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  • chromatin
  • By employing human liver chimeric mice and taking advantage of the innovative tools available within the consortium, we plan to determine whether epigenetic changes occur early upon HBV infection and are associated with specific changes of the host chromatin, as well as whether such epigenetic aberrations persist and can even be maintained in human cells that cleared the infection through cell division. (epilog-infect.org)
  • Mechanistically, Gadd45a facilitates the generation of a permissive chromatin state for DDR signaling by inducing base excision repair‐dependent demethylation of CpG islands specifically at sub‐telomeric regions of short telomeres. (embopress.org)
  • 2009 ). This regional specificity is partially explained by different localization of the receptors (Reul and De Kloet 1985 ), but mostly reflects cell type-specific differences in chromatin structure and different subsets of transcriptionally active proteins that interact with MR and GR. (springer.com)
  • somatic cell
  • Although exciting results have been achieved by means of somatic cell nuclear transfer, cell fusion, and culture-induced reprogramming [ 1 ], these procedures are technically demanding and inefficient and therefore unlikely to become a common approach for producing patient-specific pluripotent cells. (biomedcentral.com)
  • MicroRNA cluster 302-367 enhances somatic cell reprogramming by accelerating a mesenchymal-to-epithelial transition," The Journal of Biological Chemistry , vol. 286, no. 19, pp. 17359-17364, 2011. (hindawi.com)
  • Histone
  • These included, among others, inhibition of cell cycle progression but more importantly activation of the histone demethylase pathway. (frontiersin.org)
  • Mesenchymal
  • Multiparameter flow cytometry for the characterisation of extracellular markers on human mesenchymal stem cells. (ebscohost.com)
  • Effects of mesenchymal stromal cell-derived extracellular vesicles on tumor growth. (ebscohost.com)
  • The immunocytochemical profile of these cells was very comparable to that of cells of a mesenchymal, fibroblast/myofibroblast lineage. (hindawi.com)
  • mice
  • When these males were bred with normal female mice, the resulting offspring showed similar depression-like behaviors as adults, even though they were not subjected to stress during their upbringing. (dana.org)
  • Studies will employ an integrated experimental approach that combines different cell transformation models, including primary mammalian cells, humanized mice and adult human stem cells with genome-wide analytical methods to investigate epigenetic changes of the host genome induced as a consequence of HAdV oncogene expression. (epilog-infect.org)
  • Similarly, AAAs induced by angiotensin II (AngII) infusion into mice exhibit a higher prevalence in males. (ahajournals.org)
  • To define the role of developmental testosterone exposures in sexual dimorphism of AAAs, we determined if exposure of neonatal female mice to testosterone confers adult susceptibility to AngII-induced AAAs. (ahajournals.org)
  • Angiotensin II (AngII) infusion into hypercholesterolemic mice increased atherosclerosis and induced formation of aneurysms in both the ascending and suprarenal aortas of male mice. (ahajournals.org)
  • 19 , 20 In mice, these AngII-induced vascular pathologies are mediated through angiotensin type 1a receptors (AT1aR). (ahajournals.org)
  • 21 - 23 Although each of these vascular pathologies are induced by infusion of AngII, only AAAs exhibit marked sexual dimorphism with a higher prevalence (4-fold) in male compared to female mice. (ahajournals.org)
  • 16 , 17 , 24 , 25 Testosterone was determined to be a primary mediator of higher AAA prevalence in adult AngII-infused male mice. (ahajournals.org)
  • 25 , 26 As a mechanistic target of testosterone to promote AngII-induced AAAs, AT1aR mRNA abundance was greater in abdominal compared to thoracic aortas of male but not female mice. (ahajournals.org)
  • Later that year, the lab followed up by further manipulating IPS cells to treat sickle-cell anemia in mice, the first proof in principle of therapeutic use of such cells. (mit.edu)
  • cellular
  • A better understanding of the nature of the cellular damage induced by chronic and episodic heavy (binge) drinking is critical for the improvement of current therapeutic strategies designed to treat patients suffering from alcohol-related disorders. (springer.com)
  • Adult hippocampal neurogenesis is a critical form of cellular plasticity that is greatly influenced by neural activity. (jneurosci.org)
  • Whether serotonin or norepinephrine has a direct effect on adult hippocampal precursors, and the cellular and molecular identity of such a precursor population, therefore remains unknown. (jneurosci.org)
  • mRNA
  • Neonatal testosterone administration increased abdominal aortic AT1aR mRNA abundance and promoted a striking increase in AngII-induced AAAs in adult females exhibiting low serum testosterone concentrations. (ahajournals.org)
  • Increased AT1aR mRNA abundance was maintained during progressive passaging of female smooth muscle cells. (ahajournals.org)
  • tissues
  • Ageing and the physiologic decline of tissues and cells were once thought to be irreversible. (chenglilab.org)
  • In addition, as the genetic identity of the donor egg from which the ESCs are derived most likely will differ from that of potential recipients, patients who receive ESC-derived cells or tissues may face the same complications that result from organ transplantation (for example, immunorejection, graft-versus-host disease, and need for immunosuppression). (biomedcentral.com)
  • In several adult tissues, they retain the ability to self-renew and differentiate into a variety of specialized cells, thus contributing to tissue homeostasis and repair throughout life span. (springer.com)
  • Growing and developing tissues are particularly vulnerable to alcohol's influence, suggesting that stem- and progenitor-cell function could be affected. (springer.com)
  • Accordingly, recent studies have revealed the possible relevance of alcohol exposure in impairing stem-cell properties, consequently affecting organ development and injury response in different tissues. (springer.com)
  • To further elucidate their role in LUAD pathogenesis, we first confirmed abundant expression of protein products of the four members by immunostaining in adult human normal lung tissues. (frontiersin.org)
  • In the long run, IPS cells offer major promise for use in regenerative medicine, potentially supporting the growth of healthy cells and tissues derived from a patient's own cells. (mit.edu)
  • diseases
  • The ability to restore pluripotency to somatic cells through the ectopic co-expression of reprogramming factors has created powerful new opportunities for modelling human diseases. (ebscohost.com)
  • Closer in time, the cells will allow scientists to transfer complex human diseases into Petri dishes for study, taking a first step toward analyzing the conditions and developing therapies. (mit.edu)
  • fate
  • X Chromosome Fate Differs in Mouse, Human iPS Cells. (ebscohost.com)
  • In this review, we provide an overview of this new field and pay particular attention to the impact that single cell transcriptomics is expected to have on our understanding of development and our ability to engineer cell fate. (deepdyve.com)
  • In recent times Waddington's notion of the epigenetic landscape has been rigorously formalized in the context of the systems dynamics state approach to the study of cell-fate. (wikipedia.org)
  • Cell-fate determination is predicted to exhibit certain dynamics, such as attractor-convergence (the attractor can be an equilibrium point, limit cycle or strange attractor ) or oscillatory. (wikipedia.org)
  • promotes
  • Hydroxylation of 5-methylcytosine by TET1 promotes active DNA demethylation in the adult brain. (upenn.edu)
  • Fetal
  • Stem cells drive embryonic and fetal development. (springer.com)
  • This is not surprising, considering that cells belonging to the amniotic epithelium, fetal skin, and the fetal urogenital, respiratory, and gastrointestinal systems have been detected in amniotic fluid. (hindawi.com)
  • In addition, it is also known that the composition, the morphology, and the growth properties of amniotic-fluid cell samples are affected by certain fetal pathologies, such as for example, neural tube defects or gastroschisis [ 1 - 3 ]. (hindawi.com)
  • In 2008, the lab reported that neurons derived from IPS cells successfully integrated into fetal mouse brains and reduced symptoms in a Parkinson's disease rat model. (mit.edu)
  • tumor cells
  • The appearance of H100A14 induced by TPA and KLF4 experienced also been recognized in additional breasts cancer tumor cells such as Testosterone levels47D and SKBR3 and intestines cancer 160335-87-5 IC50 tumor cells HCT116 (data not really Gja7 proven). (chenglilab.org)
  • Similarly, lumbar punctures examining the cerebrospinal fluid for circulating tumor cells are not commonly performed in children with this disease. (oncolink.org)
  • clinical
  • For clinical use, hiPS lines must be reprogrammed from cultured adult cells, and could carry a mutational load due to normal somatic mutation. (chenglilab.org)
  • Only few are clinically relevant inducing severe clinical symptoms under immune suppression. (epilog-infect.org)
  • However, to the best of our knowledge, we are not aware of a report describing the clinical use of such a cell-based therapy approach in humans until now. (hindawi.com)
  • For the purpose of future clinical applications to replace human sensory hair cells, it is ideal to guide stem cells to become sensory hair cells without changing DNA sequence. (frontiersin.org)
  • hematopoietic stem
  • Jaenisch and colleagues [ 10 , 11 ] showed - as proof of principle of the therapeutic potential of iPSCs - the rescue of sickle cell disease and Parkinson disease in mouse models after transplantation of iPSC-derived hematopoietic stem cells and dopaminergic neurons, respectively. (biomedcentral.com)
  • markers
  • Here, a multiparameter flow cytometry assay was developed to examine the expression of several bone marrow-derived hMSC markers simultaneously at the single cell. (ebscohost.com)
  • ESCs
  • The potential use of embryonic stem cells (ESCs) for cell replacement therapies is limited by ethical concerns and the technical hurdles associated with their isolation from human embryos. (biomedcentral.com)
  • Continuous passaging of the iPSCs diminished the differences between iPSCs and ESCs, implying that iPSCs lose the characteristics inherited from the parent cells and adapt to very closely resemble ESCs over time. (prolekare.cz)
  • TET1
  • Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1," Science , vol. 324, no. 5929, pp. 930-935, 2009. (hindawi.com)
  • biology
  • The article discusses a report in "Nature" in which researchers at the RIKEN Center for Developmental Biology in Kobe, Japan, were able to coax embryonic stem cells to develop into retinal cells. (ebscohost.com)
  • The field of stem-cell biology has been catapulted forward by the startling development of reprogramming technology. (ebscohost.com)
  • Barwy Pasażowanie ludzkich embrionalnych komórek macierzystych linii Trypsyna Erin Trish 1 , John Dimos 1 , Kevin Eggan 1 1 Department of Molecular and Cell Biology, Harvard W tym filmie pokazujemy, jak w naszym laboratorium rutynowo fragmenty barwy linii ludzkich zarodkowych komórek macierzystych, z trypsyny. (jove.com)
  • Zamrażanie komórek ES człowieka Erin Trish 1 , John Dimos 1 , Kevin Eggan 1 1 Department of Molecular and Cell Biology, Harvard Tutaj pokazujemy, jak w naszym laboratorium zawiesza barwy linii ludzkich zarodkowych komórek macierzystych. (jove.com)
  • Człowieka komórek ES: Kultura Od mrożone komórki Erin Trish 1 , John Dimos 1 , Kevin Eggan 1 1 Department of Molecular and Cell Biology, Harvard Tutaj pokazujemy, jak w naszym laboratorium rozpoczyna odcienie ludzkich zarodkowych komórek macierzystych kultury linii mrożonych ręki. (jove.com)
  • Transfer jądra do komórki jajowej myszy Dieter Egli 1 , Kevin Eggan 1 1 Department of Molecular and Cell Biology, Harvard Ten film i protokół mają pomóc nauki transfer jądra. (jove.com)
  • Passage TINTEN menselijke embryonale stamcellijnen-lijnen met trypsine Erin Trish 1 , John Dimos 1 , Kevin Eggan 1 1 Department of Molecular and Cell Biology, Harvard In deze video laten we zien hoe onze lab routinematig TINTEN menselijke embryonale stamcellijnen passages met trypsine. (jove.com)
  • Invriezen menselijke embryonale stamcellen Erin Trish 1 , John Dimos 1 , Kevin Eggan 1 1 Department of Molecular and Cell Biology, Harvard Hier laten we zien hoe onze lab TINTEN menselijke embryonale stamcellijnen bevriest. (jove.com)
  • Menselijke ES-cellen: Vanaf Cultuur van bevroren cellen Erin Trish 1 , John Dimos 1 , Kevin Eggan 1 1 Department of Molecular and Cell Biology, Harvard Hier laten we zien hoe onze lab een TINTEN menselijke embryonale stamcellen lijn cultuur begint met een bevroren voorraad. (jove.com)
  • Nuclear Transfer naar Mouse Eicellen Dieter Egli 1 , Kevin Eggan 1 1 Department of Molecular and Cell Biology, Harvard Deze film en het protocol zijn bedoeld om te leren nucleaire transfer. (jove.com)
  • We will discuss here stem cell biology, signaling factors that affect these cells, and the potential contribution of these processes to the etiology of neuropsychiatric disorders. (frontiersin.org)
  • We apologize to colleagues whose work we may not have been able to be included in this review due to space constraints.We thank all of the members of the Laboratory of Molecular Neuroscience, Department of Stem Cell Biology and Medicine, Kyushu University, for their valuable comments, and Elizabeth Nakajima for critical reading of this manuscript. (springer.com)
  • More recently, computational approaches have been critical in the translation of high throughput data into knowledge of both developmental and stem cell biology. (deepdyve.com)
  • In the past several years, a new subdiscipline of computational stem cell biology has emerged that synthesizes the modeling of systems-level aspects of stem cells with high-throughput molecular data. (deepdyve.com)
  • Molecular
  • When using a pattern-matching algorithm, we showed that TBX's overexpression mimic molecular signatures from azacitidine treated NCI-H1299 cells which in turn are inversely correlated to expression profiles of both human and murine lung tumors relative to matched normal lung. (frontiersin.org)
  • differentiate
  • During multimodal treatment, GSCs de-differentiate and convert into cells with malignant characteristics, and thus play critical roles in tumor propagation. (springer.com)
  • stable
  • Transfection and business of stable cell lines were performed as explained previously (22). (chenglilab.org)
  • It is possible to generate monoclonal genomically stable AFS cell lines harboring high proliferative potential without raising ethical issues. (hindawi.com)
  • vitro
  • HBV-mediated epigenetic alterations have been observed in vitro and are commonly found in HBV-related HCCs. (epilog-infect.org)
  • In the present study, we investigated the effects of serotonin and norepinephrine on adult hippocampal precursors in vitro using the neurosphere assay. (jneurosci.org)
  • Therapeutic
  • It acts as a volumizer in cosmetic procedures that provides a matrix for the administration of therapeutic cells or biologics to a patient. (nextbigfuture.com)