• Dr. Wang's research focuses on the application of peptide synthesis and FTIR spectroscopy in the pathology study of Alzheimer's disease and Parkinson's disease. (mtsu.edu)
  • In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. (biomedcentral.com)
  • Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. (biomedcentral.com)
  • Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process. (biomedcentral.com)
  • We provide thorough characterization of the effect of pH, effect of salts and protein conformation on self-assembled collagen samples via several complementary biophysical techniques, including circular dichroism (CD), Fourier Transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), atomic force microscopy (AFM), scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA) and thermogravimetric analysis (TGA). (biomedcentral.com)
  • They were identified as silk based on structures by morphological observation and chemical components revealed by Fourier Transform Infrared spectroscopy (FTIR). (springeropen.com)
  • Fourier-transformed infrared-vibration spectroscopy is capable to detect structure features on a molecular basis, including protein, carbohydrate, lipid related functional groups [ 1 ]. (biomedcentral.com)
  • The objective of this study was to investigate whether molecular structures that are related to protein and starch are highly affected by different heat processing methods using attenuated total reflectance-Fourier-transformed infrared-vibration spectroscopy (ATR-FT/IR) [ 20 ], and to study the heat-induced changes in nutrient profile and availability in relation to molecular structure changes in ruminants. (biomedcentral.com)
  • The structural and conformational changes of proteins are of great concern and several spectroscopic methods e.g. circular dichroism, 74-76 Fourier-transformed infrared spectroscopy (FTIR), 77,78 NMR, 79 small-angle X-ray scattering (SAXS) 80 and electron paramagnetic resonance 81 have been used. (azpdf.net)
  • Isotope-edited FTIR in H2O: Determination of the conformation of specific residues in. (mtsu.edu)
  • When peptides are in a beta-sheet conformation, particularly when the residues are parallel and in-register (causing alignment), glutamines can brace the structure by forming intrastrand hydrogen bonding between its amide carbonyls and nitrogens . (wikidoc.org)
  • Membrane binding may also promote rearrangement, dissociation, or conformational changes within many protein structural domains, resulting in an activation of their biological activity. (wikipedia.org)
  • In the present study, we explored the structural aspect of HomA and HomB proteins using various computational, biophysical and small-angle X-ray scattering (SAXS) techniques. (nature.com)
  • Our study provides essential structural information of unexplored proteins of the Hom family that can help in a better understanding of H. pylori pathogenesis. (nature.com)
  • MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)
  • The effects of different heat treatment conditions (65â for 30 min, 75â for 15 min, and 95â for 2, 15 and 30 min) on the evolution of off-flavor compounds in soy protein isolate (SPI) were investigated in terms of lipid oxidation, Maillard reaction and protein structural characteristics. (bvsalud.org)
  • After the spectroscopic characterization of the late rhodopsin photoproducts, we analyzed truncated variants and revealed the involvement of the cytosolic N-terminus in the structural rearrangements upon photo-activation of the protein. (elifesciences.org)
  • Collagen, the most abundant protein in the animal kingdom, represents a promising biomaterial for regenerative medicine applications due to its structural diversity and self-assembling complexity. (biomedcentral.com)
  • Collagen is one of the most important structural proteins, accounting for up to one quarter of protein biomass in mammals [ 1 ]. (biomedcentral.com)
  • Amyloids are insoluble fibrous protein aggregations sharing specific structural traits. (wikidoc.org)
  • E.A. Orabi and A.M. English ( 2018 ) Modeling protein S-aromatic motifs reveals their structural and redox flexibility, J. Phys Chem. (concordia.ca)
  • HPTS may be a good alternative to track structural transition of proteins at different pH and concentration. (azpdf.net)
  • Static electric field strengths of 0.001 V/nm and 0.002 V/nm induced conformational changes in the protein but had no significant effect on its surface properties. (mdpi.com)
  • These proteins may be anchored to the bilayer as a result of hydrophobic interactions between the bilayer and exposed nonpolar residues at the surface of a protein, by specific non-covalent binding interactions with regulatory lipids , or through their attachment to covalently bound lipid anchors. (wikipedia.org)
  • Other polypeptides and proteins , such as amylin and the Alzheimer's beta protein do not have a simple consensus sequence and are thought to operate by hydrophobic association. (wikidoc.org)
  • Among the hydrophobic residues, aromatic amino acids are found to have the highest amyloidogenic propensity. (wikidoc.org)
  • 82 The basic idea is that if the Trp is buried deep inside the hydrophobic protein moiety, it normally displays an emission maximum at 308 nm but whenever the protein becomes unfolded and the Trp residue is exposed to water and undergoes a red shifting to 355 nm. (azpdf.net)
  • A molecular dynamic (MD) modeling approach was applied to evaluate the effect of external electric field on gliadin protein structure and surface properties. (mdpi.com)
  • The described setup and experimental design enable real-time monitoring of substrate turnover in light-activated enzymes on a molecular scale, thus opening the pathway to a deeper understanding of enzyme activity and protein-protein interactions. (elifesciences.org)
  • Thus, understanding the molecular effects of aSyn point mutations may provide important insight into the molecular mechanisms underlying disease onset. (biomedcentral.com)
  • Transcriptomic analysis of the two recombinant strains in comparison with the control strain showed that the correct folding of proteins assisted by molecular chaperones played a key role in the improvement of FumDM expression and its enzyme activity. (inra.fr)
  • The objective of this study was to investigate the effect of heat processing methods on interaction between nutrient availability and molecular structure in terms of functional groups that are related to protein and starch inherent structure of oat grains with two continued years and three replication of each year. (biomedcentral.com)
  • The results reveal that oat grains are more sensitive to microwave irradiation than dry heating in terms of protein and starch molecular profile and nutrient availability in ruminants. (biomedcentral.com)
  • The ribosome is a molecular machine that catalyzes protein synthesis in three kingdoms of life. (itu.edu.tr)
  • In this thesis, residue interaction network, CG molecular dynamics (MD) simulations and anisotropic network model (ANM), which all use reduced representations of the structures based on one-bead coarse-graining, are employed. (itu.edu.tr)
  • The regulatory protein subunits of many ion channels and transmembrane receptors, for example, may be defined as peripheral membrane proteins. (wikipedia.org)
  • Some proteins, such as G-proteins and certain protein kinases, interact with transmembrane proteins and the lipid bilayer simultaneously. (wikipedia.org)
  • Some water-soluble proteins associate with lipid bilayers irreversibly and can form transmembrane alpha-helical or beta-barrel channels. (wikipedia.org)
  • Microbial rhodopsins (Rhs) are transmembrane proteins that utilize light-induced isomerization of their retinal cofactor to function as light-sensitive ion channels, pumps and sensors. (elifesciences.org)
  • obs, K. Dystrophin gene mutations that cause DMD result in either the absence of dystrophin protein production or markedly truncated proteins that cannot attach to the transmembrane protein com- plex and are rapidly catabolized. (top5binarybrokers.com)
  • The amount of B-side electron transfer after excitation at 390 nm has been observed to be altered in the HE(L168)/ND(L170) mutant in a pH-dependent manner that has been interpreted as arising from the presence of ionizable amino acids residues (Haffa et al. (trpv-antagonist.com)
  • It is affected by factors such as medium pH and by the presence of certain substances, for example cholesterol, other lipids, fatty acids, amines, amino acids, or proteins, incorporated in the lipid bilayer. (degruyter.com)
  • This facilitates the assembly of multi-protein complexes by increasing the probability of any appropriate protein-protein interactions. (wikipedia.org)
  • Further, biophysical experiments suggested that HomA and HomB are dimeric and most likely the cysteine residues present on surface-exposed loops participate in protein-protein interactions. (nature.com)
  • FLIM-FRET analysis of protein-protein interactions showed that PLIN5 S155 phosphorylation regulates PLIN5 interaction with adipose triglyceride lipase at the lipid droplet, but not with α-β hydrolase domain-containing 5. (uci.edu)
  • Arising from the reinforced intermolecular interactions upon chemical covalent bonds and physical hydrogen bonds, the maximum tensile strength and the elongation at break were increased from 10.61 MPa and 20.67 % for sodium carboxymethyl cellulose film to 42.15 MPa and 24.8 % for the optimized soybean protein isolate-graft-sodium carboxymethyl cellulose composite mulch film, respectively. (bvsalud.org)
  • FTIR analysis confirmed electrostatic and hydrogen bonding interactions between SPI and ChNCs. (bvsalud.org)
  • E.A. Orabi and A.M. English ( 2016 ) Sulfur-Aromatic Interactions: Modeling Cysteine and Methionine Binding toTyrosinate and Histidinium Ions to Assess Their Influence on Protein Electron Transfer , Isr. (concordia.ca)
  • ABSTRACT Self-templated protein aggregation and intracerebral deposi- tion of aggregates, sometimes in the form of amyloid fibrils, is a hallmark of mammalian prion diseases. (pdf-archive.com)
  • This led to protein unfolding, exposure of Trp residues, and orderly aggregation, forming a dense cross-linked gel network. (bvsalud.org)
  • Moreover, the accumulation of aSyn in Lewy Bodies and Lewy neurites in disorders such as PD, dementia with Lewy bodies, or multiple system atrophy, suggests aSyn misfolding and aggregation plays an important role in these disorders, collectively known as synucleinopathies. (biomedcentral.com)
  • These data suggest that the negatively charged glutamate residue may affect the folding and, consequently, the aggregation process of the protein. (biomedcentral.com)
  • Hatfield RD, Jung HJG, Ralph J, Buxton DR, Weimer PJ (1994) A comparison of the insoluble residues produced by the Klason lignin and acid detergent lignin procedures. (springer.com)
  • We detected phosphorylation on S155 and identified S155 as a functionally important site for lipid metabolism. (uci.edu)
  • M. Kathiresan and A.M. English ( 2018 ) LC-MS/MS proteoform profiling exposes cytochrome c peroxidase self-oxidation in mitochondria and functionally important hole hopping from its heme , J. Am. Chem. (concordia.ca)
  • More- over, internal water exchange is believed to be governed by confor- mational ¯uctuations in the protein and can therefore provide information about the thermal accessibility of functionally important con- formational substates. (lu.se)
  • Coupling within protein chains is also higher than coupling within rRNA chains indicating the more compact globular structures of proteins than rRNAs. (itu.edu.tr)
  • In terms of its structure, chitin is associated with proteins and, therefore, high in protein contents. (ukessays.com)
  • Perilipin 5 (PLIN5) is a lipid-droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and is thought to regulate lipid metabolism in response to phosphorylation by protein kinase A (PKA). (uci.edu)
  • The exact function of aSyn remains unclear, but it is known to be associated with vesicles and membranes, and to have an impact on important cellular functions such as intracellular trafficking and protein degradation systems, leading to cellular pathologies that can be readily studied in cell-based models. (biomedcentral.com)
  • Additionally, intracellular resting Ca 2+ level, which is important for gliotransmission, was also increased via TRPV1 on CNT platforms. (biomedcentral.com)
  • Here, we present a UV-Vis and FTIR study about the light-induced dynamics of a RGC from the fungus Catenaria anguillulae , which provides insights into the catalytic process. (elifesciences.org)
  • Furthermore, the tightly cross-linked structure of SPI-GA prevented premature degradation of the protein by pepsin. (bvsalud.org)
  • On the other hand, a problem remains, the rapidly and extensively degradation of their starch and protein in the rumen. (biomedcentral.com)
  • Biopolymers, such as proteins, DNA, and polysaccharides, which are available in almost unlimited quantity in nature, as well as many synthetic polymers, which are industrially produced on a large scale, belong to this fascinating class of polymers. (mdpi.com)
  • In order to map allosteric communications in the complexes, perturbation response scanning (PRS) and k-shortest pathways calculations are performed using the CGMD trajectories and the residue network model, respectively. (itu.edu.tr)
  • ANM using normal mode analysis, residue interaction network model and k-shortest pathways calculations are performed by in-house codes. (itu.edu.tr)
  • It also may involve the formation or dissociation of protein quaternary structures or oligomeric complexes, and specific binding of ions, ligands, or regulatory lipids. (wikipedia.org)
  • 0.01) was found between protein β-sheet and crude protein. (biomedcentral.com)
  • It also has moisture (25-30%), crude protein (2.9-6.6%), crude fiber (2.4-8.4%), crude fat (0.4-1.0%), and several other vitamins and minerals [ 3 ]. (springeropen.com)
  • The reversible attachment of proteins to biological membranes has shown to regulate cell signaling and many other important cellular events, through a variety of mechanisms. (wikipedia.org)
  • Under physiological conditions, aSyn is considered to be a pre-synaptic protein [ 37 ] that associates with vesicles and membranes [ 11 ]. (biomedcentral.com)
  • Targeting key residues of the PT-pathway by site directed mutagenesis significantly alters the pH-activity profile of these variants and in presence of H 2 their cofactor is trapped in an intermediate state indicative of precluded proton-transfer. (nature.com)
  • This component was assigned to direct proton transfer to proline (pro8) amino acid residue at the binding site of the protamine protein ( Figure 1.8 ). (azpdf.net)
  • Hence, what differentiates amyloids formed by bona fide prions from amyloids formed by other proteins is not well understood. (pdf-archive.com)
  • This study aimed to investigate the antimicrobial activity of vancomycin-loaded soy protein nanoparticles (vancomycin-SPNs). (hindawi.com)
  • Biocompatibility, biodegradability, and low toxicity are the most important properties of these nanoparticles [ 11 ]. (hindawi.com)
  • So, the aim of this study is the synthesis, characterization, and antibacterial evaluation of vancomycin-containing soy protein nanoparticles (vancomycin-SPNs) as a new antimicrobial agent. (hindawi.com)
  • HAP nanoparticles have been used as vehicles for delivery due to their affinity to DNA, proteins, several drugs, and proper release activity. (encyclopedia.pub)
  • The second derivative of DKCs spectra showed changes in the protein bands compared to mPSCs. (biomedcentral.com)
  • D. Martins and A.M. English ( 2015 ) Peroxynitrite and hydrogen peroxide elicit a similar cellular stress response mediated by the Ccp1 sensor protein, Free Rad. (concordia.ca)
  • Controlled drug delivery system is highly important for not only prolonged the efficacy of drug but also cellular development for tissue engineering. (biomedcentral.com)
  • Of the various functional components in Z. jujuba fruit, polysaccharide is especially important because of its bioactivities and large cellular concentrations. (springeropen.com)
  • Protein-based nanostructures are suitable compounds for drug delivery systems, especially antibiotic delivery systems [ 10 ]. (hindawi.com)
  • Some of the most recent advances in enzymatic glycosylation processes focused on the production of important bioactive compounds have been analyzed. (preprints.org)
  • This review focused on the important features of bitter apple mainly, bioactive compounds, their mode of action, medical applications, chemical characteristics, chemical compounds, and the latest research on the pharmacological interventions of bitter apple extracts. (biomedcentral.com)
  • Oat grains ( Avena sativa L.) are a good source of protein, unsaturated fatty acids, and phenolic compounds as well as dietary fibre and starch. (biomedcentral.com)
  • Finally, the principal components analysis obtained from FTIR spectra allowed to characterize chemical and structurally mPSCs and their differentiation process to DKCs in a rapid and non-invasive way. (biomedcentral.com)
  • The application of enzymes for waste treatment has been gaining pace commercially to solve concerns related to agricultural residues, wastewater, replacement of synthetic processes with natural ones, etc. (preprints.org)
  • Stem mechanical strength is an important agricultural quantitative trait that is closely related to lodging resistance in rice, which is known to be reduced by fertilizer with higher levels of nitrogen. (springer.com)
  • Current Issues There are also important problems in the development of CA4P concentration Family therapy in Poland. (dnarnasynthesis-signal.com)
  • Hydrogen, the simplest of molecules is also one of the most important industrial chemicals and more than 70 million tonnes are used worldwide. (ukri.org)
  • Some polypeptide hormones, antimicrobial peptides, and neurotoxins accumulate at the membrane surface prior to locating and interacting with their cell surface receptor targets, which may themselves be peripheral membrane proteins. (wikipedia.org)
  • While the locations and inferred H-bond connec- water molecules in a membrane protein, despite tivities of these internal water molecules are essen- being deeply buried and extensively H-bonded to tial, one would also like to quantify their mobility. (lu.se)
  • M.S. Shadrina, A.M. English and G.H. Peslherbe ( 2016 ) Benchmarking Rapid TLES Simulations of Gas Diffusion in Proteins: Mapping O 2 Migration and Escape in Myoglobin as a Case Study , J. Chem. (concordia.ca)
  • From the MRD data, the rotational correlation time of detergent-solubilized BR was determined to 35 ns at 300 K, con- sistent with a monomeric protein in complex with about 150 detergent molecules. (lu.se)
  • HPTS can directly transfer proton to proline (pro8) amino acid residue at the binding site even faster than in bulk water. (azpdf.net)
  • In addition, methods based on antigen-antibody immunoreactivity also play an important role in ancient silk analysis. (springeropen.com)
  • Cost-effective and user-friendly quantitation at points-of-need plays an important role in food safety inspection, environmental monitoring, and biomedical analysis. (inra.fr)
  • The analysis of E. coli crystal structure fluctuations has also shown that the protein components in the ribosomal complex are also in accord with experimental studies. (itu.edu.tr)
  • A mixed copper complex with deprotonated nalidixic acid (nal) and histamine (hsm) was synthesized and characterized by FTIR, UV-Vis, elemental analysis, and conductivity. (hindawi.com)
  • Outer membrane proteins (OMPs) of H. pylori play a crucial role in the host-pathogen interaction, virulence and pathogenesis. (nature.com)
  • k-shortest path technique is applied to residue interaction networks. (itu.edu.tr)
  • The results of these studies suggest that a higher superficial surface area leads to higher protein adsorption, which is also reported in other studies [ 10 ] . (encyclopedia.pub)
  • A higher protein α-helix to β-sheet and a lower amide I to starch area ratio were observed for MIO compared to DO and/or raw treatment. (biomedcentral.com)
  • LLO T223 is critical regarding phosphorylation with the inflammasome card ASC from amino residue Y144 by way of Lyn-Syk signaling, which can be important for ASC oligomerization. (dub-signal.com)
  • For fluorescence probing, the polarity sensitive nature of the tryptophan (Trp) residue is widely used. (azpdf.net)
  • ERY, marked as a risk prioritized macrolide antibiotic by 2015 released European Union watch list, most probably due to its protein inhibition capability considered third most widely used antibiotic. (afpm.org.my)
  • Leaves of the plant, flower petals, root systems, fruits, and fruit extract, seed residue, and seed pods can be used to prepare medicines, and herbal products and it can also be used in the food industry. (biomedcentral.com)
  • DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. (uci.edu)
  • Additionally, the positioning of many proteins are localized to either the inner or outer surfaces or leaflets of their resident membrane. (wikipedia.org)
  • H. pylori attaches and colonizes to the human epithelium using some of their outer membrane proteins (OMPs). (nature.com)
  • Hom (Helicobacter outer membrane) family of OMPs in H. pylori consists of four members (HomA, B, C and D). In the H. pylori genome jhp 0870 open-reading frame (ORF) that codes for HomB outer membrane protein is associated with many stomach diseases and is a novel co-marker for peptic ulcer disease (PUD) 9 , 10 . (nature.com)
  • Outer membrane proteins (OMPs) are distinctive features of Gram-negative bacteria. (nature.com)
  • In addition, experimental results showed that the optimized soybean protein isolate-graft-sodium carboxymethyl cellulose composite mulch film possesses soil moisture retention and controlled urea release properties. (bvsalud.org)
  • It also has ample phenol hydroxyl and epoxide residues on the basal planes, and carbonyl and carboxyl functional groups at the sheet edges [ 3 ]. (biomedcentral.com)
  • In conclusion, one important task for family therapists is ensuring a high level of therapeutic training and practice, and another important task is improving the position of family therapy in therapeutic treatment. (dnarnasynthesis-signal.com)
  • Scanning electron microscope (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), and FTIR were used for nanoparticle characterization. (hindawi.com)
  • Coenzyme Q10 is a biologically active compound that performs many important functions in the body: electron transfer in the mitochondrial cascade of adenosine triphosphate synthesis, antioxidant, preventing lipid peroxidation. (eco-vector.com)
  • This study demonstrated that it is necessary to gain insight into protein dynamics under external electric field stress, in order to develop the novel food processing techniques that can be potentially used to reduce or eradicate food allergens. (mdpi.com)
  • This study aimed to enhance the rheological properties and thermal stability of acid-induced soy protein isolate (SPI) gels by incorporating chitin nanocrystals (ChNCs) and proposing a gelation mechanism. (bvsalud.org)
  • In this study, we synthesized NPs from a medicinally important plant - Plumbago zeylanica . (dovepress.com)
  • Lipid-specific oligomerization of the Marburg virus matrix protein VP40 is regulated by two distinct interfaces for virion assembly. (uci.edu)
  • Gluten is a storage protein found in wheat, it plays an important role in regulating its baking quality by determining the viscosity, cohesivity, water absorption and elasticity of the dough [ 1 ]. (mdpi.com)
  • where they found that HAP nanopowders containing 100 nm particles adsorbed more proteins than 1 µm [ 9 ] . (encyclopedia.pub)
  • Copper in biological systems is usually found surrounded by imidazole moieties from histidine residues [ 13 , 14 ]. (hindawi.com)
  • The solubilized protein was found to be stable in the dark for at least eight months at 277 K. (lu.se)
  • What distinguishes amyloid fibrils formed by prions from those formed by other proteins is not clear. (pdf-archive.com)
  • Common to all forms of prions is the ability to form highly ordered protein aggregates, so-called amyloid fibrils. (pdf-archive.com)
  • Recent studies indicate that nanomechanics may play an important role not only in the conversion process of soluble proteins into their fibrillar state, but especially in the key characteristics of prions: their transmissibility.8,9 Amyloid fibrils are highly sensitive to local thermal fluctuations in liquid medium, which cause them to undergo bending along their longitudinal axis. (pdf-archive.com)
  • Glutamine-rich polypeptides are important in the amyloidogenesis of yeast and mammalian prions , as well as Huntington's disease . (wikidoc.org)
  • aSyn is a disordered and abundant neuronal protein whose normal function is still elusive. (biomedcentral.com)
  • These may include the folding of regions of protein structure that were previously unfolded or a re-arrangement in the folding or a refolding of the membrane-associated part of the proteins. (wikipedia.org)
  • Here we describe the 3.2 Å cryo-EM structure of human DEC-205, thereby illuminating the structure of the mannose receptor protein family. (uci.edu)
  • Ching A, Dhugga KS, Appenzeller L, Meeley R, Bourett TM, Howard RJ, Rafalski A (2006) Brittle stalk 2 encodes a putative glycosylphosphatidylinositol-anchored protein that affects mechanical strength of maize tissues by altering the composition and structure of secondary cell walls. (springer.com)
  • 73 In the protein protamine, an ESPT component of 65ps was observed which was significantly faster than the ESPT component (95ps) of HPTS in aqueous buffer. (azpdf.net)
  • The main components and pyrolysis characteristics of Camellia oleifera Abel hells, Castanea mollissima Blume shells, and Castanea mollissima Blume shells were analyzed by using FTIR and thermogravimetric methods. (scirp.org)