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  • Fertility
  • In vitro follicle techniques provide a tool to model follicle development in order to investigate basic biology, and are further being developed as a technique to preserve fertility in the clinic 1-4 . (jove.com)
  • The resulting increased incidence of aneuploidy likely accounts for the observed sub-fertility of mice harboring Hdac2 −/− oocytes. (prolekare.cz)
  • Human chorionic gonadotrophin priming for fertility treatment with in vitro maturation. (ox.ac.uk)
  • germ cell
  • Co-expression of cyp26a1 and the pluripotent germ cell stem cell marker pou5f1(oct4) in meiotically arrested oocytes was consistent with roles in mouse to promote germ cell survival and to prevent apoptosis, mechanisms that are central for tipping the sexual fate of gonads towards the female pathway in zebrafish. (jove.com)
  • mammalian
  • The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. (bioportfolio.com)
  • embryos
  • Procedures for in vitro fertilization (IVF) of rat oocytes were developed and validated by transferring the resulting embryos to recipient females. (uwo.ca)
  • Although embryos resulting from IVF were less successful in establishing pregnancy than appropriate in vivo fertilized controls, optimization of culture and transfer techniques enabled minimization of embyronic losses and permitted valid use of these techniques. (uwo.ca)
  • There is a delay in the developmental changes in glucose uptake, hexokinase activity and GLUT1 expression when the embryos are developed in vitro. (nii.ac.jp)
  • The oocytes collected were matured, fertilized with fresh ram semen and cultured in order to produce embryos. (ufs.ac.za)
  • gametes
  • Fertilization is the consequence of the fusion of two haploid gametes, the oocyte and the sperm. (bioportfolio.com)
  • Because generation of transgenic and gene-targeted mouse mutants is costly and can take long periods of time, manipulation of female gametes in vitro is a more economical and time-saving strategy. (jove.com)
  • recombinant
  • The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses. (bioportfolio.com)
  • In oocytes from hibernation-interrupted toads, the p34(cdc2) protein level was significantly lower than in oocytes from toads in artificial hibernation, providing an explanation for the different quantities isolated by recombinant tUCHL1 pull-down and, more importantly, identifying a mechanism involved in the toad oocytes dependence on a low environmental temperature during winter hibernation. (jove.com)
  • kinetochores
  • Most importantly, the spindle assembly checkpoint protein BubR1 could be detected on kinetochores even in oocytes cultured for 10 h. (jove.com)
  • The increased amount of acetylated H4K16 likely impairs kinetochore function in oocytes lacking HDAC2 because kinetochores in mutant oocytes are less able to form cold-stable microtubule attachments and less CENP-A is located at the centromere. (prolekare.cz)
  • spindle
  • tubulin on the spindle fibers and asters in oocytes after taxol treatment. (jove.com)
  • In this study, we showed that resveratrol, a kind of phytoalexin found in the skin of grapes, red wine and other botanical extracts, can alleviate the adverse effects caused by methylglyoxal, such as inhibition of oocyte maturation and disruption of spindle assembly. (jove.com)
  • oct4
  • The resulting OLCs express various markers consistent with oocytes such as Oct4 , Vasa , Bmp15 , and Scp3 . (jove.com)
  • cells
  • Volume 1 (Sperm and Oocyte Analysis) effectively describes how to manipulate mature germ cells, sperm and oocytes, from diverse organisms such as ascidians and pigs. (biologists.org)
  • But the understanding of how fertilization is achieved in vitro without a better understanding of how mature germ cells have arisen in the first place is unsatisfying to the developmental biologists among us. (biologists.org)
  • Culturing the normal cells as a primary culture in vitro will enable scientists to model specific changes that might lead to ovarian cancer in the distinct epithelium, thereby definitively determining the cell type of origin. (jove.com)
  • Maintaining normal cells in alginate hydrogels promotes short term in vitro culture of cells in their three-dimensional context and permits introduction of plasmid DNA, siRNA, and small molecules. (jove.com)
  • The soft agar colony formation assay is a well-established method for characterizing this capability in vitro and is considered to be one of the most stringent tests for malignant transformation in cells. (jove.com)
  • Tregs can be generated from naive CD4 T cells in vitro with TCR- and co-stimulation in the presence of TGFβ and IL-2. (jove.com)
  • Our aim was to develop and characterize a highly reproducible rat syngeneic in vitro model of the BBB using co-cultures of primary rat brain endothelial cells (RBEC) and astrocytes to study receptors involved in transcytosis across the endothelial cell monolayer. (jove.com)
  • Culturing cells in a three dimensional hydrogel environment is an important technique for developing constructs for tissue engineering as well as studying cellular responses under various culture conditions in vitro. (jove.com)
  • EGF is shown to stimulate maturation by increasing PG production in granulosa cells. (nii.ac.jp)
  • These cells could then be differentiated into the affected cell type of the patient and studied in vitro, or used to replace the damaged or missing cells. (jove.com)
  • The availability of various GFP-tagged proteins means that many cellular processes can be monitored in living cells over the course of minutes or hours, and using this technique, processes such as RNP transport, epithelial morphogenesis, and tissue remodeling have been described in great detail in Drosophila oocytes 1,2 . (jove.com)
  • Evidence is accumulating regarding the superiority of the latter technique due to decreased formation of ice crystals, which may be destructive to cells when thawing takes place as well as superior preservation of the ovarian stroma and vasculature," says Jani R. Jensen, M.D., director of the in vitro fertilization program at Mayo Clinic in Rochester, Minnesota. (mayoclinic.org)
  • culture
  • The alginate hydrogel floats in standard cell culture medium and supports the architecture of the tissue growth in vitro . (jove.com)
  • The oocytes classified as excellent and/or regular were grouped (10 oocytes/drop) in culture medium covered with mineral oil in disposable Petri dishes. (vin.com)
  • subsequently
  • Administration of pregnant mares' serum gonadotropin prior to oocyte collection increased the proportion of oocytes which subsequently underwent fertilization. (uwo.ca)
  • In Xenopus , manipulating gene function in cultured oocytes and subsequently fertilizing them can ameliorate these problems to some extent. (jove.com)
  • Processes
  • this allows one to combine genetic or pharmacologic perturbations with in vitro development to define the effects of such manipulations on the processes occurring during specific periods of development. (jove.com)
  • Standard microfabrication processes and bio-analytical techniques have been successfully utilized for decades in the computer and biological industries, respectively. (jove.com)
  • Further investigation of these processes revealed that methylglyoxal may affect the oocyte quality by resulting in excessive reactive oxygen species production, aberrant mitochondrial distribution and high level lipid peroxidation, and resveratrol can block these cytotoxic changes. (jove.com)
  • morphological
  • The oocytes were collected as described in study I. After appropriate slide fixation and preparation, the material was analyzed for morphological and ultrastructural characteristics and photographed. (vin.com)
  • mice
  • We report here that mice harboring Hdac1 −/+ / Hdac2 −/− or Hdac2 −/− oocytes are infertile or sub-fertile, respectively. (prolekare.cz)
  • conventional
  • We have applied a powerful and novel well-tempered ensemble parallel tempering (PT-WTE) technique along with conventional molecular dynamics (MD) simulation to investigate the molecular-level consequences of selective methionine oxidation of fibrinogen. (jove.com)
  • chromosome
  • Historically, chromosome-spreading techniques are used for counting chromosome number 6 . (jove.com)
  • Depleting maternal HDAC2 results in hyperacetylation of H4K16 as determined by immunocytochemistry-normal deacetylation of other lysine residues of histone H3 or H4 is observed-and defective chromosome condensation and segregation during oocyte maturation occurs in a sub-population of oocytes. (prolekare.cz)
  • In addition, the results provide additional support that histone deacetylation that occurs during oocyte maturation is critical for proper chromosome segregation. (prolekare.cz)
  • alginate
  • Previous in vitro results indicated that alginate beads might be a useful vehicle for food iron fortification. (jove.com)
  • females
  • Previous studies showed that the accumulation of methylglyoxal and advanced glycation ends can impair the oocyte maturation and reduce the oocyte quality in aged and diabetic females. (jove.com)
  • CONCLUSION
  • In conclusion, we provide a protocol for setting up an in vitro BBB model that is highly reproducible due to the quality assurance methods, and that is suitable for research on BBB transporters and receptors. (jove.com)
  • describes
  • This protocol describes a set of labeling techniques that can be used to gauge renal composition and test nephron functionality in the adult zebrafish kidney. (jove.com)
  • experiments
  • Previous antibody-blocking experiments have demonstrated that toad ubiquitin carboxyl-terminal hydrolase L1 (tUCHL1) is necessary for germinal vesicle breakdown during toad oocyte maturation. (jove.com)
  • In the context of MPF (maturation promoting factor) controlling oocyte maturation and to further understand the role of UCHL1 in oocyte maturation, we performed adsorption and co-immunoprecipitation experiments using toad oocyte protein extracts and determined that tUCHL1 is associated with MPF in toad oocytes. (jove.com)