Assay reproducibility was initially assessed by random selection of the ANA 3+ or 4+ sera number of serum specimens tested and repeating the immunoprecipitation assay. (cdc.gov)
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is the most popular assay to identify genomic regions, called ChIP-seq peaks, that are bound in vivo by transcription factors (TFs). (nih.gov)
We screened serum samples for neutralizing antibodies by using a pseudotype microneutralization assay and a newly developed luciferase immunoprecipitation system assay. (cdc.gov)
Short for luciferase immunoprecipitation technology, LIPS is a traditional bait-and-bind diagnostic assay. (nih.gov)
Proteins10
Immunoprecipitation of intact protein complexes (i.e. antigen along with any proteins or ligands that are bound to it) is known as co-immunoprecipitation (Co-IP). (wikipedia.org)
As successive rounds of targeting and immunoprecipitations take place, the number of identified proteins may continue to grow. (wikipedia.org)
Chromatin immunoprecipitation (ChIP) is a method to investigate the association of proteins to specific genomic loci. (uni-muenchen.de)
The Magnetic Myc Immunoprecipitation Kit offers a simple, rapid method for purifying or immunoprecipitating Myc-tagged fusion proteins from cell lysates-using magnetic beads crosslinked to monoclonal anti-Myc antibody (which recognizes the Myc epitope). (scientifix.com.au)
Co-Immunoprecipitation, Co-IP) are used to study the interactions between the primary antigen protein and other proteins or nucleic acids (e.g. (ptglab.co.jp)
Chromatin Immunoprecipitation (ChIP) is a type of immunoprecipitation used to investigate regions of the genome associated with a target DNA-binding protein, or conversely to identify specific proteins associated with a particular region of the genome. (ptglab.co.jp)
Unlike co-Immunoprecipitation techniques that can probe protein interactions, ChIP can truly and completely reflect the regulatory information of target proteins bound to the promoter region of DNA, and it is a standard experimental technique to study DNA-protein interactions based on the whole genome level, and is also a commonly used method for detecting protein-DNA interactions in the natural chromatin environment in vivo. (kmdbioscience.com)
Co-immunoprecipitation of Argonaute proteins with associated RNAs. (cdc.gov)
Antibody9
Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. (wikipedia.org)
Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure. (wikipedia.org)
Antibody not able to perform in immunoprecipitation or used in too low amount. (agrisera.com)
IP is used to isolate a single protein (individual protein immunoprecipitation, the target antigen of the antibody). (ptglab.co.jp)
Individual Protein Immunoprecipitation uses an antibody to isolate a selected protein of interest from cell lysate. (ptglab.co.jp)
Human IgG antibody Laboratories manufactures the normal sheep igg polyclonal antibody control validated for use in immunoprecipitation reagents distributed by Genprice. (gentaurpriceonline.com)
The Normal Sheep Igg Polyclonal Antibody Control Validated For Use In Immunoprecipitation reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (gentaurpriceonline.com)
Detecting antibody reactivities in Phage ImmunoPrecipitation Sequencing data. (bvsalud.org)
Phage ImmunoPrecipitation Sequencing (PhIP-Seq) is a recently developed technology to assess antibody reactivity, quantifying antibody binding towards hundreds of thousands of candidate epitopes . (bvsalud.org)
Serologic assays for detection of antibodies against EBOV glycoprotein (EBOV-GP), matrix protein (VP40), and nucleoprotein (NP) included novel microneutralization and luciferase immunoprecipitation system (LIPS) assays, as well as a commercially available ELISA. (cdc.gov)
Assays1
Testing of IgG autoantibodies to human cellular antigens was performed on samples that showed a 3+ or greater immunofluorescence staining using immunoprecipitation assays by a standard method (Reeves WH,et al. (cdc.gov)
Sequencing9
In this protocol, we describe in detail the procedures to identify acetylated mRNA regions transcriptome-wide using acetylated RNAimmunoprecipitation and sequencing (acRIP-seq). (nih.gov)
We have conducted crosslinking, immunoprecipitation, and sequencing (CLIP-seq) to characterize N interactions with host and viral RNAs during infection. (mdpi.com)
Therefore, we conducted chromatinimmunoprecipitation sequencing (ChIP-seq) of TCF4 in neural-derived (SH-SY5Y) cells to identify genome-wide TCF4 binding sites, followed by data integration with SZ association findings. (biorxiv.org)
2. Quantitative Comparison of Multiple Chromatin Immunoprecipitation-Sequencing (ChIP-seq) Experiments with spikChIP. (nih.gov)
7. ChromatinImmunoprecipitation Sequencing (ChIP-seq) for Detecting Histone Modifications and Modifiers. (nih.gov)
8. ChromatinImmunoprecipitation and Sequencing (ChIP-seq) Optimized for Application in Caenorhabditis elegans. (nih.gov)
15. ChromatinImmunoprecipitation and High-Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis. (nih.gov)
16. A High-Throughput ChromatinImmunoprecipitation Sequencing Approach to Study the Role of MYC on the Epigenetic Landscape. (nih.gov)
18. ChromatinImmunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cardiac Tissue. (nih.gov)
Immunohistochemistry1
PA1-018 has been successfully used in Western blot, immunoprecipitation, immunofluorscence, immunocytochemistry and immunohistochemistry procedures. (thermofisher.com)
Purification3
At this point the immunoprecipitation is performed resulting in the purification of protein-DNA complexes. (wikipedia.org)
Immunoprecipitation is a method that enables the purification of a protein or antigen. (biologyexams4u.com)
ChIP techniques generally include cell fixation, chromatin breakage, chromatinimmunoprecipitation, reversal of cross-linking reactions, DNA purification and identification. (kmdbioscience.com)
Protocol1
Serum samples were tested using the standard immunoprecipitation protocol (Reeves WH,et al. (cdc.gov)
20231
Vancouver, B.C., June 19, 2023 - The global Immunoprecipitation (IP) market size was USD 675 Million in 2022 and is expected to register a steady revenue CAGR of 5.0% during the forecast period, according to latest analysis by Emergen Research. (emergenresearch.com)
Serum1
Detection of xenoestrogens in serum after immunoprecipitation of endogenous steroidal estrogens. (nih.gov)
20221
The ChromatinImmunoprecipitation (ChIP) segment accounted for significantly large revenue share in the global IP market in 2022. (emergenresearch.com)
Quantitative1
1. Native internally calibrated chromatinimmunoprecipitation for quantitative studies of histone post-translational modifications. (nih.gov)
Analyses1
Using methylated DNAimmunoprecipitation methodology in conjunction with promoter microarray analyses, we found that DKO cells experience a significant loss of hypermethylated CpG islands. (nih.gov)
Protein8
By repeating the immunoprecipitation in this way, the researcher verifies that each identified member of the protein complex was a valid identification. (wikipedia.org)
Chromatin immunoprecipitation (ChIP) is a method used to determine the location of DNAbinding sites on the genome for a particular protein of interest. (wikipedia.org)
Immunoprecipitation (IP), followed by Mass Spectrometry (MS), is a popular technique for analyzing protein complexes and effective method for determining protein-protein interactions. (emergenresearch.com)
Dynamic interactions between Protein A and Protein B were detected by co-immunoprecipitation using MagVigen™ nanobeads. (nvigen.com)
Immunoprecipitation (IP) is a precipitation technique that purifies and enriches a protein of interest, allowing the identification of protein-protein interactions in proteomics workflows. (ptglab.co.jp)
Co-Immunoprecipitation (Co-IP) is a powerful tool used to analyze protein-protein interactions. (ptglab.co.jp)
Chromatin Immunoprecipitation (ChIP) is a powerful tool for studying protein-DNA interactions in vivo, and it can be used not only to detect the dynamic interactions between trans factors and DNA in vivo, but also to study various covalent modifications of histones as well as the relationship between transcription factors and gene expression. (kmdbioscience.com)
Additionally put-miR-34, and put-miR-35 showed a high fold enrichment in argonaute-immunoprecipitation compared to the controls, indicating their ability to form a complex with argonaute protein and RNA induced silencing complex (RISC), a typical mode of action found with miRNAs. (cdc.gov)
Binding1
5. ChromatinImmunoprecipitation for Analyzing Transcription Factor Binding and Histone Modifications in Drosophila. (nih.gov)
Genes1
Thus, our results show the usefulness of DNMT genetic disruption strategies combined with methylated DNAimmunoprecipitation in searching for unknown hypermethylated candidate genes in human cancer that might aid our understanding of the biology of the disease and be of potential translational use. (nih.gov)
Identification1
Enhanced immunoprecipitation techniques for the identification of RNA-" by Saja A Fakhraldeen, Scott M Berry et al. (wustl.edu)
Technology1
This process combines the uses of single cell microfluidics and chromatinimmunoprecipitation (ChIP) for the analysis of DNA modifications in single cells.This technology has the potential to greatly decrease the number of cells needed from a biopsy to test for different cancers. (illinois.edu)
System1
13. ChromatinImmunoprecipitation in the Cnidarian Model System Exaiptasia diaphana. (nih.gov)