• This gene encodes a member of a family of E3 small ubiquitin-related modifier (SUMO) ligases that mediates the attachment of a SUMO protein to proteins involved in nuclear transport, transcription, chromosome segregation and DNA repair. (nih.gov)
  • Chromobox4 (CBX4), also named hPC2, is a small ubiquitin-related modifier (SUMO) E3 ligase. (scienceopen.com)
  • o-Succinylbenzoate-CoA ligase (EC 6.2.1.26), encoded from the menE gene in Escherichia coli, catalyzes the fifth reaction in the synthesis of menaquinone (vitamin K2). (wikipedia.org)
  • Organisms that contain a pathway similar to this include Arabidopsis thaliana (gene AAE14), Mycobacterium phlei, and Synechocystis sp. (wikipedia.org)
  • Naturally occurring mutations in this gene, that abolish the SUMO ligase activity, are associated with primordial dwarfism and extreme insulin resistance. (nih.gov)
  • Succinate-CoA ligase deficiency results from mutations in the SUCLA2 or SUCLG1 gene. (medlineplus.gov)
  • Mutations in either the SUCLA2 or SUCLG1 gene disrupt the normal function of succinate-CoA ligase. (medlineplus.gov)
  • E3 ubiquitin-protein ligase Mdm2 is a protein encoded by the MDM2 gene. (researchandmarkets.com)
  • An E. coli strain that carries the cloned truncated T4 RNA Ligase 2 gene. (neb.com)
  • UFL1 (UFM1 Specific Ligase 1) is a Protein Coding gene. (thermofisher.com)
  • Gene Ontology (GO) annotations related to this gene include ligase activity and UFM1 transferase activity. (thermofisher.com)
  • Three gene expression datasets including GSE116959, GSE74706, and GSE85841 downloaded from the Gene Expression Omnibus (GEO) database were used in this study. (hindawi.com)
  • Analysis of an 11.7 kb DNA fragment of chromosome XI reveals a new tRNA gene and four new open reading frames including a leucine zipper protein and a homologue to the yeast mitochondrial regulator ABF2. (wikigenes.org)
  • The Neisseria gonorrhoeae assay uses LCR TM (ligase chain reaction) amplification technology in the LCx Probe System for the direct, qualitative detection of a specific target nucleic acid sequence in the Opa gene of Neisseria gonorrhoeae . (cdc.gov)
  • The peripheral protein quality control (periQC) system eliminates the conformationally defective cystic fibrosis transmembrane conductance regulator (CFTR), including ∆F508-CFTR, from the plasma membrane (PM) and limits the efficacy of pharmacological therapy for cystic fibrosis (CF). The ubiquitin (Ub) ligase RFFL is responsible for the chaperone-independent ubiquitination and lysosomal degradation of CFTR in the periQC. (nih.gov)
  • Numerous E3 ubiquitin ligases, which facilitate the ubiquitination of specific substrates, have been shown to control G1/S. In this chapter, we will discuss components in the ubiquitin proteasome system that are implicated in G1/S control, how these enzymes are interconnected, gaps in our current knowledge, and the potential role of these pathways in the cancer cycle and disease proliferation. (intechopen.com)
  • B ) Enzymatic cascade of ubiquitination, including the ATP-dependent activation of ubiquitin ( E1 ), ubiquitin conjugation ( E2 ), and ultimate ubiquitin transfer to target substrate ( E3 ). (elifesciences.org)
  • CHIP is an E3 ligase, recognizing Hsp70-bound substrates via the TPR binding domain and catalyzing their ubiquitination via the U-box domain (hexagon). (elifesciences.org)
  • Moreover, we observed that PUS10 is ubiquitinated by E3 ubiquitin ligase CRL4DCAF1 complex and the increase of PUS10 in aged HSPC is due to aging-declined CRL4DCAF1- mediated ubiquitination degradation signaling. (haematologica.org)
  • Ces molécules contrôlent ce processus en favorisant l'internalisation du récepteur de l'antigène des cellules B et la sélection vers les lysosomes pour la dégradation par ubiquitination de l'Ig, un composant du complexe BCR. (umontreal.ca)
  • Bsd2 binds the ubiquitin ligase Rsp5 and mediates the ubiquitination of transmembrane proteins. (wikigenes.org)
  • RNF2 (E3 ubiquitin-protein ligase RING2) is a ligase that mediates monoubiquitination of Lys-119 of histone H2A (H2AK119Ub). (fishersci.com)
  • Her main research interests have been the study of Mur ligases from different bacterial species and the search for inhibitors of these enzymes. (degruyter.com)
  • The synthesis of the peptide stem of bacterial peptidoglycan involves four enzymes, the Mur ligases (MurC, D, E and F). Among them, MurD is responsible for the ATP-dependent addition of d -glutamic acid to UDP-MurNAc- l -Ala, a reaction which involves acyl-phosphate and tetrahedral intermediates. (degruyter.com)
  • In her new project, UPSmeetMet, Schulman and her team will use state-of-the art methods, and develop new technologies, to understand how molecular machines, the E3 ligases, specifically recognize and control metabolic enzymes that cells need to destroy. (izb-online.de)
  • role of ubiquitin ligases in autophagy and of ubiquitin-modifying enzymes in the regulation of the immune response. (brookes.ac.uk)
  • Cells depend on their DNA for coding information to make various classes of proteins that include enzymes, certain hormones, transport proteins, and structural proteins that support life. (cdc.gov)
  • Recently, we reported that the ubiquitin E3 ligase ITCH negatively regulates LATS1, thereby increasing YAP activity, which leads to increased cell proliferation and decreased apoptosis. (oncotarget.com)
  • p53 E3 ubiquitin protein ligase homolog (Mdm2) is an important negative regulator of p53. (molvis.org)
  • In the model organism Dictyostelium discoideum , loss of the CLN5 homolog, cln5 , impacts various cellular and developmental processes including cell proliferation, cytokinesis, aggregation, cell adhesion, and terminal differentiation. (frontiersin.org)
  • DNA DSB repair E3 ligase genes are involved in tumor suppressive or oncogenic functions, such as RNF168 and FBXW7, by assisting the functionality of these genomic alterations. (bmbreports.org)
  • The SUCLA2 and SUCLG1 genes each provide instructions for making one part (subunit) of an enzyme called succinate-CoA ligase. (medlineplus.gov)
  • Cell debris was removed by centrifugation (13,000 rpm for 15 minutes) and DNA extract stored at -70°C. Genetic identification of enterococci to species level and detection of vanA, vanB, vanC1, or vanC2/C3 and vanD ligase genes were performed by using a multiplex polymerase chain reaction amplification ( 7 ). (cdc.gov)
  • This amplification detects both structural genes encoding D-alanine-D-alanine ligases and glycopeptide resistance genes. (cdc.gov)
  • This complex class is required to maintain the transcriptionally repressive state of many genes including Hox genes throughout development. (fishersci.com)
  • We list the DNA repair genes and related E3 ligases. (bmbreports.org)
  • The 11 genes with a high frequency of mutation differed between cancers, and mutations in many DNA DSB repair E3 ligase genes were related to a higher total mutation burden. (bmbreports.org)
  • Effects of Nephrolithiasis on Serum DNase (Deoxyribonuclease I and II) Activity and E3 SUMO-Protein Ligase NSE2 (NSMCE2) in Malaysian Individuals. (nih.gov)
  • Previous studies have identified that UBE3A exhibits abnormal expression in numerous tumor cells, including prostate, cervical and breast cancer ( 6 , 7 ). (spandidos-publications.com)
  • The reaction in vitamin K synthesis that includes MenE is as follows: ATP + 2-succinylbenzoate + CoA = AMP + diphosphate + 4-(2-carboxyphenyl)-4-oxobutanoyl-CoA The substrates of this reaction are ATP, CoA, and 2-succinylbenzoate. (wikipedia.org)
  • We structurally resolve the interface with PRMT5 and show via genetic perturbation that it is required for methylation of adaptor-recruited substrates including the spliceosome, histones, and ribosomal complexes. (rcsb.org)
  • E3 Ubiquitin Protein Ligase Mdm2 (Double Minute 2 Protein or Hdm2 or Oncoprotein Mdm2 or p53 Binding Protein Mdm2 or MDM2 or EC 6.3.2. (researchandmarkets.com)
  • The latest report E3 Ubiquitin Protein Ligase Mdm2 - Pipeline Review, H1 2020, outlays comprehensive information on the E3 Ubiquitin Protein Ligase Mdm2 (Double Minute 2 Protein or Hdm2 or Oncoprotein Mdm2 or p53 Binding Protein Mdm2 or MDM2 or EC 6.3.2. (researchandmarkets.com)
  • The report provides a snapshot of the global therapeutic landscape for E3 Ubiquitin Protein Ligase Mdm2 (Double Minute 2 Protein or Hdm2 or Oncoprotein Mdm2 or p53 Binding Protein Mdm2 or MDM2 or EC 6.3.2. (researchandmarkets.com)
  • The report reviews latest news and deals related to E3 Ubiquitin Protein Ligase Mdm2 (Double Minute 2 Protein or Hdm2 or Oncoprotein Mdm2 or p53 Binding Protein Mdm2 or MDM2 or EC 6.3.2. (researchandmarkets.com)
  • Identify and understand the targeted therapy areas and indications for E3 Ubiquitin Protein Ligase Mdm2 (Double Minute 2 Protein or Hdm2 or Oncoprotein Mdm2 or p53 Binding Protein Mdm2 or MDM2 or EC 6.3.2. (researchandmarkets.com)
  • The Chlamydia trachomatis assay uses LCR TM (ligase chain reaction) amplification technology in the LCx Probe System for the direct, qualitative detection of plasmid DNA of Chlamydia trachomatis . (cdc.gov)
  • p53 participates in relevant aspects of cell biology, including apoptosis and cell cycle control and must be strictly regulated to maintain normal tissue homeostasis. (molvis.org)
  • Here, we report that the Ub ligase RNF34 participates in the CFTR periQC in parallel to RFFL. (nih.gov)
  • 13-15 Ψ plays an important role in various aspects of RNA biology, and, therefore, participates in many biological process, including translational control, 16 , 17 RNA folding, 18-22 protein translation, 23-26 and clinical diseases. (haematologica.org)
  • Succinate-CoA ligase deficiency is an inherited disorder that affects the early development of the brain and other body systems. (medlineplus.gov)
  • Most children with succinate-CoA ligase deficiency also experience a failure to thrive, which means that they gain weight and grow more slowly than expected. (medlineplus.gov)
  • Succinate-CoA ligase deficiency causes breathing difficulties that often lead to recurrent infections of the respiratory tract. (medlineplus.gov)
  • These infections can be life-threatening, and most people with succinate-CoA ligase deficiency live only into childhood or adolescence. (medlineplus.gov)
  • A few individuals with succinate-CoA ligase deficiency have had an even more severe form of the disorder known as fatal infantile lactic acidosis. (medlineplus.gov)
  • Although the exact prevalence of succinate-CoA ligase deficiency is unknown, it appears to be very rare. (medlineplus.gov)
  • These problems lead to hypotonia, muscle weakness, and the other characteristic features of succinate-CoA ligase deficiency. (medlineplus.gov)
  • Beyond this basic grouping, SCID may be categorized according to phenotypic lymphocyte profiles that include both B-cell status (B + or B - ) and NK-cell status (NK + or NK - ) in addition to T-cell status (T - , because there is always a T-cell deficiency in SCID). (medscape.com)
  • Dysfunction of mitochondria and UPS increases with age and correlates with many age-related diseases including cancer and neurodegeneration. (hindawi.com)
  • Failure of the PN is associated with broad range of diseases including cancer, neurodegeneration, and immunological and metabolic disorders [ 1 ]. (hindawi.com)
  • The reason for the difference in pathways is due to the varying functions of Vitamin K. Eukaryotic bacteria use vitamin K II while other organisms use vitamin K I. Other pathways that include o-succinylbenzoate CoA ligase include 1, 4-diydroxy-2-naphthoate biosynthesis II (i.e. in Arabidopsis thaliana), biosynthesis of secondary metabolites, metabolic pathways, ubiquinone, and other terpenoid-quinone biosynthesis. (wikipedia.org)
  • We are open to receive contributions to the Research Topic "E 3 Ubiquitin Ligases: From Structure to Physiology to Therapeutics, Volume II ", which is part of the E3 Ubiquitin Ligases series. (brookes.ac.uk)
  • Silencing Txnip prompted TLR2-mediated inducible nitric oxide synthase (iNOS)/NO, TNF-α, and IL-6 production whereas the blockage of Txnip degradation by pharmacologically inhibiting the HECT E3 ubiquitin ligase with heclin and AMP-dependent protein kinase with dorsomorphin effectively reduced such effects. (frontiersin.org)
  • DNA ligase (polydeoxyribonucleotide synthase) is the enzyme that joins two DNA fragments by catalysing the formation of an internucleotide ester bond between phosphate and deoxyribose. (embl.de)
  • We are a community of more than 103,000 authors and editors from 3,291 institutions spanning 160 countries, including Nobel Prize winners and some of the world's most-cited researchers. (intechopen.com)
  • Inhibitors of this enzyme include diethylprocarbonate, Fe(2+), Hg(2+), and Mg(2+) (above 1mM). (wikipedia.org)
  • The development of chemical tools for the ubiquitin system including the discovery of new inhibitors for E3 ligases and the use of these ligands for PROTAC development. (uni-frankfurt.de)
  • Structural studies and the development of chemical tools for the human protein kinase family including covalent and allosteric inhibitors. (uni-frankfurt.de)
  • Unlike the full length ligase, T4 Rnl2 truncated is unable to adenylate the 5´ end of the substrate, and as a result it cannot ligate the phosphorylated 5´ end of RNA or DNA to the 3´ end of RNA (1-3). (neb.com)
  • C ) Schematic for engineering an E3 ubiquitin ligase and potential outcomes on an ion channel substrate. (elifesciences.org)
  • The Mouse ON-TARGET plus RTF Ubiquitin Conjugation Subset 2 siRNA Library targets F-box and SOCS box E3 ubiquitin ligases, which are substrate recognition elements for Cul-1 and Cul-2 containing ubiquitin ligases. (horizondiscovery.com)
  • We demonstrate that PRMT5 uses modular adaptor proteins containing a common binding motif for substrate recruitment, comparable with other enzyme classes such as kinases and E3 ligases. (rcsb.org)
  • lister encodes a RING finger protein, LISTERIN, which functions as an E3 ubiquitin ligase in vitro. (nih.gov)
  • In vitro studies showed the mezigdomide-induced degradation of the two factors enhanced cytotoxic effects in myeloma cells, including cells resistant to IMiDs lenalidomide (Revlimid) and pomalidomide (Pomalyst) and with cereblon down-regulation. (medpagetoday.com)
  • Here we focus on WWP2, an E3 ubiquitin ligase that acts as a positive genetic regulator of human and murine cardiac fibrosis, and show that myeloid specific deletion of WWP2 reduces cardiac fibrosis in hypertension-induced NICM. (nature.com)
  • The overexpression of miRNA‑375 significantly suppressed the levels of protein expression of ubiquitin‑protein ligase E3A (UBE3A) and Insulin‑like growth factor‑1 receptor (IGF‑1R) in HPV‑18(+) cervical cancer cells. (spandidos-publications.com)
  • We recently described the structure of M. musculus RNF213 (PDB: 6TAX) and characterized its enzymatic activity, revealing it as a large monomeric protein with a functional dynein-like AAA (ATPases Associated with diverse cellular Activities) core that exists alongside a novel E3 ligase module ( 16 ). (biorxiv.org)
  • The ligase activity is enhanced by BMI1/PCGF4. (fishersci.com)
  • An extract of the coralline demosponge Astrosclera willeyana inhibited the ubiquitin ligase activity of the immunomodulatory protein Cbl-b. (cancer.gov)
  • Spatially regulated ubiquitin ligation by an ER/nuclear membrane ligase. (wikigenes.org)
  • We integrate structural biology methods, including NMR (nuclear magnetic resonance) spectroscopy and protein X-ray crystallography, to study the protein-protein interactions underpinning cell division regulation. (brookes.ac.uk)
  • Thioredoxin-interacting protein (Txnip), a vitamin D 3 -upregulated protein in 1α,25-dihydroxyvitamin D 3 (1,25[OH] 2 D 3 )-treated HL-60 cells ( 7 ), acts as an endogenous inhibitor of the antioxidant thioredoxin (Trx), which is involved in a wide variety of cellular processes including the response to oxidative stress, cancer development, metabolic diseases, and inflammatory processes ( 8 - 13 ). (frontiersin.org)
  • The molecular weight of o-succinylbenzoate CoA ligase is 185000 Da or 185 kDa. (wikipedia.org)
  • Over the past few decades - including through support from a current ERC Advanced Grant - Schulman and her team have deciphered the complex "molecular machines", called E3 ligases, that ensure that the UPS destroys only those proteins whose functions should be terminated. (izb-online.de)
  • To understand the molecular role of polarity proteins in plant development, it is important to characterize their interactome, including their protein-protein interaction partners. (oeaw.ac.at)
  • The catalytic region comprises six conserved sequence motifs (I, III, IIIa, IV, V-VI), motif I includes the lysine residue that is adenylated in the first step of the ligation reaction. (embl.de)
  • Those guidelines highlighted the prevalence and morbidity of chlamydial infections and stressed the need to include antibiotics effective against chlamydia when treating patients for urethritis, mucopurulent cervicitis, and pelvic inflammatory disease. (cdc.gov)
  • The prevalence of CT among our sample · behavioural variables including age at was 11.6% (64/550). (who.int)
  • It is a non-communicable disease that includes a range of physiological disorders which are attributed to abnormal renal function and its progressive decline infiltration rate (the glomerular filtration rate). (news-medical.net)
  • For instance, mice are able to reconstitute most lymphomas in monkeys and humans woodchuck hepatitis virus induces major components of the human provides strong support for a direct hepatocellular carcinoma (HCC) haematolymphoid system including oncogenic role of EBV in vivo. (who.int)
  • The scheme funded some 20 studentships in the biological sciences in the Oxford area including the Pirbright Institute, the Harwell Campus Oxford (Diamond Light Source, ISIS Neutron and Muon Source, STFC Central Laser Facility and the Research Complex at Harwell). (brookes.ac.uk)
  • RNF213/ALO17/mysterin (Ring Finger Protein 213) is the largest known human single-chain E3 ubiquitin ligase, first described as the major susceptibility factor for Moyamoya disease (MMD) ( 1 ), a disorder which causes aberrant vasculature development commonly leading to stroke and other complications ( 2 ). (biorxiv.org)
  • The ubiquitin ligase E6-AP (E6-associated protein) represents a prime example for the notion that deregulated modification of proteins with ubiquitin contributes to the development of human disease: loss of E6-AP function by mutation is responsible for the development of AS (Angelman syndrome), a neurological disorder, and unscheduled activation of E6-AP by complex formation with the E6 oncoprotein of HPVs (human papillomaviruses) contributes to cervical carcinogenesis. (portlandpress.com)
  • Connections to non-vascular diseases were also reported, including diabetes ( 7 ), cancer ( 8 ) and both viral ( 9 ) and bacterial ( 10 ) infection. (biorxiv.org)
  • Eukaryotic, archaebacterial, viral and some eubacterial DNA ligases are ATP-dependent. (embl.de)
  • MenE belongs to the ligase enzyme family, or class 6. (wikipedia.org)
  • E3 ligases consist of a large family of more than 600 proteins ( 3 ), and a few E3 ligases have been classified based on their conserved structural domains (e.g. (bmbreports.org)
  • Well-known members of this E3 ligase family include bTRCP, SKP2, and VHL. (horizondiscovery.com)
  • Ubiquitin ligases refers to a family of proteins that are widely distributed in animals and plants and act as key regulators of cell metabolism and cell signaling. (brookes.ac.uk)
  • For this reason, the infect humans, this virus can infect cordance between humans and ex- question about tumour site concor- several other species - including perimental animals. (who.int)
  • As part of the Structural Genomics Consortium ( SGC ) our laboratory is committed to "Open science and innovation" und unencumbered release of research reagents including chemical probes and chemogenomic compound collections . (uni-frankfurt.de)
  • DNA damage response-related E3 ligases, such as RNF168, FBXW7, and HERC2, were generated with more than 10% mutation in several cancer cells. (bmbreports.org)
  • Pathological signs include gliosis, dystrophic neurites, vacuolated mitochondria, and accumulation of soluble hyperphosphorylated tau. (nih.gov)
  • DNA repair proteins that include the bacterial MutS DNA mismatch-binding protein and its eukaryotic homologs that function in DNA MISMATCH REPAIR and recombination of DNA during MEIOSIS. (bvsalud.org)
  • RNF213 is a giant E3 ubiquitin ligase and a major susceptibility factor of Moyamoya disease, a cerebrovascular disorder that can result in stroke or death. (biorxiv.org)
  • This is the crystal structure for o-succinylbenzoate CoA ligase in Staphylococcus aureus (strain N315) because the structure for E. coli has not been crystallized as of yet. (wikipedia.org)
  • Hypoxia and hypoxic states can occur in a range of diseases including in cancer. (mdpi.com)
  • Polymorphic alterations are also included. (lu.se)
  • The public release data file includes urinary chlamydia and gonorrhea data for participants aged 18-39. (cdc.gov)
  • Its goal is to achieve a comprehensive and objective global network, including direct (physical) as well as indirect (functional) interactions. (scienceopen.com)
  • Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly . (neb.com)
  • Mezigdomide is one of two cereblon E3 ligase modulators under development, the other being iberdomide (CC-220). (medpagetoday.com)
  • Recent reviews nicely describe our current understanding of the function of specific classes of ubiquitin ligases. (brookes.ac.uk)
  • L'ensemble nos études montrent que l'ubiquitination induite par les protéines CBL est un mécanisme de régulation important des lymphocytes B. Ces ubiquitines ligases contrôlent les différentes étapes de la réaction CG et d'une façon plus large la génération d'anticorps. (umontreal.ca)
  • However, a more holistic account of ubiquitin ligases in health and disease remain as an important pending assignment in the field. (brookes.ac.uk)
  • In addition, your package will include a detailed data report in both TSV and JSONL formats. (nih.gov)
  • The new studies are made possible by combining data from several breakthrough technologies, including in the fields of mass spectrometry and microscopy, which have been advanced in recent years at the MPI of Biochemistry. (izb-online.de)
  • The 101 patients included in data analysis had received a median of six prior lines of therapy, and three-fourths of the patients had undergone stem-cell transplantation. (medpagetoday.com)
  • The structures of the new compounds were elucidated from their spectroscopic and spectrometric data, including IR, HRESIMS, and NMR, and by comparison with spectroscopic data in the literature. (cancer.gov)
  • The public data file includes data for persons 18-39 years of age. (cdc.gov)
  • It depends on a stage-wise developmental process that includes B cell activation, antigen processing and presentation to T follicular helper (Tfh) cells, proliferation and somatic hypermutation in GC, selection and eventual differentiation into antibody-secreting plasma cells (PC) or memory B cells. (umontreal.ca)
  • The co-factor ATP is cleaved to pyrophosphate and AMP, with the AMP being covalently joined to a highly conserved lysine residue in the active site of the ligase. (embl.de)
  • In the cell, RNF213 was shown to be involved in fatty acid metabolism ( 11 ), and to associate with intracellular lipid droplets in a manner dependent on its E3 ligase and ATPase activities ( 12 ). (biorxiv.org)
  • We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or competent cell products. (neb.com)
  • Just rehydrate and add cells for RNAi screening of mouse F-box and SOCS box E3 ligases. (horizondiscovery.com)
  • This review focuses on an up-to-date understanding of the functions of E3 ligases in cancers and debates the perspectives of cancer cells that rely on inhibition or activation of ubiquitylation of target proteins. (bmbreports.org)
  • The search for targeted drugs for cancer treatment includes consideration of natural products, which may provide new opportunities for antitumor cytotoxicity as single agents or in combination therapy. (cancer.gov)