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  • limbs
  • We screened the blastema of resected axolotl limbs with a cross-reactive mouse cytokine protein detection array, revealing a rapid induction of cytokines, chemokines, and inflammatory markers within 1 d of limb resection. (pnas.org)
  • Nerve axons and the apical Bcl-w epidermal cap (AEC) are both essential for the formation of an accumulation blastema by amputated limbs of urodele salamanders. (assayway.com)
  • We have found that pseudotyped Maloney Murine Leukemia Virus (MMLV) retroviruses can very efficiently infect axolotl cells in vitro and in vivo , and can be used for both lineage and functional analyses in regenerating limbs. (biologists.org)
  • tissue regeneration
  • Plant cells have a remarkable plasticity that allows cellular reprogramming from differentiated cells and subsequent tissue regeneration. (bioportfolio.com)
  • Using the ear hole closure phenotype, a genetically mapped and reliable quantitative indicator of regeneration in the MRL mouse, we show that the unrelated Cdkn1a tmi/Tyj /J p21 −/− mouse (unlike the B6129SF2/J WT control) closes ear holes similar to MRL mice, providing a firm link between cell cycle checkpoint control and tissue regeneration. (pnas.org)
  • renal
  • Because wilms- tumor, a pediatric renal cancer, initiates from retention of renal stem cells, we hypothesized that surface antigens previously up-regulated in microarrays of both HFK and blastema-enriched stem-like wilms- tumor xenografts NCAM, ACVRIIB, DLK1-PREF, GPR39, FZD7, FZD2, NTRK2 are likely to be relevant markers. (duhnnae.com)
  • These results and concepts provide a framework for developing cell selection strategies for human renal cell-based therapies. (duhnnae.com)
  • receptor
  • Methods -WCCS-1 cells were cultured in vitro and exposed to IGF-I and IGF-II, as well as their antagonists, IGF binding protein 2 and the type I receptor blocking antibody IGF-IRα. (bmj.com)
  • Finally, the addition of IGFBP-2 or the type 1 receptor blocking antibody IGF-IRα partly abrogated the death inducing effects of IGF-I and IGF-II. (bmj.com)
  • Cell surface, heparin-like molecules are required for binding of basic fibroblast growth factor to its high affinity receptor. (springer.com)
  • These viruses, when modified by changing their coat protein, can infect axolotl cells only when they have been experimentally manipulated to express the receptor for that coat protein, thus allowing for the possibility of targeting specific cell types. (biologists.org)
  • Viral gene transfer is an attractive alternative, as it can provide stable gene expression and can, in principle, be targeted to specific cell types based on the availability of a receptor. (biologists.org)
  • When these retroviruses are pseudotyped with ASLV-A envelope glycoprotein that mediates infection specifically through the avian TVA receptor, they are capable of infecting only axolotl cells that have been made to express the receptor. (biologists.org)
  • cytokines
  • Macrophages are an important source of both inflammatory and anti-inflammatory signals, arriving in mammalian wounds 48-96 h after injury, where they clear dead cells, release proinflammatory cytokines, and subsequently produce factors that dampen inflammation and stimulate angiogenesis, fibroblast migration, and replication ( 17 ). (pnas.org)
  • The JNK signal transduction cascade is activated by exposure of cells to cytokines or environmental stress. (biomedcentral.com)
  • morphogenesis
  • Our new special issue is packed with articles that use mathematical and physical approaches to gain insights into cell and tissue patterning, morphogenesis and dynamics, and that provide a physical framework to capture these processes operating across scales. (biologists.org)
  • nerve-independent for morphogenesis, but remain dependent on the nerve for blastema growth. (assayway.com)
  • undergo
  • Mitotic cells might undergo senescence by failing to replicate. (hindawi.com)
  • S-phase indices were comparable, irrespective of whether the cells were exposed to IGFs or not, which suggests that WCCS-1 cells undergo cell death at random during the cell cycle rather that from the prereplicative phase. (bmj.com)
  • myogenic
  • Transgenic animals also had elevated levels of cells coexpressing markers of SC lineage and myogenic commitment at sites of muscle damage. (pnas.org)
  • processes
  • At the molecular level, in fact, a gradual weakening of the cellular processes regulating cardiovascular homeostasis occurs in aging cells. (hindawi.com)
  • therefore, these cells are of great interest for the study of these processes. (jcancer.org)
  • The gene functions at the interface between survival and cell death during stress-inducing processes like regeneration and starvation in sexual and asexual races of planarians. (pnas.org)
  • By analogy to other organisms, apoptosis has been thought to be the mechanism by which cell number is reduced during the remodeling processes associated with the continuous adaptation of the animal to new body sizes during starvation. (pnas.org)
  • progeny
  • Together with their immediate progeny, neoblasts account for ≈15-25% of all parenchymal cells ( 4 ). (pnas.org)
  • mammals
  • Subsequently, emx1 is expressed in the telencephalon, as reported in mammals, and can be detected in the olfactory placode and in a small group of cells in the forebrain at 25 hpf. (sdbonline.org)
  • HeLa
  • DAP-1, together with DAP-kinase, has been identified as a positive mediator of programmed cell death induced by γ-IFN in HeLa cells. (pnas.org)
  • To address the mechanisms by which viruses induce and interact with the autophagy pathway, I am using poliovirus infection in HeLa cells that have several key autophagy genes knocked out by Crispr-Cas9. (jccfund.org)
  • phenotype
  • Aim -To study the effects of insulin-like growth factors (IGFs) on the growth phenotype of a Wilms's tumour cell line (WCCS-1). (bmj.com)
  • structures
  • As we know interaction between cells and ECM provides suitable three-dimensional structures. (zjrms.com)
  • Recent studies provide evidence that the pattern-forming cells synthesize an information-rich extracellular matrix (ECM) that controls the behavior of pattern-following cells leading to the regeneration of limb structures. (springer.com)
  • bone
  • In contrast, we find that Lgr6 is expressed within cells of the nail matrix portion of the nail epithelium, as well as in a subset of cells in the bone and eccrine sweat glands. (nih.gov)
  • These data implicate mIGF-1 as a powerful enhancer of the regeneration response, mediating the recruitment of bone marrow cells to sites of tissue damage and augmenting local repair mechanisms. (pnas.org)
  • Although in lethally irradiated mice, bone marrow-derived SCs replenish the depleted satellite cell pool and subsequently incorporate effectively into exercised skeletal muscle ( 9 ), less is known about the ability of bone-marrow-derived SCs to ameliorate muscle damage under more clinically relevant conditions. (pnas.org)
  • Indeed, the transplantation of bone marrow-derived SCs into the mdx dystrophic mouse model had a limited impact on muscle cell replacement ( 10 ), suggesting that the poor recruitment of circulating SCs is one of the limiting factors for tissue repair ( 10 , 11 ). (pnas.org)
  • The SP from bone marrow cells was isolated from femur and tibia of 8-week-old c -kit / GFP transgenic mice by fluorescence-activated cell sorter (FACS) analysis. (pnas.org)
  • lineage
  • Genetic lineage analysis reveals that Lgr6-expressing cells give rise to the nail during homeostatic growth, demonstrating that Lgr6 is a marker of nail stem cells. (nih.gov)
  • I am interested in developing methods for recording lineage and transcriptional event histories within the genome of the cells. (jccfund.org)
  • However, very few molecular tools exist for studying crucial cell lineage relationships over regeneration or for robust and sustained misexpression of genetic elements to test their function. (biologists.org)
  • pathways
  • But despite the importance of tissue remodeling, we do not yet understand the mechanisms that coordinate individual cell regulatory pathways into tissue- and organ-level organization. (biologists.org)
  • Because of the role of p53 in certain apoptotic pathways, these cells may not go into apoptosis in the absence of p53. (aacrjournals.org)