ELISAAntibodiesImmunosorbentSerological assaysMultiplexReagentsAntigenMolecularAntibodyImmunoassayInfectionMass spectrometryMethodsSecretionsCharacterizationCorrelateEndpointsProteinLigandSerumMethodologyToxinsAnalysesExclusion2001TestsReceptorResultsImmune responsesTissueImmunologyKitsTechniquesSuccessfullyConcentrationsAnalysisIncludeTechnologiesCell cultureDiseaseHighSpecificAnimalsDevelopmentStudyPotentialSensitive
ELISA10
- This research gives an replace on the kinetics of humoral immune response to extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) an infection and efficiency traits of serological protocols (lateral stream assay [LFA], chemiluminescence immunoassay [CLIA] and ELISA) used for evaluations of current and previous SARS-CoV-2 an infection. (iddx.info)
- 4. Immunological assays (ELISA, ELISPOT). (helsinki.fi)
- Methods: The effects of dust extracts on inflammatory gene expression were analyzed by quantitative polymerase chain reaction (qPCR), enzyme linked immunosorbent (ELISA) and western blot assays. (cdc.gov)
- Enzyme-linked immunosorbent assays (ELISA) have been developed for detection of several SEs. (usda.gov)
- In addition to more and more antibodies , antigens and assays for SARS-CoV-2 research, we have added thousands of new recombinant antibodies and other proteins, conjugated secondary antibodies, protease inhibitors, ELISA kits and contract custom services. (linscottsdirectory.com)
- After 28 days of booster vaccinations, immunological responses among the animals were measured using enzyme-linked immunosorbent assays (ELISA). (news-medical.net)
- Compared to ELISA and other immunological detection methods, mass spectrometry (MS) is simpler in terms of sample preparation and method development. (shimadzu.com)
- ELISA IgG-avidity when used in combination with ELISA IgG/IgM is a valuable assay for the exclusion of ongoing or recently acquired T. gondii infection in pregnant women. (who.int)
- La présente étude menée dans la partie orientale de l'Iraq a examiné le test ELISA d'avidité des anticorps IgG pour dépister les infections à Toxoplasma gondii récentes chez les femmes enceintes, puis a comparé les méthodes immunologiques et la méthode PCR en tant que dosages moléculaires pour la pose du diagnostic de l'infection à T. gondii . (who.int)
- PCR assay is an important plasma kits (ELISA Toxo-IgG and IgM, plications in pregnant women and technique to evaluate the prevalence IBL International). (who.int)
Antibodies5
- The Search Engine for Research Antibodies, Antigens, Assays and More. (linscottsdirectory.com)
- Along with these very topical virus-related products, our suppliers are also adding thousands of other brand new antibodies, antigens, assays, custom services and more every week. (linscottsdirectory.com)
- Earlier studies demonstrated the advantages of immunofluorescence assays, based on polyclonal antibodies to B. anthracis cell-surface antigens, for identifying B. anthracis isolates ( 8 ) and directly evaluating clinical specimens from infected guinea pigs ( 9 ). (cdc.gov)
- But, in all sandwich assays, the signal begins to plateau with high concentrations of analyte due to limiting amounts of reagent antibodies and rare samples with extremely high concentrations of analyte can even lead to the hook effect in assays with a wash step. (lww.com)
- Our results demonstrate a high sensitivity of both assays and show that opsonising antibodies possess a broad cross-reactivity against various FMDV isolates within one serotype and in some cases even across serotypes. (forschung3r.ch)
Immunosorbent4
- 13. Garber E. A. E., Walker J. L., O'BRIEN T. W. Detection of abrin in food using enzyme-linked immunosorbent assay and electrochemiluminescence technologies // Journal of food protection. (medline.ru)
- 19. Leith A. G., Griffiths G. D., Green M. A. Quantification of ricin toxin using a highly sensitive avidin/biotin enzyme-linked immunosorbent assay //Journal of the Forensic Science Society. (medline.ru)
- 25 ] In India, the laboratory methods commonly followed are immunometric and enzyme-linked immunosorbent assays where the prevalence of hook effect is more. (lww.com)
- Techniques for measuring protein S antigen include enzyme-linked immunosorbent assays (ELISAs) and radioimmunoassays. (medscape.com)
Serological assays3
- Given the numerous efficiency traits of all of the serological assays, there's a must repeatedly enhance their detection thresholds, in addition to to watch and re-evaluate their performances to guarantee their significance and applicability for COVID-19 scientific and epidemiological functions. (iddx.info)
- High-dose effect is a common phenomenon documented in various immunological and serological assays, which results in false-negative results. (lww.com)
- More than 60 years ago, the phenomenon of high-dose hook effect had been documented in serological assays, which resulted in false-negative results. (lww.com)
Multiplex1
- Gene expression and cytokine/chemokine secretion were assessed by real-time PCR and multiplex assay, respectively. (karger.com)
Reagents2
- To that end, the Institute will support developing new assays and reagents to characterize immune responses as new vaccine platforms and antigenic targets evolve. (nih.gov)
- Be sure to include an intellectual property strategy to address any issues with your use of assays or reagents for research purposes or commercial development. (nih.gov)
Antigen2
- 2. Interferon γ cytokine secretion was determined by ELISPOT to assess PBMC response against known antigens in a preliminary experiment to approximate usable range for the following antigen specific PBMC assays. (helsinki.fi)
- In this study, we have evaluated and validated a two-component direct fluorescent-antibody (DFA) assay, using the monoclonal immunoglobulin (Ig) M antibody EAII-6G6-2-3 against the cell wall polysaccharide antigen (CW) ( 12 ) and the monoclonal IgG antibody FDF-1B9 against the capsule antigen (CAP) ( 16 ) for rapid identification of B. anthracis . (cdc.gov)
Molecular2
- This article describes using a liquid chromatograph/mass spectrometer to determine molecular mass and construct a quantitative assay for a therapeutic oligonucleotide and its analogs. (shimadzu.com)
- Girolami A, Vettore S, Scarparo P, Lombardi AM. Persistent validity of a classification of congenital factor X defects based on clotting, chromogenic and immunological assays even in the molecular biology era. (medlineplus.gov)
Antibody6
- In addition, mice sera were assessed by ADCC (antibody-dependent cellular cytotoxicity) reporter assays. (news-medical.net)
- Mice were euthanized after three days and five days of SARS-CoV-2 to obtain their pulmonary tissues for viral load quantification by classic plaque assays, and their antibody titers were determined. (news-medical.net)
- Through this integration with the FMD-DISCONVAC project, we will be fulfilling the objectives of the EU call KBBE-2008-1-3-02: To substitute vaccine potency tests in animals by assays enabling a correlation of in-vitro tests based on immunological principles of antibody-based effector immune responses operating in vivo. (forschung3r.ch)
- Based on immunological evidence obtained from previous work, we are developing in-vitro screening assays based on the relationship between protection and the avidity of specific antibody for binding to the pathogen in question. (forschung3r.ch)
- Immunological background: FcR-mediated anti-FMDV responses induced in plasmacytoid dendritic cells (pDC) and macrophages (MF) as examples of antibody-mediated effector functions independent of direct virus neutralization. (forschung3r.ch)
- Neutralization antibody titre was determined using microneutralization assays. (bvsalud.org)
Immunoassay1
- Kinetic exclusion assay have also been applied for concentration immunoassay, where it has proven capable of providing the maximum theoretical, Kd limited, sensitivity. (wikipedia.org)
Infection3
- Our study aimed to assess the overall immunological consequences of ex vivo infection of PBLs with VSV. (karger.com)
- Two types of immunological methods are used for detecting Mycobacterium tuberculosis infection: tuberculin skin tests (TSTs) and interferon-gamma release assay (IGRA) blood tests. (cdc.gov)
- These include an assay measuring enhanced infection of bovine monocyte-derived dendritic cells by immune complexed-FMDV and for the porcine system enhanced IFN-alpha responses by plasmacytoid DC. (forschung3r.ch)
Mass spectrometry1
- and high-throughput assays, such as next-generation sequencing, transcriptomics, mass spectrometry and proteomic analysis. (mayo.edu)
Methods3
- Because the fluorescent secondary molecule is applied after capture of the free receptor from solution (Figure 2) the binding constants measured using a kinetic exclusion assay are for unmodified molecules in solution and thus more accurately reflects endogenous binding interactions than methods requiring modification (typically labeling or immobilization) before measurement. (wikipedia.org)
- However, these immunological methods cannot distinguish between active and inactive toxin. (usda.gov)
- Journal of Immunological Methods. (lu.se)
Secretions2
- The identification of immunological markers in nasal secretions and tears is becoming essential in the study of allergic diseases. (medscape.com)
- Secretions reflect the local inflammatory activity and provide valuable information about the immunological reaction to allergens at the target organ. (medscape.com)
Characterization1
- Clinical, immunological and virological characterization of COVID-19 patients that test re-positive for SARS-CoV-2 by RT-PCR. (bvsalud.org)
Correlate2
- Applicants should already have access to well-characterized human clinical samples to correlate assay results to known disease or vaccination. (nih.gov)
- Since the investigation of vaccine dosing protocol in RCT is limited by feasibility issues, in-silico modeling can make an important contribution to the field, allowing the extensive exploration of different schedules and the identification of the immunological variables that correlate with the endpoints of interest ( 5 , 6 ). (frontiersin.org)
Endpoints2
Protein6
- Protein S assays can be either functional or immunological. (medscape.com)
- For detecting free protein S, the C4bBP-bound protein S is removed from the assay by a pre-assay step involving precipitation of the C4bBP-bound protein S with polyethylene glycol and then using centrifugation to remove the precipitate. (medscape.com)
- Functional assays measure only free (physiologically active) protein S. Functional assays are usually based on either the prothrombin time (PT) or the activated partial thromboplastin time (aPTT). (medscape.com)
- With aPTT-based functional protein S assays, platelet-poor plasma is incubated at 37°C with the following: protein S-deficient plasma, phospholipid, a contact activator (eg, Kaolin), and either an excess of activated protein C or an activator of protein C .Typically, the incubation period is 1-4 minutes, after which calcium is added to initiate clotting. (medscape.com)
- For PT-based functional protein S assays, the activated protein C cofactor activity of protein S is measured based on the degree of prolongation of a PT-based clotting time. (medscape.com)
- Immunological assays measure total protein S, both free protein S and bound protein S. In rare circumstances, the level of immunological protein S is normal but some functional abnormality exists (ie, a true type 2 deficiency). (medscape.com)
Ligand2
- A kinetic exclusion assay (KinExA) is a type of bioassay in which a solution containing receptor, ligand, and receptor-ligand complex is briefly exposed to additional ligand immobilized on a solid phase. (wikipedia.org)
- During the assay, a fraction of the free receptor is captured by the solid phase ligand and subsequently labeled with a fluorescent secondary molecule (Figure 1). (wikipedia.org)
Serum1
- Kinetic exclusion assays have been performed using unpurified molecules, in serum, and have measured binding to cell membrane proteins on intact whole cell which brings the measured binding interactions closer to their endogenous state. (wikipedia.org)
Methodology1
- Given that phosphorothioate modifica-tion is the most common method of enhancing oligonucleotide stability in vivo, we expect MRM-based assays constructed by a similar methodology to be applicable across a variety of therapeutic oligonucleotides. (shimadzu.com)
Toxins1
- Tests that detected GDH, toxins A/B, or toxin genes were included, and all sites underwent a training period provided by assay manufacturers. (mlo-online.com)
Analyses2
- With biochemical and immunological-based analyses, the identification and enumeration of specific microbial contaminants in environmental samples has been improved. (conceptcontrols.com)
- Immunological analyses confirmed the heterodimerization of A2 and B5 in transfected cells and their colocalization in the anterior pituitary. (jci.org)
Exclusion1
- Kinetic exclusion assays have been used to measure Kd's in the nanomolar to femtomolar range. (wikipedia.org)
20012
- Both DFA assays tested positive on 14 of 26 clinical specimens from the 2001 anthrax outbreak investigation. (cdc.gov)
- In addition to use on isolates, this rapid DFA assay was applied successfully to detect B. anthracis directly in clinical specimens from several patients with laboratory-confirmed inhalational anthrax during the 2001 bioterrorism-associated anthrax outbreak in the United States ( 6 , 17 ). (cdc.gov)
Tests2
- These assays were combined with immunological toxin tests. (mlo-online.com)
- This does not necessarily imply that in all instances the LLNA should be used in place of guinea pig tests, but rather that the assay is of equal merit and may be employed as an alternative in which positive and negative results generally no longer require further confirmation. (cdc.gov)
Receptor1
- Recombinant A2/B5 heterodimeric glycoproteins, purified using cation exchange and size fractionation chromatography, activated human TSH receptors, but not LH and FSH receptors, and showed high affinity to TSH receptors in a radioligand receptor assay. (jci.org)
Results2
- Although several standard microbiologic assays are available to identify B. anthracis ( 7 ) , they primarily lack timeliness in producing results. (cdc.gov)
- Reflex testing with a toxin assay as the second portion of the algorithm reduced the number of patients with discordant results to a more manageable number. (mlo-online.com)
Immune responses1
- NIAID seeks improved sampling and assays for assessing mucosal immune responses. (nih.gov)
Tissue1
- All assays were performed at the individual sites, and testing consisted of toxigenic culture and tissue culture assay. (mlo-online.com)
Immunology1
- Apply through our new funding opportunity announcement (FOA) Development and Optimization of Next-Generation Immunological Assays To Support Influenza Clinical Studies and Trials (UH2/UH3, Clinical Trial Not Allowed) if you can build a well-integrated, cross-disciplinary research team with diverse expertise in assay development and optimization, clinical research, immunology, and influenza virology. (nih.gov)
Kits1
- Manufacturer of immunological assay kits. (itk.nl)
Techniques1
- High-dose hook effect still remains a problem in many assay techniques like one-step immunometric assays, [ 2 3 4 5 6 7 8 9 ] immunoturbidimetric assays, [ 10 ] and immunonephelometric assays. (lww.com)
Successfully1
- We offer extensive expertise in many technologies needed to successfully commercialize medical devices and IVD assays. (sigmaaldrich.com)
Concentrations1
- Nasal washings are associated with an unpredictable, high dilution and concentrations of markers often fall below detection limits of immunological assays. (medscape.com)
Analysis1
- 5. Immunological bioinformatics analysis tools (IEDB) and database search (UniPROT). (helsinki.fi)
Include1
- Your application should include plans for two years of support in the UH2 phase, in which you'll pursue early development of an immunological assay, and three years of support in the UH3 phase, in which you'll further develop and optimize the assay. (nih.gov)
Technologies2
- ASSAY and Drug Development Technologies. (wikipedia.org)
- Advancing Assay & Test Technologies. (itk.nl)
Cell culture1
- Vero E6 cells were used for cell culture experiments, and neutralization assays were performed. (news-medical.net)
Disease1
- Articles were examined, specifically the ones dealing with information regarding genetic and/or immunological studies of individuals related to their disease susceptibility. (bvsalud.org)
High2
- Immunological toxin positives were not seen to have a high enough sensitivity to be the primary screen, but when positive after testing with GDH or NAAT, were considered confirmatory for C. difficile . (mlo-online.com)
- High Accuracy from Immunochromatographic Assays by the Use of Internal Control Ratios // Clinical Chemistry. (medline.ru)
Specific1
- The two-component DFA assay is a sensitive, specific, and rapid confirmatory test for B. anthracis in cultures and may be useful directly on clinical specimens. (cdc.gov)
Animals1
- Seasonal CoV S induced robust and targeted immunological responses among the animals with considerable alpha-CoV and beta-CoV cross-reactivity. (news-medical.net)
Development1
- You'll also need to use appropriate positive and negative controls for assay development. (nih.gov)
Study2
- The relationship of depression and stressors to immunological assays: a meta-analytic study. (bvsalud.org)
- The purpose of this study was to review the literature in order to comprehend the genetic and immunological features of aggressive periodontitis. (bvsalud.org)
Potential1
- Using a yeast two-hybrid assay, the two subunits were found as potential heterodimerization partners. (jci.org)
Sensitive1
- To develop a quantitative method using LC-QTOF-MS and LC-TQ-MS, we selected a product ion derived from phosphorothioate as the MRM transition and then constructed a highly sensitive MRM-based quantitative assay. (shimadzu.com)