AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureHealth Care
Immunomagnetic SeparationNeoplastic Cells, CirculatingMagneticsCell SeparationSensitivity and SpecificityCryptosporidium parvumFlow CytometryPolymerase Chain ReactionEscherichia coli O157Antigens, CD34OocystsLeukapheresisBiological MarkersMicrospheresImmunologic TechniquesFecesChymopapainGenetic MarkersBone Marrow PurgingAntibodies, MonoclonalHematopoietic Stem CellsAntigens, CDEvaluation Studies as TopicBone Marrow NeoplasmsBacteriological TechniquesFood MicrobiologyTumor Markers, BiologicalImmunophenotypingPlant PathologyEnzyme-Linked Immunosorbent AssayMagnetsHorseradish PeroxidaseMagnetospirillumMagnetite Nanoparticles
Foodborne pathogensLiquid biopsyBeadEpithelialBiomarker detectionEnrichmentCytokinesBeadsWidelyTumor cellsExosomesPathogenicAdvantagesReceptorsRapid detectionAssayAntigenAntibodyDistinctMolecularIsolationMethodsSerumParticlesOpticalCD14ProgressionVersatileSignalFlowAnalysisMethodCellLimitExpressBloodPotentialEarlyPlatformReview
Foodborne pathogens3
  • In-situ fluorescent immunomagnetic multiplex detection of foodborne pathogens in very low numbers. (docksci.com)
  • comprehensive pathogen detection methods will enable implementation of effective strategies to avoid, or when needed intervene in, the distribution of foods that have been contaminated with foodborne pathogens. (docksci.com)
  • There are two formats in each immunoassay test: 1) sandwich format (for macromolecule detection, such as foodborne pathogens and allergen proteins). (peertechzpublications.com)
Liquid biopsy2
  • The detection of CTCs, or liquid biopsy, presents several advantages over traditional tissue biopsies. (wikipedia.org)
  • The aim of this thesis was to realize single cell isolation by means of automated immunomagnetic bead enrichment and antibody-based fluorescence detection in a chip-based microfluidic system and the molecular biological characterization of these cells to assay tumor features from liquid biopsy. (fraunhofer.de)
Bead3
  • EVs were characterized by transmission electron microscopy and fluorescence nanoparticle tracking analysis, and EV surface protein profiles were analyzed by multiplex bead-based flow cytometry to identify tumor- or immune system-related EVs of AML, ALL, CLL, and healthy samples. (nature.com)
  • Therefore, this work compares manual and automated immunomagnetic enrichment in terms of cell recovery in dependency of cell line and input number, bead characteristics, biofunctionaliza-tion protocol and blood cell contamination focusing on head and neck squamous cell carcinoma (HNSCC) and metastatic breast cancer. (fraunhofer.de)
  • We highlighted the bottlenecks of microfluidic cell isolation in dependency of bead-binding on the cell surface and implemented a gating function for the cytometer subunit to selectively dispense cells instead of autofluorescent objects. (fraunhofer.de)
Epithelial2
  • CTCs that originate from carcinomas (cancers of epithelial origin, which are the most prevalent) can be classified according to the expression of epithelial markers, as well as their size and whether they are apoptotic. (wikipedia.org)
  • This method uses either epithelial cell-specific markers which are generally expressed in all tumor cells, or tumor markers expressed by specific types of cancer ( 13 ). (iiarjournals.org)
Biomarker detection1
  • Since blood plasma circulates through the whole body, it is often the biofluid of choice for biomarker detection in EVs. (biomedcentral.com)
Enrichment2
  • In the microfluidic benchtop platform CTCelect, we characterized each process step with two different carcinoma cell lines (MCF-7, SCL-1) demon-strating up to 87 Percent enrichment, 73 percent optical detection and dispensing efficiency. (fraunhofer.de)
  • Fluorescent EV spike recovery evidenced that target EV subpopulations can be efficiently retrieved from plasma, and that their enrichment is dependent not only on complex matrix composition, but also on the EV surface phenotype. (biomedcentral.com)
Cytokines2
  • Compared with controls, the detectable proportion of HIV-1 particles derived from CD14 macrophages and CD26 lymphocytes was increased in persons with acute malaria coinfection and correlated with markedly increased plasma concentrations of both proinflammatory cytokines and soluble markers of macrophage and lymphocyte activation. (lww.com)
  • They are more heterogeneous than normal fibroblasts and express various surface receptors and cytokines that facilitate tumor progression, angiogenesis, etc. [ 13 ]. (por-journal.com)
Beads3
  • Beads with covalently-conjugated antibodies on their surface outperformed streptavidin-conjugated beads, coated with biotinylated antibodies, in EV immunoprecipitation. (biomedcentral.com)
  • Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G. Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution. (thenewsindependent.com)
  • Potential subpopulations of exosomes can be captured by targeting these markers using magnetic beads. (exosome-rna.com)
Widely3
  • Immunological techniques, which are the most widely used methods, employ immunomagnetic isolation ( 4 ). (iiarjournals.org)
  • Epitope tags and anti-epitope tag antibodies are widely used for purification and detection of recombinant proteins in immunoblotting, immunostaining and immunoprecipitation. (thenewsindependent.com)
  • The study of ancient silk dyeing processes has also gained significant attention, with LC-MS/MS being the most widely utilized detection technique. (springeropen.com)
Tumor cells1
  • In doing so, CTCelect enabled automated dispensing of single circulating tumor cells from HNSCC patient samples, qPCR-based confirmation of tumor-related biomarkers with a simplified one-step protocol and immunostaining using a panel of tumor markers. (fraunhofer.de)
Exosomes1
  • Exosomes are here defined as extracellular vesicles (EVs) in the approximate size range of 30-100 nm in diameter, and are observed in most body fluids containing typical exosomal markers such as CD9, CD63, and CD81. (exosome-rna.com)
Pathogenic4
  • The complexity of food matrices, the wide variety of microorganisms therein, and the varying growth and replication traits of target pathogenic microorganisms that, when present, are often in much smaller numbers than the nonpathogenic microorganisms, pose significant challenges for pathogen detection. (docksci.com)
  • Although these biosensors are currently among the less popular tools for human disease detection, they have become useful tools for the screening and detection of pathogenic bacteria, fungi, and viruses because of their versatile advantageous features compared to other sensing devices. (frontiersin.org)
  • The application of molecular techniques in food is large, several authors comment on the detection and quantification of strains of pathogenic microorganisms present in foods and the presence of toxins and waste allergens not related to specific goods in production lines. (peertechzpublications.com)
  • In general, the most known and used methods are Western Blots (WB), Enzyme-Linked Immune Sorbent Assay (ELISA), Lateral-Flow Assay (LFA), and Dot-Blot Assay (DBA) and have been applied in the detection of carcinogenic compounds, pathogenic and allergenic microorganisms using antibodies [8]. (peertechzpublications.com)
Advantages2
  • The developed in-situ fluorescent immunomagnetic sensor approach offers distinct advantages because it is rapid, highly sensitive, and easy to use and could therefore be potentially used as a pathogen screening tool. (docksci.com)
  • The purpose of this review is to provide a complete update of the various analytical methods for virus detection, such as molecular, immunological, sensor-based and other detection assays, along with the advantages and limitations of these strategies. (frontiersin.org)
Receptors3
  • In conclusion, EMP-PDI promotes early platelet activation through glycoprotein (GP) IIb/IIIa receptors present on platelet surface in the diabetic state. (aging-us.com)
  • However, the ultimate common pathway of platelet aggregation is the activation of glycoprotein (GP) IIb/IIIa receptors present on platelet surface [ 7 - 9 ]. (aging-us.com)
  • Lymphocytes are recruited to tissues from blood via binding to vessels in the target tissue mediated by interactions between specific receptors on the lymphocyte surface and ligands on the endothelium. (biomedcentral.com)
Rapid detection3
  • A simple fluorometric immunological method in combination with a magnetic concentration step was developed for rapid detection of target bacteria with high sensitivity and specificity in less than 2 h without enumeration. (docksci.com)
  • This review summarizes the currently available methods employed for the rapid detection of ZIKV and suggests an innovative approach involving the application of a liposome-based hypothesis for the development of new strategies for ZIKV detection and their use as effective biomedicinal tools. (frontiersin.org)
  • Considering the calamity of ZIKV infection, there is an urgent need to develop rapid detection methods for ZIKV along with DENV, which shares common clinical symptoms with ZIKV. (frontiersin.org)
Assay1
  • 2005. Development of a species-specific and sensitive detection assay for Phytophthora infestans and its application for monitoring of inoculum in tubers and soil . (hutton.ac.uk)
Antigen1
  • We produced a fluorescent antibody as a single recombinant protein in Escherichia coli by fusing a red-shifted mutant of green fluorescent protein (EGFP) to a single-chain antibody variable fragment (scFv) specific for hepatitis B surface antigen (HepBsAg). (thenewsindependent.com)
Antibody1
  • The MoP9 antibody reacts with CD14, a 53 - 55 kDa glycosylphosphatidylinositol (GPI)-anchored transmembrane glycoprotein expressed at high levels on the surface of peripheral blood monocytes and macrophages, and at lower levels on granulocytes. (stemcell.com)
Distinct1
  • Finally, mRNA profiling experiments proved that distinct EV subpopulations can be captured by directly targeting different surface markers. (biomedcentral.com)
Molecular3
  • En el presente reporte se hizo una caracterización del polirribosilribitol fosfato mediante la técnica de cromatografía de exclusión molecular de alta eficacia con detección ultravioleta a 215 nm. (bvsalud.org)
  • In the present report a characterization of polyribosylribitol phosphate was performed by high performance molecular exclusion chromatography with ultraviolet detection at 215 nm. (bvsalud.org)
  • Molecular techniques have facilitated the identification, genotyping, and monitoring of food contamination sources, identification of genetically modified organisms, and use as food detection methods to avoid falsification through genetic markers. (peertechzpublications.com)
Isolation1
  • Plasma-derived EVs were captured and detected using this straightforward protocol, sequentially combining isolation and staining of specific surface markers, such as CD9 or CD41. (biomedcentral.com)
Methods4
  • This regulation requires enhanced detection of pathogens in both imported and domestically produced foods coupled with effective intervention methods to achieve food safety. (docksci.com)
  • Hence, this review reports possible methods and strategies for the fast and reliable detection of ZIKV with particular emphasis on current updates, knowledge, and new hypotheses that might be helpful for medical professionals in poor and developing countries that urgently need to address this problem. (frontiersin.org)
  • In this review, the current and novel methods for detection of breast cancer metastasis, as well as the mechanisms involved in metastasis and the treatment of breast cancer metastasis, are discussed. (iiarjournals.org)
  • Currently, detection of breast cancer metastasis relies on clinical manifestations of the spread to distant organs, biopsies of affected organs, radiological evaluations, imaging methods and serum tumor markers ( 3 , 4 ). (iiarjournals.org)
Serum3
  • Special EV surface protein patterns in serum have the potential as noninvasive biomarker candidates to distinguish several disease-related patterns ex vivo or in vivo. (nature.com)
  • Aiming to provide proof-of-concept evidence and methodology for the potential role of serum-derived EVs as biomarkers in leukemic versus healthy samples in this study, we hope to pave the way for future detection of promising biomarkers for imminent disease progression and the identification of potential targets to be used in a therapeutic strategy. (nature.com)
  • 6 ) emphasized that the inclusion of serum tumor markers is an important factor in the postoperative monitoring of breast cancer patients ( 7 , 8 ). (iiarjournals.org)
Particles2
  • Immunomagnetic HIV-1 capture analysis was used to determine the cellular origin of cell-free virus particles present in all 30 plasma samples and indices of immune activation were measured using enzyme-linked immunosorbent assays. (lww.com)
  • Parasite clearance following treatment with antimalarial drugs resulted in decreased detection of HIV-1 particles derived from the CD14 macrophage cell subset and correlated with a marked diminution in systemic immune activation. (lww.com)
Optical2
  • Optical detection technologies used for many types of biological and clinical analysis can play a significant role in addressing this need, but must be sufficiently affordable and portable for use in global health settings. (mdpi.com)
  • Metronomic (that is, low-dose and long-term) photodynamic therapy (mPDT) for treating internal lesions requires the stable fixation of optical devices to internal tissue surfaces to enable continuous, local light delivery. (regenerativemedicine.net)
CD141
  • An approximate 10-fold difference in expression levels between monocytes/macrophages and granulocytes makes CD14 a useful marker for distinguishing these cell populations. (stemcell.com)
Progression1
  • Detection of breast cancer metastasis at the earliest stage is important for the management and prediction of breast cancer progression. (iiarjournals.org)
Versatile1
  • GFP is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. (thenewsindependent.com)
Signal1
  • iTRAQ, SWATH, Label-free, PRM, various quantitative proteomic technologies to assist you in discovering key proteins of signal transduction pathways, discovering disease markers, and discovering drug targets. (creative-proteomics.com)
Flow1
  • Flow cytometric detection of CD44 (H-CAM) in hairy cell leukemia. (ntu.ac.uk)
Analysis2
  • The detection and analysis of CTCs can assist early patient prognoses and determine appropriate tailored treatments. (wikipedia.org)
  • For example, analysis of blood samples from cancer patients has found a propensity for increased CTC detection as the disease progresses. (wikipedia.org)
Method1
  • Currently, there is one FDA-approved method for CTC detection, CellSearch, which is used to diagnose breast, colorectal and prostate cancer. (wikipedia.org)
Cell1
  • VAP-1 mediates adhesion via heavily sialidated side chains but it also functions as a cell surface expressed ectoenzyme-a primary amine oxidase. (biomedcentral.com)
Limit2
  • The limit of detection (LOD) of IgG was 3.4 ng/mL. (biomedcentral.com)
  • Expressing a positive analytical measurement falling below the LOQ as less than the established quantification limit or expressing a non-detect result as less than the detection limit. (epa.gov)
Express1
Blood1
  • On the other hand, CTCs are very rare, often present as only a few cells per milliliter of blood, which makes their detection challenging. (wikipedia.org)
Potential1
Early1
  • In addition, ASCO also recommends mammography for the early detection of relapse in breast cancer ( 5 ). (iiarjournals.org)
Platform1
  • Multiplexed digital transcript detection data was generated using the Nanostring nCounter platform and evaluated through a dedicated bioinformatics pipeline. (biomedcentral.com)
Review1
  • Herein, we review the history and the current status of CTC detection, discuss the prognostic, predictive, and diagnostic capabilities of these technologies, and describe application of CTCs as models for the study of tumor biology. (amegroups.org)