• Thus, unlike other tumor suppressors, RPL5/RPL11 play an essential role in normal cell proliferation, a function cells have evolved to rely on in lieu of a cell cycle checkpoint. (nih.gov)
  • Functional analysis revealed that knockdown of PTGR1 significantly inhibited proliferation and colony formation by PC cells. (go.jp)
  • The inhibition of cell proliferation was related to arrest of the cell cycle in the G0/G1 phase and increased apoptosis in response to PTGR1 knockdown as indicated by flow cytometry. (go.jp)
  • Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
  • Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
  • The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
  • 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced on VALD-3. (researchsquare.com)
  • Our study demonstrates that survivin and APE1/Ref-1 are significantly higher in human prostate cancer specimens compared to noncancerous controls and that APE1/Ref-1 redox-specific inhibition with small molecule inhibitor, APX3330 and a second-generation inhibitor, APX2009, decreases prostate cancer cell proliferation and induces cell cycle arrest. (oncotarget.com)
  • The current study demonstrated that PD markedly inhibited PAC cell proliferation and triggered their apoptosis. (biomedcentral.com)
  • Dichloroacetate (20 mM) did not reduce growth of non-cancerous cells but caused significant decrease in cancer cell proliferation ( P =0.009), which was associated with apoptosis and G 2 phase cell-cycle arrest. (dcaguide.org)
  • Glucose-6 phosphatase catalytic subunit inhibits the proliferation of liver cancer cells by inducing cell cycle arrest]. (bvsalud.org)
  • To investigate the effects of glucose-6-phosphatase catalytic subunit (G6PC) recombinant adenovirus on proliferation and cell cycle regulation of liver cancer cells . (bvsalud.org)
  • Cell proliferation and clone formation assay were used to observe the proliferation of liver cancer cells . (bvsalud.org)
  • Cell cycle flow cytometry assay was used to analyze the effect of G6PC overexpression on the proliferation cycle of liver cancer cells . (bvsalud.org)
  • Huh7 and SK-Hep1 cells proliferation assay showed that the number of proliferating cells in the AdG6PC group was significantly lower than the other two groups (P (bvsalud.org)
  • Western blot result showed that G6PC overexpression had down-regulated the proliferation in cell -cycle related proteins expression. (bvsalud.org)
  • G6PC inhibits the proliferation, cell -cycle related expression, and migration of liver cancer cells by inhibiting the G(1)/ S phase transition. (bvsalud.org)
  • Apoptotic death is preceded by proliferation blockage, suggesting that these transcripts are involved in cell cycle regulation. (uandes.cl)
  • Our results suggest that ASncmtRNA targeting blocks tumor cell proliferation through reduction of essential cell cycle proteins, mediated by mitochondrial and nuclear miRNAs. (uandes.cl)
  • Interestingly, compared with A549 cells, C1632 possesses the exact same and even better anti-migration and anti-proliferation effects on A549R cells, regardless of drug resistance. (cathepsin-s.com)
  • On the other hand, small molecule tyrosine kinase inhibitors could only be utilized to get a little minority of NSCLC sufferers with gene alterations.15 Consequently, the all round remedy and survival prices of NSCLC remain low.1,16 Therefore, continued analysis into new smaller molecule inhibitors that considerably suppress NSCLC cell motility and invasiveness too as proliferation is preferred. (cathepsin-s.com)
  • Open up in another window Shape 2 Leucine deprivation inhibits breasts tumor cell proliferation(A) MDA-MB-231 or CORIN MCF-7 cells had been seeded in 12-well plates and incubated in charge (+leu) or leucine-deficient (?leu) moderate. (racetab.org)
  • Protein transport between the nuclear and the cytoplasmic compartment is a key factor for cell viability and proliferation. (massgeneral.org)
  • At a fundamental level, cancer is caused by an accumulation of genetic changes that result in unregulated cell growth and proliferation. (medscape.com)
  • Tumor suppressor genes encode proteins that normally provide negative control of cell proliferation. (medscape.com)
  • PTEN encodes a protein kinase of the same name and functions as a tumor suppressor through regulation of cell proliferation. (medscape.com)
  • The aim of this study is to investigate potential synergistic effects of Flavopiridol and Lonfarnib inhibiting the proliferation of human ovarian cancer cells in vitro. (uni-marburg.de)
  • End points are proliferation, cell cycle allocation, induction of apoptosis and necrosis. (uni-marburg.de)
  • The following methods were applied: at first proliferation assays with photometric analysis were conducted, then an annexin-V-detection was applied for differentiation of the treated cells in vital, necrotic and apoptotic groups. (uni-marburg.de)
  • Analysing the results of the proliferation assays synergistic effects when combining Flavorpiridol and Lonafarnib could not be shown in SKOV3-cells but in BG1-cells. (uni-marburg.de)
  • In addition, we studied the effect of WSC on the proliferation and cell cycle of alveolar type II cells and vascular endothelial cells. (who.int)
  • It is an artificial activation of naturally occurring cell cycle checkpoints, induced by exogenous stimuli controlled by an experimenter. (wikipedia.org)
  • Dysfunctional CHK2 impairs DNA damage-induced checkpoints, reduces apoptosis, and confers resistance to chemotherapeutic drugs and radiation therapy in ovarian cancer cells. (tku.edu.tw)
  • These findings establish a mechanism for co-targeting DNA damage-induced cell cycle checkpoints in combination with repair of cisplatin-DNA lesions in vivo using RNAi nanocarriers, and motivate further exploration of ASL as a generalized strategy to improve cancer treatment. (nature.com)
  • While in G1 the cell synthesizes messenger RNA (mRNA) and proteins in preparation for subsequent steps of interphase leading to mitosis. (wikipedia.org)
  • The expression of pro-apoptotic proteins and anti-apoptotic proteins in MCF-7 and MDA-MB-231 cells was investigated by Western blotting. (researchsquare.com)
  • In addition, proteome analysis of NBPF1-overexpressing DLD1 cells identified 32 differentially expressed proteins, of which several are implicated in carcinogenesis. (biomedcentral.com)
  • We demonstrated that NBPF1 exerts different tumor suppressive effects, depending on the cell line analyzed, and provide new clues into the molecular mechanism of the enigmatic NBPF proteins. (biomedcentral.com)
  • Cell cycle and apoptotic regulatory proteins were determined by western blot analysis. (upm.edu.my)
  • Diverse models show that Flavoprirol inhbitis the TNF-mediated NF-κB-activation and the AP-1-activation, suppresses the expression of a multiplicity of antiapoptotic proteins and the TNF-induced AKT-activation. (uni-marburg.de)
  • Although the senescent cells remain viable, they show typical changes with enlarged and flattened cell bodies, apoptosis resistance, increased activity of senescence-associated β -galactosidase (SA- β -gal), and upregulation of cyclin-dependent kinase (CDK) inhibitors including p16 INK4A , ARF proteins, and p21 [ 13 - 16 ]. (hindawi.com)
  • Eukaryotic expression vectors containing genes encoding plant proteins for killing of cancer cells. (weeksmd.com)
  • consequently, genes encoding some of these proteins are being used to design constructs for the inhibition of multiplying cancer cells. (weeksmd.com)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)
  • It specifically negatively regulates the PI3K-AKT signaling pathway to induce cell cycle arrest. (medscape.com)
  • Furthermore, the combined drug effects of TCEE with conventional chemotherapy agents, cisplatin and doxorubicin, were also analyzed to clarify whether TCEE enhances or antagonizes the cytotoxicity of the selected chemotherapy agents in hepatocellular carcinoma cells. (hindawi.com)
  • In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. (mdpi.com)
  • Cell cycle-dependent cytotoxicity, G2/M phase arrest, and disruption of p34cdc2/cyclin B1 activity induced by doxorubicin in synchronized P388 cells. (aspetjournals.org)
  • We studied the effect of doxorubicin (Dox) on cell cycle progression and its correlation with DNA damage and cytotoxicity in p53-mutant P388 cells. (aspetjournals.org)
  • Our results suggest that anthracycline-induced cytotoxicity is cell cycle dependent and is mediated, at least in part, by disturbance of the regulation of p34cdc2/cyclin B1 complex, thus leading to G2/M phase arrest. (aspetjournals.org)
  • Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)
  • In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
  • Andrographolide (AGP), the major phytoconstituent isolated from Andrographis paniculata was found to exhibit growth inhibition and cytotoxicity against the hormone-independent (PC-3 and DU-145) and hormone-dependent (LNCaP) prostate cancer cell lines via the microculture tetrazolium (MTT) assay. (upm.edu.my)
  • BAY 293 We looked into the cytotoxicity of leucine deprivation on regular cells further, including major hepatocytes, brownish adipose cells (BAT) and MCF-10A cells. (racetab.org)
  • Adding lunasin to IL-12- or IL-2-stimulated NK cells demonstrated synergistic effects in the induction of IFNG and GZMB involved in cytotoxicity. (weeksmd.com)
  • Given that some of the mechanisms below of inducing cell cycle arrest involve damaging the DNA, this allows investigation into how the cell responds to damage of its genetic material. (wikipedia.org)
  • The underlying molecular mechanisms by which androgen-independent PC cells acquire the capacity to proliferate remain largely unclear. (go.jp)
  • In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
  • Our aim was to determine if switching metabolism from glycolysis towards mitochondrial respiration would reduce growth preferentially in colorectal cancer cells over normal cells, and to examine the underlying mechanisms. (dcaguide.org)
  • While p21 is usually activated by p53-dependent mechanisms in response to DNA damage to make sure cell cycle arrest and repair a number of realtors that promote differentiation like phorbol ester 67346-49-0 or okadaic acidity can up-regulate p21 separately of p53 (for an assessment see reference point 16). (bio2009.org)
  • This work adds to the elucidation of the molecular mechanisms behind cell cycle arrest preceding tumor cell apoptosis induced by ASncmtRNA knockdown. (uandes.cl)
  • In the present study, we found that knockdown of UCK2 induced cell cycle arrest through dual mechanisms in HCC and that targeting UCK2 promoted the secretory features involved in the senescence-associated secretory phenotype (SASP) and inflammasomes. (biomedcentral.com)
  • The purpose of BAY 293 our current research would be to investigate the result of leucine deprivation on breast tumor cells and also to elucidate the root mechanisms. (racetab.org)
  • In this review, we will discuss the mechanisms according to which T2D induces cellular senescence and the role of type 2 diabetes-induced cellular senescence in the pathogenesis and progression of colorectal cancer. (frontiersin.org)
  • Findings from the Cusack Laboratory have shown that conventional anti-cancer therapies, such as radiation and chemotherapy, induce resistance mechanisms under the control of the gene transcription factor NF-kappaB. (massgeneral.org)
  • To investigate the mechanisms of retinal iron regulation, the lab uses conditional knockout mouse models, human retinal tissue, and retinal cell tissue culture. (upenn.edu)
  • The mechanisms influenced by Flaoprirol and Lonafarnib are relevant on ovarian cancer cells. (uni-marburg.de)
  • The other proposed mechanisms include passage of cell-free virus into the brain and release of the virus from the infected endothelial cells. (medscape.com)
  • Induced cell cycle arrest is the use of a chemical or genetic manipulation to artificially halt progression through the cell cycle. (wikipedia.org)
  • The cells can be induced to arrest as they arrive (at different time points) at a certain phase, so that when the arrest is lifted (for instance, rescuing cell cycle progression by introducing another chemical) all the cells resume cell cycle progression at the same time. (wikipedia.org)
  • Mimosine is a plant amino acid that has been shown to reversibly inhibit progression beyond G1 phase in some human cells, including lymphoblastoid cells. (wikipedia.org)
  • The use of serum deprivation - partially or completely removing the serum and its nutrients - has been shown to arrest and synchronize cell cycle progression in G0 phase, for example in neonatal mammalian astrocytes and human foreskin fibroblasts. (wikipedia.org)
  • Unexpectedly, we found that their depletion in primary human lung fibroblasts failed to induce cell cycle arrest but strongly suppressed cell cycle progression. (nih.gov)
  • We show that the effects on cell cycle progression stemmed from reduced ribosome content and translational capacity, which suppressed the accumulation of cyclins at the translational level. (nih.gov)
  • In untreated control P388 cells, p34cdc2 kinase localizes in the nucleus and cytoplasms, particularly in the centrosomes, and p34cdc2 kinase activity is dependent on cell cycle progression, with the enzyme activity increasing steadily from G1/S to G2/M and markedly declining thereafter. (aspetjournals.org)
  • Cell cycle analysis suggested activation of apoptosis, without arresting cell cycle progression. (nature.com)
  • A novel UCS cell line (PF338) was established from the malignant pleural effusion of a 59-year-old patient at time of disease progression. (por-journal.com)
  • Importantly, UCS is the prototype tumor for epithelial-mesenchymal transition (EMT), a reversible biological process that associates with tumor progression and metastasis and in which epithelial cells transform into more invasive mesenchymal cells by losing their epithelial properties [ 10 - 12 ]. (por-journal.com)
  • This effect is mediated by downregulation of the key cell cycle progression factors cyclin B1, cyclin D1, CDK1, CDK4, and survivin, the latter also constituting an essential inhibitor of apoptosis, underlying additionally the onset of apoptosis. (uandes.cl)
  • UCK2 promoted cell cycle progression of HCC by preventing the degradation of mTOR protein and maintaining the stability of PDPK1 mRNA. (biomedcentral.com)
  • Moreover, the results clearly showed that kaempferol causes a strong inhibition of the activation of the EGFR/p38 signaling pathways, upregulation of p21 expression and downregulation of cyclin B1 expression in human RCC cells, together with activation of PARP cleavages, induction of apoptotic death and inhibition of cell growth. (nih.gov)
  • Modulation of pro and anti-apoptotic genes in cervical cancer cells after exposure to MNBE. (biomedcentral.com)
  • The largest apoptotic effect was evident in metastatic LoVo cells, in which DCA induced up to a ten-fold increase in apoptotic cell counts after 48 h. (dcaguide.org)
  • AGP was found to induce G2/M cell cycle arrest which led to predominantly apoptotic mode of cell death. (upm.edu.my)
  • The existence of apoptotic bodies, cell membrane blebbing and chromatin condensation indicated the hallmark of apoptosis. (um.edu.my)
  • The treatment also induces an increase in the microRNA hsa-miR-4485-3p, whose sequence maps to ASncmtRNA-2 and transfection of MDA-MB-231 cells with a mimic of this miRNA induces cyclin B1 and D1 downregulation. (uandes.cl)
  • Downregulation of UCK2 induced cell cycle arrest and activated the TNFα/NFκB signalling pathway-related senescence-associated secretory phenotype to modify the tumour microenvironment. (biomedcentral.com)
  • During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)
  • Strikingly, histone deacetylase (HDAC) inhibition markedly reduced cell viability by inducing a dose-dependent G0/1 arrest and led to mesenchymal-epithelial transition as evidenced by morphological change and increased E-cadherin and β-catenin expression. (por-journal.com)
  • In addition, pyrimidine metabolites can induce epithelial to mesenchymal transition to promote tumour metastasis, playing a non-proliferative role in pyrimidine metabolism in cancer [ 7 ]. (biomedcentral.com)
  • Towards elucidating the effects of purified MWCNTs on human lung epithelial cells. (cdc.gov)
  • Herein, we examined the potential of purified MWCNTs used at occupationally relevant exposure doses for particles not otherwise regulated to affect human lung epithelial cells. (cdc.gov)
  • Basal cell carcinoma (BCC) is a nonmelanocytic skin cancer (ie, an epithelial tumor) that arises from basal cells (ie, small, round cells found in the lower layer of the epidermis). (medscape.com)
  • Cellular processes like genome duplication and cell division stop. (wikipedia.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Because age-related cellular senescence and type 2 diabetes (T2D) have been recognised as risk factors for CRC development, the recent finding that type 2 diabetic patients present an elevated circulating volume of senescent cells raises the question whether type 2 diabetes facilitates the process of CRC tumorigenesis by inducing premature cell senescence. (frontiersin.org)
  • Senescence is a stress-response cellular state characterised by proliferative arrest but active metabolism ( 7 ). (frontiersin.org)
  • Age-related and metabolic diseases such as type 2 diabetes (T2D) represent a source of cellular stress due to their disruptive effect on normal physiological processes and, therefore, can induce premature senescence ( 10 ). (frontiersin.org)
  • Recent research in the lab indicates that inflammation promotes cellular iron overload in a vicious cycle leading to cell death. (upenn.edu)
  • In this manuscript, we report the in vitro mutagenicity of waterpipe smoke condensate (WSC), the alteration in cellular parameters of lung alveolar cells in response to WSC exposure and discuss the implication of cellular responses in the pathophysiology of chronic obstructive pulmonary disease (COPD). (who.int)
  • However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. (who.int)
  • CONCLUSION: The data reported here represent the first in vitro demonstration of the effect of waterpipe smoke on cellular parameters providing evidence of the potential involvement of WPS in the pathogenesis of COPD through impairing cellular growth and inducing inflammation. (who.int)
  • Our analysis further emphasized that the MWCNTs-induced cellular fate result from multiple types of interactions that could be analyzed by means of intracellular biomechanical changes and are pivotal in understanding the underlying MWCNT-induced cell transformation. (cdc.gov)
  • The term Langerhans cell histiocytosis is generally preferred to the older term, histiocytosis X. This newer name emphasizes the histogenesis of the condition by specifying the type of lesional cell and removes the connotation of the unknown ("X") because its cellular basis has now been clarified. (medscape.com)
  • Specifically, a variety of other cellular populations have been identified that possess phenotypic characteristics similar to Langerhans cells, including expression of CD207 and Birbeck granules. (medscape.com)
  • Therefore, in addition to epidermal Langerhans cells, other potential cellular origins for LCH include dermal langerin + dendritic cells, lymphoid tissue-resident langerin + dendritic cells, and monocytes that can be induced by local environmental stimuli to acquire a Langerhans cell phenotype. (medscape.com)
  • Here, we show that ASncmtRNA knockdown induces cell death preceded by proliferative blockage in three different human breast cancer cell lines. (uandes.cl)
  • Baicalein-induced G2/M phase arrest is associated with an increased reactive oxygen species (ROS) production, DNA damage, and CHK2 upregulation and activation. (tku.edu.tw)
  • They further link to the activation of protein kinase C- (PKC-) induced generation of reactive oxygen species (ROS) [ 6 , 7 ], which further mediates the activation of downstream transcription factor nuclear factor kappa-light-chain enhancer of activated B cells (NF- κ B). Thus, the main treatments of DN refer to modulate glycemic and blood pressure through insulin and RAS inhibitors. (hindawi.com)
  • Genetic engineering of cells with specific gene knockouts can also result in cells that arrest at different phases of the cell cycle. (wikipedia.org)
  • G2: Fission yeast expressing some mutant forms of CDC2 unable to arrest in G2 in response to DNA damage, indicating the gene product is involved in G2 arrest. (wikipedia.org)
  • effects the main target for p53-induced cell cycle arrest seems to be the p21 gene. (bio2009.org)
  • The transcriptional activation from CACNA1F the p21 gene by these inhibitors is normally marketed by chromatin redecorating pursuing acetylation of histones H3 and H4 in the p21 promoter area (32 54 This activation of p21 takes place within a p53-unbiased fashion and for that reason HDAC inhibitors are appealing realtors for cancers therapy being that they are operative in cells with mutated p53 genes a hallmark of several tumors. (bio2009.org)
  • Activation of p53 efficiently counteracts deacetylase-mediated repression and induces cell cycle arrest by activating the p21 gene. (bio2009.org)
  • In UCS, TP53 has been identified as the most frequently mutated gene, followed by mutations in the PI3K pathway, KRAS , cell cycle regulators including FBXW7 and chromatin remodeling and histone genes including ARID1A [ 7 - 9 ]. (por-journal.com)
  • Reprimo (RPRM), a downstream effector of p53-induced cell cycle arrest at G2/M, has been proposed as a putative tumor suppressor gene (TSG) and as a potential biomarker for non-invasive detection of gastric cancer (GC). (pucv.cl)
  • The aim of this study was to evaluate the epigenetic silencing of RPRM gene by promoter methylation and its tumor suppressor function in GC cell lines. (pucv.cl)
  • Epigenetic silencing of RPRM gene by promoter methylation was evaluated in four GC cell lines. (pucv.cl)
  • The researchers demonstrated that Dmp1 also showed haploid insufficiency, meaning that cancer developed even when one copy of the gene was present, in samples from lung cancer patients with non-small-cell lung cancers. (eurekalert.org)
  • We have demonstrated that the hDMP1 gene is hemizygously deleted in a significant percentage of mouse and human non-small-cell lung carcinomas, especially those which retained the intact Arf-p53 pathway," explains Dr. Inoue. (eurekalert.org)
  • Since hDMP1 loss-of-heterozygosity lung cancer cells retain one allele of the hDMP1 locus, this gene might be a promising target for future drug development. (eurekalert.org)
  • SL originally described a relationship between two genes, where alteration of either gene alone results in viable cells, but alteration (mutation, loss, or inhibition) of both genes simultaneously was lethal. (nature.com)
  • The underlying mechanism responsible for the effects of lunasin on NK cells is likely due to epigenetic modulation on target gene loci. (weeksmd.com)
  • Gene therapy has attracted attention for its potential to specifically and efficiently target cancer cells with minimal toxicity to normal cells. (weeksmd.com)
  • immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
  • M: A mutant screen of budding yeasts with mitotic arrest identified CDC16, CDC23, and CDC27 as key genes that, when mutated, cause arrest in mitosis. (wikipedia.org)
  • We investigated by real-time quantitative RT-PCR the expression profile of a panel of genes important in cell cycle regulation. (biomedcentral.com)
  • Because BRCA mutations are observed in fewer than 10% of cancer patients (cBioPortal: 6.7%) 11 , 12 , 13 the identification of additional genes that share synthetic lethal sensitivity relationships with mutated oncogenes or tumor suppressors would greatly enhance the implementation of tumor cell-specific synthetic lethal sensitivity to improve an anticancer therapeutic response. (nature.com)
  • We also assessed the effect of WSC on the expression of genes involved in cell cycle arrest and inflammation. (who.int)
  • Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
  • An herbal formula inhibits STAT3 signalling and attenuates bone erosion in collagen-induced arthritis. (greenmedinfo.com)
  • Here, the results show that baicalein (5,6,7-trihydroxyflavone) treatment inhibits the growth of highly invasive ovarian cancer cells, and that baicalein-induced growth inhibition is mediated by the cell cycle arrest in the G2/M phase. (tku.edu.tw)
  • Our findings showed that plagioneurin B successfully inhibits the growth of CAOV-3 cells at IC 50 of 0.62 µM. (um.edu.my)
  • Lonafarnib inhibits the farnesylation of H-Ras and the attachment of Ras to the cell membrane. (uni-marburg.de)
  • In this model, 16e was shown to abrogate topotecan-induced cell cycle arrest in a dose dependent manner. (rcsb.org)
  • In-vitro-treatment with Lonafarnib leads to a dose-dependent G2-arrest of glioblastomacells, lungcancer- and fibrosarcomacells. (uni-marburg.de)
  • The glycolytic phenotype offers growth advantages to cancer cells by resisting apoptosis, and facilitating tumour spread and metastasis (Yeluri et al, 2009). (dcaguide.org)
  • Downregulated UCK2 induced a secretory phenotype, which could improve the microenvironment, and decreased UCK2 remodelling metabolism could lower the resistance of tumour cells to T-cell-mediated killing. (biomedcentral.com)
  • Furthermore, senescent cells, with the secretory features known as the senescence-associated secretory phenotype (SASP), could produce proinflammatory cytokines, such as tumor necrosis factor- α (TNF- α ), interleukin-6 (IL-6), and monocyte chemoattractant protein1 (MCP-1), to greatly affect the neighboring cells [ 17 , 18 ]. (hindawi.com)
  • introduced a new concept of CKD-associated secretory phenotype (CASP), which indicates that senescent renal cells could secrete SASP components of various cytokines such as IL-1, IL-6, and TNF- α [ 19 , 20 ]. (hindawi.com)
  • The aims of this study are to clarify the antitumor effects of TCEE on human hepatocellular carcinoma cells and also evaluate the combination drug effects with conventional chemotherapy agents, cisplatin and doxorubicin. (hindawi.com)
  • In contrast, the antitumor effects and related biological mechanism of TCEE as well as the combination drug effects with conventional chemotherapy agents remain unclear particularly in human hepatocellular carcinoma cells. (hindawi.com)
  • Because most tumors are deficient in one or more aspects of the function of the p53 tumor suppressor, either as a consequence of mutations within p53, or impairment of upstream and downstream modulators of p53 activity 19 , targeting MK2 has the potential to selectively enhance tumor cell killing without increasing the genotoxic effects of chemotherapy on normal p53-wild type tissues. (nature.com)
  • It has been reported to reduce growth of these cancer cells mainly by reducing inhibitory phosphorylation of PDH, thereby promoting mitochondrial oxidative phosphorylation and inducing apoptosis through mitochondrial, NFAT-Kv 1.5, and p53 upregulated modulator of apoptosis (PUMA)-mediated pathways. (dcaguide.org)
  • All the results suggest that baicalein has another novel growth inhibitory effect on highly invasive ovarian cancer cells, which is partly related to G2/M cell cycle arrest through the ROS-mediated DNA breakage damage and CHK2 activation. (tku.edu.tw)
  • As there is a substantial growth-inhibitory aftereffect of leucine deprivation, we explored whether leucine deprivation got any inhibitory influence on cell routine development. (racetab.org)
  • In fact, several studies have shown that T2D induces senescence in multiple types of cells, including fibroblasts and endothelial cells ( 11 , 12 ). (frontiersin.org)
  • Microparticles Induce Cell Cycle Arrest Through Redox-Sensitive Processes in Endothelial Cells: Implications in Vascular Senescence. (ohri.ca)
  • Expression of cell cycle inhibitors, P21 and P27, and activation of apoptosis executer enzyme, caspase-3, were also induced by TCEE. (hindawi.com)
  • Two PTGR1-specific short hairpin RNA (shRNA) sequences were used to block the expression of PTGR1 via a lentivirus-mediated system in the androgen-independent PC cell lines DU145 and PC 3. (go.jp)
  • We show here that expression of EBF3 resulted in cell cycle arrest and apoptosis. (aacrjournals.org)
  • In this study, we further investigated the mechanism of PA-induced p27 expression in prostate cancer cells (LNCaP, androgen-independent LNCaP [AIDL] and PC-3). (iiarjournals.org)
  • A striking decrease in Skp2 mRNA and protein expression and reciprocal increase in p27 protein level were observed in three PA-treated prostate cancer cells. (iiarjournals.org)
  • Our results suggest that PA attenuated Skp2 expression, thereby inhibiting ubiquitination and promoting p27 accumulation in all three prostate cancer cell lines. (iiarjournals.org)
  • Because APE1/Ref-1 is expressed and elevated in prostate cancer, we sought to characterize APE1/Ref-1 expression and activity in human prostate cancer cell lines and determine the effect of selective reduction-oxidation (redox) function inhibition on prostate cancer cells in vitro and in vivo . (oncotarget.com)
  • Due to the role of oncogenic transcriptional activators NFĸB and STAT3 in survivin protein expression, and APE1/Ref-1 redox activity regulating their transcriptional activity, we assessed selective inhibition of APE1/Ref-1's redox function as a novel method to halt prostate cancer cell growth and survival. (oncotarget.com)
  • Decreased expression of NBPF1 in neuroblastoma cell lines with loss of 1p36 heterozygosity and the marked decrease of anchorage-independent clonal growth of DLD1 colorectal carcinoma cells with induced NBPF1 expression further suggest that NBPF1 functions as tumor suppressor. (biomedcentral.com)
  • LC-MS/MS analysis was used to investigate the proteome of DLD1 colon cancer cells with induced NBPF1 expression. (biomedcentral.com)
  • Forced expression of NBPF1 in HEK293T cells resulted in a G1 cell cycle arrest that was accompanied by upregulation of the cyclin-dependent kinase inhibitor p21 CIP1/WAF1 in a p53-dependent manner. (biomedcentral.com)
  • Additionally, forced expression of NBPF1 in two p53-mutant neuroblastoma cell lines also resulted in a G1 cell cycle arrest and CDKN1A upregulation. (biomedcentral.com)
  • B ) Western blot analysis of the expression of NFkB-p65 and p53 in HeLa, SiHa, and ME-180 cells treated with MNBE at indicated dose for 24 h. (biomedcentral.com)
  • Induction of p21 expression by genotoxic stress and its role during terminal differentiation of various cell types have been investigated intensively. (bio2009.org)
  • Induction of apoptosis was associated with an increase in activation and expression of caspase 8 which then is believed to have induced cleavage of Bid into tBid. (upm.edu.my)
  • Western blot was used to detect the effect of G6PC overexpression on the cell -cycle protein expression in liver cancer cells . (bvsalud.org)
  • Whereas immunohistochemistry of the metastatic tumor showed a predominantly sarcomatous vimentin positive tumor that has lost E-cadherin expression, PF338 cells demonstrated biphasic growth and carried mutations in KRAS , PIK3CA , PTEN and ARID1A . (por-journal.com)
  • In UCS, several studies identified characteristic EMT-related expression signatures including active TGF-β signaling in tissue and cell lines [ 7 , 11 , 13 , 14 ]. (por-journal.com)
  • In this study, we report that surface expression of CD26, through its associated DPPIV enzyme activity, enhanced sensitivity of Jurkat T-cell transfectants to G 2 -M arrest induced by the chemotherapeutic drug, doxorubicin. (elsevierpure.com)
  • This was associated with disruption of cell cycle-related events, including hyperphosphorylation and inhibition of p34 cdc2 kinase activity, phosphorylation of cdc25C, and alteration in cyclin B1 expression. (elsevierpure.com)
  • p53 is a transcription factor whose expression is increased by DNA damage and blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)
  • Dmp1 is a unique tumor suppressor that activates Arf expression and thus induces p53-dependent cell cycle arrest. (eurekalert.org)
  • Expression of SHP-1 induced by hyperglycemia prevents insulin actions in podocytes. (ohri.ca)
  • Lonafarnib is also able to induce apoptosis via a CHOP-dependent upregulation of the DR-5-expression and a following caspase-8-activation. (uni-marburg.de)
  • Also our study indicated that WSC induced an increase in the transcriptional expression of matrix metalloproteinases, MMP-2 and MMP-9 and an immune response regulator, Toll Like Receptor-4. (who.int)
  • B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
  • This TCEE also demonstrates antitumor properties such as the induction of cell cycle arrest and activation of apoptosis on human colon, lung, melanoma, osteosarcoma, and pancreatic cancer cells [ 16 - 19 ]. (hindawi.com)
  • An integrated network pharmacology and RNA-Seq approach for exploring the preventive effect of Lonicerae japonicae flos on LPS-induced acute lung injury. (greenmedinfo.com)
  • Four varieties of tumor cells were examined: malignant melanoma A375, lung caner A549, ovarian carcinoma breast and A2780 cancer MCF-7 and MDA-MB-231 cells. (racetab.org)
  • The research, published by Cell Press in the October 2007 issue of Cancer Cell , may lead to development of new drug therapies for lung cancer. (eurekalert.org)
  • Non-small-cell lung cancers (NSCLCs) are the most common type of lung cancer, and scientists have identified distinct patterns of genetic alterations that are associated with this malignancy. (eurekalert.org)
  • Importantly, Dmp1 overexpression inhibited growth of human lung cancer cells with K-ras mutation and wild-type Arf and p53. (eurekalert.org)
  • In a cell-free system, Dox did not inhibit p34cdc2 kinase activity, indicating that is has no direct effect on the enzyme function. (aspetjournals.org)
  • In whole cells, Dox treatment prevented p34cdc2 kinase dephosphorylation without altering its synthesis, and this effect was due to neither down-regulation of cdc25C nor inhibition of protein-tyrosine phosphatase activity. (aspetjournals.org)
  • Representative colorectal cancer and non-cancerous cell lines were treated with dichloroacetate (DCA), an inhibitor of pyruvate dehydrogenase kinase. (dcaguide.org)
  • Pyruvate dehydrogenase kinase inhibition attenuates glycolysis and facilitates mitochondrial oxidative phosphorylation, leading to reduced growth of colorectal cancer cells but not of non-cancerous cells. (dcaguide.org)
  • In response to DNA damage, a synthetic lethal relationship exists between the cell cycle checkpoint kinase MK2 and the tumor suppressor p53. (nature.com)
  • Cell viability was determined using Cell Counting Kit-8 assay. (biomedcentral.com)
  • Viability assays and cell cycle analyses were used to test in vitro sensitivity to different standard and novel treatments. (por-journal.com)
  • Our data suggest that HDAC inhibition is effective in a novel UCS cell line by interfering with both viability and differentiation. (por-journal.com)
  • Outcomes Leucine deprivation decreases viability of tumor cells We 1st determined the result of leucine deprivation on viability of tumor cells. (racetab.org)
  • Open up in another window Shape 1 The result of leucine deprivation on cell viability(A and B) Cells had been incubated in charge BAY 293 (+leu) or leucine-deficient (?leu) moderate for indicated period, accompanied by MTT assay. (racetab.org)
  • By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
  • This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)
  • In vitro ubiquitination assay showed a decreased p27 ubquitination in PA-treated prostate cancer cells. (iiarjournals.org)
  • Transwell and scratch assay were used to observe the invasion and migration of liver cancer cells . (bvsalud.org)
  • Clone formation assay showed that the number of clones was significantly lower in AdG6PC than the other two groups (P liver cancer cells . (bvsalud.org)
  • Transwell assay showed that the number of cell migration was significantly lower in AdG6PC than the other two groups (P (bvsalud.org)
  • Open up in another window Shape 3 Leucine deprivation induces apoptosis of human being breast tumor BAY 293 cell(A and B) MDA-MB-231 or MCF-7 cells had been incubated in charge (+leu) or leucine-deficient (?leu) moderate for 48h, accompanied by dedication of cleaved caspases inside a and annexin-V assay in B. (C and D) Nude mice. (racetab.org)
  • The uptake of the purified MWCNTs was evaluated using fluorescence activated cell sorting (FACS), while the effects on cell fate were assessed using a 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt colorimetric assay, cell cycle and nanoindentation. (cdc.gov)
  • Frog egg cell extracts have been used extensively in cell cycle research because they are relatively large, reaching a diameter of 1mm, and so contain large amounts of protein, making protein levels more easily measurable. (wikipedia.org)
  • p27Kip1 protein levels are elevated in arresting cells. (wikipedia.org)
  • In human BCa tissues, we observed that LAPTM5 was significantly induced at both mRNA and protein levels, which is consistent with our microarray result. (spandidos-publications.com)
  • Our investigations are ongoing in biomarkers of resistance and potential new targets for therapeutic intervention in colorectal cancer, melanoma and Merkel cell carcinoma. (massgeneral.org)
  • It results in a single layer of arrested cells of arrested cells, and is a process that is notably missing in cancer cells. (wikipedia.org)
  • The ethanolic extract of T. camphoratus (TCEE) which contains abundant bioactive compounds including triterpenoids and polysaccharides also has antitumor effects in various human cancer cell lines. (hindawi.com)
  • Moreover, treatment with TCEE is found to enhance the cytotoxic effects of amphotericin B in human colon cancer cell both in vitro and in vivo [ 17 ]. (hindawi.com)
  • Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
  • Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
  • We have demonstrated that phenylacetate(PA)-induced cell cycle arrest in human prostate cancer is mediated by increase of p27. (iiarjournals.org)
  • Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
  • Scholars@Duke publication: Inhibition of Isoprenylcysteine Carboxylmethyltransferase Induces Cell-Cycle Arrest and Apoptosis through p21 and p21-Regulated BNIP3 Induction in Pancreatic Cancer. (duke.edu)
  • Cancer cells are highly dependent on glycolysis. (dcaguide.org)
  • Dichloroacetate reduced lactate levels in growth media and induced dephosphorylation of E1 α subunit of pyruvate dehydrogenase complex in all cell lines, but the intrinsic mitochondrial membrane potential was reduced in only cancer cells ( P =0.04). (dcaguide.org)
  • Early observations concerning the metabolic differences between cancer and normal cells were made by Otto Warburg, who showed that cancer cells are inherently dependent on glycolysis for production of chemical energy (Warburg, 1956). (dcaguide.org)
  • Andrographolide induces cell cycle arrest and apoptosis in PC-3 prostate cancer cells. (upm.edu.my)
  • Scratch repair rate was significantly lower in AdG6PC than the other two groups (P liver cancer cells . (bvsalud.org)
  • Baicalein Induces G2/M Cell Cycle Arrest Associated with ROS Generation and CHK2 Activation in Highly Invasive Human Ovarian Cancer Cells. (tku.edu.tw)
  • Plagioneurin B was isolated via conventional chromatography and tested for thorough mechanistic apoptosis activity on human ovarian cancer cells (CAOV-3). (um.edu.my)
  • Publishing in Cancer Cell 12, 381-394, October 2007. (eurekalert.org)
  • Cancer cells that are defective in p53 function are deficient in their ability to transcriptionally upregulate the CDK inhibitor p21 after genotoxic stress. (nature.com)
  • SKOV-3 and BG1 were used as ovarian cancer cell lines. (uni-marburg.de)
  • This study supports evidence in literature pointing to a certain activity of these drugs and at the same time emphasizes the different sensibility of human ovarian cancer cell lines to targeted therapeutics in vitro. (uni-marburg.de)
  • One such peptide, Lunasin , has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. (weeksmd.com)
  • Numerous vectors have been engineered for the sole purpose of killing cancer cells, and some have successfully suppressed malignant tumours. (weeksmd.com)
  • On the other hand, the infiltration of organs by a monoclonal population of aberrant cells, the possibility of lethal evolution, and the cancer-based modalities of successful treatment are all consistent with a neoplastic process. (medscape.com)
  • Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
  • Blockade of CHK2 activation by CHK2 inhibitors protects cells from baicalein-mediated G2/M cell cycle arrest. (tku.edu.tw)
  • Acetogenin group has attracted worldwide attention in the past few years due their biological abilities as inhibitors for several types of tumour cells. (um.edu.my)
  • Therefore, compared to normal p53-proficient cells, p53-defective cells are more reliant on MK2 activity, which drives an alternative cell cycle checkpoint pathway that stabilizes the CKI inhibitors p27 Kip1 and Gadd45α in order to maintain G 1 /S and G 2 /M arrest after certain types of DNA damage 16 , 18 . (nature.com)
  • A significant inhibition on cell growth, induction of apoptosis and cell cycle arrest were observed in 786-O and 769-P cells after kaempferol treatment compared with the control group. (nih.gov)
  • The induction of cell cycle arrest by a pheromone, chemically distinct from the one used to attract the opposite mating type, highlights the existence of a sophisticated mechanism to increase chances of mate finding, while keeping the metabolic losses associated with the release of an attraction pheromone to a minimum. (ugent.be)
  • β-peltatin initially arrested PAC cells at G2/M phase, followed by apoptosis induction. (biomedcentral.com)
  • Increase of Annexin V-FITC bound to phosphatidylserine confirmed the apoptosis induction in the cells. (um.edu.my)
  • Flavopiriol blocks the ATP-binding site of cyclin-dependent cinases and leads to a G1- respectively G2-cellcycle arrest in vitro. (uni-marburg.de)
  • In addition, NK cells stimulated with lunasin plus cytokines displayed higher tumoricidal activity than those stimulated with cytokines alone using in vitro and in vivo tumor models. (weeksmd.com)
  • Human fibroblast monocultures were found to be insufficient to fully replicate in vivo fibrosis outcomes suggesting in vitro predictive potential depends upon more advanced cell culture in vitro models. (cdc.gov)
  • ASncmtRNA knockdown with an antisense oligonucleotide induces massive apoptosis in tumor cell lines, without affecting healthy cells. (uandes.cl)
  • Kaempferol has been shown to inhibit cell growth, induce apoptosis and cell cycle arrest in several tumors, but not in renal cell carcinoma (RCC). (nih.gov)
  • Other reports also show that DN is highly associated with accelerated aging in various types of cells such as tubular cells, podocytes, mesangial cells, and endothelial cells [ 21 - 23 ]. (hindawi.com)
  • An alcohol-free fermented blueberry and blackberry beverage extract attenuates diet-induced obesity and blood glucose in mice. (greenmedinfo.com)
  • The efficiency of blackberry loaded AgNPs, AuNPs and Ag@AuNPs mediated pectin in the treatment of cisplatin-induced cardiotoxicity. (greenmedinfo.com)
  • In human somatic cells, the cell cycle lasts about 18 hours, and the G1 phase makes up about 1/3 of that time. (wikipedia.org)
  • The aims of this preclinical study are to evaluate the capability of TCEE to suppress human hepatocellular carcinoma cells and clarify the related antitumor effects. (hindawi.com)
  • CD26, a M r 110,000 surface-bound ectopeptidase with dipeptidyl peptidase IV (DPPIV) activity, has an array of diverse functional properties, with a role in T-cell physiology and the development of certain human cancers. (elsevierpure.com)
  • In addition, we demonstrate that the addition of exogenous soluble DPPIV enhanced sensitivity of lymphoid tumor cell lines to doxorubicin, suggesting a potentially useful clinical role for CD26/DPPIV in the treatment of selected human hematological malignancies. (elsevierpure.com)
  • Previous studies have found that DIM can improve type 2 diabetes by enhancing glucose uptake through the activation of insulin signaling in 3T3-L1 cells, and by lowering the plasma glucose levels in high-fat-diet-fed obese mice [13, 14]. (researchgate.net)
  • Type 1 diabetes mellitus is a metabolic disease resulting from the destruction of insulinproducing β cells in the pancreas, that leads to hyperglycemia [1,2,20]. (researchgate.net)
  • Our results showed that exposure to MWCNTs reduced cell metabolic activity and induced cell cycle arrest. (cdc.gov)
  • In cell culture, serum is the growth medium in which the cells are grown and contains vital nutrients. (wikipedia.org)
  • In the present study, we investigated the effects of kaempferol and the underlying mechanism(s) on the cell growth of RCC cells. (nih.gov)
  • In the present study, the TCEE treatment induced cell cycle arrest and suppressed cell growth on both Hep3B and HepJ5 cells. (hindawi.com)
  • Moreover, cell cycle arrest at G0/G1 phase was triggered by decreased LAPTM5 as well, which could lead to delayed BCa cell growth. (spandidos-publications.com)
  • Animal study confirmed that β-peltatin significantly suppressed the growth of subcutaneously-implanted BxPC-3 cell xenografts. (biomedcentral.com)
  • LIN28, which can be an RNA-binding protein consisting of LIN28A and LIN28B,17 is definitely an important regulator of miRNAs and mRNAs.18,19 LIN28 regulates not simply the translation of mRNAs that play a important function in cell growth and metabolism but also the biogenesis of miRNAs. (cathepsin-s.com)
  • The growth rate of the cells was reduced when grown in ( significantly? (racetab.org)
  • and regulating cell growth (1) . (oregonstate.edu)
  • Its proposed mechanism of action is an iron/zinc chelator that depletes iron within the cell. (wikipedia.org)
  • The in vivo effects of β-peltatin and podophyllotoxin were evaluated on a subcutaneously-xenografted BxPC-3 cell nude mice model. (biomedcentral.com)
  • Objective Indole‐3‐carbinol (I3C), a naturally occurring compound found in cruciferous vegetables, and its metabolite 3,3′‐diindolylmethane (DIM) reduce body mass and serum glucose levels in high‐fat‐diet‐induced obese mice. (researchgate.net)
  • In this study, we investigated whether DIM could improve insulin-dependent diabetes and nephropathy in streptozotocin (STZ)-induced diabetic mice. (researchgate.net)
  • Moreover, I3C exerts anti-obesity effects by reducing body weight and fat accumulation in epididymal adipose tissue in HFD-induced obese mice and thereby improves hyperglycemia and hyperinsulinemia [126]. (researchgate.net)
  • Cp/Heph mutant mice have iron-induced neurodegeneration diminished by deferiprone. (upenn.edu)