Induce differentiation murine differentiation of cells. Medical search. Frequent questions

Genetic or pharmacologic inhibition of CaMK4 decreased the frequency of IL-17-producing T cells and ameliorated EAE and lupus-like disease in murine models. (jci.org)
Inhibition of CaMK4 reduced Il17 transcription through decreased activation of the cAMP response element modulator α (CREM-α) and reduced activation of the AKT/mTOR pathway, which is known to enhance Th17 differentiation. (jci.org)
SR1001 inhibited the development of murine T H 17 cells, as demonstrated by inhibition of interleukin-17A gene expression and protein production. (nature.com)
These results suggested that curcumin effectively regulated the differentiation of naïve, TCM, and TEM cells in the peripheral blood to alleviate DSS-induced experimental colitis, which might be related to the inhibition of JAK1/STAT5 signaling activity. (sciencegate.app)
Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
SFE attenuated the RANKL-mediated induction of NF-κB through inhibition of IκBα phosphorylation, which contributed to inhibiting of RANKL-induced osteoclast differentiation through downregulation of NFATc1. (biomedcentral.com)
Pharmaceutical inhibition of osteoclast differentiation is a current therapeutic approach for the treatment of bone loss-related diseases. (biomedcentral.com)
Publisher Correction: RAGE inhibition blunts insulin-induced oncogenic signals in breast cancer. (medscape.com)

Cyclin-dependent kinase 2-associating protein 1 commits murine embryonic stem cell differentiation through retinoblastoma protein regulation. (nih.gov)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

Sodium butyrate induces histone hyperacetylation and differentiation of murine embryonal carcinoma cells. (rupress.org)
TWS 119 induces neuronal differentiation in pluripotent murine embryonal carcinoma cells and embryonic stem cells at 400 nM. (tocris.com)

Btk is required for the proliferation and differentiation of B lymphocytes. (medscape.com)
Low-level laser therapy has been investigated as a possible stimulus for enhancement of proliferation and differentiation of various cell types, but few reports relate undifferentiated mouse pulp cells (OD-21) response to irradiation in in vitro models. (bvsalud.org)
OGG1 and SMAD7 interact to induce fibroblast proliferation and differentiation and display roles in fibrotic murine and IPF patient lung tissue. (lu.se)

The novel Cymerus™ manufacturing facilitates production of a virtually limitless supply of consistent human induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells, which could play a key role in selective immunosuppression and graft repair during rejection. (biomedcentral.com)

Microglia are immune cells in the CNS, and known to have inflammatory functions via releasing proinflammatory cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-1β 6 . (nature.com)
Isolation of naive T cells from a murine model of lupus revealed increased levels of CaMK4 following stimulation with Th17-inducing cytokines but not following Treg, Th1, or Th2 induction. (jci.org)
Combined stimulation with the T helper cell type 2 cytokines interleukin (IL)-4 and IL-10 induces mouse mast cell apoptosis. (southernbiotech.com)
Furthermore, SR1001 inhibited the expression of cytokines when added to differentiated murine or human T H 17 cells. (nature.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
The inflammasome activates the highly pro-inflammatory cytokines IL-1β and IL-18 and induced programmed cell death-pyroptosis thus inducing inflammation. (tcd.ie)
In this review, we discuss the dualistic effects of histamine: how histamine affects inflammation of the immune system through the activation of intracellular pathways that induce the production of inflammatory mediators and cytokines in different immune cells and how histamine exerts regulatory functions in innate and adaptive immune responses. (hindawi.com)
After 7 days of continuous curcumin (100 mg/kg/day) administration, the DSS-induced experimental colitis was effectively relieved, with significant decreases in the ratio of day weight to initial body weight, colonic weight, pathological injury score, levels of proinflammatory cytokines IL-7, IL-15, and IL-21, colonic mucosal ulceration, and amount of inflammatory infiltrate. (sciencegate.app)
During the process of airway inflammation, complex interactions of innate and adaptive immune cells as well as structural cells and their cytokines have many important roles. (cdc.gov)
T helper (Th) 2 cells, which recruit and accumulate in the lungs and produce a range of different effector cytokines. (cdc.gov)
However, more recent studies have revealed the potential collaboration of other helper T cells and their cytokines in this process. (cdc.gov)
The aim of this review is to summarize the current knowledge about the possible roles of newly identified helper T cells derived cytokines (IL-9, 17, 22, 25 and IL-33) in asthma. (cdc.gov)

Thymic atrophy induced in the fetus correlated with caspase-dependent apoptosis in thymocytes. (aspetjournals.org)
Thymic atrophy was the result of direct action of THC and not based on maternal factors inasmuch as THC was able to induce T cell apoptosis in vitro in fetal thymic organ cultures. (aspetjournals.org)
Chronic myeloid leukemia (CML) results from BCR-ABL oncogene, which blocks CML cells differentiation and protects these cells from apoptosis. (jcancer.org)
Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
Yet another group of genes associated with cancer formation regulates programmed cell death, or apoptosis. (the-scientist.com)
The importance of apoptosis is exemplified by the vertebrate adaptive immune system, in which B lymphocytes, as they differentiate from their precursor cells, rearrange the DNA of immunoglobulin genes to generate a vast number of potential antibodies. (the-scientist.com)
HQ reduced the proliferation and the differentiation and colony formation, but increased the apoptosis of both YS-HSCs and BM-HSCs. (cdc.gov)
RHAMM regulates MMTV-PyMT-induced lung metastasis by connecting STING-dependent DNA damage sensing to interferon/STAT1 pro-apoptosis signaling. (medscape.com)

Benzimidazoisoquinolines: a new class of rapidly metabolized aryl hydrocarbon receptor (AhR) ligands that induce AhR-dependent Tregs and prevent murine graft-versus-host disease. (oregonstate.edu)

It was also reported to be involved in pro-inflammatory or anti-inflammatory processes, as well as in regulatory T cells differentiation. (conicet.gov.ar)
Here, we investigatedthe role of Gal-8 in the murine inflammatory chronic infection induced by T. cruzi (4 months postinfection). (conicet.gov.ar)
The inflammatory response is the coordinated activation of signalling pathway leading to immune cell recruitment into the site of infection and production of inflammatory mediators. (tcd.ie)
Findings suggest that HMGB1 is a potential target for early-stage OAC, and that blocking TLR2 signalling may limit HMGB1 release, inflammatory cell infiltration and inflammation during OAC progression. (tcd.ie)
Previously, we showed that exosomes derived from human adipose tissue-derived mesenchymal stem cells (ASC-exosomes) attenuate AD-like symptoms by reducing multiple inflammatory cytokine levels. (mdpi.com)
Notably, imbalances in Tfh differentiation often lead to the development of autoimmune diseases, including inflammatory bowel disease (IBD). (sciencegate.app)
Fibrotic livers are characterized by an inflammatory microenvironment that is composed of various immunologically active cells, including liver-resident populations (e.g. (mdpi.com)
He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
In addition, TH5487 decreases levels of pro-inflammatory mediators, inflammatory cell infiltration, and lung remodeling in a murine model of bleomycin-induced pulmonary fibrosis conducted in male C57BL6/J mice. (lu.se)

IL-10 inhibits FcεRI expression in mouse mast cells. (southernbiotech.com)
Here we present SR1001, a high-affinity synthetic ligand-the first in a new class of compound-that is specific to both RORα and RORγt and which inhibits T H 17 cell differentiation and function. (nature.com)
We show that BO and early-stage OAC cells were responsive to TLR2 stimulation and TLR2 neutralising antibody successfully inhibits TLR2-mediated chemokine production. (tcd.ie)
Ectopic expression of AML1-MTG8 in L-G murine myeloid progenitor cells inhibits differentiation to mature neutrophils and induces cell proliferation in response to granulocyte colony-stimulating factor (G-CSF). (embl-heidelberg.de)
It also inhibits Shh induced Gli luciferase reporter activity in Shh-light 2 cells. (americanchemicalsuppliers.com)

Ngns (class II bHLH transcription factors) are known as pro-neural factors as they are necessary to initiate differentiation of NSPCs and are important to specify a neuronal subtype[3]. (bartleby.com)
Thoma et al observed that Ngn2 alone is sufficient to induce neuronal differentiation in embryonic stem cells. (bartleby.com)
In this study they used murine embryonic stem cells and transfected with Ngn2 expression and after five days post transfection they observed that cells expressed Tuj1 and MAP2 (both are neuronal markers). (bartleby.com)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)

Methods: To model genetic alterations in the core signaling pathways of human glioblastoma, we induced conditional Rb knockout, Kras activation, and Pten deletion mutations in cortical murine astrocytes. (nih.gov)
Serial neurosphere culture, multi-lineage differentiation, and orthotopic transplantation were used to assess whether these mutations induced de-differentiation of cortical astrocytes into glioma stem cells. (nih.gov)
Results: G1/S-defective, Rb knockout astrocytes gained unlimited self-renewal and multi-lineage differentiation capacity, in both the presence and absence of Kras and Pten mutations. (nih.gov)
Conclusion: A defined set of core signaling pathway mutations induces de-differentiation of cortical murine astrocytes into glioma stem cells. (nih.gov)
Murine gene mutations in human counterparts may be associated with a clinical illness different from the illness seen in mice. (medscape.com)

From the scientific articles released at that time that he was the Chief Author of, the research areas included colony-stimulating factors (CSF) and their relevance to white blood cells and the immune response in vitro. (wikipedia.org)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
Soluble Axl (sAxl) is present in cell-conditioned medium of CUDC-305 (DEBIO-0932 ) tumor cells growing in vivo and in vitro and in the sera of humans mice and rats (23 50 However the identities of the sAxl-generating protease(s) and the mechanism(s) that account for this process remain unknown. (exposed-skin-care.net)
5E3 inhibited LPS-dependent cell activation in vitro and prevented proinflammatory cytokine production in vivo following LPS challenge in a dose-dependent manner. (tum.de)
Second, altering the MSC state by inducing differentiation into multiple lineages did not affect the exosomal properties or endocytosis but triggered the expression of lineage-specific genes and proteins in vitro and in vivo respectively. (frontiersin.org)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)

Immortalized murine pulp cells (OD-21)6 are considered as the major action mechanism of those lasers.11 undifferentiated mesenchymal stem cells derived from fetal Although this mechanism has not been fully clarified, the most molar papillae7. (bvsalud.org)
Mesenchymal stem cells (MSCs) are multipotent cells with regenerative and immunomodulatory properties. (frontiersin.org)
Human mesenchymal stem cells (HMSCs) are multipotent somatic stem cells that can be isolated from a variety of tissues such as the bone marrow, adipose tissue, and dental pulp. (frontiersin.org)

Aptamers are single-stranded pieces of DNA that bind to target proteins and are able to modulate signaling cascades during cell differentiation when a stem cell commits to a specific functional role or phenotype. (shinshu-u.ac.jp)
iSN04 treatment under differentiating conditions inhibited stem cell commitment to the cardiac lineage. (shinshu-u.ac.jp)
Cell Stem Cell. (ucsf.edu)
TWS 119 enhances the proliferation and survival of γδ T-cells, and induces differentiation of CD8+ T cells towards a stem cell-like memory T cell phenotype at 2.5 μM. (tocris.com)
Embryonic stem cells have been identified by scientist as a type of stem cell that can advance regenerative medicine. (bartleby.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Stem cell-based immunotherapy has been recognized as a crucial immunoregulatory regimen in various preclinical and clinical studies. (biomedcentral.com)
There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
He is a member of the Stem Cell Network Centre of Excellence (past member of the Stem Cell Policy Committee and Research Management Committee and current Sub-chair of the Training and Education Committee), and a member of the AllerGen Network Centre of Excellence (Research Management Committee and Co-Chair of the Biomarkers Program). (ubc.ca)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)

The latter observations suggest that sodium butyrate has the ability to reactivate a silent cRABP gene in PCC4(RA)-1 cells and thereby lead to extensive differentiation via the retinoid pathway when RA is added. (rupress.org)
Using various assays like RNA sequencing, cell staining and imaging, and molecular interaction and pathway analysis, the researchers investigated iSN04's effect on murine ESCs and iPSCs. (shinshu-u.ac.jp)
Second, it results in the modulation of the Wnt signaling pathway that governs cell differentiation. (shinshu-u.ac.jp)
The iNOS-induced nitric oxide production is regulated through IFNAR/JAK/STAT1 pathway. (tcd.ie)
In a previous study, we also reported that 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MMP-9 expression is inhibited by silibinin through inactivation of the RAF/MEK/ERK pathway ( 6 ), as well as augmenting the cell cycle arrest at the G 2 /M phase through the induction of p21 and the reduction of cyclin B1 in breast cancer cells ( 7 ). (iiarjournals.org)
In this study, we investigated the effect of silibinin on EGFR signaling pathway and EGF ligand-induced CD44 expression in breast cancer cells. (iiarjournals.org)

Differentiation of human and murine induced pluripotent stem cells to microglia-like cells. (alzforum.org)

Furthermore, we have analyzed the gene expression paterns of iMG cells in both manic and depressive state of patients with bipolar disorders, and revealed state-dependent microglial experession patterns 16 . (nature.com)
IL6 induces neurogenesis-related gene expression in the hypothalamus and in neuroprogenitor cells, both from WT as well as from KO mice. (nih.gov)
IL6 induces neurogenesis-related gene expression in the hypothalamus of WT mice. (nih.gov)
He performed some of the first studies to identify transcription factors that regulate the gene expression and differentiation of eosinophils, which are known to play a major role in allergic and asthmatic responses. (ubc.ca)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

He also researches the developing nervous system and the different roles that proteins, white blood cells and genes play. (wikipedia.org)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
Several studies shown that Axl could mediate cell adhesion and aggregation through homotypic ectodomain associations (9 23 Both CUDC-305 (DEBIO-0932 ) murine and human being Axl proteins undergo proteolytic processing to yield a soluble form of this molecule. (exposed-skin-care.net)
The normal function of the proteins expressed by these genes is to recognize superfluous, damaged, aged, or aberrant cells that must be eliminated. (the-scientist.com)
Even though the exact insight mechanisms of how ascitic fluid exert to ovarian cancer cells is poorly defined, my research suggests that ascitic fluid can increase expression of oncogenic proteins in ovarian cancer cells and reduce the cellular uptake of targeted drugs. (otago.ac.nz)
Many existing tissue-engineering approaches focus on delivery of selected proteins (growth factors, transcription factors etc.) or nucleic acids to host or implanted stem cells to achieve lineage specific differentiation. (frontiersin.org)
There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. (who.int)

designated IL-17A-F. IL-17 is expressed by a unique lineage of CD4 T cells, Th17 cells. (biolegend.com)
IL-23, which also activates Stat3, serves to maintain Th17 cells lineage. (biolegend.com)
Here, we present evidence that the calcium/calmodulin-dependent protein kinase IV (CaMK4) is increased and required during Th17 cell differentiation. (jci.org)
Importantly, silencing CaMK4 in T cells from patients with SLE and healthy individuals inhibited Th17 differentiation through reduction of IL17A and IL17F mRNA. (jci.org)
McGeachy, M. J. & Cua, D. J. Th17 cell differentiation: The long and winding road. (nature.com)
Littman, D. R. & Rudensky, A. Y. Th17 and regulatory T cells in mediating and restraining inflammation. (nature.com)
Th17 cell may have a role in severe asthma and chronic obstructive pulmonary disease (COPD). (cdc.gov)

iGal-8KO mice displayed anincreased development of splenomegaly (evaluated by cellularity and weight) compared to iWT.Spleen cells analysis by Flow-cytometry showed no percentage differences among CD4 Tlymphocytes, myeloid cells and their different subpopulations, between iGal-8KO and iWT. (conicet.gov.ar)

Generating specific cell lineages from induced pluripotent stem cells and embryonic stem cells is the holy grail of regenerative medicine. (shinshu-u.ac.jp)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)

Begley was also involved in more work with mice that found that bone marrow cells are not a contributor to endothelium of tumors. (wikipedia.org)
Splenic endothelial cell lines support development of dendritic cells from bone marrow. (southernbiotech.com)
In this study, we examined the potential of SF extract (SFE) to inhibit receptor activator of NF-κB ligand (RANKL) -induced osteoclast differentiation in cultured mouse-derived bone marrow macrophages (BMMs). (biomedcentral.com)
Comparison of toxicity of benzene metabolite hydroquinone in hematopoietic stem cells derived from murine embryonic yolk sac and adult bone marrow. (cdc.gov)
In the current study, we compared the effects of hydroquinone (HQ), a major metabolite of benzene in humans and animals, on mouse embryonic yolk sac hematopoietic stem cells (YS-HSCs) and adult bone marrow hematopoietic stem cells (BM-HSCs). (cdc.gov)

Factors secreted from TLR2-activated oesophageal cells induce TLR2-mediated differentiation of murine macrophages into M2-like/TAM phenotype. (tcd.ie)
We also determined that Caspase-11 is required for restriction of Mtb proliferation in murine macrophages. (tcd.ie)
We show that primary murine macrophages infected with H. pylori upregulate caspase-11 (the orthologue of human caspase-4), activate caspase-1 and secrete IL-1β. (tcd.ie)
CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes. (thermofisher.com)
These cells, described as macrophages or immature dendritic cells, deplete arginine and impair T cell proliferation and cytokine production. (aacrjournals.org)
Cell separation studies showed that the increased arginase activity was limited to a specific subset of CD11b+, CD14−, CD15+ cells with a polymorphonuclear granulocyte morphology and markers, instead of macrophages or dendritic cells described in mouse models. (aacrjournals.org)
Kupffer cells, hepatic stellate cells and sinusoidal endothelium) and infiltrating leukocytes (e.g., monocytes, monocyte-derived macrophages, neutrophils and lymphocytes). (mdpi.com)

Upon immobilization sAxl advertised cell migration and induced the phosphorylation of Axl and phosphatidylinositol 3-kinase. (exposed-skin-care.net)
In addition, silibinin suppressed the EGF-induced phosphorylation of EGFR and extracellular signal-regulated kinase1/2 (ERK1/2), a downstream signaling molecule of EGFR. (iiarjournals.org)

Activation of immune cells in the brain called microglia has attracted attention as a potential underlying pathological mechanism in chronic pain. (nature.com)
These rodent studies have indicated the over-activation of microglial cells in patients with chronic pain, however clinical molecular data are lacking due to ethical and technical issues. (nature.com)
TLR2 is involved in the innate immune response to microbial pathogens and host-derived molecules.We assume that TLR2 upregulation will increase the sensitivity of oesophageal cells to bacteria thus leading to chronic inflammation. (tcd.ie)
Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)

Age-Dependent Niche Signals from the Choroid Plexus Regulate Adult Neural Stem Cells. (ucsf.edu)
Zhang, F. P., Meng, G. X. & Strober, W. Interactions among the transcription factors Runx1, RORγt and Foxp3 regulate the differentiation of interleukin 17-producing T cells. (nature.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
Follicular helper T cells (Tfh) regulate the differentiation of germinal center B cells and maintain humoral immunity. (sciencegate.app)
Normal function is to regulate cell division. (the-scientist.com)

Memory of Inflammation in Regulatory T cells. (ucsf.edu)
The ability of certain AhR ligands to induce regulatory T cells (Tregs) has generated interest in developing AhR ligands for therapeutic treatment of immune-mediated diseases. (oregonstate.edu)
BALB/c → C57BL/6 allografts were reconstituted with iPSC-derived MSCs (2 million/transplant/at d0), and allografts were examined for regulatory T cells (Tregs), oxygenation, microvascular blood flow, airway epithelium, and collagen deposition during rejection. (biomedcentral.com)

Aberrant high expression of B lymphocyte chemokine (BLC/CXCL13) by C11b + CD11c + dendritic cells in murine lupus and preferential chemotaxis of B1 cells towards BLC. (southernbiotech.com)
Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)

Vaccinia virus induces strong immunoregulatory cytokine production in healthy human epidermal keratinocytes: a novel strategy for immune evasion. (southernbiotech.com)
These patients had a markedly decreased cytokine production and expressed low levels of T cell receptor CD3ζ chain. (aacrjournals.org)

The excessive activation of AhR resulted in neural differentiation of Neuro2a cells. (biomedcentral.com)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)

Peripheral T cells from such mice showed decreased proliferative response to T cell mitogen as well as both T cell and antibody response to HIV-1 p17/p24/gp120 antigens. (aspetjournals.org)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
However, murine models, including knockout and transgenic mice, have been useful in understanding the mechanisms of B lymphopoiesis, B-cell differentiation, and antibody formation. (medscape.com)

Targeted delivery of tumor necrosis factor-α to tumor vessels induces a therapeutic T cell-mediated immune response that protects the host against syngeneic tumors of different histologic origin. (southernbiotech.com)
Inflammation is an essential immune system response to pathogens, damaged cells and stress stimuli and has an essential role in tissue repair and regeneration. (tcd.ie)
Numerous host innate immune responses are induced upon M.tuberculosis (Mtb), although their mechanisms and impact on mycobacterium are not well understood. (tcd.ie)
As nitric oxide is well known to confine the growth of Mtb we hypothesise that Mtb-induced caspase-11 increases iNOS expression and nitric oxide production and is a crucial protein in the innate immune response to TB-induced pathogen. (tcd.ie)
Together, our data provide strong support for development of select BBQs that activate the AhR to induce Tregs for treatment of immune-mediated diseases. (oregonstate.edu)
Together, our data demonstrate for the first time that perinatal exposure to THC triggers profound T cell dysfunction, thereby suggesting that the offspring of marijuana abusers who have been exposed to THC in utero may be at a higher risk of exhibiting immune dysfunction and contracting infectious diseases including HIV. (aspetjournals.org)
But nonviral tumors, which are composed of aberrant host cells, do not provide such targets, and the immune response is suppressed by defenses against autoimmune reactions. (the-scientist.com)
Specific bacterial products potently stimulate immune cells via toll-like receptors (TLRs). (tum.de)

However, when these pluripotent stem cells were treated after experiencing differentiating conditions for five days, specific marker genes were upregulated, and the cells committed to forming beating cardiomyocytes. (shinshu-u.ac.jp)
In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
Like TCDD,10-Cl-BBQ altered donor CD4(+) T cell differentiation during the early stages of a graft versus host (GVH) response resulting in expression of high levels of CD25, CTLA-4 and ICOS, as well as several genes associated with Treg function. (oregonstate.edu)
Osteoclast differentiation marker genes were analyzed by the quantitative real-time polymerase chain reaction analysis. (biomedcentral.com)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)

In 1999 he released an article which he was the co-author of that was solely on SCL and the relationship with T cells and T lymphocytes. (wikipedia.org)
CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. (thermofisher.com)
The standard form of CD44 is usually ubiquitously expressed on epithelial cells and lymphocytes, but inclusion of one or more of 10 variant exons produces tissue-specific multiple variant isoforms (CD44v2-v10) by alternate mRNA splicing ( 9 ). (iiarjournals.org)

An anti-nucleolin DNA aptamer, iSN04, regulates cardiomyogenesis of murine pluripotent stem cells. (shinshu-u.ac.jp)

Expression of cell-cycle regulator CDK2-associating protein 1 (p12CDK2AP1) in transgenic mice induces testicular and ovarian atrophy in vivo. (nih.gov)
3. Radu DL, Weksler ME, Bona CA. Maintenance of size and function of influenza virus hemagglutinin specific transgenic T-cell clone during life. (southernbiotech.com)

The undifferentiated murine neuroblastoma Neuro2a cell line (ATCC) was stably transfected with AhR cDNA and the established cell line was named N2a-Rα. (biomedcentral.com)

We prepared hypothalamic neuroprogenitor cells from KO that were treated with IL6 in order to provide an ex vivo model to further characterizing the neurogenic actions of IL6 through differentiation assays. (nih.gov)
In addition, ELISA, Biacore, and in-cell FRET assays confirmed the competition between GHCer and PLCß1 for binding to TRAX. (bvsalud.org)

SR1001 binds specifically to the ligand-binding domains of RORα and RORγt, inducing a conformational change within the ligand-binding domain that encompasses the repositioning of helix 12 and leads to diminished affinity for co-activators and increased affinity for co-repressors, resulting in suppression of the receptors' transcriptional activity. (nature.com)
A common heterophilic ligand for these RTK family members is definitely Gas6 a vitamin K-dependent protein that is widely secreted by most cells including the lungs intestine Serping1 and vascular endothelium (43). (exposed-skin-care.net)
To this end, we designed a screen for novel Treg-inducing compounds based on our understanding of the mechanisms of Treg induction by the well-characterized immunosuppressive AhR ligand, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). (oregonstate.edu)
We screened a ChemBridge small molecule library and identified 10-chloro-7H-benzimidazo[2,1-a]benzo[de]Iso-quinolin-7-one (10-Cl-BBQ) as a potent AhR ligand that was rapidly metabolized and not cytotoxic to proliferating T cells. (oregonstate.edu)
CYP1A1, a typical XRE-regulated gene, mRNA was induced without the application of ligand to the culture medium. (biomedcentral.com)
Here, we investigated the effect of silibinin (a polyphenolic flavonolignan of the herbal plant of Silybum marianum, milk thistle) on the epidermal growth factor (EGF) ligand-induced CD44 expression in human breast cancer cells. (iiarjournals.org)
In contrast, EGFR ligand-induced CD44 expression was reduced by EGFR inhibitors, AG1478 and lapatinib, respectively. (iiarjournals.org)
Interestingly, we observed that EGFR ligand-induced CD44 and matrix metalloproteinase-9 (MMP-9) expression was reduced by silibinin treatment in a dose-dependent manner. (iiarjournals.org)
Here, we also studied the effect of silibinin on EGFR ligand-induced CD44 expression, which is a hallmark of metastasis in various cancer cells. (iiarjournals.org)

Significant changes in the percentages of naïve, central memory T (TCM), and effector memory (TEM) cells and their CD4+ and CD8+ subsets were found in the peripheral blood of mice with colitis using flow cytometry. (sciencegate.app)

The Treg phenotype required AhR expression in the donor CD4(+) T cells. (oregonstate.edu)

Podoplanin (PDPN), a 36-kDa type I transmembrane O-glycoprotein, is expressed in normal cells, including renal epithelial cells (podocytes), lymphatic endothelial cells, and pulmonary type I alveolar cells, and in cancer cells, including brain tumors and squamous cell lung carcinomas. (bvsalud.org)

Lupus erythematosus is a heterogeneous connective-tissue disease associated with polyclonal B-cell activation and is believed to result from the interplay of genetic, environmental, and hormonal factors. (medscape.com)
Chemicals such as L-canavanine, which is present in alfalfa sprouts, have been known to induce systemic lupus erythematosus-like illness. (medscape.com)
Drugs implicated in inducing a lupus erythematosus-like illness (eg, procainamide, isoniazid, hydralazine) typically do not induce acute cutaneous lupus erythematosus. (medscape.com)
See also Bullous Lupus Erythematosus, Discoid Lupus Erythematosus, Drug-Induced Lupus Erythematosus, and Subacute Cutaneous Lupus Erythematosus. (medscape.com)

Cells from embryonal carcinoma (EC) lines 6050AJ and PCC4.aza 1R differentiate in response to treatment with sodium butyrate as well as retinoic acid (RA) or hexamethylenebisacetamide (HMBA). (rupress.org)
These results might indicate that the mechanism by which sodium butyrate promotes differentiation of EC cells is different from the ways in which RA and HMBA act. (rupress.org)

Although MSC EVs show great promise for regenerative medicine applications, insights into their uptake mechanisms by different target cells and the ability to control MSC EV properties for defined function in vivo have remained elusive knowledge gaps. (frontiersin.org)

Ultimately, terminal cell differentiation commits to the cardiomyocyte lineage. (shinshu-u.ac.jp)

In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
IL6 expression in the hypothalamus is low and restricted to microglia and tanycytes, whereas IL6 receptor is expressed in microglia, ependymocytes, endothelial cells, and astrocytes. (nih.gov)
BACKGROUND: In endothelial cells, phospholipase C (PLC) ß1-activated Ca2+ is a crucial second messenger for the signaling pathways governing angiogenesis. (bvsalud.org)
Angiogenic activities of GSLs in human umbilical vein endothelial cells (HUVECs) were evaluated. (bvsalud.org)
CONCLUSIONS: Overall, the fucose residue in GHCer dictated the glycan conformation for its complexing with TRAX to release TRAX-sequestered PLCß1, leading to Ca2+ mobilization in endothelial cells and enhancing angiogenesis in tumor microenvironments. (bvsalud.org)

Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)

However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

Mouse IL-6 induced by mIL-17A in fetal mouse skin fibroblasts. (biolegend.com)
We noted that murine fetal thymus expressed high levels of the cannabinoid receptors CB1 and CB2. (aspetjournals.org)

We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)

The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
Despite initial therapeutic outcomes, conventional stem cells face key limitations. (biomedcentral.com)

Foxp3 was not expressed in the AhR-induced Tregs implicating AhR as an independent transcription factor for Treg induction. (oregonstate.edu)
Structure-activity studies showed that unsubstituted BBQ as well as 4, 11-dichloro-BBQ were capable of inducing AhR-Tregs. (oregonstate.edu)

Interleukin (IL)-9-producing subset called Th9 cell, Th22 cells which primarily secrete IL-22, IL-13 and tumor necrosis factor- and Th25 cells via producing IL-25 are believed to be important for initiating allergic reactions and developing airway inflammation. (cdc.gov)

JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)

Differentiation-defective PCC4(RA)-1 EC cells fail to respond to RA, presumably because they possess minimal amounts of active binding protein for RA (cRABP). (rupress.org)
The expression of tumor necrosis factor (TNF)-α at mRNA and protein levels significantly increased in ATP-stimulated iMG cells from patients with fibromyalgia compared to cells from healthy individuals. (nature.com)
We identify High Mobility Group Box 1 protein (HMGB1) as one of the factors secreted from TLR2-stimulated oesophageal adenocarcinoma cells. (tcd.ie)
Histamine and its receptors represent a complex system of immunoregulation with distinct effects mediated by four GPCRs (G protein-coupled receptors HRs 1-4) and their differential expression, which changes according to the stage of cell differentiation and microenvironmental influences. (hindawi.com)
To neutralize LPS signaling in experimental models of sepsis, we generated mAbs toward the TLR4/myeloid differentiation protein-2 (MD-2) complex. (tum.de)
The levels of CD44 mRNA and protein expression were greatly increased by EGF and by TGF-α in SKBR3 and BT474 breast cancer cells. (iiarjournals.org)
protein patterns of subjects with leish- Cell culture maniasis [17]. (who.int)

Manel, N., Unutmaz, D. & Littman, D. R. The differentiation of human TH-17 cells requires transforming growth factor-β and induction of the nuclear receptor RORγt. (nature.com)
Analysis with C-terminal deletion mutants of AML1-MTG8 indicated that the region of 51 residues (488 to 538), which contains NHR2, is essential for the induction of G-CSF-dependent cell proliferation. (embl-heidelberg.de)

Abnormal cell division is a hallmark of tumor cells. (the-scientist.com)
Silibinin ( Figure 1 ) is a polyphenolic flavonolignan of the herbal plant of Silybum marianum, milk thistle, and has a wide range of pharmacologic effects, such as antihepatotoxic and anticarcinogenic in a variety of tumor cells ( 4 , 5 ). (iiarjournals.org)
Although arginase activity has been described in cancer patients, it is thought to originate from tumor cells metabolizing arginine to ornithine needed to sustain rapid cell proliferation. (aacrjournals.org)

It is believed that benzene and its metabolites target hematopoietic stem cells (HSCs) to cause toxicity and cancer in the hematopoietic system. (cdc.gov)

Interestingly, there was a moderate correlation between ATP-induced upregulation of TNF-α expression and clinical parameters of subjective pain and other mental manifestations of fibromyalgia. (nature.com)
Treatment efficacy may depend on the presence of glioma stem cells, expression of DNA repair enzymes such as methylguanine methyltransferase (MGMT), or transcriptome subtype. (nih.gov)
Ectopic expression of Ngn2 was sufficient to form mature neurons from embryonic stem cells[5]. (bartleby.com)
In the mouse, the differentiation of this new lineage is initiated by TGFbeta-1 and IL-6 or IL-21, which activates Stat3 and induces the expression of the transcription factor retinoic acid-related orphan receptors (RORγt and RORα). (biolegend.com)
The activation of exogenous AhR in N2a-Rα cells was confirmed using RNAi, with si-AhR suppressing the expression of exogenous AhR. (biomedcentral.com)
Morphologically, N2a-Rα cells bore spontaneous neurites exhibiting axon-like properties with the localization of NF-H. In addition, cdc42 expression was increased in comparison to the control cell line. (biomedcentral.com)
SFE inhibited the RANKL-induced expression of nuclear factor of activated T cells c1 (NFATc1). (biomedcentral.com)
This project is investigating the expression and activation of tyrosine kinases in ovarian cancer cells isolated from ascitic fluid. (otago.ac.nz)
We used cell labeling with Bromodeoxyuridine (BrdU), immunofluorescence, and real-time PCR to determine the expression of markers of neurogenesis and neurotransmitters. (nih.gov)
In addition, we analyzed single-cell RNA sequencing data and determined the expression of IL6 and IL6 receptor in specific cell types of the murine hypothalamus. (nih.gov)
Expression of FUTs is upregulated in renal cell carcinoma, bladder cancer, and prostate cancer. (bvsalud.org)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)

We had previously found that iSN04 promoted myogenic precursor cells (myoblasts) to differentiate into skeletal muscle cells and had hypothesized that the aptamer also enhanced differentiation of pluripotent stem cells. (shinshu-u.ac.jp)

To overcome this limitation, we have recently developed a technique to create human-induced microglia-like (iMG) cells from human peripheral blood monocytes. (nature.com)
Peripheral blood mononuclear cells from 123 patients with metastatic renal cell carcinoma, prior to treatment, were found to have a significantly increased arginase activity. (aacrjournals.org)

The orphan nuclear receptor RORγt directs the differentiation program of proinflammatory IL-17 + T helper cells. (nature.com)

IL-17A was initially identified from a subtracted cDNA library between closely related murine lymphoid cells and called CTLA-8. (biolegend.com)
In the absence of mature B cells, patients lack lymphoid tissue and fail to develop plasma cells, the cells that manufacture antibodies. (medscape.com)
Germinal centers where B cells proliferate and differentiate are poorly developed in all lymphoid tissue, including the spleen. (medscape.com)

This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)

The goal of this study was to determine whether myeloid suppressor cells producing high arginase existed in renal cell carcinoma patients. (aacrjournals.org)

N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer. (thermofisher.com)

To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)

Anatomy63

Organisms13

Diseases9

Chemicals and Drugs86

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes57

Disciplines and Occupations1

Information Science4

Inhibition8

Embryonic stem2

Embryonal carcinoma cells2

Proliferation and differentiation3

Pluripotent stem1

Cytokines12

Apoptosis8

Aryl hydrocar1

Inflammatory9

Inhibits5

Neuronal4

Mutations5

Vitro6

Mesenchymal stem3

Stem cell16

Pathway6

Microglia-like cells1

Gene expression5

Proteins10

Th177

Myeloid cells1

Lineages2

Bone marrow5

Macrophages7

Phosphorylation2

Chronic4

Regulate5

Regulatory T cel3

Chemotaxis2

Cytokine production2

Neural differentiation2

Antibody3

Immune8

Genes5

Lymphocytes3

Regulates1

Transgenic2

Neuroblastoma1

Assays2

Ligand9

Effector1

Phenotype1

Epithelial1

Lupus4

HMBA2

Vivo1

Commits1

Endothelial6

Lineage specific1

Pathways2

Fetal2

Inhibited the proliferation1

Therapeutic4

Tregs2

Interleukin1

Inhibitors1

Protein7

Induction2

Tumor cells3

Hematopoietic stem1

Expression15

Precursor cells1

Peripheral2

Proinflammatory1

Lymphoid3

Human pluripotent3

Renal cell carc1

Dendritic cells1

Progenitor cells1