Induces apoptotic cell. Medical search. Frequent questions

Apoptosis is a distinct form of programmed cell death in which cells activate an intrinsic suicide program to self-destruct. (elifesciences.org)
Microglial cells are also associated with a novel subtype of pro-inflammatory programmed cell death known as pyroptosis. (aging-us.com)
It is also essential for development of nerval system by programmed cell death. (wikipedia.org)
cDNA hydridization array analysis with two differing Mn concentrations and time points, identified no noteworthy mRNA inductions of genes associated with programmed cell death. (cdc.gov)
There is increasing evidence that free fatty acids have the ability to trigger cell apoptosis and it has been shown that exposure of endothelial cells to E-LDL results in programmed cell death. (europa.eu)

In conclusion, the pro-apoptotic Bcl-2 family members (Bid and Bax) are the key mediators in relaying the cell death signaling initiated by Andro from caspase 8 to mitochondria and then to downstream effector caspases, and eventually leading to apoptotic cell death. (elsevierpure.com)
Other biological effects not leading to apoptotic cell death have also been described. (biolegend.com)

Cisplatin-induced cytotoxicity appears to be multifactorial and involves inflammation, oxidative stress as well as apoptosis. (uah.es)
Cytotoxicity and inflammation-associated toxic responses have been observed to be induced by bacterial lipopolysaccharides (LPS) in vitro and in vivo respectively. (uaeu.ac.ae)
Our results suggest that the altered redox metabolism, oxidative stress and mitochondrial function in HepG2 cells play a critical role in LPS/aspirin-induced cytotoxicity. (uaeu.ac.ae)
Manganese -induced cytotoxicity in dopamine-producing cells. (cdc.gov)
In conclusion, DA content was not responsible for the enhanced sensitivity of CATH.a cells to Mn toxicity, but oxidative stress was implicated as a probable mechanism of cytotoxicity. (cdc.gov)
However, potentially erroneous characterization of cytotoxicity and cell proliferation can arise if verification of baseline interaction of test article with method employed is neglected. (cdc.gov)
Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)

Eiger is produced in apoptotic cells and, through activation of the c-Jun N-terminal kinase (JNK) pathway, is able to propagate the initial apoptotic stimulus. (elifesciences.org)
found that the JNK pathway activated apoptosis in anterior cells. (elifesciences.org)
After Fas stimulation, Daxx is activated and plays its role of pro-apoptotic protein in activating the c-JUN-N-Terminal Kinase (JNK) pathway. (wikipedia.org)
This pathway normally regulates stress-induced cell death. (wikipedia.org)
The real apoptotic process starts after activating this pathway. (wikipedia.org)
When the cell is treated with TGF-β, HIPK2, a nuclear kinase, phosphorylates Daxx and the activated Daxx in turn activates the JNK pathway (see "The Daxx Pathway" figure). (wikipedia.org)
In this study, Andro induced apoptosis in human cancer cells via activation of caspase 8 in the extrinsic death receptor pathway and subsequently with the participation of mitochondria. (elsevierpure.com)
We have recently shown that the cyclo-oxygenase-2 (COX2)/intracellular prostaglandin E2 (iPGE2)/EP receptor pathway mediates the apoptotic effect of cisplatin on human proximal tubular HK-2 cells. (uah.es)
Inhibition of the COX-2/iPGE2/EP receptor pathway in these cells prevented the effects of ABs without interfering with their internalization. (uah.es)
ABs released by cells undergoing apoptosis upon treatment with ABs) did not trigger apoptosis in naïve HK-2 cells, and stimulated cell proliferation through the COX-2/iPGE2/EP receptor pathway. (uah.es)
Since iPGE2 also mediates both cisplatin-induced HK-2 cell apoptosis, intervention in the COX-2/iPGE2/EP receptor pathway might provide us with new therapeutic avenues in patients with cisplatin-induced acute kidney injury. (uah.es)
In both cases, an increased intracellular calcium ([Ca +2 ] i ) caused by antibody triggered the apoptotic pathway, and in patients, it can lead to degeneration of photoreceptors in the retina [ 6 - 9 ]. (biomedcentral.com)
Acts upstream of or within intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator and tumor necrosis factor-mediated signaling pathway. (nih.gov)
Interestingly, pro-apoptotic signaling from the PERK UPR pathway was also not induced. (nih.gov)
The PKCδ/p53 apoptotic signaling pathway was the pharmacological target of baicalin. (medscimonit.com)
Anticancer activity of adenine in colon cancer cells is attributable to the activation of apoptotic signaling and in turn the AMPK/mTOR pathway. (hindawi.com)
Here we show that chloroquine, a drug that activates the stress-responsive Atm-p53 tumor-suppressor pathway, preferentially enhances the death of Myc oncogene-overexpressing primary mouse B cells and mouse embryonic fibroblasts (MEFs) and impairs Myc-induced lymphomagenesis in a transgenic mouse model of human Burkitt lymphoma. (jci.org)
The results demonstrate that RINm5F beta-cells express a pertussis toxin sensitive pathway that is anti-apoptotic. (fluoridealert.org)
The activity of this pathway is most evident in cells exposed to pro-apoptotic stimuli where the effects of pertussis toxin can be blocked by inhibitors of tyrosine kinase enzymes. (fluoridealert.org)

Apoptotic cells can produce signals to instruct cells in their local environment, including ones that stimulate engulfment and proliferation. (elifesciences.org)
Resveratrol inhibits U251 proliferation, as measured by MTS assay [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt], and induces G0/G1 growth arrest, as determined by flow cytometry. (lacienciadelvino.com)
Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. (wikipedia.org)
Monitoring of cell proliferation in a time-dependent manner was performed with the xCELLigence RTCA DP system. (excli.de)
Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
This activation is necessary for both cell proliferation as well as glucose uptake and use. (hindawi.com)
TGF-β regulates a variety of different cellular developmental processes including growth, differentiation, proliferation, and cell death. (wikipedia.org)
Apoptosis and cell proliferation in proximal tubular cells exposed to apoptotic bodies. (uah.es)
The findings reveal that SOX12 knockdown could be a new target for reducing AML cells proliferation through antisense therapy approach. (ijmcmed.org)
Understanding the function of lectin-glycan recognition systems in cell death will facilitate the implementation of novel therapeutic strategies aimed at controlling unbalanced cell proliferation and survival in several pathologic conditions. (nature.com)
Cancer, characterized by uncontrolled cell proliferation, is treated by chemotherapy and radiotherapy to induce apoptosis and kill cancerous cells. (news-medical.net)
For apoptotic inducing activity, human osteogenic sarcoma (HOS) cell proliferation, chromosomal DNA degradation and changes in apoptosis-related protein levels were measured. (nutrimedical.com)
Ellagic acid significantly reduced HOS cell proliferation, and induced apoptosis evidenced by chromosomal DNA degradation and apoptotic body appearance. (nutrimedical.com)
All the compounds produced a significant reduction in HCT-116 cell line proliferation, except compounds 2 and 6. (mendeley.com)
However, the mechanism by which adenine inhibits the proliferation of cancer cells is sketchy. (hindawi.com)
In this study, we investigated the effects of adenine against the proliferation of colon cancer cells. (hindawi.com)

These results suggest that multiple signaling pathways may underlie the apoptotic death of U251 glioma induced by resveratrol, which warrants further exploration as an anticancer agent in human glioma. (lacienciadelvino.com)
Lipids, amino acids, and nucleotides necessary for the biosynthesis of the daughter cells are mostly provided by intermediate metabolites of these pathways. (hindawi.com)
Rhamnaceae) on cisplatin-induced damage to kidney epithelial LLC-PK1 cells via mitogen-activated protein kinase (MAPK) and apoptosis pathways. (mdpi.com)
10 Galectins can function inside the cells by modulating signaling pathways, 11 although they also act extracellularly by establishing multivalent interactions with cell surface glycans and delivering signals that lead to disruption of cellular homeostasis. (nature.com)
The expression of caspase-3 and caspase-9 genes increased in HCT-116 cell lines after 48 h of treatment, suggesting cell death by the apoptotic pathways. (mendeley.com)
One family of cell surface pattern recognition receptors (PRRs) named as Toll-like receptors TLRs recognize PAMPs through an extracellular domain and initiate inflammatory signaling pathways through an intracellular domain. (frontiersin.org)
However, when both apoptotic and autophagic pathways were blocked simultaneously, chloroquine-induced killing of Myc-overexpressing cells was blunted. (jci.org)
Yen ML, Su JL, Chien CL , Tseng KW, Yang CY, Chen WF, Chang CC, and Kuo ML. Diosgenin induces HIF-1 activation and angiogenesis through estrogen receptor-related PI3K/Akt and p38 MAPK pathways in osteoblasts. (ntu.edu.tw)
May participate in the GZMB apoptotic pathways. (lu.se)

We obtained 18 hybridoma clones recognizing protein targets in apoptotic HUH7 cells, and clone 6D5 was chosen for characterization studies because of its strong reactivity in cell-ELISA assay. (deu.edu.tr)
Consistently, knockdown of Bid protein using small interfering RNA (siRNA) technique suppressed Andro-induced Bax conformational change and apoptosis. (elsevierpure.com)
Western blotting showed a reduction in proapoptotic protein Bax in the cytoplasm and mitochondria of resveratrol-treated cells. (biomedcentral.com)
Here, high mobility group box-1 (HMGB-1) protein was explored as a potential mediator of stress-induced microglial priming and whether HMGB-1 does so via the nucleotide-binding domain, leucine-rich repeat, pyrin domain containing protein 3 (NLRP3) inflammasome. (jneurosci.org)
Receptor-interacting protein kinase 1 (RIPK1) is a key component of the tumor necrosis factor (TNF) receptor signaling complex that regulates both pro- and anti-apoptotic signaling. (mdpi.com)
In this study, we found that RING finger protein 4 (RNF4), a RING finger E3 ubiquitin ligase, is required for the RIPK1 autophosphorylation and subsequent cell death. (mdpi.com)
West Nile virus capsid (WNV-cp)-DJY protein expression induces apoptosis. (cdc.gov)
The Novel ALG-2 Target Protein CDIP1 Promotes Cell Death by Interacting with ESCRT-I and VAPA/B. Inukai R, et al . (nih.gov)
Rhodopsin protein loss occurred as soon as photoreceptors developed, preceding photoreceptor cell death. (nih.gov)
Cells were also treated with PKC activator PEP005 or specific siRNA against protein kinase Cδ (PKCδ). (medscimonit.com)
The data showed that adenine activated AMP-activated protein kinase (AMPK) signaling contributing to autophagic cell death through mTOR in both colon cancer cell lines. (hindawi.com)
Similarly, cleaved caspase 3 protein levels and numbers of TUNEL-positive cells were increased in CIN mice, but suppressed in CIN mice injected with SF-MSCs compared with S-MSCs. (asn-online.org)
In vitro experiments showed that the increase in cleaved caspase 3 protein level induced by radiation of HEK293 cells was more strongly suppressed by conditioned medium from SF-MSCs compared with that from S-MSCs. (asn-online.org)
Interestingly, chloroquine-induced cell death was not dependent on caspase-mediated apoptosis, as neither overexpression of the antiapoptotic protein Bcl-2 nor deletion of the proapoptotic Bax and Bak affected chloroquine-induced MEF death. (jci.org)
Phosphorylation of the pro-apoptotic protein BIK: mapping of phosphorylation sites and effect on apoptosis. (wikigenes.org)
the expression levels of protein associated with apoptosis and endoplasmic reticulum stress (ERS) of SKO-007 cells in various groups were detected by Western blotting method. (researchgate.net)
Objective: To investigate the effects of prion protein 106-126 peptide on inducing apoptosis in differentiated PC12 cells. (researchgate.net)
Methods: After differentiated by nerve growth factor (NGF), the PC12 cells were infected by prion protein 106-126 peptide. (researchgate.net)
Conclusion: Prion protein 106-126 peptide can induce apoptosis in differentiated PC12 cells and present cellular toxicity definitely. (researchgate.net)
Overexpression of neuronal intermediate filament protein internexin in PC 12 cells. (ntu.edu.tw)
Magnolol induces the distribvutional changes of p160 and adipose differentiation-related protein in adrenal cells. (ntu.edu.tw)
Subcellular fractionation and Western blot disclose that stimulation with LL-37 (8 μM) for 2 h triggers release of the mitochondrial protein apoptosis-inducing factor (AIF) to the cytosol, whereas LL-37 causes no release of cytochrome C oxidase subunit IV of the inner mitochondrial membrane, suggesting that LL-37 affects mitochondrial membrane. (lu.se)
Flow cytometric estimation of the apoptotic marker CD95 in peripheral neutrophils, lymphocytes and monocytes was done for 18 infants with non-oedematous protein energy malnutrition (PEM) and 12 oedematous ones, on hospital admission and after supervised nutritional rehabilitation, and compared with 12 matched controls. (who.int)
We specifically study Smac mimetics, small molecules mimicking the effect of the pro-apoptotic protein Smac. (lu.se)

Resveratrol induces activation of caspase-3 and increases the cleavage of the downstream caspase substrate, poly(ADP-ribose) polymerase. (lacienciadelvino.com)
Resveratrol-induced DNA fragmentation can be completely blocked by either a general caspase inhibitor (Z-VAD-FMK) or a selective caspase-3 inhibitor (Z-DEVD-FMK), but not by a selective caspase-1 inhibitor. (lacienciadelvino.com)
Resveratrol induces cytochrome c release from mitochondria to the cytoplasm and activation of caspase-9. (lacienciadelvino.com)
Stimulation of caspase 3 and the presence of DNA ladder suggested downstream apoptotic signalling were initiated. (um.edu.my)
This compound was tested in vitro on epimastigotes and trypomastigotes of T. cruzi for intracellular esterase activity, plasma membrane integrity, phosphatidylserine exposure, ROS production , mitochondrial membrane potential , caspase -like activity, DNA integrity, cell cycle and autophagy . (bvsalud.org)
Thus, the immunosuppressive effect is not essential for the cytoprotective properties of IPLs, and IPLs have multiple beneficial properties such as the anti-apoptotic effect, GSH-activating effect, and NTF-activating effect, although the anti-apoptotic effect of NI-IPLs is independent of the regulation of apoptotic activators such as caspase-3. (elsevierpure.com)
Resveratrol-pretreated cells also showed a significant decrease in intracellular calcium and an inhibition of caspase-3 activity as compared to the untreated cells. (biomedcentral.com)
In vitro protection of retinal cells from apoptosis by resveratrol occurred through multiple early molecular events, such as reduction of intracellular calcium levels, down-regulation of Bax, up-regulation of Sirt1 and Ku70 activities, and inhibition of caspase-3 activity. (biomedcentral.com)
In our recent study on antibody-induced apoptosis in retinal cells, the upregulated Bax translocated to mitochondria and triggered mitochondria-mediated caspase-3-mediated apoptosis and ultimately caused retinal cell death [ 6 , 9 ]. (biomedcentral.com)
Li HL, Chen DD, Li XH, Zhang HW, Lü JH, Ren XD, Wang CC. JTE-522-induced apoptosis in human gastric adenocarinoma cell line AGS cells by caspase activation accompanying cytochrome C release, membrane translocation of Bax and loss of mitochondrial membrane potential. (wjgnet.com)
However, apoptosis can be blocked by caspase inhibitors resulting in a "ballooning cell membrane", typical of necrosis. (news-medical.net)
Bax expression was induced and caspase-3 was activated by ellagic acid treatment. (nutrimedical.com)
CONCLUSION: Ellagic acid exhibited both antioxidant activity in V79-4 cells and apoptosis-inducing activity in HOS cells through the up-regulation of Bax and activation of caspase-3. (nutrimedical.com)
The caspase-3 and caspase-9 expressions and DAPI (4′, 6-diamidino-2-phenylindole) staining assays were used to evaluate the apoptotic activity. (mendeley.com)
Caspase-1 can also induce a non-apoptotic form of cell death named pyroptosis. (frontiersin.org)
Thus chloroquine induces lysosomal stress and provokes a p53-dependent cell death that does not require caspase-mediated apoptosis. (jci.org)
DNA fragmentation, caspase-3 activation, and apoptosis-related gene expression were studied in CATH.a cells. (cdc.gov)
Conclusion: BZM can greatly enhance the efficacy of LDM against multiple myeloma by increasing the levels of cleaved caspase-3 and PARP, and remarkably increase the apoptosis induced by LDM through further activation of ERS. (researchgate.net)
Yang KT, Pan SF, Chien CL , Hsu SM, Tseng YZ, Wang SM, and Wu ML. Mitochondrial Na+ overload is caused by oxidative stress and leads to activation of the caspase 3- dependent apoptotic machinery. (ntu.edu.tw)
Subsequently, caspase 8 is released from DISC and induces activation of caspase 3 and 7, proteolysis of cellular components, and apoptosis. (biolegend.com)
The possibility that cytotoxic effects were due to apoptosis was indicated by significant time- and dose-dependent induction of caspase-3/7 activity in both HepG2 cells and human hepatocytes. (cdc.gov)
Fipronil mediated activation of caspase-3/7 in concurrence with compromised ATP production and viability are attributed to apoptotic cell death. (cdc.gov)
In addition, my experiments showed that the active form of p38 associates with caspase 8 and caspase 3, which is necessary for p38-induced phosphorylation of serine-362 and serine-150 on these caspases. (lu.se)
These biochemical modifications impair the activities, and possibly also the stability, of caspase 8 and 3 and thereby weaken the capacity of these enzymes to induce apoptosis. (lu.se)
Consequently, PP2A can increase the activity of caspase 3 by dual mechanisms and thereby promote the apoptotic response in human neutrophils. (lu.se)

Our results suggest that astaxanthin has a neuroprotective effect against RGC death induced by glutamate stress, oxidative stress, and hypoxia, which induce apoptotic and necrotic cell death. (molvis.org)
Lymphadenitis results from apoptotic cell death induced by cytotoxic T lymphocytes. (medscape.com)
Effects of tyrosine kinase inhibitors on cell death induced by sodium fluoride and pertussis toxin in the pancreatic beta-cell line, RINm5F. (fluoridealert.org)
2. Exposure of RINm5F cells to low concentrations of genistein or tyrphostin A25 resulted in significant inhibition of cell death induced by 5 mM NaF. (fluoridealert.org)
3. The increase in cell death induced by 100 microM genistein was markedly inhibited by ciprofloxacin, a drug which binds to topoisomerase II. (fluoridealert.org)
4. Pertussis toxin markedly enhanced the extent of RINm5F cell death induced by NaF and this effect was completely prevented by 25 microM genistein. (fluoridealert.org)
ED 50 = 10 - 50 ng/ml, corresponding to a specific activity of 0.02 - 0.1 x 10 6 units/mg, as determined by inhibition of apoptotic cell death induced by FASL on Jurkat cells. (biolegend.com)

We hypothesise that delayed or altered clearance of apoptotic cells after UV irradiation will lead to inflammation in the skin of SLE patients. (unboundmedicine.com)
However, the presence of inflammatory lesions in the vicinity of apoptotic cells, as observed both in UVB-induced and in LE skin lesions in SLE patients, suggests that these lesions result from an inflammatory clearance of apoptotic cells. (unboundmedicine.com)
Thus, in addition to its established role in the sensing and clearance of apoptotic cells, C1q may also regulate fatty acid-induced apoptosis. (europa.eu)

To clarify the molecular basis of the cytoprotective properties of immunophilin ligands (IPLs), the anti-apoptotic effects of IPLs were determined in human glioma U251 cells. (elsevierpure.com)
Adipose-derived SF-MSCs attenuate CIN by exerting anti-apoptotic effects, indicating that SF-MSCs may be a potential therapy for CIN. (asn-online.org)

found that TNF is produced by apoptotic cells in hair follicles, and that blocking TNF inhibits the death of other cells in the same cohort: this suggests that a common mechanism could regulate the communal death of cells in flies and mammals. (elifesciences.org)

In the present study, we have further demonstrated that HepG2 cells treated with LPS alone or in combination with aspirin induces subcellular toxic responses which are accompanied by increase in reactive oxygen species (ROS) production, oxidative stress, mitochondrial respiratory dysfunction and apoptosis. (uaeu.ac.ae)
Permanent elevation of hearing threshold and loss of hair cells (HCs) are the main feature of NIHL [ 20 ], which are mostly due to reactive oxygen species (ROS) accumulation including free radicals superoxide (O 2 ·− ), hydroxyl radical and singlet oxygen. (biomedcentral.com)

Maprotiline restores ER homeostasis and rescues neurodegeneration via Histamine Receptor H1 inhibition in retinal ganglion cells. (stanford.edu)
Inhibition of cell viability was assessed with focus on apoptosis transcriptionally and translationally. (hindawi.com)
Inhibition of cytotoxic effect of FASL on Jurkat cells in the presence of recombinant human FAS. (biolegend.com)

The purpose of this study was to investigate whether astaxanthin confers a neuroprotective effect against glutamate stress, oxidative stress, and hypoxia-induced apoptotic or necrotic cell death in primary cultures of rat retinal ganglion cells (RGCs). (molvis.org)
In TEM examinations of HEC-1A cells, perifosine appeared to lead autophagic cell death, whereas vitamin D caused paraptosis-like cell death and combination of perifosine+vitamin D caused apoptotic and non-apoptotic (paraptotic, autophagic and necrotic) cell death. (excli.de)
Now, necrosis is known to have programmed aspects, with "necroptosis" describing regulated necrotic cell death. (news-medical.net)
They found that TNF induced both apoptotic and necrotic cell death. (news-medical.net)
This result sparked further interest in how necrotic cell death occurs. (news-medical.net)
Studies show that necrotic death is induced by a ligand binding to a receptor, such as when apoptosis is initiated. (news-medical.net)
Necroptosis can therefore start in an apoptosis-like manner, but result in necrotic-like cell death. (news-medical.net)
The induced necrotic cell death by virial agents is important for virus-induced inflammation, and blockage of necroptosis is a primary concern for controlling certain viral diseases. (news-medical.net)

Human glioma U251 cells were used to understand the molecular mechanisms by which resveratrol acts as an anticancer agent, since glioma is a particularly difficult cancer to treat and eradicate. (lacienciadelvino.com)
Therefore, it is considered that the combination of both drugs in the treatment of endometrial cancer might be an alternative and effective treatment option through activating the apoptotic and non-apoptotic cell death mechanisms in cancer cells. (excli.de)
In this paper, we describe the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, underlying the connection between these two processes. (hindawi.com)
These results suggest that ABs, through iPGE2-dependent mechanisms, might have a relevant role in the natural history of cisplatin-induced acute kidney failure because they contribute first to the propagation of the noxious effects of cisplatin to non-injured PTC and then to the promotion of the proliferative tubular response required for proximal tubule repair. (uah.es)
Two decades later, pioneering studies suggested that lectin-like molecules constitutively expressed on the surface of macrophages can selectively recognize changes on glycans decorating the surface of apoptotic thymocytes, 4 , 5 although these studies likewise did not provide substantial insight into the mechanisms by which lectin-glycan interactions regulate cell death. (nature.com)
Apoptosis and necrosis are characterized by different mechanisms leading to cell death. (news-medical.net)
In such cases, alternate mechanisms of cell death, such as necroptosis can be successfully induced. (news-medical.net)
We explored the possible mechanisms underlying the anticancer activity of adenine in colorectal cancer cells. (hindawi.com)
In the present study, tyrosine kinase inhibitors were used to investigate the mechanisms of action of NaF and pertussis toxin in the beta-cell line, RINm5F. (fluoridealert.org)
Our aims are to identify and understand mechanisms regulating breast cancer cell death and how inflammatory signaling can be induced and influence breast cancer progression. (lu.se)
In this dissertation thesis, I describe and elucidate intracellular signalling mechanisms that are involved in regulation of spontaneous and Fas-induced apoptosis in human neutrophils. (lu.se)
In this study, we investigated programmed death-ligand 1 (PDL-1) as a potential biomarker for CAF-induced chemoresistance and evaluated its role and the underlying mechanisms of chemoresistance in non-small cell lung cancer (NSCLC). (cdc.gov)

The number of live RGCs was then counted using a cell viability assay. (molvis.org)
Cell viability was determined by MTT assay. (ijmcmed.org)
Although one typically thinks of carbohydrates as associated with cell growth and viability, glycosylation also has an integral role in many processes leading to cell death. (nature.com)
Endometrium-derived epithelial cells (Ishikawa) showed no response to the natural compound by using a cell viability assay (MTT). (nutrimedical.com)
Cell viability was estimated by cell counting kit-8 and cell apoptosis by flow cytometry. (karger.com)
LPS effectively inhibited cell viability and LC3B I expression and enhanced LC3B II, Beclin-1 and atg5 expressions in MLE-12 cells. (karger.com)
In LPS-induced ARDS cell model, astragaloside IV up-regulated cell viability, SOD activity and ZO-1 and LC3B I expressions but down-regulated cell apoptosis, TNF-α, IL-6, LC3B II, Beclin-1 and atg5 expressions and LDH and MDA levels. (karger.com)
3-methyladenine promoted cell viability and ZO-1 expression, down-regulated Beclin-1 and atg5 expression, while Rapamycin (Rap) had an opposite effect. (karger.com)
Astragaloside IV suppressed cell viability and ZO-1 expression after the Rap treatment. (karger.com)
Astragaloside IV might suppress autophagy initiation directly or indirectly through suppressing the oxidative stress and inflammatory response, which further enhances the cell viability and tight junction and reduces apoptosis in LPS-stimulated pulmonary endothelial ARDS cell model, thus exerting its therapeutic function in ARDS. (karger.com)
The viability of the HEK-293 normal cells was found to be significantly higher than the viability of the cancerous cells at all of the tested concentrations, thus suggesting that all the compounds have better inhibitory activity on the cancer cell line. (mendeley.com)
Cell viability was measured using the MTT assay. (hindawi.com)
Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2 cells, in a dose-dependent manner. (hindawi.com)
Cell viability was determined by using the MTT assay and the morphological changes were observed. (researchgate.net)

Cell membrane morphology changes were examined by annexin V staining/flow cytometry by using HeLa cells transfected with pcWNV-Cp-DJY or control pcDNA3.1 plasmids (o). (cdc.gov)
Flow cytometry was used to detect cell apoptosis. (medscimonit.com)
Apoptotic was detected by morphological changes, DNA electrophoresis and flow cytometry. (researchgate.net)
Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis. (researchgate.net)

Huang Y , Tan F , Zhuo Y , Liu J , He J , Duan D , Lu M , Hu Z , . Hypoxia-preconditioned olfactory mucosa mesenchymal stem cells abolish cerebral ischemia/reperfusion-induced pyroptosis and apoptotic death of microglial cells by activating HIF-1α. (aging-us.com)
Results showed that OGD/R induced apoptosis and pyroptosis in microglial cells while hypoxia in OM-MSCs significantly attenuated these effects. (aging-us.com)
Taken together, these findings reveal that hypoxia-preconditioned OM-MSC inhibits pyroptotic and apoptotic death of microglial cell in response to cerebral ischemia/reperfusion insult by activating HIF-1α in vitro . (aging-us.com)
In this study, we investigate the influence of AST against glutamate- and hypoxia-induced apoptosis, as well as oxidative stress-induced necrosis, in primary rat RGC cultures. (molvis.org)
In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)
Cultured human arterial endothelial cells (HAECs) were exposed to hypoxia/deoxygenation (H/R). Cells were also treated with baicalin at serially diluted concentrations. (medscimonit.com)

Moreover, cellular proteins recognized by the antibody displayed a late upregulation in UVC- induced apoptotic cells. (deu.edu.tr)
Besides, downregulation of SOX12 in both cell lines could induce apoptosis, probably through upregulation of CASP3 and CASP9 . (ijmcmed.org)
Mechanistically, an upregulation of PDL-1 in chemotherapy-treated CAFs led to an increase in hepatocyte growth factor (HGF) secretion, which stimulates cancer progression, cell invasion, and stemness of lung cancer cells, while inhibiting apoptosis. (cdc.gov)

created 'undead' cells in which one of these proteins was turned on, but other downstream proteins (that are responsible for the cellular execution phase of apoptosis) had been turned off: these cells were undergoing apoptosis, but could not complete the process and die. (elifesciences.org)
The formation of necrosome, a multiprotein complex that stimulates necroptosis, is tightly regulated by proteins downstream of the receptor and can be the deciding factor in inducing necroptosis. (news-medical.net)
The active dimeric enzyme is then liberated from the disc and free to activate downstream apoptotic proteases. (lu.se)

Our in vivo studies demonstrated NC to be more bio-available than FC and showed a better tissue distribution profile without inducing any toxicity (100mg/kg bw) in mice. (bgu.ac.il)
The LPS/Aspirin induced toxicity was attenuated by pre-treatment of cells with N-acetyl cysteine (NAC). (uaeu.ac.ae)
In the present study, Mn toxicity was characterized in a human neuroblastoma (SK-N-SH) cell line and in a mouse catecholaminergic (CATH.a) cell line. (cdc.gov)

The existence of apoptotic bodies, cell membrane blebbing and chromatin condensation indicated the hallmark of apoptosis. (um.edu.my)
Macrophages were ingesting multiple apoptotic bodies. (unboundmedicine.com)
It has been associated with heterochromatin and PML-NBs (Promyelocytic Leukaemia nuclear bodies) and has been implicated in many nuclear processes including transcription and cell cycle regulation. (wikipedia.org)
Here, we studied the effects on HK-2 cells of apoptotic bodies (ABs) generated after treatment of HK-2 cells with cisplatin. (uah.es)

To address this problem, we encapsulated chelidonine in biodegradable poly(lactide-co-glycolide) (PLGA) polymers and evaluated nano-chelidonine's (NCs) anti-cancer efficacy vis-à-vis free chelidonine (FC) against HepG2 cells and also evaluated its bioavailability in mice. (bgu.ac.il)
NC exhibited rapid cellular uptake and stronger apoptotic effect (~46.6% reduced IC 50 value) than FC, blocking HepG2 cells at G2/M phase. (bgu.ac.il)
Our previous studies on aspirin treated HepG2 cells strongly suggest cell cycle arrest and induction of apoptosis associated with mitochondrial dysfunction. (uaeu.ac.ae)
Pre-treatment of HepG2 cells with NAC exhibited a selective protection in redox homeostasis and mitochondrial dysfunction. (uaeu.ac.ae)
Raza, H , John, A & Shafarin, J 2016, ' Potentiation of LPS-induced apoptotic cell death in human hepatoma HepG2 cells by aspirin via ROS and mitochondrial dysfunction: Protection by N-acetyl cysteine ', PLoS ONE , vol. 11, no. 7, e0159750. (uaeu.ac.ae)
HepG2 cells were incubated with troglitazone, its metabolites M-1 (sulfate), M-2 (gulucronide), M-3 (quinone), and other thiazolidinediones (pioglitazone and rosiglitazone). (nih.gov)
Cytotoxic effects of fipronil and fipronil sulfone were examined using the adenylate kinase and the trypan blue exclusion assays in HepG2 cells and human hepatocytes. (cdc.gov)
The results indicate both that HepG2 cells and primary human hepatocytes are sensitive to the cytotoxic effects of fipronil. (cdc.gov)
A significant time- and dose-dependent induction of adenylate kinase activity in HepG2 cells was noted from 0.1 to 12.5 µM fipronil followed by decreasing activities at 25 and 50 µM. (cdc.gov)

abstract = "Poor oral bioavailability of chelidonine, a bio-active ingredient of Chelidonium majus, showing anti-cancer potentials against cancer cells with multidrug resistance, makes its optimal use rather limited. (bgu.ac.il)

We previously showed that culturing mesenchymal stem cells (MSCs) in serum-free medium enhanced their anti-inflammatory effects. (asn-online.org)
Objective: To investigate the induction of tanshinone [1 A (Tan fl A) on the differentiation of human placenta-derived mesenchymal stem cells (hPDMSCs) into cardiomyocytes, and to provide an experimental basis for Tan IT A as a cardiomyocytc differentiation inducer. (researchgate.net)
Highly efficient cellular labeling of mesoporous nanoparticles in human mesenchymal stem cells: implication for stem cell tracking. (ntu.edu.tw)

These induce extracellular Daxx to translocalize into the cytoplasm following an association with ASK1 (Apoptosis signal-regulating kinase1). (wikipedia.org)
Glycans, either alone or complexed with glycan-binding proteins, can deliver intracellular signals or control extracellular processes that promote initiation, execution and resolution of cell death programs. (nature.com)
What is the precise role of intracellular and extracellular galectins in the control of cell death programs? (nature.com)

Our data show that resveratrol induces dose- and time-dependent death of U251 cells, as measured by lactate dehydrogenase release and internucleosomal DNA fragmentation assays. (lacienciadelvino.com)
DNA fragmentation in WNV capsid-expressing cell lines was examined by terminal desoxyriboxyl-desoxyriboxyl trasferase-mediated DVTP nick-end labeling (TUNEL) assay in HeLa (g), HEK 293 (i), and RD cells (k), and compared with DNA fragmentation from pcDNA3.1-transfected HeLa cells (m). (cdc.gov)
In 1988, Laster and colleagues exposed two cell lines to TNF to investigate whether TNF (tumor necrosis factor) targets underwent DNA fragmentation. (news-medical.net)
Troglitazone induced apoptotic cell death characterized by internucleosomal DNA fragmentation and nuclear condensation. (nih.gov)

These results may help in better understanding the pharmacological, toxicological and therapeutic properties of NSAIDs in cancer cells exposed to bacterial endotoxins. (uaeu.ac.ae)
Adenine is involved in a variety of cell biological processes and has been explored for pharmacological uses. (hindawi.com)

On the other hand, proteins involved in tumorigenesis are extensively modulated on exposure to apoptotic stimuli, including ultraviolet (UVC) irradiation. (deu.edu.tr)
Hence, we generated monoclonal antibodies by using UVC-irradiated apoptotic cells of an HCC cell line, HUH7, aiming to explore proteins differentially expressed in tumors and apoptosis. (deu.edu.tr)
We concluded that 6D5 target proteins are modulated in liver tumorigenesis and apoptotic processes. (deu.edu.tr)
Herein, we review the role of glycans and glycan-binding proteins as essential components of the cell death machinery during physiologic and pathologic settings. (nature.com)
At that time, glycobiology, which is the study of carbohydrates and their recognition by motif-specific carbohydrate-binding proteins or lectins, lagged far behind the studies that defined the structural and cellular biology of cell death. (nature.com)
Cell death by necroptosis involves membrane breakage, which leaks intracellular molecules such as heat shock proteins. (news-medical.net)

It affected intracellular esterase activity, produced important plasma membrane damage and induced phosphatidylserine exposure. (bvsalud.org)
Combined exposure to DEET (N,N-diethyl- m -toluamide) and permethrin-induced release of rat brain mitochondrial cytochrome c. (cdc.gov)
Neonatal exposure to a Type-I pyrethroid (bioallethrin) induces dose-response changes in brain muscarinic receptors and behaviour in neonatal and adult mice. (cdc.gov)
Nanotube exposure induces the nanotechnology industry is a multibillion dollar industry and is expected to reach a trillion dollars by 2015. (cdc.gov)

This occurs frequently in animal development: in human fetuses, for example, cells in the hand are directed to die to remove webbing between the fingers. (elifesciences.org)
Resveratrol-induced apoptotic death in human U251 glioma cells. (lacienciadelvino.com)
In this study, we aimed to investigate the anti-proliferative and apoptotic effects of varying concentrations of perifosine and vitamin D on the human endometrial cancer cell line (HEC-1A). (excli.de)
Plagioneurin B was isolated via conventional chromatography and tested for thorough mechanistic apoptosis activity on human ovarian cancer cells (CAOV-3). (um.edu.my)
Tanaka, KI , Asanuma, M & Ogawa, N 2004, ' Molecular basis of anti-apoptotic effect of immunophilin ligands on hydrogen peroxide-induced apoptosis in human glioma cells ', Neurochemical Research , vol. 29, no. 8, pp. 1529-1536. (elsevierpure.com)
In this study, SOX12 was blocked by antisense LNA GapmeRs (ALG) in human AML cell lines (KG1 and M07e). (ijmcmed.org)
Dabiri A, Sharifi M, Sarmadi A. Knockdown of SOX12 Expression Induced Apoptotic Factors is Associated with TWIST1 and CTNNB1 Expression in Human Acute Myeloid Leukemia Cells. (ijmcmed.org)
The human neuroblastoma cell line SH-SY5Y was transfected with Bax as a positive control (p), pcWNV-Cp (r), or control plasmid (t) and examined by TUNEL assay. (cdc.gov)
In the present study, the mechanism for the hepatic injury by troglitazone was investigated with human hepatoma cell lines. (nih.gov)
Chloroquine-induced cell death in primary MEFs and human colorectal cancer cells was dependent upon p53, but not upon the p53 modulators Atm or Arf. (jci.org)
Methods: The human multiple myeloma SKO-007 cells in logarithm growth phase were selected and randomly divided into control group, LDM group, BZM group, and BZM combined with LDM group. (researchgate.net)
The Apoptotic Process of Human Bladder Carcinoma T24 Cells Induced by Retinoid. (ntu.edu.tw)
FAS was initially purified and cloned from SKW6.4 cells and a cDNA library of human T cell lymphoma KT-3 cells. (biolegend.com)
In addition, FAS/FASL interaction increases the production of chemokines in NK-T cells, and FASL acts as a survival factor for human CD34 + cells and increases their colony formation. (biolegend.com)
Human FAS, amino acids (Arg17-Asn173) (Accession# NM_000043), was expressed in 293E cells. (biolegend.com)
The human antimicrobial peptide LL-37 permeabilizes the plasma membrane of host cells, but LL-37-induced direct effects on mitochondrial membrane permeability and function has not been reported. (lu.se)
Here, we demonstrate that LL-37 is rapidly (within 20 min) internalized by human osteoblast-like MG63 cells, and that the peptide co-localizes with MitoTracker arguing for accumulation in mitochondria. (lu.se)
The nanotube bundles are similar to the potential of nanotubes to induce genetic damage size of microtubules that form the mitotic spindle in normal lung cells, cultured primary and immor- and may be incorporated into the mitotic spindle talized human airway epithelial cells were apparatus. (cdc.gov)
The human neutrophil is the most abundant granulocyte and the major type of cell involved in an acute inflammatory response. (lu.se)

Cisplatin-induced LLC-PK1 cell death was most significantly reduced following treatment with 3-dehydroxyceanothetric acid 2-methyl ester (3DC2ME). (mdpi.com)

Considering the possible implication of endothelial cell apoptosis in the atherogenesis process, such a property would bear important biological implications. (europa.eu)

This compound also induced a cell cycle arrest in the G1 phase and the presence of autophagy vacuoles . (bvsalud.org)
Adenine has been suggested to induce cell cycle arrest in cancer cells, leading to cell death [ 9 , 10 ]. (hindawi.com)

These findings suggest that the cardioprotective potential of HupA is associated with its antioxidant, anti-apoptotic and anti-inflammatory properties in acute myocardial infarction in rats. (spandidos-publications.com)
Contrast-induced nephropathy (CIN) is a major clinical problem associated with acute kidney injury during hospitalization, however therapeutic methods for CIN have made little progress. (asn-online.org)
[ 1 , 2 ] SCARs include Stevens-Johnson syndrome (SJS), toxic epidermal necrolysis (TEN), drug reaction with eosinophilia and systemic symptoms (DRESS)/drug-induced hypersensitivity syndrome (DIHS or HSS) and acute generalized exanthematous pustulosis (AGEP). (medscape.com)

The anticancer activity of these compounds was evaluated in colon cancer, HCT-116, and noncancerous, HEK-293, cell lines using the MTT antiproliferative assays. (mendeley.com)
Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)

It induces the cancerous cell to commit suicide. (911weknow.com)

In contrast to normal cells, most cancer cells predominantly produce energy by a high rate of glycolysis followed by lactate fermentation, even in the presence of oxygen, a less efficient metabolism compared to a low rate of glycolysis followed by mitochondrial oxidation of pyruvate [ 2 ]. (hindawi.com)
Mammea type coumarins isolated from Calophyllum brasiliense induced apoptotic cell death of Trypanosoma cruzi through mitochondrial dysfunction, ROS production and cell cycle alterations. (bvsalud.org)
We conclude that LL-37 is rapidly internalized by MG63 cells and accumulates in mitochondria, and that the peptide triggers release of pro-apoptotic AIF and directly affects mitochondrial membrane structural properties. (lu.se)

Glycosylation of classical death receptors fine-tunes cell death programs. (nature.com)
The "death receptors", which typically cause cell death by apoptosis, can be activated by ligands, such as TNF. (news-medical.net)
Host cells express a range of receptors that act as microbial sensors. (frontiersin.org)
Sensing of the presence of microbes or their factors in the cytosol is mediated by NOD-like receptors (NLRs) and leads to signaling cascades that mediate the production of inflammatory cytokines, recruitment of phagocytic cells and control of the acquired immune response. (frontiersin.org)

It was observed that perifosine has increased the expression of BAX mRNA in HEC-1A cells in a dose-dependent manner. (excli.de)
While perifosine+vitamin D combinations increased P53 mRNA expression in HEC-1A cells we did not find any significant change in BCL2, BAX mRNA expression levels. (excli.de)
In a similar manner, CYP3A4 mRNA expression was also induced (10-30-fold), although at the higher doses induction returned to near control levels. (cdc.gov)

Host cells use several strategies to recognize specific pathogen-associated molecular patterns (PAMPs). (frontiersin.org)

We also observed the secretion of 6D5 ligands by some of the HCC cell lines. (deu.edu.tr)

In vivo clearance rate of apoptotic cells is comparable between SLE patients and controls. (unboundmedicine.com)
Importantly, apoVs administration effectively attenuated NIHL and reduced hair cell loss by resisting oxidative damage in vivo. (biomedcentral.com)
Hippocampal microglia were isolated 24 h later and stimulated with LPS ex vivo to probe for stress-induced sensitization of proinflammatory responses. (jneurosci.org)
Longitudinal In Vivo Ca 2+ Imaging Reveals Dynamics Activity Changes of Diseased Retinal Ganglion Cells at Single-Cell Level. (stanford.edu)
In vivo experiments were conducted using a co-implantation xenograft mouse model with live cell imaging and immunohistochemistry. (cdc.gov)

Is disturbed clearance of apoptotic keratinocytes responsible for UVB-induced inflammatory skin lesions in systemic lupus erythematosus? (unboundmedicine.com)
Inflammatory lesions in these patients were localised near accumulations of apoptotic keratinocytes similar as was seen in the majority of LE skin lesions. (unboundmedicine.com)

Further evaluation revealed that ellagic acid was a potent antiestrogen in MCF-7 breast cancer-derived cells, increasing, like the pure estrogen antagonist ICI182780, IGFBP-3 levels. (nutrimedical.com)
Single-cell transcriptome analysis of regenerating RGCs reveals potent glaucoma neural repair genes. (stanford.edu)

Priscilla DH and Prince PS: Cardioprotective effect of gallic acid on cardiac troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in Wistar rats. (spandidos-publications.com)
On the one hand, ROS induced the formation of lipid peroxidation products and led to hair cell apoptosis [ 23 ]. (biomedcentral.com)

These results suggest that troglitazone induces apoptotic hepatocyte death which it may be one of the factors of liver injury in humans. (nih.gov)

The binding of FASL to FAS induces conformational changes in FAS, leading to the recruitment of FADD and procaspase-8 and the assembly of the death-inducing signaling complex (DISC). (biolegend.com)
The interaction of FAS/FASL in immature dendritic cells (DCs) induces functional maturation. (biolegend.com)

Microglial cells are the first line immune cells that initiate inflammatory responses following cerebral ischemia/reperfusion(I/R) injury. (aging-us.com)
Research has been directed at developing treatments that modulate inflammatory responses and protect against cell death caused by cerebral I/R. Key among such treatments include mesenchymal stem cell (MSC) therapy. (aging-us.com)
Adenine induced significant apoptosis in HT29 cells, whereas Caco-2 cells exhibited less apoptotic responses. (hindawi.com)
1987. Biochemical responses to induced intoxication with insecticides: 1. (cdc.gov)

Apoptotic features such as chromatin condensation and nuclear shrinkage were also induced by the compounds. (mendeley.com)

25 microM) were cytotoxic in the absence of NaF but, paradoxically, the combination of genistein and NaF induced less cell death than when each agent was used alone. (fluoridealert.org)
However, fipronil sulfone induced cytotoxic effects at lower doses. (cdc.gov)

We identified Eiger, the Drosophila tumor necrosis factor (TNF) homolog, as the signal responsible for apoptosis-induced apoptosis (AiA). (elifesciences.org)
and all cases showed typical morphology of Kikuchi disease, with necrosis, karyorrhectic debris, and the presence of the typical cell types, namely crescentic histiocytes and plasmacytoid monocytes. (medscape.com)

Five out of 15 patients developed a dermal infiltrate that was associated with increased epidermal influx of T cells and macrophages but not with numbers of apoptotic cells or epidermal deposition of immunoglobulins. (unboundmedicine.com)

In normal cells, glucose participates in cellular energy production through glycolysis as well as through its complete catabolism via the tricarboxylic acid (TCA) cycle and oxidative phosphorylation (OXPHOS). (hindawi.com)
The pattern of the Ku70 cellular localization also overlapped with the Bax cellular localization in treated and untreated cells. (biomedcentral.com)

BACKGROUND: Antioxidant, antiproliferative and apoptosis inducing activities of a natural polyphenolic compound, ellagic acid, were studied. (nutrimedical.com)
Compound 48/80-induced degranulation of GnRH-like immunoreactive mast cells in the brain and mesentry of the gerbil. (ntu.edu.tw)

Plagioneurin B was also seen to arrest CAOV-3 cells cycle at the G2/M phase. (um.edu.my)

These findings uncovered the role of apoVs in preventing NIHL and resisting oxidative damage, indicating that apoVs is a promising way for inner ear delivery and a prospective cell-free therapy for NIHL. (biomedcentral.com)

It has been reported that RNF4 negatively regulates TNF-α-induced activation of the nuclear factor-κB (NF-κB) through downregulation of transforming growth factor β-activated kinase 1 (TAK1) activity, indicating the possibility that RNF4-mediated TAK1 suppression results in enhanced sensitivity to cell death. (mdpi.com)
Nuclear condensation was observed in HeLa cells transfected with pcWNV-Cp-DJY (a), a positive control pBax (b), or a negative control, pcDNA3.1 (c), under a 4,6-diamidino-2-phenylidole (DAPI) filter (magnification: 200X). (cdc.gov)
Nuclear staining in HeLa (h), HEK 293 (j), and RD cells (l) were observed by using a DAPI filter and compared with control HeLa cells (n) (magnification for g through n: 400X). (cdc.gov)
To visualize nuclear staining, cells transfected with pBax, pcWNV-Cp-DJY (q and s, respectively) or pcDNA3.1 (u) were stained with DAPI and observed using appropriate filters (magnification: 400X). (cdc.gov)
Apoptosis is associated with nuclear disintegration, followed by removal of the cell by macrophages. (news-medical.net)
Nuclear efflux of heterogeneous nuclear ribonucleoprotein C1/C2 in apoptotic cells: a novel nuclear export dependent on Rho-associated kinase activation. (ntu.edu.tw)

We have discovered that Smac mimetics can induce long-term changes in the breast cancer cell phenotype which includes inflammatory signaling. (lu.se)

Only more recently has it become clear that apoptotic cells are the source of signals that can have profound effects on their neighbors. (elifesciences.org)
The anti-proliferative and apoptotic effects of the perifosine+vitamin D combination (30 μM + 200 nM at 48 h and 10 μM + 200 nM at 72 h) on HEC-1A cells were higher than in perifosine and vitamin D alone. (excli.de)
GPI1046 and V10367, non-immunosuppressive IPLs (NI-IPLs), as well as FK506, an immunosuppressive IPL (I-IPL), had cytoprotective effects against hydrogen peroxide (H 2 O 2 )-induced apoptotic cell death in U251 cells. (elsevierpure.com)
In U251 cells, FK506, GPI1046 and V10367 had GSH-activating and NTF-activating effects. (elsevierpure.com)
To determine whether resveratrol, a natural plant-derived drug, has protective effects against antibody-induced apoptosis of retinal cells in vitro and to provide insights on the mechanism of resveratrol protection. (biomedcentral.com)
The protective effects of resveratrol on the retinal cells were examined in the in vitro study using undifferentiated, immortalized rat retinal E1A.NR3 cells, which express markers specific for photoreceptors, bipolar cells, ganglion cells, and retinal glial cells [ 19 ]. (biomedcentral.com)
Studies have also shown that ellagic acid may protect against chromosome damage and reduce the effects of chemically induced hepatic structural degeneration. (nutrimedical.com)
Baicalin treatment reduced phosphorylation levels of PKCδ and p53, as well as the expression levels of active caspase3 and bax in HAECs exposed to H/R. The treatment of PKC activator PEP005 impaired the protective effects of baicalin in increasing cell viabilities and reducing apoptosis in HAECs exposed to H/R. (medscimonit.com)
In the present study, we investigated the anticancer effects of adenine and the underlying mechanism in colon cancer cells. (hindawi.com)

CATH.a cells were significantly protected by the addition of 5 mM GSH (Mn EC50=200 M) and 10 mM N-acetyl cysteine (NAC) (Mn EC50=300 M), therefore, indirectly identifying intracellular ROS formation as a mechanism for Mn neurotoxicity. (cdc.gov)

This study investigated whether hypoxic OM-MSCs could inhibit microglial cell death upon I/R insult in vitro . (aging-us.com)

In this study, we evaluated the effect of resveratrol, a polyphenolic phytoalexin, on levels of [Ca +2 ] i and on protection of retinal cells from antibody-induced apoptotic death in vitro . (biomedcentral.com)
MLE-12 cells were induced by LPS to construct an ARDS model in vitro. (karger.com)

The cyclin-dependent kinase inhibitor, olomoucine, prevents cell cycle progression and resveratrol-induced apoptosis. (lacienciadelvino.com)
El resveratrol (trans-3, 4 ',5-trihidroxiestilbeno) es un compuesto natural altamente encontrado en las uvas, cacahuates, vino tinto, y una variedad de fuentes de alimento. (lacienciadelvino.com)
Nuestros datos muestran que el resveratrol induce la dosis y la muerte en función del tiempo de las células U251, medida por la liberación de lactato deshidrogenasa y i ensayos de fragmentación del ADN. (lacienciadelvino.com)
El resveratrol induce la activación de la caspasa-3 y aumenta la división del sustrato de caspasa aguas abajo, la polimerasa poli (ADP-ribosa). (lacienciadelvino.com)
El resveratrol induce la liberación del citocromo c desde la mitocondria al citoplasma y la activación de la caspasa-9. (lacienciadelvino.com)
El resveratrol inhibe la proliferación U251, según lo medido por el ensayo MTS [3 - (4,5-dimetiltiazol-2-il) -5 - (3-carboxymethoxyphenyl) -2 - (4-sulfonatofenil)-2H-tetrazolio, interior sal], y induce G0/G1, según lo determinado por citometría de flujo. (lacienciadelvino.com)
Estos resultados sugieren que las múltiples vías de señalización pueden dar lugar a la muerte apoptótica de U251 por el resveratrol, que garantiza una mayor exploración como un agente contra el cáncer en gliomas humanos. (lacienciadelvino.com)
E1A.NR3 retinal cells pretreated with 40 μM resveratrol were grown in the presence of anti-recoverin (Rec-1), anti-enolase (Enol-1) antibodies, and normal purified immunoglobulins. (biomedcentral.com)
When the cells were exposed to resveratrol before treatment with Enol-1 or Rec-1 antibodies, 30-55% more cells survived compared to the resveratrol-untreated cells. (biomedcentral.com)
Sirt1 expression was greatly reduced in the cells grown in the presence of Rec-1 and Enol-1, but it increased about five times in the resveratrol-pretreated cells. (biomedcentral.com)
Immunocytochemistry revealed that Sirt1 expression in the cytoplasm and nucleus was colocalized with Ku70 expression in resveratrol-treated cells, suggesting possible interaction with each other in the cell. (biomedcentral.com)

However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
Complement, a complex system of enzymes and regulators endowed with multiple biological activities (opsonisation, chemoattraction of leukocytes, cell lysis, cell activation), is a major component of innate defence against pathogens. (europa.eu)
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1a induced cell death. (lu.se)
The resulting aggregate called death-inducing signaling complex (disc) performs CASP8 proteolytic activation. (lu.se)

It prevents cancer cells from reproducing. (911weknow.com)

Activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) were measured in ellagic acid-treated V79-4 cells. (nutrimedical.com)
Recent studies have demonstrated the potential of pesticides to either inhibit or induce xenobiotic metabolizing enzymes in humans. (cdc.gov)

Anatomy36

Organisms12

Diseases9

Chemicals and Drugs126

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes49

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science3

Health Care1

Programmed cell5

Leading to apoptotic cell2

Cytotoxicity7

Pathway19

Proliferation17

Pathways9

Protein24

Caspase25

Death induced7

Clearance of apoptotic cells3

Anti-apoptotic effects2

Blocking TNF inhi1

Reactive oxygen2

Inhibition3

Necrotic8

Mechanisms12

Viability14

Flow cytometry4

Hypoxia6

Upregulation3

Downstream3

Toxicity3

Bodies4

HepG29

Abstract1

Mesenchymal stem3

Extracellular3

Fragmentation4

Pharmacological2

Proteins6

Exposure4

Human19

Cisplatin-induced1

Endothelial Cell Apoptosis1

Cycle arrest2

Acute3

Assays2

Cancerous1

Mitochondrial3

Receptors4

MRNA3

Molecular1

Ligands1

Vivo5

Keratinocytes2

Potent2

Lipid peroxidation products2

Hepatocyte1

FASL2

Responses4

Chromatin condensation1

Cytotoxic2

Necrosis2

Epidermal1

Cellular2

Compound2

Arrest1

Oxidative1

Nuclear6

Inflammatory1

Effects9

Intracellular1

Microglial1

Vitro2

Resveratrol11

Activation4

Prevents1

Enzymes2