CD56DifferentiationStaining of tumor cellsMarkersAntibodyChromogranin and synaptophysinHistological and immunohistochemicalNeurofilamentStainsCytoplasmicUltrastructuralDiagnosisExaminationsGlialTumorsSynaptic vesicle proteinPostnatal dayIntermediate FilamentsHistopathologicNeuroendocrine cellsNeuronalExpressionsFocalPositiveParaffinNeoplastic cellsStudyNeuralPresentExpressionCaseUniqueResearch
CD565
- Immunohistochemical staining for CD56 and synaptophysin was positive ( Figure 2 ). (cancerdiagnosisprognosis.org)
- Recently, insulinoma-associated protein 1 (INSM1) has been identified as a novel neuroendocrine marker that is more sensitive than chromogranin, synaptophysin, and CD56 in pulmonary neuroendocrine tumors. (gastrores.org)
- We studied this marker in conjunction with chromogranin, synaptophysin, and CD56 in 36 appendiceal AdexGCCs (21 primaries, 15 metastatic). (gastrores.org)
- Primary and metastatic AdexGCCs showed no difference in INSM1, chromogranin, synaptophysin, or CD56 staining. (gastrores.org)
- Immunohistochemically, the tumor was positive for chromogranin A, CD56, and synaptophysin, and a diagnosis of paraganglioma of the urinary bladder was confirmed. (biomedcentral.com)
Differentiation5
- The tumors were screened for neural differentiation with an antibody panel consisting of neuron-specific enolase, S100 protein, Leu-7, chromogranin, synaptophysin, and neurofilament. (nih.gov)
- Hence, five of 11 SRCT of bone or soft tissue with the t(11;22) showed morphologic and/or immunohistochemical evidence of neural differentiation. (nih.gov)
- The clinical significance of additional cytogenetic abnormalities and of morphologic or immunohistochemical evidence of neural differentiation in this group of tumors needs to be further studied. (nih.gov)
- Breast carcinoma showing neuroendocrine differentiation: immunohistochemical staining for synaptophysin. (neuromics.com)
- Large cell neuroendocrine carcinoma (LCNEC) is now recognized as a histologically high grade non small cell carcinoma showing histopathological features of neuroendocrine differentiation as well as immunohistochemical neuroendocrine markers. (ersjournals.com)
Staining of tumor cells1
- Immunohistochemical staining of tumor cells showed positive expressions of prolactin, synaptophysin and chromogranin A in the cytoplasm of the tumor cells. (tcr-signal.com)
Markers8
- Available sections were immunostained for keratin (OSCAR and AE1/AE3 antibodies), desmin, neurofilament protein, glial fibrillary acidic protein, synaptophysin, and chromogranin A. Not all cases could be tested for all markers. (elsevierpure.com)
- Immunohistochemistry was performed in both tissue and 3D sections for markers including synaptophysin, vimentin, neurofilament and MIB-1. (frontiersin.org)
- Adenocarcinoma ex-goblet cell carcinoid (AdexGCC) was considered a neuroendocrine adenocarcinoma, despite majority of tumor cells being negative for conventional neuroendocrine markers such as chromogranin and synaptophysin. (gastrores.org)
- Conventional neuroendocrine markers, such as chromogranin and synaptophysin, however, are often negative or focally positive in these tumors [ 2 , 3 ]. (gastrores.org)
- We present an unusual case of sclerosing Sertoli cell tumor in a 33-year-old Caucasian male, which was negative or weakly reactive using immunohistochemical markers typically positive in Sertoli cell tumors. (qxmd.com)
- Our case highlights that expected immunohistochemical markers may be negative, and awareness of antigenically unreactive tumors is needed to avoid confusion between Sertoli cell tumor and other entities. (qxmd.com)
- Both types of tumor giant cells stained for vimentin, α-1-antitrypsin, α-1-antichymotrypsin, synaptophysin, muscle actin, and neuron-specific enolase, but not for epithelial markers. (elsevierpure.com)
- The neoplastic cells routinely express pan-cytokeratin and neuroendocrine markers chromogranin and synaptophysin (focal to diffuse staining). (pbpath.org)
Antibody2
- Customer Testimonial: "The synaptophysin antibody works very nice. (neuromics.com)
- Synaptophysin Antibody detects endogenous levels of total Synaptophysin. (affbiotech.com)
Chromogranin and synaptophysin3
- INSM1 exhibits low expression in AdexGCCs and is expressed by a lower proportion of tumor cells compared to chromogranin and synaptophysin. (gastrores.org)
- Hristov et al previously demonstrated that chromogranin and synaptophysin were only expressed in 37% and 30% of AdexGCCs, respectively [ 2 ]. (gastrores.org)
- In contrast, conventional carcinoids are often diffusely positive for chromogranin and synaptophysin, with abundant electron-dense granules on electron microscopy [ 4 ]. (gastrores.org)
Histological and immunohistochemical3
- The rare case of metastatic infiltration of the thyroid gland by a MCC based on histological and immunohistochemical findings was described. (springeropen.com)
- The larynges were excised for histological and immunohistochemical examinations at 1, 7, 14, 28, and 56 days after administration. (biomedcentral.com)
- After the detailed clinical, histological, and immunohistochemical study we revealed some ultrastructural characteristics of the tumor, as well as the first evidence of direct intercellular connection between the tumor cells via gap junctions. (360qikan.com)
Neurofilament1
- The immunohistochemical study was conducted with primary antibodies: Neurofilament, Myelin basic protein, Glial fibrillary acidic protein, and Synaptophysin. (360qikan.com)
Stains5
- All of the remaining immunohistochemical stains were negative. (nih.gov)
- Stains for keratin, synaptophysin, and chromogranin were negative. (elsevierpure.com)
- Immunohistochemical stains, including laminin and collagen type IV, can be employed to highlight interruption of the basal lamina and document the diffuse architecture seen on hematoxylin and eosin stained slides. (cap.org)
- Immunohistochemical stains were performed on the biopsy material. (pbpath.org)
- Given how unusual these histologic features are compared to a typical low grade neuroendocrine tumor, immunohistochemical stains are an essential adjunct aid. (pbpath.org)
Cytoplasmic1
- D-Detection of synaptophysin is evident with mild to moderate cytoplasmic staining (arrows) of a portion of the tumor cells. (avma.org)
Ultrastructural1
- The journal spotlights ongoing advances in the uses of electron microscopic and immunohistochemical techniques, correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, electron probe analysis, and important new investigative, clinical, and diagnostic EM methods. (360qikan.com)
Diagnosis2
- Awareness of this phenomenon, careful histopathological evaluation, and an appropriate melanocytic immunohistochemical panel should facilitate the diagnosis of malignant melanoma with unusual immunophenotypes. (elsevierpure.com)
- A definitive diagnosis requires specialized immunohistochemical examinations. (biomedcentral.com)
Examinations1
- Immunohistochemical examinations showed that the tumor cells were synaptophysin- and NeuN-positive but GFAP-negative. (biomedcentral.com)
Glial1
- Tissue blocks were cut into 4-μm slides, deparaffinized in xylene, rehydrated with graded alcohols, and immunostained with the following antibodies: cytokeratin (CK, AE1/AE3), glial fibrillary acidic protein (GFAP, GA-5), synaptophysin (SP11), NeuN (A60) and Ki67 (MIB-1) (MaiXin, China). (biomedcentral.com)
Tumors4
- It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses. (wikipedia.org)
- Malignant melanoma showing anomalous intermediate filament and synaptophysin expression may easily be mistaken for carcinomas, rhabdomyosarcomas, and neuroendocrine tumors. (elsevierpure.com)
- Clinical, cytogenetic, histopathologic, and immunohistochemical data were obtained in a series of 11 small round cell tumors (SRCT) of bone and soft tissue with the translocation t(11;22) (q24;q12). (nih.gov)
- Due to similar morphological features of PTPR with a number of other papillary-like tumors of the pineal region, immunohistochemical analysis is crucial to its distinction. (surgicalneurologyint.com)
Synaptic vesicle protein2
- Synaptophysin, also known as the major synaptic vesicle protein p38, is a protein that in humans is encoded by the SYP gene. (wikipedia.org)
- The exact function of the protein is unknown: it interacts with the essential synaptic vesicle protein synaptobrevin, but when the synaptophysin gene is experimentally inactivated in animals, they still develop and function normally. (wikipedia.org)
Postnatal day2
- The level of synaptophysin increases progressively from postnatal day 7 up to 3 weeks in the archi- and paleocerebellum, and up to 6 weeks in the neocerebellum. (northwestern.edu)
- Next, brains were collected to examine hippocampus via immunohistochemical and biochemical studies on postnatal day 35 (P35). (istinye.edu.tr)
Intermediate Filaments2
- We have seen in consultation a number of malignant melanomas with anomalous expression of keratin, other intermediate filaments, or synaptophysin, and therefore studied a large group of primary and metastatic melanomas to determine the frequency of these events. (elsevierpure.com)
- Anomalous expression of all intermediate filaments and synaptophysin was found in significant subsets of malignant melanoma, representing potentially serious diagnostic pitfalls. (elsevierpure.com)
Histopathologic1
- Postoperative histopathologic examination and immunohistochemical analysis were consistent with carcinoid tumor. (biomedcentral.com)
Neuroendocrine cells1
- Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroblastomas. (neuromics.com)
Neuronal2
- Immunohistochemical examination revealed neuronal tumor immunophenotype with diffuse positivity for non-specific enolase, synaptophysin, and focal positivity for S-100. (thieme-connect.com)
- Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. (neuromics.com)
Expressions2
- This study reveals the expressions of Alzheimer's disease-related amyloid precursor protein, presenilin-1, and a presynaptic marker protein, synaptophysin, in the archi-, paleo- and neocerebellum during the postnatal development of the rat. (northwestern.edu)
- It is concluded that the postnatal expressions of soluble and membrane-bound amyloid precursor protein, synaptophysin and presenilin-1 are regulated differently during the ontogenetical development of the archi-, paleo- and neocerebellum of rat. (northwestern.edu)
Focal1
- The neoplastic cells were positive for pan-CK (strong and diffuse), synaptophysin (strong and diffuse) [Figure 2], chromogranin (strong but focal) and inhibin (strong and diffuse) while negative for CK7, RCC, PAX-8, S-100, Melan-A (MART-1), and beta catenin. (pbpath.org)
Positive2
- The immunohistochemical staining of her tumour tissues was positive for Chromogranin A, Synaptophysin and ACTH. (uwi.edu)
- synaptophysin or chromogranin positive by immunohistochemical staining is insufficient to diagnose SCC). (trialstoday.org)
Paraffin1
- IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. (affbiotech.com)
Neoplastic cells1
- The multifunctional nuclear protein large T-antigen was detectable by immunohistochemical analyses in a subset of neoplastic cells. (cdc.gov)
Study1
- An Immunohistochemical study conducted in 2009 of 54 individuals diagnosed with PDCIS found that the tumor cells in 34, 31, and 35 cases, respectively, expressed the estrogen receptor, progesterone receptor, and HER2/neu protein. (wikipedia.org)
Neural1
- Using immunohistochemistry, synaptophysin can be demonstrated in a range of neural and neuroendocrine tissues, including cells of the adrenal medulla and pancreatic islets. (wikipedia.org)
Present1
- In a 3-week-old animal, synaptophysin staining is present in all areas of the cerebellar molecular layer and in the internal granule cell layer. (northwestern.edu)
Expression1
- C3G administration mitigated tau phosphorylation and improved synaptic function and plasticity by upregulating the expression of synapse-associated proteins synaptophysin and postsynaptic density protein-95. (biomedcentral.com)
Case1
- The extensive homologies in immunohistochemical staining of both osteoclastic and pleomorphic giant cells in this case indicates that these cells are histogenetically related. (elsevierpure.com)
Unique1
- This rare tumor had a unique immunohistochemical profile. (elsevierpure.com)
Research1
- Recent research has shown, however, that elimination of synaptophysin in mice creates behavioral changes such as increased exploratory behavior, impaired object novelty recognition, and reduced spatial learning. (wikipedia.org)