Induce gene signaling pathway clone cells. Medical search. Frequent questions

LncRNA NALT interaction with NOTCH1 promoted cell proliferation in pediatric T cell acute lymphoblastic leukemia. (nih.gov)
It was also demonstrated that USF1 directly binds to the promoter region of 2,492 genes, which may be involved in the viral progression and cell proliferation pathways. (spandidos-publications.com)
When JNK signalling pathway is blocked by inhibitor SP600125, hepatic stellate cells (HSCs) activation is inhibited, with less proliferation and reduced expression of α-smooth muscle actin (α-SMA) [ 15 ]. (biomedcentral.com)
Downregulation of CDCA5 Can Inhibit Cell Proliferation, Migration, and Invasion, and Induce Apoptosis of Prostate Cancer Cells. (nih.gov)
Ly6C high MC presented downregulated co-stimulatory receptors (CD2, GITR, and TIM1) which direct immune cell proliferation, and upregulated co-stimulatory ligands (LIGHT and SEMA4A) which trigger antigen priming and differentiation. (frontiersin.org)
These factors stimulate proliferation, differentiation, and survival signals, as well as specialized functions in host resistance to pathogens. (sagepub.com)
Tyrosine 343 in the erythropoietin receptor positively regulates erythropoietin‐induced cell proliferation and Stat5 activation. (sagepub.com)
Here we identify CSNK1E , the gene encoding casein kinase 1 epsilon (CK1ε) as required specifically for the proliferation of breast cancer cells with activated β-catenin and confirm its role as a positive regulator of β-catenin-driven transcription. (plos.org)
The Wnt/β-catenin pathway plays a critical role in embryonic development, maintenance of multipotent progenitor cell populations and proliferation of many tissue types [1] , [2] . (plos.org)
Protein tyrosine kinase inhibitors inhibit both proliferation and chemotaxis of vascular smooth muscle cells. (researchmap.jp)
Bidirectional regulation of smooth muscle cell proliferation by IFN gamma. (researchmap.jp)
Protein kinase C-mediated inhibition of vascular smooth muscle cell proliferation : The isoform that may mediate G1/S inhibition. (researchmap.jp)
On the contrary, knockdown of Sprouty proteins increases proliferation of activated astrocytes and, consequently, reduces secondary brain damage in neuronal lesion models such as kainic acid-induced epilepsy or endothelin-induced ischemia. (springer.com)
Fig. 4and encodes a glutamate receptor involved JNJ-26481585 in growth proliferation and survival of glioma and melanoma cells (23). (molecularcircuit.com)
First, we found that GS-002 significantly inhibited cell proliferation and induced cell apoptosis in dose-dependent manners. (hindawi.com)
ATF-3 induces DNA synthesis and expression of cyclin D1 in hepatocytes [ 21 ] and is involved in serum-induced cell proliferation as a target gene of c-myc [ 10 ]. (hindawi.com)
We previously identified Macrophage Migration Inhibitory Factor (MIF) as a novel factor that can support the proliferation and/or survival of NSPCs based on in vitro functional cloning strategy and revealed that MIF can support the proliferation of human brain tumor-initiating cells (BTICs). (elsevierpure.com)
Using gene manipulation (over or downregulation of TPT1) techniques including CRISPR/Cas9-mediated heterozygous gene disruption showed that TPT1 contributed to the regulation of cell proliferation/survival in mouse NSPCs, human embryonic stem cell (hESC) derived-NSPCs, human-induced pluripotent stem cells (hiPSCs) derived-NSPCs and BTICs. (elsevierpure.com)
We also identified the MIF-CHD7-TPT1-SMO signaling axis in regulating hESC-NSPCs and BTICs proliferation. (elsevierpure.com)
B ) Morphology of induced pluripotent stem cells (iPSC) colonies, ( C ) proliferation rate of iPSCs, and ( D ) photoreceptor primary cilium of organoids were compared between WT and rd16 . (elifesciences.org)
The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. (origene.com)
Effects of chloroplast DNA content on the cell proliferation and aging in Chlamydomonas reinhardtii. (bio.net)
In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)
Proliferation of KRT14 -expressing basal cells leads to development of dermatoglyphics during the first trimester of gestation. (medscape.com)

Cell Death and Differentiation. (wikipedia.org)
These data demonstrate that Akt2 expression is activated during cellular differentiation and suggest that it functions in the signaling pathways of some adult tissues. (nih.gov)
We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)
We found a significantly lower expression of key autophagy- (ATG-) related genes in primary AML as compared to healthy granulocytes, an increased autophagic activity during all- trans retinoic acid- (ATRA-) induced neutrophil differentiation, and an impaired AML differentiation upon inhibition of ATG3, ATG4D, and ATG5. (hindawi.com)
Our data clearly suggest that granulocytic AML differentiation relies on noncanonical autophagy pathways and that restoring autophagic activity might be beneficial in differentiation therapies. (hindawi.com)
This study aims to explore molecule signaling mediating MC subset differentiation in HHcy and control mice. (frontiersin.org)
These changes in gene expression define a new differentiation pathway by which C. albicans adapts to the vascular compartment of its host. (nih.gov)
However, the use of bFGF for human ES cell culture, particularly at the high levels used by some for 'feeder-free' culture, must be considered carefully, as bFGF may drive ES cells towards differentiation. (ddw-online.com)
The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)
It was proposed by Conrad Hal Waddington to illustrate the various developmental pathways a cell might take toward differentiation. (asu.edu)
The epigenetic landscape integrates the connected concepts of competence, induction, and regulative abilities of the genes into a single model designed to explain cellular differentiation, a long standing problem in embryology. (asu.edu)
Furthermore, gene silencing of TPT1 caused defects in neuronal differentiation in the NSPCs in vitro. (elsevierpure.com)
A ) Morphology of Nrl -GFP wild-type (WT) and rd16 retinal organoids differentiated from mouse-induced pluripotent stem cells (iPSC) at various differentiation time points. (elifesciences.org)
Upon virus illness the IFN induction pathway is definitely induced by dsRNA molecules (intermediates of viral replication) that can be sensed by cellular retinoic acid-inducible gene I product (RIG-I)-like helicases (RIG-I and melanoma differentiation-associated protein 5 [MDA5]) (20). (biomasswars.com)
this finding indicates that the gene for NFJ syndrome is not located in the epidermal differentiation complex, the type II keratin cluster, or the desmosomal cadherin cluster, respectively. (medscape.com)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)

Loss of cell division cycle‑associated 5 promotes cell apoptosis by activating DNA damage response in clear cell renal cell carcinoma. (nih.gov)
Silencing oncogene cell division cycle associated 5 induces apoptosis and G1 phase arrest of non-small cell lung cancer cells via p53-p21 signaling pathway. (nih.gov)
The 'cancer platform' concept proposes that most oncogenic molecules also have the inherent ability to activate tumor suppressor genes or pathways through oncogene-induced apoptosis or senescence, thus limiting their oncogenic effects in a homeostatic fashion. (biomedcentral.com)
2) We investigate induction of apoptosis in breast cancer cells by TRAIL receptor agonists. (cancer.gov)
Cancer cells avoid apoptosis by a variety of genetic and epigenetic mechanisms. (cancer.gov)
In a second project we are investigating the induction of apoptosis by activation of death receptors for the ligand TRAIL in breast and ovarian cancer cells. (cancer.gov)
Our goal is to selectively trigger apoptosis in the cancer cells. (cancer.gov)
We have shown that most breast and ovarian cancer cell lines are resistant to the induction of apoptosis by TRAIL, the ligand for the death receptors DR4 and DR5. (cancer.gov)
We have demonstrated that resistance to TRAIL-induced apoptosis can be overcome by co-incubation of the cells with chemotherapeutic agents, semi-synthetic retinoids (such as 4HPR), or molecularly targeted agents (such as anti-ErbB-2 antibodies). (cancer.gov)
Autocrine FGF-2 is responsible for the cell density-dependent susceptibility to apoptosis of HUVEC : a role of a calpain inhibitor-sensitive mechanism. (researchmap.jp)
Oxidized low density lipoprotein induces apoptosis in cultured human umbilicalvein endothelial cells by common and unique mechanisms. (researchmap.jp)
Activating transcription factor-(ATF-) 3, a stress-inducible transcription factor, is rapidly upregulated under various stress conditions and plays an important role in inducing cancer cell apoptosis. (hindawi.com)
Furthermore, we found that GS-002 induced more cell apoptosis in ATF-3-overexpressing cells. (hindawi.com)
These results suggest that the induction of apoptosis by the propolis derivative, GS-002, is partially mediated through ER stress and ATF-3-dependent pathways, and GS-002 has the potential for development as an antitumor drug. (hindawi.com)
Recent research demonstrated that the potential to induce apoptosis through endoplasmic reticular (ER) stresses can be a target for cancer therapy. (hindawi.com)
Apoptosis is induced through the mitochondrial pathway resulting in caspase-9 activation and downstream caspase-3 activation. (cdc.gov)
Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. (cdc.gov)
We observed that the WNV-Cp protein is a pathogenic protein, which drives apoptosis in vitro through the mitochodrial/caspase-9 pathway. (cdc.gov)
We also observed that expression of Cp protein in mouse muscle resulted in apoptosis and inflammation of muscle cells. (cdc.gov)
In vitro apoptosis in individual cells was determined by terminal desoxyriboxyl-desoxyriboxyl transferase-mediated DVTP nick-end labeling (TUNEL) assay with the In Situ Cell Death Assay Kit (Roche Diagnostic Corp., Indianapolis, IN) and visualized by fluorescent microscopy. (cdc.gov)

The protein encoded by this gene shares significant sequence identity with the murine TSC-22 and Drosophila shs, both of which are leucine zipper proteins, that function as transcriptional regulators. (wikipedia.org)
1) We study Cbl proteins, a family of ubiquitin ligases that negatively regulate signaling by receptor tyrosine kinases. (cancer.gov)
We have shown that all mammalian Cbl proteins mediate ubiquitination and degradation of the activated EGFR as well as other components of the signaling complex. (cancer.gov)
Ongoing work is focused on understanding the biochemical and physiologic functions of the three mammalian Cbl proteins in epithelial cells and elucidating the differences in their specificity and/or function. (cancer.gov)
Use of feeder layers requires two cell types to be maintained in parallel and introduces mouse proteins into the culture system. (ddw-online.com)
In addition to ligand-regulated degradation of β-catenin, Wnt signaling is antagonized by extracellular factors that inhibit the ability of Wnt ligands to bind to Fzd and initiate signaling, such as the secreted frizzled-related proteins (SFRP1, WNT inhibitory factor (WIF) and dickkopf (DKK) [3] . (plos.org)
Among them are the Sprouty proteins which mainly act as inhibitors of growth factor-dependent neuronal and glial signaling pathways. (springer.com)
Most plasma membrane proteins are sorted by the trans -Golgi network (TGN) in the conventional secretory pathway. (jneurosci.org)
Whole cell lysates were probed for NF-κB and IκB family proteins by immunoblot. (biomedcentral.com)
GS-002 also induced endoplasmic reticular (ER) stress as evidenced by increases in ER stress-responsive proteins including glucose-regulated protein 78 (GRP78), growth arrest- and DNA damage-inducible gene 153 (GADD153), phosphorylated eukaryotic initiation factor 2 α (eIF2 α ), phosphorylated protein endoplasmic-reticular-resident kinase (PERK), and ATF-3. (hindawi.com)
He mentioned this to David Stillman , who was at Stanford to interview for a faculty position, and who studied cell cycle regulation of proteins as a postdoc in Kim Nasmyth 's lab at the MRC Laboratory of Molecular Biology in the U.K. Stillman pointed out that ribonucleotide reductase was cell cycle regulated-rather than remaining stable, the RNA and protein levels fluctuate throughout the cell cycle. (the-scientist.com)
Fig 1 Manifestation and subcellular localization of MERS-CoV accessory proteins in HEK-293T cells. (biomasswars.com)
A) The different accessory proteins 3 4 4 and 5 were indicated in HEK-293T cells fixed after 24 h 1-NA-PP1 and stained having a mouse anti-FLAG immunoglobulin G (IgG) … After confirmation of protein expression we investigated putative IFN antagonistic functions. (biomasswars.com)
135 antibodies against 65 mainly immunoregulatory proteins, we of a multitude of different genes (8, 9). (lu.se)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)

Molecular cloning of L-JAK, a Janus family protein-tyrosine kinase expressed in natural killer cells and activated leukocytes. (sagepub.com)
The molecular basis of X-linked severe combined immunodeficiency: defective cytokine receptor signaling. (sagepub.com)
Ten JNK isoforms were identified in human brain by molecular cloning. (umassmed.edu)
We forecast that clone-specific practical profiling of GBMs can help determine intense clones new tumor driver systems molecular signatures and restorative vulnerabilities emphasizing the potential of tumor treatment at a clone-specific level. (molecularcircuit.com)
One of the key areas in stem cell research is the identification of factors capable of promoting the expansion of Neural Stem Cell/Progenitor Cells (NSPCs) and understanding their molecular mechanisms for future use in clinical settings. (elsevierpure.com)
but the molecular mechanisms underlying how cells adapt to variations in oxygen supply were unknown until the prize-winning work described here. (nobelprize.org)
While most molecular biologists thought signaling pathways worked by sensing signals extrinsic to the cell and relaying the information to the nucleus, Elledge was proposing an internal signaling pathway that senses cell-intrinsic events. (the-scientist.com)
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)
Molecular characterization and gene expression of lhcb5 gene encoding CP26 in the light-harvesting complex II of Chlamydomonas reinhardtii. (bio.net)
Cell culture systems contribute to identifying molecular mechanisms for HBV and HDV propagation, finding drug targets for antiviral therapies, and testing antiviral agents. (cdc.gov)
When the iv mouse mutation was cloned, it was found to encode a molecular motor protein, an axonemal dynein, and was named lrd , for left-right dynein (human homolog is DNAH11 / DNAHC11 ). (medscape.com)
Correspondence with low molecular weight chemicals that results in the priming of chemical-specific, Prof. Dr. Stefan F. Martin, Allergy Research skin-homing CD8+ Tc1/Tc17 and CD4+ Th1/Th17 cells. (cdc.gov)
DAMPs, damage-associated tissues and the molecular target structures and pathways. (cdc.gov)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)

Huh7 cells that overexpress USF1 were used with whole transcriptome profiling through RNA sequencing and chromatin immunoprecipitation (ChIP) sequencing methods to investigate the downstream targets of USF1. (spandidos-publications.com)
Downstream genes, including lncRNA‑NEAT1 and TF‑ETV5, may also have potential functions in the regulated network by USF1 and have potential functions in the progression of HCC. (spandidos-publications.com)
The major focus of the Roberts lab is to define functions of the extracellular matrix protein thrombospondin-1, its receptors, and downstream signal transduction pathways in cancer. (nih.gov)
In recent years, the search for intracellular signaling integrator downstream of receptor tyrosine kinases provided valuable novel substrates. (springer.com)
However, the detailed downstream signaling for the functions has largely remained unknown. (elsevierpure.com)
Thus, in the present study, we newly identified translationally-controlled tumor protein-1 (TPT1), which is expressed in the ventricular zone of mouse embryonic brain, as a downstream target of MIF signaling in mouse and human NSPCs and human BTICs. (elsevierpure.com)
Downstream signaling entails activation of IFN regulatory element 3 (IRF-3). (biomasswars.com)

Phospholipase A2 is well known for its role in liver fibrosis and inhibition of tumour cells. (biomedcentral.com)
utilizes a combination of high-resolution comparative genomic hybridization, short hairpin RNA inhibition of target genes at the locations of focal genomic deletions, and a primed cell mosaic mouse model to identify novel tumor suppressors in hepatocellular carcinoma. (biomedcentral.com)
Oncogene activation is therefore generally only tumorigenic when it is coupled with inactivation or inhibition of oncogene-induced pro-apoptotic or senescence pathways [ 4 ]. (biomedcentral.com)
An advantage of targeting MEK is that the Ras / Raf / MEK / ERK a focal point, where a number of upstream signaling pathways through inhibition of MEK can be blocked. (nartsignaling.com)
Protein kinase C-mediated inhibition of cyclin A expression in human vascular endothelial cells. (researchmap.jp)
Using the bioinformatics tool MAGICTRICKS and reversible pharmacological inhibition, we found that primed genes required the transcriptional activities of NF-κB. (frontiersin.org)

this included the TNF‑α pathway, the NF‑κB pathway, the MAPK signaling pathway, the Notch signaling pathway and Axon guidance. (spandidos-publications.com)
We have found that, although chronic TNF attenuates TCRζ expression and TCR-proximal signalling, IL-2 expression is suppressed regardless of whether cells are stimulated via the TCR, or with phorbol ester and calcium ionophore to activate the MAPK and NFAT pathways directly. (biomedcentral.com)
The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)

In vitro, HSCs were incubated with MBP- Cs sPLA2 to detect the activation of HSCs as well as the activation of JNK signalling pathway. (biomedcentral.com)
In vitro , MBP- Cs sPLA2, as well as the mutant, was incubated with HSCs and it was proved that activation of HSCs was related to activation of the JNK signalling pathway instead of the enzymatic activity of MBP- Cs sPLA2. (biomedcentral.com)
We identified a hemoglobin-induced cell wall protein that binds to fibronectin and cloned several novel genes that are rapidly induced in cells exposed to hemoglobin in vitro or by incubation in the vascular compartment of rabbits. (nih.gov)
ONC201 kills breast cancer cells in vitro by targeting mitochondria. (cancer.gov)
Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)
In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro . (hindawi.com)
In vitro cell culture systems are instrumental in the development of therapeutic targets. (cdc.gov)
A robust in vitro cell culture system supporting HBV and HDV infections (HBV/HDV) is a critical prerequisite to studying HBV/HDV pathogenesis, the complete life cycle of HBV/HDV infections, and consequently identifying new therapeutics. (cdc.gov)
In vitro cell culture models have evolved with significant improvements over several decades. (cdc.gov)

The expression of this gene is stimulated by glucocorticoids and interleukin 10, and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid and chemokine. (wikipedia.org)
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. (wikipedia.org)
Akt2 expression was low in a multipotent fibroblast cell line, but it was upregulated when these cells were transformed with Myod and induced to differentiate into myocytes. (nih.gov)
Promoter region motif analysis indicated that ETV5 has more binding motifs and genes than USF1 itself for USF1‑regulated DEGs, indicating that USF1 may indirectly modulate gene expression by regulating ETV5 expression in Huh7 cells. (spandidos-publications.com)
As a TF, USF1 has a bidirectional regulatory function, being able to regulate gene expression by activating or suppressing the promoter region of target genes ( 5 , 6 ). (spandidos-publications.com)
Flow cytometric analysis provided essential information of the cell line HLA-1 expression. (helsinki.fi)
Additional information of PD-L1 expression can be used to evaluate cell line's immune-evasion abilities. (helsinki.fi)
Low PU.1 expression in AML may account for low ATG gene expression in this disease. (hindawi.com)
In addition, ribosomal genes exhibit specific expression patterns depending on the recombinant protein and the production rate. (bvsalud.org)
In a clone displaying a dramatically increased EPO secretion, we detect higher gene expression related to negative regulation of endoplasmic reticulum (ER) stress, including upregulation of ATF6B, which aids EPO production in a subset of clones by overexpression or small interfering RNA (siRNA) knockdown. (bvsalud.org)
Human MC were initially divided into three subsets based on the cell surface expression of CD14 and CD16, and recently classified based on CD40 expression ( 2 - 5 ). (frontiersin.org)
Increased expression of inflammatory cytokines, lysyl oxidase, transforming growth factor-β, impaired megakaryocyte function, and aberrant JAK-STAT signaling have all been implicated in the pathogenesis of bone marrow fibrosis. (haematologica.org)
However, better understanding of the role of increased JAK-STAT signaling [either through activating mutations ( JAK2 , MPL515L/K ) within the signaling pathway, or mutations involving CALR ], the role of deregulated pro-inflammatory cytokine expression, and the impaired bone marrow microenvironment is transforming the treatment approach for MF. (haematologica.org)
Our mechanistic studies established that CD47 blockade directly enhances survival of nontransformed cells under stress by stimulating a protective autophagy response and by inducing expression of c-Myc and several additional transcription factors that promote stem cell self-renewal. (nih.gov)
We found that hemoglobin specifically induces expression of a C. albicans receptor for the host matrix protein fibronectin. (nih.gov)
STATs then can modulate the expression of target genes. (sagepub.com)
Heterogeneous antigen expression is a key barrier influencing the activity of chimeric antigen receptor (CAR) T cells in solid tumors. (nature.com)
CAR T-cell potency is highly dependent on target antigen expression, and CARs often fail to exert their antitumor activities when antigen expression is low or below a certain threshold. (nature.com)
This thesis aimed to 1) lower the cost of production by using cheap C1 carbon sources (e.g. methane) from agricultural activities (e.g. intensive dairy, piggeries, etc.) for the cultivation of transformed yeast and 2) to assess the therapeutic efficacy of recombinant hIFNγ in its glycosylated and non-glycosylated form from different expression systems against ovarian cancer cells. (edu.au)
In the fouth chapter, the aim was to study how selective pressure on the Histidinol dehydrogenase gene (HIS4), using amino acid starvation, affects the level of expression and secretion of the adjacent hIFNγ in the transformed P. pastoris GS115 strain, a histidine-deficient mutant. (edu.au)
As shown by ELISA, amino acid starvation-induced selective pressure on HIS4 improved expression and secretion of the adjacent hIFNγ by 55% compared to unchallenged cells. (edu.au)
Results from RNA sequencing revealed that AEG‑1 modulated neuronal morphology and development by regulating the expression of numerous genes, such as ubiquitin C, C‑X‑C motif chemokine ligand 1, MMP9, Notch1, neuropilin 1 and ephrin type‑A receptor 4. (spandidos-publications.com)
In addition, upregulation of AEG-1 expression can protect nigral dopaminergic neurons from injury caused by aberrant apoptotic signaling pathways ( 13 ). (spandidos-publications.com)
GBP1 expression is induced by Interferon g as well as other inflammatory cytokines including IL-1a, IL-1b and TNF-a. (thermofisher.com)
Induction of GBP1 expression by poly I:C, a prototypical TLR3 ligand, as well as GBP1 expression dependency on NF-kB and IFNa response elements implicate TLR pathway involvement. (thermofisher.com)
Inversely proportional expression of GBP1 and MMP-1 as well as other related molecules to adhesion and migration show a clear functional role of GBP1 in suppressing angiogenesis and migration especially of endothelial cells. (thermofisher.com)
We also find that expression of CK1ε is able to promote oncogenic transformation of human cells in a β-catenin-dependent manner. (plos.org)
Methylation of mammalian DNA and histone residues are known to regulate transcription, and the discovery of demethylases that remove methylation in DNA and histones provide a basis for the understanding of dynamic regulation of mammalian gene expression. (cmbn.no)
Changes of gene expression by lysophosphatidylcholine in vascular endothelial cells, 12 upregulated distinct genes including 5 cell growth-related, 3 thrombosis-related. (researchmap.jp)
Protein tyrosines and protein kinase C are required for gene expression of monocyte chemoattractant protein-1 in human monocytes. (researchmap.jp)
Prostaglandin A and J arrest the cell cycle of cultured vascular smooth muscle cells without suppression of c-myc expression. (researchmap.jp)
We, therefore, tested the hypothesis that hypoxia would enhance or "prime" microglial pro-inflammatory gene expression in response to a later inflammatory challenge without programmatically increasing basal levels of pro-inflammatory cytokine expression. (frontiersin.org)
We used RNA sequencing coupled with chromatin immunoprecipitation sequencing to analyze primed microglial inflammatory gene expression and modifications to histone H3 lysine 4 trimethylation (H3K4me3) at the promoters of primed genes. (frontiersin.org)
However, interfering with Sprouty expression by gene therapy or siRNA treatment provides a realistic approach to evaluate the therapeutic potential of indirectly stimulating ERK activities in neurological disease. (springer.com)
Chronic high glucose dramatically influences β-cell lipid metabolism via substrate availability, changes in the activity and expression of enzymes of glucose and lipid metabolism, and modifications in the expression level of key transcription factors. (diabetesjournals.org)
For example the well-defined GBM oncogenes hepatocyte growth factor receptor (MET) and show reduced expression in the TMZ-sensitive clone of GBM-482. (molecularcircuit.com)
and have been shown to induce vasculature JNJ-26481585 in the central nervous system (26 27 Interestingly the TMZ-sensitive clone shows increased expression of several genes involved JNJ-26481585 in neurotransmitter signaling such as glutamate receptors (did not correlate with TMZ responsiveness suggesting that new biomarkers of drug responsiveness are sorely needed consistent with more recent bulk GBM genomic analyses which highlight the subgroup limitations of this marker (1). (molecularcircuit.com)
How does tumour necrosis factor uncouple T-cell receptor-induced IL-2 gene expression in murine T cells? (biomedcentral.com)
T-cell receptor (TCR)-induced IL-2 expression is suppressed at the level of mRNA and protein following chronic culture of T cells with tumour necrosis factor (TNF) at picomolar concentrations, an effect that is reversible upon removal of TNF. (biomedcentral.com)
However, the initial rate of decay was similar in control and TNF-treated cells, suggesting that reduced expression in TNF-treated cells was not due to decreased stability of IL-2 mRNA. (biomedcentral.com)
c-Rel, IκBβ and IκBε were regulated differently in control and TNF-treated cells: expression of IκBε was comparatively enhanced, while that of c-Rel and IκBβ was attenuated in TNF-treated cells. (biomedcentral.com)
However, altered regulation of NF-κB expression and activity in TNF-treated cells may contribute to poor inducibility of IL-2 through effects on stimulus-induced changes in chromatin conformation across pIL-2. (biomedcentral.com)
Isomäki P, Panesar M, Annenkov A, Clark JM, Foxwell BM, Chernajovsky Y, Cope AP: Prolonged exposure of T cells to TNF down-regulates TCR zeta and expression of the TCR/CD3 complex at the cell surface. (biomedcentral.com)
Animal cells undergo fundamental shifts in gene expression when there are changes in the oxygen levels around them. (nobelprize.org)
These changes in gene expression alter cell metabolism, tissue re-modeling, and even organismal responses such as increases in heart rate and ventilation. (nobelprize.org)
Through the combined work of these three laureates it was thus demonstrated that the response by gene expression to changes in oxygen is directly coupled to oxygen levels in the animal cell, allowing immediate cellular responses to occur to oxygenation through the action of the HIF transcription factor. (nobelprize.org)
However, for most of the 20th century, it was not clear how adaptations to oxygen flux were regulated at the fundamental level of gene expression. (nobelprize.org)
This clone was engineered to express the complete ORF with an expression tag. (origene.com)
Expression varies depending on the nature of the gene. (origene.com)
For protein expression human being embryonic kidney HEK-293T cells were transfected with FuGENE HD (Promega). (biomasswars.com)
The localization pattern of capsid expression was analyzed by immunofluorescent assay in HeLa, 293-T, RD, or SH-SY5Y cells by using anti-His tag antibody as described ( 6 ). (cdc.gov)
Furthermore, we analyzed differential gene expression profiles related to each vaccine delivery group and found that tissue stress, T cell receptor signaling, and NFB signaling pathways were activated in all groups. (cdc.gov)
3. Translational research - to identify predictive biomarkers for treatment decision - by immunohistochemistry, gene expression or sequencing. (lu.se)

The specific effects that lead to inactivation of these pro-apoptotic or senescence pathways may impinge on major known tumor suppressors such as p53, but may also be due to effects on other genes and molecules within the p53 or other tumor suppressor pathways [ 5 ]. (biomedcentral.com)
The basic biology of this system, including the range of known Jaks and STATs, is discussed, as are the defects in animals and humans lacking some of these signaling molecules. (sagepub.com)
Endocytosis is a fundamental process involved in trafficking of various extracellular and transmembrane molecules from the cell surface to its interior. (mdpi.com)
Mutagenesis signalling molecules cade. (who.int)
MW) chemicals interact with host cells and molecules and In our daily life, we are confronted with a plethora of natural influence biologic processes such as signal transduction. (cdc.gov)
Our facilities provide the opportunity to study molecules, cells, organs and entire organisms. (lu.se)

The NO/cGMP cascade is only one of several signaling pathways that mediate the pro-angiogenic activities of VEGF, so we now recognize that the anti-angiogenic activities of thrombospondin-1/CD47 signaling extend beyond the NO/cGMP cascade. (nih.gov)

METHODS: Whole-cell patch-clamp experiments were performed in human embryonic kidney (HEK 293) cells and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) together with immunofluorescent confocal and high resolution microscopy, auto-ubiquitinylation assays, and co-immunoprecipitation experiments to test the functional interactions between hERG and RNF207. (bvsalud.org)
Targeted disruption of the mouse Stat3 gene leads to early embryonic lethality. (sagepub.com)
The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. (thermofisher.com)
Stem cells, and in particular human embryonic stem (ES) cells, have earned a reputation for being labour intensive and difficult to grow and control in culture. (ddw-online.com)
Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)

Monocytes (MC) are bone marrow (BM) derived mononuclear phagocytes that play an important role in innate immune response and are the major immune cell population in chronic tissue inflammatory ( 1 , 2 ). (frontiersin.org)
This demonstrates the intersection of two key pathways of innate immune regulation, NLR and STING, to fine tune host response to intracellular DNA, DNA virus, and c-di-GMP. (umassmed.edu)
These results suggest that HBsAg delivered by dMNP, IM AFV, and IM AAV have similar signaling pathways to induce innate and adaptive immune responses. (cdc.gov)
To cite this article: Martin SF, Esser PR, Weber FC, Jakob T, Freudenberg MA, Schmidt M, Goebeler M. Mechanisms of chemical-induced innate immunity in allergic contact dermatitis. (cdc.gov)
Contact allergen-induced innate immunity contrast to irritant contact dermatitis (ICD), which evolves emollients may help reduce irritancy and stabilize the barrier as a consequence of direct toxic effects of physical or chemi- function of the skin avoiding the penetration of contact aller- cal agents resulting in keratinocyte damage and local inflam- gens. (cdc.gov)

Although cytokines are known to activate multiple signaling pathways that together mediate these important functions, one of these pathways, the Jak-STAT pathway, is the focus of this chapter. (sagepub.com)

Once stabilized, β-catenin accumulates and translocates to the nucleus, where it complexes with TCF/LEF to activate transcription of target genes, such as MYC and CCND1 . (plos.org)

These studies have revealed multiple mechanisms and pathways in the carcinogenic process. (biomedcentral.com)

Finally, the presence of RNF207G603fs with hERGT613M results in significantly prolonged action potential durations and reduced hERG current in human-induced pluripotent stem cell-derived cardiomyocytes. (bvsalud.org)
Karyotypes of induced pluripotent stem cell lines. (elifesciences.org)
Phenotypes of retinal organoids differentiated from induced pluripotent stem cells of Nrl -GFP rd16 mice. (elifesciences.org)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)

The upregulated DEGs were primarily protein‑coding genes enriched in immune and inflammation response pathways, while the downregulated DEGs were mainly coding long non‑coding (lnc)RNAs, indicating the regulatory function of USF1. (spandidos-publications.com)
Mice primed with either ovalbumin or methylated BSA also showed enhanced antigen-specific immune response of both T and B cells, an immunological abnormality that exacerbated antigen-induced arthritis. (jci.org)
Even though the idea of an immune system actively searching for and disposing of potential mutated tumor cells is over a century old, only recent developments in various fields such as mass spectrometry, immuno-checkpoint blockade strategies and in silico modelling have enabled the realization of the full potential of recruiting immune system to fight cancer and the possibilities of personalized therapies. (helsinki.fi)
As described below, these diverse situations range from the complications of diabetes and cellular perturbation in amyloidoses to immune and inflammatory responses and tumor cell behavior. (jci.org)
In addition to immune defects that affect IgE synthesis, defects of cell-mediated immunity have also been reported, consistent with impaired TH 1 responses. (medscape.com)

Although bone marrow fibrosis is seen in a variety of malignant and non-malignant disease states, the deposition of reticulin and collagen fibrosis in the bone marrow of patients with myelofibrosis is believed to be mediated by the myelofibrosis hematopoietic stem/progenitor cell, contributing to an impaired microenvironment favoring malignant over normal hematopoiesis. (haematologica.org)
We discuss the rationale of various anti-fibrogenic treatment strategies targeting the clonal hematopoietic stem/progenitor cell, aberrant signaling pathways, fibrogenic cytokines, and the tumor microenvironment. (haematologica.org)
Use of progenitor cell lines can eliminate the need to culture challenging human ES cells. (ddw-online.com)

Initially focusing on the NO/cGMP pathway, we predicted that antagonists of thrombospondin-1/CD47 signaling would improve tissue survival of ischemic injuries by enhancing NO-dependent tissue perfusion. (nih.gov)
We report that the WNV-capsid (Cp) by itself induces rapid nuclear condensation and cell death in tissue culture. (cdc.gov)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)

Our previous study of the human ether-à-go-go related gene (hERG)-encoded K+ channel (Kv11.1) supports an association between hERG and RING finger protein 207 (RNF207) variants in aggravating the onset and severity of LQTS, specifically T613M hERG (hERGT613M) and RNF207 frameshift (RNF207G603fs) mutations. (bvsalud.org)
Loss-of-function mutations in APC or AXIN or activating mutations in the gene encoding β-catenin, CTNNB1 , lead to aberrant activation of Wnt/β-catenin signaling and have been causally linked to tumorigenesis of the colon, liver and skin [1] , [4] . (plos.org)
Although mutations in these same genes have not been observed as recurrent genetic events in breast tumors, there is strong evidence implicating Wnt/β-catenin activity in breast tumorigenesis. (plos.org)
Mutations of the signal transducer and activator of transcription 3 (STAT3) gene were shown to cause the AD HIES by 2 groups independently. (medscape.com)
9] Recent progress in immunological research continues to find other gene mutations that can manifest as overlapping clinical features of HIES (high IgE, allergic symptoms, vulnerability to fungal and bacterial infection). (medscape.com)
He found that RNR2 RNA levels increased dramatically, even more than the protein levels, upon exposure of cells to DNA damage and that mutations in RNR2 resulted in hypersensitivity to DNA damage. (the-scientist.com)
Activating mutations upstream may also underlie some epigenetic or within the ERK1/2 cascade are events that change cell signalling. (who.int)
If this is the case, gene-correction presents a solution for patients with known monogenetic mutations. (lu.se)

The ULK1 protein complex including ULK1, ATG13, and FIP200 coordinates the autophagy initiation from different upstream signaling pathways to induce autophagy [ 6 , 7 ]. (hindawi.com)
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1a induced cell death. (lu.se)

My laboratory studies signal transduction pathways that regulate growth and programmed cell death in epithelial cancer cells, with a focus on breast and ovarian cancer. (cancer.gov)

The presence of activated ras, which necessarily require for translocation from the cytoplasm to the Telaprevir VX-950 membrane and the arrangement in the signal complex This has therapeutic implications, as patients with mutant RAS, when they bind with specific inhibitors of Raf Raf BB treated Raf and activate a and f Rdern oncogenic pathway. (nartsignaling.com)

This pathway is triggered by both cytokines and interferons, and it very rapidly allows the transduction of an extracellular signal into the nucleus. (sagepub.com)
This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. (origene.com)

Several main apoptotic indicators were found in GS-002-treated cells, such as the cleaved forms of caspase-3, caspase-9, and poly(ADP-ribose) polymerase (PARP). (hindawi.com)
May participate in the GZMB apoptotic pathways. (lu.se)

In addition, AEG-1 as a key modulator regulates aberrant cellular processes within the central nervous system (CNS), where it is involved in neurological diseases such as Huntington's chorea, migraine and HIV-induced neurological disorders ( 7-9 ). (spandidos-publications.com)
Aberrant β-catenin signaling plays a key role in several cancer types, notably colon, liver and breast cancer. (plos.org)

Ly6C high MC exhibited activated neutrophil degranulation, lysosome, cytokine production/receptor interaction and myeloid cell activation pathways, and Ly6C low MC presented features of lymphocyte immunity pathways in both mice. (frontiersin.org)
Our current major objectives are 1) to identify mechanisms by which thrombospondin-1 signaling through its receptor CD47 regulates angiogenesis and tumor perfusion, 2) to define CD47 signaling pathways that differentially regulate self-renewal and autophagy in normal versus tumor cells, 3) to understand the mechanisms by which thrombospondin-1 regulates anti-tumor adaptive immunity, and 4) identifying genes required for disseminated infections by the pathogenic yeast Candida albicans . (nih.gov)

Significantly differentially expressed (SDE) immunological genes and transcription factor (TF) were selected for functional pathways and transcriptional signaling identification. (frontiersin.org)
Reverse transcription‑quantitative PCR and western blotting were carried out to analyze the knockdown efficiency of AEG‑1‑deficient HT22 cell line. (spandidos-publications.com)
In studies during the early 1990's, Gregg Semenza identified, and then in 1995 purified and cloned, a transcription factor that regulates these oxygen-dependent responses. (nobelprize.org)

In contrast to T cells, which did not exhibit a significant difference, B cells from PPARγ +/- showed an enhanced proliferative response to stimulation by either lipopolysaccharide or cross-linking of antigen receptors. (jci.org)
These therapeutic methods, including but not limited to oncolytic virus therapies, T-cell therapies and cancer vaccines, are based on the body's ability to recognize mutated antigen peptides presented on the cell surface by MCH-receptors (also known as HLA-receptors in humans) and the disposal of the malignant cells by cytotoxic T-cells. (helsinki.fi)
Our previous work established that several integrin and non-integrin receptors mediate the complex actions of thrombospondin-1 on vascular cells. (nih.gov)
As an alternative approach, growth factor receptors or components of their signal transduction machinery may be targeted directly. (springer.com)
e.g. inflammation, genotoxicity, and This chapter focuses on issues as- ellers, growth factors, growth factor epigenetic alterations) and can have sociated with the understanding and receptors, signal transducers, and both genetic and epigenetic origins. (who.int)

and regulation of cell cycle process. (nih.gov)
One of these genes, HBR1 , is essential for vegetative growth and is haploinsufficient for regulation of mating type locus genes, white-opaque switching, and some stress responses. (nih.gov)
Collectively, the present study provides insights into AEG‑1‑dependent gene regulation and signaling pathway transduction in neurons. (spandidos-publications.com)
Knowledge on these demethylases has led to a tremendous progress in the understanding of methyl marks in gene regulation and role in numerous diseases. (cmbn.no)

In order to overcome these limitations, this study explored the possibility to use cancer cell derived extracellular vesicles (EVs) as drug delivery system for oncolytic adenovirus. (helsinki.fi)
Alternatively, stem cell cultures can be grown on extracellular matrix extracts and supplemented with conditioned medium from mouse fibroblast cultures. (ddw-online.com)

These ATG genes are highly conserved in mammalian cells, allowing to study their functions also in higher eukaryotes [ 1 - 4 ]. (hindawi.com)

If unconventional secretion were to mediate the cilia trafficking of P/rds, then cooperation with the conventional secretory pathway that mediates the trafficking of rhodopsin would be required to ensure proper morphogenesis of the photoreceptive disk membranes. (jneurosci.org)
Studying RNR2' s regulatory elements, he found those that were necessary to induce the production of higher protein levels in response to DNA damage and identified factors that bind these DNA elements to mediate the response of RNR2 to DNA damage. (the-scientist.com)

The results showed that USF1 significantly regulates 350 differentially expressed genes (DEGs). (spandidos-publications.com)
Protein phosphorylation regulates actomyosin-driven vesicle movement in cell extracts isolated from the green algae, Chara corallina. (bio.net)

Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (nih.gov)
Twenty-four potential transcriptional regulatory pathways were identified based on SDE TFs matched with their corresponding SDE immunological genes. (frontiersin.org)

Cs sPLA2 of C. sinensis may lead to liver fibrosis and HSCs activation which is related to c-Jun N-terminal kinase (JNK) signalling pathway. (biomedcentral.com)
A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. (thermofisher.com)
TIE2, also known as TEK tyrosine kinase, TIE2 gene is mapped to 9p21.2. (thermofisher.com)
This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. (thermofisher.com)
Even dead BRAF kinase, which are observed in human cancers, mutant B-Raf Raf protein can dimerize with 1 when stimulated by the mutated Ras protein and release signaling cascade Raf / MEK / ERK. (nartsignaling.com)
Phosphatidylinositol 3-kinase is required for growth factor-induced amino acid uptake by vascular smooth muscle cells. (researchmap.jp)
Phosphatidylinositol 3-kinase-independent signal transduction pathway for PDGF-induced chemotaxis. (researchmap.jp)
Tyrosine kinase ingibitors inhibit multiple steps of the cell cycle of vascular smooth muscle cells. (researchmap.jp)
Stimulator of interferon genes (STING, also named MITA, MYPS, or ERIS) is an intracellular DNA sensor that induces type I interferon through its interaction with TANK-binding kinase 1 (TBK1). (umassmed.edu)
Previous studies reported that ATF-3 was induced by treating cells with antitumorigenic compounds [ 14 - 18 ] and a phosphoinositide 3-kinase inhibitor [ 19 ]. (hindawi.com)
The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation. (bio.net)

Astrocyte elevated gene‑1 (AEG‑1) is a key regulatory factor of progression in multiple types of tumor and neurodegenerative disease development. (spandidos-publications.com)
As a regulatory molecule it is centrally involved in signal transduction of inflammatory and cell migration networks. (thermofisher.com)
Treg, regulatory T cell. (cdc.gov)

Nonetheless, what has recently emerged is that insulin resistance alone (in the non-β-cell target tissues of the hormone) cannot cause diabetes without β-cell failure ( 10 ). (diabetesjournals.org)

The results suggest that exposure to GLA, even in low doses, during infantile period in the rat induces alterations in the kainic acid receptor in the brain. (go.jp)

In genotypic sex determination, also called genetic or chromosomal sex determination, an organism's genes determine which hormones are produced. (asu.edu)

Increased synthesis under pathological conditions makes Sprouty2 an attractive pharmacological target to enhance intracellular signaling activities, notably the ERK pathway, in affected neurons or activated astrocytes. (springer.com)
Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)

Applications Tested: The TEK4 antibody has been tested by blocking of staining with fluorochrome conjugated TEK4 on mouse bone marrow cells. (thermofisher.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
C ) Fluorescent images of dissociated day ( D ) 28 WT and rd16 cells stained by 4',6-diamidino-2-phenylindole (DAPI) and anti-GFP antibody. (elifesciences.org)
Vaccination by dMNP induced protective levels of anti-HBs antibody responses (10 mIU/ml) in mice and rhesus macaques at all three HBsAg doses studied. (cdc.gov)
HBsAg delivered by dMNP induced higher anti-HBsAg antibody (anti-HBs) responses than the 10g IM AFV, but lower responses than 10g IM AAV, in mice and rhesus macaques. (cdc.gov)
This study provides evidence that delivery of 10g (birth dose) AFV by dMNP induced protective levels of antibody responses in mice and rhesus macaques. (cdc.gov)

Thrombospondin-1 signaling through CD47 redundantly inhibits the nitric oxide (NO)/cGMP cascade in vascular endothelial and smooth muscle cells. (nih.gov)
Through this pathway thrombospondin-1/CD47 signaling simultaneously inhibits tumor angiogenesis and perfusion. (nih.gov)
In addition, we discovered that CD47 interacts directly with VEGF receptor-2 and inhibits its signaling. (nih.gov)

Mitogenic activity of interferon gamma on growth-arrested human vascular smooth muscle cells. (researchmap.jp)
Interferon gamma suppresses PDGF production from THP-1 cells and blood monocyte-derived macrophages. (researchmap.jp)

Both glucose and fatty acids may cause good/adaptive or evil/toxic actions on the β-cell, depending on their concentrations and the time during which they are elevated. (diabetesjournals.org)

The article shows that doses of anesthesia insufficient to affect neuronal responses to whisker stimulation suppressed astrocyte calcium signals. (cmbn.no)
We are studying the mechanisms by which chronic TNF suppresses T-cell responses in the CD4 + mouse T-cell hybridoma clone, 11A2. (biomedcentral.com)
These altered responses may affect the ability of TNF-treated cells to remodel pIL-2 productively through NFκB consensus binding upon cell stimulation [ 4 ]. (biomedcentral.com)
Clark JM, Annenkov AE, Panesar M, Isomäki P, Chernajovsky Y, Cope AP: T cell receptor zeta reconstitution fails to restore responses of T cells rendered hyporesponsive by tumor necrosis factor alpha. (biomedcentral.com)
HBsAg-specific CD4+ and CD8+ T cell responses were detected in all vaccine groups. (cdc.gov)

This master thesis is a part of a project aiming to set up immunoaffinity-purification/MS based method in order to analyse the ligandome and determine T-cell recognized cancer associated antigens from tumor cells. (helsinki.fi)

Direct neuronal conversion of resident glial cells is advantageous since they are ubiquitously distributed brain cells able to self-renew and replenish their number, making them ideal candidates for endogenous repair. (lu.se)

The JNK signalling pathway is involved in hepatic stellate cells (HSCs) activation. (biomedcentral.com)
Western blot of mouse liver was done to detect the activation of JNK signalling pathway. (biomedcentral.com)
Activation of HSCs by Cs sPLA2 is related to the activation of the JNK signalling pathway instead of the enzymatic activity of Cs sPLA2. (biomedcentral.com)
Dysregulation of the NF-κB pathway in B cells from PPARγ +/- was indicated by spontaneous NF-κB activation, as well as increased IκBα phosphorylation and gel-shift activity following LPS stimulation. (jci.org)
Binding of these ligands to RAGE does not accelerate clearance or degradation but rather begins a sustained period of cellular activation mediated by receptor-dependent signaling. (jci.org)
Treatment of cells with interleukin-1 (IL-1) caused activation of the JNK isoforms. (umassmed.edu)
Activation of MET enhances GBM cell migration (24) and tumor cell resistance in response to DNA damage (25). (molecularcircuit.com)
In breast cancer, ATF-3 enhances cancer cell-initiating features [ 22 ] and is associated with activation of the canonical Wnt/ β -catenin pathway [ 23 ]. (hindawi.com)
The resulting aggregate called death-inducing signaling complex (disc) performs CASP8 proteolytic activation. (lu.se)

Promoters of genes associated with oxidative phosphorylation are preferentially remethylated at the 8-cell stage, suggesting that this mode of energy metabolism may not be favored. (nature.com)

Second-line chemotherapy regimens vary, depending on whether hematopoietic stem cell transplantation (HSCT) is being considered. (medscape.com)
Hematopoietic stem cell transplantation remains the only curative therapeutic approach that reliably results in resolution of bone marrow fibrosis in patients with myelofibrosis. (haematologica.org)
3 Myelofibrosis (MF) refers to the Philadelphia chromosome ( BCR-ABL1 )-negative myeloproliferative neoplasm (MPN) originating at the level of the multipotent hematopoietic stem cell. (haematologica.org)
The remarkable potential of stem cells to generate several hundred differentiated cell types is driving their use for regenerative medicine and for supporting the traditional drug discovery and development process (Figure 1). (ddw-online.com)
This article will examine advances taking place on both the lab bench and the pharmaceutical production floor that are helping to bring the promise of stem cells closer to reality. (ddw-online.com)
Inclusion of fetal bovine serum in stem cell media further contributes to an illdefined culture system. (ddw-online.com)
The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)
Identifying the right cocktail of media conditions, supplements and growth factors that successfully drive stem cells toward a desired lineage on a reproducible basis is a time-consuming, iterative exercise. (ddw-online.com)
Solitary cell-derived clonal populations had JNJ-26481585 been retrieved by FACS live sorting and extended in stem cell circumstances. (molecularcircuit.com)
Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)

A total of 7,928 SDE genes and 46 canonical pathways derived from it were identified. (frontiersin.org)
Canonical Wnt/β-catenin signaling is initiated by binding of Wnt ligands to Frizzled (Fzd)-LRP5/6 receptor complexes, leading to inactivation of the destruction complex and stabilization of β-catenin. (plos.org)

Understanding the mechanisms that regulate signaling by these kinases should uncover new ways to inhibit cancer cell growth. (cancer.gov)
Our current work utilizes biochemical and genetic approaches to identify mechanisms that regulate the induction of death by TRAIL ligand in breast and ovarian cancer cells. (cancer.gov)

A stable AEG‑1‑deficient HT22 neuronal cell line was constructed using CRISPR/Cas9 gene‑editing technology. (spandidos-publications.com)
In AST, AEG-1 causes glutamatergic excitotoxicity by downregulating the activity of the excitatory amino acid transporter 2 (EAAT2) promoter, leading to neuronal cell death in glioma-induced neurodegenerative disease ( 10 ). (spandidos-publications.com)

Basal macroautophagy (thereafter referred to as autophagy), a catabolic recycling system in cells, is key to maintaining cellular homeostasis and survival. (hindawi.com)
To investigate cellular and metabolic characteristics associated with these limitations, we compare HEK293 clones producing either erythropoietin (EPO) (secretory) or GFP (non-secretory) protein at different rates. (bvsalud.org)
This enables cells to communicate and respond to external environments, maintain cellular homeostasis, and transduce signals. (mdpi.com)

In summary, the results demonstrated that USF1 binds to the promoter region of thousands of genes and affects a large part of DEGs indirectly. (spandidos-publications.com)

Distinct tumorigenic potential of abl and raf in B cell neoplasia: abl activates the IL-6 signaling pathway. (sagepub.com)

Long noncoding RNA NALT1-induced gastric cancer invasion and metastasis via NOTCH signaling pathway. (nih.gov)

Cloning and characterization of an active fragment of luciferase from a luminescent marine alga, Pyrocystis lunula. (bio.net)

Ly6C low MC manifested activated T-cell signaling pathways and potentially can adapt the function of lymphocytes. (frontiersin.org)
Signaling via IL-2 and IL-4 in JAK3-deficient severe combined immunodeficiency lymphocytes: JAK3-dependent and independent pathways. (sagepub.com)

W hen he began a postdoc in Ronald Davis 's laboratory at Stanford University in 1984, Stephen Elledge wanted to develop new ways to knock out and mutate specific genes in mammals. (the-scientist.com)

Stephen Elledge has built a career studying how eukaryotic cells maintain genomic integrity. (the-scientist.com)
His first experimental results contained a serendipitous artifact that laid the foundation for a scientific career studying how eukaryotic cells deal with damage to their DNA. (the-scientist.com)
Elledge's idea that eukaryotic cells sense the progress of DNA replication and transform that information into a DNA-damage response was new. (the-scientist.com)
All ORFs were cloned into eukaryotic manifestation plasmid pCAGGS along with an N-terminal FLAG tag. (biomasswars.com)

Anatomy10

Organisms3

Chemicals and Drugs21

Analytical, Diagnostic and Therapeutic Techniques and Equipment16

Phenomena and Processes37

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Health Care1

Proliferation25

Differentiation16

Apoptosis20

Proteins15

Molecular14

Downstream7

Inhibition6

MAPK3

Vitro9

Expression53

Molecules6

Several signalin1

Embryonic6

Innate5

Multiple signalin1

Target genes1

Mechanisms and pathways1

Pluripotent4

Immune5

Progenitor cell3

Tissue4

Mutations8

Upstream2

Transduction pathways1

Activate1

Cytokines2

Apoptotic2

Aberrant2

Immunity2

Transcription3

Receptors5

Regulation4

Extracellular2

Mammalian cells1

Mediate2

Regulates2

Transcriptional2

Kinase11

Regulatory3

Tissues1

Alterations1

Chromosomal1

Neurons4

Antibody6

Inhibits3

Interferon2

Adaptive1

Responses5

Tumor cells1

Glial1

Activation9

Promoters1

Stem10

Canonical2

Regulate2

Neuronal2

Cellular3

Binds1

Activates1

NOTCH1

Characterization1

Lymphocytes2

Specific genes1

Eukaryotic4