Incorporation recombinant fusion proteins. Medical search. Frequent questions

This includes (A) engineered protein A and protein G for purification of antibodies (B) affinity tags for purification of recombinant fusion proteins (C) Affibodies - clinically validated protein scaffold binders (D) the first solid phase methods for DNA handling using the biotin - streptavidin system and (E) MabSelect SuRe - alkali-stabled matrix for purification of antibodies. (wikipedia.org)
Weber, Eva Guth, Christina Weiss, Ingrid M. . GFP facilitates native purification of recombinant perlucin derivatives and delays the precipitation of calcium carbonate. (leibniz-inm.de)

The recent approvals of Luxturna® and Zolgensma® prove that recombinant adeno-associated virus (rAAV)-based gene therapy is a transformative modality that enables curative treatment for genetic disorders. (madridistebi.com)
The present invention is in the field of recombinant adeno-associated virus vectors. (justia.com)

Large polypeptides of the Bacillus anthracis protective antigen (PA) were inserted into an influenza A virus hemagglutinin glycoprotein (HA), and the chimeric proteins were functionally characterized and incorporated into infectious influenza viruses. (nih.gov)
Recombinant Vesicular Stomatitis Virus (VSV) can be generated with complementary DNA from complete negative-stranded genomic RNA, with deleted G glycoprotein and, instead, incorporation of other fusion protein, like SARS-CoV-2 Spike (S protein). (madridistebi.com)
Using an antiserum prepared against a synthetic proliferin fusion protein, we show that proliferin is secreted as a glycoprotein by minced placental tissue and that it differs from mouse placental lactogen. (nih.gov)
Paramyxovirus glycoprotein incorporation, assembly and budding: a three way dance for infectious particle production. (microbiologyresearch.org)
The primary envelope glycoprotein gp64 is the most abundant envelope protein and is essential for both insect cell infection and mammalian cell transduction [ 9 ]. (springeropen.com)

The chimeric proteins, designated as LEF/HA (90 amino acid insertion) and RBD/HA (140 amino acid insertion), were initially analyzed following expression using recombinant vaccinia viruses. (nih.gov)
Both chimeric proteins were shown to display functional phenotypes similar to that of the wild-type HA. (nih.gov)
Scientists report on the development of engineered silkworms that express a synthetic spider silk protein and stably produce chimeric silk fibers that are stronger than parental silkworm silk fibers and as tough as spider dragline silk. (genengnews.com)
Following initial ex vivo tests to confirm that the chimeric fusion protein was expressed in posterior silk glands, the piggyBac vector was mixed with a plasmid encoding the piggyBac transposase and injected into eggs isolated from a strain of the B. mori silk moth that has a melaninization deficiency to facilitate detection of the EGFP-tagged chimeric silkworm/spider silk protein in transformed larvae. (genengnews.com)
Further analayses of silk extracted from the cocoons confirmed that at least some of the chimeric silkworm/spidersilk proteins expressed by the transgenic larvae were stably incorporated into the composite silk fibers. (genengnews.com)
Importantly, the team states, although the mechanical properties of the composite silks was variable, fibers containing either EGFP-tagged or untagged chimeric silkworm/spider silk proteins were generally much tougher than parental silkworm fibers and as tough as native dragline spider silk fibers tested under the same conditions. (genengnews.com)
Thus, these results demonstrate that the incorporation of chimeric silkworm/spider silk proteins can significantly improve the mechanical properties of composite silk fibers produced using the transgenic silkworm platform. (genengnews.com)
This recombinant chimeric protein is produced by fusing MAGE-3 with a lipidated protein D derived from H. influenzae at its N-terminus and a sequence of several histidine residues at its C-terminus. (blogspot.com)

WO0153467 provides recombinant yellow fever virus (YFV), which comprise exogenous nucleotide sequences. (allindianpatents.com)
The present invention further provides recombinant AAV (rAAV) virions that comprise a mutant capsid protein. (justia.com)

Eder, Magdalena Koch, Marcus Muth, Christina Rutz, Angela K. Weiss, Ingrid M. . In vivo modified organic matrix for testing biomineralization-related protein functions in differentiated Dictyostelium on calcite. (leibniz-inm.de)
When studying the way in which intermediate filaments assemble in vivo, it is important to distinguish between the incorporation of intermediate filament proteins into an existing intermediate filament network and the ability to form a new network within cells. (ox.ac.uk)
The in vivo incorporation of unnatural amino acids into proteins is a well-established technique requiring an orthogonal tRNA/aminoacyl-tRNA synthetase pair specific for the unnatural amino acid that is incorporated at a position encoded by a TAG amber codon. (nih.gov)
In vivo, recombinant sortilin administration induced arterial hypertension in WT mice. (madridistebi.com)
Depending on the therapeutic application, a drug protein must have rapid or slow in vivo kinetic properties. (wiley.com)
Furthermore, in vivo analysis in a lung metastasis tumor mouse model revealed a superior antitumor effect for scFv_RD_IL-15 in comparison with that obtained by an untargeted or RD missing version of IL-15 fusion protein. (aacrjournals.org)
Mutations that weaken protein-DNA interactions strongly disrupt both condensate formation and DSBs in vivo, and thus these processes are highly correlated. (nature.com)

Such vectors may be advantageous as they preclude the incorporation of heterologous membrane proteins into the viral vector particles. (wur.nl)
In 1989 a recombinant AAV2 (rAAV) gene delivery vector system was first generated, and vectors based on AAV have subsequently been shown to offer numerous major advantages. (justia.com)

The SARS-CoV-2 S protein enables viral entry into host cells and is the primary target for neutralizing antibodies . (news-medical.net)
The most promising approaches are based on antibodies specific for the relatively conserved ectodomain of matrix protein 2 and the intersubunit region of hemagglutinin. (cdc.gov)
While antibodies to the immunodominant, but highly variable, regions of HA and NA can provide potent virus strain-specific protection, the existence of weaker and more broadly protective immune activities directed to less variable regions of viral proteins has long been known ( 5 ). (cdc.gov)
The invention also relates to proteins encoded by such nucleic acid molecules as well as antibodies capable of recognizing these proteins. (allindianpatents.com)
His research focuses on the applications of NMR in the higher-order structural characterization of antibodies and fusion proteins. (chi-peptalk.com)

After cleavage in a Golgi compartment, oligomeric env complexes are transported to the surface of infected cells, where incorporation into budding virions can occur. (nih.gov)
They could not observe fusion of the virions with the cell plasma membrane. (medscape.com)
Moreover, no envelope proteins were seen on the cell plasma membrane at any time during internalization, and the internalized virions were still surrounded by their envelopes. (medscape.com)

Dr Uhlen was the Founding Director of the national infrastructure Science for Life Laboratory (SciLifeLab) from 2010-2015 His group was the first to describe a number of innovations in science including: This broad concept of Affinity-based protein engineering was developed to use specific binding (affinity) of proteins in combination with protein engineering and it has led to many successful applications widely used in the life science community. (wikipedia.org)
Affibody molecules are a class of engineered affinity proteins with proven potential for therapeutic, diagnostic and biotechnological applications. (wiley.com)
Affibody molecules are small (6.5 kDa) single domain proteins that can be isolated for high affinity and specificity to any given protein target. (wiley.com)
Affinity proteins are invaluable tools in the development of next generation therapeutics. (wiley.com)
When expressed as recombinant full-length Abs, a subset of these novel Abs exhibited the binding profiles of QtAbs, as they either failed to bind to monomeric Env protein or showed much higher affinity for Env trimers and VLPs. (plos.org)

Background information: Eukaryotic cilia and flagella are sophisticated organelles composed of several hundreds of proteins that need to be incorporated at the right time and the right place during assembly.Results: Two methods were used to investigate this process in the model protist Trypanosoma brucei: inducible expression of epitope-tagged labelled proteins and fluorescence recovery after photobleaching of fluorescent fusion proteins. (hal.science)
A sequence encoding the green fluorescent protein (GFP) was inserted as reporter gene, and an erythromycin resistance gene was added as selective marker. (biomedcentral.com)
The transformed bacteria expressed on its cell wall surface different fluorescent proteins used as reporters of promoter function. (biomedcentral.com)

Virion-associated env, obtained by crosslinking and detergent extraction, and non-crosslinked secreted env ectodomain (recombinant gp140) were purified by lentil-lectin chromatography and gel filtration as single predominant species. (nih.gov)
These QtAbs bound pre-fusion forms of antigen and showed differential binding kinetics depending on whether oligomers were produced as recombinant gp140 trimers or as full-length Env incorporated into VLPs. (plos.org)

A recombinant fusion protein derived from the melanoma antigen MAGE-3 with potential immunostimulating and antineoplastic activities. (blogspot.com)

The invention relates to dengue virus (DEN) and in particular to a recombinant virus that is prepared by replacing DEN structural protein genes with non-dengue transgenes, such as, therapeutic antigens, and its production and application. (allindianpatents.com)
This work supported both small-molecule high-throughput screening and protein therapeutic efforts. (chi-peptalk.com)
Subsequently, he worked as a postdoctoral researcher in the group of Prof. T.P.J. Knowles at the Department of Chemistry at the University of Cambridge, U.K. His research interests include the development, characterization and formulation of therapeutic proteins. (chi-peptalk.com)

However, the zoonotic potential of NDV raises safety concerns regarding the use of live NDV recombinants, as the incorporation of a heterologous attachment protein may result in the generation of NDV with altered tropism and/or pathogenicity. (wur.nl)
This study thus provides proof of concept for the use of recombinant vector vaccines expressing a soluble form of a heterologous viral membrane protein. (wur.nl)

In their attempt to generate transgenic silkworms that produced spider-silk proteins in their silk, the team designed a piggyBac vector with specific features. (genengnews.com)
The vector encoded the synthetic spider silk protein A2S814, which exhibits both elastic and strength motifs, along with the silkmoth Bombyx mori fibroin heavy chain (fhc) protein, to target spider silk protein production specifically to the silkworm's posterior silk gland, and an fhc enhancer to increase expression levels. (genengnews.com)
8. A method for producing a Staphylococcus epidermidis polypeptide comprising culturing a cell comprising a recombinant expression vector comprising a nucleic acid encoding SEQ ID NO: 5314 under conditions that permit expression of the polypeptide. (patentsencyclopedia.com)
An expression cassette was assembled onto the pCR2.1-Topo vector by cloning the promoter, leader peptide, cell wall anchor and terminator sequences derived from the laminin binding S-layer protein gene of L. crispatus strain F5.7 ( lbs gene). (biomedcentral.com)
Most of the SARS-CoV-2-based vaccines are based on the full spike protein, which is presented as a trimerized protein, and many viral vector vaccines express the spike protein into the host cells and do not display it on virus surfaces. (springeropen.com)
Thus, we evaluated the immunoprotection raised by co-immunization with Ad5-gp83 and an Ad vector containing an epitope (ASP-M) of the T. cruzi amastigote surface protein 2. (paijournal.com)
Protein IX (pIX)-modified Ad vector (Ad5-pIX-ASP-M) was generated, characterized, and validated. (paijournal.com)

Electrophoretic mobility shift assay established the binding of these homeobox proteins with specific DNA sequences, AH1 (CAAT(A/T)ATTG) and AH2 (CAAT(C/G)ATTG). (frontiersin.org)
Conformational epitopes within mature Env can be found within a single protomer, and distant patches of amino acid sequences that are brought together by protein folding. (plos.org)
The present invention further provides nucleic acids comprising nucleotide sequences that encode mutant capsid proteins, and host cells comprising the nucleic acids. (justia.com)

A recent International Journal of Molecular Sciences study reports that the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) recombinant lineage XD, otherwise known as 'Deltacron,' does not exhibit higher transmissibility or immune evasion than the Omicron BA.1 variant. (news-medical.net)
Here, we describe a novel protocol for incorporating unnatural amino acids into fully deuterated proteins using glucose-based media (which are relevant to the production, for example, of amino acid-specific methyl-labeled proteins used in the study of large molecular weight systems). (nih.gov)
Here we address this through the molecular characterization of Saccharomyces cerevisiae RMM (Rec114, Mei4 and Mer2) proteins-essential, conserved components of the DSB machinery 2 . (nature.com)
These findings provide insight into the molecular basis for human antibody recognition of paramyxovirus surface proteins and the mechanisms of SOSV neutralization. (cdc.gov)
He is trained in the fields of protein engineering, molecular biology and structural biology at University of Toronto and Stanford University and Queen's University. (chi-peptalk.com)

Type III modules are present in both extracellular and intracellular proteins. (embl.de)
Each domain consists of two antiparallel beta sheets and is folded topologically identically to single fibronectin type III domains from the extracellular matrix proteins tenascin and fibronectin. (embl.de)
Arabinogalactan proteins (AGPs) are abundant extracellular proteoglycans that are found in most plant species and involved in many cellular processes, such as cell proliferation and survival, pattern formation, and growth, and in plant microbe interaction. (hindawi.com)

Network incorporation of intermediate filament molecules differs between preexisting and newly assembling filaments. (ox.ac.uk)
Although recent advances have driven significant progress in predicting three-dimensional protein structures from sequence, proteins are not static molecules. (stanford.edu)

E. Coli is present in the human digestive system and is known to accelerate stable isotope enrichment of amino acids with 13 C, 15 N, and/or 17 O. Thereby acesulfame-K and aspartame induce isotopic enrichments and excessive 13 C, 15 N, and/or 17 O introductions into proteins and nucleic acids. (ej-physics.org)

2. DT388-GM-CSF, a novel fusion toxin consisting of a truncated diphtheria toxin fused to human granulocyte-macrophage colony-stimulating factor, prolongs host survival in a SCID mouse model of acute myeloid leukemia. (nih.gov)
3. Modulation of the apoptotic response of human myeloid leukemia cells to a diphtheria toxin granulocyte-macrophage colony-stimulating factor fusion protein. (nih.gov)
6. Toxicology and pharmacokinetics of DT388-GM-CSF, a fusion toxin consisting of a truncated diphtheria toxin (DT388) linked to human granulocyte-macrophage colony-stimulating factor (GM-CSF) in C57BL/6 mice. (nih.gov)
8. Variable cytotoxicity of diphtheria toxin 388-granulocyte-macrophage colony-stimulating factor fusion protein for acute myelogenous leukemia stem cells. (nih.gov)
9. Malignant progenitors from patients with acute myelogenous leukemia are sensitive to a diphtheria toxin-granulocyte-macrophage colony-stimulating factor fusion protein. (nih.gov)
11. Acute myeloid leukemia cells in G0 phase of the cell cycle that are unresponsive to conventional chemotherapy are sensitive to treatment with granulocyte-macrophage colony-stimulating factor/diphtheria toxin fusion proteins. (nih.gov)
12. Phase I trial of a novel diphtheria toxin/granulocyte macrophage colony-stimulating factor fusion protein (DT388GMCSF) for refractory or relapsed acute myeloid leukemia. (nih.gov)
A recombinant fusion protein composed of the diphtheria toxin with the receptor-binding domain replaced by human epidermal growth factor (EGF). (blogspot.com)
After binding to the EGF receptor, the agent is internalized by the cell, where the diphtheria toxin moiety exerts its cytotoxic effect, inhibiting protein synthesis through ADP-ribosylation of elongation factor 2. (blogspot.com)

In this study, we developed reverse genetics systems, including minigenomes and recombinant viruses, that will facilitate the study of these pathogens. (cdc.gov)
As a proof-of-concept study, we used both minigenomes and recombinant viruses to test the inhibitory effects of previously reported antiviral compounds. (cdc.gov)
The produced recombinant viruses can induce the immune response without adjuvant, which needs dose optimization and further stability tests. (springeropen.com)

He has over 30 peer-reviewed publications in the area of protein formulation, biophysical characterization and structural biology. (chi-peptalk.com)

Participating scientists gathered for one and one half days to focus on methods and techniques for the high throughput production, purification and crystallization of target proteins. (nih.gov)
Auto-inducing media have also been developed for labeling proteins with selenomethionine, for enriching proteins with 15N and 13C, and for production of target proteins by arabinose induction of T7 RNA polymerase from the pBAD promoter in BL21-AI. (nih.gov)
Therefore, current protein knockdown techniques, directly target proteins of interest (POIs) with the cellular ubiquitin-proteasome system (UPS), mainly using two strategies. (nature.com)
The goal of this study is to develop versatile toolsets for efficient and controlled depletion of tagged as well as untagged endogenous target proteins in cells and organisms. (nature.com)
As a general strategy we replaced the native substrate binding domain of E3 ubiquitin ligases with recombinant binding modules to direct the ubiquitin ligase activity to selected target proteins and mark them for depletion. (nature.com)

The first gene encodes four proteins necessary for genome replication (Rep78, Rep68, Rep52, and Rep40), and the second expresses three structural proteins (VP1-3) that assemble to form the viral capsid. (justia.com)

The application of this pseudovirus has been addressed by its use in neutralizing antibody assays in order to evaluate a new vaccine, emergent SARS-CoV-2 variants (delta and omicron), and approved vaccine efficacy against variants of concern as well as in viral fusion-focused treatment analysis that can be performed under BSL-2 conditions. (madridistebi.com)
Recognized by specific cytotoxic T lymphocytes, D1/3-MAGE-3-His fusion protein may boost antitumoral immune responses when used in a vaccine formulation. (blogspot.com)

Although this technology provides unique opportunities to engineer protein structures, poor protein yields are usually obtained in deuterated media, hampering its application in the protein NMR field. (nih.gov)

S protein-coding expression plasmids were transfected into 293T cells plated in six-well plates. (news-medical.net)
Our protocol results in high level of isotopic incorporation (~95%) and retains the high expression level of the target protein observed in Luria-Bertani medium. (nih.gov)
16. High-level expression and purification of the recombinant diphtheria fusion toxin DTGM for PHASE I clinical trials. (nih.gov)
Expression of these proteins is spatially and temporally regulated during embryonic development. (cancerindex.org)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
Activation of pathways in recipient cells was determined at gene and protein expression levels. (cancerindex.org)
Infection of a host cell with a recombinant YFV provides for expression of the exogenous nucleic acid in a host cell and production of an antigenic polypeptide encoded by the exogenous nucleic acid. (allindianpatents.com)
Expression of the matrix (M) protein triggered release of enveloped, matrix-containing virus-like particles (VLPs) from cells. (microbiologyresearch.org)
Additionally, expression of N protein enabled incorporation of the phosphoprotein (P) into VLPs, likely due to known N-P interactions. (microbiologyresearch.org)
C ell-free protein expression is a rapid and high-throughput methodology for conver- sion of DNA-encoded genetic information into protein-mediated biochemical activities. (pdfroom.com)
Rich Altman has 30 years of experience in protein expression and production. (chi-peptalk.com)

This concept involves the detecting of the incorporation of nucleotides in real-time during synthesis by a DNA polymerase and to use this for DNA sequencing. (wikipedia.org)
As part of our investigation of the possible role of proliferin in cell proliferation, we wanted to determine the site of synthesis of this protein in the intact animal by screening a series of mouse organs and tissues for the presence of proliferin mRNA. (nih.gov)
The Sendai virus nonstructural C proteins specifically inhibit viral mRNA synthesis. (microbiologyresearch.org)
Much bio- technological progress in the last 40 years has been focused on developing more efﬁ cient analysis and synthesis technologies for both DNA and proteins. (pdfroom.com)
However, while orders of magnitude reduction in costs for DNA sequencing and synthesis was achieved during the last decade, the throughput and cost of technologies for protein production and engineer- ing have changed comparatively little. (pdfroom.com)

The Omicron variant harbors over 30 mutations in the S protein, which alter cell tropism and the transmission potential of SARS-CoV-2 while significantly decreasing antibody-mediated neutralization. (news-medical.net)
Quantitatively mapping the physical parameters that dictate these landscapes and protein stability is therefore critical to develop models that are capable of predicting how mutations alter function of proteins in disease and informing the design of proteins with desired functions. (stanford.edu)
B-raf belongs to the the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which may be constitutively activated due to BRAF gene mutations. (blogspot.com)

Folding a linear chain of amino acids into a three-dimensional protein is a complex physical process that ultimately confers an impressive range of diverse functions. (stanford.edu)
or conjugate, complex, or fusion protein or fusion polypeptide including the same disclosed amino acid sequence derived from bacterium (e.g., mycoplasma, anaplasma, etc. (patentsencyclopedia.com)

and tDepartment of Biology, University of California, Santa Cruz, CA 95064 Contributed by Daniel Nathans, March 15, 1985 ABSTRACT Proliferin is a recently described, prolactin- related protein whose mRNA appears in several murine cell lines during active growth. (nih.gov)

These findings reinforce the central importance of the M protein in virus assembly and release, but also illustrate the variable roles of other paramyxovirus proteins during these processes. (microbiologyresearch.org)
Paramyxovirus Sendai virus C proteins are essential for maintenance of negative-sense RNA genome in virus particles. (microbiologyresearch.org)

The ultimate aim for the project is a complete understanding of the functions and interactions of all proteins and where in the different cells and tissues they reside. (wikipedia.org)
The results confirm the importance of the coiled coil interactions in determining the fate of intermediate filament proteins. (ox.ac.uk)
and (iii) protein-protein interactions. (hindawi.com)
FLIM-FRET analysis of protein-protein interactions showed that PLIN5 S155 phosphorylation regulates PLIN5 interaction with adipose triglyceride lipase at the lipid droplet, but not with α-β hydrolase domain-containing 5. (uci.edu)

Soluble human ACE2-Fc constructs and flow cytometry were used to determine the ability of XD S protein to bind ACE2. (news-medical.net)
reported that the human CD46 molecule is a receptor for HHV-6, based on observations that CD46 is downregulated by HHV-6 infection, that HHV-6 infection is inhibited by an anti-CD46 antibody or by soluble CD46, and that nonhuman cells that express recombinant human CD46 become susceptible to HHV-6 infection. (medscape.com)
Therefore, an antibody cytokine fusion protein (scFv_RD_IL-15) composed of an antibody moiety targeting the tumor stromal fibroblast activation protein (FAP), an extended IL-15Rαsushi domain (RD) and IL-15 was generated, exhibiting antibody-mediated specific binding and cytokine activity in soluble and targeted form. (aacrjournals.org)
Methodology/Principal Findings: In the present study we generated recombinant NDVs expressing either full length, membrane-anchored HA of the H5 subtype (NDV-H5) or a soluble trimeric form thereof (NDV-sH5 3). (wur.nl)

His research is focused on protein science, antibody engineering and precision medicine and range from basic research in human and microbial biology to more applied research, including clinical applications. (wikipedia.org)
The objective of the program is to map all the human proteins in cells, tissues, and organs using integration of various omics technologies, including antibody-based imaging, mass spectrometry-based proteomics, transcriptomics, and systems biology. (wikipedia.org)
The XD recombinant strain has raised concerns, as it may combine the Delta variant's increased pathogenicity with the Omicron VOC's high transmissibility and antibody evasion properties. (news-medical.net)
The study tested S proteins from the recombinant SARS-CoV-2 XD lineage and the Delta and Omicron VOCs for their capacity to bind with the angiotensin-converting enzyme-2 (ACE-2) receptor to promote virus-cell and cell-cell fusion, as well as resistance to antibody-mediated neutralization. (news-medical.net)
Targeted approaches by means of antibody-cytokine fusion proteins might enable focus on the cytokine activity to the tumor site, thereby reducing unwanted side effects. (aacrjournals.org)
Comparative analysis with a corresponding antibody fusion protein devoid of RD (scFv_IL-15) showed for scFv_RD_IL-15 in solution enhanced stimulatory activity on Mo7e (IL-15Rβγ) cells and reduced proliferation response on CTLL-2 (IL-15Rαβγ) cells, while in FAP-targeted, that is, membrane-bound form, comparable proliferation of CTLL-2 (IL-15Rαβγ) cells was obtained. (aacrjournals.org)
Thus, tumor-directed trans -presentation of IL-15 in association with RD in form of an antibody fusion protein seems to be a promising approach to further improve the antitumor effect of IL-15. (aacrjournals.org)
Dr. Jarrett Adams is a research associate at the University of Toronto and the Associate Director of the Toronto Recombinant Antibody Centre. (chi-peptalk.com)

The recombinant full-length and truncated homeobox proteins were found to be localized in the nucleus. (frontiersin.org)
Altogether, our results suggest the possible role of OsHOX22/OsHOX24 homeobox proteins as negative regulators in abiotic stress responses. (frontiersin.org)

Perilipin 5 (PLIN5) is a lipid-droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and is thought to regulate lipid metabolism in response to phosphorylation by protein kinase A (PKA). (uci.edu)

We recently cloned a gene from differentiating thymocytes that encodes a member of the Ig superfamily of cell surface proteins ( 3 ). (aai.org)

10. Cell-specific modulation of drug resistance in acute myeloid leukemic blasts by diphtheria fusion toxin, DT388-GMCSF. (nih.gov)

Daunert's strategies involve the design and preparation of biosensing systems based on bacterial whole cells and proteins that are genetically engineered to respond to the analytes of interest with the production of a detectable signal (e.g., fluorescence, bioluminescence, chemiluminescence). (nih.gov)
Replacement of the head domain with the bovine K19 sequence did not prevent the incorporation of K19GFAP into the existing network of vimentin intermediate filaments in NIH 3T3 cells but it was incompatible with de novo formation of filament networks in the epithelial cell line MCF-7, which lacks an endogenous vimentin network. (ox.ac.uk)
Because of its similarity to prolactin and its association with proliferating cells, the encoded mature protein was called proliferin or PLF. (nih.gov)
These findings suggest HHV-6 enters its target cells through the endocytic pathway and not by fusion with the plasma membrane. (medscape.com)
Initially discovered as a widely expressed C3b- and C4b-binding protein, CD46 was subsequently shown to act as a cofactor for serine protease factor I to inactivate C3b and C4b, which are opsonins and components of convertases, by limited proteolysis, thereby protecting host cells from damage by complement factors. (medscape.com)
To try and get around this problem recombinant spider silk proteins have been produced in hosts such as bacteria, yeast, baculovirus/insect systems, mammalian cells, and transgenic plants and animals. (genengnews.com)
We have developed versatile tools based on recombinant E3 ubiquitin ligases that are controlled by light or drug induced heterodimerization for nanobody or DARPin targeted depletion of endogenous proteins in cells and organisms. (nature.com)
DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. (uci.edu)
tive drug target because the CD4 binding site of gp120 is well phy and atomic force microscopy to characterize the structure of conserved and because de novo infection of target cells would be an extremely potent HIV-neutralizing protein, D1D2-Ig tp blocked, preventing all subsequent stages of the viral life cycle. (nih.gov)
This protein, independently isolated from Th1 cells, has been named B and T lymphocyte attenuator (BTLA) 3 ( 4 ). (aai.org)
To understand the roles of human parainfluenza virus 3 (HPIV3) proteins in assembly and release, viral proteins were expressed individually and in combination in 293T cells. (microbiologyresearch.org)

The SARS-COV-2 delta-omicron recombinant lineage (XD) exhibits immune-escape properties similar to the Omicron (BA.1) variant. (news-medical.net)
Such recombinant YFV are useful in eliciting an immune response to the exogenous polypeptide. (allindianpatents.com)
The outcome of our investigation showed that the monomeric RBD proteins were properly displayed on baculovirus and efficiently triggered the mouse immune system. (springeropen.com)

However, the spike (S) protein open reading frame has been replaced with a sequence matching the Omicron sublineage BA.1. (news-medical.net)
The nucleotide sequence of the PLF-1 cDNA revealed a single open reading frame encoding a protein of about M, 24,000 with clear homology to preprolactin (2). (nih.gov)
Generally, it is thought that Env epitopes that induce broadly neutralizing Abs are difficult to access and poorly immunogenic because of the characteristic oligomerization, conformational flexibility, sequence diversity and extensive glycosylation of Env protein. (plos.org)
The synthetic spider silk sequence was flanked by the N- and C-terminal domains of the B. mori fhc protein. (genengnews.com)
CAAT box (also CAT box) A conserved sequence found within the promoter region of the protein-encoding genes of many eukaryotic organisms. (fao.org)
Here, we review the approaches that are used to quantify protein stability at a variety of scales, from returning multiple thermodynamic and kinetic measurements for a single protein sequence to yielding indirect insights into folding across a vast sequence space. (stanford.edu)

Centromere protein (CENP) A, a histone H3 variant, is a key epigenetic determinant of chromosome domains known as centromeres. (elifesciences.org)

During the initial pilot phase of the PSI research centers have made a significant progress in establishing high throughput protein structure determination pipelines based on x-ray crystallography and NMR. (nih.gov)

Recently several infections caused by a recombinant SARS-CoV-2 lineage containing genomic portions of the Delta and Omicron variants have been reported. (news-medical.net)
Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild. (stanford.edu)
Miniaturization aims to extract the most information out of the smallest amount of the largest possible number of proteins or protein variants at the lowest possible cost. (pdfroom.com)

B and T lymphocyte attenuator (BTLA) is a recently identified inhibitory Ig superfamily cell surface protein found on lymphocytes and APC. (aai.org)
The cytoplasmic tail of BTLA contains ITIM motifs, suggesting an inhibitory function for the protein. (aai.org)

and it is one of several sites for recognition and binding of regulatory proteins called transcription factors. (fao.org)
Recently, the auxin-induced protein degradation system was generalized by combining the auxin-induced degron (AID) and GFP binding nanobody to degrade GFP fusion protein controllably 15 . (nature.com)
For ubiquitination, proteins are recognized and bound by the substrate binding part of specific E3 ubiquitin ligases, which ligate ubiquitin to the substrate proteins via their catalytic domain. (nature.com)
The incorporation of d-lactate into peptidoglycan precursors leads to the increased loss of among the five hydrogen bonds, resulting in a 1,000-fold reduction in vancomycin antibiotic activity, while reduced binding of vancomycin towards the d-Ala-d-Ser termini of lipid II is because Fadrozole of steric hindrance (12,C14). (insulin-receptor.info)
The anti-HN mAbs all target the globular head of the HN protein and can be organized into 4 competition-binding groups that exhibit epitope diversity. (cdc.gov)
Here, we report that the removal of the special AT-rich binding protein 2 (SATB2), a nuclear protein known to bind matrix attachment regions, is a key event in initiating myogenic differentiation. (mdpi.com)

The double-stranded DNA Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the prototype of baculoviruses that has been used extensively to produce eukaryotic complex proteins [ 5 ]. (springeropen.com)

The envelope proteins (env) of simian immunodeficiency virus (SIV) and HIV type 1 assemble to form noncovalently associated oligomers in the endoplasmic reticulum. (nih.gov)

Identifying structural determinants of human immunodefi- protein gp120 to the cell surface receptor CD4 (1-3). (nih.gov)

KLF4 knockdown promoted myoblast proliferation and inhibited myoblast fusion, while its overexpression showed opposite results. (biomed.news)

We use this rapid, tunable and reversible protein depletion for functional studies of essential proteins like PCNA in DNA repair and to investigate the role of CED-3 in apoptosis during Caenorhabditis elegans development. (nature.com)
The cellular abundance of proteins is determined by transcription, translation and degradation, each of which may be targeted for functional studies. (nature.com)
We isolated six mAbs recognizing the functional attachment protein hemagglutinin-neuraminidase (HN) and 18 mAbs against the fusion (F) protein. (cdc.gov)

MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)
Recruitment of Alix/AIP1 to the plasma membrane by Sendai virus C protein facilitates budding of virus-like particles. (microbiologyresearch.org)

Our findings indicate that mitotic chromosomes in general and ribosomal genes in particular, although highly condensed, are accessible to transcription factors and chromatin proteins. (rupress.org)

USA Vol. 82, pp. 4356-4359, July 1985 Biochemistry Identification of proliferin mRNA and protein in mouse placenta (placental hormone / placental lactogen/prolactin-related protein) DanIEL I. H. Linzer*t, Se-Jin Leg*, LINDA OGREN? (nih.gov)

The accurate segregation of chromosomes during meiosis-which is critical for genome stability across sexual cycles-relies on homologous recombination initiated by DNA double-strand breaks (DSBs) made by the Spo11 protein 1 , 2 . (nature.com)

To distinguish between these alternatives, we have made a hybrid construct consisting of the rod and tail domains of murine glial fibrillary acidic protein (GFAP) coupled to the head domain of bovine keratin 19, called K19GFAP. (ox.ac.uk)
These independent tools can be combined for spatial and temporal depletion of different sets of proteins, can help to distinguish immediate cellular responses from long-term adaptation effects and can facilitate the exploration of complex networks. (nature.com)

In contrast, the membrane-associated protein arginine kinase 3 (AK3) showed rapid turnover in both growing and mature flagella, without particular polarity and independently of intraflagellar transport.Conclusion: These results demonstrate different modes of incorporation for structural and membrane-associated proteins in flagella.Significance: The existence of two distinct modes for incorporation of proteins in growing flagella suggests the existence of different targeting machineries. (hal.science)

Engineered larvae spin composite fibers containing synthetic spider silk protein. (genengnews.com)
It holds the promise to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. (pdfroom.com)

Besides this, HD-ZIP II proteins contain ZIBEL and CE motifs, whereas HD-ZIP III proteins contain MEKHLA domain also. (frontiersin.org)

To this end, the researchers used vesicular stomatitis pseudovirus particles containing the corresponding S protein as a surrogate system for researching SARS-CoV-2 host cell entrance and neutralization. (news-medical.net)

Fig. 1: Purification and subunit arrangement of the S. cerevisiae RMM proteins. (nature.com)

We also investigated the ability of an appropriate type II keratin partner to rescue K19GFAP from incorporation into a vimentin network and initiate de novo filament assembly, using the fibroblast cell line KF-K8(3), an NIH 3T3 fibroblast cell line expressing a single human keratin, K8. (ox.ac.uk)
Next, the S protein-driven cell-cell fusion that contributes to COVID-19 pathogenesis was analyzed. (news-medical.net)
The lectures will cover the current status of cell-based protein production systems and the theoretical aspects of the methodology. (lu.se)
Overall, BTLA adds to the growing list of cell surface proteins that are potential targets to down-modulate T cell function. (aai.org)

BACKGROUND OF THE INVENTION The soybean, Glycine max (L.) Merril (Glycine max or soybean), is one of the major economic crops grown worldwide as a primary source of vegetable oil and protein (Sinclair et al. (allindianpatents.com)

Anatomy13

Organisms14

Diseases1

Chemicals and Drugs52

Analytical, Diagnostic and Therapeutic Techniques and Equipment19

Phenomena and Processes48

Technology, Industry, Agriculture1

Information Science3

Health Care1

Purification of recombinant2

Adeno-assoc2

Glycoprotein5

Chimeric8

Provides recombinant2

Vivo7

Vectors2

Antibodies5

Virions3

Affinity5

Fluorescent3

Gp1402

Antigen1

Therapeutic3

Heterologous2

Vector7

Sequences3

Molecular5

Extracellular3

Molecules2

Nucleic acids1

Diphtheria toxin9

Viruses3

Characterization1

Target proteins5

Structural proteins1

Vaccine2

Structures1

Expression11

Synthesis5

Mutations3

Amino2

Abstract1

Paramyxovirus2

Interactions4

Soluble4

Antibody8

Homeobox2

Intracellular1

Encodes1

Myeloid1

Cells11

Immune3

Sequence6

Chromosome1

High-throughput1

Variants3

Inhibitory2

Binding6

Eukaryotic1

Envelope1

Gp1201

Knockdown1

Functional3

Plasma membrane2

Transcription factors1

MRNA1

Genome1

Distinguish2

Results demonstrate1

Synthetic2

Motifs1

Particles1

Subunit1

Cell4

Invention1