• First, we found that GS-002 significantly inhibited cell proliferation and induced cell apoptosis in dose-dependent manners. (hindawi.com)
  • ATF-3 induces DNA synthesis and expression of cyclin D1 in hepatocytes [ 21 ] and is involved in serum-induced cell proliferation as a target gene of c-myc [ 10 ]. (hindawi.com)
  • ACNVs were found to possess specific antitumor cell proliferation activity associated with an apoptosis-inducing pathway. (dovepress.com)
  • Exhibiting high consistence in sequence and structure, S100 family members are interchangeable in function and they show a wide spectrum of biological processes, including proliferation, apoptosis, migration, inflammation and differentiation and the like. (cancerindex.org)
  • To investigate the effects of silibinin on the growth of oral cancer cells, cell proliferation and anchorage-independent colony formation tests were conducted on YD10B and Ca9-22 oral cancer cells. (jcancer.org)
  • Silibinin effectively suppressed YD10B and Ca9-22 cell proliferation and colony formation in a dose-dependent manner. (jcancer.org)
  • Sorafenib (Sor) can block receptor tyrosine kinases, which are involved in the development of new blood vessels, as well as cell proliferation as a result [ 22 ]. (biomedcentral.com)
  • Inhibitory effect of EESR on cancer cell growth and proliferation was determined by water-soluble tetrazolium salt assay. (jcpjournal.org)
  • EESR possessing antioxidant activity efficiently inhibits proliferation of HT29 cells by inducing both cell cycle arrest and apoptosis. (jcpjournal.org)
  • 6 , 7 Therefore, the inhibition of cancer cell proliferation by inducing cell cycle arrest and apoptosis is being an important preventive strategy against cancer. (jcpjournal.org)
  • Several main apoptotic indicators were found in GS-002-treated cells, such as the cleaved forms of caspase-3, caspase-9, and poly(ADP-ribose) polymerase (PARP). (hindawi.com)
  • As part of toxicity studies, localization of SNP in Hep G2 cell line, cell viability, biochemical effects and apoptotic/necrotic potential were assessed. (nih.gov)
  • NC exhibited rapid cellular uptake and stronger apoptotic effect (~46.6% reduced IC 50 value) than FC, blocking HepG2 cells at G2/M phase. (bgu.ac.il)
  • EESR-induced apoptosis was associated with the upregulation of p53, a death receptor Fas, and a pro-apoptotic protein Bax and the activation of caspase 3, 8, and 9, resulting in the degradation of PARP. (jcpjournal.org)
  • In vivo antitumor activity and toxicity were evaluated in Hep G2 cell xenograft model. (dovepress.com)
  • In contrast, WI-38 cells were far more sensitive to the toxicity of Sor. (biomedcentral.com)
  • Our in vivo studies demonstrated NC to be more bio-available than FC and showed a better tissue distribution profile without inducing any toxicity (100mg/kg bw) in mice. (bgu.ac.il)
  • 1998. Protection by extracellular glutathione against sulfur mustard induced toxicity in vitro. (cdc.gov)
  • 1998. Protection of human upper respiratory tract cell lines against sulphur mustard toxicity by hexamethylenetetramine (HMT). (cdc.gov)
  • Cytotoxicity, antiproliferative and apoptosis-inducing capacity of ACNVs against hepatoma carcinoma cell were assessed. (dovepress.com)
  • To investigate the mechanism of EESR-mediated cytotoxicity, HT29 cells were treated with various concentrations of EESR and the induction of cell cycle arrest and apoptosis was analyzed by flow cytometry, 4,6-diamidino-2-phenylindole staining, and Western blot analysis. (jcpjournal.org)
  • C7a, a biphosphinic cyclopalladated compound, efficiently controls the development of a patient-derived xenograft model of adult T cell leukemia/lymphoma. (fiocruz.br)
  • These include regulation of cell cycle, activation of mitochondrial pathway, induction of mitochondrial outer membrane permeabilization, induction of apoptosis, modulation of oxidative stress, amelioration of inflammation, modulation of insulin signaling and inhibition of angiogenesis. (mdpi.com)
  • 4-Methoxyphenyl)(3,4,5-trimethoxyphenyl)methanone inhibits tubulin polymerization, induces G2/M arrest, and triggers apoptosis in human leukemia HL-60 cells. (fiocruz.br)
  • GS-002 also induced endoplasmic reticular (ER) stress as evidenced by increases in ER stress-responsive proteins including glucose-regulated protein 78 (GRP78), growth arrest- and DNA damage-inducible gene 153 (GADD153), phosphorylated eukaryotic initiation factor 2 α (eIF2 α ), phosphorylated protein endoplasmic-reticular-resident kinase (PERK), and ATF-3. (hindawi.com)
  • Moreover, it induced cell cycle arrest in the G0/G1 phase, apoptosis, and ROS generation in these cells. (jcancer.org)
  • Silibinin considerably reduced the development of oral cancer cells by inducing apoptosis, G 0 /G 1 arrest, ROS generation, and activation of the JNK/c-Jun pathway. (jcancer.org)
  • A substantial synergistic interaction between Amy and Sor was observed (CI 50 = 0.56), which was connected to cell cycle arrest at the S and G2/M stages and increased apoptosis and potential necroptosis. (biomedcentral.com)
  • EESR induced cell cycle arrest at G2/M phase in a dose-dependent manner by modulating cyclin B, cyclin-dependent kinase 1 (CDK1), and CDK inhibitor p21 expression. (jcpjournal.org)
  • Whereas damage signals of cells activate p53 via CHK2 to repair damaged DNA, leading to cell cycle arrest. (jcpjournal.org)
  • In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro . (hindawi.com)
  • Therefore, the current study used integrated in vitro and in silico approaches to figure out Amy and Sor's possible synergistic activity in targeting AMPK/mTOR and BCL-2 for anti-angiogenesis and apoptosis cell death in HepG2 cells. (biomedcentral.com)
  • We applied an in vitro model of HepG2 cells induced by glucolipotoxicity and evaluated the effects of MLE and its major components nCGA, Crp, and CGA. (bvsalud.org)
  • In breast cancer, ATF-3 enhances cancer cell-initiating features [ 22 ] and is associated with activation of the canonical Wnt/ β -catenin pathway [ 23 ]. (hindawi.com)
  • Western blotting revealed that silibinin downregulated SOD1 and SOD2 and triggered the JNK/c-Jun pathway in oral cancer cells. (jcancer.org)
  • Whilst U87MG shared the same dampened p53 activity, upon chemotherapeutic drug treatment in chronic hypoxic conditions, these cells used a different mechanism, independent of the DNA damage pathway. (biomedcentral.com)
  • Although the dosages of Ab cannot decrease the blood sugar in db/db mice, our results indicate that Ab alleviates glucolipotoxicity-induced DN by inhibiting kidney fibrosis-related proteins through the Ras/ERK pathway. (bvsalud.org)
  • 8 , 9 Cell cycle program is regulated by cyclin/cyclin-dependent kinase (CDK) complexes. (jcpjournal.org)
  • 10 , 11 The major molecules responsible for G2/M transition are cyclin A /CDK1 and cyclin B/CDK1. (jcpjournal.org)
  • 12 The activation of cyclin/CDK complex accelerates cell cycle progression, which are regulated by phosphorylation. (jcpjournal.org)
  • 13 The expression of CDK inhibitor p21 is upregulated by activated p53, resulting in the suppression of G2/M transition by the inactivation of cyclin/CDK complex. (jcpjournal.org)
  • The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)
  • These results suggest that the induction of apoptosis by the propolis derivative, GS-002, is partially mediated through ER stress and ATF-3-dependent pathways, and GS-002 has the potential for development as an antitumor drug. (hindawi.com)
  • In the past decade, induction of apoptosis has become the major strategy to combat cancer. (hindawi.com)
  • Therefore, a search for innovative strategies other than induction of apoptosis is urgently needed. (hindawi.com)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • We applied a range of molecular and cellular techniques to measure cell survival, cell cycle progression, protein expression and DNA damage combined with a transcriptomic micro-array approach in D283-MED cells, for global gene expression analysis in acute and chronic hypoxic conditions. (biomedcentral.com)
  • EESR showed significant antioxidant activity and inhibitory effect on HT29 cell growth in a dose-dependent manner. (jcpjournal.org)
  • Further, SNP induce apoptosis at concentrations up to 250 microg/mL, which could favor scarless wound healing. (nih.gov)
  • The endocytosis mechanism of ACNVs was evaluated on Hep G2 cells in the presence of different endocytosis inhibitors. (dovepress.com)
  • An integrated network pharmacology and RNA-Seq approach for exploring the preventive effect of Lonicerae japonicae flos on LPS-induced acute lung injury. (greenmedinfo.com)
  • In D283-MED and U87MG, chronic hypoxia (5 days), but not acute hypoxia (24 h) induced resistance to chemotherapy and X-ray irradiation. (biomedcentral.com)
  • 1993. Sulfur mustard inhalation induced respiratory lesions in guinea pigs: Physiological, biochemical and histological study. (cdc.gov)
  • An herbal formula inhibits STAT3 signalling and attenuates bone erosion in collagen-induced arthritis. (greenmedinfo.com)
  • The effects of silibinin on the migration and invasion of oral cancer cells were evaluated using transwell assays. (jcancer.org)
  • Furthermore, silibinin inhibited the migration and invasion abilities of YD10B and Ca9-22 cells by regulating the expression of proteins involved in the epithelial-mesenchymal transition. (jcancer.org)
  • ACNVs could be internalized into tumor cells mainly via phagocytosis, but they were quickly cleared once entering the blood. (dovepress.com)
  • The microenvironment of the cell and the interactions of many growth factors have a role in tumor development and survival [ 2 , 7 ]. (biomedcentral.com)
  • Together our results demonstrate a new mechanism explaining hypoxia-induced resistance involving the alteration of the response to DSB in D283-MED cells, but also highlight the cell type to cell type diversity and the necessity to take into account the differing tumour genetic make-up when considering re-sensitisation therapeutic protocols. (biomedcentral.com)
  • The objective of this study is to evaluate the antioxidant and anticancer effects of ethanol extract of S. rufopilosa (EESR) and to determine the molecular mechanism of its anticancer activity in human colon carcinoma HT29 cells. (jcpjournal.org)
  • Extracellular vesicles (EVs) are a group of cell-derived membranous structures, which play an important role in intercellular communication and functional transduction. (dovepress.com)
  • 1 , 2 In recent years, mammalian cell-derived exosomes (a type of extracellular vesicle) have been widely used in the field of biomedicine, including diagnosis, regulation of disease progression and drug delivery. (dovepress.com)
  • These effects of honey have been thoroughly investigated in certain cancers such as breast, liver and colorectal cancer cell lines. (mdpi.com)
  • Reactive oxygen species play a vital role in hyperglycemia-induced renal injury. (bvsalud.org)
  • However, resistance to apoptosis is considered to be a characteristic of several types of cancers. (hindawi.com)
  • This acquired resistance upon chronic hypoxia was present but less pronounced in MEB-Med8A cells. (biomedcentral.com)
  • Poor oral bioavailability of chelidonine, a bio-active ingredient of Chelidonium majus, showing anti-cancer potentials against cancer cells with multidrug resistance, makes its optimal use rather limited. (bgu.ac.il)
  • The major risk factors include chronic hepatitis B virus (HBV) infection, chronic hepatitis C virus (HCV) infection, environmental carcinogens such as aflatoxin B1 (AFB1), alcoholic cirrhosis, and inherited genetic disorder such as hemochromatosis, Wilson's disease, and tyrosinemia. (hindawi.com)
  • 3 , 4 Similarly, plant cells also secrete exosome-like nanovesicles (ELNVs) that can participate in various physiological processes such as the inheritance of traits and defense response. (dovepress.com)
  • Recent research demonstrated that the potential to induce apoptosis through endoplasmic reticular (ER) stresses can be a target for cancer therapy. (hindawi.com)
  • Although they do not directly affect blood glucose level, MLE and nCGA show significant potential in managing glucolipotoxicity-induced diabetic nephropathy by targeting lipid metabolism and key molecular pathways. (bvsalud.org)
  • Previous studies reported that ATF-3 was induced by treating cells with antitumorigenic compounds [ 14 - 18 ] and a phosphoinositide 3-kinase inhibitor [ 19 ]. (hindawi.com)
  • Using transcriptomic analysis in D283-MED cells, we found a large transcriptional remodelling upon long term hypoxia, in particular the expression of a number of genes involved in detection and repair of double strand breaks (DSB) was altered. (biomedcentral.com)
  • High-fat diet-fed db/db mice were employed as a model for glucolipotoxicity-induced diabetic nephropathy. (bvsalud.org)
  • Exosomes are a type of membrane vesicles secreted by living cells. (dovepress.com)
  • To address this problem, we encapsulated chelidonine in biodegradable poly(lactide-co-glycolide) (PLGA) polymers and evaluated nano-chelidonine's (NCs) anti-cancer efficacy vis-à-vis free chelidonine (FC) against HepG2 cells and also evaluated its bioavailability in mice. (bgu.ac.il)
  • Activating transcription factor-(ATF-) 3, a stress-inducible transcription factor, is rapidly upregulated under various stress conditions and plays an important role in inducing cancer cell apoptosis. (hindawi.com)
  • The abnormal cell growth is one of the critical characteristics of cancer for invasion or spreading out to other parts of the body. (jcpjournal.org)
  • Cancer cells can grow unusually via limitless number of cell division and can avoid apoptosis. (jcpjournal.org)
  • On the other hand, ATF-3 is rapidly induced in cells treated with growth stimulators such as serum and growth factors [ 20 ]. (hindawi.com)