Induce differentiation cell differentiation. Medical search. Frequent questions

This study was therefore aimed at establishing the dose-response effect of EPO stimulation on rat BMSCs and investigating the effects of mechanical stretch combined with EPO on the proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
The proliferation and osteogenic differentiation of rat BMSCs were examined and compared using EPO with different concentrations. (hindawi.com)
The osteogenic differentiation of BMSCs was detected by alkaline phosphatase (ALP) staining, and the mRNA expression levels of ALP, OCN, COL, and Runx2 were detected by qPCR. (hindawi.com)
Mechanical stretch combined with 20 IU/ml EPO promoted proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
EPO was able to promote the proliferation and osteogenic differentiation of BMSCs, and these effects were enhanced when combined with mechanical stretch. (hindawi.com)
Notably, it promotes the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) [ 15 - 17 ]. (hindawi.com)
This study was therefore aimed at addressing three key issues: First, is there a dose-dependent effect of EPO on the proliferation and osteogenic differentiation of BMSCs? (hindawi.com)
Reversibly immortalized human umbilical cord-derived mesenchymal stem cells (UC-MSCs) are responsive to BMP9-induced osteogenic and adipogenic differentiation. (stembook.org)
In this study, we examined the effects of FAK point mutations at tyrosine residues (Y397, Y925, Y861, and Y576/7) on osteogenic differentiation of human mesenchymal stem cells exposed to collagen I and cyclic tensile strain. (techscience.com)
Our results demonstrate that FAK signaling emanating from Y397, Y925, and to a lesser extent Y576/7, but not from Y861, controls osteogenic differentiation through an ERK1/2 pathway, as measured by expression levels of key osteogenesis marker genes and subsequent matrix mineralization. (techscience.com)
These data indicate that FAK is a critical decision maker in extracellular matrix/strain-enhanced osteogenic differentiation. (techscience.com)
Long noncoding RNAs (lncRNAs) are emerging regulators for osteogenic differentiation from AMSCs. (aging-us.com)
In the current study, we found that lncRNA-PAGBC was a specific lncRNA that significantly upregulated during osteogenic differentiation of AMSCs based on published database. (aging-us.com)
HP increased lncRNA-PAGBC, which is a competitive endogenous RNA (ceRNA) that binds to the osteogenesis-inhibitory microRNA, miR-133b, to regulate osteogenic differentiation of AMSCs. (aging-us.com)
Suppression of RUNX2 by miR-133b caused impaired osteogenic differentiation of AMSCs. (aging-us.com)
Together, these data suggest that HP induces osteogenic differentiation of AMSCs through increasing lncRNA-PAGBC. (aging-us.com)
To accelerate their osteogenic differentiation, we overexpressed the osteogenic transcription factor Cbfa1 (Core binding factor alpha-1, Til-1) gene using an adenovirus vector in rat ASCs. (jarde.jp)
These findings suggested that overex-pression of the Cbfa1 gene induced a remarkable and rapid differentiation of ASCs into osteogenic cells. (jarde.jp)
Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)

The effects of erythropoietin (EPO) on the behaviors of bone marrow mesenchymal stem cells (BMSCs) subjected to mechanical stretch remain unclear. (hindawi.com)
Mesenchymal stem cell medium was used in the control groups. (scielo.br)
Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
Mesenchymal stem cells (MSCs) are defined by their ability to self-renew and differentiate into the cells that form mesodermal tissues such as bone and fat. (elsevierpure.com)
We differentiated hiPSCs directly into mesenchymal stem /stromal cells (MSC) and chondrocytes. (mdc-berlin.de)
Induced osteogenesis of adipose-derived mesenchymal stem cells (AMSCs) has been used to facilitate bone regeneration. (aging-us.com)
A specific population of adipose-derived stem cells (ASCs) is expected to be an alternate source of bone marrow-derived mesenchymal stem cells (BMSCs). (jarde.jp)

We also observed that ASK1DeltaN induced expression of several neuron-specific proteins and phosphorylation of neurofilament proteins, confirming that PC12 cells differentiated into mature neuronal cells by ASK1. (nih.gov)
We have examined NGF-induced signal transduction events and neuronal differentiation in MAH cells, a neuronal progenitor cell line, in which the expression of the two NGF receptors, p140^(trk) (Trk) and p75^(LNGFR) (p75), has been independently manipulated. (caltech.edu)
MAH cells expressing both Trk and p75 stop dividing and acquire a mature neuronal morphology more rapidly and with greater efficiency than MAH cells expressing Trk alone. (caltech.edu)
These data provide evidence that p75 can modulate signal transduction through Trk in a neuronal progenitor cell context and that such modulation has functional consequences for the neuronal differentiation pathway induced by NGF. (caltech.edu)
Immunocytochemistry and quantitative real-time polymerase chain reaction analysis demonstrated that neuronal differentiation of hiPSC-derived neuronal precursors was enhanced after treatment with 1-2 μg/ml RA-loaded PCANs. (edu.au)
Retinoic acid induces neuronal differentiation as revealed by the increased expression of MAP2, the decreased cell doubling rate, and the gain in neuronal morphological features and these events are accompanied by the increased expression level of PKC delta and p67(phox), one of the components of NADPH oxidase. (unige.it)
While a few studies have attempted reprogramming of glioblastoma (GBM) cells toward a neuronal fate, this approach has not yet been used to induce differentiation into other lineages and in vivo data on reduction in tumorigenicity are limited. (lu.se)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)

Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
SFE attenuated the RANKL-mediated induction of NF-κB through inhibition of IκBα phosphorylation, which contributed to inhibiting of RANKL-induced osteoclast differentiation through downregulation of NFATc1. (biomedcentral.com)
Pharmaceutical inhibition of osteoclast differentiation is a current therapeutic approach for the treatment of bone loss-related diseases. (biomedcentral.com)
These results suggested that curcumin effectively regulated the differentiation of naïve, TCM, and TEM cells in the peripheral blood to alleviate DSS-induced experimental colitis, which might be related to the inhibition of JAK1/STAT5 signaling activity. (sciencegate.app)
Pharmacological or genetic inhibition of ERK or JNK suppressed PMA-triggered nuclear translocation of TFEB and FOXO1/3, ATG expression, dissociation of pro-autophagic beclin-1 from its inhibitor BCL2, autophagy induction, and differentiation of HL-60 cells into macrophage-like cells. (ac.rs)
Pharmacological or genetic inhibition of autophagy also blocked PMA-induced macrophage differentiation of HL-60 cells. (ac.rs)
Still other observations demonstrated that light‐GABA pairings that simulate stimuli presented during Pavlovian conditioning cause prolonged elevatiori of intracellular Ca 2+ and transformation of GABA‐induced synaptic inhibition into excitation. (elsevierpure.com)
Some studies involving laboratory animals have shown promising results, such as tumor regression, inhibition of cancer cell growth, and improved survival rates. (medicalnewstoday.com)
For TNFa induced inhibition of myoblast differentiation myogenin expression was reduced. (cdc.gov)
Publisher Correction: RAGE inhibition blunts insulin-induced oncogenic signals in breast cancer. (medscape.com)

The present study investigates the effects of nSP on osteoclast differentiation and the relationship between particle size and these effects. (springer.com)
Collectively, the results suggested that nanosilica exerts no effect on RANKL-induced osteoclast differentiation of RAW264.7 cells, although a detailed mechanistic examination of the nSP70-mediated cytotoxic effect is needed. (springer.com)
Our studies revealed that nSP induced high ROS and pro-inflammatory cytokine production, and these cellular responses may induce excess osteoclast differentiation. (springer.com)
Therefore, we consider that it is necessary to estimate nSP-induced effects on osteoclast differentiation. (springer.com)
Furthermore, because we indicated previously that nSP induced different cellular responses from submicron-sized silica particles, such as those described above, it was of interest to analyse the effects of particle size on osteoclast differentiation. (springer.com)
In this study, we examined the potential of SF extract (SFE) to inhibit receptor activator of NF-κB ligand (RANKL) -induced osteoclast differentiation in cultured mouse-derived bone marrow macrophages (BMMs). (biomedcentral.com)
Osteoclast differentiation was measured by tartrate-resistant acidic phosphatase (TRAP) staining and the TRAP solution assay. (biomedcentral.com)
Osteoclast differentiation marker genes were analyzed by the quantitative real-time polymerase chain reaction analysis. (biomedcentral.com)
SFE significantly decreased osteoclast differentiation in a dose-dependent manner. (biomedcentral.com)
However, PQQ inhibited the appearance of osteoclasts and prevented the decrease of F4/80 macrophage maturation marker on RANKL-stimulated cells, suggesting a preventive action of PQQ on RANKL-induced osteoclast differentiation. (nih.gov)

Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)
In another example, DIF-1 has been shown to reduce the proliferation of gastric cancer cells via upregulation of the MEK-ERK-dependent pathway. (wikipedia.org)
Mechanical modulation of stem cell proliferation and differentiation indicates a unique therapeutic target to aid in tissue regeneration and repair and may represent the basis of a nonpharmacologic strategy to simultaneously prevent obesity and osteoporosis. (elsevierpure.com)
RA is characterized by synovitis with infiltration of immune cells, synovial hyperplasia that arises via proliferation of synovial cells, such as macrophage-like synoviocytes (MLS) and fibroblast-like synoviocytes (FLS), and angiogenesis in the hyperplastic synovium [ 8 , 9 , 10 ]. (biomedcentral.com)
In vitro TNFa induces proliferation and migration but suppresses differentiation of myoblasts. (cdc.gov)
Furthermore, TNFa induces moderate induction of Pax-7, a mediator of myoblast proliferation. (cdc.gov)

Differentiation of human and murine induced pluripotent stem cells to microglia-like cells. (alzforum.org)
Induced pluripotent stem cells, iPSCs, are mature, differentiated cells, such as skin cells, that are reprogrammed in the laboratory to be similar to undifferentiated embryonic stem cells. (jove.com)
These cells are also pluripotent like embryonic stem cells-able to produce all cell types-and are therefore called induced pluripotent stem cells (iPSCs). (jove.com)
In previous studies, induced pluripotent stem cells (iPSCs) differentiated into imDCs, and sinomenine (SN) was used to inhibit the maturation of imDCs. (newswise.com)
This study focused on exploring sinomenine (SN) to promote differentiation of induced pluripotent stem cells (iPSCs) into imDCs (SN-iPSCs-imDCs), and found SN-iPSCs-imDCs with worse donor-specific T-cell stimulatory function, and higher regulatory T-cell proliferative function in vitro and in vivo to induce high immune tolerance. (newswise.com)
We aim to develop a cell culture platform to automatically differentiate human induced pluripotent stem cells (hiPSCs) for lab-on-a chip applications. (dim-elicit.fr)
Human induced pluripotent stem (iPS) cells can differentiate into somatic cells ( 1 ). (spandidos-publications.com)
To address these questions, particularly in a human-relevant system, we have focused upon the use of human induced pluripotent stem cells (iPSCs) to assess their ability to give rise to RGCs, with a particular emphasis upon ipRGC differentiation. (indianactsi.org)
We report thermo-responsive retinoic acid (RA)-loaded poly(N-isopropylacrylamide)-co-acrylamide (PNIPAM-co-Am) nanoparticles for directing human induced pluripotent stem cell (hiPSC) fate. (edu.au)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
The implementation of human pluripotent stem cells (hiPSCs) in cell therapy has an extraordinary potential but faces many practical challenges, including costs associated with growth media and factors. (authorea.com)
Abstract Background Cardiac differentiation of human-induced pluripotent stem (hiPS) cells consistently produces a mixed population of cardiomyocytes and non-cardiac cell types, even when using well-characterized protocols. (figshare.com)
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Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for hyaline cartilage regeneration. (mdc-berlin.de)
Heterogeneous gene expressions of cells are widely observed in self-renewing pluripotent stem cells, suggesting possible coexistence of multiple cellular states with distinct characteristics. (biomedcentral.com)
These results suggest the co-existence of two cellular "sub-states" within the pluripotent stem cell state: high-Nanog sub-state and low-Nanog sub-state, rather than one well-defined, homogenous pluripotent cellular state. (biomedcentral.com)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)

However, scientists have found a way to reprogram these mature cells so that they "de-differentiate" and return to an unspecialized, proliferative state. (jove.com)
In this study, mouse iPSCs were induced to differentiate into imDCs in culture medium without or with SN (iPSCs-imDCs and SN-iPSCs-imDCs). (newswise.com)
Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
Cells from embryonal carcinoma (EC) lines 6050AJ and PCC4.aza 1R differentiate in response to treatment with sodium butyrate as well as retinoic acid (RA) or hexamethylenebisacetamide (HMBA). (rupress.org)
In addition, like ESCs, iPS cells possess indefinite proliferative capacity in vitro, and have been shown to differentiate into hematopoietic cells. (psu.edu)
In vitro studies confirmed that anti-PS re-polarized TAMs from an M2 to M1-like phenotype and drove MDSCs to differentiate into M1-like macrophages and functional dendritic cells. (bmj.com)
We investigated the effect of 5-azacytidine (5-aza) on P19 cells to differentiate into a high-efficacy cardiomyocytes. (korea.ac.kr)

Chronic myeloid leukemia (CML) results from BCR-ABL oncogene, which blocks CML cells differentiation and protects these cells from apoptosis. (jcancer.org)
It is known that less-differentiated and central-memory-like (termed highly reactive) CTLs are the optimal population for ACT-based immunotherapy, because these CTLs have a high proliferative potential, are less prone to apoptosis than more differentiated cells and have a higher ability to respond to homeostatic cytokines 2-7 . (psu.edu)
Colchicine, nocodazole and taxol were found to induce differentiation and apoptosis in HT29 cells. (tau.ac.il)
Apoptosis was characterized by detachment of round cells from the cell layer, condensation of nuclear DNA and annexin V binding to cell surfaces. (tau.ac.il)
Our data indicate that microtubule-interfering agents may serve as an important tool in the study of differentiation and apoptosis in intestinal carcinoma. (tau.ac.il)
More than 15 years ago, cholesterol decrement was first shown to inhibit tumor cell growth, metastasis of tumor cells, and induction of apoptosis (7). (cdc.gov)
RHAMM regulates MMTV-PyMT-induced lung metastasis by connecting STING-dependent DNA damage sensing to interferon/STAT1 pro-apoptosis signaling. (medscape.com)

Externalized PS interacts with immune cells where it actively promotes immunosuppression and tumor progression by expansion of myeloid derived suppressor cells (MDSCs) and M2-like tumor associated macrophages (TAMs). (bmj.com)
Author Correction: Conditioned media of pancreatic cancer cells and pancreatic stellate cells induce myeloid-derived suppressor cells differentiation and lymphocytes suppression. (bvsalud.org)

The increase in ALP, mineral deposition, and osteoblastic genes induced by the mechanical stretch-EPO combination was inhibited by U0126, an ERK1/2 inhibitor. (hindawi.com)
Then, typically, genes for transcription factors, are delivered by viral vectors into the cell nuclei, where they are incorporated into the genome. (jove.com)
The transcription factors then turn on genes that are expressed by embryonic stem cells. (jove.com)
Then, genes for multiple transcription factors are delivered into the cells using a viral vector, and the transcription factor proteins are expressed using the cell's machinery. (jove.com)
The transcription factors then turn on many other genes that are expressed by embryonic stem cells, returning the cells to an undifferentiated, proliferative, and pluripotent state. (jove.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
We thus wondered how cells grow spatially during differentiation, so we labeled cells by expression of marker genes and found that cells expressing the same marker tended to occur in patches. (figshare.com)
PMA also increased nuclear translocation of autophagy transcription factors TFEB, FOXO1, and FOXO3, as well as the expression of several autophagy-related (ATG) genes in HL-60 cells. (ac.rs)
In terms of the gene regulatory network defining cellular potency, Nanog has direct mutual interactions with two other core stem cell specific genes Oct4 and Sox2. (biomedcentral.com)
Also, under/over-expression studies of the master regulator Oct4 have revealed that some self-renewal/pluripotency as well as differentiation genes are expressed in a biphasic manner with respect to the concentration of Oct4. (lu.se)
The model also predicts that reprogramming the network from a differentiated state, in particular the endoderm state, into a stem cell state, is best achieved by over-expressing Nanog, rather than by suppression of differentiation genes such as Gata-6. (lu.se)
TFs being expressed and the downstream differentiation target been established as candidates to regulate both pluripotency and genes being shut off [11]. (lu.se)

These UCNPs allow programmable activation of two synergistic light-gated ion channels VChR1 and Jaws in the same cell to manipulate membrane polarization, demonstrated here for cardiac pacing. (nature.com)
The P19 embryonal carcinoma cell line is a useful model cells for studies on cardiac differentiation. (korea.ac.kr)
In response to 1 μM of 5-aza, P19 cells expressed bone morphogenetic protein-2 (BMP-2), BMP-4, Bmpr1a and Smad1 at day 6 or 9, and also cardiac markers such as GATA-4, Nkx2.5, cardiac troponin I, and desmin were up-regulated in a time-dependent manner after induction of BMP signaling molecules. (korea.ac.kr)
These results suggest that 5-aza treatment could be used as a new method for cardiac differentiation in P19 cells. (korea.ac.kr)
Cardiac progenitor cells (CPCs), migrating from heart tissue, in culture aggregate to form cardiospheres (CSs) in which replication and cardiogenic differentiation occur. (unimib.it)
The aim of our study is to quantify differentiation of a muscle-type Ca(2+) release mechanism in CS-derived cells, correlate it with cardiac differentiation markers and test its dependency on CS formation. (unimib.it)
Expression of non-cardiac lineage was low in general, and similar between Pre- and Post-CS cells. (unimib.it)

The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Moreover, we hypothesized that the high glucose, pyruvate and oxygen concentrations found in typical growth media could inhibit the differentiation of certain lineages. (authorea.com)
However, current approaches for chondrogenic differentiation of hiPSCs are complicated and inefficient primarily due to intermediate embryoid body formation, which is required to generate endodermal, ectodermal, and mesodermal cell lineages. (mdc-berlin.de)

The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo. (scielo.br)
Here, we present methods for the generation of T lymphocytes from iPS cells in vitro, and in vivo programming of antigen-specific CTLs from iPS cells for promoting cancer immune surveillance. (psu.edu)
In vitro and in vivo results implied that Cbfa1-overexpressing ASCs may be an alternative cell source for BMSCs for bone tissue engineering. (jarde.jp)

The latter observations suggest that sodium butyrate has the ability to reactivate a silent cRABP gene in PCC4(RA)-1 cells and thereby lead to extensive differentiation via the retinoid pathway when RA is added. (rupress.org)
Our findings unravel a new pathway orchestrating CD4 + T-cell differentiation and demonstrate that NAD + may serve as a powerful therapeutic agent for the treatment of autoimmune and other diseases. (nature.com)

The undifferentiated murine neuroblastoma Neuro2a cell line (ATCC) was stably transfected with AhR cDNA and the established cell line was named N2a-Rα. (biomedcentral.com)
Sodium butyrate induces histone hyperacetylation and differentiation of murine embryonal carcinoma cells. (rupress.org)
Murine 6050AJ EC cells exposed to sodium butyrate possess hyperacetylated forms of histones H4 and altered forms of histones H2a and H2b, whereas histones from cells treated with other inducers appear to be unaffected. (rupress.org)
Here we show that treatment of PC3 tumor-bearing mice with 2aG4, a murine-version of bavituximab, significantly depleted M2-likeTAMs and MDSCs and increased the presence of M1-like TAMs and mature dendritic cells. (bmj.com)

However, tartrate-resistant acid phosphatase (TRAP) activity and the number of osteoclast cells (TRAP-positive multinucleated cells) were not changed by nSP treatment in the presence of receptor activator of nuclear factor κB ligand (RANKL) at doses that did not induce cytotoxicity by silica particles. (springer.com)
CYP1A1, a typical XRE-regulated gene, mRNA was induced without the application of ligand to the culture medium. (biomedcentral.com)
Stimulation in vitro with a Notch ligand drives T cell differentiation from iPS cells, and TCR gene transduction results in iPS cells differentiating into antigen-specific T cells in vivo, which prevents tumor growth. (psu.edu)
The effect of pyrroloquinoline quinine (PQQ) on receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast formation was examined using RAW 264.7 macrophage-like cells. (nih.gov)

For instance, DIF-like molecules have been shown to inhibit cell growth and bring about cell death through uncoupling in mitochondria. (wikipedia.org)
Using DPI to inhibit NOX activity we show that retinoic acid acts through this enzyme to induce morphological changes linked to the differentiation. (unige.it)
Moreover, using rottlerin to inhibit PKC delta or transfection experiments to overexpress it, we show that retinoic acid acts through this enzyme to induce MAP2 expression and to increase p67(phox) membrane translocation leading to NADPH oxidase activation. (unige.it)
Studies have suggested that statins may inhibit tumor cell growth and possibly prevent carcinogenesis. (cdc.gov)

Abstract: Abstract: Previous observations have implicated GABA as a neurotransmitter released by the vestibular sensory neurons („hair cells") of the snail Hermissenda onto visual sensory neurons, the type B cells, whose cell bodies are the sites of biophysical and biochemical changes during and following Pavlovian conditioning. (elsevierpure.com)

Protocols for hepatocyte differentiation from iPS cells have been studied. (spandidos-publications.com)
Cells were grown in a directed, stepwise manner following established protocols. (indianactsi.org)
In recent years reprogramming techniques, differentiation protocols, and sequencing analysis have opened a field for generating and studying human-derived neurons with greater potential than ever before. (lu.se)
Following the establishment of successful conversion protocols, we then introduce a 3D model for direct conversion that replicates the cell-cell interactions pivotal for physiological relevance, leading to an accelerated induction of functional neurons (Paper III). (lu.se)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)

Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
Follicular helper T cells (Tfh) regulate the differentiation of germinal center B cells and maintain humoral immunity. (sciencegate.app)

These results might indicate that the mechanism by which sodium butyrate promotes differentiation of EC cells is different from the ways in which RA and HMBA act. (rupress.org)

Elimination of tumor cells in the enterocyte lineage allows stem cell-like tumor cells to grow but suppresses invasiveness and reshapes various phenotypes associated with cachexia-like wasting by altering the expression of tumor-derived factors. (biorxiv.org)
Using male C57BL/6J mice, the ability of LMMS (0.2g, 90-Hz signal applied for 15 min/d, 5 d/wk) to simultaneously promote bone formation and prevent diet-induced obesity was correlated to mechanical influences on the molecular environment of the bone marrow, as indicated by the population dynamics and lineage commitment of MSCs. (elsevierpure.com)
In particular, an excess of Cdx2 over Oct4 determines the trophectoderm lineage whereas an excess of Gata-6 over Nanog determines differentiation into the endoderm lineage. (lu.se)
Citation: Chickarmane V, Peterson C (2008) A Computational Model for Understanding Stem Cell, Trophectoderm and Endoderm Lineage Determination. (lu.se)
Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)

There is an immediate need to establish an optimized culture platform to direct the differentiation of hiPSCs into germ layers in a defined nutritional microenvironment to generate cost-effective and robust therapeutics. (authorea.com)
The aim of this study was to identify the optimal nutritional environment by mimicking the in vivo concentrations of three key factors (glucose, pyruvate and oxygen) during the spontaneous differentiation of hiPSCs derived from cord blood, which greatly differ from the in vitro expansion and differentiation scenarios. (authorea.com)
A design of experiments (DoE) was used to investigate the interaction between these three variables during the spontaneous differentiation of hiPSCs. (authorea.com)
Our findings present a novel approach for efficient directed differentiation of hiPSCs through the manipulation of media components whilst simultaneously avoiding the usage of growth factors thus reducing costs. (authorea.com)
We report a new, straightforward and highly efficient approach for chondrogenic differentiation of hiPSCs, which avoids embryoid body formation. (mdc-berlin.de)

Specifically, hydrostatic pressure (HP) has been implicated as a key regulator of AMSC differentiation, whereas the mechanisms that underlie the effects of HP on osteogenesis of AMSCs are not fully understood. (aging-us.com)

In the present study, we used SH-SY5Y neuroblastoma cells to analyse the role of NADPH oxidase in retinoic acid (RA)-induced differentiation, pointing out the involvement of protein kinase C (PKC) delta in the activation of NOX. (unige.it)

Our results revealed that nSP exerted higher cytotoxicity in macrophage cells compared with submicron-sized silica particles. (springer.com)
MAP kinase-dependent autophagy controls phorbol myristate acetate-induced macrophage differentiation of HL-60 leukemia cells. (ac.rs)
PMA-triggered differentiation of HL-60 cells into macrophage-like cells was confirmed by cell-cycle arrest accompanied by elevated expression of macrophage markers CD11b, CD13, CD14, CD45, EGR1, CSF1R, and IL-8. (ac.rs)
Therefore, MAP kinases ERK and JNK control PMA-induced macrophage differentiation of HL-60 leukemia cells through AMPK/mTORC1-independent, TFEB/FOXO-mediated transcriptional and beclin-1-dependent post-translational activation of autophagy. (ac.rs)
Mandic M, Misirkic Marjanovic M, Vucicevic L, Jovanovic M, Bosnjak M, Perovic V, Ristic B, Ciric D, Harhaji-Trajkovic L, Trajkovic V. MAP kinase-dependent autophagy controls phorbol myristate acetate-induced macrophage differentiation of HL-60 leukemia cells. (ac.rs)

We show that NAD + regulates CD4 + T-cell differentiation through tryptophan hydroxylase-1 ( Tph1 ), independently of well-established transcription factors. (nature.com)

Immature dendritic cells (imDCs) play an important role in the induction of donor-specific transplant immunotolerance. (newswise.com)
Immature dendritic cells (imDCs) play an important role in the induction of donor-specific transplant immune tolerance. (newswise.com)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)

Bone morphogenetic proteins (BMP), known as active components of odontogenic differentiation and tooth development, have been used as a growth factor in an appropriate medium. (scielo.br)
Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)

These methods have been previously used to obtain hepatocytes from iPS cells. (spandidos-publications.com)
Therefore, methods for obtaining hepatocytes from iPS cells should be further investigated. (spandidos-publications.com)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
The invention also includes cells, cell lines, testing models and culture systems used in the methods of the present invention and differentiated cells produced therefrom. (justia.com)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
CD117 by immunohistochemical methods sion molecules, involved in cell-cell and in order to clarify the role of the infiltrating cell matrix interactions and thought to take inflammatory cells in the pathomechanisms part in cell motility [ 2,3 ]. (who.int)

Erythropoietin is a glycoprotein hormone that stimulates red blood cell (RBC) production in bone marrow via binding to the cell-surface receptor on hematopoietic progenitor cells, and it has been widely used for treating anemia [ 13 ]. (hindawi.com)
They have a higher odontogenic differentiation capacity compared to other cell groups due to their content of specific progenitor cells. (scielo.br)
The first part of the thesis (Paper I and II) explores reprogramming of adult human fibroblasts and human glial progenitor cells into GABAergic interneurons in 2D cultures. (lu.se)
It is an erythropoiesis-stimulating protein closely related to erythropoietin, a primary growth factor produced in kidney that stimulates the development of erythroid progenitor cells. (medscape.com)

DNA array or real-time PCR techniques were used to evaluate gene expression induced by TNFa in myoblast cultures. (cdc.gov)
Myoblasts from TNFR1 and TNFR2 deficient mice demonstrated that TNFa induced gene expression in primary myoblasts is dependent on both receptors. (cdc.gov)

An increase in chemotaxis can be related to malignant migration of cancer cells. (wikipedia.org)

An odontogenic medium modified by the addition of recombinant human bone morphogenetic protein 2 was used in the experimental groups to induce differentiation. (scielo.br)
Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells. (scielo.br)
Hard tissue formation in a porous HA/TCP ceramic scaffold loaded with stromal cells derived from dental pulp and bone marrow. (scielo.br)
Osteoclasts are specialized as the only bone-resorbing cell type and increased numbers are implicated in the development of bone loss-accompanied diseases such as osteoporosis, periodontitis, rheumatoid arthritis, osteosarcoma, and bone cancer metastases [ 2 - 4 ]. (biomedcentral.com)
Genetic ablation of Batf also inhibited CIA-induced synovitis, synovial hyperplasia, angiogenesis in synovial tissues, pannus formation, bone erosion, and cartilage destruction. (biomedcentral.com)
induces reticulocyte release from bone marrow into blood stream. (medscape.com)
Study design: Osteoinduction in the presence or absence of Saos-2 and/or rhBMP-4 was evaluated in cultured human bone marrow cells. (bvsalud.org)
Results: Bone marrow cells were adherent since the first day of culture and were positive for osteonectin. (bvsalud.org)
Osteoblasts were obtained from human bone marrow cells even in the absence of growth factors and showed a typical morphology. (bvsalud.org)

Together with previous reports indicating that ASK1 functions as a pro-apoptotic signaling intermediate, these results suggest that ASK1 has a broad range of biological activities depending on cell types and/or cellular context. (nih.gov)
Accordingly, there is a compelling need to clarify the biological and cellular responses induced by different particle sizes. (springer.com)
Due to particle shrinkage caused by this thermo-responsive property of PCANs, RA could be released from nanoparticles in the cells upon cellular uptake. (edu.au)
While there is no full understanding of their exact mechanisms of action, proponents hypothesize that antineoplastons restore normal cellular functions and balance, promoting the body's natural defense against cancer cells. (medicalnewstoday.com)
Direct cellular reprogramming has recently gained attention of cancer researchers for the possibility to convert undifferentiated cancer cells into more differentiated, postmitotic cell types. (lu.se)
Here, we employ cellular reprogramming to induce astrocytic differentiation as a therapeutic approach in GBM. (lu.se)
Although older chemotherapeutic drugs are often toxic to normal cells, advances in genetics and cellular and molecular biology have led to development of more selective drugs. (msdmanuals.com)

Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)
Although the use of hematopoietic stem cells (HSCs) for therapeutic purposes has been widely applied in clinic 11-13 , HSCs have reduced differentiation and proliferative capacities, and HSCs are difficult to expand in in vitro cell culture 14-16 . (psu.edu)

The role of reactive oxygen species (ROS) in the regulation of signal transduction processes has been well established in many cell types and recently the fine tuning of redox signalling in neurons received increasing attention. (unige.it)
Together these papers encompass a collection of ways to derive human subtype- specific neurons in vitro and serve as platforms for both reprogramming and differentiation studies. (lu.se)

This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. (scielo.br)
Dental pulp stem cells (DPSC) seem to be the most appropriate cell group for the regenerative approach. (scielo.br)
Dental pulp stem cells: state of the art and suggestions for a true translation of research into therapy. (scielo.br)

The effects of iPSCs-imDCs and SN-iPSCs-imDCs on T-cell stimulatory function, and regulatory T (Treg) cell proliferative function in vitro were analyzed by mixed lymphocyte reaction. (newswise.com)
Differentiated cells express typical glial markers and show a marked decrease in their proliferative state. (lu.se)

Although stem-cell-like tumor cells are well-studied, the roles of tumor cells undergoing differentiation in inducing the phenotypes associated with advanced cancers remains to be elucidated. (biorxiv.org)
Here, we employ Drosophila genetics to demonstrate that the native differentiation program of intestinal stem cells plays a key role in determining an intestinal tumor's capacity to invade and induce various non-tumor-autonomous phenotypes. (biorxiv.org)
Our study provides insight into how a native differentiation program determines a tumor's capacity to induce the phenotypes associated with advanced cancers and suggests that manipulating the differentiation programs co-opted in tumors might be a way to treat some complications of cancer, including cachexia. (biorxiv.org)
Although differentiation of naïve CD4 + T cells has been considered to be an irreversible process, accumulating evidence suggests that T cells are much more flexible than initially believed with the ability to interchange between helper phenotypes 15 . (nature.com)

Human embryonic stem cells also exhibit the Warburg effect ( 15 ). (spandidos-publications.com)

However, due to difficulties in obtaining a high number of such CTLs from patients, there is an urgent need to find a new approach to generate highly reactive Ag-specific CTLs for successful ACT-based therapies.TCR transduction of the self-renewable stem cells for immune reconstitution has a therapeutic potential for the treatment of diseases 8-10 . (psu.edu)
CD4 + T cells are involved in the development of autoimmunity, including multiple sclerosis (MS). Here we show that nicotinamide adenine dinucleotide (NAD + ) blocks experimental autoimmune encephalomyelitis (EAE), a mouse model of MS, by inducing immune homeostasis through CD4 + IFNγ + IL-10 + T cells and reverses disease progression by restoring tissue integrity via remyelination and neuroregeneration. (nature.com)
CD4 + T helper (Th) cells play a central role in regulating the adaptive immune response associated with pathogen invasion. (nature.com)
These data suggest that PS expression on the external cell surface defines an upstream immune checkpoint that is primarily responsible for expansion of MDSCs and M2-like TAMs in tumors. (bmj.com)
Immune features in RA were analyzed by examining immune cell populations and cytokine production. (biomedcentral.com)
Synovial cells express numerous cytokines that have been implicated in many of the immune processes involved in RA [ 13 ]. (biomedcentral.com)

By immunostaining for cell type specific markers, I have documented the different stages of retinal differentiation and provided insight into the mechanisms of ipRGC differentiation. (indianactsi.org)
In contrast, regulatory T cells (Tregs) play a cardinal role in protecting against autoimmune diseases, inflammation and tissue damage 13 through secretion of IL-10 (ref. 14 ) and other mechanisms. (nature.com)
This study reported that Nam reduced CD4 + T cell infiltration and demyelination 24 , but failed to identify the protective mechanisms of action. (nature.com)
We investigated the mechanisms and the role of autophagy in the differentiation of HL-60 human acute myeloid leukemia cells induced by protein kinase C (PKC) activator phorbol myristate acetate (PMA). (ac.rs)
Here, we explored the roles and regulatory mechanisms of BATF in collagen-induced arthritis (CIA) in mice. (biomedcentral.com)
However, these diseases clearly differ in their etiologies, pathogenic mechanisms, and the cell types associated with each pathogenesis. (biomedcentral.com)
For chemotherapy drugs, one of the best characterized resistance mechanisms is overexpression of MDR1 , a cell membrane transporter that causes efflux of certain drugs (eg, vinca alkaloids, taxanes, anthracyclines). (msdmanuals.com)

Consequently, PLDL and PDL seemed to be promising scaffold candidates for odontogenic regeneration at least as HA-TCP, when they were applied with the DPSC induced for odontogenic differentiation. (scielo.br)
Although different stem cells, morphogens and scaffolds can be combined to simulate natural odontogenic regeneration, it is crucial to determine which options most closely replicate the characteristics of human dental tissues. (scielo.br)

PS is externalized on tumor vasculature, tumor-derived exosomes, and tumor cells in the tumor microenvironment and externalization is markedly enhanced by therapy (e.g., radiation, chemotherapy, and/or androgen deprivation). (bmj.com)
We take advantage of the similarity between neural developmental processes and cancer hierarchy to mitigate, if not completely abolish, the malignant nature of tumor cells and pave the way for new intervention strategies. (lu.se)

To create iPSCs, mature cells, like skin fibroblasts, are taken from a patient and put into culture. (jove.com)
iPSCs are potentially valuable in medicine, because a patient who needs a particular cell type-for instance, someone with a damaged retina due to macular degeneration-could receive a transplant of the required cells, generated from another cell type in their own body. (jove.com)
To create iPSCs, mature cells such as skin fibroblasts or blood cells from a person are grown in culture. (jove.com)
It is still being studied whether iPSCs are truly equivalent to embryonic stem cells, but they appear to be similar and can produce cells from all three germ layers of the body. (jove.com)
As with other types of stem cells, scientists are learning how to promote the differentiation of specific cell types from iPSCs efficiently, so that the needed cell types can be produced in adequate quantities. (jove.com)
To study the capacity of SN to maintain iPSC-derived imDCs (SN-iPSCs-imDCs) in an immature state and the mechanism by which SN-iPSCs-imDCs induce immunotolerance. (newswise.com)
The induced immunotolerance of SN-iPSCs-DCs was evaluated by treating recipient Balb/c skin graft mice. (newswise.com)
Both iPSCs-imDCs and SN-iPSCs-imDCs were successfully obtained, and their biological characteristics and ability to induce immunotolerance were compared. (newswise.com)
We found that iPSCs can be effectively differentiated into retinal cells, including the formation of 3D retinal organoids. (indianactsi.org)

In addition, forced differentiation induces astrocytic functions such as induced calcium transients and ability to respond to inflammatory stimuli. (lu.se)

culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)

Differentiation-inducing factor (DIF) is one of a class of effector molecules that induce changes in cell chemistry, inhibiting growth and promoting differentiation of cell type. (wikipedia.org)
Significant changes in the percentages of naïve, central memory T (TCM), and effector memory (TEM) cells and their CD4+ and CD8+ subsets were found in the peripheral blood of mice with colitis using flow cytometry. (sciencegate.app)

Adoptive cell transfer (ACT) of antigen-specific CD8 + cytotoxic T lymphocytes (CTLs) is a promising treatment for a variety of malignancies 1 . (psu.edu)
Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)

After two days of culture in HSM, the expression levels of α-fetoprotein (AFP) and albumin, which are hepatocyte markers, were upregulated, suggesting that hepatocyte differentiation was initiated ( 19 ). (spandidos-publications.com)

The excessive activation of AhR resulted in neural differentiation of Neuro2a cells. (biomedcentral.com)

Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
N2a-Rα cells exhibited two significant functional features. (biomedcentral.com)
N2a-Rα cells expressed tyrosine hydroxylase (TH) mRNA as a functional marker of catecholaminergic neurotransmitter production. (biomedcentral.com)
However, the frequency of functional differentiation in CSs and the role of cell clustering in supporting it remain to be established. (unimib.it)
Very few studies have addressed the functional roles of the bimodal heterogeneity of Nanog expression in the differentiation and specification process of stem cells. (biomedcentral.com)
We further explore the contribution of various glial cells in a 3D co-culture setting with interneurons employing both functional and molecular analyses. (lu.se)

He proposed that they work by targeting various biochemical pathways involved in cancer cell growth and survival. (medicalnewstoday.com)

Isolation of 10 differentially expressed cDNAs in differentiated Neuro2a cells induced through controlled expression of the GD3 synthase gene. (nih.gov)

It has been shown that Th17 cells can also co-express Foxp3 or T-bet 18 , 19 , which are the master regulators of Tregs and Th1 cells, respectively. (nature.com)
Batf knockout inhibited the differentiation of T helper (Th)17 cells and the conversion of CD4 + Foxp3 + cells to CD4 + IL-17 + cells. (biomedcentral.com)

The human colon adenocarcinoma cell line HT29 displays an undifferentiated phenotype under standard growth conditions. (tau.ac.il)
Treatment with colchicine or forskolin induced the association of E-cadherin to the cytoskeleton fraction of subconfluent HT29 cells. (tau.ac.il)

Anatomy70

Organisms7

Diseases7

Chemicals and Drugs87

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes57

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science3

Osteogenic20

Mesenchymal7

Neuronal8

Inhibition10

Osteoclast differentiation10

Proliferation8

Pluripotent stem22

Differentiate9

Apoptosis7

Myeloid derived suppressor cells2

Genes14

Cardiac7

Lineages3

Vitro and in vivo3

Pathway2

Murine4

Ligand4

Inhibit4

Abstract1

Protocols7

Regulate the differentiation2

Promotes1

Lineage5

HiPSCs5

Osteogenesis1

Neuroblastoma cells1

Macrophage5

Regulates1

Induction4

Proteins2

Methods8

Progenitor cells4

Gene Expression2

Migration1

Bone9

Cellular7

Hematopoietic2

Neurons2

Dental pulp stem cells3

Proliferative2

Phenotypes4

Human Embryonic Stem1

Immune6

Mechanisms7

Regeneration2

Tumor cells2

IPSCs9

Transients1

Embryonic cell1

Effector2

Lymphocytes2

Markers1

Neural1

Functional6

Biochemical1

Neuro2a1

FoxP32

HT292