Induces acrosome reaction. Medical search. Frequent questions

In this paper, we describe the capacitation phenotype of sperm lacking the long isoform of β1,4-galactosyltransferase I (GalT I), a sperm surface protein that functions as a receptor for the zona pellucida glycoprotein, ZP3, and as an inducer of the acrosome reaction following ZP3-dependent aggregation. (biologists.com)
As expected, wild-type sperm must undergo capacitation in order to bind the zona pellucida and undergo a Ca 2+ ionophore-induced acrosome reaction. (biologists.com)
By contrast, GalT I-null sperm behave as though they are precociously capacitated, in that they demonstrate maximal binding to the zona pellucida and greatly increased sensitivity to ionophore-induced acrosome reactions without undergoing capacitation in vitro. (biologists.com)
Sperm Capacitation and Acrosome Reaction in Mammalian Sperm. (umassmed.edu)
In addition to its PRKA-dependent effects, different studies have clearly demonstrated that cAMP also activates EPAC ( E xchange P roteins directly A ctivated by c AMP) during capacitation and acrosome exocytosis [ 15 - 17 ]. (plos.org)
Pretreatment with genistein (50 and 250 micromol/l) decreased capacitation-induced tyrosine phosphorylation and resulted in a loss of phosphorylation in response to progesterone treatment. (dundee.ac.uk)
In spermatozoa, several key functions are regulated by cytoplasmic Ca2+ concentration such as sperm capacitation, chemotaxis, hyperactive motility, and acrosome reaction. (123dok.net)
To get to the story -- which is engaging -- readers must trod through all manner of OB/GYN terminology: "Clomiphene citrate," "ovulation induction," "reproductive endocrinology," "hysterosalpingogram," "Fallopian tubes," "intrauterine insemination," "capacitation," "zona pellucida," and "ligand-induced acrosome reaction. (columbiaheartbeat.com)
8-Br-2-O-Me-cAMP prompted an increase in Rap1 activation, in the scrambling of plasma membrane phospholipids (necessary for the capacitation process), the acrosome reaction, motility, and calcium mobilization, when spermatozoa were incubated in acrosome reaction conditions. (researchensemble.com)
Phospholipase C zeta undergoes dynamic changes in its pattern of localization in sperm during capacitation and the acrosome reaction. (ox.ac.uk)
CONCLUSION(S): Phospholipase C zeta is localized to acrosomal and post-acrosomal regions and undergoes dynamic changes during capacitation and the acrosome reaction, indicating a potential role regulating not only egg activation but other sperm functions. (ox.ac.uk)

Binding to the zona pellucida clusters the sperm receptor, activating intracellular signaling cascades that stimulate the exocytosis of the acrosome. (biologists.com)
Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca2+ mobilization in the zona pellucida-induced acrosome reaction. (joplink.net)
The mammalian sperm acrosome reaction entails extensive fusion between the periacrosomal plasma membrane and the outer acrosomal membrane, with the release of hydrolases which function in sperm penetration through the zona pellucida [1-3] . (ommegaonline.org)

This structure binds spermatozoa, and is required to initiate the acrosome reaction. (wikipedia.org)
They bind to capacitated spermatozoa and induce the acrosome reaction. (wikipedia.org)
We also reveal using a combination of immunological techniques and proteomics analytical tools that the PDE10A isoform X4 is present in the area of the developing acrosome of spermatids and of the acrosome of mature spermatozoa. (plos.org)
Previous studies have implicated receptor tyrosine kinases in progesterone-induced [Ca2+]i signalling, and consequent induction of the acrosome reaction, in human spermatozoa. (dundee.ac.uk)
Induction of the acrosomal reaction in mouse spermatozoa by the solution of pellucid zone from alpaca (Lama pacos L. (edu.pe)
Dive into the research topics of 'Induction of the acrosomal reaction in mouse spermatozoa by the solution of pellucid zone from alpaca (Lama pacos L.)'. Together they form a unique fingerprint. (edu.pe)
The objective of the present study is to identify and localize syntaxin in bovine epididymal spermatozoa and to determine the fate of syntaxin 2 during the acrosome reaction. (ommegaonline.org)
These diverse functions performed by the acrosomal segment during fertilization reveal the importance of identifying the specific signaling events and protein constituents which initiate and regulate the membrane fusion process of the acrosome reaction and prepare spermatozoa to fuse with the egg. (ommegaonline.org)
Abstract] The acrosome reaction is a highly regulated exocytotic event that primes spermatozoa for successful fertilization. (bio-thing.com)
Having shed their acrosome, spermatozoa are then capable of penetrating the outer vestments of the oocyte and initiating fertilization. (bio-thing.com)
Accordingly, the failure of spermatozoa to complete an acrosome reaction represents a relatively common etiology in male infertility patients, and the ability to induce acrosomal exocytosis has found clinical utility in the evaluation of sperm fertilizing capacity. (bio-thing.com)

Our immunoblot data reveals that syntaxin 2 of bovine cauda sperm is released after lysophosphatidylcholine (LPC)-induced acrosomal exocytosis. (ommegaonline.org)
The acrosome reaction is analogous to regulated exocytosis in somatic cells in that both are initiated by ligand acting at the plasma membrane, both require activation of signaling pathways and ion channels, both are Ca 2+ -dependent and both result in the fusion of the plasma membrane with the membrane of a docked secretory granule (acrosome) [2,9,10] . (ommegaonline.org)

Starvation induces an increase in intracellular calcium and potentiates the progesterone-induced mouse sperm acrosome reaction. (umassmed.edu)
However, we could detect no effect of genistein on progesterone-induced [Ca2+]i signalling. (dundee.ac.uk)
We conclude that neither the transient nor sustained phases of the progesterone-induced [Ca2+]i response require receptor tyrosine kinase signalling. (dundee.ac.uk)
Previous reports of modulation of the progesterone-induced [Ca2+]i signal by tyrosine kinase inhibition probably reflect inhibition of the acrosome reaction. (dundee.ac.uk)
Thus, in amphibians, it has been assumed for a long time, that the actinomycin D-insensitive progesterone-induced oocyte maturation [ 4 ] was mediated by a membrane steroid receptor rather than a nuclear receptor. (biomedcentral.com)

Involvement of zinc in the regulation of pHi, motility, and acrosome reactions in sea urchin sperm. (rupress.org)
In the presence of a variety of structurally different metal chelators (0.1-1 mM EDTA, EGTA, phenanthroline, dipyridyl, cysteine, or dithiothreitol), motility initiation is delayed and the acrosome reaction is inhibited. (rupress.org)
Since both sperm motility and acrosome reactions are in part regulated by intracellular pH (pHi), the effect of chelators on the sperm pHi was examined by using the fluorescent pH sensitive probe, 9-aminoacridine, EDTA depresses sperm pHi in both species, and 0.1 microM free Zn+2 reverses this pHi depression. (rupress.org)
When sperm are diluted into media that contain chelators, both NH4Cl and monensin (a Na+/H+ ionophore) increase the sperm pHi and reverse the chelator inhibition of sperm motility and acrosome reactions. (rupress.org)
The results of this study are consistent with the involvement of a trace metal (probably zinc) in the pHi regulation of sea urchin sperm and indicate a likely mechanism for the previously observed effects of chelators on sperm motility and acrosome reactions. (rupress.org)

Na(+) influx via Orai1 inhibits intracellular ATP-induced mTORC2 signaling to disrupt CD4 T cell gene expression and differentiation. (nih.gov)

The present study documents distinctive features of the cumulus oophorus before and after ovulation, and of copulation-induced maturation of the ovulatory follicle, which has no antrum. (elsevierpure.com)
In lower vertebrates, steroid-induced oocyte maturation is considered to involve membrane-bound progestin receptors. (biomedcentral.com)
In addition, the mRNA abundance of both genes is not increased after in vitro hormonal stimulation of full grown follicles by maturation inducing hormones. (biomedcentral.com)
Together, our findings suggest that PGMRC1 is a new possible participant in the progestin-induced oocyte maturation in fish. (biomedcentral.com)
This second type of steroid action has been proposed to be involved in steroid-induced oocyte maturation in lower vertebrates. (biomedcentral.com)

ZP3 is then involved in the induction of the acrosome reaction, whereby a spermatozoon releases the contents of the acrosomal vesicle. (wikipedia.org)
We focused on induction of the acrosome reaction as a key process in fertilization. (unl.edu)
A. amurensis and A. forbesi belong to the same induction group of the acrosome reaction. (unl.edu)
Fragment 1 plays an important role in the subfamily specificity of acrosome reaction induction. (unl.edu)
These results demonstrate that the specificity of acrosome reaction induction is present at the subfamily level in sea stars. (unl.edu)
The aim of this study was to study the induction of the acrosomal reaction in mouse sperms with homologous-Pellucid Zone and heterologous-Pellucid Zone. (edu.pe)
All the above inhibitors were procured from Sigma Aldrich Inc. Statistical analysis The results pertaining to SIZP mediated induction of acrosome reaction Inhibitors,Modulators,Libraries are presented as mean SEM and statistical analysis was done by comparing the means of the medium control vehicle control and e perimental sets or within two e perimental groups by using paired Students t test Wilco on signed rank test. (vegfr-3inhibitor.com)
Results SIZP induces acrosomal e ocytosis in capacitated human sperm in a dose dependent manner A significant increase in the induction of acrosomal e o cytosis of capacitated human sperm was observed with a concomitant increase in number of zonae equivalent used per reaction, Inhibitors,Modulators,Libraries as compared to PBS. (vegfr-3inhibitor.com)
As low as 1 zona equivalent was able to induce statistical significant induction of acrosome reaction in capacitated human sperm. (vegfr-3inhibitor.com)
Capaci tated sperm prepared from 6 different donors on incu bation with optimized concentration of human SIZP showed a significant increase in induction Inhibitors,Modulators,Libraries of acrosome reaction. (vegfr-3inhibitor.com)
T type VOCCs are responsible for SIZP mediated induction Inhibitors,Modulators,Libraries of acrosome reaction subsequent to an initial increase in i An increase in i, after coming in contact with ZP, is a prerequisite for induction of acrosomal e ocytosis in mammalian sperm. (vegfr-3inhibitor.com)
To decipher the type of VOCCs playing an important role in human SIZP mediated increase in initial i surge as well as subsequent induction of acrosome reaction, pharmacological inhibitors for Brefeldin_A L and T type VOCCs were employed. (vegfr-3inhibitor.com)
To further assess the importance of these VOCCs, induction of acrosome reaction in capacitated human sperm was quantitated after incubation with SIZP in presence or absence of pharmacological inhibitors of L or T type specific VOCC. (vegfr-3inhibitor.com)
these adverse events occurred with higher rates in non-induced patients, it is likely that induction alone is not the: primary risk determinant for PTLD and infection. (sirtuinsignaling.com)
Studies presented in this manuscript suggest that in humans, ZP mediated induction of acrosome reaction is also inhibited by inhibitor of GABAA receptor. (ainhibitor.com)

Role of T type VOCCs was further reinforced by inhibition of acrosome reaction mediated by human SIZP in presence Inhibitors,Modulators,Libraries of two different T type VOCCs inhibitors. (ainhibitor.com)
Further, chelating the e tracellular calcium by EGTA also led to inhibition of SIZP mediated acrosome reac tion. (ainhibitor.com)

Therefore, steps after the acrosome reaction are responsible for the species specificity. (unl.edu)
To explain acrosome reaction subfamily specificity at the molecular level, the sugar components of egg jelly were examined and analyzed by principal component analysis. (unl.edu)
Obtained results indicate a potential role of VFAs in the olfactory guided behavior of A. diaperinus, we speculate that reaction to the presence if VFAs may deviate form specificity of species' original habitat. (preprints.org)
These polysaccharides show species specificity in inducing the acrosome reaction in sea urchin sperm, providing a clear-cut. (embrapa.br)

Acrosome reaction is subfamily specific in sea star fertilization" by Mia Nakachi, Hideaki Moriyama et al. (unl.edu)
In the fertilization process of sea stars, sperm is activated to go through the acrosome reaction before cell fusion. (unl.edu)
The musk shrew, Suncus murinus, is an induced ovulator, the cumulus oophorus of which is unusual in several respects: it has no matrix, it always induces the acrosome reaction and it appears to be essential for fertilization. (elsevierpure.com)
The equatorial segment of the acrosome underlies the domain of the sperm that fuses with the egg membrane during fertilization. (bioone.org)
The successfulness of mammalian fertilization completely relies on the efficacy of the acrosome reaction. (ommegaonline.org)
Therefore, the increase in the acrosome reaction, together with the increase in calcium (which is known to be essential for fertilization) and the Epac nteraction with E-cadherin, might indicate that Epac proteins have an important role in gamete recognition and fertilization. (researchensemble.com)

Calcium signalling plays a pivotal role in sperm physiology, being intimately involved in the regulation of acrosome reaction, chemotaxis and hyperactivation. (dundee.ac.uk)
All of these compounds induce calcium signals in the posterior sperm head and neck region and, when applied at appropriate concentrations, modify flagellar activity, causing asymmetric bending of the proximal flagellum. (dundee.ac.uk)

Sukhorum W, Arun S, Burawat J, Sumpannang A, Iamsaard S. Expressions of Testicular Steriodogenic Proteins and Tyrosine Phosphorylation in VPA-induced Rats. (mfu.ac.th)

Sukhorum W , Iamsaard S. Changes of testicular function proteins and sperm acrosome status in rats treated with valproic acid. (mfu.ac.th)
Arun S, Burawa J, Sukhorum W, Sampannang A, Maneenin C, Iamsaard S. Chronic restraint stress induces sperm acrosome reaction and changes in testicular tyrosine phosphorylated proteins in rats. (mfu.ac.th)
Although the members of Q-SNARE proteins are characterized in somatic cells, it is not known whether related proteins function in the sperm acrosome reaction. (ommegaonline.org)
Although the members of these protein families are widely distributed among various types of somatic cells [10,11] , it is not known whether related proteins function in the acrosome reaction. (ommegaonline.org)
Finally, the activation of Epac proteins induced a change in the distribution of E-cadherin. (researchensemble.com)

In contrast to the sperm acrosome reaction, the secretory pathway of somatic cells has received detailed characterization in recent years and a great deal has been learned of the processes regulating vesicle docking and fusion with the plasma membrane. (ommegaonline.org)

Homotypic fusion among echinoderm egg yolk granules has previously been reconstituted in vitro, and shown to be a rapid, Ca2+-triggered reaction that can produce extremely large (>10 microm diameter) fusion products. (lookformedical.com)

The cumulus is stabilized by gap and tight junctions, and presents a smooth external surface that appears to initiate the acrosome reaction. (elsevierpure.com)

In contrast to T type VOCCs inhibitors, L type VOCCs inhibitors failed to inhibit SIZP mediated acrosome reaction. (ainhibitor.com)

Cofactor for acrosome reaction-inducing substance (Co-ARIS) is a steroidal saponin from the starfish Asterias amurensis. (elsevierpure.com)
Enzyme-linked immunosorbent assays indicate that Asteriinae subfamily share a common glycan structure, the Fragment 1 of Acrosome Reaction-Inducing Substance from A. amurensis . (unl.edu)

Instead, may protect sperm from premature acrosome reaction in the epididymis by binding to lipid peroxides, which might otherwise interact with phospholipase A2 and induce the acrosome reaction. (joplink.net)
Sperm cell membranes are high in polyunsaturated fatty acids and are sensitive to oxygen free radical induced damage mediated by lipid peroxidation. (scialert.net)
Reactive oxygen species (ROS) induced lipid peroxidation is associated with sperm function. (ijbs.com)

Sampannang A, Sripanidkulchai B. Protective effect of Momordica cochinchinensis (L.) Spreng aril extract on essential testicular markers in rats induced with valproic acid. (mfu.ac.th)
Sukhorum W , Sampannang A, Sripanidkulchai B, Iamsaard S. Momordica cochichinesis (L.) Spreng aril extract prevents adverse reproductive parameters of male rats induced with valproic acid. (mfu.ac.th)

Following the acrosome reaction the fused hybrid membrane complex remains adherent to the zona surface, [4] and the newly exposed inner acrosomal membrane of the spermatozoon functions as the limiting membrane or plasma membrane equivalent [1] . (ommegaonline.org)

Sampannang A, Arun S, Burawat J, Sukhorum W, Iamsaard S. Testicular histopathology and phosphorylated protein changes in mice with diabetes induced by multiple-low doses of streptozotocin: An experimental study. (mfu.ac.th)

Anacetrapib Heat solubilized human ZP mediated acrosome reac tion involves activation of Gi protein coupled receptor pathway which is in concordance with previous reports. (ainhibitor.com)

Granule washing with mildly chaotropic salt abolished this tethering reaction, and prevented Ca2+-triggered formation of the large fusion products characteristic of tethered granules. (lookformedical.com)

Sperm from hyh mice carrying a point mutation in alphaSNAP have a defect in acrosome reaction. (nih.gov)
Gac) aril extract on reproductive damages in streptozotocin (STZ)-induced hyperglycemia mice. (mfu.ac.th)
CRC (Colorectal cancer) cell lines did not induce robust stromal TGF-beta responses when injected into nude mice as shown by lack of p-SMAD2 accumulation in tumor-associated stromal cells. (bio-thing.com)

Picroto in a GABAA receptor inhibitor, inhibits pro gesterone as well as recombinant human ZP3 fragment mediated acrosome reaction. (ainhibitor.com)

Iamsaard S, Sukhorum W, Arun S, Phunchago N, Uabundit N, Boonruangsri P, Namking M. Valproic acid induces histologic changes and decreases androgen receptor levels of testis and epididymis in rats. (mfu.ac.th)

It is assumed that syntaxin 2 may be involved in triggering the acrosome reaction through a ligand-receptor mediated signal transduction pathway. (ommegaonline.org)

Egg water from the amphibian Bufo arenarum modulates the ability of homologous sperm to undergo the acrosome reaction in the presence of the vitelline envelope. (umassmed.edu)

Acrosome reaction assays indicate that the acrosome reaction can be induced across species within Asteriinae subfamily. (unl.edu)

Conclusions: The photo-induced bactericidal efficacy of TiO(2) films is dependent on their surface characteristics. (srcinhibitors.com)

Light microscopic immunofluorescent observations revealed that during acrosome biogenesis ESP first appears in the nascent acrosomal vesicle in early round spermatids and subsequently segregates to the periphery of the expanding acrosomal vesicle, thereby defining a peripheral equatorial segment compartment within flattened acrosomal vesicles and in the acrosomes of early and late cap phase, elongating, and mature spermatids. (bioone.org)
Based on these observations, we propose a new model of acrosome biogenesis in which the equatorial segment is defined as a discrete domain within the acrosomal vesicle as early as the Golgi phase of acrosome biogenesis. (bioone.org)

However, no further increase in acrosomal e o cytosis was observed with SIZP preparation from more than 5 zonae per reaction. (vegfr-3inhibitor.com)
However, the ability of SIZP prepared from fer tilized eggs to induce acrosome reaction needs further investigation. (ainhibitor.com)

to prevent polyspermy, and enable the acrosome reaction for the successful adhesion and penetration by the sperm cell. (wikipedia.org)

Patrat et al, has shown that solubilized zona prepared from unfertilized and fertilized human eggs induces acrosome reaction and increase in i is mediated by T type VOCC. (ainhibitor.com)

Research in the department has shown that season significantly affects the sperm features in the Barbari bucks and summer season induces more DNA damage as compared to winter and rainy seasons. (edu.in)
Using polymerase chain reaction Mycoplasma hominis and Ureaplasma urealyticum were detected in cervical secretions of 19.2% and 13.7% of infertile women, and the presence of mycoplasma was significantly correlated with the presence of AZP-Ab and ASA. (who.int)

GABA also induces acrosome reaction in human sperm. (ainhibitor.com)
The present study was conducted to determine the effect of two human chorionic gonadotropin (hCG) treatments on the fertility of ewes which were estrus-induced and artificially inseminated during the non-breeding season. (go.jp)

Anatomy18

Organisms13

Diseases1

Chemicals and Drugs36

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes15

Capacitation11

Zona pellucida3

Spermatozoa11

Exocytosis2

Progesterone-induced5

Motility5

Intracellular1

Maturation5

Induction15

Inhibition2

Specificity4

Fertilization6

Calcium2

Tyrosine phosphorylation1

Proteins5

Secretory1

Vitro1

Initiate1

Inhibitors1

Amurensis2

Lipid3

Rats induced2

Spermatozoon1

Testicular1

Protein1

Granule1

Mice3

Fragment1

Epididymis1

Receptor1

Undergo1

Species1

Efficacy1

Acrosomal vesicle2

SIZP2

Penetration1

Increase1

Significantly2

Human2