PCNAProliferationApoptosisExpressionCyclinAntibodiesMarkersProteinAntibodyLesionsNeoplasmsMorphologicQuiescentHistologicalDifferentiationDetectionHistochemicalEpithelial CellsKidneyTumorsAnalysesSituEndothelial cellTissuesStudyVitroExaminationColorectalMalignantProteinsCOLON-CANCERPathologicalMitoticGlomeruliHistopathologicalPathwaysAtypicalTumor cellsRegenerationBovineBiolEpitheliumAssociated to epithelialCancersSquamous cellStemVascularProgenitor
PCNA27
- This retrospective immunohistochemical study compares the expression of five stress-response (heat-shock) proteins (srp's) [srp 90, srp 72, srp 27, alpha B-crystallin and ubiquitin], p53 protein and proliferating cell nuclear antigen (PCNA) in 118 primary brain tumors and 21 carcinoma metastases to the central nervous system. (elsevierpure.com)
- A high proportion of p53-positive cells (31.3 to 59.0%) and the highest PCNA-LIs (41.0 to 49.0%) were seen in two GBMs and one Br-Mt that expressed all five srp's. (elsevierpure.com)
- Andrej Cör To improve our understanding of the basic biological mechanisms of Hürthle cell tumours (HCT), proliferative activity in 20 HCT was determined by Ag-NOR (nucleolar organiser regions) counting and by immunohistochemical markers proliferating cell nuclear antigen (PCNA) and MIB-1. (karger.com)
- PCNA is a 36-KD proliferating cell nuclear antigen associated with the cell cycle. (uchile.cl)
- Immunohistochemical (IHC) examination with antibodies against proliferating cell nuclear antigen (PCNA) and Ki-67 were detected in the basal layer of the epidermis and connective tissue. (ankara.edu.tr)
- Hatipoglu F, Ozdemir O, Kıran MM (2009): Detection of argyrophil nucleolar organizer regions (AgNORs) and proliferating cell nuclear antigen (PCNA) in epithelial skin tumours from domestic animals. (ankara.edu.tr)
- Karademir N, Güvenç T, Yarım M, Orman MM, Gülbahar MY (1996): Comparison of agnors staining and pcna immunostaing methods, and mitotic index scores in intracutaneous cornifying epithelioma and squamous cell carcinoma. (ankara.edu.tr)
- Kawahira K (1999): Immunohistochemical staining of proliferating cell nuclear antigen (PCNA) in malignant and nonmalignant skin diseases. (ankara.edu.tr)
- Lu S, Tiekso J, Hietanen S, Syrjaènen K, Havu VK, Syrjaènen S (1999): Expression of cell-cycle proteins p53, p21 (WAF-1), PCNA and Ki-67 in benign, premalignant and malignant skin lesions with implicated HPV involvement. (ankara.edu.tr)
- Proliferating cell nuclear antigen (PCNA) and Ki-67 are such kind of molecular markers which may have prognostic significance in RCC and need to be studied about. (ijsurgery.com)
- Estimation of proliferation index by immunohistochemical expression analysis of PCNA and Ki-67 at different clinical stages of RCC samples and correlations of expression of the genes with different clinicopathological parameters between tumour tissue cells and adjacent normal tissue cells were the objectives. (ijsurgery.com)
- whereas the clear cell RCC, papillary RCC and chromophobe RCC showed 49.81%, 50.75% and 66.50% of mean PCNA expression respectively. (ijsurgery.com)
- Schönenberger F, Deutzmann A, Ferrando-May E, Merhof D. Discrimination of cell cycle phases in PCNA-immunolabeled cells. (ijsurgery.com)
- Its effect on proliferating cell nuclear antigen (PCNA) expression in actively proliferating cells, type I collagen expression and tissue remodeling in the healing tissue, and the association of tissue-transglutaminase (t-TGase) with wound healing were investigated by immunohistochemical staining. (tmu.edu.tw)
- Percentages of glomeruli with crescents and these with more than 50 sclerotic region optimistic for PAS and periodic acid ethenamine silver had been calculated The expression of proliferating cell nuclear antigen (PCNA), phospho-STAT3, and fibrin/fibrinogen was evaluated by immunostaining. (calcimimeticagent.com)
- Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. (ihcworld.com)
- Synthesis of the nuclear protein cyclin (PCNA) and its relationship with DNA replication. (ihcworld.com)
- Proliferating celi nuclear antigen (PCNA) isapplicable to formalin-fixed, paraffin embedded tissues. (selcukmedj.org)
- İn this study, PCNA expression in cutaneous keratinous neoplasms were determined by immunohistochemical stain- ing using the 19A2 done. (selcukmedj.org)
- SCC and VV showed significantly increased numbers of PCNA positive cells when compared with other keratinocytic neoplasms. (selcukmedj.org)
- Most of the LC In the lymph nodes we examined were negative for the anti-proliferating nuclear antigen (PCNA) antibody. (fujita-hu.ac.jp)
- PCNA or TUNEL optimistic cells were quantified in 40 randomly picked high power fields of every tissue area. (trpvantagonists.com)
- Objective To investigate the effects of Mitoxantrone on the expression of proliferating cell nuclear antigen (PCNA), Caspase-3 and p53 genes in gastric mucosa of rats with gastric cancer. (shsmu.edu.cn)
- The expression of PCNA in tissues of gastric cancer was detected by immunohistochemical S-P method, the activity of Caspase-3 was determined by spectrophotometry, and the expression of p53 gene and protein was detected by Real-Time PCR and Western blotting, respectively. (shsmu.edu.cn)
- Immunohistochemical analysis of paraffin embedded Jurkat cell pellets, untreated (left) or etoposide-treated (right), using Phospho-Histone H3 (Ser10) Antibody, PCNA (PC10) Mouse mAb, Cleaved Caspase-3 (Asp175) Antibody and Survivin (71G4B7E) Rabbit mAb. (cellsignal.com)
- Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (6). (cellsignal.com)
- Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 7). (cellsignal.com)
Proliferation35
- The present study was to investigate the protection of resveratrol (RSV) in diabetes associated with kidney inflammation and cell proliferation. (hindawi.com)
- In vitro , RSV attenuated high glucose-induced plasminogen activator inhibitor (PAI-1) expression and mesangial cell proliferation, as well as Akt and nuclear factor-kappa B (NF- κ B) activation. (hindawi.com)
- These results suggest that RSV prevents diabetes-induced renal inflammation and mesangial cell proliferation possibly through Akt/NF- κ B pathway inhibition. (hindawi.com)
- Accumulated data suggest that the predicted evolution of diabetic glomerulopathy is comprised of an early, transient mesangial cell proliferation and subsequent hypertrophy of these cells that herald the slow progression into glomerulosclerosis [ 2 ]. (hindawi.com)
- demonstrated that RSV prevented high glucose-induced kidney mesangial cell proliferation and fibronectin expression through inhibition of high glucose-induced JNK and NF- B activation, NADPH oxidase activity elevation, and reactive oxygen species production [ 10 ]. (hindawi.com)
- In the present study, therefore, we aimed to determine whether RSV treatment attenuated renal inflammation and mesangial cell proliferation under diabetic condition both in vivo and in vitro . (hindawi.com)
- The knowledge of cell proliferative activity and its cellular proteins, which are involved in the control of cell proliferation, are essential components for understanding the biology of tumors. (thejcdp.com)
- With this view in mind, the present study is designed to elucidate the biological behavior of the cancer cells in OSCC respective to their location, using Ki-67 immunological probe for cellular proliferation. (thejcdp.com)
- The transplantation of iPSC-derived RPCs decreased the levels of biomarkers indicative of renal injury and attenuated the necrosis and apoptosis of renal tissues, but resulted in the up-regulation of renal tubules formation, cell proliferation, and the expression of pro-renal factors. (biomedcentral.com)
- Our results revealed that iPSC-derived RPCs can protect AKI rat from renal function impairment and severe tubular injury by up-regulating the renal tubules formation, promoting cell proliferation, reducing apoptosis, and regulating the microenvironment in the injured kidney. (biomedcentral.com)
- In response to acute injury, the adult kidney shows some level of regeneration characterized by the proliferation of the surviving cells and the replacement of the necrotic tubular cells with functional tubular epithelium [ 3 ]. (biomedcentral.com)
- Meanwhile, proliferation and cell cycle of vascular smooth muscle cells (VSMCs), which were co-cultured with NETs were assessed. (bvsalud.org)
- Brugal G (1995): Quantitative microscopy and tumour cell proliferation. (ankara.edu.tr)
- Cell proliferation and apoptosis were assessed by immunohistochemical detection of pro liferating cell nuclear antigen and cleaved Cas pase 3, respectively. (alkpathway.com)
- The N-terminal phosphorylation of RB by p38 bypasses its inactivation by CDKs and prevents proliferation in cancer cells. (nature.com)
- When an adequate number of cells (ASCs 10 × 10 6 and BM-MSCs 3 × 10 6 , because of their low rate of proliferation) was reached, two skin wounds were surgically induced in each animal. (biomedcentral.com)
- Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10. (ijsurgery.com)
- In: Chemically induced cell proliferation: Implications for risk assessment. (cdc.gov)
- Within this phase of NTN, the glomeruli turn into substantially hypercellular due to cell migration and cell proliferation (13). (calcimimeticagent.com)
- Effect of PRP on endometrial cell proliferation and on the expression of genes [prostaglandin-endoperoxide synthase 2 ( COX2 ), tumor protein p53 ( TP53 ), oestrogen receptors ( ER-α and ER-β ), progesterone receptor ( PR ) and c-Myc ] involved in the regulation of oestrus cycle and fetal-maternal interaction were evaluated. (biomedcentral.com)
- The mitotic index and apoptotic index were assessed by quantitative morphometric evaluation of proliferating cell nuclear antigen expression and in situ terminal transferase mediated fluorescein deoxy UTP nick finish labeling,two established markers of proliferation and apoptosis. (trpvantagonists.com)
- KLF4 plays roles in cell proliferation, differentiation and survival. (trpvantagonists.com)
- ICOS belongs to your CD28 and CTLA 4 cell surface receptor family members, and it plays an im portant purpose in cell cell signaling, immune responses, and regulation of cell proliferation. (trpvantagonists.com)
- CD28 is important for T cell proliferation and survival, selleckchem cytokine manufacturing, and T helper sort two advancement. (trpvantagonists.com)
- Silibinin dose-dependently decreased AOM-induced colonic cell proliferation, evidenced by proliferative cell nuclear antigen and cyclin D1 immunohistochemical staining, and induced apoptosis in these colon tissues, evidenced by terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling staining and cleaved poly(ADP-ribose) polymerase. (nih.gov)
- Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation. (frontiersin.org)
- During rapid mammary growth in the mouse, label retaining epithelial cells (LREC) appear to retain label by asymmetric distribution of DNA strands, as evidenced by a rapid proliferation index of the LREC ( Smith, 2005 ). (frontiersin.org)
- During periods of low mammary proliferation, quiescence of the stem cell population may account for retention of label. (frontiersin.org)
- While Ascl1a and Lin28a are required for Müller glia proliferation, Stat3 is necessary for the maximal number of Müller glia to proliferate during regeneration of the damaged zebrafish retina. (zfin.org)
- Among its different constituents, Astragalus polysaccharides have been most widely studied particularly for their immunopotentiating properties, such as stimulation of murine B cell proliferation and cytokine production ( 6 ). (spandidos-publications.com)
- Our results indicated that AST inhibits cell proliferation through phase-specific cell cycle arrest and caspase-dependent apoptosis. (spandidos-publications.com)
- The SignalStain ® Proliferation/Apoptosis IHC Sampler Kit from Cell Signaling Technology allows the researcher to examine paraffin-embedded tissues or cells with antibodies that will detect cellular apoptosis or proliferation. (cellsignal.com)
- In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
- H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)
- Functional loss of p16 may lead to uncontrolled cell proliferation 3,4 . (bvsalud.org)
Apoptosis10
- In contrast, cells IR for the apoptosis marker C.CASP3 were remarkable in epithelial cells at the tips of intestinal villi and in MALT, reflecting the dynamic nature of the latter mentioned structures. (biomedcentral.com)
- Other authors have shown attenuated germ cell apoptosis SB-742457 kinase inhibitor following antioxidant therapy. (hifsignaling.com)
- Germ cell apoptosis was inhibited which has a substantial attenuation in testis fat reduction compared to untreated cryptorchid rats. (hifsignaling.com)
- Zini et al concluded that nitric oxide may possibly possess a central position in germ cell apoptosis from the testis. (hifsignaling.com)
- Immunohistochemical analysis was employed to determine the temporal and spatial expression of Stat3 and Ascl1a proteins following rod and cone photoreceptor cell apoptosis. (zfin.org)
- Knockdown of Stat3 protein expression did not affect photoreceptor apoptosis, but significantly reduced, without abolishing, the number of proliferating Ascl1a-positive Müller glia. (zfin.org)
- Knockdown of Ascl1a protein also did not change the extent of photoreceptor apoptosis, but did yield significantly fewer Müller glia that reentered the cell cycle relative to the stat3 morphant and significantly decreased the number and intensity of Stat3 expressing Müller glia. (zfin.org)
- Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). (cellsignal.com)
- Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle through inhibiting apoptosis and promoting cell division (4,5). (cellsignal.com)
- The various markers that enable assessment of the progression of preneoplastic lesions to spindle cell carcinoma include the p16 protein, which halts the cell cycle and induces apoptosis by pRb-mediated phosphorylation of cyclin-dependent kinase 4 (CDK4). (bvsalud.org)
Expression10
- We conclude that primary and metastatic tumors of the brain produce one or more stress-related proteins, and that a variable proportion of the tumor cells have immunohistochemically-detectable p53, the expression of which may depend, at least in part, on the growth potential of a given tumor. (elsevierpure.com)
- Immunohistochemical examination revealed positive expression of cluster of differentiation 34(CD34), vimentin and CD99, and negative expression of epithelial membrane antigen, S100 and glial fibrillary acidic protein. (spandidos-publications.com)
- This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. (thermofisher.com)
- Proliferating cell nuclear antigen expression was lower in diethylnitrosamine treated animals compared to the control and capsaicin groups, pointing to a disruption of the proliferative activity of hepatocytes in the juvenile liver. (vfu.cz)
- Immunohistochemical expression of aromatase and estrogen, androgen and progesterone receptors in normal and neoplastic human meningeal cells. (journal-jmsr.net)
- Whereas the clear cell RCC, papillary RCC and chromophobe RCC showed 23.96%, 24.75% and 31% of mean Ki-67 expression respectively. (ijsurgery.com)
- Nuclear PKM2 interacts with HIF-1α and subsequently upregulates the expression of HIF-1α target genes encoding glycolytic enzymes, GLUT1, HK2, PKM2, LDHA and PDK1, which are required for the C. tropicalis -induced aerobic glycolysis of MDSCs. (biomedcentral.com)
- Finally, introduction of lin28a morpholinos resulted in decreased Müller glia expression of Stat3 and Ascl1a, significantly reducing the number of proliferating Müller glia. (zfin.org)
- Additionally, the expression and activity of matrix metalloproteinases (MMPs) are increased in almost every type of human cancer, which may be associated with cell invasion, metastasis and angiogenesis. (spandidos-publications.com)
- Expression of p63 is almost exclusively restricted to epithelial cells, mutations in this gene are infrequent, and its expression is increased in a variety of solid tumors, particularly those of the head and neck area 12,13 . (bvsalud.org)
Cyclin3
- The cell cycle is a tightly regulated process that is controlled by the conserved cyclin-dependent kinase (CDK)-cyclin protein complex 1 . (nature.com)
- Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. (ihcworld.com)
- Localization of the gene for human proliferating nuclear antigen/cyclin by in situ hybridization. (ihcworld.com)
Antibodies2
- Monoclonal antibodies directed toward nuclear antigens are increasingly used as tools to obtain valuable information concerning the proliferative changes of the tumors. (thejcdp.com)
- The antibodies and reagents used for immunohistochemical analysis were obtained from Leica Biosystems, Benton lane, Newcastle, UK. (thejcdp.com)
Markers7
- Next, we examined the renal function-related markers and renal histology to assess the therapeutic effect of the injected cells. (biomedcentral.com)
- Strategies for improving the immunohistochemical staining of various intranuclear prognostic markers in formalin-paraffin sections: Androgen receptor, estrogen receptor, progesterone receptor, p53 protein, proliferating cell nuclear antigen, and Ki-67 antigen revealed by antigen retrieval techniques. (journal-jmsr.net)
- Rabbit ASCs were isolated and expanded in vitro with relative abundance, cells expressed typical surface markers (CD49e, CD90 and CD29). (biomedcentral.com)
- Several molecular markers play important role in development and prognosis of renal cell carcinoma (RCC). (ijsurgery.com)
- Our results support the use of DNA label retention to identify MaSC and also provide a molecular profile and novel candidate markers for these cells. (frontiersin.org)
- Insights into the biology of stem cells will be gained by confirmation and characterization of candidate MaSC markers identified in this study. (frontiersin.org)
- Other markers, such as retinoblastoma and p53, may be related with early steps of carcinogenesis in oral cavity squamous cell carcinoma. (bvsalud.org)
Protein9
- Brain tumor: immunohistochemical studies on the stress-response proteins, p53 protein and proliferating cell nuclear antigen. (elsevierpure.com)
- Dive into the research topics of 'Brain tumor: immunohistochemical studies on the stress-response proteins, p53 protein and proliferating cell nuclear antigen. (elsevierpure.com)
- Two forms of this protein have been identified in animal cells, one in cytosol and one in mitochondria. (wikipedia.org)
- The Ki-67, a large nonhistone protein with molecular weight of approximately 395 kDa, has been extensively used as a marker to read the proliferative activity during the G1, S, G2, and M phases of the cell cycle. (thejcdp.com)
- Bullwinkel J, Baron-Lühr B, Lüdemann A, Wohlenberg C, Gerdes J, Scholzen T. Ki-67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells. (ijsurgery.com)
- Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (3). (cellsignal.com)
- DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)
- Conclusion: p63, p16, MIB, Cal A, Cys A are markedly expressed and p16 is strongly suppressed in oral cavity tumors, which suggests that the latter protein may play a role in negative regulation of cell cycle progression. (bvsalud.org)
- Another protein, calgranulin A (Cal A), is involved in the regulation of several cell processes, including the cell cycle and cell differentiation. (bvsalud.org)
Antibody4
- The sections were stained with an antibody directed against the Ki-67 antigen. (thejcdp.com)
- Immunohistochemical staining of Cdc20 in human testis and skeletal muscle tissues using Cdc20 Polyclonal Antibody (Product # PA5-63103). (thermofisher.com)
- The majority of the dendritic cells that existed in the LN of dermatopathic lymphadenopathy were positive for OKT-6 (CD 1a) antibody. (fujita-hu.ac.jp)
- Each antibody is validated for use in immunohistochemical assays using multiple approaches. (cellsignal.com)
Lesions4
- Do epidermal Langerhans cells, migrating from skin lesions, induce the paracortical hyperplasia of dermatopathic lymphadenopathy? (fujita-hu.ac.jp)
- This finding may mean that LC In the LN are fully developed cells and do not divide In the LN. Langerhans cells may migrate from the skin lesions to the paracortical areas in the LN, which then may become enlarged. (fujita-hu.ac.jp)
- Dive into the research topics of 'Do epidermal Langerhans cells, migrating from skin lesions, induce the paracortical hyperplasia of dermatopathic lymphadenopathy? (fujita-hu.ac.jp)
- Immunohistochemical identification of molecular genetic events in the progression of preneoplastic lesions to spindle cell squamous-cell carcinoma enables early detection of lesions with the potential for malignant progression, thus permitting timely intervention 1,2 . (bvsalud.org)
Neoplasms1
- Recent evidence suggests that the bicellular stem cell theory is the more probable etiology of salivary gland neoplasms. (medscape.com)
Morphologic3
- The evaluation of the morphologic profile of the tumor cells is considered to be a determinant factor appropriating the aggressiveness of the tumor. (thejcdp.com)
- In addition to this, morphologic profiles of the neoplastic cells are different and are unisomorphic at different zones in the same field as well as in different fields of the same tumor, thus creating a scope for confusion. (thejcdp.com)
- HGPIN is characterized by architecturally benign prostatic acini and ducts lined with atypical cells whose morphologic, histochemical, immunohistochemical, and genetic changes are similar to those of prostate cancer. (medscape.com)
Quiescent2
- The process is hypothesized to involve epithelial cell dedifferentiation, interstitial cell transdifferentiation and/or activation of quiescent stem cells. (biomedcentral.com)
- Thus, there are three populations of Müller glia in the light-damaged zebrafish retina: 1) Stat3-expressing Ascl1a-nonexpressing nonproliferating (quiescent) Müller glia, 2) Stat3-dependent Ascl1a-dependent proliferating Müller glia, 3) Stat3-independent Ascl1a-dependent proliferating Müller glia. (zfin.org)
Histological3
- We have therefore investigated whether the fractal dimension of nuclear chromatin measured in routine histological preparations of malignant melanomas could be a prognostic factor for survival. (biomedcentral.com)
- These approaches result in the loss of all histological information pertaining to the in vivo locale of MaSC and progenitor cells. (frontiersin.org)
- Methods: fifteen histological specimens of spindle cell squamous cell carcinoma of the lower lip were obtained from the Department of Oral Pathology, Bahia Federal University. (bvsalud.org)
Differentiation10
- [ 18 ] The more typical sebaceous tumors in MTS are sebaceous adenoma , sebaceoma, and basal cell carcinoma with sebaceous differentiation. (medscape.com)
- The tumor consists of irregular lobules and sheets of cells, as seen in the first three images below, with varying degrees of sebaceous differentiation, including scalloped, centrally located nuclei with foamy, multivacuolated cytoplasm, secondary to intracytoplasmic lipid (see the 4th and 5th images). (medscape.com)
- In this study, we induced iPSCs differentiation into RPCs using a nephrogenic cocktail of factors combined with the renal epithelial cell growth medium. (biomedcentral.com)
- We showed that the differentiation efficiency of iPSCs to RPCs increased when cultured with renal epithelial cell growth medium after stimulation with a nephrogenic cocktail of factors. (biomedcentral.com)
- Stem cells, including embryonic stem cells (ESCs) and adult stem cells, have the potential of self-renewal and differentiation into multiple cell types and therefore hold great promise in regenerative medicine [ 11 - 13 ]. (biomedcentral.com)
- Induced pluripotent stem cells (iPSCs) are a type of newly defined stem cell with properties similar to those of ESCs in terms of self-renewal and differentiation. (biomedcentral.com)
- These multipotent cells with innate self-renewal capacity can be in vitro expanded without losing their differentiation potential. (biomedcentral.com)
- whereas LREC in suprabasal epithelial layers are enriched for more committed progenitor cells, expressing some genes that are associated with stem cell attributes along with those indicative of cell differentiation. (frontiersin.org)
- This dictates cycles of mammary growth, differentiation, lactation, and regression, during which mammary stem cells (MaSC) provide for the lineages of luminal and basal (myoepithelial) epithelial cells in the ducts and alveoli. (frontiersin.org)
- Cystatin A (Cys A), a cysteine protease inhibitor, is a precursor of proteins involves in keratinocyte keratinization, and is expressed during the late phase of differentiation of these cells. (bvsalud.org)
Detection2
- The liver tissue was sampled 1, 2, and 4 days after surgery, and immunohistochemical detection of proliferating cell nuclear antigen TNF-α and IL-10 was performed. (geneticsmr.com)
- Slides of both organs stained with haematoxylin and eosin were histologically evaluated and immunohistochemical detection of proliferating cell nuclear antigen and glutamine synthetase in the liver tissue was performed. (vfu.cz)
Histochemical1
- In this study, anatomical, morphometrical, histochemical, and immunohistochemical techniques were employed to study the structure of the gastrointestinal tract of Gallinula chloropus . (biomedcentral.com)
Epithelial Cells1
- We isolated four categories of cells from mammary epithelium of female calves: bromodeoxyuridine label retaining epithelial cells (LREC) from basal (LRECb) and embedded layers (LRECe), and epithelial control cells from basal and embedded layers. (frontiersin.org)
Kidney3
- Cell-based therapy with mesenchymal stem cells or renal progenitor cells (RPCs) resident in the kidney has appeared to be an effective strategy for the treatment of AKI. (biomedcentral.com)
- However, progenitor cells in the adult kidney are rare, which limits the application of these cells. (biomedcentral.com)
- The remaining kidney half was snap-frozen and used for immunohistochemical study. (calcimimeticagent.com)
Tumors5
- Most astrocytomas (9/13), ependymomas (5/5), glioblastoma multiforme (GBM) (11/12), schwannomas (19/21), meningiomas (22/23) and breast carcinoma metastases (Br-Mt) (9/10), and some medulloblastomas (5/15), primitive neuroectodermal tumors (PNETs) (5/11), pituitary adenomas (4/7) and lung carcinoma metastases (6/11), but none of 10 oligodendrogliomas had tumor cells that expressed one or more (up to five) srp's. (elsevierpure.com)
- [ 2 , 3 ] This tumor is more common in Asian countries, reportedly comprising 33% of eyelid tumors and second behind basal cell carcinoma. (medscape.com)
- Hence, Ki-67 antigen-labeled cells can prove to be an effective tool to grade the tumors and finally contribute to the prognosis of the disease. (thejcdp.com)
- Mice had been sacrificed immediately after 28 days of solutions and tumors have been removed and fixed in 10% neutral buf fered formalin for histologic and immunohistochemical analyses. (trpvantagonists.com)
- This theory holds that tumors arise from 1 of 2 undifferentiated stem cells: the excretory duct reserve cell or the intercalated duct reserve cell. (medscape.com)
Analyses2
- Analyses of microvascular changes in dcSSc have focussed almost solely on the contribution of endothelial cells, largely overlooking the potential role of pericytes. (biomedcentral.com)
- Immunohistochemical analyses were performed at the Molecular Biology Laboratory of the Department of Otorhinolaryngology, Heidelberg University, Germany. (bvsalud.org)
Situ1
- Instead, we used laser microdissection to excise putative progenitor cells and control cells from their in situ locations in cryosections and characterized the molecular properties of these cells. (frontiersin.org)
Endothelial cell3
- Given their central role in modulating endothelial cell function, it is clear that the pronounced changes observed in endothelial cells during dcSSc will also alter pericyte phenotype and function. (biomedcentral.com)
- Its part, primarily during the context of cancer, is extensively studied, and various research have explored the function of KLF4 in vascular smooth muscle cell and vas cular endothelial cell. (trpvantagonists.com)
- The malformations have a normal endothelial cell growth cycle that affects the veins, the capillaries, or the lymphatics, and they do not involute. (medscape.com)
Tissues4
- IR cells for the mitosis marker phospho-histone H3 (PHH3) were highest within the entire intestinal crypts and mucosa-associated lymphoid tissues (MALT). (biomedcentral.com)
- Mesenchymal stromal cells were in vitro expanded from adipose and BM tissues of young female New Zealand rabbits. (biomedcentral.com)
- Furthermore, silibinin significantly decreased AOM-induced inducible nitric oxide synthase- and cyclooxygenase-2-positive cells in colon tissues. (nih.gov)
- The tumor size and the number of proliferating cells were reduced in the tumor tissues obtained from the formononetin-treated group. (spandidos-publications.com)
Study8
- The aim of the present study was to describe the clinical, histopathological and immunohistochemical aspects of naturally occurring bovine cutaneous papillomatosis.A total of 82 Holstein cattle (9.5%), aged between 5 and 24 months, were diagnosed as cutaneous papilloma by clinical examination. (ankara.edu.tr)
- There is a need to further stratify prognostically distinct groups of colorectal cancer, and the purpose of this study was to identify prognostically significant immunohistochemical marker profiles in colorectal cancer. (elsevierpure.com)
- Conclusions: This study has identified an immunohistochemical marker profile of colorectal cancer and showed that it is an independent indicator of prognosis in this type of cancer. (elsevierpure.com)
- The aim of this pre-clinical study was to investigate the effects of intradermal injections of MSC in experimental cutaneous wound repair comparing allogeneic and autologous adipose stem cells (ASCs) and autologous bone marrow-mesenchymal stromal cells (BM-MSCs). (biomedcentral.com)
- In the present study, immunohistochemical and immunoelectron microscopic techniques were used to differentiate Langerhans cells (LC) from interdigitating cells (IDC) in the lymph nodes (LN) of dermatopathic lymphadenopathy. (fujita-hu.ac.jp)
- Our previous study has revealed that Candida tropicalis ( C. tropicalis ) promotes colorectal tumorigenesis by enhancing immunosuppressive function of myeloid-derived suppressor cells (MDSCs) and increasing accumulation of MDSCs, but the underlying mechanisms remain unestablished. (biomedcentral.com)
- In the present study, we aimed to further examine the potential of formononetin in controlling angiogenesis and tumor cell invasiveness in human colon cancer cells and tumor xenografts. (spandidos-publications.com)
- Subsequent study using multiple human cancer cell lines, including those of the colon, stomach and liver, further indicated the universal growth-inhibitory and pro-apoptotic effects of AST. (spandidos-publications.com)
Vitro5
- Function of RSV has been extensively explored for its powerful antioxidant capacity and specific effects on proteins and/or signaling cascades, such as Sirt1, adenosine monophosphate activated kinase, phosphatidylinositol-3 kinase (PI3K)/Akt, and JNK/nuclear factor-kappa B (NF- B) in DN both in vivo and in vitro [ 8 - 10 ]. (hindawi.com)
- Mesenchymal stromal cells (MSCs) expanded in vitro have been proposed as a potential therapy for congenital or acquired skin defects in pediatrics. (biomedcentral.com)
- PRP was tested in vivo , after bovine intrauterine administration, and in vitro on endometrial cells. (biomedcentral.com)
- Moreover, to evaluate the ability of PRP to counteract inflammation, 10 and 100 ng/ml of bacterial endotoxin lipopolysaccharide (LPS) were used to inflame endometrial cells in vitro for 1, 6, 12, 24 and 48 h. (biomedcentral.com)
- Previous molecular characterizations of mammary stem cells (MaSC) have utilized fluorescence-activated cell sorting or in vitro cultivation of cells from enzymatically dissociated tissue to enrich for MaSC. (frontiersin.org)
Examination1
- Immunohistochemical examination of this tumor revealed positive staining for Cytokeratin 20, Mucin 2, and CDX2, although Cytokeratin 7 and Mucin 6 were negative. (hindawi.com)
Colorectal3
- Based on our recent silibinin efficacy studies in human colorectal cancer cells, we investigated the effects of its dietary feeding on azoxymethane (AOM)-induced aberrant crypt foci (ACF) formation and associated biomarkers in male Fisher 344 rats. (nih.gov)
- Blockade of PKM2 nuclear translocation attenuates C. tropicalis -mediated colorectal tumorigenesis. (biomedcentral.com)
- Another important mechanism for the progression of colorectal cancer is the ability of the transformed cells to invade and traverse the basement membrane, which in turn facilitates the conversion of adenomas into carcinomas and then metastases ( 1 ). (spandidos-publications.com)
Malignant1
- So, there is strong evidence that the fractal dimension of the nuclear chromatin texture is a new and promising variable in prognostic models of malignant melanomas. (biomedcentral.com)
Proteins1
- In particular, neoplastic growth induces important modifications, not only of the DNA, but also of the composition and distribution of the histone and non-histone nuclear proteins, thus provoking alterations of the distribution of heterochromatin in the nucleus. (biomedcentral.com)
COLON-CANCER4
- And more recently, we discovered that C. tropicalis promoted colitis-associated colon cancer (CAC) through enhancing the accumulation and immunosuppressive activity of myeloid-derived suppressor cells (MDSCs) [ 10 ]. (biomedcentral.com)
- Our previous findings demonstrated that formononetin initiates growth-inhibitory and pro-apoptotic activities in human colon cancer cells. (spandidos-publications.com)
- We also discovered that the invasiveness of metastatic colon cancer cells was alleviated following drug treatment. (spandidos-publications.com)
- In recent years, we focused on investigating the anti-carcinogenic properties of total Astragalus saponins (AST) in human colon cancer cells and tumor xenografts. (spandidos-publications.com)
Pathological1
- OBJECTIVE: Neutrophil extracellular traps (NETs) can trigger pathological changes in vascular cells or vessel wall components, which are vascular pathological changes of hypertension. (bvsalud.org)
Mitotic4
- Sebaceous carcinoma commonly shows marked nuclear atypia and mitotic figures (200× magnification). (medscape.com)
- Tumor thickness, Clark's level, mitotic rate, nuclear area and fractal dimension were significant risk factors in univariate Cox regressions. (biomedcentral.com)
- The tumor had a large proportion of mitotic cells and its resemblance to adenocarcinoma of the large intestine was striking. (hindawi.com)
- The high proportion of mitotic cells and resemblance to well-differentiated adenocarcinoma of the large intestine suggested that this neoplasm was adenocarcinoma rather than adenoma. (hindawi.com)
Glomeruli1
- Additionally, immunohistochemistry results indicated that RSV treatment reduced the density of proliferating cell nuclear antigen-positive cells significantly in glomeruli of diabetic mice. (hindawi.com)
Histopathological1
- 1 In addition to this, the studying of the histopathological profiles of the cancer cell has largely remained subjective, embodying a possibility of individual bias toward mendacious interpretation of the disease. (thejcdp.com)
Pathways1
- A p120ctn is really a regulator from the kaiso perform and it is identified that while in the nucleus of the cell they straight modulate the action of canonical Wnt pathways and target genes of B catenin, which can be another indication in the importance of Kaiso while in the growth of cancer. (alkpathway.com)
Atypical1
- Irregular lobules and sheets of atypical sebaceous cells (20× magnification). (medscape.com)
Tumor cells2
- No invasion or no microinvasion of the tumor cells was seen. (hindawi.com)
- c) Tumor cells show high-grade nuclear atypia. (hindawi.com)
Regeneration1
- Recent advances in stem cell therapy for tissue engineering approaches have provided promising data on wound repair and tissue regeneration. (biomedcentral.com)
Bovine2
- Bovine papillomavirus (BPV-1) antigens were detected in the basal layer. (ankara.edu.tr)
- Bovine endometrial cells were cultured until passage (P) 10 with 5 % or 10 % PRP. (biomedcentral.com)
Biol2
- J Cell Biol. (ijsurgery.com)
- Molecular Cell Biol. (ijsurgery.com)
Epithelium3
- They were mainly localized in the acini of the esophageal glands and SPG, gastric surface epithelium, duct system of DPG, and intestinal goblet cells. (biomedcentral.com)
- The two main sources of mucins within the digestive tract are the glandular acini located within the deep face of the mucosa and goblet cells dispersed throughout the gut epithelium [ 12 ]. (biomedcentral.com)
- 11] Mechanisms involving promotion of mast cell migration and activation in this disease are unclear, but recent research found an overexpression of stem cell factor (SCF) and thymic stromal lymphopoietin (TSLP) in the conjunctival epithelium of patients with SLK. (medscape.com)
Associated to epithelial1
- The endometritis is characterized by an increase number of inflammatory cells associated to epithelial erosion and/or necrosis and diffuse oedema of endometrium [ 1 ]. (biomedcentral.com)
Cancers2
- Cell kinetic data may also be a useful adjunct to histologically based tumor classifications, and are among the most important indicators of treatment response and relapse in many types of cancers. (thejcdp.com)
- The Ki-67 antigen is one such reliable biomarker of cancers, which is now being widely studied to reveal the prognostic indication of the disease. (thejcdp.com)
Squamous cell3
- [ 1 ] In the United States, sebaceous gland carcinoma is the fourth most common eyelid tumor after basal cell carcinoma , squamous cell carcinoma , and melanoma , and it represents 1-5% of eyelid malignancies. (medscape.com)
- Excretory stem cells give rise to squamous cell and mucoepidermoid carcinomas, while intercalated stem cells give rise to pleomorphic adenomas, oncocytomas, adenoid cystic carcinomas, adenocarcinomas, and acinic cell carcinomas. (medscape.com)
- Squamous cell carcinomas arise from excretory duct cells, pleomorphic adenomas arise from the intercalated duct cells, oncocytomas arise from the striated duct cells, and acinic cell carcinomas arise from acinar cells. (medscape.com)
Stem5
- Embryonic stem cells or induced pluripotent stem cells (iPSCs) are also utilized for AKI recovery. (biomedcentral.com)
- Therefore, we resorted to other types of stem cells. (biomedcentral.com)
- Yuan ZX, Mo J, Zhao G, Shu G, Fu HL, Zhao W, Targeting strategies for renal cell carcinoma:from renal cancer cells to renal cancer stem cells. (ijsurgery.com)
- Retention of labeled DNA strands may be attributed to the ability of stem cells to retain the parental DNA strand during asymmetric cell division ( Cairns, 1975 ) or to quiescence of the stem cell population such that the DNA label is not diluted by frequent cell divisions ( Klein and Simons, 2011 ). (frontiersin.org)
- Two theories predominate: the bicellular stem cell theory and the multicellular theory. (medscape.com)
Vascular1
- Hemangiomas exhibit both a proliferating phase and an involuting phase, whereas vascular malformations are more stable and fail to regress. (medscape.com)
Progenitor1
- MaSC/progenitor cells were identified based on their ability to retain bromodeoxyuridine for an extended period. (frontiersin.org)