• It contains all the information required for newcomers to achieve successful in situ hybridization results, and methods for improving the technique of those already utilizing it. (elsevier.com)
  • The application of some of these novel techniques has helped significantly extend the scope of utility of in situ hybridization in the fields of research and diagnostic pathology owing to their capability of detecting targets with low copy numbers of nucleic acids in samples. (openpr.com)
  • 12. The method according to claim 1, wherein the method further comprises a post-hybridization rinsing step consisting of three separate rinses, each having a duration of between 5 and 30 minutes. (google.com)
  • Spectral imaging was used for post hybridization tissue analysis, enabling separation of spatially colocalized signals. (broadinstitute.org)
  • We have used in situ hybridization to localize expression of the cystic fibrosis transmembrane conductance regulator (CFTR) gene in the human gastrointestinal tract and associated organs. (jci.org)