com)
  • A background is supplied by These findings for even more study on stress-induced immunosuppression. (moonphase2018.com)
  • In this review, we discuss the changes in irradiated cancer cells and immune cells in the TME under different RT regimens and describe existing and potential molecules that could be targeted to improve the therapeutic effects of RT. (nature.com)
  • ICP-MS analysis indicated that arsenite-resistant SK-Mel-28 cells did not accumulate less arsenic than arsenite-sensitive A375 cells, suggesting that resistance was not attributable to reduced arsenic accumulation but rather to intrinsic properties of resistant cell lines. (uky.edu)
  • Golgi fragmentation is a highly regulated process that allows division of the Golgi complex between the two daughter cells. (biologists.com)
  • RT can directly induce cancer cell death through various mechanisms, such as apoptosis, necrosis, and autophagy. (nature.com)
  • The mechanisms of radioresistance are still poorly understood, despite it has been suggested that miRNAs play an important role in cell signaling pathways. (frontiersin.org)
  • These results are consistent with previous studies and demonstrate that our methodology is effective for studying cooperative mechanisms in the cell cycle. (biomedcentral.com)
  • Maintaining genomic integrity is of utmost importance to eukaryotic cells, which have evolved sophisticated mechanisms to ensure speed, accuracy, and an adequate pool of nucleotide and replication factors as well as high-fidelity repair pathways to correct errors occurring during DNA replication. (bmj.com)
  • Although the S and M phases rarely are subjected to changes imposed by extracellular signals, the G1 and G2 phases are the primary periods when cells decide whether or not to move on to the next phase. (your-doctor.net)
  • His Programmed cell death research incorporates themes from Deoxyribonuclease II, Acetylation, Extracellular, Cell biology and Endonuclease. (research.com)
  • This indicates that high levels of p21 and Cdk2 can be expressed at different times in G2 M arrest and endoreduplication because endoreduplication occurs after G2 M arrest. (hsp90-inhibitors.com)
  • The present study has shown that an increase in p21 expression coincides with the onset of G2 M arrest at 24 h, but that endoreduplication may occur due to loss of p21 and a maximum level of Cdk2 at 48 h. (hsp90-inhibitors.com)
  • Change in localisation depends on the checkpoint kinases Tel1ATM and Mec1ATR and has a positive effect on spontaneous recombination. (cea.fr)
  • That SP600125 induces endoreduplication signals, promotes tubulin polymerization, a critical process in cell division, and induces delayed apoptosis in leukemia Aurora kinases cells. (hsp90-inhibitors.com)
  • In human BCa tissues, we observed that LAPTM5 was significantly induced at both mRNA and protein levels, which is consistent with our microarray result. (spandidos-publications.com)
  • PIG3 is highly homologous to NADPH oxidoreductase TED2 in plants and zeta-Crystalline in mammalian cells, and is considered as a proapoptosis marker. (ijbs.com)
  • Numerous studies have evaluated nuclear swelling (NS), including in cultured mammalian cells (e.g. (biomedcentral.com)
  • This illustration shows the proposed relative location of the 2 known cell cycle arrest points in cells with MTD (microtubule damage). (shu.edu)
  • In this study, we investigated by using microarray technology whether compromised DNA repair in Xpc-/- mice may lead to a transcriptional reaction of the testis to cope with increased levels of B[a]P induced DNA damage. (maastrichtuniversity.nl)
  • However, a compensatory feedback of increased mRNA expression of DNA-PKcs was formed in PIG3-depleted cells after a few passages or cell cycles of subculture, which led the recovery of the DNA-PKcs protein level and the consequent recovered efficiency of the DNA damage response. (ijbs.com)
  • This was the first evidence suggesting the involvement of PIG3 in the IR-induced DNA damage response. (ijbs.com)
  • J-H Lee et al extensively investigated the participation of PIG3 in DNA damage checkpoints after UV irradiation or after treatment using the radiomimetic drug neocarzinostatin (NCS) ( 8 ). (ijbs.com)
  • Outside S-phase a histone pool is maintained in case of unanticipated changes to histone demand, such as DNA damage [ 9 ]. (biomedcentral.com)
  • Strategies that increase replicative stress while lowering cell cycle checkpoint thresholds may allow unrepaired DNA damage to be inappropriately carried forward in replicating cells, leading to mitotic catastrophe and cell death. (bmj.com)
  • Furthermore, inflammatory mediators released by irradiated dying cells can attract and regulate immune cells in the tumor microenvironment (TME), further killing cancer cells. (nature.com)
  • One study reported an increased survival benefit with simultaneous RT compared with sequential administration, 8 while another study found no significant difference between the two strategies, 9 possibly because simultaneous RT plus immunotherapy and the administration of an ICI before RT may kill cancer cells as well a substantial number of immune cells, leading to poor systemic response and toxic side effects. (nature.com)
  • The abnormal cell growth is one of the critical characteristics of cancer for invasion or spreading out to other parts of the body. (jcpjournal.org)
  • Cancer cells can grow unusually via limitless number of cell division and can avoid apoptosis. (jcpjournal.org)
  • Cuc B, one of many extensively investigated cucurbitacins, lead to diverse phase cell cycle arrest in diverse cancer cells. (achrinhibitor.com)
  • The anti-proliferative effect of Cuc B on cancer cells has been reported everywhere. (achrinhibitor.com)
  • Here, we studied the correlations between the checkpoint with forkhead-associated and ring finger (CHFR) gene expression and responses to paclitaxel in endometrial cancer cells. (bmj.com)
  • We cultured 6 endometrial cancer cell lines exposed to paclitaxel, studied the cell cytotoxicity, cell cycle distribution, CHFR expression, and methylation status before and after a demethylation agent (5-aza) treatment. (bmj.com)
  • Our data suggest that CHFR suppression regulated by hypermethylation may sensitize endometrial cancer cells to paclitaxel, and CHFR may be a promising marker to predict the response of endometrial cancer to paclitaxel. (bmj.com)
  • His Cancer research study combines topics in areas such as Cancer, Cell cycle, Cisplatin, Immunology and Chronic lymphocytic leukemia. (research.com)
  • Alan Eastman interconnects Cancer, Cell cycle, Gemcitabine, Immunology and DNA repair in the investigation of issues within Cancer research. (research.com)
  • Using a genetically engineered mouse model, we generated mammary-specific Dusp4 -deleted primary epithelial cells to investigate the necessary conditions in which DUSP4 loss may drive breast cancer oncogenesis. (biomedcentral.com)
  • However, cancer cells exploit these checkpoints to evade immune surveillance and suppress antitumor immune responses. (biomedcentral.com)
  • Deferiprone, a bidentate iron chelator, has been shown to have antiproliferative and cytotoxicity activities in neuroblastoma cell lines [ 7 ]. (biomedcentral.com)
  • The lack of arsenite-induced mitotic arrest in resistant cell lines was associated with a weakened spindle checkpoint resulting from reduced expression of spindle checkpoint protein BUBR1. (uky.edu)
  • These data suggest that arsenite has potential for treatment of solid tumors but a functional spindle checkpoint is a prerequisite for a positive response to its clinical application. (uky.edu)
  • cleaved fragment of HPK1, amongst parental one,25D sensitive HL60 cells as well as the 40AF cells with acquired resistance to 1,25D, is shown in Figure 5C. (cox2-inhibitors.com)
  • KG 1a cells with innate resistance to 1,25D also express HPK1 C fragments, that are diminished by treatment method DCS. (cox2-inhibitors.com)
  • Vitamin D resistance of KG 1a, AML M1 type cells,39 may also be attenuated by treatment with DCS, and, as in adaptively resistant 40AF cells, this is certainly associated with all the disappearance of your cleaved fragment and concurrent improve inside the level of your FL HPK1. (cox2-inhibitors.com)
  • In addition, cells with CHFR demethylation resulted in G2/M phase arrest that induced to paclitaxel resistance. (bmj.com)
  • Objective This open-label Phase Ib study (NCT02341456) investigated the safety, pharmacokinetics, and clinical activity of adavosertib in combination with carboplatin alone or paclitaxel plus carboplatin in Asian patients with advanced solid tumors and defined the recommended Phase II dose. (sagepub.com)
  • administered in combination with cisplatin, carboplatin, or gemcitabine in patients with advanced solid tumors in a * Yung-Jue Bang [email protected] Phase I study (NCT00648648, PN001) [6]. (sagepub.com)
  • Thus, determining how cells cope with replicative stress can help our understanding of oncogenesis and lead to the identification of new antitumor treatment targets. (oncotarget.com)
  • JNK2 shRNA expressing INS1 cells didn't influence palmitate and high blood sugar induced apoptosis or ER tension markers, but increased appearance in comparison to non-sense shRNA expressing INS1 cells mRNA. (cgp60474.com)
  • SARS-CoV-2 vaccines, composed of mRNA-containing PEGylated lipid nanoparticles, have been widely administered as intramuscular (i.m.) injections, so it is important to determine if PEGylated formulations can induce anti-PEG antibodies. (matci.jp)
  • Gene Ontology (GO) enrichment analysis of these genes identified an overrepresentation of genes related to the cell cycle. (biomedcentral.com)
  • The p53-inducible gene 3 (PIG3) was originally indentified by Polyak et al in the analysis of p53-induced genes related to the onset of apoptosis ( 1 ). (ijbs.com)
  • Conclusions Adavosertib 175 mg bid for 2.5 days was chosen as the recommended Phase II dose in combination with pacli- taxel and carboplatin in Asian patients. (sagepub.com)
  • Dysregulation of various cells in the tumor microenvironment (TME) causes immunosuppressive functions and aggressive tumor growth. (biomedcentral.com)
  • During the last decade, the non-random spatial arrangement of the genome into the nucleus of eukaryotic cells, as emerged as a key regulator of genome functions and notably of the propagation of a stable genome. (cea.fr)
  • We have demonstrated that, in certain conditions, an intravenous (i.v.) injection of PEGylated liposomes (PEG-Lip) induced anti-PEG IgM antibodies, which led to rapid clearance of second doses in mice. (matci.jp)
  • The risk is increased 1000-fold for squamous cell carcinoma, basal cell carcinoma, malignant melanoma, and fibrosarcoma and is increased 10-fold to 20-fold for other tumors. (medscape.com)
  • This theory holds that tumors arise from 1 of 2 undifferentiated stem cells: the excretory duct reserve cell or the intercalated duct reserve cell. (medscape.com)
  • Animal study confirmed that β-peltatin significantly suppressed the growth of subcutaneously-implanted BxPC-3 cell xenografts. (biomedcentral.com)
  • ABSTRACT: BACKGROUND: Benzo[a]pyrene (B[a]P) exposure induces DNA adducts at all stages of spermatogenesis and in testis, and removal of these lesions is less efficient in nucleotide excision repair deficient Xpc-/- mice than in wild type mice. (maastrichtuniversity.nl)
  • Our results demonstrate that Pulsatillae chinensis and particularly its bioactive ingredient β-peltatin suppress PAC by triggering cell cycle arrest at G2/M phase and apoptosis. (biomedcentral.com)
  • They localize in close proximity to the nucleus for the duration of interphase and play major roles in numerous cell functions. (biomedcentral.com)