• Scholars@Duke publication: Inhibition of Isoprenylcysteine Carboxylmethyltransferase Induces Cell-Cycle Arrest and Apoptosis through p21 and p21-Regulated BNIP3 Induction in Pancreatic Cancer. (duke.edu)
  • As these proteins are also involved in the regulation of apoptosis in many circumstances, we investigated their contribution to other relevant TGF beta-induced effects, namely apoptosis and EMT, and examined how the various processes were interrelated. (st-andrews.ac.uk)
  • TGF beta induced high levels of apoptosis in p53-/-, Rb-/-, p21(cip1)-/- and control hepatocytes although with slight differences in kinetics. (st-andrews.ac.uk)
  • However, hepatocytes deficient in both p53 and p21(cip1)were less sensitive to TGF beta-induced apoptosis. (st-andrews.ac.uk)
  • Conclusion: Although p53, p21(Cip1) and pRb are well known regulators of both proliferation and apoptosis in response to a multitude of stresses, we conclude that they are critical for TGF beta-driven inhibition of hepatocytes proliferation, but only slightly modulate TGF beta-induced apoptosis. (st-andrews.ac.uk)
  • High concentrations of Salinomycin induced a G2 arrest, downregulation of survivin and triggered apoptosis. (uaeu.ac.ae)
  • In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
  • These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
  • We previously found that carbamate pesticides induced significant apoptosis in human natural killer cells. (researchgate.net)
  • To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or ziram. (researchgate.net)
  • Gain of function and loss of function experiments showed that PDCD4 induced turnover (proliferation and apoptosis) of HUVECs. (plos.org)
  • PDCD4 induces turnover (proliferation and apoptosis) of HUVECs. (plos.org)
  • A JNK-dependent pathway is required for TNF α -induced apoptosis. (nature.com)
  • Proteinase 3 sidesteps caspases and cleaves p21(Waf1/Cip1/Sdi1) to induce endothelial cell apoptosis. (mpg.de)
  • Inhibiting PAK1 signals induce tumor cell apoptosis (cell death). (thehealthyplanet.com)
  • Additionally, JIB extract induced B16/F10 cell apoptosis via the caspase cascade. (medsci.org)
  • These findings indicate that the doenjang attenuated colon carcinogenesis induced by AOM and DSS by ameliorating the symptoms of colon cancer, reducing the occurrence of neoplasia, regulating proinflammatory cytokine levels, and controlling the expressions of inflammation- and apoptosis-related genes in the colonic tissue. (carcinogenesis.com)
  • KEGG enrichment analysis showed that FO mainly regulates PI3K-AKT- and apoptosis-related signals, in which BBC3, DDIT3, NOXA, and CDKN1A on the surface serve as the novel targets of FO inducing HCC cell apoptosis. (hindawi.com)
  • N- trans -Feruloyloctopamine (FO) is the extract of garlic skin, which possesses high antioxidant activity and selectively induces cell apoptosis in leukemia tumors [ 6 ]. (hindawi.com)
  • Firstly, the overexpression of miRNA‑375 significantly suppressed cell proliferation, increased lactate dehydrogenase activity and induced apoptosis in HPV‑18(+) cervical cancer cells. (spandidos-publications.com)
  • Previously, we have reported that exposure of BEAS-2B cells to single wall carbon nanotube s (SWCNT) caused ultra-structural and morphological changes, cytotoxicity, apoptosis, and induced oxidative stress as shown by the formation of free radicals, accumulation of peroxidative products, and depletion of antioxidants. (cdc.gov)
  • TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
  • We have recently reported that P53, P21(Cip1) and pRB, three critical regulators of the G1/S transition are variably involved in TGF beta-induced cell cycle arrest in hepatocytes. (st-andrews.ac.uk)
  • Interestingly, treatment with low concentrations of Salinomycin induced a transient G1 arrest at earlier time point and G2 arrest at later point and senescence associatedwith enlarged cellmorphology, upregulation of p21 protein, increase in histone H3 and H4 hyperacetylation and expression of SA-β-Gal activity. (uaeu.ac.ae)
  • SnCs exhibit irreversible growth arrest accompanied by increased expression of cyclin-dependent kinase inhibitors (CDKi) such as p16 INK4a , and p21 Cip1 , accumulation of DNA damages, and secretion of diverse bioactive molecules known as the senescence-associated secretory phenotype (SASP). (aging-us.com)
  • However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. (nih.gov)
  • Oncostatin M induces growth arrest by inhibition of Skp2, Cks1, and cyclin A expression and induced p21 expression. (mpg.de)
  • A secreted low-molecular-weight protein from Helicobacter pylori induces cell-cycle arrest of T cells. (mpg.de)
  • The combined treatment induced significant mitotic arrest and activated the apoptotic cascades, tumor cell death. (thehealthyplanet.com)
  • JIB extract induced cell cycle arrest at the G 0 /G 1 phase and decreased cyclin and cdk protein expressions. (medsci.org)
  • We have shown previously that perifosine induces p21(waf1/cip1) in a p53-independent fashion and that induction of p21(waf1/cip1) is required for the perifosine-induced cell cycle arrest because cell lines lacking p21(waf1/cip1) are refractory to perifosine. (conicet.gov.ar)
  • Activation of p21 or p16 therefore causes cell cycle arrest. (medscape.com)
  • This effect may depend on other parameters such as proliferation and the presence of other regulatory proteins as suggested by the consequences of p53, p21(Cip1) double deficiency. (st-andrews.ac.uk)
  • Similarly, p53, p21(Cip1) and pRB deficiency had no effect on the morphological changes and loss of cell adhesion which is thought to be critical for metastasis. (st-andrews.ac.uk)
  • Transcriptional activation of p21(waf1/cip1) by alkylphospholipids: role of the mitogen-activated protein kinase pathway in the transactivation of the human p21(waf1/cip1) promoter by Sp1. (conicet.gov.ar)
  • In this report, we investigated the mechanism by which perifosine induces p21(waf1/cip1) protein expression. (conicet.gov.ar)
  • We observed that perifosine induces the accumulation of p21(waf1/cip1) mRNA without affecting p21(waf1/cip1) mRNA stability. (conicet.gov.ar)
  • Using several p21(waf1/cip1) promoter-driven luciferase reporter plasmids, we observed that perifosine activates the 2.4-kb full-length p21(waf1/cip1) promoter as well as a p21 promoter construct lacking p53-binding sites, suggesting that perifosine activates the p21(waf1/cip1) promoter independent of p53. (conicet.gov.ar)
  • Mutations in each particular Sp1 site block perifosine-induced p21(waf1/cip1) expression. (conicet.gov.ar)
  • Ablation of p21-activated kinase-1 in mice promotes isoproterenol-induced cardiac hypertrophy in association with activation of Erk1/2 and inhibition of protein phosphatase 2A. (ox.ac.uk)
  • Earlier investigations in our lab indicated an anti-adrenergic effect induced by activation of p21-activated kinase (Pak-1) and protein phosphatase 2A (PP2A). (ox.ac.uk)
  • We have recently shown that tumor necrosis factor (TNF) α induces caspase-dependent and -independent JNK activation and ROS accumulation in cellular FLICE-inhibitory protein (c-Flip) −/− murine embryonic fibroblasts (MEFs). (nature.com)
  • Furthermore, TNF α and Fas induced the cleavage of mitogen-activated protein kinase/ERK kinase kinase (MEKK)1, resulting in generation of a constitutive active form of MEKK1 leading to JNK activation in c-FLIP knockdown cells. (nature.com)
  • p27 binds cyclin-CDK complexes through a sequential mechanism involving binding-induced protein folding. (mpg.de)
  • Does not affect the activity of p21-activated protein kinase (PAK) even at high concentrations (~100 µM). (sigmaaldrich.com)
  • The overexpression of miRNA‑375 significantly increased caspase‑3 and caspase‑9 activities, induced B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein, tumor protein 53 and cyclin‑dependent kinase inhibitor 1 protein expression and suppressed cyclin D1 and survivin protein expression in HPV‑18(+) cervical cancer cells. (spandidos-publications.com)
  • Moreover, we showed that perifosine activates the mitogen-activated protein/extracellular signal-regulated kinase pathway, and this activation promotes the phosphorylation of Sp1 in known mitogen-activated protein kinase residues (threonine 453 and 739), thereby leading to increased Sp1 binding and enhanced p21(waf1/cip1) transcription. (conicet.gov.ar)
  • To suppress this antiapoptotic function of NF- κ B might be a promising strategy to increase susceptibility of tumor cells to stress-induced cell death. (nature.com)
  • Consistent with the results using c-Flip −/− MEFs, we found that TNF α stimulation induced caspase-dependent prolonged JNK activation and ROS accumulation, followed by apoptotic and necrotic cell death in various tumor cells. (nature.com)
  • The p21 activated Kinase PAK1 is implicated in tumor genesis. (thehealthyplanet.com)
  • Oncogene-induced senescence (OIS) is a tumor suppression mechanism that blocks cell proliferation in response to oncogenic signalling. (nih.gov)
  • CWR22Rv1 cells, a castrate-resistant prostate cancer cell line, were treated with Zyflamend and the expression of class I and II histone deacetylases, along with their downstream target the tumor suppressor gene p21, was investigated. (biomedcentral.com)
  • Expression of the tumor suppressor gene p21, a known downstream target of histone deacetylases and CBP/p300, was increased by Zyflamend treatment and the effect on p21 was, in part, mediated through Erk1/2. (biomedcentral.com)
  • In vivo , CWO induced transcriptional changes in immune-related genes, resulting in cytotoxic T cell-dependent tumor regression. (biorxiv.org)
  • Here, we show that the NFκB pathway directly activates expression of the proliferation inhibitor p21 in response to DNA damage in myeloid leukemia cells. (psu.edu)
  • Our results uncover a p53-independent pathway for p21 activation involved in controlling hematopoietic cell fate. (psu.edu)
  • Pulsatile shear stress induces ubiquitin-proteasome-mediated degradation of PDCD4 via a PI3K/Akt pathway in HUVECs. (plos.org)
  • These results revealed that NDRG1 is functional in prostate cancer cells and able to induce expression of differentiation factors through p53 independent pathway. (scirp.org)
  • However, the pathway downstream NDRG1, involving p21 and c8/18, regulating cell cycle progression and DNA synthesis is unfunctional. (scirp.org)
  • In addition, the p21 mRNA level was elevated in 1,4-BQ-treated cells, suggesting that human CD34(+) cells utilize the p53 pathway in response to 1,4-BQ-induced DNA damage. (rti.org)
  • We propose that activated Ras induces transcriptional changes that predispose cells undergoing OIS to mitotic stress and multinucleation. (nih.gov)
  • We found that NFκB-mediated p21 activation controls DNA damage-induced myeloid differentiation. (psu.edu)
  • The cyclin-dependent kinase (CDK) inhibitors p21 and p16 inhibit the activity of CDKs, such as CDK4. (medscape.com)
  • Here, we studied the synaptic expression mechanisms for deprivation-induced weakening of L4-L2/3 inputs and assessed its similarity to LTD, which is known to be expressed presynaptically at L4-L2/3 synapses. (jneurosci.org)
  • Immunoprecipitation revealed that pulsatile shear stress induced the coupling of ubiquitin with PDCD4 expression. (plos.org)
  • Chronic exposure to IL-1β induces only partial expression of senescence markers and does not allow us to conclude on its ability to induce senescence in chondrocytes. (aging-us.com)
  • On the other hand, etoposide treatment reliably induces DNA damage-related senescence in human articular chondrocytes evidenced by loss of proliferative capacity, DNA damage accumulation, and expression of some SASP components. (aging-us.com)
  • Also our study indicated that WSC induced an increase in the transcriptional expression of matrix metalloproteinases, MMP-2 and MMP-9 and an immune response regulator, Toll Like Receptor-4. (nih.gov)
  • In addition, it increased Bax and reduced Bcl-2 expression levels and increased p21 and p53 expression in the colonic tissues. (carcinogenesis.com)
  • The study assessed the genetic expression patterns of chemically treated cells compared to aged human-origin cells and OSK(MYC)-induced murine and human iPSC. (news-medical.net)
  • This resulted in over-expression of NDRG1, which in turn markedly up regulated two differentiation markers of the prostate tissue, p21 and cytokeratin 8/18. (scirp.org)
  • Involvement of p21 was confirmed with siRNA knockdown and over expression experiments. (biomedcentral.com)
  • Over expression of p21 inhibited cell growth and concomitant treatment with Zyflamend enhanced this effect. (biomedcentral.com)
  • Our results suggest that the extracts of this polyherbal combination increase histone 3 acetylation, inhibit the expression of class I and class II histone deacetylases, increase the activation of CBP/p300 and inhibit cell proliferation, in part, by up regulating p21 expression. (biomedcentral.com)
  • Inactivation of p300 acetyltransferase reversed the resveratrol-induced expression of VEGFs and motor repair in rats that had undergone sciatic nerve crush injury. (vinomis.com)
  • This study used etoposide, to induce DNA damage-related senescence or chronic exposure to IL-1β to entail inflammation-related senescence in human OA chondrocytes. (aging-us.com)
  • Etoposide-induced senescence model may help investigate the initiation of cellular senescence in chondrocytes, and provide a useful model to develop therapeutic approaches to target senescence in OA. (aging-us.com)
  • Considering the role of cellular senescence in age-related diseases including OA, the therapeutic potential of senolytic (drugs that induced SnCs death) and senomorphic (drugs that modulate the SASP) compounds have been contemplated with growing interest [ 15 ]. (aging-us.com)
  • Importantly, mitotic slippage and oncogene signalling synergistically induced senescence and key senescence regulators p21 and p16. (nih.gov)
  • Results: We found that TGF beta similarly induced morphological changes regardless of genotype and independently of proliferation index or sensitivity to inhibition of proliferation by TGF beta. (st-andrews.ac.uk)
  • Ample evidence shows that pro-atherosclerotic disturbed flow induces sustained activation of atherogenic genes in endothelial cells to promote their oxidation, inflammation, cell cycle progression and proliferation, whereas pulsatile shear stress tends to maintain endothelial cells in a quiescent and less proliferative state with a low level of oxidation and inflammation [16] , [17] . (plos.org)
  • It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
  • Knockdown of p21 with siRNA technology attenuated Zyflamend-induced growth inhibition. (biomedcentral.com)
  • Passaging 40.0 times resulted in senescent fibroblasts with no growth over 14 days, morphological alterations typical of aged cells, and a rise in cell-cycle regulator p21 (CDKN1A) transcripts. (news-medical.net)
  • Polymorphism at codon 31 of CDKN1A (p21) as a predictive factor for bevacizumab therapy in glioblastoma multiforme. (cdc.gov)
  • Analysis of cTnI phosphorylation indicated that compared to WT, ISO-induced phosphorylation of cTnI was blunted in Pak-1-KO mice. (ox.ac.uk)
  • Src-induced phosphorylation of caveolin-2 on tyrosine 19. (wikipedia.org)
  • Also acts as a potent inhibitor of agonist-induced Ca 2+ sensitization of myosin phosphorylation and smooth muscle contractions (IC 50 = 700 nM for phenylephrine-induced contractions in aortic strips). (sigmaaldrich.com)
  • Also acts as a potent inhibitor of agonist-induced Ca 2+ sensitization of myosin phosphorylation and smooth muscle contraction. (sigmaaldrich.com)
  • Reactive oxygen species promote TNF α -induced death and sustained JNK activation by inhibiting MAP kinase phosphatases. (nature.com)
  • The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. (wikipedia.org)
  • The method could detect the consequences of a genetically induced epigenetic reversal of age using lentivirus transduction and gene ontology (GO) analysis. (news-medical.net)
  • Gene set enrichment analysis (GSEA) was performed to identify pathways related to the differences and similarities between chemical therapies, aging signatures, and the OSK(M)-induced iPSCs using the Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome pathways, and HALLMARK genomic databases. (news-medical.net)
  • Alu-repeat-induced deletions within the NCF2 gene causing p67-phox-deficient chronic granulomatous disease (CGD). (lu.se)
  • In order to understand the role of this unexpected regulatory event, we ablated the NFκB binding site we identified in the p21 promoter, using CRISPR/Cas9-mediated genome editing. (psu.edu)
  • The minimal p21 promoter region required for perifosine-induced p21 promoter activation contains four consensus Sp1-binding sites. (conicet.gov.ar)
  • En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
  • Our objective was to test the hypothesis that Pak-1/PP2A is a signaling cascade controlling stress-induced cardiac growth. (ox.ac.uk)
  • Atherosclerosis plaques preferentially develop at arterial branches and curvatures under low and oscillatory or even static shear stress induced by disturbed blood flow as opposed to in straight parts, featuring protective unidirectional pulsatile shear stress [14] , [15] . (plos.org)
  • Together our results demonstrate a new mechanism explaining hypoxia-induced resistance involving the alteration of the response to DSB in D283-MED cells, but also highlight the cell type to cell type diversity and the necessity to take into account the differing tumour genetic make-up when considering re-sensitisation therapeutic protocols. (biomedcentral.com)
  • ISO-induced cardiac hypertrophy and Erk1/2 activation in Pak-1-KO/ISO were attenuated when the selective Erk1/2 inhibitor FR180204 was administered. (ox.ac.uk)
  • Bone marrow stem cells are targets for benzene-induced cytotoxicity and DNA damage that could result in changes to the genome of these progenitor cells, thereby leading to hematopoietic disorders and cancers. (rti.org)
  • Interferons assist the immune response by inhibiting viral replication within host cells, activating natural killer cells, increasing antigen presentation to lymphocytes, and inducing the resistance of host cells to viral infection. (wikidoc.org)
  • In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. (novusbio.com)
  • One of the treatments applied in cancer is radiotherapy (RT), a therapeutic modality that uses ionizing radiation to induce damage in unwanted cells. (frontiersin.org)
  • The medical journal, Blood, recently published that Ivermectin induces chloride-dependent membrane hyperpolarization and cell death in leukemia cells (Blood, November 4, 2010, vol.115). (thehealthyplanet.com)
  • The paper states Ivermectin synergizes with chemo agents cytarabine and daunorubicin to induce cell death in leukemia cells. (thehealthyplanet.com)
  • The team compiled a list of compounds that effectively converted human and mouse somatic cells into chemically induced pluripotent stem cells (CiPSCs) and assessed them using the NCC assay. (news-medical.net)
  • 2006). The E3 Ubiquitin Ligase Itch Couples JNK Activation to TNF α -induced Cell Death by Inducing c-FLIP(L) Turnover. (nature.com)
  • In D283-MED and U87MG, chronic hypoxia (5 days), but not acute hypoxia (24 h) induced resistance to chemotherapy and X-ray irradiation. (biomedcentral.com)
  • Daily topical treatment with CWO induced dramatic regression of pre-malignant skin tumors and a two-fold reduction in cutaneous squamous cell carcinomas. (biorxiv.org)
  • Disturbed interaction of p21-rac with mutated p67-phox causes chronic granulomatous disease. (lu.se)
  • This weakening has been proposed to represent long-term depression (LTD) induced by sensory deprivation in vivo . (jneurosci.org)
  • suggesting that it represents LTD induced in vivo . (jneurosci.org)
  • For instance, mice are able to reconstitute most lymphomas in monkeys and humans woodchuck hepatitis virus induces major components of the human provides strong support for a direct hepatocellular carcinoma (HCC) haematolymphoid system including oncogenic role of EBV in vivo. (who.int)
  • Loss of sensitivity to p21 cell cycle control may be associated with prostatic cancer behavior. (scirp.org)
  • The data reported here represent the first in vitro demonstration of the effect of waterpipe smoke on cellular parameters providing evidence of the potential involvement of WPS in the pathogenesis of COPD through impairing cellular growth and inducing inflammation. (nih.gov)
  • Chemically induced reprogramming to reverse cellular aging. (news-medical.net)
  • This indicates that possible association of these genes with metastasis potential would be unlikely to involve TGF beta-induced EMT. (st-andrews.ac.uk)
  • The authors of the present study previously demonstrated that ectopically induced transcription factors, Octamer-binding transcription factor 4 (OCT-4), sex-determining region Y-box 2 (SOX2), and Kruppel-like factor 4 ( KLF-4) (collectively known as OSK factors) among mammals can reverse aging by restoring youthful patterns of deoxyribonucleic acid (DNA) methylation, transcriptomic profiles, and tissue functioning without cell identity loss. (news-medical.net)
  • Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
  • The inhibitory effects of the doenjang prepared with the grain-type meju using mixed starter cultures were investigated in azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colon carcinogenesis mice model. (carcinogenesis.com)
  • AOM and DSS colon carcinogenesis was induced in female C57BL/6 mice, and doenjang was orally administered for 4 weeks. (carcinogenesis.com)