Incubated with the primary antibody. Medical search. Frequent questions

Graves' disease (GD), characterized by TSH-receptor stimulating antibodies and increased thyroid hormone serum levels, is an autoimmune disease affecting roughly 3% of the general population ( 1 ). (frontiersin.org)
Kits include Biotinylated Secondary Antibodies , Serum Blocking Reagent, Peroxidase Blocking Reagent, Avidin Blocking Reagent, Biotin Blocking Reagent, High Sensitivity Streptavidin-HRP Conjugate (HSS-HRP), and Chromogen Solution. (rndsystems.com)
No antibody was detected against non-immunoglobulin serum proteins. (jacksonimmuno.com)
Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. (vectorlabs.com)
RESULTS: Rabies virus antibodies present in serum and cerebrospinal fluid were non-neutralizing. (cdc.gov)
In autoimmune adrenal disease, serum antibodies against several steroidogenic enzymes-including P450scc (CYP11A1, side-chain cleavage enzyme), P450c17 (CYP17, 17-alpha-hydroxylase), and P450c21 (CYP21A2, 21-hydroxylase)-are present. (medscape.com)
[ 3 , 4 ] The presence of antiadrenal autoantibodies in serum is characteristic of autoimmune Addison disease (ie, chronic primary adrenal insufficiency, resulting from autoimmune destruction of the adrenal cortex). (medscape.com)
More than 50% of these patients have high serum antithyroid peroxidase antibody levels, and nearly 50% have overt hypothyroidism. (medscape.com)

Antigen retrieval requirements are usually indicated in your antibody datasheets or in previously published data. (nih.gov)
These antibodies are monovalent, containing only a single antigen binding site. (jacksonimmuno.com)
Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissues were incubated with 5 µg/mL of the primary antibody for 60 minutes at room temperature. (biolegend.com)

Set slides in a staining box and apply prepared secondary antibodies to each slide.Incubate for 1hour at room temp. (nih.gov)
If a slide has multiple sections, divide them into two sets: 1) Single or Dual Primary Anitbody(s) 2) Secondary Antibody Control(s). (nih.gov)
See other anti-rabbit secondary antibodies that can be used with this antibody. (abcam.com)
R&D Systems offers VisUCyte™ HRP Polymer secondary antibodies and staining kits to provide cleaner and faster results. (rndsystems.com)
If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. (vectorlabs.com)
Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. (vectorlabs.com)
Following the second incubation period, the plates were washed twice and then analyzed on a BioPlex200 (Bio-Rad, Hercules, CA). A design-of-experiment (DOE) analysis of primary and secondary incubation times of 15 to 60 min showed that there was less than a 7% difference in antibody titers. (cdc.gov)
4) remodeling stage, characterized by the substitution process of replacement of primary by secondary bone tissue (21 days after tooth extraction, in alveolus of rats) 1 . (bvsalud.org)
More severe disease can result from invasion of the lungs by influenza virus (primary viral pneumonia) or by secondary bacterial pneumonia. (cdc.gov)

Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. (abcam.com)
Studies sug- terized by flow cytometry using anti between the immune response and the gest that HCV inhibits receptor genes in CD3, CD56 and CD16 monoclonal virus replication rate [5] and play a cru- the activation of NK cells [20], and the antibodies. (who.int)

For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween ® 20 at 4°C with gentle shaking, overnight. (cellsignal.com)
Incubate overnight at 4°C in a closed, humidified box. (nih.gov)
primary antibody dilution: 1:1000 incubated overnight at 4C. (novusbio.com)
EBV-LMPI and Cyclin DI were applied to different sections and incubated overnight at 40OC in a humidity chamber. (bvsalud.org)
The calibrators, controls, and patient specimens are incubated overnight with iodine I 125 -labeled 21-hydroxylase. (medscape.com)

Western blot analysis of extracts from Jurkat cells, untreated or calyculin A-treated (0.1 µM for 30 minutes), using Phospho-(Ser) 14-3-3 Binding Motif Antibody. (cellsignal.com)
Western blot analysis of extracts from 293 cells transfected with Bad or Bad Ser112 and/or Ser136 mutant fusion proteins, using Phospho-(Ser) 14-3-3 Binding Motif Antibody (top), Phospho-Bad (Ser136) Antibody #9295 (middle) or Phospho-Bad (Ser112) Antibody #9291 (bottom). (cellsignal.com)
Western Blot: Rac1 Antibody [NB100-91266] - WB on tissue lysates. (novusbio.com)
Western blot of HRP anti-α-Synuclein, 80-96 antibody (clone A15115A). (biolegend.com)
The blot was incubated with 0.2 µg/mL of the primary antibody for 60 minutes at room temperature. (biolegend.com)

Clinicians should consider measuring rabies neutralizing antibody titers after completion of PEP if there is any suspicion for immunocompromise. (cdc.gov)
All animals had detectable neutralizing rabies virus antibody titers after vaccination, but one animal failed to meet the World Organization for Animal Health's definition for adequate vaccination (0.5 IU/ml), and two other animals had low antibody titers (0.56 and 0.6 IU/ml). (cdc.gov)

Immunocytochemistry/ Immunofluorescence: Rac1 Antibody [NB100-91266] - Mouse Brain (cerebral cortex). (novusbio.com)
FabuLights are suggested for labeling primary antibodies to be used in immunofluorescence or histochemical experiments. (jacksonimmuno.com)

The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. (jacksonimmuno.com)
The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems. (vectorlabs.com)
cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)

Immunohistochemistry: Rac1 Antibody [NB100-91266] - Rat spinal cord using Rabbit antibody to RAC1, RAC2 (100-150) at 1:1000 dilution. (novusbio.com)

Therefore, laboratory assessment of these types of antibodies is clinically very useful for the diagnosis of autoimmune adrenal disease (eg, Addison disease). (medscape.com)
Background]Antibodies to 21-hydroxylase are markers of autoimmune Addison disease, whether this condition is occurring alone or in conjunction with other autoimmune endocrine diseases as part of type I or type II autoimmune polyglandular syndrome (APS). (medscape.com)
Patients with autoimmune adrenal insufficiency commonly have antibodies against other endocrine glands, whereas healthy patients rarely do. (medscape.com)

Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Mouse thyroid tissue section stained with Thyroglobulin Antibody (2H11 + 6E1). (novusbio.com)
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. (novusbio.com)
Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. (novusbio.com)
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). (novusbio.com)

Depending on site and exposure, rabies may incubate for many years, and a subtle exposure may be forgotten. (medscape.com)

For example, cuprizone-induced demyelination promotes hyper- and depolarizing shifts of the resting membrane potential of auditory thalamocortical pathway neurons and reduction in action potential firing of primary auditory cortex (A1) neurons 7 . (biorxiv.org)
Primary carnitine deficiency is caused by a deficiency in the plasma membrane carnitine transporter, with urinary carnitine wasting causing systemic carnitine depletion. (medscape.com)

Please refer to primary antibody product webpage for recommended antibody dilution. (cellsignal.com)

The antibody may cross-react with immunoglobulins from other species. (jacksonimmuno.com)
When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. (vectorlabs.com)

This Rac1 antibody was developed against a synthetic peptide from amino acid region 100-150 as a part of human Rac1 conjugated to blue carrier protein. (novusbio.com)

Phospho-(Ser) 14-3-3 Binding Motif Antibody ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-14-3-3 binding motif peptides. (cellsignal.com)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing staining of proteins containing phosphorylated 14-3-3 binding motifs, using Phospho-(Ser) 14-3-3 Binding Motif Antibody. (cellsignal.com)
Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. (genetex.com)
WB analysis of extracts from K562 cells treated with H2O2 using GTX55402 CaMKIV (phospho Thr200) antibody. (genetex.com)
There are currently no references for CaMKIV (phospho Thr200) antibody (GTX55402) . (genetex.com)

Adenocarcinomas of other-than-thyroid origin do not react with this antibody. (novusbio.com)
the higher the temperature (ie, the closer to normal body temperature) at which these antibodies react with the RBC, the greater the hemolysis. (msdmanuals.com)

We analyzed the molecular mechanisms leading to glutamate release from rat primary cultures of RPE cells, under isosmotic conditions. (molvis.org)
Primary cultures of rat RPE cells were preloaded with 1 µCi/ml 3 H-glutamate in Krebs Ringer Bicarbonate (KRB) buffer for 30 min at 37 °C. Cells were rinsed and super-perfused with 1 ml/min KRB for 15 min. (molvis.org)
Cardiac fibroblasts are generally the primary effector cells of fibrosis and have been reported to be partly derived from cardiac endothelial cells through the endothelial to mesenchymal transition (EndMT) process. (dovepress.com)
Microglial cells were isolated from postnatal rat brains and identified with CD11 antibody. (cdc.gov)
Primary cultures were obtained with 70% microglial cell population which could be activated by lipopolysacharide (LPS) to produce NO revealed by labeling of cells with a specific fluorogenic reagent, DAF-2DA. (cdc.gov)
Cells were incubated with SWCNT coated with either fluorescently labeled nitrobenzoxadiazole (NBD)-PS or NBD-phosphatidylcholine (PC) for 2h. (cdc.gov)
material limit the use of primary sorted cells. (lu.se)

This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives. (biolegend.com)

After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. (vectorlabs.com)

Similarly, primary cilia have been implicated in regulation of mTOR signaling, in which Tuberous Sclerosis Complex proteins 1 and 2 (TSC1/2) negatively regulate protein synthesis by inactivating the mTOR complex 1 (mTORC1) at energy limiting states. (springer.com)

This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. (novusbio.com)
Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms. (novusbio.com)

Specific antibodies, if present, bind to the 21-hydroxylase during this time. (medscape.com)

Please check your cell and antibody datasheets for specific conditions that may influence the steps you include, exclude or alter. (nih.gov)

High-grade gliomas are primary brain cancers with unacceptably low and persistent survival rates of 10-16 months for WHO grade 4 gliomas over the last 40 years, despite surgical resection and DNA-damaging chemo-radiotherapy. (nature.com)
IHC staining of HRP anti-α-Synuclein, 80-96 antibody (clone A15115A) on formalin-fixed paraffin-embedded normal (left panel) and Parkinson's disease (right panel) human brain tissues. (biolegend.com)

If R&D Systems' primary antibodies are employed, please refer to the product data sheets to obtain the recommended working dilutions. (rndsystems.com)

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. (novusbio.com)

In a Japanese study, primary systemic carnitine deficiency was estimated to occur in 1 per 40,000 births. (medscape.com)

In the absence of Bmi1, M33, or Phc2, primary embryonic fibroblasts (MEFs) are unable to progress into S phase, undergo premature senescence after only a few passages in culture and show an increased accumulation of the tumor suppressors p16 INK4a , p19 ARF and p15 INK4b [4] , [10] . (plos.org)

Host-mediated primary vaccine failure attributed to previously unrecognized impaired immunity is the most likely explanation for this breakthrough infection. (cdc.gov)

The 21-hydroxylase-specific antibodies are quantitated in the clinical laboratory by using radioimmunoassay (RIA). (medscape.com)

OR - Consider multiple sections to be duplicate samples of the same antibody! (nih.gov)

No. MP-2400) for applications of mouse primary antibodies on mouse tissue. (vectorlabs.com)

Sudden death: Unfortunately, the first clinical manifestation in asymptomatic individuals with primary carnitine deficiency may be sudden death. (medscape.com)

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light. (biolegend.com)

Draw off excess blocking solution and apply prepared primary antibodies. (nih.gov)
Discard antibody solution and rinse each slide with a gentle stream of PBS to remove excess antibody. (nih.gov)
Use enough antibody solution to cover the sample with a slight bubble. (nih.gov)
To complex with primary antibody in solution, use 1:1 weight ratio of Fab:primary antibody (3:1 molar ratio). (jacksonimmuno.com)

Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. (jacksonimmuno.com)

Vortex and incubate for 30 minutes at room temperature prior to use. (jacksonimmuno.com)

Incubate at room temp for 1 hr. (nih.gov)

These antibodies may be present even before any reduction in endocrine function is apparent. (medscape.com)

The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions. (biolegend.com)

However, low carnitine levels in newborns may also reflect maternal primary carnitine deficiency. (medscape.com)

Each lot of this antibody is quality control tested by Western blotting . (biolegend.com)

In order to abate the mortality and morbidity of undiagnosed primary carnitine deficiency, this condition has been included in the expanded newborn screening program in several states within the United States. (medscape.com)
4] Primary carnitine deficiency can be identified in infants by expanded newborn screening using tandem mass spectrometry. (medscape.com)

This Rac1 antibody is validated for ICC/IF from a verified customer review. (novusbio.com)

Anatomy20

Organisms20

Diseases12

Chemicals and Drugs111

Analytical, Diagnostic and Therapeutic Techniques and Equipment55

Phenomena and Processes42

Disciplines and Occupations2

Information Science3

Health Care5

Serum8

Antigen3

Secondary9

Monoclonal3

Overnight5

Blot5

Titers2

Immunofluorescence2

Antigens3

Rabbit1

Autoimmune3

Immunohistochemistry4

Rabies1

Membrane2

Dilution1

Species2

Synthetic peptide1

Phospho5

React2

Cells7

Glycerol1

Reagent1

Proteins1

Thyroid2

Bind1

Cell1

Brain2

Product1

Protein1

Occur1

Fibroblasts1

Host1

Specific1

Sections1

Mouse1

Clinical1

Exposure1

Solution4

Remove1

Minutes1

Room1

Present1

Conditions1

Levels1

Control1

Deficiency2

Review1