• Background: The authors developed an indicator dilution technique for small animals to repeatedly determine cardiac output and blood volume without cardiac instrumentation or blood sampling. (johnshopkins.edu)
  • Conclusions: Fluorescent dye dilution and video microscopy can be used to repeatedly determine cardiac output or blood volume in small animals. (johnshopkins.edu)
  • Rivers, RJ , Beckman, JB & Frame, MDS 2001, ' Technique for using video microscopy and indicator dilution for repeated measurements of cardiac output in small animals ', Anesthesiology , vol. 94, no. 3, pp. 489-495. (johnshopkins.edu)
  • Quantification of glyphosate and other organophosphorus compounds in human urine via ion chromatography isotope dilution tandem mass spectrometry. (cdc.gov)
  • Same as in NHANES 2011-2014, population folate status in the 2015-2016 survey cycle was assessed by a combination of two analytical methods: whole-blood folate was measured by microbiologic assay, while serum folate forms were measured by isotope-dilution high performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). RBC folate was then calculated using the data from both assays. (cdc.gov)
  • Five folate forms, 5-methyl-tetrahydrofolate, folic acid, 5-formyl-tetrahydrofolate, tetrahydrofolate, and 5,10-methenyl-tetrahydrofolate are measured by isotope-dilution high performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) (Fazili, et al. (cdc.gov)
  • It can be calculated by multiplying the stroke volume (SV, the amount of blood pumped by the left ventricle in one contraction) by the heart rate (HR, beats per minute), though other methods can be used to calculate CO (Fick technique, Indicator-dilution technique, Pulmonary artery catheterization with thermodilution). (cms.gov)
  • by measuring the proton spin relaxation rate as a function of magnetic induction field in a water sample where dipole-dipole couplings are suppressed by H/D isotope dilution. (lu.se)
  • These include the pulse pressure analysis, the transpulmonary thermodilution, the indicator dilution, the esophageal Doppler, the thoracic electrical bioimpedance, the carbon dioxide rebreathing, and the echocardiography. (biomedcentral.com)
  • Proper aseptic procedures should be observed, otherwise contaminants may be accidentally introduced during the injection of the broth culture and during serial dilution and plating. (bio-fit.eu)
  • The antibacterial activity of M. alternifolia EO was evaluated by the broth dilution method, by which minimum inhibitory and bactericidal concentrations (MIC and MBC) were determined. (bvsalud.org)
  • As an alternative to solely monitoring Ca 2+ changes using fluorescent indicators, scientists may want to rapidly raise or lower the intracellular Ca 2+ concentration and study the physiological response that results. (thermofisher.com)
  • Three of Bonde's attributes (1, 2, and 4) address the relationship between indictor organisms and pathogens of concern, while the remaining five describe desirable properties associated with quantifying the indicator. (nationalacademies.org)
  • Grow widely independent of other organisms present, when inculcated in artificial media (i.e., indicator bacteria should not be seriously inhibited in their growth by the presence of other bacteria). (nationalacademies.org)
  • 4.3.2 Problems Associated with Using Fecal Coliforms as Indicators of Pathogenic Organisms. (taylorfrancis.com)
  • Not all organisms grow sufficiently rapidly for agar gel diffusion techniques. (vin.com)
  • Thus, tube dilution data seldom often is not provided for anaerobic organisms. (vin.com)
  • Generally, only rapidly growing aerobic organisms are conducive to testing using these methods, and particularly tube dilution methods. (vin.com)
  • Pure, vibrant (meaning special media was not needed to coax the growth of the organism) of a large number of organisms are indicators of infection. (vin.com)
  • Personnel should be trained specifically in culture techniques and hospitals that provide this service (as do diagnostic labs) should maintain well designed and adequately collected quality control data to validate their procedures (NCCLS provides control organisms). (vin.com)
  • Historic definitions of microbial indicators, such as coliforms, have been tied to the methods used to measure them. (nationalacademies.org)
  • Two indicators, FITC-labeled hydroxyethyl starch (FITC-HES) and radioiodinated rhesus serum albumin (125I-RhSA), were injected simultaneously, followed by serial blood collection. (docksci.com)
  • Students will learn about concentrated solutions, practice making different serial dilutions and use simple math skills to calculate the solution concentration at each step. (novartis.com)
  • Serial dilutions range from 70243.90 µg/mL to 26.14 µg/mL. (bvsalud.org)
  • The most important biological attribute is a strong quantitative relationship between indicator concentration and the degree of public health risk. (nationalacademies.org)
  • 1987). An alternative means of demonstrating the relationship to health risk is through correlation between prospective indicator concentration and pathogen levels (Gerba et al. (nationalacademies.org)
  • The MIC evaluation was performed in 96-well microplates, in which 100 µL of Brain Heart Infusion (BHI), 100 µL of the EO dilution and 5 µL of the inoculum (final concentration = 5x105 CFU/mL) were inserted. (bvsalud.org)
  • Thus, separate criteria allow one to choose the indicator with the most desirable biological attributes for a given application and then match this with a measurement method that best meets the need of the application. (nationalacademies.org)
  • Box 4-2 lists desirable biological attributes of indicators and Box 4-3 lists desirable attributes of methods. (nationalacademies.org)
  • The process of validating an isolator decontamination process is discussed in a white paper by Mesa Labs ( Vaporized Hydrogen Peroxide Isolator Decontamination in a World of Uncertainty ), and therein, the selection and qualification of biological indicators (BIs) was covered in moderate detail. (mesalabs.com)
  • More importantly, a variety of new methods are becoming increasingly available, providing several options for measuring each indicator group. (nationalacademies.org)
  • We evaluated the less invasive arterial double indicator dilution method (TDD) as an alternative. (biomedcentral.com)
  • The recommended counting technique in this case uses the drop-plate method. (bio-fit.eu)
  • What is the use-dilution method? (answerbag.com)
  • Although Aromatherapy is a natural product, specific techniques for each type of population need to respect the method of intervention, dilution, time of use, and the frequency with which it is used. (bvsalud.org)
  • In this molecular expedition of the bitter taste receptor gene, students will use laboratory techniques and equipment to extract DNA from human cells, amplify a portion of the bitter taste receptor gene by PCR, and use gel electrophoresis to analyze DNA fragments. (novartis.com)
  • CBM was identified by the absence of subculture growths (50 µL) of dilutions equal to or greater than MIC. (bvsalud.org)
  • Specific inhalation techniques are necessary for proper use of the available types of inhaler device and less than optimal technique can result in decreased drug delivery. (ijpsr.com)
  • Besides being localized in alveolar macrophages, iNOS is also found in alveolar epithelial cells, silicotic granulomas, and histiocytic aggregates in BAL T. These results correspond to other indicators of lung damage seen after silica inhalation in rats. (cdc.gov)
  • we used immunohistochemical techniques to determine whether iNOS is upregulated in alveolar macrophages and other lung sources in response to silica. (cdc.gov)
  • The grape samples were hydrolyzed under acidic conditions, and the released odorants were studied by aroma extract dilution analysis (AEDA). (oregonstate.edu)
  • Dilution of ingested or cutaneously synthesized vitamin D in the large fat mass of obese patients fully explains their typically low vitamin D status. (nih.gov)
  • CD4+ T-lymphocyte levels are also used as prognostic indicators in patients with human immunodeficiency virus (HIV) disease (8). (cdc.gov)
  • Microbial water quality indicators are used in a variety of ways within public health risk assessment frameworks, including assessment of potential hazard, exposure assessment, contaminant source identification, and evaluating effectiveness of risk reduction actions. (nationalacademies.org)
  • This chapter describes desirable attributes of an indicator, typical applications of indicators, indicator attributes that are appropriate for such applications, and provides an assessment of whether current indicators and indicator approaches are meeting the needs of each application. (nationalacademies.org)
  • Chiu WK, Vien BS, Russ M, Fitzgerald M. Towards a Non-Invasive Technique for Healing Assessment of Internally Fixated Femur. (umassmed.edu)
  • We used indicator dilution methodology to determine the BV of 20 adult rhesus macaques (10 male, 10 female) that varied widely in body condition. (docksci.com)
  • They will use indicator solutions to determine the pH of common household products, and discover how pH is a property that scientists use in their experiments. (novartis.com)
  • Stoichiometry and the relation to classical analytical techniques such as titrimetry and gravimetry. (kent.ac.uk)
  • By introducing drug into the bloodstream very quickly, it produces a transient peak in arterial drug levels as the drug passes for the first time through the body, before its dilution into the full circulatory volume and distribution into tissues. (ijpsr.com)
  • Preload with this technique is determinated by measuring intrathoracic blood volume index (ITBVI) and global enddiastolic volume index (GEDVI) instead of filling pressures CVP and PCWP. (biomedcentral.com)
  • The odorants released with high flavor dilution values were 1-hexanal, β-damascenone, guaiacol, and vanillin, together with C6-aldehydes and -alcohols, 4-vinylguaiacol, 4-vinylphenol, and 1-octen-3-one. (oregonstate.edu)
  • Each patient provided a saliva sample (control group S) and two samples of dental biofilm from amalgam or resin-based composite restorations in the same patient, one obtained with the toothpick technique (group TT), and the other from homologous tooth restoration with the tray technique (group TR). (bvsalud.org)
  • The current ratio is a key indicator of a company's short-term financial health, as it gauges the ability to cover short-term liabilities with short-term assets. (yahoo.com)
  • This contaminants are also detectable on the utilized indicator media and their number should be reported together with the number of viable cells as additional measure of the quality. (bio-fit.eu)
  • Based on Frank-Starling law, ITBVI, GEDVI, CVP, and PCWP have been investigated on their usefulness as preload indicators and for this purpose correlated with stroke volume index (SVI). (biomedcentral.com)
  • It is beyond the scope of this manuscript to delineate proper techniques of specimen culture collection, but close adherence to recommended procedures including but not limited to site selection, site preparation and sample handling are critical to proper interpretation. (vin.com)
  • Culture Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uams.edu)
  • This graph shows the total number of publications written about "Culture Techniques" by people in UAMS Profiles by year, and whether "Culture Techniques" was a major or minor topic of these publications. (uams.edu)
  • Below are the most recent publications written about "Culture Techniques" by people in Profiles over the past ten years. (uams.edu)
  • For almost 40 years, Bonde's (1966) attributes of an ideal indicator have served as an effective model of how a fecal contamination index for public health risk and treatment efficiency should function ( Box 4-1 ). (nationalacademies.org)
  • BF in the lower abdomen (BF Ab ) is potentially a useful indicator of the influence of comprehensive BF redistribution in cardiovascular and hepato-gastrointestinal disease, in the postprandial period, and in relation to physical exercise. (biomedcentral.com)
  • Personnel should be required to demonstrate competency with device reprocessing (i.e., trainer observes correct technique) prior to being allowed to perform reprocessing independently. (cdc.gov)
  • When new devices or protocols are introduced, including changes in the manufacturer's instructions for use during the device's life cycle o Personnel should be required to demonstrate competency with device reprocessing (i.e., trainer observes correct technique) prior to being al owed to perform reprocessing independently. (cdc.gov)