ImmunofluorescenceIndirectElectron microscopyImmunohistochemicalPolymerase chain reFrozen sectionsTissueBiopsyAntibodiesMethodsDiagnosticTumorEnzymeFindingsImmunohistochemistryDetectClinicalFoundCellsImagingNormalTreatmentArticleResultsAvidin-biotin immunoperoxidase techniqueAntigenAntibodyElectronLight microscopyLocalizationImmunoenzyme TechniquesParaffinHistochemistryTissuesLymphomasFluorescentDilutionDiagnosisLesionCellularMolecularObjectiveControlsSingleTotalCasesConditions
Immunofluorescence5
- Direct immunofluorescence monoclonal antibody staining and enzyme-linked immunosorbent assay (ELISA) are rapid and widely available detection techniques. (medscape.com)
- In forty-four patients with different prostatic lesions serum immunoglobulins and tissue deposited immunoglobulins were studied by single radial immunodiffusion technique, and direct immunofluorescence and immunoperoxidase (PAP) methods respectively. (jpgmonline.com)
- A series of monoclonal antibodies were used to study the intrathymic distribution of T cell-specific antigens, Ia antigens, and beta 2-microglobulin in frozen sections of human thymus by immunofluorescence and immunoperoxidase techniques. (rug.nl)
- However, unless special techniques such as immunofluorescence are used, or the infecting fungus possesses unique structures such as spherules, definitive identification of the etiologic agent by histopathology is difficult. (drfungus.org)
- The distribution of enkephalin-like immunoreactivity within the avian retina was studied by immunofluorescence and immunoperoxidase techniques with antiserum to [Met5]enkephalin and [Leu5]enkephalin. (shengsci.com)
Indirect6
- Detection of rickettsial antibodies using Weil-Felix (OXK and OX19) antigens and the indirect immunoperoxidase assay. (ajtmh.org)
- Serodiagnosis of tsutsugamushi fever (scrub typhus) by the indirect immunoperoxidase technique. (ajtmh.org)
- Summary The presence and localization of fibronectin in normal and mechanically injured aorta in rabbits was studied using an indirect immunoperoxidase technique on tissue specimens fixed in formaldehyde, embedded in paraffin and pretreated with pepsin. (zib.de)
- Immunostaining using the indirect immunoperoxidase or amino acid polymer method and non-isotopic in situ hybridization technique were employed when necessary. (pathos223.com)
- The indirect immunoperoxidase method using 1:500 to 1:1,000 diluted patients f serum as a primary antibody was also applied to localize pathogens within the lesion fixed in 10-20% formalin and embedded in paraffin. (pathos223.com)
- EGF receptors were identified on frozen sections by means of an indirect immunoperoxidase technique with a monoclonal antibody against the EGF receptor. (ox.ac.uk)
Electron microscopy1
- Alternative methods include the use of immunoperoxidase, electron microscopy, and polymerase chain reaction (PCR) assay. (medscape.com)
Immunohistochemical2
- In particular, immunoperoxidase reactions refer to a sub-class of immunohistochemical or immunocytochemical procedures in which the antibodies are visualized via a peroxidase-catalyzed reaction. (wikipedia.org)
- Despite several evaluated the diagnostic value of immunohistochemical clinical descriptions of ATBF, the pathologic features of techniques by using a monoclonal antibody to R. africae . (cdc.gov)
Polymerase chain re1
- Molecular techniques like polymerase chain reaction (PCR) or gene sequencing. (stemcelldaily.com)
Frozen sections1
- The distribution of neoplastic and normal B-lymphoid cells in nodular lymphomas: use of an immunoperoxidase technique on frozen sections. (bdbiosciences.com)
Tissue4
- The first step in immunoperoxidase staining is the binding of the specific (primary) antibody to the cell or tissue sample. (wikipedia.org)
- METHODS Synovial tissue specimens from 40 patients with RA and eight patients with non-rheumatic disease were obtained by arthroscopy guided biopsy techniques or during joint surgery. (bmj.com)
- Techniques based on immunohistochemistry have thus also been widely used to study the expression of cytokines within the synovial tissue in attempts to determine true local production. (bmj.com)
- Tissue typing was undertaken using a standard microcytotoxicity technique. (ox.ac.uk)
Biopsy1
- immunoperoxidase techniques parathyroid neoplasms thyroid neoplasms aspiration biopsy Acta Cytologica Immunoperoxidase Staining. (karger.com)
Antibodies1
- Serum anticolon and portal tract antibodies were detected using immunoperoxidase techniques on normal colon and obstructed human liver. (ox.ac.uk)
Methods1
- METHODS: An immunoperoxidase technique was used to visualize the presence of alpha 2-macroglobulin in the corneas. (arvojournals.org)
Diagnostic4
- A field survey of hematopoietic neoplasia (Hn) in the soft shell clamMya arenaria (L.) was undertaken using an immunoperoxidase diagnostic technique. (firebaseapp.com)
- For conventional US technique, it still remains a diagnostic challenge to identify the type of FLLs precisely and to distinguish between HCC and non-HCC, especially in the presence of chronic liver disease or cirrhosis. (biomedcentral.com)
- At least in part, the relative paucity of reports probably reflects the lack of sophisticated fungal diagnostic techniques in much of Southeast Asia. (cdc.gov)
- US$2/day ( 10 ) and one accessible microbiologic culture laboratory in Laos, PCR is not an available local routine diagnostic technique. (cdc.gov)
Tumor1
- After the breakthrough discovery of tumor control cells, analysts attempted to appearance for effective techniques to focus on cancers control cells. (immune-source.com)
Enzyme1
- In an immunoperoxidase procedure, an enzyme known as a peroxidase is used to catalyze a chemical reaction to produce a coloured product. (wikipedia.org)
Findings1
- These findings should be verified using techniques that can detect smaller genetic changes, such as microarray-CGH. (bmj.com)
Immunohistochemistry2
- Human and rat dental pulp under normal and inflammatory conditions were analyzed using toluidine blue histochemistry and immunohistochemistry techniques. (bvsalud.org)
- Therefore, the purpose of the present work was, under the same experimental conditions, to identify mast cells in healthy and inflamed dental pulp from rats and humans using toluidine blue histochemistry and immunohistochemistry techniques. (bvsalud.org)
Detect1
- Superb Microvascular Imaging (SMI) is an innovative vascular imaging technique that can detect microvascular flow at low velocity without contrast enhancement [ 6 ]. (biomedcentral.com)
Clinical2
- Immunoperoxidase is a type of immunostain used in molecular biology, medical research, and clinical diagnostics. (wikipedia.org)
- Immunoperoxidase staining is used in clinical diagnostics and in laboratory research. (wikipedia.org)
Found1
- Cysts of Toxoplasma gondii found in the brain of a beluga using an immunoperoxidase technique. (healthywildlife.ca)
Cells1
- The staining of cells in the medulla was most satisfactorily judged in sections stained with the immunoperoxidase technique. (rug.nl)
Imaging1
- Imaging techniques like X-rays, ultrasound or MRI. (stemcelldaily.com)
Normal1
- Treatment with testicular hyaluronidase before immunoperoxidase staining resulted in a higher staining resolution in normal and injured aorta. (zib.de)
Treatment1
- The effect on staining quality of treatment with testicular hyaluronidase prior to immunoperoxidase staining was also examined. (zib.de)
Article1
- A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
Results1
- These systems provide sensitive results, but the special handling and disposal problems of 125 I reagents have discouraged continued use of this technique. (bio-rad.com)
Avidin-biotin immunoperoxidase technique3
- A standard avidin-biotin immunoperoxidase technique [ 26 ] was used. (medscape.com)
- Paraffin wax sections of 70 surgically resected colorectal adenocarcinomas were examined for the overexpression of HER2/c-erbB-2 oncoprotein using three different specific antibodies and the avidin-biotin immunoperoxidase technique. (ox.ac.uk)
- Sections of 149 breast carcinomas were examined for the over-expression of c-erbB-2 oncoprotein using the avidin-biotin immunoperoxidase technique and two different specific antibodies. (ox.ac.uk)
Antigen6
- Mason-Pfizer monkey virus-related antigen was detected in 3 out of 5 jaagsiekte lungs examined using a direct immunoperoxidase staining technique with anti-MPMV p27 serum. (up.ac.za)
- Confirmation of the endothelial origin of these tumors was provided by positive immunoperoxidase staining for Factor-VIII-related antigen in the four cases studied by that technique, and by the demonstration of Weibel-Palade bodies in two tumors examined by electron microscopy. (nih.gov)
- Paraffin-embedded sections of brain were examined for morbillivirus antigen by using an immunoperoxidase technique. (cdc.gov)
- Using immunoperoxidase staining and antigen liberation techniques, both apical and basolateral RhCG localization was observed in the majority of the cells of the distal convoluted tubule and IC of the connecting tubule and collecting duct. (bris.ac.uk)
- An immunoperoxidase technique using a polyclonal anti-ASFV serum was performed on tissue sections in order to detect ASFV antigen. (ulpgc.es)
- Eighty percent of them had specific CMV IgG antibodies in the sera, as determined by the immunoperoxidase antibody to membrane antigen (IPAMA) technique. (bgu.ac.il)
Antibody10
- The first step in immunoperoxidase staining is the binding of the specific (primary) antibody to the cell or tissue sample. (wikipedia.org)
- Despite several evaluated the diagnostic value of immunohistochemical clinical descriptions of ATBF, the pathologic features of techniques by using a monoclonal antibody to R. africae . (cdc.gov)
- Antibody binding was detected using both immunoperoxidase and light microscopy on tissue sections, and 10 nm gold conjugated secondary antibody and electron microscopy on ultrathin sections. (nih.gov)
- Mumps primary antibody--applied to both mumps infected vero cells, and measles infected vero cells and tissues studied by immunoperoxidase, and measles antibody on mumps infected cells studied by immunoperoxidase and immunogold--were used as specificity controls: the primary antibodies identified their respective target antigen and there was no antibody cross reactivity. (nih.gov)
- The expression was detected using indirect fluorescent antibody, immunoperoxidase and SDS-PAGE/western blot techniques. (lbp.world)
- Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Content", "Value": "One dispenser of this antibody contains sufficient prediluted reagent for 50 tests. (roche.com)
- Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Intended Use", "Value": "IgM Rabbit Polyclonal Primary Antibody is intended for laboratory use in the detection of IgM in formalin-fixed, paraffin-embedded tissue stained on VENTANA BenchMark IHC/ISH instruments. (roche.com)
- Language": "en", "Country": "XG", "Code": "Intended Use" }, { "Name": "Principle", "Value": "IgM Rabbit Polyclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. (roche.com)
- The indirect peroxidase labelled antibody method was as specific and sensitive as the PAP technique. (kuhlmann-biomed.de)
- Methods: 140 formalin-fixed paraffin-embedded sections of bone tumours and tumour-like lesions were stained by an indirect immunoperoxidase technique with a polyclonal anti-periostin antibody. (ox.ac.uk)
Electron5
- Urine samples were collected into clean tubes for cytopathologic diagnosis, immunocytochemical techniques, and electron microscopic examination using agarose cell block technique in addition to the immunoelectronmicroscopic examination. (medscape.com)
- These cells were prepared for immunocytochemical techniques and electron microscopic examination using agarose cell block technique in addition to the immunoelectronmicroscopic examination. (medscape.com)
- The hematopathology rotation includes lymph node examinations, bone marrow core biopsies, fine needle aspiration biopsies, surface marker studies including immunofluorescence and immunoperoxidase techniques, flow cytometry, electron microscopy, DNA ploidy analysis and molecular genetic applications. (amc.edu)
- Immunoperoxidase light microscopy and immunogold electron microscopy revealed that the increase in kidney GSTM1 and GSTP1 occurred in nuclei, cytoplasm and microvilli of proximal tubules. (nih.gov)
- Protocollagen proline hydroxylase was purified from chick embryos, and it was examined by electron microscopy with negative staining techniques. (harvard.edu)
Light microscopy1
- Related techniques are also useful in sub-typing lymphocytes which all look quite similar on light microscopy. (wikipedia.org)
Localization1
- Analysis of the effects of lead on GST mRNA and GST cellular localization were performed by Northern blot and immunohistochemical techniques. (nih.gov)
Immunoenzyme Techniques3
- Immunoenzyme Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
- This graph shows the total number of publications written about "Immunoenzyme Techniques" by people in this website by year, and whether "Immunoenzyme Techniques" was a major or minor topic of these publications. (rush.edu)
- Below are the most recent publications written about "Immunoenzyme Techniques" by people in Profiles. (rush.edu)
Paraffin1
- Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded bovine cartilage. (novotec-labs.com)
Histochemistry2
- Human and rat dental pulp under normal and inflammatory conditions were analyzed using toluidine blue histochemistry and immunohistochemistry techniques. (bvsalud.org)
- Therefore, the purpose of the present work was, under the same experimental conditions, to identify mast cells in healthy and inflamed dental pulp from rats and humans using toluidine blue histochemistry and immunohistochemistry techniques. (bvsalud.org)
Tissues1
- The techniques were validated using both measles infected vero cells and human tissues with established measles infection: these included brain affected by subacute sclerosing panencephalitis and acute measles appendicitis. (nih.gov)
Lymphomas2
- The distribution of neoplastic and normal B-lymphoid cells in nodular lymphomas: use of an immunoperoxidase technique on frozen sections. (bdbiosciences.com)
- In the 1970s, people began to apply immunologic tools and concepts to the study of lymphomas but those techniques could not be widely used, and there was a lot of controversy over lymphoma classification, with half a dozen different classification schemes being used. (nih.gov)
Fluorescent1
- The fellow will also be exposed to molecular cytogenetics techniques using fluorescent in-situ hybridization (FISH). (amc.edu)
Dilution1
- In this study we have used an agar dilution technique to test the effect of six other dicarboxylic acids (sebacic, undecanedioic, dodecanedioic, tridecanedioic, tetradecanedioic and hexadecanedioic acid, 10(-4)-10(-2) mol/l, pH 5.5) on in vitro growth of Trichophyton (T.) rubrum, T. mentagrophytes and Microsporum (M.) canis. (medicaljournals.se)
Diagnosis1
- The alpha-bungarotoxin-immunoperoxidase technique already has shown promise for the diagnosis and analysis of mechanisms in human neuromuscular disorders. (nih.gov)
Lesion2
- B, CCND1-positive staining (brown by immunoperoxidase) in the nuclei of BE lesion cells from a patient who was germline MSR1 -mutation positive. (jamanetwork.com)
- Hepatic Magnetic Resonance Imagery with diffusion technique objective of atypical hemangioma lesion. (biomedcentral.com)
Cellular1
- Suitable for: Standard PAP smear tests (excellent cellular adhesion in cytocentrifugal preparations) Immunoperoxidase hybridisations In- situ hybridisations of DNA Sale only to companies. (eu.com)
Molecular1
- There is exposure to molecular diagnostic techniques including DNA extraction, gel electrophoresis, Southern transfer, probe hybridization and the polymerase chain reaction. (amc.edu)
Objective1
- However, in more recent years, the advent of more objective techniques (eg, immnoassays, nucleic acid amplification techniques [NAATs]) has led to an increase in their use versus those that rely on subjective microscopic examination of fecal specimens for Giardia cysts. (medscape.com)
Controls1
- The distribution of lysozyme was investigated in psoriatic skin lesions, perilesional skin and in skin from healthy controls, using the immunoperoxidase techniques avidin-biotin complex and alkaline phosphatase-anti alkaline phosphatase. (medicaljournals.se)
Single1
- Studies by Eisensehr et al using iodine 123 ( 123 I) immunoperoxidase technique (IPT) single photon-emission computed tomography (SPECT) scanning demonstrated that striatal presynaptic dopamine transporters are reduced in idiopathic RBD. (medscape.com)
Total1
- Therapeutic strategy was reconsidered and total gastrectomy with extended D1.5 lymph node dissections, Roux-en-Y esophagojejunostomygastric surgery was practiced in August 2010. (biomedcentral.com)
Cases1
- The technique was applied subsequently to six untreated cases of granulomatous Crohn's disease, and two cases of ileocaecal tuberculosis, a granulomatous control. (nih.gov)
Conditions1
- Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Background Information", "Value": "Anti-IgM reacts with immunoglobulin mu (IgM) chains. (roche.com)