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  • moiety
  • Nucleic acids may be labeled by complexing the alkylating moiety of a labeling reagent into a single-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. (google.com)
  • More particularly, this invention is directed to a process for preparing labeled single-stranded nucleic acids by use of an alkylating agent containing a label moiety, where the word label is intended to include moieties which may be detected both directly and indirectly. (google.com)
  • enzymes
  • Mammalian Gpx1, GPx2 (this protein), Gpx3, and Gpx4 have been shown to be selenium-containing enzymes, whereas Gpx6 is a selenoprotein in humans with cysteine-containing homologues in rodents. (wikipedia.org)
  • uracil
  • Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. (wikipedia.org)
  • genetic material
  • In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane(s). (wikipedia.org)
  • gene
  • Here we report the isolation of the gene MHY1 encoding a protein, Mhy1p, involved in the yeast-to-hypha transition in Y. lipolytica . (pubmedcentralcanada.ca)
  • they can either replace the host gene with one that codes for a different protein, or introduce an additional gene. (wikipedia.org)
  • Histone H2A.Z is a protein that in humans is encoded by the H2AFZ gene. (wikipedia.org)
  • Unlike replication, where a complete copy of DNA is made, transcription copies only the gene that is to be expressed as a protein. (wikipedia.org)
  • polymerases
  • Deazaadenosine was used for this second-generation conjugate as it is a better mimic of a natural DNA base than is the first-generation substituted benzene C-nucleoside which facilitates its recognition and incorporation by promiscuous DNA polymerases. (docme.ru)
  • Incorporation of reporter-labeled nucleotides by DNA polymerases. (semanticscholar.org)
  • sodium
  • A total of 72 root dentine slices were divided into six groups, according to the final irrigants used: Group 1: 17% ethylenediamine tetra-acetic acid (EDTA) + 2.5% NaOCl, Group 2: 7% maleic acid (MA) + 2.5% sodium hypochloride (NaOCl), Group 3: 1.3% NaOCl + mixture of tetracycline, acid and detergent (MTAD), Group 4: Smear Clear + 2.5% NaOCl, Group 5: 5% NaOCl, Group 6: saline. (jove.com)
  • mixture
  • A small volume of the hybridization mixture is placed onto a coverslip and then placed gently onto the microscope slide which contains the fixed cells. (wikipedia.org)
  • Encodes
  • MHY1 encodes a C 2 H 2 -type zinc finger protein, Mhy1p, which can bind putative cis -acting DNA stress response elements, suggesting that Mhy1p may act as a transcription factor. (pubmedcentralcanada.ca)
  • The human genome encodes approximately 25,000 genes but there are significantly more proteins produced. (wikipedia.org)
  • Hemagglutinin
  • Hemagglutinin (HA) and neuraminidase (NA) are two surface proteins of influenza viruses which are known to play important roles in the viral life cycle and the induction of protective immune responses 1,2 . (jove.com)
  • viral
  • Although here we focus on influenza virus surface glycoproteins, this method can also be used to produce other viral and cellular surface proteins. (jove.com)
  • MDCK cells (10 4 or 5 x 10 3 per well) were cultured in 96- or 384-well plates, infected with PR8 and viral proteins were detected using anti-M2 followed by an IR dye-conjugated secondary antibody. (jove.com)
  • interactions
  • Aside from their benefit to studies of the dynamics of protein-ligand interactions, these antibodies can be envisioned in a number of practical applications. (docme.ru)
  • strains
  • However the protocol can be easily adapted for HA and NA proteins expressed by any other influenza A and B virus strains. (jove.com)
  • production
  • Other experiments show that a single miRNA species may repress the production of hundreds of proteins, but that this repression often is relatively mild (much less than 2-fold). (wikipedia.org)
  • While DNA serves as template for production of ribonucleic acid (RNA), RNA is usually responsible for making protein. (wikipedia.org)
  • The first company to focus on genetic engineering, Genentech, was founded in 1976 and started the production of human proteins. (wikipedia.org)
  • either
  • Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pentose sugar, either ribose or deoxyribose, and one of four nucleobases. (wikipedia.org)
  • This non-native segment of DNA may either retain the ability to produce RNA or protein in the transgenic organism or alter the normal function of the transgenic organism's genetic code. (wikipedia.org)
  • provide
  • Although they do not provide covalent labeling, the Zenon Antibody Labeling Kits are listed in Active esters and kits for labeling proteins and nucleic acids-Table 1.2 , as well as in Zenon Antibody Labeling Kits-Table 7.7 . (thermofisher.com)