Incorporation of the stable isotope stable isotope labelling. Medical search. Frequent questions

Isolation and GC-MS analyses of the isotope incorporation into the resultant hyoscyamine does not support the involvement of a vicinal interchange process operating during the isomerisation of littorine to hyoscyamine. (sinapse.ac.uk)
C cycling is investigated under field conditions by help of a triple 13CO2 pulse labeling experiment and subsequent analyses of the isotope label in plant and soil samples. (uzh.ch)

Below lists is the most common stable isotopically labeled amino acids that can be used for the synthesis of peptides with stable isotopes containing carbon 13 ( 13 C), Nitrogen 15 ( 15 N). (novoprolabs.com)
After culture stabilization, [U-13C] glucose was added as an isotopically labeled metabolic precursor. (tno.nl)

Recently, a new technique called NeuCode (neutron encoding) SILAC, has augmented the level of multiplexing achievable with metabolic labeling (up to 4). (wikipedia.org)
Labeling patterns confirmed the metabolic pathways responsible for the biosynthesis of many detected metabolites, and labeling was detected in unexpected metabolites, including two higher sugar phosphates annotated as octulose phosphate and nonulose phosphate. (acs.org)
Stable Isotope Products for Chemical Labeling Stable isotope coded chemical tags enable researchers to perform mass spectrometric- based proteomics studies in the absence of metabolic labeling. (firebaseapp.com)
Stable isotope 13C labeling is an important tool to analyze cellular metabolic flux. (biomed.news)
The correction method was then applied to the intracellular metabolic flux analysis of 13C-labeled Aspergillus niger. (biomed.news)
Metabolic labelling with stable isotopes in combination with quantitative proteomics were used to estimate the rates of incorporation of new proteins into cartilage, bone and skin. (ox.ac.uk)
The purpose of this project is to develop state-of-the-art stable-isotope vibrational microspectroscopy based approach for in vivo monitoring of hyphal metabolic processes among competing ectomycorrhizal and saprotrophic fungi, and to identify nutrient (C and N) conditions which determine 'the winner' of the competition and its influence on organic matter decomposition. (lu.se)
For this, I will first measure the hyphal growth and metabolic activity rates in single fungal strains growing in soil chips by quantifying cellular incorporation of stable-isotope labeled nutrient substrates via analyzing characteristic spectral signatures obtained through vibrational microspectroscopy. (lu.se)
This study introduces a stable-isotope metabolic approach employing [U-13C]glucose that, as a novelty, allows selective profiling of the human intestinal microbial metabolic products of carbohydrate food components, as well as the measurement of the kinetics of their formation pathways, in a single experiment. (tno.nl)
Using computer modeling of the 12C contents and 13C labeling kinetics, the metabolic fluxes in the gut microbial pathways for synthesis of lactate, formate, acetate, and butyrate were determined separately for glucose and unlabeled background substrates. (tno.nl)

Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling. (wikipedia.org)
A SILAC approach involving incorporation of tyrosine labeled with nine carbon-13 atoms (13C) instead of the normal carbon-12 (12C) has been utilized to study tyrosine kinase substrates in signaling pathways. (wikipedia.org)
Pulsed SILAC (pSILAC) is a variation of the SILAC method where the labelled amino acids are added to the growth medium for only a short period of time. (wikipedia.org)
It had also been used to study biofilm tolerance to antibiotics to differentiate tolerant and sensitive subpopulations Traditionally the level of multiplexing in SILAC was limited due to the number of SILAC isotopes available. (wikipedia.org)
The NeuCode amino acid method is similar to SILAC but differs in that the labeling only utilizes heavy amino acids. (wikipedia.org)
The use of only heavy amino acids eliminates the need for 100% incorporation of amino acids needed for SILAC. (wikipedia.org)
According to Josh Coon, professor of chemistry and biomolecular chemistry at UW-Madison and leader of the effort, the new method brings together benefits of SILAC labeling and isobaric tagging. (genomeweb.com)
The method, Coon noted, synthesizes some of the best aspects of isobaric labeling and SILAC, combining, in particular, the multiplexing capabilities of the former and the MS1 level quantitation of the latter. (genomeweb.com)
SILAC - or stable isotope labeling by amino acids in cell culture - uses the incorporation of amino acids containing different isotopes to label proteomic samples for quantitative mass spec analysis. (genomeweb.com)
The research team at the Kennedy Institute used an in vivo technique called pulsed SILAC labelling in healthy mice to show changes in the synthesis of proteins of the matrisome over three stages of life: during maximum skeletal growth, and at young, and older adulthood. (ox.ac.uk)

In contrast, the second population is fed with growth medium containing amino acids labeled with stable (non-radioactive) heavy isotopes. (wikipedia.org)
SIP encompasses a series of methods that involve the incorporation of heavy isotopes into newly synthetized DNA and its separation on a density gradient 16 . (nature.com)

Proteins from both cell populations are combined and analyzed together by mass spectrometry as pairs of chemically identical peptides of different stable-isotope composition can be differentiated in a mass spectrometer owing to their mass difference. (wikipedia.org)
Isobaric labeling uses stable isotope tags attached to peptides of interest to enable relative or absolute quantitation of proteins via tandem mass spectrometry. (genomeweb.com)
Digested peptides are labeled with tags that fragment during MS2 to produce signals corresponding to the amount of peptide present in a sample. (genomeweb.com)
Because these differentially labeled peptides are quantitated at the MS1 level, precursor interference isn't a problem. (genomeweb.com)
Stable isotope labeled peptides, or SIL peptides, maintain identical amino acid sequence with the natural ones released from the parent proteins by chemical or enzymatic cleavage, but some of the amino acid residuals are labeled by specific stable isotope, such as 2 H, 13 C, and/or 15 N atom(s). (novoprolabs.com)
Stable isotope labeled peptides have been widely applied in the nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS). The combination of SIL peptides with NMR spectroscopy allow for the incorporation of NMR active nuclei which can reduce the complexity of spectra, and then help researchers to obtain novel correlations between atoms for more structural information. (novoprolabs.com)
With more than 10 years of experience, and as a world-leading peptide manufacturing organization, NovoPro can provide customized peptides labeled with stable isotopes including 2 H, 15 N & 13 C, respectively, or a combination of 15 N & 13 C. Even when the peptides are attached with specific modification motifs. (novoprolabs.com)
Labels such as isobaric chemical tags with different masses can be introduced through covalent modification to peptides. (openaccesspub.org)
Syntrophic interactions were confirmed by analysis of the time-dependent incorporation of 13 C into peptides, and quantified by calculation of relative isotope abundance (RIA) and labeling ratio (lr) from mass spectral isotope patterns. (jiomics.com)
Mass spectral data indicated that 13 C-labeled organic substances, mostly peptides, secreted by the chemoautotroph were assimilated by the heterotroph. (jiomics.com)

Uniform 15 N labeling results in an isolated spin 2018-06-12 Isotopic labeling is a technique for tracking the passage of a sample of substance through a system. (firebaseapp.com)
The Applications in human mineral Isotopic labeling of compounds is a non-radioactive method of labeling, provides site-specific investigation of structures, makes molecules easily detectable by mass spectrometry and NMR, maintaining the physico-chemical properties of the target molecule, and is cost-effective and easy to use. (firebaseapp.com)
Metabolite concentrations and isotopic labeling were determined by NMR, GC, and enzymatic methods. (tno.nl)

As a new quantitative method, heavy water-labeled single-cell Raman microspectroscopy can reduce damage to cells and human interference factors. (medsci.org)
These MS-based quantitative approaches using label-free, tags, or stable isotope labeling have their own strengths and limitations. (openaccesspub.org)
Quantitative proteomic methods fall into two general categories: label-based or label-free quantification. (openaccesspub.org)

To characterize the relationship of genetic asthma susceptibility with sphingolipid synthesis, we analyzed asthma-associated 17q21 genotypes (rs7216389, rs8076131, rs4065275, rs12603332, and rs8067378) in both children with asthma and those without asthma, quantified plasma and whole-blood sphingolipids, and assessed sphingolipid de novo synthesis in peripheral blood cells by measuring the incorporation of stable isotope-labeled serine (substrate) into sphinganine and sphinganine-1-phosphate. (jci.org)
The synthesis of folic acid, multiply labelled with stable isotopes The isotope-labeled compounds fluoxetine-d5 and norfluoxetine-d5 were used to study matrix effects caused by co-eluting compounds originating from raw and Isotope labeling methods for large systems. (firebaseapp.com)
Despite reduced ecosystem carbon uptake and total VOC emissions, plants continued to allocate a similar proportion of fresh carbon to de novo VOC synthesis, as incorporation of 13C into both isoprene and monoterpenes remained high. (copernicus.org)
The synthesis of the complementary DNA strand starts at the specific priming site and ends with the incorporation of a chain-terminating ddNTP that is randomly introduced instead of its corresponding dNTP . (jenabioscience.com)

Nuclear magnetic resonance spectroscopy, Radiolabeling, Biomolecules-Analysis, Isotope Labeling-methods, Nuclear Magnetic Resonance, Biomolecular Here we describe a protocol for the labeling of proteins that facilitates their study using a technique that is sensitive to millisecond conformational exchange restrict to techniques of labeling with isotopes (stable or radioactive): do not use for radioisotope tracer studies of "labeled" cells, microorganisms or chemicals Pris: 1862 kr. (firebaseapp.com)
12. C. This gives a Amino-acid selective isotope labeling of proteins offers numerous advantages in mechanistic studies by revealing structural and functional information unattainable from a crystallographic approach. (firebaseapp.com)
However, efficient labeling of proteins with selected amino acids necessitates auxotrophic hosts, which are often not available. (firebaseapp.com)
Researchers then analysed these samples to measure the incorporation of the tracer into specific muscle protein fractions and individual proteins. (uticahistoricalsociety.org)
A new study, published in eLife, uses advanced tissue analysis technology to show how the incorporation of new proteins changes in bone and cartilage with age. (ox.ac.uk)
We investigated the entire set of proteins modulated by BAG3 silencing in the human anaplastic thyroid 8505C cancer cells by using the Stable-Isotope Labeling by Amino acids in Cell culture strategy combined with mass spectrometry analysis. (oncotarget.com)

Their tiny size, rapid growth by clonal propagation, and facile uptake of labeled compounds from the media were attractive features that made them a well-known model for plant biology from 1950 to 1990. (osti.gov)
Our data point toward a fast and exclusive uptake of labeled necromass by a few specialists followed by a concerted action of groundwater microorganisms, including autotrophs presumably fueled by released, reduced nitrogen and sulfur compounds generated during necromass degradation. (ufz.de)

Cells grown on yeast extract in the presence of DL-[3,3-2H2]serine contained 40.1 mol% of the protein-bound serine and 5.0 mol% of the protein-bound cysteine derived from the labeled serine. (asm.org)
Measures the incorporation of stable isotope-labeled serine (substrate) into sphinganine, sphinganine-1-phosphate and dihydroceramides. (sidra.org)

Different 13C-labelled substrates (glucose, acetate and pyruvate) at low concentrations (100 µM) were used over a 7-day incubation to follow and identify carbon flow into different members of the community. (cardiff.ac.uk)

Labeling occurs by site-specific incorporation of stable isotope labeled tags at cysteine residues or the general labeling of amines and carboxyl groups in protein samples. (firebaseapp.com)

The combination of high-resolution LC-MS-based untargeted metabolomics with stable isotope tracing provides a global overview of the cellular fate of precursor metabolites. (acs.org)
New metabolomics applications of ultra-high resolution and accuracy mass spectrometry can provide thousands of detectable isotopologues, with the number of potentially detectable isotopologues increasing exponentially with the number of stable isotopes used in newer isotope tracing methods like stable isotope-resolved metabolomics (SIRM) experiments. (mdpi.com)

For example, the medium can contain arginine labeled with six carbon-13 atoms (13C) instead of the normal carbon-12 (12C). (wikipedia.org)
Using different isotopes of carbon, hydrogen, nitrogen, and oxygen, the researchers worked out 39 distinct isotopologues for lysine spanning a mass range of roughly 39 mDa and separated by 1 mDa each. (genomeweb.com)
Labeling of Trypanosoma brucei cell cultures with 50% uniformly 13C-labeled glucose demonstrated incorporation of glucose-derived carbon into 187 of 588 putatively identified metabolites in diverse pathways including carbohydrate, nucleotide, lipid, and amino acid metabolism. (acs.org)
However, in addition to carbon, other elements such as oxygen in the nature also have natural stable isotopes (e.g., 17O, 18O). (biomed.news)
We have applied the recently developed method of protein-based stable isotope probing (protein-SIP) to track carbon flow in a mixed culture of acidophilic bacteria. (jiomics.com)
Ecosystem 13 CO 2 -pulse-labeling showed that drought enhanced the mean residence times of freshly assimilated carbon- indicating down-regulation of carbon cycling velocity and delayed transport form leaves to trunk and roots. (copernicus.org)
I typically use a combination of controlled lab-based experiments in parallel with assessments of soils from field-experiments and natural gradients, and employ a series of radio- and stable-isotope based method in order to resolve how bacterial ( 3 H-leucine and 3 H-thymidine incorporation) and fungal ( 14 C-acetate-in-ergosterol) growth drive the processes of carbon and gross nitrogen ( 15 N pool-dilution) mineralization in soils. (lu.se)

Because of reluctance to use radioisotopes for studies of iron absorption in children, we have explored the feasibility of using the least abundant stable isotope of iron, 58Fe (natural abundance, 0.322 weight %) in a study of nonheme iron absorption. (unboundmedicine.com)

SIP using labeled 13 C compounds has shed light onto how the soil microbiome metabolizes certain molecules of interest such as cellulose 17 . (nature.com)

In their study released this week, Coon and his team built on this work by incorporating a number of different isotope combinations into lysine molecules, which they then used to metabolically label proteomics samples. (genomeweb.com)
Real-time measurements on polymerization reactions provide both mechanistic and kinetic information without the need for stable isotope labeling of the molecules of interest. (bridge12.com)

2010-04-07 This putative identification is corroborated by mass spectrometric fragmentation, isotope labeling, and a gene silencing experiment. (firebaseapp.com)

This methodology enables detection of putative metabolites from biological samples and simultaneous quantification of the pattern and extent of isotope labeling. (acs.org)

We traced ecosystem scale interactions through a whole-ecosystem labelling approach in the Biosphere 2 Tropical Rainforest, the B2 Water, Atmosphere, and Life Dynamics (B2WALD) experiment. (copernicus.org)
Further, a laboratory experiment will be conducted, where under controlled conditions only soil moisture is regulated to drought and control conditions, respectively and via a continuous 13CO2 labeling C cycling in the plant-soil system is determined. (uzh.ch)

Some newer approaches have coupled SIP to single cell analysis using Raman microspectroscopy or NanoSIMS to track metabolically active or newly formed cells 23 by labeling them with H 2 18 O 24 , but these methods currently have relatively low throughput and provide limited phylogenetic resolution. (nature.com)
Detailed in a paper published this week in Nature Methods , the technique, called NeuCode, uses differences in the nuclear binding energy of different isotopes to label amino acids. (genomeweb.com)
M. Takeda, M. Kainosho, in Comprehensive Isotope Labeling of Biomolecules - Labeling Methods. (firebaseapp.com)

We will measure 13CO2 fluxes in the field and combine these data with the incorporation of the 13C tracer into different SOM fractions. (tu-braunschweig.de)

Marine sediment slurries enriched for anaerobic, sulfate-reducing prokaryotic communities utilizing glucose and acetate were used to provide the first comparison between stable-isotope probing (SIP) of phospholipid fatty acids (PLFA) and DNA (16S rRNA and dsrA genes) biomarkers. (cardiff.ac.uk)
A broad range of PLFA were rapidly labelled (within 12 h) in the 13C-glucose slurry but this changed with time, suggesting the presence of an active glucose-utilizing population and later development of another population able to utilize glucose metabolites. (cardiff.ac.uk)
The identity of the major glucose-utilizers was unclear as 13C-enriched PLFA were common (16:0, 16:1, 18:1ω7, highest incorporation) and there was little difference between 12C- and 13C-DNA 16S rRNA gene denaturing gradient gel electrophoresis (DGGE) profiles. (cardiff.ac.uk)
Seemingly glucose, a readily utilizable substrate, resulted in widespread incorporation consistent with the higher extent of 13C-incorporation (∼10 times) into PLFA compared with 13C-acetate or 13C-pyruvate. (cardiff.ac.uk)
incorporation of acetate and glucose (or glucose metabolites) under anaerobic, sulfate-reducing conditions. (cardiff.ac.uk)

The bilayers are labelled with a rhodamine phospholipid (red). (lu.se)

The biosynthesis of the sulfonolipid 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid (capnine) was studied by measuring the incorporation of possible precursors into the lipid by cells grown in the presence of precursors which were labeled with stable isotopes. (asm.org)
The heterotroph Acidiphilium cryptum was grown in the presence of 13 C-labeled galactose, together with the iron- and sulfur-oxidizing autotroph Acidithiobacillus ferrooxidans . (jiomics.com)

Additionally a metabolism study with [1′-C-13, 3′, 3′-H-2(2)]-hyoscyamine has established that the alkaloid is metabolically stable at C-3′ with no evidence for a reversible in vivo oxidation process to the corresponding aldehyde. (sinapse.ac.uk)

Strong effects of drought were described for the regulation of lipid formation in plants including fatty acids as part of cell membranes and alkanes as part of the wax layer, whereas so far no information is available, if lipid incorporation into soils and lipid turnover therein is also influenced by drought. (uzh.ch)
However, as influences of drought on lignin and lipid cycling in the plant-soil system remain largely unknown, but they are part of intermediate stable C pool in soil, it should be known, whether drought might improve their mineralisation or storage in soils. (uzh.ch)
In addition to the bulk C, lipids including fatty acids, alkanes and alcohols and lignin monomers will be monitored for the whole sample set to determine the regulation of lipid and lignin formation in plants under drought and especially to investigate the incorporation and mineralisation of bulk C and at a molecular level for lipids and lignin during the drought. (uzh.ch)

The mass isotope ratio, 58Fe/57Fe (MIR58/57), was measured in blood by inductively coupled plasma mass spectroscopy before and 14 days after (i.e. study day 15) consuming the three lunches. (unboundmedicine.com)

This technique allows non-destructive, label-free spectral imaging and analysis of cells, tissues, and nanoparticles [ 5 - 7 ]. (medsci.org)
Using stable isotope labeling and siRNA-mediated gene silencing, we showed that A549 lung cancer cells produce NAA and its downstream … 2019-06-26 15 N-labeling. (firebaseapp.com)
KP2156 forms very stable albumin adducts in the bloodstream resulting in a superior pharmacological profile, such as distinctly prolonged terminal excretion half-life and enhanced effective platinum dose (measured by ICP-MS). The albumin-bound drug is accumulating in the malignant tissue, where it enters the cancer cells clathrin- and caveolin-dependent endocytosis, and is activated by reduction to release oxaliplatin. (microbial-ecology.de)

Active microorganisms have been identified previously using stable isotope probing (SIP) or bromodeoxyuridine (BrdU) labeling. (nature.com)

We followed the uptake of 13 C-labeled necromass by the bacterial and eukaryotic groundwater community quantitatively and over time using a complementary protein-stable and DNA-stable isotope probing approach. (ufz.de)
Limited changes in total PLFA and bacterial 16S rRNA gene DGGE profiles over 7 days suggested the presence of a stable bacterial community. (cardiff.ac.uk)

University of Wisconsin-Madison researchers have developed a new labeling approach that could enable significantly higher multiplexing in mass spec proteomics experiments. (genomeweb.com)

We analysed total ecosystem exchange, soil and leaf fluxes of H 2 O, CO 2 and BVOCs, and their stable isotopes over five months. (copernicus.org)
To trace changes in soil-plant-atmosphere interactions we labelled the ecosystem with a 13 CO 2 -isotope. (copernicus.org)

We will study above- and belowground litter decomposition and SOM stabilization by applying 13C-labelled litter (shoots and roots) of an annual herbaceous plant in the field along the aridity gradient. (tu-braunschweig.de)

The increased multiplexing capability of NeuCode amino acids is from the use of mass defects from extra neutrons in the stable isotopes. (wikipedia.org)
Cambridge Isotope Laboratories offers a complete listing of Stable Isotope Labeled Mass Spec for all your research needs. (firebaseapp.com)
The method was established based on the stable isotope-dilution liquid chromatography-tandem mass spectroscopy (ID-LC-MS/MS) technique. (biomed.news)
In the present study, diverse cell biological and analytical tools such as laser ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS), isotope labeling, and nano-scale secondary ion mass spectrometry (NanoSIMS) were employed to better understand the distribution and activation process of KP2156 (in comparison to free oxaliplatin and a non-albumin-binding succinimide analogue). (microbial-ecology.de)

Using an NMR chemical shift prediction approach, several minor products that showed 13C incorporation were identified as organic acids, amino acids, and various alcohols. (tno.nl)

In this paper, we proposed a method for construction of correction matrix based on Python language for correcting the measurement errors due to natural isotope distribution. (biomed.news)
We validate this new algorithm against a previous single isotope correction algorithm in a two-step cross-validation. (mdpi.com)

Nuclear binding energy is the energy needed to break a nucleus up into its component nucleons, and because every isotope has a unique nuclear binding energy, these differences can be used to distinguish between them. (genomeweb.com)

However, incorporation of different isotopes significantly increases sample complexity, which can reduce peptide IDs if taken too far. (genomeweb.com)
Tonia Vincent, Professor of Musculoskeletal Biology said: "Although new protein incorporation changes significantly in all tissues after skeletal maturity, it displays distinct temporal and molecular tissue signatures. (ox.ac.uk)

As a new type of probe, the heavy isotope of water has been widely used in the study of cell development, metabolism, tissue homeostasis, aging, and tumor heterogeneity. (medsci.org)

Alternatively, uniform labeling with 13C or 15N can be used. (wikipedia.org)

Matrix effects were evaluated by spiking the samples with isotope-labeled standards of 40 polar contaminants. (firebaseapp.com)

One method for measuring MPS is by using stable isotopes. (uticahistoricalsociety.org)
The enzymatic dideoxy chain termination sequencing method of Sanger [1] relies on the linear amplification of a single-stranded template DNA using a 5'-fluorescently labeled primer and a modified Taq polymerase. (jenabioscience.com)

Incorporation of 58Fe into erythrocytes was greater from the lunch with beef patty than from the lunch with beef-soy patty (geometric mean values 2.02 and 1.05% of the dose, p less than 0.03). (unboundmedicine.com)
Iron bioavailability was assessed by measuring iron incorporation into erythrocytes 2 weeks after consumption. (micronutrientforum.org)

Owing to the complexity of the problems involved, the suitability of pesticides for a particular purpose and the content of the labelling instructions must be decided at the national or provincial level. (who.int)

The study found that comparing young adult with older adult mice, new protein incorporation was reduced in all tissues and this was particularly marked in cartilage and bone. (ox.ac.uk)

Anatomy17

Organisms32

Diseases1

Chemicals and Drugs86

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Psychiatry and Psychology7

Phenomena and Processes55

Disciplines and Occupations9

Anthropology, Education, Sociology and Social Phenomena6

Technology, Industry, Agriculture6

Humanities1

Information Science5

Health Care19

Geographicals3

Analyses of the isotope2

Isotopically labeled2

Metabolic10

SILAC10

Heavy isotopes2

Peptides10

Isotopic3

Quantitative3

Synthesis4

Proteins6

Uptake of labeled2

Serine2

Substrates1

Cysteine1

Metabolomics2

Carbon7

Abundance1

Compounds1

Molecules2

Fragmentation1

Quantification1

Experiment2

Methods3

Tracer1

Glucose5

Phospholipid1

Grown in the presence2

Metabolically1

Lipid3

Ratio1

Cells3

Microorganisms1

Bacterial2

Researchers1

Soil2

Stabilization1

Mass4

Approach1

Correction2

Nuclear1

Significantly2

Widely1

Uniform1

Samples1

Method2

Erythrocytes2

Complexity1

Tissues1